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GrainGenes Sequence Report: CA017060

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Sequence
CA017060
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC170765
NCBI UniGene
Hv.23380
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Hypothetical protein LOC548250'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HV
Tissue
germinating seeds
Developmental Stage
germinating seeds (48-96 h)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HV05A01u HV Hordeum vulgare subsp. vulgare cDNA clone HV05A01 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HV05A01
Probe
BaAK28A10
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 372 Std Error: 0.00; Plate: 5 row: A column: 1; Seq primer: M13uni.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb
DNA
ntagataaacatgggttcctccattaattttactgcatataaatcgataa
aaaggagtacaagccctgcttgatcgatgagaaatgcatctgatccgagc
acacgaacactacagcagggcatccaagcccgtccacccacccacgcacg
cacacacaatgggaaacagcccaacatccaggccgcatctccccccgtag
taataggatcgacgatgatgatgatgatgatgcgtcgttaacccgcgcgg
cgctcggcgctcgagcgccccctgccttccctcattagtcgttatacgta
gcatagcggttttccttccatggctggcgggaccaggagggtcgagctga
tcgggggcttcaagcccgggcg

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