GrainGenes Sequence Report: CA016509

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Sequence

CA016509

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

germinating seeds

Developmental Stage

germinating seeds (48-96 h)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HV08I08u HV Hordeum vulgare subsp. vulgare cDNA clone HV08I08 3-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HV08I08

Probe

bastl40H1115

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 526 Std Error: 0.00; Plate: 8 row: I column: 8; Seq primer: M13uni.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

DNA

gaaataattagatctaaatgaatgccaaataattagatctagatgaatgc
caaataagtattattattttccgctcaccaccctccaaataaataagatt
acaatgtcagcaacacaacacagaagaagaaaatgcatttaatcaactgg
cataaacaacatacgagtactaattaatacagaaacccttgtaattacat
tctcattcttctcccagtaatttcccccaaaattaatgaccagtaacaaa
tataaaagaaatggctccaacttcaaactaaaaccctggtaaaaccgaga
ggtcggctatcccctttgtcagactcgcaaccttttgtagcagcgccagg
cggttgttacggatcttctcatcttccgccatgacgaaaacattgttgaa
gaaatcctccaggggctgtatcaagagcaaagaagactcggcgaaggttt
tgacgtcgacgccaggatggatcttattggcaacctccaagtaggcgctc
cacagggctttttcttcgtctttctc


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