Wheat microsatellite WMC 25/06/01

wmc(1A1) Plate reference : 1A1
WMC locus number : WMC Length of sequence : 138
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO1. Trimmed sequence. No Microsatellite No substantial microsatellite. No substantial microsatellite.
PCR Comments :



Length of sequence : 138  Consensus sequence is :

..

TTCCTCGAGG CGCGTGCTGC GTCGGCGGGC CACTGTGGCG CCCACGTCAC 
TGGCATGGAT TTGGGGATGG AGACGTGGGC TACGGCGGCG GCATATTCCG 
GCGTCCACCT CGCCGTGTTG TTGGCGTTTC ATGTCGAC

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wmc1(1B1) Plate reference : 1B1
WMC locus number : WMC1 Length of sequence : 406
Last updated : Mar 22 DŽc 1998 at 16:35
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO2. (CT)(CA) compound. (CA)14 334 to 361. Primers redesigned 19.6.97 to avoid linkers (old primers deleted from database 19.6.97). Bases 244 to 334 show homology to 2h1.2 bases 27 to 117 (91 in longest run, maxScore = 67). True duplicate with 2h1.2, 1b1 has priority.
PCR Comments :
Forward primer details :
AcTgggTgTTTgcTcgTTgA at 230 length 20 bases, Tm 64
Reverse primer details :
cAATgcTTAAgcgcTcTgTg at 358 length 20 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 148 bases.



Length of sequence : 406  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACATGG AGCCCACGAT ATCAGATTTG 
TTTAGATAAT GCAAGAGGAT ATCTCCCTCG TAAGTGTTGA TCTAATTTTC 
AGCACAAACT GCTAAGTGCT AACATTCACC TGTAAGTCTT GAAATACATA 
TTTCAGAGAG AAGTTTATCA ATTGCCCTTT AAGGCTAGAC ATTAGTTGCT 
AAAGGTGCAC ATGACCTCAT GAGTTTCTAA CTGGGTGTTT GCTCGTTGAC 
AATGCAATAG TGATTCGCTT TACTTAACCA ACTTTGTGTT GAAATATATA 
GCATAATCTC TCTCTCTCTC TCTCTCTCTC TCTCACACAC ACACACACAC 
ACACACACAC AGAGCGCTTA AGCATTGTCT GATAGTCCAC GCGCCTCGAG 
GGATCC

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wmc(1C1) Plate reference : 1C1
WMC locus number : WMC Length of sequence : 110
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO3. No obvious microsatellite.
PCR Comments :



Length of sequence : 110  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG GGGTTGGACG GCCCAGGCGC CTCATCATCA 
CTAT1GTCGA GGATGATGAC ACCGCCCTTG TCGTGCTAGT CCACGCGCCT 
CGAGGGATCC

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wmc(1D1) Plate reference : 1D1
WMC locus number : WMC Length of sequence : 205
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO4. Trimmed to Rsa fragment. Not enough room for primers. Complex GT microsatellite. Core is similar to 11h5, though flank is different. Bases 17 to 48 show homology to 21c11 bases 91 to 122 (complement) (32 in longest run, maxScore = 20).
PCR Comments :



Length of sequence : 205  Consensus sequence is :

..

GTACGTGTAG TGTGTGTGTG TGTGTTTGTG TGTGTCTGTG TGTATGTGCG 
TGTGTGTAGT GTGTCTGTGT GTATGTGTGT GTGTGTGTGT ATGTGTGTGT 
GTGTAGTGTC TGTGTATGTG TATGTGTGTG TGTGTGTGTA TGTGTGTGTG 
GAGCGGGCCG GGCGCGTACG GGCGGCGGGG CCGGGGACGG CGACGAGACG 
GGTAC

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wmc(1E1) Plate reference : 1E1
WMC locus number : WMC Length of sequence : 377
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO5. No obvious microsatellite
PCR Comments :



Length of sequence : 377  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACTGCT CGAGGTGCAG GAGAGGGGGC 
CATCGGGGAG GAGCTCATCG GGAACAACCG CGTCGAGGAG GAGATCGAGG 
TCGACGTGGC GTTGCGGAGG CGACGGTGGT GGCGGCAGTC AGATCCGTGG 
CAGAGAAGGG AAAGAAGAGA CGACGCGGCG GATCAGGGCT GGTAGGGGCG 
CGGCGGGGTG GTGGCGCGCG GCGGGCCATG GCGATGTGGG AGAGGAAGAT 
TGCGGACAGA AGAGTGGGGG AGATTTTCTC CGGCGGCGGC GAACGCTTTG 
AGGCACGGGT GAAGGAGACC TAATTTAGTC GAAATTTTTG GCACTGCGCG 
CCCTAGTCCA CGCGCCTCGA GGGATCC

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wmc(1F1) Plate reference : 1F1
WMC locus number : WMC Length of sequence : 187
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO6. GT(GA) microsatellite. Not enough room for primers.
PCR Comments :



Length of sequence : 187  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAGGGGGG GGTGGGTGTG CGTGCGTGTG 
TGAGAGAGAG AGAGAGAGAG ATGGAGTCTT GGTGTGTGTG TGGGTGTGTG 
TGTGTGCATG CGTGCGTGTG TGTGTGTGTC GCGGGGTCCT CTGTGGCGGA 
TGTAATTTAA GTGTAGTCCA CGCGCCTCGA GGGATCC

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wmc(1G1) Plate reference : 1G1
WMC locus number : WMC Length of sequence : 251
Last updated : Lun 2 Nov 1998 at 9:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO7. Trimmed to Rsa1 fragment. (CT)(CA) compound. Not enough room for primers. Member of 1G1, 1G3, 1H8, 1F12, 2F8 2D11, 2H11, 2A12, 3a4, 3a5, 3g6, 3H6, 3D7, 4a2, 4c4, 4b7, 4b8(2), 4g9, 4c12, 5b3, 5D3, 5g5, 5g6, 5h10, 5d12, 5H10, 6A4, 6G1, 6H3, 6H4, 6B11, 6F9,6F11,6c12 , 7a10, 7e3, 7H3, 7h5, 7b6, 8a11, 8b4, 8D11, 9H3, 11d4, 11f9, 13G1(2), 13F4, 13F5, 13B6, 17e10, 17f10. Sequence 11d11, 11D7 are related to this family but are slightly different Bases 96 to 142 show homology to 21h2 bases 6 to 52 (complement) (47 in longest run, maxScore = 43).
PCR Comments :



Length of sequence : 251  Consensus sequence is :

..

GTACACACAC ACACACACAC GCACACTCCC CCCCACACAC ACTAAGACTC 
CATCTCTCTC TCTCTCTCAC ACACACACAC ACCACACACA CACTAAGACT 
CCATCTCACA CACACATGCT CTAGGCGGAG CATTTGTTCC TACCACCCTT 
CATCCAACCT TACCCGTGTG ATAAACAATC ACCTTTATTT ACTTGCATAA 
CATGGAAAAA TTCCACTTTA CTTTAGTAGT CAGCAAACTT ATCATCTGTA 
C

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wmc(1H1) Plate reference : 1H1
WMC locus number : WMC Length of sequence : 498
Last updated : Tue, Aug 5, 1997 at 3:35 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGRO8. GT mcrosatellite. Primers as orginally designed overlapped with vector. Do not use. No room for primers. Similar but npot identical to 2g9.
PCR Comments :
Expected size of amplified fragment (Chinese Spring=) 231 bases.



Length of sequence : 498  Consensus sequence is :

..

