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GrainGenes Sequence Report: AL503946

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Sequence	AL503946
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC154045
NCBI UniGene	Hv.23350
DB Remark	Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Atlas PR17d precursor'
Keyword	EST
Species	Hordeum vulgare subsp. vulgare
Cultivar	barke
Clone Library	Hordeum vulgare Barke roots
Tissue	roots
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	AL503946 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare cDNA clone HW03O22T 5', mRNA sequence.
Strain	lab_host XLOLR vulgare
Clone	HW03O22T
Probe	GBR602
Remark	DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http: Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA	gcgttggcgtcgactacgccaagcaggtgctctccgatgcctcgtccttc atctggaccaccttcaaccagcccaacccgggagaccgtagggactacga ctcggtcaccctcgctgtcgtcgacaacatcgagcctgtggcccagaccg tcggcaacgctatccaactccgagcccaatacgtcgccggcttcgacggc gacgtcaagcaagaggtgaaaggcgtactgtaccacgaggcgacgcacgt gtggcagtggatagaccactacggcgaaaaaccggggctcttcgagggga tcgccgactacgtgcggctcaaggccgacctcgcgccggggcactgggtg aaggacgggggcggcgaccggtgggatcaggggtacgacgtgacggccag gttcctggactactgcgactcgctcaagccagggttcgtggcggagatga acggcaagctcaaggacggctacagcgacgactacttcgtgcagatcctg gggaagagcgtggacgagctgtggagcgactacaaggccaagtaccccca gccccagagctgatggtcgacgtacgatgcatgcagtttattggtgtatg tg

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