GrainGenes Sequence Report: CA023524

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Sequence

CA023524

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, strongly similar to NP_565812.1 UBQ7 (RELATED TO UBIQUITIN 2) [Arabidopsis thaliana]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Tissue

pericarp

Developmental Stage

0-7 DAP (days after pollination)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HZ46I04r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ46I04 5-PRIME, mRNA sequence.

Strain

lab_host XL10-Gold

vulgare

Clone

HZ46I04

Probe

GBS822

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 506 Std Error: 0.00; Plate: 46 row: I column: 4; Seq primer: M13rev.

Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp

DNA

gccgcgagggagaggaggcgaaaaacctcggaggcggagggagaggggac
taggaggagatgcagatcttcgtgaagacgctgacggggaagacgatcac
gctcgaggtggagagcagcgacaccgtcgacaatgtcaaggccaagatcc
aggacaaggaaggcatcccaccagaccagcaaaggctaatttttgctgga
aagcagctggacgatggacggacattagccgactacaacattcagaaaga
gtcgacacttcatttggtcctgaggctcaggggtgggactatgatcaagg
tgaagacactcacgggtaaggaaatcgagattgacatcgagcctaccgac
acaattgataggatcaaggagcgtgtcgaggaaaaagagggcattccccc
tgttcagcagaggctcatttatgctgggaagcaacttgctgacgataaaa
ctgctaaagattacaacattgaaggtggttcagtgctccatcttgtgctt
gctctg


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