GrainGenes Sequence Report: CB881957
Sequence
CB881957
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC164024
NCBI UniGene
Hv.23796
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf104h05, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HM
Tissue
male inflorescences
Developmental Stage
male inflorescences (approx. 2 mm in size), green anther stage
Data Source
genbank Release 136, Jun 15 2003 genbank Updated Nov 2006
Title
HM11G16w HM Hordeum vulgare subsp. vulgare cDNA clone HM11G16 3-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold vulgare
Clone
HM11G16
Probe
UMB205a UMB205b UMB003
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 588 Std Error: 0.00; Plate: 11 row: G column: 16; Seq primer: T7. Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
DNA
gggtgggaatactttgcacttgattgtagcatgattcatacatagtaatt
tcttgctacacaaaatgacacaaatattacaaaccttgggtaaaaaaaaa
ctgacacccactgacccctgaccgtcataacagttattccgaattcattc
tgcaaatagtggaaaacgtggtagggaatgataaaaggccaaaccaaccg
cgcgtgcggcgatcataaatagcaacgtcactcagcatgaaaccatgggg
atttcagtgctgggctagctggacggtcgtagaacggaatcagtggtgac
cgtggtatccaccgccaccgccgccgccgccgtaggtgggcgtgggaggg
acggggtggtagtccgggtggggctgcggcttgggttccggcttgggcac
cgggccgtagtctgggtggggctggggcttgggctccggcttcggtggca
cgggcttcttgtggtggaagtgctccatgatgtgtttcttgatgggcccg
cacagggtcatggactggcactccggcgacgctgcagacggcgtggcaat
gttgtcgccggcgacgatgccgaaggtggtgccctcgg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: CB881957
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||