GrainGenes Sequence Report: AL500325
Sequence
AL500325
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC189615
NCBI UniGene
Hv.30441
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus, moderately similar to XP_002263418.1 PREDICTED: hypothetical protein [Vitis vinifera]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke etiolated leaves
Tissue
etiolated leaves
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
AL500325 Hordeum vulgare Barke etiolated leaves Hordeum vulgare subsp. vulgare cDNA clone HK06B11r 3', mRNA sequence.
Strain
lab_host XL1Blue vulgare
Clone
HK06B11r
Probe
GBR372
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http: Note: Vector: pBluescript SK-; Site_1: SalI; Site_2: NotI ; mRNA was made from etiolated leaves of spring barley variety 'Barke', a high quality malting variety. Plants were grown on filterpaper for 6 d at 25 C in the dark. Leaves were harvested at daylight conditions. Sal-Adaptor Sequence: TCGACCCACGCGTCCG Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp. Note: Vector: pBluescript SK-; Site_1: SalI; Site_2: NotI; mRNA was made from etiolated leaves of spring barley variety 'Barke', a high quality malting variety. Plants were grown on filterpaper for 6 d at 25 C in the dark. Leaves were harvested at daylight conditions. Sal-Adaptor Sequence: TCGACCCACGCGTCCG Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp.
DNA
atccacnagccgagccagcttaccaaggtcaccaggcttcctgctgaccc
tttcatgtgtatgggaaagatctctgacattatancccatggaattcctc
ccatacccagtgagaaagatccagtgaagaccagaatccctgccaaagcc
ncaccacgttgagctccttcgcccaatgatgctccttggacaggaatgac
aggccaactagtaggcaacccaangcatgttccggctgcagagaccatta
gaagtggcctccttccagccttgtccatcagaagcaccccaagtgccgtt
catcggaatctgaaccgcgaccataagcgaagcattccttgtg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: AL500325
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||