AGGCGCGTGG ACTAACCGTC CAAATGCACC TGCCCCAAGG CAGACCTATG 
CTGCACCTCG TCTTCCTCCT CCACCATCTA GGCAGCCAAG ACTTCCAGCT 
CCACCACCCG GAGCTCTTGT TCCCACTCCC AATAATGGTC TGTGCTACAA 
GTGTGGTTAA CCAGGTCACC TTGCTAGGAA CTGCTATCAG AATCAGAACC 
AGAATCAACT GGCCCTTCCA GCAGCTGGCC GTGGAAACAA CCAGCCCCGC 
AACAGCAATG CCAAGTCTTA TGGTCGTGTT CATGCCAACC ACGTTGATCT 
CAGTGAAGCT CAAGACCAGC CTGCTACTGT GATGGGTGGT TGCTTGTATT 
TATGTGTGTT TGTGTGTGTG ATTAATTGTT TTGTTTTGAT TGGATATTTG 
AGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGATTAATTG CACGTGCAAA TACATAGTCC ACGCGCCTCG AGGGATCC

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wmc(1A2) Plate reference : 1A2
WMC locus number : WMC Length of sequence : 256
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR09. (GCC)5 microsatellite, too small for now.
PCR Comments :



Length of sequence : 256  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACACGA CTCGGCAGCT CATGTCCAGT 
GGGAACAGCG ACATCGGCAT GTGTGTGGCG AGAAGCCTCC GGGAAGAAAG 
AGTCTCAATG TGTGCATGAT GATCCGCGCC GCCGCCGCCG CCAACATGAG 
GGATCTCGAT GAATGGCACG ACTCGCTGAG GGTGGCTAGT GAGGAGGGGG 
TGGTCGTCGA AGCTGTAGCG AGAGCGGTTG ACTAGTCCAC GCGCCTCGAG 
GGATCC

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wmc(1B2) Plate reference : 1B2
WMC locus number : WMC Length of sequence : 378
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR10. from 2 forward 50 reverse. No obvious microsatellite Bases 221 to 235 show homology to 25b9 bases 86 to 100 (15 in longest run, maxScore = 19).
PCR Comments :



Length of sequence : 378  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACAATA CAATGTGCTT CCTCTCTTTT 
CCCTGTTACG GCTCAGATCC TACACTACGA CATCCATCAA TCTAACAAAG 
AACCATCATA TCTATCAACA AATCATCTAC CGCATGGATC AAATAAAATT 
TCTGTATTAG ATCTGTGCCC GGAGGGAGAT GCATGCGGTT TGGGAAGGGA 
AGAAGATTCG CGAGGGGGAT GAGGGGAGAT CTCACCTACT TACCGGAGTT 
GGTGGCGGCC GCGCAGATCA GGCGGCGAAG AGATGCGTTG TCGGGAGCTG 
GCGGCGGCGG CGCTAGTCGA GAAGGGGAGG AAGAAGTTGA AAATGATGTG 
GAGCTAGTCC ACGCGCCTCG AGGGATCC

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wmc(1C2) Plate reference : 1C2
WMC locus number : WMC Length of sequence : 988
Last updated : Lun 9 oct 2000 at 14:43
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR11. no overlap between forward and reverse sequences. CA and CAA microsatellites.571+417 bases=988. Contains multiple inserts (2). No room for primers. TTgcTgAcAcAAgAgccAccAc (57, tm68) and AgcAAggTcAATcgAgAcATAc (337, tm60) were originally selected as primers, however these are either side of linker.In the reverse sequence in the last 100 bases before the vector there is 50bases of homology to 6G4 (first 50 bases). Bases 305 to 354 show homology to 19b2 bases 258 to 307 (50 in longest run, maxScore = 33). Bases 783 to 805 show homology to 19h4 bases 78 to 100 (complement) (23 in longest run, maxScore = 20). Bases 856 to 892 show homology to 10g8 bases 4 to 40 (37 in longest run, maxScore = 24). Bases 332 to 354 show homology to 2b6 bases 160 to 182 (complement) (23 in longest run, maxScore = 40). Bases 384 to 427 show homology to 25g8 bases 154 to 197 (44 in longest run, maxScore = 58). Bases 680 to 720 show homology to 24f8 bases 58 to 98 (41 in longest run, maxScore = 47). Bases 336 to 370 show homology to 26b1.2 bases 168 to 202 (35 in longest run, maxScore = 63). Bases 295 to 357 show homology to TW2f09.1 bases 76 to 138 (63 in longest run, maxScore = 63).
PCR Comments :



Length of sequence : 988  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACCGAG AGGCCCGAGG TCATCTGCCG 
TGCCAGTTGC TGACACAAGA GCCACCACCT CAGCACCAGC CACAGCCTCA 
ATTCCACCAG CTCCAGCATC TACTTCAGCT CCAACTCTAG CGTCTACTTC 
AGCATCTACC GAAGCCTTCG GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT 
GTTGTCCCTG TTGTTGTTGT CATTGTTGTT GTTGTCGTTG TTGTTGTTGT 
TGTTGTTGTT GTTGTAGTCC ACGCGTGGAC TAGGGCCACG CTCACTCTCT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTTTGTAT GTCTCGATTG 
ACCTTGCTCT TTCTCGGACA AAAATGATAT ATCACCATGT TTGAGCCCAA 
TTCGACCTAT TCACATTGTA TACTCTCTCA CGCCACATTG TCTATCTAGG 
TCACTCTCTC CAACCCGTCT CACTCTTTCG ATAGCACGGC GACCCTATGT 
GATCGATTGA CCTTGCTTCC TCCCCACGCA CAAAAATATA TCTACACGTY 
CTGTGATTGG ATCAACTCTC A
(break)
GTGAAAACAA TCCT1GAATA 
TTACTCAACC 2TTTTTTCCT GTGAAAGTT1 TTGACGGTGC AATATTTCCA 
TATATTTG3A TTTAG1TCCA GTTTG2GTTT ATTTGGGTTT GGACGTTTTA 
GGTGCGCCCT AGTTTTTTTA 2TACTGATTT TGTGTGTGTG ACTGAGAGAG 
AACGTGTGTA TGTGTCCATA TGTGTGTTGT GGCGGAAGGG TAATGTGGAG 
ACGGTGTGTG TGTGATATAG ACATAGAGAG GTGTGRATGT GCAAATGATC 
ATGCATGAGT GAGACATAGA CAGATGCCGA TACGTTGTGT ATACATGAGA 
GGACGGTTTT TTAGTTTACT TTGTGTGTGT GTGTGCGTGT GTGTGTGTAT 
GTGAGTTTGA GCATGTTGTG AAGTAGTCCA CGCGCCTCGA GGGATCC

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wmc2(1D2) Plate reference : 1D2
WMC locus number : WMC2 Length of sequence : 295
Last updated : Wed, Aug 6, 1997 at 9:16 am
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR12. Trimmed to Rsa1 fragment. (CA)46 microsatellite. (Near) duplicate of 1G4, 1d12. Use 1G4 primers. ATCTTAAG TAATGCTCCC AG (53. tm 53) and AgcAcccTcTATTgTcTATg (253, tm 54) originally selected as primers.
PCR Comments :
Forward primer details :
at length bases, Tm
Expected size of amplified fragment (Chinese Spring=) 270 bases.



Length of sequence : 295  Consensus sequence is :

..

GTACGAAGAG ATCCGTTCTG AGGTGAGTTA ATTCAATTGG ACACAAAAAA 
TTATCTTAAG TAATGCTCCC AGGAGAGAGT CGGAGAGTTC AACATTACGT 
TGATGGATGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GATAGTAGRA ATACCACACT ACCGTAGTAG TATAGCA1TA 1TACACTAAT 
RACATAGACA ATAGAGGGTG CTCCCCGC8T CCCTGGATCC GGTAC

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wmc(1E2) Plate reference : 1E2
WMC locus number : WMC Length of sequence : 210
Last updated : Wed, Aug 20, 1997 at 1:10 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR13. CCA and CA microsatellite. Trimmed to RSA1 fragment. Not enough room for primers. Bases 97-210 match part of 4G12
PCR Comments :



Length of sequence : 210  Consensus sequence is :

..

