GrainGenes Sequence Report: AL499893

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Sequence

AL499893

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Hordeum vulgare Barke etiolated leaves

Tissue

etiolated leaves

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

AL499893 Hordeum vulgare Barke etiolated leaves Hordeum vulgare subsp. vulgare cDNA clone HK03P12r 3', mRNA sequence.

Strain

lab_host XL1Blue

vulgare

Clone

HK03P12r

Probe

GBS28

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:

Note: Vector: pBluescript SK-; Site_1: SalI; Site_2: NotI ; mRNA was made from etiolated leaves of spring barley variety 'Barke', a high quality malting variety. Plants were grown on filterpaper for 6 d at 25 C in the dark. Leaves were harvested at daylight conditions. Sal-Adaptor Sequence: TCGACCCACGCGTCCG Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp.

Note: Vector: pBluescript SK-; Site_1: SalI; Site_2: NotI; mRNA was made from etiolated leaves of spring barley variety 'Barke', a high quality malting variety. Plants were grown on filterpaper for 6 d at 25 C in the dark. Leaves were harvested at daylight conditions. Sal-Adaptor Sequence: TCGACCCACGCGTCCG Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp.

DNA

caaggcagacccgttatcagagacgaggaccgacgaggaccacatctatg
tgccccgggacgaggcattctcggagcgcaagcaagcagcgttcaacacc
aagctcaacttttccaagttgcacgcagtctccacatatatgaagacgtc
caagcacaagggccagagcttcccctccttggcggccatcgacgccatgt
acgaggaccgatccaatgaaccagccgcagcagaaactaaatggatggtg
tgccaccttggatttgctcgagaaggagttgtcccacctcgttcatctcg
aaggggcgatgttcctgnaggagatacgcaaagtcatcaagtttgaaacg
ccggaaattcaagaca


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