GrainGenes Sequence Report: AL508047
Sequence
AL508047
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC172907
NCBI UniGene
Hv.5462
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf65o01, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Tissue
developing caryopsis (3.-15.DAP)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
AL508047 Hordeum vulgare Barke developing caryopsis (3.-15.DAP) Hordeum vulgare subsp. vulgare cDNA clone HY07J23V 5', mRNA sequence.
Strain
lab_host XLOLR vulgare
Clone
HY07J23V
Probe
[Bcl1318ct1882cn5763] {SpCl-454}
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http: Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from developing caryopsis (3.-15.DAP) of spring barley variety 'Barke', a high quality malting variety. Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA
tactncggcggcggcggcggacggcggcgatgagcacgtggaactacgtc
gtgacggcgcacaagcccaccagcgtcagccactcctgcgtcggcaactt
caccggcccccaccagctcaacctcatcgtcgcgaaatgcacccgcatcg
agatccacctcctcacgccgcagggcctccagccgatgctggacgtgccc
atatatgggaggatcgcgacgatcgagctcttccgccctcacaatgaggc
ccaggacttcctcttcattgccacggagaggtacaagttctgcgtcctgc
agngggacgcagaaaaatcagagcttctcactagatccatgggagatgtt
tctgatcgcatcggccgccctacggacaatggacagattgggatcatcga
ccctgactgtanacttattggccttcacctctatgatgggttgtttaagg
ttataccattcgacaacaaaggacagctgaaggaagctttcaatatcaga
ctag
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: AL508047
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||