Wheat microsatellite WMC 25/06/01

wmc(3g3) Plate reference : 3g3
WMC locus number : WMC Length of sequence : 357
Last updated : Fri, Jul 4, 1997 at 4:38 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 357  Consensus sequence is :

..

GAAAGCAGGT CCTTTCCGCT CATGTCTTTG GTCGGGGTAG CGGCTAATCT 
CGTGTGAGGA GGTGTCAAGG TCTTGGATGT CGGGGTGGCG GCCCTGATGG 
TGGCTCCGCG GGGCTCTTGG GCAGAGCCAG TGGCTTGGTG TTGCCCAGTT 
GCCATGGCCG TGTGGGCGGC GTGGTAACCG GGGTGTGGCG TTCGGCGGCG 
GTGAATGGTG GCCGGGGTGA AAACCTGCTC TTTCTTCGGA TGGACCGGCG 
GCGGGGATGG GGCCCTTGAC CTCCTCGGTG GCGGGCTTGA CGCCGTGGAT 
CTCGAGGGTG CCGGCGATGA TGCGGAGGGA CTCGCTGAGG ATGGCGATGA 
AGGAGTC

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wmc(3h3) Plate reference : 3h3
WMC locus number : WMC Length of sequence : 885
Last updated : Fri, Jul 4, 1997 at 4:38 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : Unilever comments "(SIZE = 367bp from for seq. rev nseq not very good after 340bp)" No overlap between forward and reverse sequences. No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 885  Consensus sequence is :

..

GGANACACTC AAGTAGGATT GGAGCCCGGA AAACCAACTC GAAGGTGGGA 
CGCCGCTATC CACCCGAATG TCGACGATAC GAGAAAAACC CTAGTCTAAA 
CTACTAACCG GGATTCCCCT ACCCGTCGCC TACCGCCGAA ACTGCAGGGG 
AGGGGGATGC TCCATGGGTT GGTCGGTGAA GTCAGAAGGG ACTAGATCTT 
AAGCCGCCGC CGCCGCCCTT GTGTGGAGCA TAGAGCAAAA TCAACATANG 
CACAATCGCT ATATTGCATA TGGAGAACAC TCCATTCCGT TTTATGACGG 
TTANTATAAC CACCGACCTG ATGTATCCAC TGTGNCTAGA CTTGCTCTTA 
TGACACCGGT CNTCTTC
(break)
CTGTGG AAGGCGATGG GGGGCTTTTT 
TTAAAGCCAG CCGGGAGGGG GAAGTGCTGC AAGGGATTAA GTGGTTAACG 
CAGGGTTTTT TCCCCTCAGG AGGTTTTAAA ACGAAGGCCC GGGAATTCTT 
CGAAGGGGGG GGGACCAGGA CACACTTAAA GTTGGATTGG GGCCCGGGAA 
ACCCACTCGA AAGGGGGGAC GCCGCTATCC ACCCGAATGT CGACGATACG 
AGAAAAACCC TAGTCTAAAG TACTTACCGG GATTCCCCTA CCCGTCGCCT 
ACCGCCGAAA CTGCAGGGGA GGGGGAAGCT CCATGGGTTG GTCGGTGAAG 
TCAGAAGGGA CTAGATTTTA AGCCGCCGCC GCCGCCCTTG TGTGGAGCAT 
AGAGCAAAAT CAACATAGGC ACAATCGCTA TATTGCATAT GGAGAACACT 
CCATTCCGTT TTATGACGGT TATTATAACC ACCGACCTGA TGTATCCACT 
GTGCTAGACT TGCTCTTATG ACACCGGTCA TCTTC

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wmc(3a4) Plate reference : 3a4
WMC locus number : WMC Length of sequence : 230
Last updated : Mon, Dec 8, 1997 at 3:23 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Member of 1g1 family.(GT)10 144 to 163, (GT)8 165 to 180. No room for primers.
PCR Comments :



Length of sequence : 230  Consensus sequence is :

..

AAAAAAAGTG GAATTTGTCC ATGTTATGCA ACTAAATAAA GGTGATTGTT 
TATCATACGG GTAAGGTTGG ATGAAGGGTG GTAGGAACAA ATGCTCCGCC 
TAGAGCATGT GTGTGTGAGA TGGAGTCTTA STGTGTRTGT GTGGTGTGTG 
TGTGTGTGTG TGTGGTGTGT GTGTGTGTGT GAGAGAGAGA GAGATGGAGT 
CTTAGTTTGT GTGTGTGAGA GAGAGAGATG

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wmc(3b4) Plate reference : 3b4
WMC locus number : WMC Length of sequence : 291
Last updated : Mon, Dec 8, 1997 at 3:23 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. (CA)13 260 to 285. No room for primers.
PCR Comments :



Length of sequence : 291  Consensus sequence is :

..

TGAAACACAA ATAGTGGCAT AAATGATAAA CCCCTTTTTT AAACATTCTC 
CTCTAATTGT GACTCACTTA CATGCTTCAC TGTTGATACG TAGGTTGACA 
ACCCCAAATG CTTGTTTGAC CTGTTTAGAT TTGATGTTTC GATGGATTGT 
ATATAAAGTT ATGAACAATA TGTTTTCTCC TCTCCATTCC CTTCCCCACA 
ATTACCTTAT TCCGATAGTG CAATTATTGT AATGTCATAC ATCTAGCCAG 
CTAGATGGTC ACACACACAC ACACACACAC ACACATTCCG A

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wmc(3c4) Plate reference : 3c4
WMC locus number : WMC Length of sequence : 428
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Matches 5e11. Complex microsatellite 13-59. No room for primers. Bases 74 to 91 show homology to 19c5 bases 361 to 378 (complement) (18 in longest run, maxScore = 27). Bases 65 to 158 show homology to 25g9 bases 4 to 97 (complement) (94 in longest run, maxScore = 77).
PCR Comments :



Length of sequence : 428  Consensus sequence is :

..

