Query (optional)   in Class  

GrainGenes Sequence Report: CA015674

[Submit comment/correction]

Sequence
CA015674
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC153999
NCBI UniGene
Hv.9141
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Lipoxygenase 1 protein'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HV
Tissue
germinating seeds
Developmental Stage
germinating seeds (48-96 h)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HV12K15u HV Hordeum vulgare subsp. vulgare cDNA clone HV12K15 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HV12K15
Probe
bastl50E0309
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 496 Std Error: 0.00; Plate: 12 row: K column: 15; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
ggaagcaaatctgctttatttattcggcttaaagagcaactgctgacata
gtactgtcacagacacacactacaccaatctctcgacaagtttcgtacgt
ggcgaacgccctttattcatccatggttgccgatggcttaaattagatgg
agatgctgttggggatgcccttggcggtaagcccggcagcggcgcccttg
tggtcggaggtgttggggtagagcagcatgtagggaaacttagccgggcc
gttgcggttcttgagctccgggtcatggttcatgcccaccaccttgctct
cgatctccaccagccggtcgctgaaccgcttgaacacctccagggccttt
gggtccgaggtccactccggcgtgtcccgctgcccgaggtacagctcgtc
ggaggagtgcttcgacagcacctccagcagcgacacgccgatgatggtct
ggatctggctcgtgatggtgtggatgaaggcccgctccgggtcgcg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.