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GrainGenes Sequence Report: CA032410

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Sequence
CA032410
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC170045
NCBI UniGene
Hv.23439
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001063570.1 Os09g0499000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HX12P07r HX Hordeum vulgare subsp. vulgare cDNA clone HX12P07 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX12P07
Probe
UMB016
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 624 Std Error: 0.00; Plate: 12 row: P column: 7; Seq primer: M13rev.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cttatcccatcacccatttcttcctcttccctctctttgggctctctctc
tctctcttacaagttgctgctgagttggtgtttgtccttgttcttggacg
gacaaatccatctcccattcgagatttgctccgagggcggcgctggcatc
atcgtccggatcgtcggtttcgcggcgcagacatgtcgacggcggtggcg
gaggctttccagtcctacggtgggttccccggatcggggaggaccaaggg
ggacgcgctgctcggcaagaagatgagcgacgggttcttcatcgaggagg
aggaggaggatgaggcggaggaggtgctgaccgagagctcctccatcggc
gcgccgtcgccgtcgagctcctccatcggcgaggactcctcgtcggaggt
tggtggcgatggtgaagacgatgaggtggagagcaagctcaaggaggagc
ccgggctcggctgcctggacgccttggaggactccttgcccatcaagaac
gggctgtcgagcttctacgccggcaagtccaagtccttcaccagcctcgc
cgaggcggcggcgcgggacgccgtgaaggagctggccaagccggagaacc
cattcaacaagcgccgccgcatcc

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