GCTTGCGCGC GCGTGTGTGT GTGTGTKTCT GAGAGAGAGA GAGGGAGAGA 
GAGAGAGAGT GAAGTCTCAA AACAAAAGGT GCTCTGCTGG AATCCCATTG 
TATGTATTCA TATGGTTCCG GAACTCGAGT TAACCTTAGG CATATGTGTG 
AGAGACATAA TTATACTTTG AGACATTAGT GGCTGTGGGT GGGCGGAATT 
AAAGTGGTGG GCGTGTTCAA CAGAGAGAGC GTGTGTGTTT CCGTATGAGA 
GACTTGTAGG ACGGGGTAGA AAGACGAATT CTAACCTTGA CGGAGGGAGA 
GAAATACACG AAGTGCATGT GTGTGATTCC GATGCCCACA GGGAAATGTG 
ATATGTATGC GTGTGAGAGA GATTGCACAA TTCATTCACG TATCACTATC 
TAGCGATCGT GGACATGCAT CGCTTGTT

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wmc(3d4) Plate reference : 3d4
WMC locus number : WMC Length of sequence : 1198
Last updated : Mon, Dec 8, 1997 at 3:27 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : Unilever comments "NO ALIGNMENT (MS RIGHT NEXT TO CLONING SITE estimated size = 300bp)". No overlap between forward and reverse sequences. No trimming done to insert. (GA)10 4 to 23, (GA)9 1119 to 1136, (GA)17 1141 to 1174. Sequence evidentally highly corrupt. No room for primers.
PCR Comments :



Length of sequence : 1198  Consensus sequence is :

..

AAAGAGAGAG AGAGAGAGAG AGAGGGAGAG AGAGANAGAG CGAGAGGGAG 
AGAGAGAAAG GANGGAACCT NACATTCTTC TCTNATNGNN CNCACCNTTA 
GNCNTATATN NTTNNTTGTC CCCTTCNTTT NCANTTCCTC TNNNTCNTNN 
TNTNGANCNT TTACTTANTG NTACCCNCTT TTNTTTNTNT TNNNNNGACC 
GCTTCGCATC CCCTCTTNGN AGNTGACNCN CNCCCGTANT TTCGCATATN 
CGTNNCCTNC TAATGCNNNG AGTNTAGNGG CCCCCCTTCN NGCTGGCACC 
TTCCCCNNNG NNNGTCGTTT TTNATNCNCN NNGNNNAGAC TANCATTNNC 
CCNTNNNNCC CACTGTTACG TAGTNTCAAN CNATNTNNTN NATCTTNTNG 
NTTTCCNTGT NATNCACCCC NGNNGTNGAG TGATCNGCTC GTCCCNNNCG 
CANNNNCCNC NCCGTNCCTA CNNNTTTTNN TTTGCNTCNN NTGNNTTNNG 
GTTTTTGNNA NGANTNGGGN NNTGTNNTNG GNTCTCNNNC TNNTANGNNN 
NCTNAATNNN NCNNNCCNTC NGNNATTTAC ANNGCANTNG ANTGCTNCAG 
CTNNNNTNAT ANCNNCCCGC TNCATN
(break)
CCCGGCC TTAAAACAGG 
ATCACCCTCC CTCCCCCCAT TAGAACCCCC CTTTCGGACC CAATCCCCCA 
AATTTTCCTA AAAAAACCCC CTTCCGGTCC ATTCCCTTTC TACTACTCCC 
ACTATCGAGA AACCCCTCAA ACTCTTCCTT TTCCTTAAAA CCTCCCCCTT 
TTGGTACTGT AATTACTCCC CCCCCCCTTT TTTGAAAACT AAAACCCGGG 
GCTTTTCCCC CTTTTTACAT TTTGTTAAAC CCCCCTCCCC CTTTTTTTTT 
TCCATTTCCT GTTTCAATTG GAGGGAAGGG GGTTCAAAGG GTTCATTTGG 
GGCTACCCCT ACTCCCCAAT CCGGCCCCTT TTTTTTTGAA AGATTTTTTT 
CACAGACCCA AAACTACAAA TTTCCCCCAG AAAAAAACAG TCGGAAAGGG 
GGCGAGCGGC AGAAAGGGGG GGGGTAGAGC CAAAAAGAAA AAGGGAGCGA 
TGGAGAGAGA AAGAAAAAGA GAGAGAGAGA GAGAGAGTGG GAGAGAGAGA 
GAGAGAGAGA GAGAGAGAGA GAGAGGGAGG GATAGGGAGA GAGAGAGA

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wmc210(3e4) Plate reference : 3e4
WMC locus number : WMC210 Length of sequence : 1117
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. No trimming done to insert. Complex GAA and GA microsatellite. Bases 881 to 904 show homology to 10f8 bases 196 to 219 (complement) (24 in longest run, maxScore = 81). Bases 904 to 912 show homology to 2b5 bases 113 to 121 (complement) (9 in longest run, maxScore = 20).
PCR Comments : tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide. (several loci).
Forward primer details :
gccAAcggccTTTgTTgTTAgT at 765 length 22 bases, Tm 66
Reverse primer details :
TTAAcggAgcgTTTggATTcTT at 1017 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 274 bases.



Length of sequence : 1117  Consensus sequence is :

..