GTACACCACC ACCACCACCC CACACACACA CTAAGACTCC ATCTCTCTCA 
CACACACACA CAAACACACA CACACACACC TACAGGCAAC CAATAAATGG 
CACAAGTTAA ACTGCAATTG CATAAATGAT GTGAAATACT GTAAGACATG 
GGATGCAGCT AACCTTGATA GAAAACAACA ACATCGGCCG TTATGACCCT 
GCGTAAGTAC

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wmc(1F2) Plate reference : 1F2
WMC locus number : WMC Length of sequence : 0
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR14. No insert.
PCR Comments :



Length of sequence : 0  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC

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wmc3(1G2) Plate reference : 1G2
WMC locus number : WMC3 Length of sequence : 744
Last updated : Lun 2 Nov 1998 at 10:02
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR15. (CT)(CA) compound microsatellite. Multiple inserts (2). Originally AATAAcATAAAcAgATggTgcc (29, tm 57) and TgcTAggTgTTTTAgTcTggTA(293, 56) selected as primers - DO NOT USE OLD PRIMERS (either side of linker). New primers redesigned 19.6.97 for the second insert (NB base 10 of reverse primer is shown as G, this is from a 'most likely' C in the forward sequence). Bases 18 to 56 show homology to 21b5 bases 1 to 39 (39 in longest run, maxScore = 31).
PCR Comments :
Forward primer details :
ATTcAAgTcTcTgcAgAccAcc at 436 length 22 bases, Tm 62
Reverse primer details :
cccTgAgcAgcTTcAcAgATTAc at 678 length 23 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 265 bases.



Length of sequence : 744  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGC GTTTTAAAAA TAACATAAAC AGATGGTGCC 
ATTTACTAGT CCACGCGTGG ACTAGGGAGG AGAGAGAGAG AGATGGAGTC 
T2AGTCTGTG TGTGTGTGTG AGAGAGAGAG AGAGAGATGG AGTCTAAGTG 
TGTGTGTGGG GGGTGCGTGT GTGTGTGTGT GTGTGTGTGT RTGTGTRTGT 
GTGTGTGTGA GAGAGAGAGA GAGATGGAGT CTTAGTGTGT GTGGGCGGGG 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTAG TCCACGCGTG GACTACCAGA 
CTAAAACACC TAGCAAAGGA AAAAAGCCCA GCACCAACAC ACGCTCATCC 
CCTTTATTTC ATGTTGCTCC ATCGACAAAA TCTAATCGAT GTATTACGTA 
GATTGCAGTC TCTTGCGACC TATCTACCTA TGGTCTATTC AAGTCTCTGC 
AGACCACCGC CAGGATAAAT ATTTTCCGGA CTCCCAGCGA AGATCGGAAG 
GGGA1TGCTT TCCCCAGGGA TGRATCCAAA GGGGGTTTAC CCACTAACAA 
ATATATCCCT CGTTGATAGC GA2TAGGCGG ATCCTAATTA TGCGGACGGT 
TTTAATTGAT TCACA1GTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTCA 
CAAAGTGAAG 4TG1TTTGTT TTTTTAGGTA ATCTGTGAAG CTGCTCAGGG 
ACACATGCCC CAGCCC4TGT TAGTCCACGC GCCTCGAGGG ATCC

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wmc4(1H2) Plate reference : 1H2
WMC locus number : WMC4 Length of sequence : 913
Last updated : Lun 30 oct 2000 at 16:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR16> No obvious overlap between forward and reverse sequences. AG or AC microsatellite. 439 +474. Bright smeary fragment on agarose after PCR 61C anneal. Bases 216 to 236 show homology to TW3e03.2 bases 184 to 204 (complement) (21 in longest run, maxScore = 43). Bases 131 to 178 show homology to TW1g07 bases 95 to 142 (48 in longest run, maxScore = 79). Bases 211 to 264 show homology to TW5h08.3 bases 4 to 57 (54 in longest run, maxScore = 60). Bases 94 to 123 show homology to TW6d03.1 bases 183 to 212 (30 in longest run, maxScore = 156). Bases 212 to 264 show homology to TW6a06 bases 154 to 206 (53 in longest run, maxScore = 132).
PCR Comments : 61C anneal, smear;
Forward primer details :
TAgAAAgggggTTcggTTggTTgg at 262 length 24 bases, Tm 71
Reverse primer details :
AgccTcTTgggcTgATggATgATg at 412 length 24 bases, Tm 71
Expected size of amplified fragment (Chinese Spring=) 174 bases.



Length of sequence : 913  Consensus sequence is :

..

GCGCCTGGAC TAACCGAGGC ATC7GGCAAA GGTTGCCATT TCACTGC7AC 
GAGACCAA8A CCAGGAGGAC TACCAGACGG AGGTCACCGT GGGGCCCAAG 
ACGGCGTCAT CACCAGAGCT TTGTGCAGGC GAAGACTACT TTTGTCAGGA 
TA4CTGATAC TTGCTGTCCC CCTTCAAATT AGCCCGCCAA TGTTGGCTCC 
CTTCCCGCTT GATATTTGGG AAGAGGACCA GGGCCTCTAT AAATAGGACC 
AGCCACCCAC ATAGAAAGGG GGTTCGGTTG GTTGGAGGAA 8CA8ACA8A8 
A8A8A8A8A8 A8A8A8A8A8 A8A8A8A8A8 AAGGGTGACT GA7CTCCTCC 
GAGCA8TCCA TCGCCCTATC CAAGAACA8A CCCTCGCGAG GCTGTTCTTC 
CTTGTATTGT TCATCATCCA TCAGCCCAAG AGGCTATCC
(break) 
AACGGTGGCC CGGACCAGTA TTAGTCCA1G 1GTGGA1TA1 TGG2GTGTGC 
ATGCA2G1GT GCGTGTGTGA GTGTTGCGCG TGTGCGTGTG TGGCTGCGTG 
TATATGTGTG AGTGTTGCAT GTGTG1GTGG GTGCA2GTGT TTGTGTGAGT 
GTTGCGTGCA TGCAGTT1GT GTGCA2GTGT GTGTGTGTGT GTATAAT1AC 
CACACCAAAT 1ATGTGTGTT 7AAATAGACG G1T1AGCATA CC7ATACAAG 
GCCACCTTGT CCGTTGTGCC CGCGGTCA7G A1TCCGRACG ACCATCC1AT 
ATTTTTTTGT 1GTTTGTTTT CATTTTTA1T AGCAAGATTC CCGTGCGTTG 
CA1GTAA1AT CAAGATGCAT TTGTATGA1T AGTTTATTTT GTGAGAGAAA 
AGTATGAACG AGAGAAGGCC TTATTTGCAA ATGTGGAGAG GTGTGTGGGT 
TAGTCCACGC GCCTCGAGGG ATCC

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wmc(1A3) Plate reference : 1A3
WMC locus number : WMC Length of sequence : 103
Last updated : Thu, Aug 21, 1997 at 2:26 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR17. No obvious microsatellite. Matches first insert in 4h10, and 5e1, 5B4, 5d4.
PCR Comments :



Length of sequence : 103  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACACCG ACCGATCTCC CGCTATACAT 
ATAGCTAGCT AGGTCCTTAT AACTCATTTT CCCCCACGCG CCTCGAGGGA 
TCC

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wmc5(1B3) Plate reference : 1B3
WMC locus number : WMC5 Length of sequence : 384
Last updated : Wed, Sep 17, 1997 at 3:16 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR18. Trimmed to Rsa1 fragment. (CT)(CA) compound. Bases 187-290 (ie. 3' side of microsatellite) match part of 9e5. Bases 20-140 ie 5' side of microsatellite match part of 4d6.
PCR Comments : 61C anneal, product; 51C anneal, product;
Forward primer details :
TgTTcTTcTggcATTAgATgggTg at 66 length 24 bases, Tm 66
Reverse primer details :
cTggTTccTcgTcATcgATcTTgg at 343 length 24 bases, Tm 70
Expected size of amplified fragment (Chinese Spring=) 302 bases.



