Wheat microsatellite WMC 25/06/01

wmc(4e9) Plate reference : 4e9
WMC locus number : WMC Length of sequence : 215
Last updated : Mon, Apr 7, 1997 at 10:24 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Duplicate 4d9. No substantial microsatellite.
PCR Comments :



Length of sequence : 215  Consensus sequence is :

..

NNGAGGGAGT CGCCGACTCC AGATCCAGCG CCGCCAGGAT TCAGCCCCAC 
CGCCGCCGCC GCCGGGGATT CGCTTCAGCG CCGCCGGGAA TTCGCATCGC 
CGCCGCTGGT AGTTGCCTCC GCGCCGCTGC TGGGAGTCAC CCAAACTTGC 
GTCGGCAAGA GGTAATTTCC TCGGCCAGCT CATGCAGTGC TTTGCTAATT 
CTTCTCTTAA AGTAC

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wmc193(4f9) Plate reference : 4f9
WMC locus number : WMC193 Length of sequence : 782
Last updated : Lun 2 Nov 1998 at 16:58
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Multiple inserts (2) in this clone joined 330-347 by linkers. (CA)30 382 to 441. Primers are for second insert. Bases 217-327 (ie 3' die poly C microsatelllite) match part of 3D9. Bases 264 to 300 show homology to 17a10 bases 90 to 126 (complement) (37 in longest run, maxScore = 45). Bases 224 to 300 show homology to 22d8 bases 40 to 116 (complement) (77 in longest run, maxScore = 68), true duplicate, 4f9 has priority.
PCR Comments : 51C anneal, product;
Forward primer details :
cgTggAcTAAcAAgTcATTT at 348 length 20 bases, Tm 55
Reverse primer details :
gTcATcATTTTccccggcAT at 444 length 20 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 116 bases.



Length of sequence : 782  Consensus sequence is :

..

GTACAATCTA TACTTGTGGG AGAAAAGGAC CAACGAGGGA ATGCCTTATT 
TTCAAATGCG AGAGGGGTGT GGGTATCTTT TTGCAAAATC GCCATAGTTT 
CCTTCCTATC CATCGGATAT AAATCGGATG GCTATATTGC AGGATGGCAG 
GAACACCATC ATCACCAACT CTGTTTTTTA TAAGTAGAGA TATGTGAGTG 
TCACTGCCCC CCCCCACACA CCCACACTTC CCAACACCGA AGGAGTAGGG 
TGACACCTCG ATCTTGATAC TAATCTATCG ACTGGCTTCT CTCTCAAACA 
TACACACACA CACACTAACA CTACCCGGTT AGTCCACGCG TGGACTACGT 
GGACTAACAA GTCATTTATT GATAATGTAT GCACACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC AATATGCCGG 
GGAAAATGAT GACTACATAC TAACATGTGT CTCTGTCTGT GGTATTTATT 
TCAGTTACCG TTGGAAGCTG GGGCTTGCAA ACTGCTGGGG GGCTCATAAT 
TCATTCTTGT TGGATGGTCG ATGGTCCATT TCCAGCCAAA CGCTTAACAC 
TAAAAGGGAC CATTGTTTGA ACCTAAACTT CTGTTGACCA TACCCGTTGA 
ACGCTTTTGG CCACAATGGG TTCCCAGAAA ACCGAAATTT CCTCAAAGGA 
AGAACCTTCC CCTCGAACAA CCTAAAAAGG CTCCCTCCTT TTTAATTCTC 
CCTCTTGAAC TTAATGGTGG AAGGGCCGGT AC

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wmc(4g9) Plate reference : 4g9
WMC locus number : WMC Length of sequence : 431
Last updated : Tue, May 20, 1997 at 10:38 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' and to insert at 3'. Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 5H10 9H3, 4b7, 4g9. (GA)8 188 to 203, (GT)8 309 to 324, (GT)9 327 to 344.
PCR Comments :



Length of sequence : 431  Consensus sequence is :

..

GTACAGATGA TAAGTTTGCT GACTACTAAA GTAAAGTGGA ATTTGTCCAT 
GTTATGCAAG TAAATAAAGG TGATTGTTTA TCATATGGGT AAGGTTGGAT 
GGAGGGTAGT AGGAACAAAT GCTCCACCTA GAGCATGTGT GTGTGAGATG 
GAGTCTTAGT GTGTGTGTGG GGTCTGTGTG TGTGTGTGAG AGAGAGAGAG 
AGAGGAGAGA CAGAGAGAGA TGGAGTCTTA GTTTTTGTGT GTGTGTGAGA 
GAGAGAGAGA TGGAGTTCTT AGTGTGTGTG TGTGGGGGGG GGGGGTGTGC 
GTGCGTGCGT GTGTGTGTGT GTGTTTGTGT GTGTGTGTGT GTGTGAGAGA 
GATGTAGTCT TAGTGTG

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wmc(4h9) Plate reference : 4h9
WMC locus number : WMC Length of sequence : 269
Last updated : Mon, Apr 7, 1997 at 10:27 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Timmed to Rsa1 at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 269  Consensus sequence is :

..

GTACTAGCTA TGACCAAAAT TCTAAAAGGT CAAGTTTGTT CGTTCTTGAC 
GGCCAACTGT TGACCTTCTG AATTTGGTCA AAAAAGGTCA ATAAACGATA 
AATATGACGA ATCAACGACC AAAATGGGGA GTCATAATTG AGTGTATTTC 
TTGTAGTGGC GGCGGCTGCG AGGGGAAGGG GCGCGGCGGC GGCGACGCAA 
AGGAAGCGGC GGTGGCGGAT GGTGCGGAAG GTGGGAATAG AATCGGCGTG 
CTAAACTGAT ACCATGTAC

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wmc(4a10) Plate reference : 4a10
WMC locus number : WMC Length of sequence : 169
Last updated : Mar 30 mai 2000 at 9:54
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 69-161(ie 3' side of microsatellite) match part of 8h7, bases 56-155(ie 3' side of microsatellite) match part of 3d2. (GA)10 143 to 162. No room for primers Bases 98 to 131 show homology to 17a11 bases 1 to 34 (34 in longest run, maxScore = 32).
PCR Comments :



Length of sequence : 169  Consensus sequence is :

..

CGACGCGCGT GCGACATAAC TGAATATTGT CAGAGAGAGA GAGAGATAGA 
GATAGGATAG AGAGAGAGAG AGATGGAGGG AGAGGGAGAG GGACTAACAA 
GGTTTGTGTG TTGGATGCGT GCGACAGAAC TGAAGATTGT CAGAGAGAGA 
GAGAGAGAGA GATGGAGGN

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wmc194(4b10) Plate reference : 4b10
WMC locus number : WMC194 Length of sequence : 166
Last updated : Ven 12 FŽv 1999 at 15:25
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. 3' of microsatellite is similar to 9e1. Bases 1-99 (ie. 5' side of microsatellite) match 9e1. Bases 72-166 match 4D10 (CA)8 73 to 88. Duplicate 14b6. Bases 135 to 155 show homology to 14b6 bases 135 to 155 (21 in longest run, maxScore = 67). Bases 63 to 89 show homology to 26f2 bases 113 to 139 (complement) (27 in longest run, maxScore = 76). Near duplicate with 26f2, 4b10 has priority.
PCR Comments : 51C anneal, product;
Forward primer details :
ATAggAgTAggTcgATgTgTcA at 23 length 22 bases, Tm 58
Reverse primer details :
gTATcTcAcTTccTgTggcTTT at 134 length 22 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 133 bases.



Length of sequence : 166  Consensus sequence is :

..

GTACACTACA CACCATATCT CTATAGGAGT AGGTCGATGT GTCACTCTCT 
CTAACACACA CCTGCTGTTA AACACACACA CACACACACG CACACACACA 
CAGGTTCATA GAATACGAAA ATCGTGCGAG TGCAAAGCCA CAGGAAGTGA 
GATACGAATG GAGTAC

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wmc195(4c10) Plate reference : 4c10
WMC locus number : WMC195 Length of sequence : 448
Last updated : Tue, May 20, 1997 at 4:53 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at both ends (removing mostly Ns). Small microsatellite. (CCG)5 82-96, (CCG)4 108-119.
PCR Comments : tested 61C anneal, no product; 51C anneal, product;
Forward primer details :
AgAgTTcAgATcTAcAAggcgg at 16 length 22 bases, Tm 62
Reverse primer details :
gggccccAAATTAATTAgcAcc at 212 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 218 bases.



Length of sequence : 448  Consensus sequence is :

..