AAAATACGCC CAACTCNCGC CACTATTCAC ATATGCATGT GCATCCATGN 
CGTGATGATG TTTATGCTAT GGATATGAAT CTGACCTTCT CCTGTGCGAT 
GGAATCTACT TCTTGGGTGC NACACATCTT ATGCTGCCGG CNATGGGGTT 
GTGCCTGCGG ACNATGGATN TCACTGGCGG CGGTGGCGGC GGCGGGCTTT 
GTGGTGAACA ACAANANCAA GAACAAGAAG ACNANCAAGA NNACNATGAN 
GANNAAGAAG AANANCCAAG ACCAANANNA NNANGANNNA NNAAGAACAN 
NANCAAGANN AANTANNAAC ANGACNACGG NGGNGGANAN CCCTCCCNCC 
AAGGATTNCC NACGCATGAT TCACTNACNA NCCNTNCACN NCTNNCNACN 
NCCACTNCGA CTAACAACTN CGNCCNCCAN NNCNANNNCC TCCTCNANNC 
CNCCCCGCTC CNNNTCCTNG NCTNCNGTNT CACNTCNNCN CTCNNGGGAT 
NGAGGTNNNN TTTNCCCCCT CNTTNNNCNT TANATTTCNC GNCCCCNCCC 
CAGGTNCNCC CNTCCCCGNG GCTCCTT
(break)
ACCCAC CCCCCATAAA 
CAAACCCATT GCCTCCCGGG AGAGAATGAA CCATTTCTCC AGGGGGAAAT 
TTTCCCTTTC TCCTTTTGCC AAAAAATGTT TTTGTTGGCG CCCAAACAAA 
TCTAATTCCG CCCCCCGAGG GGAATATTCA CTGCGCGAAG CCGGAAAAAC 
ACCCGCCGGG CGGTAGACCG CCAACGGCCT TTGTTGTTAG TAAAAGGAAG 
AGAGGGGAAA GGCCGGCGTT GTATGCAGGG AGACCTAGGC GAAGAAGGGG 
TCGAAGCAAT AGAAGGAGGG GTAGAAGGAG GTGGAGGAGA AGAAGAAGAA 
GAAGGAGAAG GAGGAGGAGG AGGAGCAAAA TCAGAAGAAG AAGAAGAAGA 
AGGAGAAGGA GAAGAAGAAG AAGGAGAAGA AGAAGAAGGA GGAGGAGAAG 
AAGAAGAAGA AGAAGAACTC CAAGAATCCA AACGCTCCGT TAATTGATAT 
TTGTAATAAT TTGGAAATGA AGGGACAGAG GTTCTTTTTA AAGCACTTCT 
TTTTGCATGA AAATATT

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wmc(3f4) Plate reference : 3f4
WMC locus number : WMC Length of sequence : 329
Last updated : Fri, Jul 4, 1997 at 4:39 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 329  Consensus sequence is :

..

MXATCGTGGG CTAGAAATGA TGAGGCTGGC CAAGGCCCGC CTCGCTGCCG 
ACATGGCGGC GGCGGCAGAT GCGGCGGCGA TGCATCCACG TAAATTTTTA 
CGTACGTTTA CGGGCTCGTC TGACATGGCA TAGTTTGACT GGAGTAAGAG 
GGAGGGTGAG GCCCACCCCG CCTGAAAATC AGTGGGGAGA GAGAGAAATA 
GAGAAAGGTT ACGTAAGGCA ACATGTAGCA GTTCGTAGGT CTAGTATTTT 
CGCCAACAAT AACAGGAAAA ACACTAAGTG TCATGATATT GTTTAAAGGG 
CAATGCTACA CTTACGTAAA GATTGTTAC

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wmc211(3g4) Plate reference : 3g4
WMC locus number : WMC211 Length of sequence : 427
Last updated : Lun 30 oct 2000 at 16:59
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Bases 163-337 (ie 3' side of microsatellite) match part of 6d12. Reverse primer is within a perfectly matched section. (CA)15 135 to 164, (CT)11 113 to 134. Bases 112 to 135 show homology to TW7b04.2 bases 97 to 120 (24 in longest run, maxScore = 54).
PCR Comments : 61C anneal, product.
Forward primer details :
TcAcAcTATATcggccTcTccA at 54 length 22 bases, Tm 64
Reverse primer details :
gAAcgTTgcAccTTcTcAccTT at 203 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 171 bases.



Length of sequence : 427  Consensus sequence is :

..

TACTCCGCTA TATAGGTAGG AGCCTCCGCG CTTGCTTGCT AGTGTTGCTC 
TCTTCACACT ATATCGGCCT CTCCAGATCT AAACATGACA GCGGTGGCCA 
CTCTCTCTCK GTCTCTCTCT CTCTCTCTCT CTCTCACACA CACACACACA 
CACACACACA CACACCGCTG GTCACAGATC CAGCCGGATC CTCTCCGCCG 
AGGAAGGTGA GAAGGTGCAA CGTTCACGCG GTCATCCTCG GCAACGGCTC 
TTACCCACTG CTGGGCGCGT CCCGATCAAC CTGCCTTGCC GTTCTCTCCC 
AAGCACCGCT GGCGAGGGAG GGCCTCCAAC CCCTTCTTCC TCGTTGCCGT 
AGTGTGGGCA GATCCGGCTT CCCGCCGCCC ACCTAGCGAC ATCCCGGCGG 
SCATCAAGTA TAACTTCCTT CGAAACC

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wmc(3h4) Plate reference : 3h4
WMC locus number : WMC Length of sequence : 140
Last updated : Fri, Jul 4, 1997 at 4:40 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 140  Consensus sequence is :

..

