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GrainGenes Sequence Report: AL500134

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Sequence	AL500134
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC181368
NCBI UniGene	Hv.23088
DB Remark	Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Actin'
Keyword	EST
Species	Hordeum vulgare subsp. vulgare
Cultivar	barke
Clone Library	Hordeum vulgare Barke etiolated leaves
Tissue	etiolated leaves
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	AL500134 Hordeum vulgare Barke etiolated leaves Hordeum vulgare subsp. vulgare cDNA clone HK05A09r 3', mRNA sequence.
Strain	lab_host XL1Blue vulgare
Clone	HK05A09r
Probe	GBR394
Remark	DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http: Note: Vector: pBluescript SK-; Site_1: SalI; Site_2: NotI ; mRNA was made from etiolated leaves of spring barley variety 'Barke', a high quality malting variety. Plants were grown on filterpaper for 6 d at 25 C in the dark. Leaves were harvested at daylight conditions. Sal-Adaptor Sequence: TCGACCCACGCGTCCG Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp. Note: Vector: pBluescript SK-; Site_1: SalI; Site_2: NotI; mRNA was made from etiolated leaves of spring barley variety 'Barke', a high quality malting variety. Plants were grown on filterpaper for 6 d at 25 C in the dark. Leaves were harvested at daylight conditions. Sal-Adaptor Sequence: TCGACCCACGCGTCCG Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp.
DNA	ttttttttttttttttttttttctttaaaaaatccataatttaccatgat tctcccctcagagcaatagtaagatataaaaatacatagcaaaaaccatg agctattacagacaattcccaacaacaaatcaagccaacccaagtttgac aactgatcccacaaacacactgacaatgcacaaacatagatagtgtactt gcacttgcaccatggagccgccacgtatataggtggcgtatcccgagagt acccacgtcacgtctggaaaaaaacatagcttgcaaatacgcaatacatt tctagaagaatgaaccaaacataaccgaagactagatgataacagcagtg gagcgctcgtggagatcagaagcacttcctgtggacgatcgctgggccag actcgtcgtactcatccttggatatccacatctgttggaaagtgctgagt gaggctaggatggaccctccgattcagacactgtacttcctctcaagggg agcgacgaccttgatcttcaagctgctcggagcaanggcggtgatccctt gctcatacggtcaacgatacctggggaacatanttgtgccaccactggag cacgatgtttccat

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