Length of sequence : 384  Consensus sequence is :

..

GTACATCATG TTCAAACCCC CCTTTGCTTC CGACCAAATG TGGACCGTGT 
GCGGGCCCAC AGATTTGTTC TTCTGGCATT AGATGGGTGA GACCACATAC 
GAACCGCACC TGCGGGTGGC CCGATGTGTG TGTGTGTGTG AGAGAGAGAG 
AGAGAGAGAG AGAGTTTGTG TGAAAATGAT GTGGTGTGTG ATATTTAAAT 
AACCAAATCA CAAATTTGAT GTGGCACATG TGCATCGCAA ATTAACCGGA 
CATCCCTGAC CCCACGGAGG TTCATCTAGT GCGAAGTAGT GAGCTGTGCA 
AGGCTGTAAC TGAACATGTT GGCACCTTCA TTGAAGAAGT TGCCAAGATC 
GATGACGAGG AACCAGATAT TGATCCTTAC GTAC

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wmc6(1C3) Plate reference : 1C3
WMC locus number : WMC6 Length of sequence : 580
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR19. Trimmed to Rsa1 fragment. (CA)10 microsatellite.
PCR Comments : 61C anneal, product;
Forward primer details :
cccggTTTATAgATAAAgcgcccA at 130 length 24 bases, Tm 69
Reverse primer details :
AccTcAggAcgAcTcgTcgAcTcA at 200 length 24 bases, Tm 71
Expected size of amplified fragment (Chinese Spring=) 94 bases.



Length of sequence : 580  Consensus sequence is :

..

GTACATTAAA ATATGTGGAT TACAACTGTG CTTTTGTCCT AACATCACGT 
TTCATAAGTT CTGCCGAAGT GTGCCTGGGA GCTAAAATTA GCATCTGATA 
ATGTCAGTAT TAACGAAAAC GGCTTTTGCC CCGGTTTATA GATAAAGCGC 
CCAACCAAGC CACACACACA CACACACACA CCAAGGTTTA CGAGTCAGAT 
GAGTCGACGA GTCGTCCTGA GGTTGAGACT CGTAGACTAA GCGTGAGTAG 
TCGACACTAA AGTTGCGACT CATAGACTAG TCGACACTGA AGTGTAGTCG 
GCCCTGGAGT TAAGACTCAT AGACTAGTCC TTGGACTCAT AATCCATGAC 
ACACACACAC ACCAACCCTG GTGATGATTT CAGCTATACA AGAGAAGCAC 
AAATAAGGCC AAACAAGAGA CCAGTAAACC GGAACTAAGC AAAGCTAAGC 
TAAGCACATG GACAAAAGGT GCTTGCTTGA TCCTGACGAC AACCGTAAAT 
CTTCCTAGAG TGCCTCGACA CGCAACGCCC AGCGGGACAC CACCCCCAAG 
GTGCACACCT AGTGACAGG8 CAGGTGGTAC

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wmc(1D3) Plate reference : 1D3
WMC locus number : WMC Length of sequence : 272
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR20. No obvious microsatellite
PCR Comments :



Length of sequence : 272  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACAATG CAGGCATTAG AACATTGTCT 
CGTATTAAAC ATGCTACAGA AAAGAGAGGA GAATATAACA AACCTTGACA 
CGTGCATTAG GCACGGGAAA TGTGCATCTC ATAAAGAGAT CCAATGTTCC 
AGCAGGAACC AGGCGCTCCC CCTTCCTAAC TCTGCCATTC AGTAGCATAG 
CCCGAGCTTT GGAGGCATTG GGGGTAGACC TGGGACTGTT AGTCCAC4TA 
GTCCACGCGC CTCGAGGGAT CC

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wmc7(1E3) Plate reference : 1E3
WMC locus number : WMC7 Length of sequence : 610
Last updated : Wed, Aug 27, 1997 at 3:54 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR21. Trimmed to Rsa1 fragment. Multple inserts (2). Complex (C)(CA)(GA) repeat in second fragment. Last insert highly homologous to 1B5. Bases 1 to 32 show homology to 19a6 bases 133 to 164 (32 in longest run, maxScore = 27).
PCR Comments : 51C anneal, product;
Forward primer details :
gcTTggAcTAgcTAgAgTATcAT at 356 length 23 bases, Tm 56
Reverse primer details :
AcAATcTTcAATTcTgTcgcAcg at 536 length 23 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 203 bases.



Length of sequence : 610  Consensus sequence is :

..

GTACTCGCAA GCAAGGAGCT ACACTACATA TGTATGCATT GGTATCAAAT 
GGAATAAGGG TATCATATGT GGATTGAACT GCAGAAAGCC GGAATAAGAG 
GGGGGTAGCT CGTCCTATCG AAGACTACGC TTCTGGCCAC CTCCATCTTG 
CAGCAGAAGA AGAGAGTAGA GGGTAAGTTC ACCAAGTAGC ATCGCATAGC 
ATAATCCTAA CCGATGATCC TCCCCTCGTC GCCCTGTGAG AGAGCGATCA 
TCGGTTGTAT CTGGCACTTG GAAGGGTGTG TTTTATTAAG TATCCGGTTC 
TAGTTGTCAT AAGGTCAAGG TTAGTCCACG CGTGGACTAG CACCCAAGAT 
ATCTAGCTTG GACTAGCTAG AGTATCATAC ATAGTGTGCG AGAGAGACCT 
ATAGATGGRG TATATATGCA TG1GAA5TAG AAAGACCCCC CCCCCCACAC 
ACACACACAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG ATGGAGAGAA 
AAGGAGAGGG ACCCACAAGG TTTTTGTGT1 GGATGCGTGC GACAGAATTG 
AAGATTGT1G ACGAGAGAGA GAGAGAGAAC ATGAGTCCGG GAGAGGGGGA 
GGT1TTGTAC

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wmc(1F3) Plate reference : 1F3
WMC locus number : WMC Length of sequence : 155
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR22. Trimmed to Rsa1 fragment. Short CCG microsatellite too short for now.
PCR Comments :



Length of sequence : 155  Consensus sequence is :

..

GTACGACGCC GCCCACACCG ACGCCCACAC CGCCGCCGCC GCCAAGCCCG 
ACTGATGATG CCGCCGAGAC TACCCGCAGC ATAGAAGCCA CGCCGACGCC 
ACCCAGGCCG ACCTCGCCGT CTGATGCGCG CATGCGTCCT TTCTGCTTTT 
TGTAC

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wmc(1G3) Plate reference : 1G3
WMC locus number : WMC Length of sequence : 203
Last updated : Tue, Aug 5, 1997 at 3:21 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR23. Both( CT)(CA) and (C)(CA) microsatellites. No room for primers. Member of 1G1, 1G3, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 5H10 9H3, 4b7, 4g9, 4c12, 11f9.
PCR Comments :



Length of sequence : 203  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAGTGGTA GGAACAAATG CTCTGCCTAX 
AGCATGTGTG TGTGAGATGG AGTCTTAGTG TGTGTGTGTT GTGTGTGTGT 
GAGAGAGAGA GAGAGAGAGA GATGGAGTCT TAGTGTGTGT GTGTGTGGGG 
GGGGGGGGGT GCGTGCGCGC GCGCGCGTGT AGTCCACGCG CCTCGAGGGA 
TCC

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wmc(1H3) Plate reference : 1H3
WMC locus number : WMC Length of sequence : 408
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR24. No real microsatellite many short repeats based around GCC. Bases 259-376 match part of 9C1 (ie 3' side of GCC repeat). Bases 116 to 130 show homology to 17e9 bases 1 to 15 (15 in longest run, maxScore = 24).
PCR Comments :



Length of sequence : 408  Consensus sequence is :

..

