GrainGenes Sequence Report: BV211850
Sequence
BV211850
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
STS
Species
Triticum aestivum
Clone Library
Wheat var Chinese Spring Genomic DNA
Data Source
genbank Release 145, Dec 15 2004
Title
BARC73 Wheat var Chinese Spring Genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Probe
BARC73
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'genomic DNA' Locus Comment: Synonyms: XBARC73; Contact: Dr. Perry Cregan; Soybean Genomics Improvement Laboratory; United States Department of Agriculture; BARC-WEST, Bldg. 006, Rm 100, 10300 Baltimore Ave., MD 20705, USA; Tel: 301-504-5070; Fax: 301-504-5728; Email: creganp@ba.ars.usda.gov; Primer A: GCGTGTCGTGCTTGTTCTCGGTTCTCAG; Primer B: CGCTATTTGCCGCCACCTCCATCA; STS size: 205; PCR Profile:; Presoak: 95 degrees C for 3.0 minute(s); Denaturation: 95 degrees C for 45 second(s); Annealing: 60 degrees C for 45 second(s); Polymerization: 72 degrees C for 45 second(s); PCR Cycles: 35; Thermal Cycler: Perkin Elmer TC or MJ PTC-225 or MBS; Satellite 384; Protocol:; Template: 30-100 ng; Primer: each 1.5 uM; dNTPs: each 200 uM; Taq Polymerase: 0.05 units/ul; Total Vol: 10 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. Note: Vector: pBluescript; V-type: Plasmid; Genomic fragments cloned into pBluescript
EMBL Feature
STS 1 440 Location: <1..>440
DNA
accctggatgtcgtgcttgttctcggttctcaggggagagagatagatag
atagatagagatagagatagagatagagagagagagagagagagagagat
ggaccgtttgggctaaatgggaggcatctttagggggtcgagtggtgatg
ttgcagctcatatggcgggctatgccgacggtaattgttgatggaggtgg
cggcaaatatggacgatgagctcgattccttatgattgggacaactcacc
tagaggaatttggtaggaagagagaggggcgagagagaggagaggacatg
agagaagtggtaggaggggcccgacggcgcgggacaccctggcgagccag
ccattgcccgcgtgcactgccgccaaggtgagagagagatggtatgtcca
ttttgcatcatgttttcatatcaatttattgcattatgga
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BV211850
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||