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GrainGenes Sequence Report: AL502467

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Sequence
AL502467
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC176600
NCBI UniGene
Hv.21248
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke roots
Tissue
roots
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
AL502467 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare cDNA clone HW07K21u 3', mRNA sequence.
Strain
lab_host XLOLR
vulgare
Clone
HW07K21u
Probe
GBR1152
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:
Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA
ttttttttttttttttttcaagcagaaatcaatcatttggggaagcacgt
cagaaaccatcttttcaccaagctgctctcactgatcccgatcgactccc
gaccctggacccaacaccttccaaccaaacaacgagcacttcaacagtat
aaatcacaagttgaactgagctttcagctggtctccaaagctgacgataa
agatctctctgattgcgaaacagtgatgcaacagcaacaccctgaagact
ggagacacgacacgttttatttcacgaggccactcgatcacccctgcttt
cccgaaccaaggaaggggcaggcctgcggctgcgcgtcggcacctgatgc
cgacaccgtanccgcctcgcggagcctcccggcgagcgccgcctcctcgt
ccccctccttgggacccttgataagccccgcgaggagctcccctgggtcg
tanaacacctcccacgtcctccgcccgcagctgctcgtccaccttggagc
acggcgacgccgtanaactcga

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