GAATTCCTCG AGG1GC8TGG ACTAACATCG TAAATAACCA ATTTTCATAT 
AGAAAGACAT ATCACACGTA TTTGTATACG TTTATATTTG TATTATGATC 
ATCTATATAA AGAGACGTGA GGCTGGATGT TAGCCACCCG CGCAAAACCC 
TAAACCTACC TCCTCAACTT GGTATCAGTC AGGTCGGGCC GCCGCCGCAC 
GTCCCTGGCG CCGCAGCCCG ATCCCATCTT CTGCGCCGCC GCCGCTGCTT 
GCTAGCTAGC TTCCGCGTAG CCGCCGCCGC TTGCTAGCTA GCTACGCACA 
TACGCAGGAT CCATCCAGCT GCTTGCTAGC TAGCCACAGG GCCGCCGTGC 
GAACACTACG CGCCGCCCGA TCCATCCGCC GCCCTAGTCC ACGCGCCTCG 
AGGGATCC

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wmc8(1A4) Plate reference : 1A4
WMC locus number : WMC8 Length of sequence : 321
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR25. CA microsatellite, one main and several small, few repeats also using GCAC theme. Trimmed to Rsa1 fragment.
PCR Comments : 61C anneal, polymorphic on acrylamide;
Forward primer details :
cAcgcgcAcATcTcgccAAcTAA at 115 length 23 bases, Tm 73
Reverse primer details :
cgTggTcTAgTccgcgTTgggTc at 214 length 23 bases, Tm 72
Expected size of amplified fragment (Chinese Spring=) 122 bases.



Length of sequence : 321  Consensus sequence is :

..

GTACCAGA4G CCAGCCCACT CACCCTCTTG CCACGCCCGT GCTCCACGGT 
TCACGCGGCC GCTGTGCACA CACGCACACA AACGCGACCG GGTCCAGCGC 
CCAGCACGAT CATGCACGCG CACATCTCGC CAACTAACCC ACACACACAC 
GCACATACAT GTGGTCACAC ACACACACAC ACACACACAC ACACACACAC 
ACACACGGCT ACGGACCCAA CGCGGACTAG ACCACGTCCG GCACGTCTGG 
CGCACGGACG CACGCGCACC CGACGCGCCC TGGGCATAGC CGTGGCTAAT 
TTGGCTATCA TTCACTAGTA C

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wmc9(1B4) Plate reference : 1B4
WMC locus number : WMC9 Length of sequence : 317
Last updated : Thu, Aug 21, 1997 at 11:19 am
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
PIC Value : 0.69
Comments : AGR26. Trimmed to Rsa1 fragment. CA microsatellite. Expect Fragment 168 bases. Polymorphic on agarose. (2 or 3 polymorphic loci detected?). Bases 8-175 ie. 5' side of microsatellite match 5g10, bases 114-220 (ie. 3' side of microsatellite) match 5b12, also 5F2. Bases 156 to 210 show homology to 12d4 bases 80 to 134 (55 in longest run, maxScore = 51).
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
AAcTAgTcAAATAgTcgTgTccg at 75 length 23 bases, Tm 59
Reverse primer details :
gTcAAgTcATcTgAcTTAAcccg at 220 length 23 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 168 bases.



Length of sequence : 317  Consensus sequence is :

..

GTACCGYGGG TCGTGGATGA CACGGAAGTT CCCCGGATAT TGTGGGTAAA 
GTGTGCAACC TCTACGGAGT GTAAAACTAG TCAAATAGTC GTGTCCGCGG 
TCAAGGACAG ATGGGCGGTG CTGCTTAAAC TACATCCAGA GCTTTACAAA 
CCACGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGT2GAT TAAGCCAATC GGGTTAAGTC AGATGACTTG ACACGATGAT 
CAAGTTGGAT 7GGTGTCCRA CTCTGCTTAT GTTGATAT5T AC5ACTTGTT 
CATACGGATA TCTGTAC

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wmc(1C4) Plate reference : 1C4
WMC locus number : WMC Length of sequence : 371
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR27(CA) microsatellite. No room for primers. Matches 8a12, 3f6 ,11c7 related to 5F11. Bases 239 to 279 show homology to 21c10 bases 68 to 108 (41 in longest run, maxScore = 80).
PCR Comments :



Length of sequence : 371  Consensus sequence is :

..

GAATTCCTYG AGGGG8GTGG ARTA2GCATA GGCGGATCAT TGACATGAGG 
AAGTGCCTCA TTACCAAAAT CGACGAGCCC AGATCAAGCC ACAAGCAGAG 
CAAGCGTCAC AAGAAGTAGA TGATGATCAG ATGATCGTTT ATTCTAGTTA 
ATCATGCATA TAATATATAG TTTACTTTAT TGGTGTGTGG AAATGTCATG 
TGTGTAGTAG CCACCTATAT AATTATGCAT GTAATAGTTT ACTTTGGTGT 
GTGCAAATGC CATGGTTGTA GTAGCCACCT ATGCAAGTGG ATGTTTAATT 
TGTTATGCAA GCATGTCCTT ATAAGTGTGT GTGTGTGTGT GTGTGTGTAG 
TCCACGCGCC TCGAGGGATC C

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wmc(1D4) Plate reference : 1D4
WMC locus number : WMC Length of sequence : 194
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR28. No obvious microsatellite
PCR Comments :



Length of sequence : 194  Consensus sequence is :

..

GAATTCCTCG AGG1GCGTCG GCCTCGATCT GTTCTTTTTG GCGCCGACCG 
GTCCAACCGG CCCAGCCAGT GCCATGCCTC CCTCTGCTTC CAATCGGGCC 
CCAGCCCATG GGTGAGCAGC CCCCAACAGC CCCTCTCTTG TGCATGTTTG 
GCCCAGCAAG TTTCGGCCTG TAGTCCACGC GCCTCGAGGG ATCC

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wmc(1E4) Plate reference : 1E4
WMC locus number : WMC Length of sequence : 473
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR29. No overlap between forward and reverse sequences. (CA) motif common, and AGCn. Difficult to get primers. Related to 6A7
PCR Comments :



Length of sequence : 473  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAGCCGGG AGAGGGCTGC GCCTCAGCCG 
GACGCCCCTG CAGCAGCAGC AGCAGCACCA CGGCTCCTAC CACCACCATG 
AGACGTGAGA TGCCAATATG TGGCCTAATC TGCATCATCA TCGTCATCAT 
CTTCTCTGCT CCAATAGTTG AGTGGAGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTG
(break)
GA1TCCAT1T 1T1TCCCT1T 1T1TCACACA CACACACACA 
AAGTGAGA1T CCATCTCTCT 1TCCT1TCTC TCTCACACAC ACACACACTA 
AGA1TCCATC ACACACACAC ATG1TTTAGG 1GGRGCATTT GTTCCTACCA 
CCCTT1ATCC 1ACCYTACCC GTATGATAAA CAATCACCTT TATTTACTTG 
CATAACATGG ACAAATTCCA TTTTACTTTA GTAGTCAGCA CACTTATCAT 
CTGTTAGTCC ACGCGCCTCG AGGGATCC

