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GrainGenes Sequence Report: BV693735

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Sequence	BV693735
External Databases	Data at GenBank Data at EMBL Data at DDBJ
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	STS
Species	Triticum aestivum
Clone Library	AWW CAPS marker from genomic DNA
Data Source	genbank Updated Nov 2006
Title	AWW3A AWW CAPS marker from genomic DNA Triticum aestivum STS genomic, sequence tagged site.
Gene	LVR-3a
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'genomic DNA'; Feature: STS: gene = 'LVR-3a' Locus Comment: Contact: Thorsten Schnurbusch; ACPFG, Australian Centre for Plant Functional Genomics; University of Adelaide, Waite Campus; PMB1, Glen Osmond, SA 5064, Australia; Email: thorsten.schnurbusch@acpfg.com.au; Primer A: TGTTCATTGGTCTTCTTTGCCATAG; Primer B: AATGGTTGCGCCCTTCGTTCCCGCC; STS size: 1300; CAPS marker; Template- genomic DNA; PCR- Hot-start Immolase; (Bioline), AnnTm 65C for 35 cycles; Restriction digest- Hin4I,; giving cleavage products of 600 and 700 bp from Cranbrook-like; fragments and an uncleaved product of 1.3 kb from Halberd-like; fragments. Note: CAPS marker
EMBL Feature	primer_bind 1 25 Location: 1..25 feature_gene 1 1422 put. wheat ortholog of O.sativa: gi|53792931|dbj|BAD54107 Location: 1..1422 STS 1 1422 Location: 1..>1422
DNA	tgttcattggtcttctttgccataggcttcacaccgcctggcctaaaaca tccagaagtcttgaaagtcccatctgcacgcaggtcaggcgaatccatcg aaaaaaatctcgaaccgcctcttcggcagcagcacgcacgagagaccggt taaccaagaaaacggtccaaagtactttaaacgcttcagcaaaaaaaaaa atggaggagctaagcttataacacattcgggcaacaaacaaaaggtttag tcaaaaagtagtcgtcgggcattttgacagacaaacgctgcacaaaaaaa actgctttaattaatcaactggtcaactaatttgaactgaaatctgcata aaaatttttgtaaaatatctcacattaaaagctccaaacacagcactata aacagaagtaccttaacaactcaaatatgttaaataaatttcatatatgt tcttaccccttcagggggaggagggttctttgtgaatttagtgccaccag gagcccatggaactgcaaagtaaatacattgcataacctcatcgatcaga agaatcacagcataccaaaccccaaaatcacatttgttaaacaggtaaca tagggtaagaaagcaattgcatcaaaggaaagcgcacatgagcagtttgt tacaggtagtatgatcttaagatgctatagtaccgaaaataacctatctg aaaatgtgtcatttcttcactttaatctaaagcctargtacattatcttc gattatgaaataaaagtatgcgcaacaatttttattttatttctaaaaaa aaggctattttgcacaacagagtgtgtatcataaagctgaagcagcctct attctcaaggataaactgatcgtaaataaagtttcgcctgaataagaata gcgtgacactgaaatgaatatgtaacaagtctgtacatttctggcctgag aatgttacgtttgcagtgaaggttagcattgaatacaacatgaagatcat gtcactactcactacagcagttagttgagactaaacaacacagctcacgc ctcaagcaaatattggtatcctaagataatctgaattatttcttagagag gaggatactcctctattactgaaagctaataagtagttctacaaaccagc aaaatgaaataacaagataatgtgatgaattaagtgatgacaagacatgt ctccaagactccaagtagctccacgcctctaactatacacaatcaatcat taaaaaatgcaggcttgagctcacagcttcagtaaaacatacaaattcat cgggcctgagtgatcaaaaacattcccaactgaatgatcagtgactcaat gttcaacagcgtaacgaaacaactgcaagtatcacgctacatgattaccg cgcagtatcgctgccatacgtt

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