GrainGenes Sequence Report: BV211843
Sequence
BV211843
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC329 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC329
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC329; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: TGGAGACGTATCAGACCCCTCT; Primer B: GCGGAATGGTGTGACATGTGTAGTAG; STS size: 189; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 60 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
primer_bind 23 44 Location: 23..44 STS 23 702 Location: 23..>702
DNA
caatcaaaaattatagatacgttggagacgtatcagacccctctcgcccc
ctctcttcctctctctctctctctctccgtcgctagctctctctttctct
cgctctcacacccctctcccacacacacacactcgatgtctcttccaaat
agtctgctccccccgcccgcacccgtgctatctcgctactacacatgtca
caccattcccccttcccttatactaggtttacatcatcagttgtctctct
actccacatacactctctccctctcacacacaaacgcgtgcacaaacaca
catagacacgcacaaacacccatttatctagatcctcatctctcctcatg
tccgcatgtgtatctcgcacactcactctctaattatgtatatgtgtctc
cacgttacacaacacaaagccccatgtacatgtctctctctatcctccac
acacatggacacaaagaatctgttatcattgcctcagtctctaacatatg
cactctctctctctctctctctctctctctctcgcaaacacgctcaacaa
tggtttcctcctgaataacttactgtctttcatcaacagtcgtggcagta
cggcgaacacgagacgtgaaaaagaataaatctacacgtgcttagaccac
ctagtatgactactaggactagaacaagctaaaaagcgtgccgccatcac
cc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211843
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||