GrainGenes Sequence Report: CA031926
Sequence
CA031926
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC182849
NCBI UniGene
Hv.11094
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to NP_001051846.1 Os03g0840200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
HX11I09r HX Hordeum vulgare subsp. vulgare cDNA clone HX11I09 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HX11I09
Probe
GBS576
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 623 Std Error: 0.00; Plate: 11 row: I column: 9; Seq primer: M13rev. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gaggaattcggcacgaggagagagagagcaccgaggtggacgtggtggcc
gtgtccctcggcgacgacaacgacgacgacggcagcatctctcaggtctc
catcaagaagctcaccgtcggaggcgagaacaacgcgttcccgtcgccat
cgccggacgggaaatggctggtgttccggtcggggaggtccgggcacaag
aacctctacatcatggacgcggaggaaggcgaggccggcggcatccggcg
cctgacggacggcccatggaccgacaccatgtgcaactggtcgcccgacg
gcgagtggatcgccttcgcctccgaccggcacaacccgggaggcggcagc
ttcgccatctacatggtgcacccgaacagcaccgggctgcgtagggtggt
gcacagcgcggacggcggcaggacaaaccacccttggttcagcccggact
ccaagcgcatggtgttcacctcggacctcgccgccgtttccgccgagccc
atttcgaacccgcaccactaccagccctacggggagatattcaccatcaa
catcgacggctcggggctccagcggctcacgcacaactcgttcgaggacg
gcacgccgtcgtggacgccgcac

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CA031926
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