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wmc(1F4) Plate reference : 1F4
WMC locus number : WMC Length of sequence : 331
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR30. Complex of (too) short microsatellites.
PCR Comments :



Length of sequence : 331  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACCAGT CGCGAACACA CACACACTGC 
CATCGCGAAC AAGGTCTCAC ACACAAGCAC ACACACAGAT AAGTTCAGTT 
GTGGGCACAA CACAACAAGC CCAACACACA CGCACACACA AACAAATGGC 
GGTGGCGGCG GCGACGCAGG GAAGATCTAA TCCGGCTTTG GAGGTGGTGG 
GGGAAGCGGC GGAGCCAACC GGAGAGCCAT CGCGCGAAGC TGGGTGATGA 
TGGAGGTGCT GGCGTCTCTC TTCCTGGGGT GGCTAAGCGG CCGCCAAAGC 
TGCAAGTTAG TCCACGCGCC TCGAGGGATC C

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wmc10(1G4) Plate reference : 1G4
WMC locus number : WMC10 Length of sequence : 298
Last updated : Wed, Aug 6, 1997 at 9:16 am
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR31. CA microsatellite. Trimmed to Rsa1 fragment. Expected fragment 267 bases. (Near) duplicate of 1D2, 1d12. Polymorphic on agarose after PCR 61C anneal. (2 loci?) - is one of these the same as 1d12. NB forward primer would not perfectly match 1d12 sequence.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
gATccgTTcTgAggTgAgTT at 10 length 20 bases, Tm 59
Reverse primer details :
ggcAgcAcccTcTATTgTcT at 257 length 20 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 267 bases.



Length of sequence : 298  Consensus sequence is :

..

GTACGAAGAG ATCCGTTCTG AGGTGAGTTA ATTCAATTGG ACACAAAAAA 
TTATCTTAAG TAATGCTCCC AGGAGAGAGT CGGAGAGTTC AACATTACGT 
TGATGGATGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GATAGTAGRA ATACCACA1T ACCGTAGTAG TATAGCA1TA 1TACACTAAT 
A5A5ATAGAC AATAGAGGGT GCTGCCCCGC CTGC8CTGGA TCCGGTAC

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wmc11(1H4) Plate reference : 1H4
WMC locus number : WMC11 Length of sequence : 321
Last updated : Thu, Jun 11, 1998 at 11:26 am
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
PIC Value : 0.62
Comments : AGR32. CT microstellite. Expected fragment 177 bases. Products obtained after PCR 61C anneal. (2loci?).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TTgTgATccTggTTgTgTTgTgA at 95 length 23 bases, Tm 65
Reverse primer details :
cAcccAgccgTTATATATgTTgA at 249 length 23 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 177 bases.



Length of sequence : 321  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAGCAGCA AATCAAATTG CCATGCGCTC 
TCAGCACCAC TCACTAGACT GAGTAGTTGC GGGTATATGA TATTTTGTGA 
TCCTGGTTGT GTTGTGATAA TCATGCACAT TTTTGTTCTA CAACAATTAC 
ATACTCCTTT TTGCTTTATT TCCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTCTAGAGTC ATCAGGAGAG ATACTATCGT AGAGTAATCA TTTACCTATC 
AACATATATA ACGGCTGGGT GTATATTTGT CATTGTGATA CATCAGTTAG 
TCCACGCGCC TCGAGGGATC C

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wmc12(1A5) Plate reference : 1A5
WMC locus number : WMC12 Length of sequence : 0
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR33. No insert
PCR Comments :



Length of sequence : 0  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC

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wmc13(1B5) Plate reference : 1B5
WMC locus number : WMC13 Length of sequence : 336
Last updated : Mer 14 juin 2000 at 10:35
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR34. Forward sequence is a composite of 2 sequence runs. 2 CT microsatellites. Expected fragment 109 bases. PCR product obtained (smeary on agrose). Sequence is highly homologous to 1E3 (though not identical). Bases 176-309 (ie 3' side of microsatellite) match part of 9F3. Bases 215 to 241 show homology to TW1a10.2 bases 241 to 267 (27 in longest run, maxScore = 55).
PCR Comments : 61C anneal, smear;
Forward primer details :
gTgTcggATgcgcgcgATAgAAT at 80 length 23 bases, Tm 73
Reverse primer details :
ATgcATAAAAcgcgAccTccccT at 166 length 23 bases, Tm 70
Expected size of amplified fragment (Chinese Spring=) 109 bases.



Length of sequence : 336  Consensus sequence is :

..

GTACACACAC ACACAAAGAG AGAGAGAGAG AGAGAGAGAG AGAGATGGAG 
ATAAAGGGAG AGGGACCAAC AAGGTTTTTG TGTCGGATGC GCGCGATAGA 
ATYGAAGATY GTCGGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
ACAGGAGTTC GGGAGAGGGG AGGTCGCGTT TTATGCATCA AAGACTGATA 
TAAACCATGT TCGAATATAA ACTTGCATTC AAATATTTAA ACTGGAGAAC 
ATGTTGTCTG CAATCCACAC ATGAATGGAT ATATCTGTAT ATCAAACAAG 
TTCATTAATT TTACTTTCAA CATGTTCATG GAGTAC

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wmc(1C5) Plate reference : 1C5
WMC locus number : WMC Length of sequence : 331
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR35. (CGG)5 microsatellite. Too small for now.
PCR Comments :



Length of sequence : 331  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTACGCGGC TGCTCGATGC AGACCCGCGG 
GGCGGCTGGA TCGGCGGCGG CGGCGGCTAG AGTTTGCAGA TCAGAGGGCA 
TAAAACAAGA GGAGGAGGAG CATGGAAGTA AGAAGGCGGC TGGCGGATGA 
GAGTGGAGGT CGAAGTGGCG GCAGCCTCGT CGAAATGGCG AACGGGAGCG 
GCGAGCGGGA TCGAGGGGCG TTGGTCACCA CCTGTGTGCG CATGTGCATT 
TTTTTGGAGA AAAAAATGCA CCTTTTTCAA GGCTAGAAAA AGTGTGCCTT 
TGCACACTAG TCCACGCGCC TCGAGGGATC C

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wmc14(1D5) Plate reference : 1D5
WMC locus number : WMC14 Length of sequence : 488
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR36. Trimmed to Rsa1 fragment. ( CT) (CA) microsatellite. Expected fragment size=243. Microsatellite motif identical to 7a4, 8e11, 7f12, 11d10. Sequence matches second insert 6b7 (complement). PCR fragment obtained after 61C anneal.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
AcccgTcAccggTTTATggATg at 3 length 22 bases, Tm 69
Reverse primer details :
TccAcTTcAAgATggAgggcAg at 224 length 22 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 243 bases.



Length of sequence : 488  Consensus sequence is :

..

