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GrainGenes Sequence Report: CA001593

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Sequence
CA001593
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC173794
NCBI UniGene
Hv.19378
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, weakly similar to NP_001058578.1 Os06g0714800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HS
Tissue
embryo + scutellum
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HS05A06r HS Hordeum vulgare subsp. vulgare cDNA clone HS05A06 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HS05A06
Probe
GBM1494
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 447 Std Error: 0.00; Plate: 5 row: A column: 6; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggggcacaccacccgagcgcgcacgcacgcactctccacctc
ccaggagcaagagcaacggcggccgtgccacgcgtgcgcgagcatacgca
tgggtgaagaggccgcctccaccgcggcgccggcgtcgctctcgtcgtcg
tcgtcgtcgtcgtcgtcgtcgctgctggccgcggcggccgtgaaggggga
cgaggagcggtacgtcaaggtggcgtcgaggttcttccgggtgaggccgg
gcggtctggcccgccgccaccacttcctcgactcctgcttcctctgcaag
gcgagcatctcccgcgaccgcgacatcttcatgtacaagggcgacgaggc
gttctgcggcgaggagtgcaggcaggagcagatggccatggacgaggcgc
tccacgcggtggcgcgccggcgccacaagcaggtcacggccgagcag

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