GrainGenes Sequence Report: BV211743
Sequence
BV211743
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC1159 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC1159
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'genomic DNA' Locus Comment: On Jan 4, 2005 this sequence version replaced gi:56847490.; Synonyms: XBARC1159; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: CGCTAGTGCCCAACCCGTTATG; Primer B: GCGTAAAAGTTGACCGTTGACCAGT; STS size: 270; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 55 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
primer_bind 157 178 Location: 157..178 STS 157 604 Location: 157..>604
DNA
cggcggtcgccggcacgcgcccgctcgccacctcccaccgccgtccacag
ctcctccctcgccgggcctcgcccctccggcccgcgtgtgctttgccgct
taggccgcggctgagctgggcgggtgcccaggcgtgtttgccccgcacac
ggcgcccgctagtgcccaacccgttatggcccctggggccattgacaacg
gggccccacgcctagaatgttttcattaaaaaaagaaataataataataa
taataataataataataacaattaattaattaattaattaacttaattaa
cttaatcaatctagttaattaaacctaattaacctgctgataaatctaac
tattgtcaattagataacagcctatgacataccggacccacccattagtt
gactggtcaacggtcaacttttaccgcttgcatcatgctgatgtcatgat
gacgtcataaatgccttttgatttaattaatactcttaatcctaaaaatg
atttaaatcttttaaaattaatataaaataagtcgtagctcggatgaaaa
aactttgtacatgaaagttgctcagaatgacgaggcgaatccagatgcgc
agcc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211743
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||