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GrainGenes Sequence Report: NSFT03P2_Contig8595

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Sequence	CA500558
Contig	NSFT03P2_Contig8595
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC301745
NCBI UniGene	Ta.10464
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001118998.1 unknown protein [Arabidopsis thaliana]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat meiotic anther cDNA library
Tissue	Anther
Developmental Stage	Meiotic stages pre-meiosis-metaphase I
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE4021_C12_E23ZT Wheat meiotic anther cDNA library Triticum aestivum cDNA clone WHE4021_C12_E23, mRNA sequence.
Strain	lab_host E. coli DH10B
Clone	WHE4021_C12_E23
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: T7 primer. Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging , and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: pSPORT1; Site_1: SalI; Site_2: NotI; Plants were grown in a glasshouse. Anther meiotic stage was determined by removing anthers from individual primary florets. One anther was sacrificed for microscopic staging, and if determined to be between (and including) meiotic stages pre-meiosis and metaphase I, the remaining two anthers were collected and pooled for library construction. The tissue, total RNA, and poly(A) RNA were prepared, cDNA synthesised, and directionally ligated into pSPORT1 by Tim Sutton in the P Langridge Lab at the Department of Plant Science, University of Adelaide, Waite Campus, Australia. Average insert size 1.5Kb. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	taccggtccggaattttcggtcgacccacgcgtccgcgcaaatgaagccg ctgtagtacttcttccttcaaacatcacagtctttcacactagacttttc cggttcaggtctatcggacggagatcatgtcagccttggttggcatgaga aagaggacctcaaatgtgcagtatcattcctgcgcaccaacaagcaagtt tcccgtatacgcctttgggggcgatcgatgggtgctgttacaagcctact atatggagcagaagatccctcaattgctggcatggtattggatagtgctt tcaccaacttgtatggcttgatgttggaacttgtggatgtttacaaaatt ccagttcctaaattcacggataacatggctgtacagtacatgcgacgaac cattcagaaaagagcgaagtttgacataatggaccttaatgttgtaaagc ttgctcccaagacatttattcctgcattatttggacatggtctgagtgac atgtttattcagcctcaccactgcgatcgtattcaacgaagcatatgggg gggataaaagtatggtaaaatttga

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