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GrainGenes Sequence Report: CA027247

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Sequence
CA027247
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC192567
NCBI UniGene
Hv.27343
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf82n10, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HZ
Tissue
pericarp
Developmental Stage
0-7 DAP (days after pollination)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HZ58F06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ58F06 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HZ58F06
Probe
GBR1848
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 444 Std Error: 0.00; Plate: 58 row: F column: 6; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp
DNA
gagaggctgctgacccacccaccccaccccaccccacctccctgctctgc
cctgctctgccattacttggcttggagtgtgctgctgctgctggggtgaa
caggagagagaagaagcatagaagagcaagcagtgagaactctgactgag
agagagagagcgagagagagggagaagaggaagaaggaggaggaggagga
gatggaggtgcaggttcgccatggcttccttccaacaggcaggcagcagc
acctcctccatggccaagctcaccatcagcaggaagctcccgcaggtgaa
gatgagtggtgggagtacttcccctgcccctactgctacatcgaggtgga
ggtccccttcctctgccaccacctccaggaggagcactgcttcgacatga
agaacgccgtgtgcccgatctgcgccgacaacctcggggccgac

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