GrainGenes Sequence Report: CA010396

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Sequence

CA010396

External Databases

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'M12 protein'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

endosperm early

Developmental Stage

0-16 hours after imbibition

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HT10F13u HT Hordeum vulgare subsp. vulgare cDNA clone HT10F13 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HT10F13

Probe

GBR1600

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 432 Std Error: 0.00; Plate: 10 row: F column: 13; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

DNA

gcttgaatgcttgatggataattctaccgaagttcatagaccactctgaa
aagaaaagaaaaaaagtcgagtacgagctcagaaatgctgctgcaagggg
agaaaagaaatgctactatcgattgacaatttgggggcagatgttcagca
agagagagtacaccagaaagaaaaaagcagtaactgatcttacattgttc
aaataaatgccacagagatgacaaaaaagggttctctagttcgaaacaaa
caaacaaaaaatagataaccggcttatctccgccaactttattggggaaa
aggggcttagttgaacggagagcgcatctggtagcagccgcgtacgcacg
cagcagcgtatacaatcgatgaggctttctctgaatttttcctcaaaaca
atcagggtgagggataccaagaatctccttcg


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