GACCCACATG AGACACGAGA CGCCGTCTTC TTTCAAGCCA TACTATTTCT 
CGTAGGAGTT CGCGATCTCA TCGGTGGAGA CTCGAGCGAC AACCGGCAGG 
AATGGAGCGG CGGCGGATGA AAAGCTAGTA AGACTGGATC

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wmc(3a5) Plate reference : 3a5
WMC locus number : WMC Length of sequence : 186
Last updated : Mar 20 Juil 1999 at 16:57
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : Sequence trimmed at Unilever. Part of 1g1 family. No substantial microsatellite. Bases 97 to 137 show homology to 26d7 bases 91 to 131 (41 in longest run, maxScore = 28). Near duplicate with 26d7, 3a5 has priority.
PCR Comments :



Length of sequence : 186  Consensus sequence is :

..

CACACACTAA GACTCCATCT CTYTYTYTCA CACACACAAA CACACACACA 
CGCAAGCATG CCCCCCCCCA CACACACTAA GACTCCATCT CTCTCCCTCT 
CTCTCACACA CACACAAACT AAGACTCCAT CTCTCTCTCC TCTCTCTCTC 
TCTCACACAC ACACACTAAG ACTCCATCTC ACACAC

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wmc(3b5) Plate reference : 3b5
WMC locus number : WMC Length of sequence : 250
Last updated : Lun 28 DŽc 1998 at 15:19
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. (CA)13 29 to 54. No room for primers. Bases 32 to 119 show homology to 21b12 bases 523 to 610 (complement) (88 in longest run, maxScore = 101). Duplicate with 21b12, 3b5 has priority.
PCR Comments :



Length of sequence : 250  Consensus sequence is :

..

CACCCGCGAA CACACACACA CGCACATACA CACACACACA CACACACACA 
CACAACGCCA CCGCAGGCAA GATCTAACAA ACAAGCACAC ACACAACAGA 
CGGGTTCAGC TGTGGGCACA ACACAACAAG CCCAACACAC AGACACACAA 
CCATGCACAA TGCAAACGGT GGCAATGATG CGTGGAACAT CTAACCCGGC 
TCCCGGTGGT GGTGGGTGAA GCAGCGGAGC CATGCCGGCG AGCCATCGTG

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wmc(3c5) Plate reference : 3c5
WMC locus number : WMC Length of sequence : 729
Last updated : Fri, Jul 4, 1997 at 4:40 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 729  Consensus sequence is :

..

ASCMTSAGKT KWKRRRSRRA ARWMYRTAWK RRRWMGYWMK GWGTGTAKKK 
TTYASRSATR XRKARRRAWK KKGGSCGYCK CMYMMWWAAW MARTGCRKGM 
WRAAAAMWWW WTAGKTGGTC CTTMAKGAAt CCGAWCCAtA GRAAAKGaGT 
TAGcGACGaC CTTGCCATCA TCGGGGRGTT MtGCACAYGC CgCASCCGCA 
GATCGAGAaG KTCgCGTtGG GgAGAYTCAG ATCCGGCTAC CCTATgGGAT 
CTGTTGTTtC CAGSCCAGCG GGTGGgATTC GGATCYTCCG CCGCCSGGCA 
CGGCAGCAGC AACACGTGGA GGAGAAGTAG CAAAYKTCCT GGGTGGACAT 
GGGCGCAGGA GAACGARCTC SCCGCCGcTG CAgCACGCTA GgCAAYTGTC 
GCGgCCACAG ASTATGGGAA xGGAGRASgC CAMCAAGGCA GGGGCRAKKT 
CAAgTTGARG TKCGARCMAC MCCSCWYCSG GAaTRATRAA GRARAGSCYG 
YKWSWCYCMA RGKWRRGCCS SSYKTKKGTK TTTRGRGRGS XSSGSSGSAG 
AMWWKTTGWW GWGRCSMCRY SCWYCTYYSY CYCCWYYMYC TCCWYCYGCK 
RYCWYSCARY MKSCRRAWMR GKMRMAARGR TWAACGRRRR RTTMAACGGS 
WRRSMYSCSS MSGXWWGGKC KCGCGGAARR GRWKGRTYSG CGGSGGSGMM 
SRRRKKKWYC SSCSSYRRSG RYMSCGAAK

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wmc(3d5) Plate reference : 3d5
WMC locus number : WMC Length of sequence : 268
Last updated : Wed, Aug 20, 1997 at 1:44 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Matches 8d7. No substantial microsatellite.
PCR Comments :



Length of sequence : 268  Consensus sequence is :

..

GCCGCCGCAG GTCCTTGCCG CAGCTGCCGC GTCMTATCCT CCATGAGCCA 
CCGCCGCAGG TCCTTGTCGG CGCCGCCGCC ACACTMTATC CTCCGTGTGC 
CGCCGCCGCA TGTCCTTGCC GCCGCCGTGT CATCCTCCTC GCGCAACTGC 
TGCCGCTACT CTACCTCATG CACTACTGCT GCATCCTTCG CAAGCTAGCC 
AGCCCAATTG CAAGCTAGCC AGCCCGATTA GAATCCATCA AGTTGTGTGC 
ATGTCTACTT GCTAGCTA

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wmc(3e5) Plate reference : 3e5
WMC locus number : WMC Length of sequence : 664
Last updated : Fri, Jul 4, 1997 at 4:40 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite. Bases 315-460 match part of 3d12.
PCR Comments :



Length of sequence : 664  Consensus sequence is :

..