GACCAAGCAA AACAGAGAGT TCAGATCTAC AAGGCGGAGG GGATTGGGAC 
TAGAGTATCG AAATTCACTC ACCACCATGG CCCGCCGCCG CCGCCGAAAG 
GGGGCTTCCG CCGCCGCCGA GAAAGAGGGC TCCCTCCGCT GTAGCCGCCC 
CAGGAGATGA AGATGTTCGG GCAGTTTCGG ACAGTTCTAG TCGGGCAGTT 
TCACAAGATG CGGTGCTAAT TAATTTGGGG CCCAAATTAG GTAAGACATG 
ACTTAACGCA AATTGGACTA GTTGACTAAG GTTCTATCGC ATGTGTTGGC 
GGTGAGATAT TTTCTCTCTG CAAACTGCTC TCACGGAAGA TATCTTTCAC 
CTAAAGATAT CTCACTGGCT CTTCCCTTCC CAGGGAAATA CATCTCACCA 
TGCTATTGGC CCTCGGGCCC CACTGGAGCT ATATGGGATT ATTCCCAG

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wmc196(4d10) Plate reference : 4d10
WMC locus number : WMC196 Length of sequence : 182
Last updated : Jeu 27 Aož 1998 at 12:05
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (CA)14 75 to 102. Bases 86-182 (ie. 3' side of microsatellite) match 4b10. Bases 145 to 182 show homology to 19c3 bases 27 to 64 (complement) (38 in longest run, maxScore = 53). Bases 111 to 130 show homology to 10b9 bases 55 to 74 (complement) (20 in longest run, maxScore = 36).
PCR Comments : 61C anneal, product;
Forward primer details :
ccgTAcggAgTcAggTcgATgT at 21 length 22 bases, Tm 67
Reverse primer details :
gcTTcgcAcTTgcAcgATTTTc at 133 length 22 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 134 bases.



Length of sequence : 182  Consensus sequence is :

..

GTACACTACA CACCATCTCT CCGTACGGAG TCAGGTCGAT GTATCGCTCT 
CTCTAACACA CACATGCTGT TAAACACACA CACACACACA CACACACACA 
CACGCACACG CACACACAGG TTCATAGAAT AGGAAAATCG TGCAAGTGCG 
AAGCCACAGG AAGTGAGATA CAAATGGAGT AC

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wmc(4e10) Plate reference : 4e10
WMC locus number : WMC Length of sequence : 706
Last updated : Mon, Apr 7, 1997 at 10:31 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 706  Consensus sequence is :

..

CTTTGCCTTG GACGGTGTGG TTTAACCCAA AAGGCCCAAA ACAATTGGGG 
AGATGGAATT GGAGATGAGT GGGTTACCAT GTATTCTTGC CCAGAATCAA 
ATTTATTGCA CCTTGGATCC GTTAATTGGG GTCTGTAGGT GGTGTTAGAC 
AGTGCGGACG ACCGCCACCA TGTGAGGGTT GCACCCAGTG TGCTTCAAAG 
GGGGCGCGTG GTGAACAGAG GCCGACGGCG GCGGCTGCGG CAAAGAGTAT 
TAGAGGGCGT CGACGTCGAG GGCGCACACG AGTGGAGCAG GACGATGGAC 
TGGCGACCTT GGCATCGGCC ATGGGGTCGA TCCGGCCAAC TCGATCCAAA 
GCAGAGAAGC ACGATACGGG TGTAGGAGGT TGGAGATTGA GGAGGGTGCG 
GGTTGGGAGG GGAAGGAGGA CAGGAGGTCG GCGGATGGCC GGAGAAGAAG 
ATCAGCGGCG GCGGCGGTCG CGGCTGGAGA GGAAGCGGCC AGAGAGGATG 
TTGTGCGTTT ATTATAGCCA GCTAATGAAA GGATCGTGGG TTGAATACTA 
AAGAGTGCAG GATTACTTTA GTAAGAGTGA AACGTTTTCT CATATATCCA 
CTTAAAAAGG GAGTGCGGGT TGAATAGCAA AAATAGAAGG GACGTTTTTG 
TAAAAACGTT GCGACGGTGA ACCCGGAGAG ATTTGCTTGT GGTTATGATG 
CTGTAC

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wmc(4f10) Plate reference : 4f10
WMC locus number : WMC Length of sequence :
Last updated : Wed, Mar 5, 1997 at 8:49 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : No sequence 5.3.97
PCR Comments :
No sequence available for this clone
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wmc(4g10) Plate reference : 4g10
WMC locus number : WMC Length of sequence : 295
Last updated : Wed, Aug 20, 1997 at 1:01 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends (1 N in linker at 3' end). Core is similar to 6a8(1). (GA)8 146 to 161, (GA)10 194 to 213, (GT)11 172 to 193. No room for primers.
PCR Comments :



Length of sequence : 295  Consensus sequence is :

..

GTACCCTTTT GTTGAAGGTC AAATCGAGCT TTTCCTGGGA AGTATGGCAC 
ACCATCATTG CCAACTCGTT TTTTTATAAG GGTAAAGATG ATAAGTTTGC 
TTACTACTAA AGTAAACATA TGTGTGTGTT TGTGTGTGTG TGTGTGAGAG 
AGAGAGAGAG ATGAAGTTTT TGTGTGTGTG TGTGTGTGTG TGTGAGAGAG 
AGAGAGAGAG AGATGGAGTC TTAGTGTGTG TGTGTATGTG TGTTTTGTGT 
GTGTGGGGGG TGGGTGGGTG TGCGTGTGTG TGTGTGAGTT TGTAC

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wmc(4h10) Plate reference : 4h10
WMC locus number : WMC Length of sequence : 310
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends (Ns in linker at 3'). Multiple inserts (2) in this clone, joined 103-120 by linkers. First insert matches 1a3 and 5e1, 5B4, 5d4. No substantial microsatellite. Bases 120 to 142 show homology to 24b8 bases 167 to 189 (23 in longest run, maxScore = 19).
PCR Comments :



Length of sequence : 310  Consensus sequence is :

..

GTACACCGAC CGATCTCTCG CTATACATAT AGCTAGCTAG GTTCTTATAA 
CTCGTTTTTA CCCACACGTC AATCGATCTA CCTCATTAGT TGTGTCTCTC 
CCTAGTCCAC GCGTGGACTA CCGCTCTCTC CCCGCTCTTT TTCGCCTCGC 
CGCCGCCGCG CCACCATGCC GCCGCATCGC CGGGAAACTT GGGGATACCG 
CGGCGTCCGC GCGCGCCCCT CTAGCGGCTT CTTCGCCGAG ATCTGGTTCC 
ACGGGGATGC ACCTCTGCCT CGGCAATTTC GACACCGCCA ATGAGGCCGC 
CCGCATGTAC

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wmc(4a11) Plate reference : 4a11
WMC locus number : WMC Length of sequence : 363
Last updated : Mon, Apr 7, 1997 at 10:34 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Matches 4b11 No substantial microsatellite.
PCR Comments :



Length of sequence : 363  Consensus sequence is :

..

NNTCCTCGTC AATCCCTTCG CACAGCCGCC GCCGCGGACC AGACCTTCGA 
CCGTGGCGAC GACGGCGTCC CTCCTGTCGG TCGTGCCGCC GTCTAAGCCC 
AATATCGTCT CCATCCACAC AGCGGCAGCC GCATATACTT TTTGTCGCCC 
CTCCCCTAAT CCAGTTTCCG TCCAAAGTCG CCGCCGCTAA AGCAAGGGAT 
CCCACCGGAC ATCGCCGCCG CCGCCGCAAT CAAGTGATCC CGCCGGACTC 
GCCGCCGCCG CCATCAAGGG AGCCCGCCGA ACCTCGCCGC CGCCGCAATC 
AAGGGATCCC GCTGGATTGC CGCCAGGTTT CTGGCCTGCC GCCAGGAGCC 
GCAGGAACTG TAC

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wmc(4b11) Plate reference : 4b11
WMC locus number : WMC Length of sequence : 363
Last updated : Mon, Apr 7, 1997 at 10:35 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Matches 4a11. No substantial microsatellite.
PCR Comments :



Length of sequence : 363  Consensus sequence is :

..

NNTCCTCGTC AATCCCTTCG CACAGCCGCC GCCGCGGACC AGACCTTCGA 
CCGTGGCGAC GACGGCGTCC CTCCTGTCGG TCGTGCCGCC GTCTAAGCCC 
AATATCGTCT CCATCCACAC AGCGGCAGCC GCATATACTT TTTGTCGCCC 
CTCCCCTAAT CCAGTTTCCG TCCAAAGTCG CCGCCGCTAA AGCAAGGGAT 
CCCACCGGAC ATCGCCGCCG CCGCCGCAAT CAAGTGATCC CGCCGGACTC 
GCCGCCGCCG CCATCAAGGG AGCCCGCCGA ACCTCGCCGC CGCCGCAATC 
AAGGGATCCC GCTGGATTGC CGCCAGGTTT CTGGCCTGCC GCCAGGAGCC 
GCAGGAACTG TAC

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wmc197(4c11) Plate reference : 4c11
WMC locus number : WMC197 Length of sequence : 380
Last updated : Wed, Aug 20, 1997 at 1:04 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases131-380 (ie. 3' side of microsatellite) matches 6d5 , highly related to 2e10, 1g12. . (GA)9 149 to 166, (GT)8 133 to 148.
PCR Comments : 51C anneal, product;
Forward primer details :
ccTAATTAAAAgcTgTcAcgAg at 63 length 22 bases, Tm 58
Reverse primer details :
gcAATgcATATggcTgAAAgAT at 206 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 165 bases.



