GrainGenes Sequence Report: CB881851
Sequence
CB881851
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Hv.30602
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'Transcribed locus'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
HM11B05w HM Hordeum vulgare subsp. vulgare cDNA clone HM11B05 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HM11B05
Probe
UMB007
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 351 Std Error: 0.00; Plate: 11 row: B column: 5; Seq primer: T7. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
cttcggtgatttttcacatttccttatttgagcaattttttcctttcctt
tttcaagcgattttttcaccagccagcgacagctatgcatgcgtatacgc
aataaaatcgcaggaatcaaggtggggtggcactacggagagctaaacca
gtggtgtaagctttaaatgggctcaaataagataaaaagggcaaataatt
aatgtgccaacaatgtgtaccccccccccccacacacacacacaccctgc
cctctcccaagagaagagttggactaaactatcccggtcttccgtcgatg
cggagggtatcgatggctagaacaagagaagaggaagtagttccctcgtg
c

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CB881851
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