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GrainGenes Sequence Report: CA014281

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Sequence
CA014281
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC183453
NCBI UniGene
Hv.12943
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001050108.1 Os03g0349200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HT
Tissue
endosperm early
Developmental Stage
0-16 hours after imbibition
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HT10O17r HT Hordeum vulgare subsp. vulgare cDNA clone HT10O17 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HT10O17
Probe
GBR1601
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 539 Std Error: 0.00; Plate: 10 row: O column: 17; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cggcacgaggcaagaaggtgcgcttcgacaacctcgagcccgatgcgtgc
ggttatggcccgggagatcctcatcctgcgccgcctcgaccaccccaacg
tcatgaagctctacggcctcgtcacctcccgcatgtcctgcagcctctac
ctcgtcttcgactacatggtccacgacctcgccggactcgccgccagccc
cgacatcaagttcactctgccacaggtcaagtgctatgtgcatcagttgc
tgtcagggttggagcattgccacaatcagggggtgctgcatcgtgacatc
aagggctcgaatctactcttggatgacgacggtgtgctgaagattggaga
ctttggtctggcgtcgttcttcgacccgaaccataagcaaccgatgacca
gtcgcgtggtgacgctgtggtaccggccaccagagctgctgttgggtgct
acggattatggagtgggtgtggacctgtggagcgccggatgtatccttgc
tgagttgctggctgggaagcctattatgccgggacggac

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