MGYGMARASW KGMASRMCGM TRSSGKSGSM RRSRCGGWCW SGAXMCKMAC 
CTKCCMSKKS RGMMSRACKM MSMWSCWYYC RSCRSMGCSG MSTYYSRYSG 
CCCMGMWKAS GTSSYCMSMS AKKSTKSYCY TRTKSWGKWK YGTKCMRKKS 
RCGYMYWSMR WCYMYRMWTC KRYWWKRTAA TSWWWKSMWW KSYMGYSCKT 
GCKYRTSATY WTGRCSSGRA RCCXCCGCCG MTAAAATGgC YTCSGACSCG 
gACGKTcGGG CACACgCCGG STxGGGAWTC CCAGRAGGXT GYTYGCCTTY 
GAWATGGSAT AGTAAgAGYT CGCCGAGCGT CGCGCAKGAC TCCACCCACG 
CTGTMGAcGT CTGGTTGTCG ACCACCACGG CGCGTAGCTC CGATATGTCR 
AgCATGGCGA ASCCxTCGGT GTGCACGGAC cGGTTAGTCC ACSCGTGGAC 
TAACAGAGAR GACRAGgTCW TAcTGSCATx GXCxGATSCS xTCCcGgTGG 
TTGCCGCCxC XgtCTTGGAT CGATYCgCAG TAGAgCTCAG GTGGTCGCCG 
xCxGTCgCCG CCGCCGATSC RAKTGGGRAA gAGGGgAGAR ARCTGTAWGG 
AGGgAAATGT KAGGgTAWTT TaTRACRACK TgTGTGgAAA CAaCgCRACg 
TCTCGGGRAC GCaC

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wmc(3f5) Plate reference : 3f5
WMC locus number : WMC Length of sequence : 618
Last updated : Fri, Jul 4, 1997 at 4:41 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 618  Consensus sequence is :

..

GCCKKWTTYT YWAAGAGTTY WTWYTAACAa CATKGCAaTS ATGYTCATCA 
TTCRAAGATT TAGtGCCAAA ATTTTWAATG MATTCTTCYT CTAGCMATTG 
AGCACMATTA TCGGAaTCCT TATTCtTCAT AAAAGATATT AAAAAGATGA 
AGCATATGAG GCACCYTCAA TTCCATTTTT TKGTAGTTTT CTTWGATAAA 
CTAAACTAGT GgATAAAACA AGAAAWcTAA AAGATTCGAT TGCAAGATCT 
AAAGATATAC YTTCAAGCAC TCACYTCCCC GGCAACGGCG CCAGAAAAGA 
GCTTGATGTC TAATACGCAA CYTTCTTCTT GTAGACGTTG TTGGGCCTCC 
AAGTGCAGAR GTTTGTAGGA TAGTAGCAAA TTTCCCTCAA GTGGATGACC 
TAAGGTTTAT CAATCCGTGG GgARGCGTAG GATGAAGATG KTYTYTCTCA 
AGxCAACCCT GCAACAGAAT AACAAAGAGT YTCTTGTGTc CCCAACACAC 
CCAATACAAT GGTTAGTTGT ATAGGTGCAC TARTTTGGTC MAGARATGGT 
GATATAAGTG CMATATGGAT GGTAGATATG AGTATTtGTA AtCTGAAAAT 
aTGAAAACAS CAaGGTAK

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wmc(3g5) Plate reference : 3g5
WMC locus number : WMC Length of sequence : 1154
Last updated : Mar 30 mai 2000 at 9:54
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : Unilever comments "estimated size = 750bp)". No overlap between forward and reverse sequences. No trimming done to insert. (GA)24 874 to 921. Sequence is evidentally highly corrupt, too difficult to design primers. Bases 964 to 988 show homology to TW4f09 bases 195 to 219 (25 in longest run, maxScore = 28).
PCR Comments :



Length of sequence : 1154  Consensus sequence is :

..

CCGATGCANC GGAAGCANAC GCAACGTCAA GGTGAGCTCC CNCGCGACGG 
CAGACNGGGC CTANGCGAGC GGAGTTGGTC GAATCTGGGA CGGAGGTGGC 
GGGCTCGTCT GGTTCCCNGC CGGATNCGGT CCGGNGGGAC TCCATGGGGT 
GGCGGCCATG GCGTCNANGC TGCGGCTTCC TGNGCTCNGA TAGAANCGAG 
AGANAGAGCN NTGANACAGA NACANACANA NCCTCATNAC TCCCGGANTC 
CCCAGANANN CANANAGANA CAGANACANA CACANANAGA CACANANANA 
NANAGANACA TNGANANNCA NANACNTAGA TANATGGCCG GGGNTNANNC 
ATNGGCCTNT CCCNTGTGTT CNTCNNCNCC CCNNGGNAGG CGAAANTCGN 
TCNCCCCCCA CGTGNATCNA CTCCCCCCTN NCGACNTNAN GGCCNNCCTC 
CCCCGTCTNT NTNCCTCGGC GNTTTTCNTA TTTGCCNGAC NNNCNCCTCT 
CTCNNNNGCC ACATATCTNG GACTCCTCTT CCCATNTACA AACNTCTCTN 
NACANNCNCT TTNCTCNCCT CANGNTTNTC NTNAA
(break)
TTTCCCTTAA AAAACCCCCA GATACAGGGA AAATTTGAGA AGAAACCGGG 
AAGAAAAAAG GGGGAATAAG GAAAAATAAA AAGGGAAAAA AAATGAGATT 
TTACCCCAAA AAATGGGAAC AAAAAATAGA CCTAAGACAA CCTAAAACCC 
CAGTTTAAAA GGGGTTTAAG GCCGCTTTAA AATAAGATTA GGGGAACGGG 
AATAACAAAA AAAAAATAGG AGGGCCGGGG CGGAAAAAAG TAAAAAAACA 
TGGAACAAAT CATGAGTAAG GGGACAATAC AGAGAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAGAGAGAA AGGCAGGGGA CGACACATGG 
CCCCTGTACG GGTGTGCTTT TGATCGCCGG ACGGGGAGGC GAGATGGATG 
ACCTCGAGGT GGAGCGAGCT CAGCCGTGAG GAAAAGAGGG CATCTTCAGC 
AAGTCCTGGT GCGGTGGCGG CTTGTCGGAA TTGGCCGGAG CAACGACCAG 
GCAACGGGGT CGAGATGCTC GGGACCATTC TGGTCCAGAT CGAGCGAGCG 
CGTCTCGAGG AGGAGCAGC

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wmc212(3h5) Plate reference : 3h5
WMC locus number : WMC212 Length of sequence : 255
Last updated : Mar 17 Nov 1998 at 10:48
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. (GT)11 158 to 179. A stuggle to get these primers - note very low Tms.
PCR Comments : 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AATATTAgcAgAATAgAcgg at 135 length 20 bases, Tm 51
Reverse primer details :
TgTATgcTcTAgcTAgATgT at 235 length 20 bases, Tm 49
Expected size of amplified fragment (Chinese Spring=) 120 bases.