GTACCCGTCA CCGGTTTATG GATGAGTGCT ATTGGCGCCG CACCTCTTCT 
CCATCTCCTC CTCCCTCCAT TCCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTCTCTCTCT CTGACACACA CACACACACA CACACACACA CACACACACA 
CACACACACA CACACACACA CCGGAGCCTT GCGCCGCCGT TGTGGAGCTC 
ACCTGCGAGC TTGCCGAGAT GCGCTGCCCT CCATCTTGAA GTGGATGAGA 
GGTGAGGACA GAGTGTGGAG AAGGGTGGGG AAGATCCAGG TCGAGCTCGC 
GAGGTTGGAG TAGTTCGCTG GTTCAACCAA TCCCGAACGA TGTTCAAAAC 
GGATGTGGCA TACTCATTTG CTTTTAGATG CGTGCTTTTT AACCGACGGC 
AAAAAAGGTC TCGCCCCGAA CATGGCACGT TCGGCAGTTA TTGTTTCCGA 
AGA5TTAATA CGTATACA5A AATGCTACAT CTACGTAC

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wmc(1E5) Plate reference : 1E5
WMC locus number : WMC Length of sequence : 0
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR37. No insert
PCR Comments :



Length of sequence : 0  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC

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wmc(1F5) Plate reference : 1F5
WMC locus number : WMC Length of sequence : 431
Last updated : Mon, Dec 29, 1997 at 6:01 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR38. No obvious microsatellite (odd repeats of CCG). Bases 225-394 match (weakly part of 14C6 (complement) Bases 304 to 316 show homology to 14c6 bases 220 to 232 (complement) (13 in longest run, maxScore = 32).
PCR Comments :



Length of sequence : 431  Consensus sequence is :

..

GATTCCTCGA GGCGCGTGGA CTAACGCGAG AGCTAGATTC CTCCAGGAAC 
CCTACCGCTC ACCTATCCCC GTCTTTCTTC GAACACACGA AGCTGGAGGG 
CGAAACCGAT CTCGCCGGGA AAGATCCGTC CGCCGCTTGC TCCACTCGTT 
TCCCCCTTGA AGCCGCTCTC GCTGACTCCT TCTCCTGGCC ATAAACGCAG 
CAGAGGGAGA TGAACGAGCT CGAACGCCGC CACCGAAGCA CACCATGTTC 
ACGTCGTCAC TGCCGCCCGC AGAGGACGCC TCGCCGCCGT CCGACCTCAC 
CTACACCTCC GCCACTGACC GCCGCACCCA TCCGTCGCTT CACCTTCGTC 
GGAGACCCGC CGGAGAAGCT CCACCGCCGA CGCCCGACCC GCCGCCGCCA 
CGTCCCCTAG TCCACGCGCC TCGAGGGATC C

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wmc(1G5) Plate reference : 1G5
WMC locus number : WMC Length of sequence : 218
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR39. CA microsatellite. No room for primers. Bases 60-154 match part of 14E5. Bases 89 to 102 show homology to 14e5 bases 84 to 97 (14 in longest run, maxScore = 27). Bases 71 to 195 show homology to 22g3 bases 9 to 133 (125 in longest run, maxScore = 65).
PCR Comments :



Length of sequence : 218  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACAAGA AAATGGCTAT GTTGCATAAA 
ACTGAGTGGT GTGAAGAATA ACTTTTCCTC ACCCAGGATG ACGAATAGTC 
AACCCCTATA TGCGTGTGTG CGTGTGTGTG TATGCGTGTG TGTGTCTGTG 
TGTGTGTGTT AATTATTAAA AATGTGTGTG TGTGTGTGTG TGTGTAGTCC 
ACGCGCCTCG AGGGATCC

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wmc15(1H5) Plate reference : 1H5
WMC locus number : WMC15 Length of sequence : 476
Last updated : Lun 9 oct 2000 at 14:43
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR40. (CT) (CA) compound microsatellite. expected fragment=295. (2 loci?). Bases 223 to 245 show homology to 21e1 bases 121 to 143 (complement) (23 in longest run, maxScore = 25). Bases 1 to 15 show homology to TW2h12 bases 1 to 15 (15 in longest run, maxScore = 20).
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
AgTccgATTcggAcTccTcAAg at 164 length 22 bases, Tm 66
Reverse primer details :
ggAcTAAccgAgggTAgTTcAg at 437 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 295 bases.



Length of sequence : 476  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAGGAGCA AGGGGGAGGC CCGCCAGCCC 
TCATGGGGCG CGCCCCCAAG TAGGATTCCT ACTAGGAATA GGAATGAGGG 
AAGAGGGGGG GAGGAAGGAG AGGGAGAGAG GGAGAAGGAA AGGGAGGGCC 
GCCGCCTCCT CCTAGTCCGA TTCGGACTCC TCAAGGGGGG GGCGCAACCA 
GCCCTAGGGA CCTCTTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCACACA 
CACACACACA AGGCCCATGT TGGCCCATTA GTTCCCCCGG GGTTTTCGAT 
AATCCCCGAC ACTCCGATAT TTATCCGGTG ACCACGGAAC TCATCCGGTG 
TCCGAATAAC ATCGTGCAAT ATATCAATCT TTTATGTCTC GACCATTTCA 
AGACTCCTCG TCATGTCCGT GATCATATCC GGGACTCTGA ACTACCCTCG 
GTTAGTCCAC GCGCCTCGAG GGATCC

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wmc16(1A6) Plate reference : 1A6
WMC locus number : WMC16 Length of sequence : 236
Last updated : Mar 30 mai 2000 at 9:54
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR41. CT microsatellite. Expected fragment size 109. Small smeary products obtained on agarose after PCR 61C anneal. Bases 191 to 211 show homology to TW3d08 bases 52 to 72 (complement) (21 in longest run, maxScore = 33).
PCR Comments : 61C anneal, smear;
Forward primer details :
AccgccTgcATTcTcATcTAcA at 26 length 22 bases, Tm 66
Reverse primer details :
gTggcgccATggTAgAgATTTg at 113 length 22 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 109 bases.



Length of sequence : 236  Consensus sequence is :

..

GAATTTCCTC GAGGCGCGTG GACTAACCGC CTGCATTCTC ATCTACATCC 
TCCCCTCGCT CTCCCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTGTCTCTGT CTCAAATCTC TACCATGGCG CCACCAGTCT ACCCACGCAC 
CGACAAGGAG TCGCCGCCCC CCGAGGACGC TCACTTGATT AGCAGCGACG 
AGGACCCCTA CATAGTCCAC GCGCCTCGAG GGATCC

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wmc(1B6) Plate reference : 1B6
WMC locus number : WMC Length of sequence : 239
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR42. (CGG)5 microsatellite. No room for primers
PCR Comments :



Length of sequence : 239  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACGAGG TGCGCGGCGG CGGCGGCGGA 
GCTCGGGCTC TGGTGCGTCA GAGGAGGAAG ACGATGGAGG GGAAGAGTGA 
CGAAGGCCTA TAGGCCGGGC CTATTTATAC GGGACATCTG ATAAATGGGC 
GCAAGRAACG AGGAGGCTGG AGACATGATT ATCTTGCCGC AGAGACRCCT 
CGATTCT2TG GGATGTAGTC CACGCGCCTC GAGGGATCC

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wmc17(1C6) Plate reference : 1C6
WMC locus number : WMC17 Length of sequence : 211
Last updated : Thu, Jun 11, 1998 at 11:25 am
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
PIC Value : 0.68+0.63
Comments : AGR43. CA microsatellite. Trimed to Rsa1 fragment. (2 polymorphic loci?).
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AccTgcAAgAAATTAggAAcTc at 3 length 22 bases, Tm 58
Reverse primer details :
cTAgTgTTTcAAATATgTcggA at 163 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 181 bases.



Length of sequence : 211  Consensus sequence is :

..