Length of sequence : 380  Consensus sequence is :

..

NCTTTCCAGG ATGAATGGAA AGGAGATTAA TAACTTAAGA AAAAAGTTAG 
ATTACAGTGG AACCTAATTA AAAGCTGTCA CGAGAAAATA AATAATTCAA 
CGGGTCCCAC TTGTGTGTGT GCGCGTGTGT GCGTGTGTGT GTGTGTGTGA 
GAGAGAGAGA GAGAGATAGA GAGAGAGAGA CCCATCGGTC GATGCTCATA 
AATACATCTT TCAGCCATAT GCATTGCTGA TGCTCAGTGA AACTTATATA 
GTTGACACAA TCATATAGAA CATCATAGCA CACTGAACTT GCATACAAAA 
ATTTCACGCA GGCGACACAA TCAGGATGGA AGCAGTGGAT TGCTACGGGA 
AGGGCTATGT TGAAACCAAC GAAGTCGTAC

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wmc198(4d11) Plate reference : 4d11
WMC locus number : WMC198 Length of sequence : 204
Last updated : Tue, May 20, 1997 at 5:32 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)27 67 to 120.
PCR Comments : 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
cAcgcTgccATcAcTTTTAc at 27 length 20 bases, Tm 61
Reverse primer details :
TTgAAgTggTcATTgTTgcT at 166 length 20 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 159 bases.



Length of sequence : 204  Consensus sequence is :

..

GTACTCATGG TTTTTGTTAA GAAAAACACG CTGCCATCAC TTTTACCCCA 
TGTGTTTTCT CGGTTTCACA CACACACACA CACACACACA CACACACACA 
CACACACACA CACACACACA TTTCAGCTTC GACTTCGAGC TTTCAAGTAT 
TCAAATATTC CAACTAGCAA CAATGACCAC TTCAAGGAAA GAAGAAGATG 
TCNN

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wmc(4e11) Plate reference : 4e11
WMC locus number : WMC Length of sequence : 309
Last updated : Mon, Apr 7, 1997 at 10:37 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 309  Consensus sequence is :

..

NNGCGCGGCG GCAAGGGTAG GAGGCAGCGG CAACACTAGA GGGAAAGCGC 
GGCGGCGGCG AAAGGTCAAG GCGGCTAGGC TACGCTATGC GGCGAGGAAA 
GAACGCAGCA GCGGTGGGAG GGAGCGCGGT GGCGGACTCA ACCGTTGGTG 
GCTCATCAAG TGAACCGCAA AATGGCAGTT TGCAATGGTA AGCAAAATTA 
TCCATTGGGT TAGTATAAGA TTATCATAAA GCATAAACAA CAGCCTAGAC 
TCACGTGGAA AATATTTAAA TCCTTTGACA AACGATTCAG TGAGAAGTTG 
GTGTTGTAC

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wmc(4f11) Plate reference : 4f11
WMC locus number : WMC Length of sequence :
Last updated : Wed, Mar 5, 1997 at 8:49 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : No sequence 5.3.97
PCR Comments :
No sequence available for this clone
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wmc(4g11) Plate reference : 4g11
WMC locus number : WMC Length of sequence : 347
Last updated : Mon, Apr 7, 1997 at 10:38 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end (removing mostly Ns). No substantial microsatellite.
PCR Comments :



Length of sequence : 347  Consensus sequence is :

..

GTACAGCGCT AATGGCCGCT AGTAATTGCA CCATTAGCCC ATATAAAAAT 
TTCTTTAATG AACCAACTAC GCCGAACATA GTAGGCCAAA GCCCAATAGC 
CCATACATGT AAGCGTATAG TTGTGGCTGA CTATTGGTTT GCCTCTGCAC 
AACGTGGCAC AATGTGATAG ATCCGATCGA TCATTGATCT ACACGTGTAT 
TCTCACGAAC TGCTTTAGGG CGTTTGCCGC CGCCGCCGCT CATGCCATCC 
GCCGTGGTGC GTGCCCTTCT AGGGCGTTTG CCATTGCCGT GGTGGCGGCT 
CGCCACGACG ACGGTGCCAT CCCACTGGTC TACAGTCCGC TATCCCC

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wmc(4h11) Plate reference : 4h11
WMC locus number : WMC Length of sequence : 339
Last updated : Mon, Apr 7, 1997 at 10:38 am
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 339  Consensus sequence is :

..

GTACGCGGAT AACAGCTACG TCATCTGTCC TCGGGGAAAG ATGGCTATCC 
TCGCCCAGAT CCGTCTGCTG CATGGCGGTG GGTCACAACA CATCTTAGTT 
TGTAGGAAGA TTCCCCGCTC TGAAATTGTT TCTATCTGGA GCTTCTTCCA 
AACAGGTTGG GATTCTAGTT TCAACTGGAT GTGTCCCGGT AAGATCGTTA 
ACCGACTGCC GGTCGGCGGT GGCGGAACTT GTCCGGTGGC CAGCGGCGAA 
GTTCTGTCTG GATCTCGGGG CGGCGGTCCT TTTATGGTGG CGGCTGGTGA 
AGCTATGGCT TCCCGCGGCG GCATGGTGTG GTGTAGTAC

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wmc199(4a12) Plate reference : 4a12
WMC locus number : WMC199 Length of sequence : 667
Last updated : Tue, May 20, 1997 at 5:47 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end (removing mostly Ns), trimmed to Rsa1 at 3' end. Compound microsatellite (NA)10(CA)3(GA)(CA)2(TA)(CA)6.
PCR Comments : 51C anneal, product;
Forward primer details :
cAcAcTcATAgTTATTAcgccT at 297 length 22 bases, Tm 55
Reverse primer details :
cTATTTAcTcAcTgAcTgggAA at 378 length 22 bases, Tm 55
Expected size of amplified fragment (Chinese Spring=) 103 bases.



Length of sequence : 667  Consensus sequence is :

..

CTTGTTTTAT CGGGCGGTTC GAAGGCGAGG TTGAAAACTT GTGACAACAT 
CTGGTTCCGG GAGACCGTCA CAGTTGTTTA TGGAGAGGAT ACGGGTGCGG 
ACAAGCCCCT TCATTGCGGG TGAGCGATGT TGGGGGGGTC ATGGTGGCGT 
GCATTTTGGG TCATCAGACC CGGATGGTGA CTGAGAGATC ACCATATGCG 
GTGGTGAAAT TACCGCAAAG ATGCGTTTGT AGCCAGTACC GAATCAGGCA 
CCATTCGCCA TTCAGATTCC TCGAGGCGTG GGGAGGGACA TCGGTGCACA 
CTCATAGTTA TTACGCCTGA TAGAGACAGA GAGATATACA CACAGACACA 
TCACACACAC ACACGACCGA GACATTATTC CCAGTCAGTG AGTAAATAGA 
ATGGACGGTC CGTTGGAACT ACNTTCGGGG GCGACGTGGA AAGGATAGGA 
CCCTCCGGCA TACACCGGAG GCAGACGAGA TCTCCTTGAT ACATAGTTAG 
GAGAGCAGGA ACCGAATACG AGCCGCCGCG GCCGATGGAT CTGGGCCGCT 
GGAGGTGGCG CACATCTTTT ACTGTACACC TCAGCCGGTG GGGAACCCGA 
GACGGCGACC GAACCAGATG GCAGCTCGGT CGGGGTTGGT AGAGATAGAG 
GATATGGGAG GTGGTAC

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wmc(4b12) Plate reference : 4b12
WMC locus number : WMC Length of sequence : 131
Last updated : Wed, May 21, 1997 at 4:12 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end, and to insert at 3' end (removing mostly Ns). Similar to 1 side of 9d1. (GT)17 76 to 109. No room for primers.
PCR Comments :



Length of sequence : 131  Consensus sequence is :

..

GTACCTCGTA TACTTACTAC TCCCTCCGTC TAGGTGTAAT AAGTCACGTT 
AGAAGGTGCA ACAGGCACAA GGTGCGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTC AGAGAGAGCG ACAGATCGAC C

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wmc(4c12) Plate reference : 4c12
WMC locus number : WMC Length of sequence : 355
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 5H10 9H3, 4b7, 4g9, 4c12. (CA)8 59 to 74. Bases 73 to 121 show homology to 21d9 bases 87 to 135 (complement) (49 in longest run, maxScore = 45). Bases 11 to 48 show homology to 21c12 bases 220 to 257 (complement) (38 in longest run, maxScore = 65).
PCR Comments :



Length of sequence : 355  Consensus sequence is :

..

