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GrainGenes Sequence Report: CB879524

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Sequence
CB879524
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC186587
NCBI UniGene
Hv.19043
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001051742.1 Os03g0823400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HP
Tissue
epidermis
Developmental Stage
seedlings grown in greenhouse for 6 days
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
HP02J06w HP Hordeum vulgare subsp. vulgare cDNA clone HP02J06 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HP02J06
Probe
UMB715
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 381 Std Error: 0.00; Plate: 2 row: J column: 6; Seq primer: T7.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
DNA
ccgctttacacaaggagacatacctgttacagagtcaagtcaacaggaca
agccgcgcctaattaacaaaaaaaaaagtggctggctggctggctggctg
gcatctctggccgtgatgccatcaagattcgtccgtcgccggtggcggta
gttggggtgggaggttggtcagcagtcagctggccggtcagtgggcggcg
atggcgctgcagcgccgctggcagaagttggggacgcggtccatgcactg
gtacacgggcgggtgcacggcgagggcggacttgacgcagtcccggcacg
ccgggtggcacccgccgggcgccgcgtccatgcaccggcactgcggcgag
tccgacttggtgcaccccccgcagctgtcgc

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