Length of sequence : 255  Consensus sequence is :

..

AAAAACACAA CGCTTGAGTT CTTCTCGACA CAAAGTTCAC TCGCTTGAGT 
TGTGCAGGAC ACTTGATTCA AACAATGACG TGCACTTGCA TTGAGCATGT 
AGTGCGGAAT TGTTATCAGA ATAGTTATTC TGCTAATATT AGCAGAATAG 
ACGGGCTGTG TGTGTGTGTG TGTGTGTGTT GTGTGTGTGT TAAACACATT 
GTATGTAGTA TATCATACAT GGTTTAGATT CTGRACATCT AGCTAGAGCA 
TACAT

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wmc(3a6) Plate reference : 3a6
WMC locus number : WMC Length of sequence : 288
Last updated : Wed, Aug 20, 1997 at 3:32 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Matches 6c7. (GT)13 117 to 142.
PCR Comments :



Length of sequence : 288  Consensus sequence is :

..

GTCACGCTCT AACGACCCCA TGCTAGCCTA GTCAATATAT GGCACCGCTA 
GTCCGCCGCA CACATCTTAC TATTTCATTT GACATTGTTC TCATTTCTGT 
CCTTCCTCGT GTGTGCGTGT GTGTGTGTGT GTGTGTGTGT GTGCCCAATT 
ATGTGCATGC ACATTCTGAG TTGCCTATCG CAGTTCGCTA CGCAACTCGC 
CTCTTTTAGT TCTTCATTCA CACGCACCCT ATTACAAGGG AGTCGCAGGA 
TCAAACAGCC CTAAACAAGT CTCCAACTAA CCTACTAC

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wmc(3b6) Plate reference : 3b6
WMC locus number : WMC Length of sequence : 569
Last updated : Mer 14 juin 2000 at 10:35
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Matches 6a3.(GA)12 129 to 152, (GA)8 314 to 329, (GA)8 360 to 375, (GA)11 427 to 448, (GA)11 534 to 555, Bases 5 to 57 show homology to TW1c12.1 bases 12 to 64 (53 in longest run, maxScore = 74).
PCR Comments :



Length of sequence : 569  Consensus sequence is :

..

AGTAGAGAGG GACCAACAAG GTTTGTGTGT TGGATGCGTG CGACAGAACT 
GAAGATTGTC XXAGAGAGAG AGAGAGGGAC CAACAAGGTT TGTGTGTTGG 
ATgCGTGCGA CAAAACTGAA GATTGTCTGA GAGAGAGAGA GAGAGAGAGA 
gATKGAGGgA GGGGAGAGGG AGAAACCAGG TTTGTGTGTT AGATGCCTGC 
GATAGAACTG GAGATTWTCM GAGAGAGAGA AAGAGAGAGA GATGGAGAGA 
AAGGGAGAGG GACCCMCMAG AATTTTTTGT TGGATGCATG CSACMGAATT 
GAAGATTGTT GGTGAGAGAG AGAGAGAGAG GGAGAGAGGG AGAGAGAGGG 
AGAGAGAGGG AGAGAGAGAG AGAGATGGAG AGAAAGAGAG AAGGAGAGAG 
GAGAGAGTGA GAGAGAGAGA GAGGGMGAGA GAGAGAGAGA GAGAGAGATG 
GAGAGAAAGA GATGGAGAGA AAGGGAGAGG GACCAACAAG AATTTTGTGT 
TGGATGCATG CGACAGAATT GAAGATTCTC GGTGAGAGAG AGAGAGAGAG 
AGAGAGGGAG AGAGAGAGG

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wmc213(3c6) Plate reference : 3c6
WMC locus number : WMC213 Length of sequence : 195
Last updated : Mar 17 Nov 1998 at 10:48
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. (CA)13 36 to 61, (CA)10 88 to 107. Bases 98-195 (ie 3' side of microsatellite) match (weakly) part of 2h12. Reverse primer here is within well matched region.
PCR Comments : 51C anneal, product.
Forward primer details :
ATTTTcTcAAAcAcAccccg at 8 length 20 bases, Tm 62
Reverse primer details :
TAgcAgATgTTgAcAATggA at 172 length 20 bases, Tm 57
Expected size of amplified fragment (Chinese Spring=) 184 bases.



Length of sequence : 195  Consensus sequence is :

..

CCGCGTCATT TTCTCAAACA CACCCCGCCC CCCTCCACAC ACACACACAC 
ACACACACAC AGACACTAAA ACCTCCTCGG GCGTGTGCAC ACACACACAC 
ACACACACTC ACACACCCTC CCTCTCTCAA AACCCTATCA AGGTCCCCGC 
CGTCACCGCT GCCGCAAGGC CTCCATTGTC AACATCTGCT AGTTT

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wmc(3d6) Plate reference : 3d6
WMC locus number : WMC Length of sequence : 146
Last updated : Mon, Dec 8, 1997 at 4:24 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Bases 1-82 (ie. 5' side of microsatellite) match part of 6G2. Complex CA TA microsatellite. No room for primers microsatellite.
PCR Comments :



Length of sequence : 146  Consensus sequence is :

..