NCCACACACG CACACTAAGA CTCCATCTCT CTCTCTCACA CACACACATA 
CACACTAACA CACACACACA CACACGCACG CACCCCCCCA CACACACTAA 
GACTCCATCT CTCTCTCTCT CACACACACA CACAAACTAA GACTCCATCT 
CTCTCTCCTC TCTCTCTCTC AAACACACAC ACACCCTACA CACACACTAA 
GACTCCATCT CACAAACACA TGCTCTAGGC GGAGCATTTG TTCCTACCAC 
CCTTCATCCA ACCTTACCCG TATGATAAAC AATCACCTTT ATTTACTTGC 
ATAACATGGA AAAATTCCAC TCTACTTTAG TAGTCAGCAA ACTTATCATC 
CGTAC

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wmc200(4d12) Plate reference : 4d12
WMC locus number : WMC200 Length of sequence : 274
Last updated : Wed, May 21, 1997 at 4:26 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at the 5' end, and to insert at 3' end. (GT)8 56 to 71, (GT)10 97 to 116.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
TAcgTgAgAgAgTcccTAAc at 2 length 20 bases, Tm 54
Reverse primer details :
cTccTcAcgAggcTTccATA at 202 length 20 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 220 bases.



Length of sequence : 274  Consensus sequence is :

..

GTACGTGAGA GAGTCCCTAA CTAGAGAGAG AGGGAGAGAG GGAGAGAGAG 
AGAGAGTGTG TGTGTGTGTG TGAAATGGAA AGAGTGACTA CCCACAGTGT 
GTGTGTGTGT GTGTGTGACA CAGACATGGA CAACACACGA CTAAAGTAGA 
CGAGAAAGCG GGTGTGTCCT CTGTCTAACA TATCACACAT GCACCTTCGA 
CTATGGAAGC CTCGTGAGGA GACCGTGTGT GGCCGTCTGC CACCCAGAGA 
GAAACACCCC TACCCGTTGG CCNN

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wmc(4e12) Plate reference : 4e12
WMC locus number : WMC Length of sequence : 261
Last updated : Wed, May 21, 1997 at 5:22 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimed to Rsa1 at both ends. (CT)12 209 to 232. No room for primers.
PCR Comments :



Length of sequence : 261  Consensus sequence is :

..

GTACTACGGC TGGAGCCGCA ACTTGACCAC TCCCGTCGCG AACGACGGCG 
TCCACCCGGG TGGTGGCACC GGGTCGTACA TCTATGCAGC CGCGTATCAA 
ATGTGCTGGT GATGTTCTGA TAGGAAGAGT ATCGGCTGCG GCTATGGTAA 
GCTATCATCC CCAATGCAAA TGGCTGGTAT ATGGTTTTCC GAGCGGCAAC 
CGATCGGTCT CTCTCTCTCT CTCTCTCTCT CTCACACACA CACTCCCTCC 
ATACACGGTA C

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wmc201(4f12) Plate reference : 4f12
WMC locus number : WMC201 Length of sequence : 372
Last updated : Lun 5 juil 1999 at 17:23
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
PIC Value : 0.84
Comments : Trimmed to insert at both ends (remving mostly Ns). (CA)25 201 to 250. Bases 162 to 249 show homology to 26f10 bases 402 to 489 (88 in longest run, maxScore = 114).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cATgcTcTTTcAcTTgggTTcg at 85 length 22 bases, Tm 66
Reverse primer details :
gcgcTTgcAggAATTcAAcAcT at 312 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 249 bases.



Length of sequence : 372  Consensus sequence is :

..

TCTCGAGGCG CCAGGGCCCC TTAGGCAAAT GGACTACCAC GGACTTTAGG 
AGCTCAAATT TCAGAGGCTC ACAATCTCGC TAGACATGCT CTTTCACTTG 
GGTTCGGTTG CCATGTTTGG TTAGGCCAAC CTGAAGATTT GAATTTTATC 
TCTGTAAATG TTGTGACAGT TTGAATAAAG CATCGTGAGA ATGTCTAAAA 
CACACACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CCACACACAC ACACCCACAC ATCGCAGTGG CTGGAGCGGC CATGTTTGAC 
CGCGGCGGCG CAGTGTTGAA TTCCTGCAAG CGCAAGTGCC TTCCCTTTTT 
TTTTAGTCTC ACGCGCCTCG AC

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wmc202(4g12) Plate reference : 4g12
WMC locus number : WMC202 Length of sequence : 462
Last updated : Wed, Aug 20, 1997 at 1:09 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (CA)18 207 to 242, (CA)9 251 to 268. Bases 339-462 (ie. 3' side of microsatellite) match part of 1e2.
PCR Comments : 61C anneal, product;
Forward primer details :
TTgTgcAcATcAATgTAAgcgg at 8 length 22 bases, Tm 66
Reverse primer details :
TgcTTTTgTTTgTcgTcgAggT at 414 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 428 bases.



Length of sequence : 462  Consensus sequence is :

..

GTACATGTTG TGCACATCAA TGTAAGCGGA ATGGGTCCGT CATTCACCCA 
GGAAGTCCAA GGCTGAGGGG AAAAGGTCTA CTGCTTTAGC TTCTAAAGGA 
GCAGCATCAC GTGACAGTTT GGCAGGTAAT TAAGAGGAAG CCTCTGATCG 
AGAATCGTTC AAAATAAGTA GATTTTTTCT TGCGGGTAAA AAAGTATTAC 
TTGTTTCACA CACACACACA CACACACACA CACACACACA CAGAGAGAGA 
CACACACACA CACACACATG TATGTATTAT TTTGGGATGG CTTAAATGTT 
GTGTCAGTTT TGATTCTGCT ATCTAGATAG CACAACCTCA ACCAGTAAAT 
GGTAGAAGTT AAACTGCAAT TGCACAAATT ATATGAAATA CTGTAAGACA 
TGGGATGCAT CTCACCTCGA CGACAAACAA AAGCATCGGT CGTTATAACC 
CTGCGTAAGT AC

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wmc203(4h12) Plate reference : 4h12
WMC locus number : WMC203 Length of sequence : 400
Last updated : Wed, May 21, 1997 at 5:57 pm
Owner : Morell, Canberra Date at Agrogene : 5.3.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end (removing mostly Ns). (GT)10 67 to 86, (GT)34 289 to 356.
PCR Comments : tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
cTAcTTgcATgTcgAATAcccg at 96 length 22 bases, Tm 63
Reverse primer details :
ccATTcgTggATTTgTTTcAgg at 358 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 284 bases.



Length of sequence : 400  Consensus sequence is :

..

GTACATGTTT ATTGAGGAAC CCCAATTGCA AGTTGCCCAT GGTCTCGCAA 
TTAGGGGGGG TGCTGCGTGT GTGTGTGTGT GTGTGTTGAG GGTCCCTACT 
TGCATGTCGA ATACCCGTCT CTTGCGCTAG AAGTGTGTGT GCGCGCGTGT 
GTGTGTGTCT TTTTCTCAAC GGGTCCCCAG CTGCATGTCA AGCATCGACT 
CGCAAACTAG GAGGGTCTAT GTTTGTTGAG GACCCCCAGT TGCAAGTTGA 
CCATCAACTC GCAACTTCGG GGGATGTGTG TGTACTGCGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTCCCT GAAACAAATC CACGAATGGA ATGTACTCCC ACGCGCCCCG

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wmc204(3A1) Plate reference : 3A1
WMC locus number : WMC204 Length of sequence : 899
Last updated : Mar 17 Nov 1998 at 9:58
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to sequence. (GA)9 811 to 828, (GA)21 831 to 872. Matches 5a2, part match to 4c1. NB this sequence contains numerous ambiguities and a few differences to 5A2, if PCR fails, try designing primers to 5A2. Bases 668 to 732 show homology to 10h10 bases 127 to 191 (complement) (65 in longest run, maxScore = 124). Bases 668 to 740 show homology to 24h2 bases 67 to 139 (73 in longest run, maxScore = 126), true duplicate, 3a1 has priority.
PCR Comments : 51C anneal, product.
Forward primer details :
cATAcATcAAgTgTTcTcAAATcc at 641 length 24 bases, Tm 59
Reverse primer details :
ccAgTAgcTATgTgTTTgATcTcT at 876 length 24 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 258 bases.



Length of sequence : 899  Consensus sequence is :

..