GTACCTGCAA GAAATTAGGA ACTCCTTTGT TGTTAAATCA CTACAACACT 
ATAAGGACTA TAAAAACCAT CTTTAACATA ATGCAACACA CACACACACA 
CACACACACA CACACACATC AAGGTTTGAG TGCTAAGTA1 ACTAGCATCC 
CCCCTAGGTA GTTCCGACAT ATTTGAAACA CTAGTTGGCG CACATAACTA 
CATTATAGTA C

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wmc18(1D6) Plate reference : 1D6
WMC locus number : WMC18 Length of sequence : 398
Last updated : Thu, Jun 11, 1998 at 11:27 am
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
PIC Value : 0.77
Comments : AGR44. (CA)(CT) compound microsatellite. Expected fragment size 230.
PCR Comments : 61C anneal, polymorphic on acrylamide;
Forward primer details :
cTggggcTTggATcAcgTcATT at 49 length 22 bases, Tm 69
Reverse primer details :
AgccATggAcATggTgTccTTc at 258 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 230 bases.



Length of sequence : 398  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAACATGT CGTGCTGCCA CATGGATTCT 
GGGGCTTGGA TCACGTCATT CCAGAGTGAG AAACAGAGAG AGAGATGTCC 
TGCAACAGAG AACAGAGGAA GCTTCATGAA CAGAACACC3 ACTCCAGATC 
GAATTGAATC GAACACACAC ACACACACGC ACGCACACAC ACACACTTAG 
AGAGAGAGAG AAAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAA 
GAGATGGGAA GGACACCATG TCCATGGCTG CGGCCCCAGG GCCAGAGCAA 
1TTGTCCATG GCCTCCTCAA TAAAAAGAAG 8TACTCATTC CAAGTGTAGT 
TAGCTGGTCA AAAACA8TTT TGGTTAGTCC ACGCGCCTCG AGGGATCC

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wmc(1E6) Plate reference : 1E6
WMC locus number : WMC Length of sequence : 210
Last updated : 26 Sep 1996 at 8:59
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR45. No obvious microsatellite.
PCR Comments :



Length of sequence : 210  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAAGGCTC AGCTGTGGCT AGCTGGTGGC 
GTGGGGCCCT GGCGGTTTCA GGGCAAGGCG GAGGTGGCTG CGGTGGCTCG 
GCGGATCGGG GCGGCGGCGT AGAGGAAGGC AGGGCGATGC GGGGCTGTGG 
CAACTACA4G AGGAAGAAGG TGGGAGAGGA GGTGGGTAGT CCACGCGCCT 
CGAGGGATCC

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wmc19(1F6) Plate reference : 1F6
WMC locus number : WMC19 Length of sequence : 509
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR46. CA microsatellite. Faint artefacts found after PCR at 61C and 51C anneal.
PCR Comments : 61C anneal, polymorphic product on agarose; 51C anneal, product;
Forward primer details :
cTgAcATgcggcATTcAcTTcc at 85 length 22 bases, Tm 69
Reverse primer details :
AggcTTAgAAcAcAccgAcAcg at 216 length 22 bases, Tm
Expected size of amplified fragment (Chinese Spring=) 153 bases.



Length of sequence : 509  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCGG ACTARTGGCG GACGCCGCTC GGTTTAAATA 
ACTGCTCCTT GACCTCAAGC GAGGAGCCGG AACGCTGACA TGCGGCATTC 
ACTTCCCCGA CGGATGAGGC GGCCATCGGA CTAAAACTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTTCCT 
TTTTTTAGAT TTTACCGTGT CGGTGTGTTC TAAGCCTCCA ACAACGCTTT 
CTTGTTAGTT GTCGGTGCAT ATTATGTATT GTTGGGTAGT CCA1G1GTTT 
T1GAT1AG1G T1GTTTCAGA G4GT1AGCGC CCACATTYTT GCCCGAGCAG 
AGCGGATGCG ACCG1T1ATT GA1GCCGGCA 1TGAAGCGGT G1GCGGCCGA 
GAGCGCCA1T TGTGCCG1TT CCCGATT1AA GCAACTGTCA 1GCATTCAAA 
TGACACAACG ACCGCCCGTT CTTCCGT1TA CCACAYAGTC CACGCGCCTC 
GAGGGATCC

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wmc20(1G6) Plate reference : 1G6
WMC locus number : WMC20 Length of sequence : 285
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR47. CA microsatellite. Trimmed to Rsa1 fragment.
PCR Comments : 51C anneal, product;
Forward primer details :
TTAAAAAcAcgcggATcTTcTc at 169 length 22 bases, Tm 62
Reverse primer details :
gTAcTcAcATATTTcTcggTcT at 264 length 22 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 117 bases.



Length of sequence : 285  Consensus sequence is :

..

GTACTGAGCG CGTATGCTAT CAACAGGTCT AATTGACACG AAGATTTGTG 
TATCACAAAA GAAGTAGAAG GAAACTCATT AGTCAGTTGC ATATATAGCA 
ATGATGAACC ATAAATATTC CATCCAAACA AAATTAATTT CACATCAAGA 
CTAGAAGCAT AATTATTTTT AAAAACACGC GGATCTTCTC TCTCACACAC 
ACACTCACTC ATCCCTATGA ACACACACAC ACACACACAC CTTACCCTAT 
GAGCACCTTC GAGAGACCGA GAAATATGTG AGTAC

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wmc(1H6) Plate reference : 1H6
WMC locus number : WMC Length of sequence : 353
Last updated : Thu, Aug 21, 1997 at 2:41 pm
Owner : Nickerson Date at Agrogene : 10.5.96 Public Domain : Yes
Comments : AGR48. No obvious microsatellite, beyond short GA stretches. Bases 21-181 (5' side of microsatellite - small) match part of 5H4 (complement).
PCR Comments :



Length of sequence : 353  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTGG ACTAGCACGT GGCCAAGAGG GGTCTGACAA 
ACAAGGGAGA GAGGTCGAGA GAGACGTACA GAGAAAATGC AGACATGGGG 
AGGAGAGAGT GTATGTTTGT CATAGAGAGA TCCCCTAGAG ATTGTGATGG 
GACTACATGG GTGGGAGATC GAGTGGCAAG GTGTGTGCGC GGTAGAAGAT 
GCCCCGAGAG ATATCGAGAT AGACGTATGG ATGGAAGGAG ACAATACGTG 
TGTTCCTGTT GGCTAGTAAG AGATAATCAT ACTTAGGAGG GGGAGTGCTT 
GCGCCTATGA TGGATTGAGG GATTATGGTT AGTCCACGCG CCTCGAGGGA 
TCC

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wmc(2A7) Plate reference : 2A7
WMC locus number : WMC Length of sequence : 141
Last updated : 26 Sep 1996 at 8:59
Owner : Wang, Utah Date at Agrogene : 14.8.96 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :



Length of sequence : 141  Consensus sequence is :

..

AACGGGGGAT CTACCGGCAG ACACGGGTCC AAAGCTACTC ATGCGCTTAG 
TAGCCAACAA GCGGTCAAAC CAACCAAGCC TCCGCCCATG CATAGCACGG 
GAGGATCACT TGCACGAAGG CGTCCTGCAA GGCCAAATCA C

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wmc(2b7) Plate reference : 2b7
WMC locus number : WMC Length of sequence : 234
Last updated : Tue, Aug 26, 1997 at 4:03 pm
Owner : Wang, Utah Date at Agrogene : 14.8.96 Public Domain : Yes
Comments : Microsatellite too close to end to design primers(CA)23 7 to 52. Duplicate of 3G7.
PCR Comments :



Length of sequence : 234  Consensus sequence is :

..

AGCAAACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CAAAGAGATC GCGCCAGCAA ATGCAAAGTC ATAGCAGACC GAAGCTATGC 
GTAGGCGGAT GAAGATTTTT TTTTACCAAA GTGACGATAT ATAATAGTGA 
CCATCCGCAA CAACATTTCT CGATAACATC AGGACTCCGA GAAAGGATCT 
CCAAAAGCGA CGCCTTCAGG AAGGAGTAGT CCAC

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