Fusarium head blight (FHB) is the most serious disease in decades in the malting barley crop of the upper Midwest. To develop FHB resistant barley germplasm we are creating transgenic plants carrying antifungal protein (AFP) genes. We have developed or obtained a set AFP genes that we are transforming into barley. We have the following genes in plant transformation plasmids: alfalfa acidic glucanase, Arabidopsis thaliana PR5, rice chitinase, oat thaumatin-like protein 1 (tlp-1), oat thaumatin-like protein 4 (tlp-4), wheat thionin and wheat thaumatin-like protein 1 (tlp-1), barley chitinase, barley Type-I ribosome inactivating protein and barley class-II ß-1,3-glucanase gene. In addition, we are developing plant transformation plasmids with the Fusarium graminearum Tri101 gene. In general, we are focused on AFP genes that have the potential to degrade or disrupt the fungal cell well or membrane. We are using two constitutive promoters to drive expression of the AFP genes: the sugarcane Bacilliform badnavirus and the maize ubiquitin promoter. At the present time, we are using particle acceleration to introduce the AFP genes into barley. Transgenic plants have been obtained for four of the possible 11 AFP protein genes. Partial molecular characterization of these plants will be presented.