XAGGAGGCAA TGGCAGTCCG CATTGCACTG CGTCGCTCCC GGGAGGACAG 
CGCCCAGCCG ACGGACGGAT TTGTCCGCCG CGACTCCATA GTGTCGGCTT 
ACCGGGCGCT CGGGTCCTCT GGGGTTGGAT CCTTGCGGTC CCGGTAGCCG 
GAACACCTCT CCTTCCCCAT CACTAGCCGG GCAGACTATA AGTGCCGATG 
GGGCCCGTAT CATGGTAXAA ATGGCGGCGG CGGAAGCGGC TAATGCGGGG 
CGCGGGCGGC CGCAXAXGAX GAAGCCATCC GTGTCCGCAT TGTGAAXAAA 
CGGCGGCGGA GGAACACGCA TGCXCTCACC CGAGAGCAGA ATCAGGAGGT 
CCGTGCCATG GCCGMACTGC CGacTCSWWS GAGGAGAgAG ACGgACCATG 
WTGGcGARGA CaGYTCCGAC GGSGAGCAgA TCCGGYTCGA TCCGTcTGSG 
CATTTGACCG GTTAxTCCAC GScTtGGACT AAMTTCAAAC MATGCCCCcA 
AGTTTcTAAC GRAGAgTCAA gACgAAAAAY KTKTgCCaaC CCCtATGCaT 
AAKTTCaTTG AACCCgCAag TTGATCaCxA gAACaTACaT CAAKTWKMYM 
AMKWSMWTWT CCCATTGTCA CCACAGATAA GCACGGCAAG ACATACATCA 
AGTGTTCTCA AATCCTGAAA GACTCAATCC GATAAGATAA CTTCAAAGGG 
AAAACTCAAT CCATTACAAG AGAGTAGAGG GGGAGAAACA TCATAAGATC 
CAACTATAAT AGCAAAGCTC GCGATACATC AAGATCGTAT CACCTCAGGA 
ACACGAGAAA GAGAGAGAGA GAGAGAGAGG GAGAGAGAGA GAGAGAGAGA 
GAGAGAGAGA GAGAGAGAGA GAAAGAGAGA TCAAACACAT AGCTACTGG

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wmc(3b1) Plate reference : 3b1
WMC locus number : WMC Length of sequence : 1117
Last updated : Fri, Jul 4, 1997 at 4:35 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. big insert. No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 1117  Consensus sequence is :

..

ATCCTTGTGC AGATCATCCA CTTCGCCGCT AAGTTCTGCG GTCTCTTCAT 
GCTGCTGCAG AAATTCTCCT TTCATAGCCA AGAGCTCAGC TACCTCAGAT 
TCGTCGAGGC TAGCCTTCCC ACGCCCCATA TTGGTGAGGT CGTTCAGAGG 
GGAATTTACC TCCAGGACGA GTCGGGAACC AGATCTGAAT GCTTACCCTG 
CCGCCGTCGA ACGAGGTCGC CACCGGAACA GCACCGCACG CCGCACGACC 
TGGAATTTTA CGGAGTGAGC GGTGTGCTCA CACAACCAGT CGGGCTCGAC 
GCGCTGCCGT CGAATGAGTC TACCGCCGCC TGCGCCGCCT ACTCCGCCGC 
CAACNCCACC AACACCGCTG CTGCCGACGA GAAAGGGAGG GAAGGGTGNG 
AAATCNCCGT CGCAGCTCCG ATCTGTCGCA CCGCCGAAGA AAACTANTCA 
CGCNTGGANT AACCAAACCC NCTAAGCCTT CTCCTCCNCT AAACNTCGAT 
CNTGATCGCT TGNCAAAACN CACCCCTTGG ATCTCTACTC CTCTCCCTAT 
TNNAACCNCC AATTCCGATC CNCTCCAACC TA
(break)
G GAAGAGGTTA 
CCCCCCCTTG GAGGGGTTGC CACGGGACAG CACCGCACGC GGAGGACGGG 
GAATTAAGGG GGGAGGGGGG GGGTTAAAAC AACCAATGGG GTTGAAGGGC 
TGCCGTTGAA TGATTAACCG CGGCCTGGGC CGCCTATTTG GCCGCCAAAG 
CCACAAACAC CGTTGTTGCC GAGGAGAAAG GGGAGGGAAG GGTGGGAAAT 
CACCGTTGCA GGTCCGATTT GTCGCACCGC GGGGGAAAAC TAGTCCACGG 
GGGGAGTAAC CAAAACCCAC GTAAGCGTTG TCCATCATTT AGAGCGTTTG 
ATCATGATCG CGGGGCCGAA AACCGCACCT CATTTGGATT TTCGTAGCTC 
CCTTTACCTT TATTTATAGA CCCCTCTCGA AATTTGCGGA TCCCCCTCTT 
TGAAACCCTA AATTTTCCAC CTCGCGCGCC GCCGGACAAA TGCCACCGAC 
GGACACGTCC GGATCCTCCC CGCCGCCGCC AAGTGTCGCC CCCTCAATTG 
CCATCGCCGC CTCCCTCCGC CGCC

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wmc(3c1) Plate reference : 3c1
WMC locus number : WMC Length of sequence : 1119
Last updated : Wed, Aug 20, 1997 at 2:26 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. 1500 base insert. No trimming done to insert. (CT)12 108 to 131. No room for primers.
PCR Comments :



Length of sequence : 1119  Consensus sequence is :

..

CAAAANAACA TAAAATTCTT GTTGGTCCCT CTCCCTTTCA ANTCCATCTC 
TCTCTCTATC TCTCTCTCCC TCTCTCTCCC TATCCCTCTC TCTCCCTCTC 
TCTCTCCCTC TCTCTCTCTC TCTCTCTCTC TCCCTCTCTC TCTCTCCCTC 
TCTCTTCCTC TCTCTCGCTC TCTNTCTCTC TCNCNCTCTC NCNCTCNCTC 
TCCCTCTCCC NNTCCCTTCG TANNCNAAAC AAAATTCNNG TNGGTGTNGG 
TCCCTNTCTC NNCATCNCTN TNTGNATATC TATCTCTCCC TCTNGCTCTC 
ANTNTCNNAC TCNCTCTNAN NGTNNATCTN TGTCTNGGTG ANAANGATCA 
ATTCCGGTCT CAGGCANTCA ACNANNAAAA TNGTGTTGGG GANNGGTCCC 
TTCTTCTNTA AATNTNGTNN GNNNNNTNGN TANNNATGAT TNNTNCTNNC 
NTCNACNAGA AANAAATNNN GTTGGAGGNG TAGGAGNAAA AAATNNGNGG 
GGGGGGGNNN AAGATCNNNG NATTNNGNGN CNTNCGNNNN GNNNNNTTCC 
TTGGNATNAA AAAANANATC TCNGGGGGNA AAANGGNTGG GGG
(break) 
TTTGTTTGGT CGGGGGGATA AAATTGGGGG GGGGCAAGTT GGCAATACGG 
AAAAGAATTT TCTCCTTGGG ACCGACCTGG GGGAACCCAG CCCTTTCCGG 
TTTGGAAAAA AACGGGAGGG GTTTTAGTCC GGAGGACAAC ATACCTTCGT 
ACCATTGGCG AGCTTTTGGG GTTTAGCCTC TCTAATCTCG GGGGAGATCT 
ACTCTTGGGA AAGATCAAAA CGAACTGAAA ACTTGACCAA TATTTCTATT 
GACACTGATG ACTTTGTGTG CATGCTGATG CTGATTTATT TCTGCAAGGA 
AATTAAAATT GGCAGTTCAC ACCTTACGGT TTGACATTGT GCTACTCCGC 
CTAACCCGGT TAACGCGGCA CATCATGATC AGACTCAGAC CACGTCATTT 
TTCGTGATGT CAGGACAGTG AATGATTTAT ATGGAAATAT TCCACTCCCA 
CTCACTGTGA CGGTATTGAT CGACATTTTC ATGGTGTCCC GGGTGTTATG 
TATGGTCCAT TCGCAAAAAA GAGAGA

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wmc205(3d1) Plate reference : 3d1
WMC locus number : WMC205 Length of sequence : 226
Last updated : Mar 17 Nov 1998 at 10:45
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Bases 50-161 (complement) (ie. 3' side of microsatellite) match 6f11(2) and part of 5h4. (GA)12 51 to 74.
PCR Comments : tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide. (several loci).
Forward primer details :
gcTTAgcAATAAgcTTggcAgg at 15 length 22 bases, Tm 64
Reverse primer details :
ccgTTgTcAAAgATgcATgTgT at 166 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 173 bases.



Length of sequence : 226  Consensus sequence is :

..

