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GrainGenes Sequence Report: CB875298

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Sequence
CB875298
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC187285
NCBI UniGene
Hv.9577
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001063586.1 Os09g0502200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HX
Tissue
apex
Developmental Stage
apex (3-5 mm in size)
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
HX07O01w HX Hordeum vulgare subsp. vulgare cDNA clone HX07O01 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HX07O01
Probe
UMB706
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 609 Std Error: 0.00; Plate: 7 row: O column: 1; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gaaataggcaactgaacattgcattccagtagtagagatggggacattgg
gtaaaagggttcgtgcaagcgacatgattagcaagacaggagccagtaac
aatgaaatcagagtgactatcatttcaaccgtcaacagatgacgtgcatg
aatgatgattttgatacaacaaaaaggacaacggcctgcctaaccaccac
gaaaaacgaaaaatggtcaaacggacggtcagctgtcactgttacatcac
aaggtttgagaacatcagtaggatcataatacatcaccctcagtgtcctc
cctcaatgtgtgaatcaatcagcccaatgaactcctgaacttgcacgatc
cgtggcttgggtccttgatggtctgctctgcgaggttgttgaagtagcac
gtagcacctgtcttcctgaactgctgccagtaggagttgaaggcgtagct
ggcgtgcgccgcgccggtgttgggctggaagcactcgccgccgggctgga
cggcgtcgcaggtcccgttcccctgcccgcacgcgtacgtcagcgcgtcc
cccaccgccgtctcgttcagcttcctgccggcgtgggcggccagcacgca
ccatatctg

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