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GrainGenes Sequence Report: AL505735

Sequence
AL505735
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC161426
NCBI UniGene
Hv.3057
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf87f13, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke roots
Tissue
roots
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
AL505735 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare cDNA clone HW09F22V 5', mRNA sequence.
Strain
lab_host XLOLR
vulgare
Clone
HW09F22V
Probe
AL505735
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:
Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA
gcgagcngctagctggcacagtcccctaccaccaccaccaccagcccact
ctatcagcctcgggtgaagccgagcagatccatggcgcagtctcgtcgca
tggccgggtccgccctcctgctgctgctcctcctcgtcgccacagagatg
ggggcgacgacggtgaaggtggcggaggcgcgggactgcctgtcgcagag
ccacaagttcaagggcacgtgcctcagcagcagcaactgcgccgccgtct
gccgcaccgagaacttccccgacggccagtgccacacccagaacctcgcc
cgcaagtgcttctgcaagagggtctgctagatctgttgctgctgttggtc
agtgccgttcgctagatctgttcgtcagtagctgcctgcagttcgtgtga
ctgttgccccgtaataaagttaggaaatcaataaccggggtctcggtagt
ttgtgttcgccgcacgtcgtg

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