CAGACCTGGC GTATGCTTAG CAATAAGCTT GGCAGGAAGG TGTCCCTAAC 
GAGAGAGAGA GAGAGAGAGA GAGAGTGAAA AAAGTGTGTG TGCGTGTGTG 
AAAGAGACAT GGACAACACA CGATAAAAGT AGAGAAAAAG AATGCGTGTC 
TTATGCAAAC ATATAACACA TGCATCTTTG ACAACGGAGA AAAGGCGAGG 
AGATAGTGTG TGGTTGTTTG GAACCG

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wmc(3e1) Plate reference : 3e1
WMC locus number : WMC Length of sequence : 116
Last updated : Fri, Jul 4, 1997 at 4:35 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 116  Consensus sequence is :

..

AGGGATACTA GGCTACCGTG AGTGTATAAG TTTCATGTTT CATTCACAAT 
TGTTCGTTCA TAATGTGTGT ATCCTCTTAG GCCTACTCTA CAAATATATA 
TTTCAAGTGC GTTTAA

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wmc(3f1) Plate reference : 3f1
WMC locus number : WMC Length of sequence : 321
Last updated : Fri, Jul 4, 1997 at 4:36 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 321  Consensus sequence is :

..

TGTGAGTTGG GGCTGTGTAG GCGGTAATTT GTTTTTTCTT TTTTTTGAGA 
AATGTGTAGG CGGTAAGTGG GCCCGGTTTT GGAGATGTCG GGTCTAAATG 
GGCTTGTTAA GGGGAGGTGG CGTGTGCGAG GCCTGTTTTA GCGTGCGTGT 
AGCTGGCTGC TTCTGTTCTC GCGTTCACTT TGGCGATAGT ATASGTGCAR 
CGGCGGCGGC AGCGGCGGCT GTGGGGACRT GCTCTTGTCT TCGGTTCGCA 
GARGAGGGCG GCGATGTTCT TCTCTGCTGC GTTTGTTGTT CTCCCGGGGG 
AGGTCCTGGA SCAAGGGTGA C

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wmc(3g1) Plate reference : 3g1
WMC locus number : WMC Length of sequence : 189
Last updated : Wed, Aug 20, 1997 at 1:19 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Bases 1-107 match part of 9E4. No substantial microsatellite.
PCR Comments :



Length of sequence : 189  Consensus sequence is :

..

ATTTCATTCA GTCTGACTCC GACTTTAAAT TATACGATGA TTGATATAAA 
ATAAACGGAA ATGATAAACG TTCGGATTTT AAGCATGGCA ATCCGAACGT 
TTTTCATAGC AAATTTAGAT TACGCGGCGG CGGCGGGGGC GTCTCGTGGT 
GGGAAGCGGC TCCTCTACCG TGCTCTGTGT GTCGTCGGG

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wmc(3h1) Plate reference : 3h1
WMC locus number : WMC Length of sequence : 215
Last updated : Mon, Dec 8, 1997 at 1:56 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Bases 98-204 (across and 3' side of microsatellite) match part of 6e7.(GT)14 119 to 146. Too difficult to design primers, microsatellite motif sits within minisatellite like larger repeats.
PCR Comments :



Length of sequence : 215  Consensus sequence is :

..

CTAGGGTCCC CAATTTGCAT GTCGATAACC GACTTGCAAC TAGGGGTGTG 
TGCGTGTGTG TGTCTTTTTG TCAAGGGCTC CCCAGTTGCA TGTCAAGCAT 
CGACTCGCAA CTAGAGGGGT GTGTGTGTGT GTGTGTGTGT GTGTGTTTGT 
AGAGGGTCAC TAGTTGCATG TCAATAATCG ACTCACAACT AGGGGTGTGT 
GTGTGTCCTT TTGTC

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wmc(3a2) Plate reference : 3a2
WMC locus number : WMC Length of sequence : 383
Last updated : Mon, Dec 8, 1997 at 1:58 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite, sequence too corrupt to use.
PCR Comments :



Length of sequence : 383  Consensus sequence is :

..

GCCCGAACGC ATCCCCCCxg ACACATACgA CACTAAGgAS TCCATCTCTC 
TCTCASgACA CACASASgAC RAACRMACAG GSRSGSASGS RStGGACACC 
CCCCCCACAC ACAXASTRAG ACGCCATCTS TSTCTCACAT ACACACACAC 
AARRACTRAG ACTCCATCKT TXTCTCTCTC CTSTCTCTCT STCTCACACA 
CACACACACC CCAYACWCAC ASTAAGAXTC CATCTTACAC ACACATGCTC 
TAGGCGCAAC ATTTGTTCCT ACCACCCTTC ATCCRACCYT cACCCGTATG 
ATAAACAATC ACCTTTxATT TACTTGCATR ACATGGxACW AATTtCCACT 
TcTACTTxTA GTAGTCAGCM AgACTTATCA TCT

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wmc(3b2) Plate reference : 3b2
WMC locus number : WMC Length of sequence : 295
Last updated : Fri, Jul 4, 1997 at 4:36 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 295  Consensus sequence is :

..

CATCCTGCCG CCCACCTGGG xACGTGCACT AGTTTCGCTG ACCCTGATTT 
GTAATTTGTA ACTGACCTCC TAGGAGGATT GTATCAAAAC TCCACCTTTT 
CAATGCAATG AAATGCAAAA GCCCTTTGCA TTTTCTCGAA AAAAAGAAGC 
AATCATTATT TGCTCAGCTT CCCGAAGGGA GCCGGGAGAC CTAACCCTAG 
CCGCCTCTTG CACCGCCGCC GCCTCTTCTT CATCGCGCCA CGGCGCCCCG 
CCGCCAGCAG CCGCGATCGC ATCTCCGTCA ACCCTCCCCC TTCCC

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wmc(3c2) Plate reference : 3c2
WMC locus number : WMC Length of sequence : 313
Last updated : Fri, Jul 4, 1997 at 4:36 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 313  Consensus sequence is :

..

GACCTGGTGA TCGTTGCCGA ACCGYTCGGC GGCGGCCTTG ACAGCGTCGG 
GGGATACTAC ATGCGGCGCA TCCGGGAGGT AGGGTCCCGG CAGGTATCGC 
CCCTCGTGAA GCCGCCGGAG AAGCGCTGCC GCATCCTCCC CACACGGGTG 
CCTTGATCTC GGCCTCGGCT TCCGCCGCCT AGGATCATCG CCGGACTCCG 
GCGGCAGCGG GCGAGGCTTA GGAGTGGGTC CGGGCGTGAA GAGGGAGGAG 
AGTTTCGGAG CGGCGGCGGC GGAGAGACGG CGAGAGGCGG GGAATGCCGT 
GCACGGGTTT AGG

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wmc(3d2) Plate reference : 3d2
WMC locus number : WMC Length of sequence : 219
Last updated : Lun 9 oct 2000 at 14:43
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : Unilever comments "(estimated size = 200bp). This sequence has not been edited, although I think it is possible to see roughly where the cloning site is." . Trimmed to insert at both ends, linker sequence was highly corrupted. Consensus sequence is drawn from reverse sequnce only. Bases 48-155 match part of 4a10. Matches 5H6. (GA)12 26 to 49, (GA)19 147 to 184. Too difficult to design primers, microsatellite sits within minisatellite type repeats. Bases 78 to 109 show homology to 17d8.1 bases 74 to 105 (32 in longest run, maxScore = 50). Bases 162 to 192 show homology to 12g12.1 bases 243 to 273 (complement) (31 in longest run, maxScore = 23). Bases 188 to 215 show homology to 22e10 bases 1 to 28 (complement) (28 in longest run, maxScore = 49). Bases 51 to 117 show homology to TW5c08.1 bases 64 to 130 (complement) (67 in longest run, maxScore = 52).
PCR Comments :



Length of sequence : 219  Consensus sequence is :

..

CGACAGAACT GAAGATTGTC AGAGCGAGAG AGAGAGAGAG AGAGAGAGAT 
AGAGAGAGAG AGAGATGGAG GGAGAGGGAG AGGGACCAAC AAGGTTTGTG 
TGTTGGATGC GTGCGACAGA ACTGAAGATT GTCAGAGAGA GAGAAAGAGA 
GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGATGGAGG GAGAGGGACC 
AACAAGGTTT GTGTGTTGG

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wmc(3e2) Plate reference : 3e2
WMC locus number : WMC Length of sequence : 1089
Last updated : Wed, Aug 20, 1997 at 2:35 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Are these from the same insert? No trimming done to sequence. (CT)13 223 to 248. Not enough unambiguous sequence to 3' of microsatellite to design primers.
PCR Comments :



Length of sequence : 1089  Consensus sequence is :

..