CCGGCGGATC TTTTTTTCAT ACACACACGC ATACACACAC ATACACACAC 
ACTACACACA CACATACACG CACACACGCA TACACACATA CACACACACA 
CACTACACAA ACACATACAT GCACACACGC ATACACACAT ACACAC

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wmc(3e6) Plate reference : 3e6
WMC locus number : WMC Length of sequence : 178
Last updated : Wed, Aug 20, 1997 at 3:36 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Matches 2G7. (GA)37 27 to 100.
PCR Comments :



Length of sequence : 178  Consensus sequence is :

..

GGAACGCTGC CGTGAACTCA AAATCAGAGA GAGAGAGAGA GAGAGAGAGA 
GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
TGGGGGGACT CACGGGGTTC TTGGAGCGCC CAATTAACTC TAGAACGACG 
AAATAGTAGA TGAATGCGCC TAGTCTCT

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wmc(3f6) Plate reference : 3f6
WMC locus number : WMC Length of sequence : 324
Last updated : Mer 21 Juil 1999 at 10:13
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Matches 8a12, 1C4, 11c7. (CA)13 198 to 223. Bases 213 to 246 show homology to 21f3 bases 60 to 93 (complement) (34 in longest run, maxScore = 60), duplicate, 3f6 has priority. Bases 197 to 237 show homology to 24e3.1 bases 181 to 221 (41 in longest run, maxScore = 70). Bases 128 to 159 show homology to 26h7 bases 101 to 132 (complement) (32 in longest run, maxScore = 41). Duplicate with 26h7, 3f6 has priority.
PCR Comments :



Length of sequence : 324  Consensus sequence is :

..

AGCATACAGT TCACTCGGAT GAACTGTTTG TGATTAGGCA ACACAAAAGA 
AACGGTCAGC CAGATGAAGG TGTGTGCGAT ATACGGCATG CGGTTCACTC 
GGACGAACTG TTTGCGTTGA CACAAGAGAA CAGAAACGGT TCAACTTAAC 
AAGACGTGTG TGTTGTGCGA TGGGATTGCA TACAAAACAA CAACATACAC 
ACACACACAC ACACACACAC ACACTTATAA GGACATGCTT GCATAACCAA 
ATTAAACATC CACTTACATA GGTGGCTACT ACAACCATGG CATTTGCACA 
CACCAAAGTA AACTATTACA TGCA

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wmc(3g6) Plate reference : 3g6
WMC locus number : WMC Length of sequence : 372
Last updated : Wed, Aug 20, 1997 at 3:38 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Member of 1g1 family. (CA)11 75 to 96, (CA)8 147 to 162.
PCR Comments :



Length of sequence : 372  Consensus sequence is :

..

CACACACGSA CGCACGCACC CCCCACACAC ACACTAAxxG gACTCCATCT 
CTCTCTCTCA CACACACACA CRRACACACA CACACACACA CACACASGCA 
CGCACGCACC CCCTCCCACA CACASTAAGA CTCCATCTCT CTCTCTCACA 
CACACACACA CAAACTAAGA CTCCATCTCT CTCTCTCCTC TCTCTCACAC 
ACCCACACAC ACACTAAGAC TCCATCTCAC ACACACATGC TCTAGGCGGA 
GCATTTGTTC CTACCACCCT TCATCCAACC TTACCCGTAT GATAAACAAT 
CACCcTTTAT TTACTTGxCA TAACATGGAa CAAATTCCAC tTTTACTTTA 
GTAGTCAGCA AACTTATCAT CT

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wmc(3h6) Plate reference : 3h6
WMC locus number : WMC Length of sequence : 216
Last updated : Wed, Aug 20, 1997 at 3:39 pm
Owner : Jack, Plant Breeding International Date at Agrogene : 19.3.97 Public Domain : Yes
Comments : No trimming done to insert. Member of 1g1 family. (GA)9 95 to 112.
PCR Comments :



Length of sequence : 216  Consensus sequence is :

..

GGGTAAGGTT GGATGAAGGG TGGTAGGAAC AAATGCTCCG CCTAGAGCAT 
GTGTGTGTGA GATGGAGTCT TAGTGTGTGT GTGGGTGTGT GTGTGAGAGA 
GAGAGAGAGA GAGGAGAGAG AGATGGAGTC TTAGTTTGTG TGTGTGTGTG 
AGAGAGAGAG ATGGAGTCTT AGTGTGTGTG TGTGGGGGGG GGGGTGCGTG 
CACGCATGTG TGTGTG

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wmc(11A7) Plate reference : 11A7
WMC locus number : WMC Length of sequence : 194
Last updated : Thu, Aug 21, 1997 at 9:55 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.1. Trimmed to insert at 3' end, trimmed off vector at 5' end (corrupted linker). No substantial microsatellite. Matches 9E3.
PCR Comments :



Length of sequence : 194  Consensus sequence is :

..

TCTACGGTGG AGAGGTGCAG GGACTTACAG GTGCGGAGTT ACGGCGGGGG 
TTCGTCGCGT TTCTCTGGTC ACGTCAGGTT GATGCAGCGG CCTGAGTTGG 
TGAAGATGAG GAACCCGCGG CGGCGGCGGC GGAGCTCGGG CGGCAGCATA 
GTGGCGAGAG GAGGAAGAAG ATGGAGGCGA CGAGTGACTG GAGC

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wmc(11b7) Plate reference : 11b7
WMC locus number : WMC Length of sequence : 441
Last updated : Mon, Apr 7, 1997 at 10:46 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.2. Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 441  Consensus sequence is :

..

