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GrainGenes Sequence Report: AL502421

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Sequence
AL502421
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC162495
NCBI UniGene
Hv.2066
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf59j02, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke roots
Tissue
roots
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
AL502421 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare cDNA clone HW07I17u 3', mRNA sequence.
Strain
lab_host XLOLR
vulgare
Clone
HW07I17u
Probe
GBR1150
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:
Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA
ttttttttttttttttttgggttccgtaagtagcatccaaattatcgttg
tgacgataaaagcccaacacggactgaatattttacagtaccttgcatca
cagaataacaacaaatatttcaaatttgaccatataaggaacactacaat
acagtcaagtaaaatatctgaacattcttactttactgtccaaaatactt
gaaccacaggcgagattcatatctgacttgttctatttctctcagatcat
gtctttatgcgcaccacgaaagaacatgctggaaggtttgacatatctgc
agagacagcacatggaattcccaagtttgtgagagcacctcttattaacc
cacatgggaaatataaaagcatgcttgttgtttgggctgcagaatcacct
aatgacccagagtcattctcagttgcatcaagtgttctctactgctggat
ctaagagaaacacgagtaagccatcggaaatgattatcttgaagaacgaa
ggtanccctatgatttgttttcagattgtcaatcctgcttcttgaacagt
tctgaccaaaaatctttgcaaataaatttggattggcctcaagaatgat

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