GrainGenes Sequence Report: CA000154
Sequence
CA000154
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC161872
NCBI UniGene
Hv.9509
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf61p16, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HS09O11u HS Hordeum vulgare subsp. vulgare cDNA clone HS09O11 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HS09O11
Probe
bags5n14
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 550 Std Error: 0.00; Plate: 9 row: O column: 11; Seq primer: M13uni. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gatccaccagctgacgacagatatatatgatctaaagtttaacatctgtt
acatcaccacaaccatcatcaaaacatagtctcaggtagcaccacataca
aacagcaagatacacatggggagaaaataatgcggctcccttcacagcaa
gggcaagtagcaaaacattacccaaaatttagagcttgtcctcgaaatcg
gataggggagtcatctgctccttgagacccttcctcttgcggatctccgt
cacaagcgtcgcagactgggtgccagggtccaaaggatcagaagccatga
tgtcccagtgatcaaacacacactgggggaaggcctggccggacgtcgca
gccctgagggtgctcgagaacccgaaggactcgatgacaggcaggtaggc
cttgatgttgtagagtggagtaccttgcctctgcatctcctcaaacacgt
gtcctctcttctgattcagcacaccatagataccaccaagcgcattctcc
ggcgcctggatctcaaccaggtacacgggctccagcagccttggcttggc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CA000154
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||