GrainGenes Sequence Report: BV211753

Sequence

BV211753

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum aestivum (bread wheat)

Keyword

STS

Species

Triticum aestivum

Clone Library

Wheat var Chinese Spring Genomic DNA

Data Source

genbank

Release 145, Dec 15 2004

Title

BARC185 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.

Probe

BARC185

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'genomic DNA'

Locus Comment: Synonyms: XBARC185; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: CCGGAGATGGAGATTCGACGAG; Primer B: GCGTATCTCTATTCCCCACCCCGTCT; STS size: 100; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 55 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3.

Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript

EMBL Feature

primer_bind	312	333	Location: 312..333
STS	312	410	Location: 312..>410

DNA

ccgcacggatcgaaaaacacgaccggaaccgcggatctggatctagaacc
tccgcacgtctacattggtactaccgttgatcaaacggaactattttgac
aatccaaagaacatgcttactacactaccacgctcctatgaatccacttg
cacaaacttagccaaaaatctcaagaacaccacatgcatctccccaatac
ccagaagcacaagaaaggaagaaaaagagaggaacttggggaaaaacctt
ggtccatggcaagaggacgaggtggggaacgatcccactggtcggaatct
gaggagagcggccggagatggagattcgacgagggcctccggcgccgttc
ttgggcaggagagagagagagagagagagagagagagagacggggtgggg
aatagagata


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