ATATGGGTNG ANTNNCAACA TNNANCACTC NCNTNGCAAA NGAAGGGGGT 
CCACCCGGGT GGTGGCNCCG GTGGTACAAA TTATGTATGC CGCGTAGGAA 
ANTGGCGGTG GTGNTCTGAT AGGGAAGTNT AGTCGGCTCG TNGNTATTGG 
TTACGTNTAT TNATCACCCA AATNCANTAA TNGTCTAGGT ATATGGTTTT 
TCCGATGGGG CCANAGCNTN GGCTCTCTCT CTCTCTCTCT CTCTCTCTCA 
CACATCACAA TTCACATAAG TNCNNGGGNT TNNTCCACNC GCCNCCATGG 
GATCCNNNGG GACTCGACNA NNTAGCTNTC TGACNCNNGG ANTANTCATN 
GGTCATGTCC NNTTGCCTGT NCTNANATTT GTTCTTCCGC TTCACNAACT 
NCACCCCCAC TTTACTTACC CNGGAATTCT TANTTNNGTC ACCGCCNCGG 
NGGGGATCNC TCTGANATGT NNACCNTCNC CCGCTCTNNT TTGCNTTNGC 
GCTCCNACAG NGCGCCTNAC NAGTTCCGGG ATATNTAATN TTNCCACCCC
(break)
GGG GGGGGGGGAT GATTGGCCTT TTTACAAATC CACTGAGGGA 
CACCAGAAAC GGTTGAGGGG CCGAAAGAGG GAAAAGGAGG TAATCCCGGT 
TCAGGCGCCA TAACCATTTC GGGCGGGGCC CCTTTGGAGA GGGGAAGGCG 
GAAAAGGCCT TTTCGGTAAA AACGCCCCCT TCCGAAAGGG GTTCGAATTT 
GTCCAGGGCG ATAAAGGTGG GGCCAAGGCC AGGGTTTTTC CCCCCCAGTT 
TTGTGGTAAA AAGGAAGTCC AAGGGAATTC CCTTGAGGCG ACGTTGATTA 
GATCTGGGTT TATCCGTTAC CTGAGCACTT TATGCGCAGA GGAGGGGGTC 
ATACGAGGGT GCCTGTGGGT TTGACTTTGT GTCTGCCACA CACACAAACA 
AAGGAAACAC AGAGGGGAAA ACAAGCACAC GCTTACCAAA GGGACCCACC 
AAGTTAGAAA ATGGTGGTTA CGGTTTTCCC TTGGGCTAAG CCTTTCCCCG 
TCTTTTTTTT TATATAACAA TCTGGATTCA ATTACATAAA AAAAAA

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wmc(3f2) Plate reference : 3f2
WMC locus number : WMC Length of sequence : 6
Last updated : Fri, Jul 4, 1997 at 4:36 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No Insert
PCR Comments :
No sequence available for this clone
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wmc206(3g2) Plate reference : 3g2
WMC locus number : WMC206 Length of sequence : 597
Last updated : Mar 17 Nov 1998 at 10:45
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : Unilever comments "(estimated size = 700bp) sequence falls off after repeat in forward seq which is probably why I cant align them. " Consensus shown is from reverse sequence only, forward sequence showed numerous ambiguities. Trimmed to insert at 5' end. Take care to use primers close in to the microsatellite on 5' side. (CA)29 332 to 389.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
TTgTgcTcgTgAATTgcATAcc at 283 length 22 bases, Tm 65
Reverse primer details :
gccAAAATggcAgcTTcTcTTA at 493 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 232 bases.



Length of sequence : 597  Consensus sequence is :

..

TTANATNACA CATCCAAGGN AAGGAAGGAG ATTGAGGTCG GCGCCTCCTG 
AAGGCATGCA TGCTTGTCAC TGAGAGAGGG AAGGGCATCT CTTTTTTTGG 
GTTGCAATTT CCACCTTGTT GTTGGGTGAG GTGAGTCATG ATTTCTCTCC 
TCCCGCTTCA TTTTTCAGTC CTCTCTAACT TCANAAATTT CCTAATTTGT 
NTGATCTATA TGTATGTAAA ACATTATGAT TAGGATCCCT CTATTTGANT 
AATTTCTAAG TTAATGGGTT ATAATTTCTG TTTTGTGCTC GTGAATTGCA 
TACCCGGGGG TTTATTCGAT TTAGAGATAG CCACACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAT TTGGAGTAGG 
TGGCTACAAC AAATTTTATA CTATGATAAG TGTGTGGTTC AGTCAACCAA 
ATTATTGCCT TATATTTTAG TTAAATTTAT TGCTGTGATC TATAAGAGAA 
GCTGCCATTT TGGCAATAGA TGCGCGATAA AGTAGTGGTT GGTGTTGGTT 
TAATCCATTA TCAAATGTTT TGCCAATAAA GGAAAATTAC CTTCCTC

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wmc(3h2) Plate reference : 3h2
WMC locus number : WMC Length of sequence : 1056
Last updated : Wed, Aug 20, 1997 at 2:39 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. 700 base insert. No trimming done to sequence. (GT)47 81 to 174. Not enough uncorrupted sequence to make primers.
PCR Comments :



Length of sequence : 1056  Consensus sequence is :

..

AAAACGAGAA CTAAATTTCC TGTCCACCTA GCCTCTANAA NTTTGATTTT 
CTTAATTGGT TCAGCATAAA TCGGTCATTT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGCANGA ATANNCANCC CNNCCCCCCC 
TCTTCNNNGA CNNCNCCNNC AAAANNANAN NNCCGNNAAC CCCTCCANNA 
CTNCCNACAA GNCCCTNCAC CCCCAACACC CCCCCCCCTG NANTCNCCCN 
NCNAAGCAAT ACCCCNNACG CCCCNCCCNC NNCNCCCNAC CCCCCGCNAN 
CNACCTNNGC NTATCCCNCA CANCCNTANA TCTCCNNCCC NNNANCCCAN 
CTNANNTCAN NACCNCTNNT NNNANNCCTN CNNNNCANNC NANCACCACC 
NCCANNAACT NNAGCCACCC ANCCNTCNTA ANCNANNNCT NCCCCGNANT 
NCNTCCACNC CNANCACTNN GCACAANTCN CCNCCCANAA 
(break)
NGA 
GAATTCCGTC ACCGCTGAAG GGGGGATTTT TAGGGTCAGG AAAAAGTTCA 
TGGAGGGGGG GGGAGGGGAG GGAGGGGGAG GGAAGAAGTG GGTTTTTGAA 
TGGGAAAAAG GGGGGAGAGA GAAAAGTGGG TGGGTGGGCA GGGAGTCGGA 
CCGATCCCCA GCCCTTTTTT TGACCGCCTT ATCCCAATAA AAACCGTACC 
ACCTCTTAGG CGGTTTTCGA TCAGGGTCGT TTCAGAGAGT CCGCGCCCAA 
ATTTTTGCCC GAGCAGAGCG GATGCGACCG CTCATTGACG CCGGCACTGA 
AGCGGTGCGC GGCCGAGAGC GCCACTTGTG CCGCTTCCCG ATTCACGCAA 
CTGTTACGCA TTCAAATGAC ACAACGACCG CCCGTTCGTC CGTCTACCAC 
CCACATTGAT TTTCTGTTTA GTTTTCTCCA TTCTTTCTCC TCAACCTATC 
TCCATTGGAT CATTAGTGTC ATCAGTTCAC CCAAGATCAG CGGGACAAAT 
AACTACTAGG TTT

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wmc207(3a3) Plate reference : 3a3
WMC locus number : WMC207 Length of sequence : 314
Last updated : Mar 30 mai 2000 at 9:54
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Bases 151-284 (complement) (ie. 3' side of microsatellite) match part of 6F4 (the reverse primer shown is not included in this region of homology). (CT)32 57 to 120. The primers have very high Tms. If they give a smear try decreasing the length. Bases 198 to 314 show homology to TW3d07.2 bases 47 to 163 (117 in longest run, maxScore = 72).
PCR Comments : 61C anneal, product ( beware primer dimer).
Forward primer details :
cTcccTccTccccTgcAAcAcc at 35 length 22 bases, Tm 72
Reverse primer details :
ggcAgggcTTcgccgAgcTTcT at 123 length 22 bases, Tm 75
Expected size of amplified fragment (Chinese Spring=) 110 bases.



Length of sequence : 314  Consensus sequence is :

..

AGGGAGGGAG GGCAXCAACC TACAGCCTTG GGCGCCTCCC TCCTCCCCTG 
CAACACCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTCTCTCTCT CTCTCTCTCT CGCAGAAGCT CGGCGAAGCC CTGCCGGAGA 
CCCGCTACAT CCACCACCAC GCCGTCGTGT TGTTGGATCT CCATCAACCT 
CTCCTTCCCC CTTGCTGGAT CAAGAAGGAG GAGACGTCGC TGCACCGTAC 
GTGTGTTGAA CGCGGAGGTG CCGTCCGTTC GGCACTCGGT CATCGGTGAT 
TTGGATCACG GCGA

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wmc(3b3) Plate reference : 3b3
WMC locus number : WMC Length of sequence : 281
Last updated : Wed, Aug 20, 1997 at 2:44 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Matches 8G10. (CA)10 260 to 279. No room for primers.
PCR Comments :



Length of sequence : 281  Consensus sequence is :

..

