GrainGenes Sequence Report: CA009757

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Sequence

CA009757

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, moderately similar to NP_189295.3 ERF1-3 (EUKARYOTIC RELEASE FACTOR 1-3); translation release factor [Arabidopsis thaliana]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

endosperm early

Developmental Stage

0-16 hours after imbibition

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HT12O17u HT Hordeum vulgare subsp. vulgare cDNA clone HT12O17 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HT12O17

Probe

bags15e12

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 556 Std Error: 0.00; Plate: 12 row: O column: 17; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

gaatgaatcccaatccaattcaaactttcccgcatgacaacaataataag
tgccttacaacaactcaaatttagggcagacatttgccatgttccaccat
tagggaagacaagttgctaattcgaaccttagacacagagtcagttcaga
caacggcagacaaggagttcccggggaaaaaagattggcaaaaataagag
gcaacaaaaggttcggtacacggttatcattgcgatttaggataacatct
cgacatcttttgatagaggaattgtggctgtatcgtctcagacttctaaa
gcatggtatgaagctgacccgcgcgcatcagtcactggatagcttctttg
aggtgcccgggaacaaatagtcgcgcaaaattcagatttaaccgcgactg
cttcaggcatgtgattgattggttgctagtcagaatcttcatagacaccc
tcatcgtcatcaagatcatcaaaggaacgaatgtccagctgatagcgcag
gataccaccaatgccaccaaaccctctacagaactgtgatccctcctgag
atttat


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