GrainGenes Sequence Report: CA004840

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Sequence

CA004840

External Databases

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf151h09, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

embryo + scutellum

Developmental Stage

0-16 hours after imbibition

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HS18N12r HS Hordeum vulgare subsp. vulgare cDNA clone HS18N12 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HS18N12

Probe

UMB604

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 459 Std Error: 0.00; Plate: 18 row: N column: 12; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

gaaaggaagccttcatctcggagcaatcccctcatccaattcgcccaaat
ccgcatcaggctgctaacagagagagagagagagagatgggtagggcaag
aaggggaatggcgggcaagggcaagaggaggccgcgtgctgcggcgaagg
cggcggccgtggccggcgacggacacgaggaaaccaaagacgggcctgag
aaagccgccgccggcgggggcggcttcttctgctgctacctcctccggtc
gctctgcccgcgcagcaagagccgcacctacatcgggttcacggtgaacc
cgcggcggcggatccggcagcacaacggcgagctccggtgcggcgcctgg
cggaccaagcgcggccgcccctgggagatgatgctctgcatctacggctt
ccccaccaacgtcgccgccctccagttcgagtgggcgtggcagcacccaa
ccgagtcgc


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