CAGCGGTGGC GGCGGCGATC TGCGGCGGCT ACGACGACGG CGACCTCGAT 
CACCCCGATG GCGGCGACCT GGTAAGATCG GCTACTACCC TACGTTCCTT 
CGACCACCTC CTGCTCCTGA ACGTACGGAG RAAATCTAAA AAAATCGGAG 
ATTACATTCA CACTTCATAG TGAACGTACA GACTGAAGAC ATACTGAAGA 
ATATTACAGT GAGTATTCTT CTAGGAAAGT TTCATCACCT TTTATTGTAA 
TCACAAAGTC ACACACACAC ACACACACAC T

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wmc(3c3) Plate reference : 3c3
WMC locus number : WMC Length of sequence : 189
Last updated : Fri, Jul 4, 1997 at 4:37 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 189  Consensus sequence is :

..

AGCGAGTCAT CGTGTGTGTG ATAGGGACTT CATGAGAGAT CTAGAATAGA 
CGGTGCAGAA AACAACGTGC GAGATTGAGA ACGATGATAC ATTAGGGAGM 
TATGGGAAGA GTCACGGCAT ATATGTATAC AGGGAAGGAG CAGGGGCGGG 
TCTGCATGTG GGAGAGATAG AAAGAGGAGA GTGGTGCAC

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wmc(3d3) Plate reference : 3d3
WMC locus number : WMC Length of sequence : 741
Last updated : Mon, Dec 8, 1997 at 2:43 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. (GT)22 5 to 48, (GT)18 430 to 465. Insufficient uncorrupted sequence 5' of second microsatellite to make primers.
PCR Comments :



Length of sequence : 741  Consensus sequence is :

..

AAATGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTTT 
TATGGGTTTC TTCCATAGGT GTGTGTTCGT GAGAATCACA AGAGAGCATT 
GAGAGGAGAA GCGTTGCGAG GAGAGGCAGC GCTAACAGCC GTGTTGCCAG 
AGTGGCCGTC GATGGCGCTG TTGTGCAXCA CCGAGATGAG TTCTGTCTAG 
AGCACCGTTT GGCRYSWAKS KWYAKYCRAS CCKRYYGKYG KMKMWYRSCC 
WYTYGKWRKW MGGGKTGGKG GCTCCAACSG GTTAGTCCAC GSKKGRAYWA 
GSSRWYCCCC CGGCGAGAAA GgAAACGGTC GAAACGCAGA CCASTTTTGA 
CTTTGAGTCS AGTGGGGAAG GGgATCAATC MCCcGCTCGC CASGgTTTGT 
ATGCGGWGTG gCTTGCTAAR AAAYCYCxtg tgtgtgtgtg tgTGTGTGTG 
TGTGTGTGTG TGTGTGxgTG TSCgtgtgtg tgtgtgCgTG TGTGTGTGGG 
TGTGTSTSTC YSYGTGGGGG GGTKGGGGGC SCAGgATgTC MGTTTTARTG 
TTTMCgTYCC SYgCGAMCCA TTTATGTTTT TTCTCCGAAA TGATAACATA 
AACTTATAAA CTTGAAAGTT TACAATTCAA CAAAACAAAA CAACTTCCAT 
GTTTTACAGA AAATTCCAGT TTCTTGATAC ACGGTAAATA TCTAAACTGG 
GATTCTTACA ATCAAGCTCT ACAATTTGAA GTTCATATAT C

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wmc208(3e3) Plate reference : 3e3
WMC locus number : WMC208 Length of sequence : 899
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : unilever comments "(estimated size = 600bp)" No overlap between forward and reverse sequences. No trimming done to insert. (GA)8 825 to 840, (GT)27 285 to 338. Bases 837 to 892 show homology to 26d3 bases 66 to 121 (complement) (56 in longest run, maxScore = 47).
PCR Comments : tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide. (several loci).
Forward primer details :
ATTgccTTTggAcTTccTcAcc at 672 length 22 bases, Tm 65
Reverse primer details :
cAcAAgcTTgcTTcTTcTTcAT at 845 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 186 bases.



Length of sequence : 899  Consensus sequence is :

..

ATAATTTCAT GAACGTCAGC TTGTCAAGTT GTGTGGCACA GAAGATTGTT 
AGTTGGTCTC AGGAAGAAGA AGCATAGAAA GGTGGTGATC AAGATAGATT 
ACCAACTCAT TGCACAATAA CCCAATTCAT AACCAAATAA GCTAGCACAT 
TGATATGTAT TTTCATTAAT AGATAGTGCA TGGAACAATT GAGGTCCAAC 
GTGTATGGAC CAACGACTAG CCTGCTGACT TCCTCACAAC CCTCTACGAG 
ACAGAATTAT TGAGATGCAG AACCGTGATA AGAAGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGA GAGAAAAAGA 
GAGAGANGGA GAGA
(break)
GGCAAAACG GTTTTCCCCC TCCCCCCCTG 
GAATTAGACC CCCCGGAATT CTTGGAAGCC TCGGGGGAAA AACCATTGGG 
GAAATTTGCA TTTTGGAGTT AGGGGCAACG CAAAAATTGT TAAAAAATTT 
TCCGGGAAGC CGAAGGCCCA AAAAAGGAGG GAAGTCCAAG AAAGTATTTA 
CCCAATTCGT TGCACCGATG GCCCCCCATT TCAGGAAACC CCGAAAGACC 
CGAAGCCCCC CAGATAAGTT ATTTTCAAAC AAAGATGGGG GCCGGGGGAC 
CCTTGGAGGT CCAACGGGTT TGGGCCAACG AATTGCCTTT GGACTTCCTC 
ACCACCCTCT ACGAGACAGA ATTTTTGAGA TGCAGAACCC TGATTGGATT 
TGTGTTTGTG TGTTTGTGGA AAAAAAAAGG GGGTGTGTGT GGGTGTGTGT 
GTGTGAGAGA GAGAGAAAGA GAGGGAGAGA GAGAGAGAGA TGACAATGAA 
GAAGAAGCAA GCTTGTGAAG GGAAATGTTA GAAAAAATCG TTGTTCAAT

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wmc209(3f3) Plate reference : 3f3
WMC locus number : WMC209 Length of sequence : 664
Last updated : Mar 17 Nov 1998 at 10:46
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. bases 475-650 related to 8h9, bases 112-320 (ie 5' side of complex microsatellite) match part of 11h2. Forward primer is within this region though has 2 mismatches to 11H2. (CT)11 604 to 625, (GT)4G(CA)2(GT)6(CT)(GT)4(AT)((GT)2 at 322-362. Bases 425 to 442 show homology to 17d10 bases 95 to 112 (complement) (18 in longest run, maxScore = 57).
PCR Comments : 51C anneal, product.
Forward primer details :
ATAAccTTgggATgggggATgA at 275 length 22 bases, Tm 67
Reverse primer details :
cTcTgTAggTTTgATTTcTcTc at 369 length 22 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 116 bases.



Length of sequence : 664  Consensus sequence is :

..

ATGTGGAAGG AGAAGAGACA ACGGGTTTGA TGAGAGAGCT GGAAAAACAT 
GGATGAGAGG GGAAAGATCT CGTGGGTTGA GGGATTGATA ATATTGTGCG 
GAAGAGAGAG AGGGGGAGGA GCCAGTCAGC AGTCCTCACA TCATCCTGCT 
TCCAAATGGC CCCGCATTAT CTAGTGCAGT CTGGTCGCCG TCTTCGATCT 
CCTCGATGCA CTCTTTGAAG ATTGAGGTGG TGTGCATGCT GGGTGTAGAG 
AATCACARGC GAGAGTGKTC GCCATATAAC CTTGGgATgG GGGATGAATT 
GTGTGCTCSG GGGATGGGGG AGTGTGTGTG CaCagTGTGT GTGTGTCTGT 
GTGTGTATGT GTCAGATATT GAGAGAAATC AAACCTACAG AGAGAATGTG 
TGTGCATGTG ATGGAAAGCA ACATGCTAAA TAGGGTTTTC MCTAASAGAT 
TGTGTGTGCG AGAWARARAg CRAtGTGCAG GCCTTGAGGC CGGCAGGGgC 
CGGGCGAGGG TGTCAAACTG TGTGTGTTGA TGCATGTGTT ACATGCGATT 
GTGCGAGGGA GGGACGAATT GAGAGAGATG GTTGTTGTGT TACGGATGCT 
CCCCTCTCTC TCTCTCTCTC TCTCTCGTGC ACGCGCACGC ACACACACGC 
ACAAGTTAGT CCAC

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