GTACAAATTG GGAAGGATAT GGTTTTGTGG GTAGACTGGA CGCACTAAAA 
CACGGCACCC GAACGCGACC GGACGAACCG GACACGGAGC GTGAATGGTC 
AGTTCCTTCC TTCGTCCCCA AGGCCCAAAC CGCGGGGTGC GGCTCGGCCC 
CTTCCTCCCC GTCCTTTTCT CGCGCACTCG GCACTCGCAT AGTCATCGCA 
TCTTGGCTGC TCACCCCCCC GGCACCTGCA GAATCCCCAT CCGCGTTCCA 
CTTCCACCGA GGCGGAGGAT GGCCTCCGAC GACAGCAGCT CGAGCCACCC 
CACGGAGAAG AAGGAGGAGG CCTCCGCCGC CGCCGCCGCC GGTGGAGCTG 
GCTTCGACGA TCTGGAGGAC CCGCGGTTCC AGTGCTGCGT ATGCCTGTGA 
GTTTACACGC CACCTTTCTC CTCCTCTCCG TTGGCGAGGC C

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wmc(11c7) Plate reference : 11c7
WMC locus number : WMC Length of sequence : 225
Last updated : Thu, Aug 21, 1997 at 9:54 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.3. Trimmed to insert at 5' end and to Rsa1 at 3' end. First 71 bases from 3' end match complement of 8a12 and 8b12 first 71 bases. (GT)13 1 to 26. No room for primers. Matches 3F6.
PCR Comments :



Length of sequence : 225  Consensus sequence is :

..

GTGTGTGTGT GTGTGTGTGT GTGTGTATGT TGTTGTTCTG TATGCAATCC 
CATCGCACAA CACACACATC TTGTTAAGTT GACCGTTTCT GCTCTCTTGT 
GTCAACGCAA ACAGTTCATC CGAGTGAACC GCATGCCCTA TATCGCACAC 
ACCTTCATCT GGCTGACCGT TTCTTTTGTG TTGCCTAATC ACAAACAGTT 
CATCCAAGTG AACCATATGT TGTAC

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wmc214(11d7) Plate reference : 11d7
WMC locus number : WMC214 Length of sequence : 323
Last updated : Lun 5 juil 1999 at 17:23
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.4. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)9 233 to 250, (GT)10 261 to 280. Bases 77-167 are related to the 1g1, 6b11 family. Bases 233 to 269 show homology to 25b2 bases 84 to 120 (37 in longest run, maxScore = 27). Bases 106 to 143 show homology to 26a11 bases 39 to 76 (38 in longest run, maxScore = 55).
PCR Comments : 61C anneal, product; 51C anneal, smear;
Forward primer details :
AAgggTggTAggAAcAAATgcT at 100 length 22 bases, Tm 63
Reverse primer details :
TAAATTAcATccgccATAgAgg at 289 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 211 bases.



Length of sequence : 323  Consensus sequence is :

..

GTACAGATGA TAAGTTTGCT GACTACTAAA GTAAAGTGGA ATTTGTCCAT 
GCTATGCAAG TAAATAAAGG TGATTGTATC ATACGGGTAA GGTTGGATGA 
AGGGTGGTAG GAACAAATGC TCCGCCTAGA GCATGTGTGT GTGAGATGGA 
GTCTTAGTGT GTGTGTGTGA GAGAGAGAGG AGAGAGAGAG AGAGGAGAGA 
GAGATGGAGT CTTAGTTTGT GTGTGTGTGT GTGAGAGAGA GAGAGAGAGA 
TGGAGTCTTA GTGTGTGTGT GTGTGTGTGT CGCGGGGTCC TCTATGGCGG 
ATGTAATTTA AGTGTGCATG GCT

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wmc215(11e7) Plate reference : 11e7
WMC locus number : WMC215 Length of sequence : 246
Last updated : Fri, Jun 13, 1997 at 20:09
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
PIC Value : 0.79
Comments : AGR205.5. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)29 66 to 12.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cATgcATggTTgcAAgcAAAAg at 16 length 22 bases, Tm 67
Reverse primer details :
cATcccggTgcAAcATcTgAAA at 201 length 22 bases, Tm 69
Expected size of amplified fragment (Chinese Spring=) 207 bases.



Length of sequence : 246  Consensus sequence is :

..

GTACTATCCG TCTAACATGC ATGGTTGCAA GCAAAAGGAA ACTACAGTGA 
TGTAATGTTA ACGATGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTCAGCCTG TGGGTCATTG ACCAGTTGAT 
TCATCTGCGT GAGTTTTTAC GGCAAGAAAT GCAGATTTTA TCGCGGGTAG 
TTTCAGATGT TGCACCGGGA TGGCAAGGCA GGCACGTTAG TCCACG

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wmc(11f7) Plate reference : 11f7
WMC locus number : WMC Length of sequence : 272
Last updated : Fri, Jun 13, 1997 at 20:11
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.6. Trimmed to insert at 5' end and to Rsa1 at 3' end. matches 11g6. Group of small microsatellites (GT)6(GC)4(TG)5A(TG)5.
PCR Comments :



Length of sequence : 272  Consensus sequence is :

..

GGATGGGGAT GAGGATCTGT GGGAAAGGGG GCATTTGGCA GGCGTCATCT 
AAGAAACTCA AGGCAGCCTG GGTATGGAGA TCAGTGGGTA AGCTGCACGG 
GCAAACCGGT AGATGTTGAC AATGGAGGCC TTGCGGCAGC ATGGACATTT 
CTAGGGTTTC GAGTGAGGGA GGGGGTGTGT GTGTGTGCGC GCGCCCGAGG 
AGGTTTTGTT GTGTGTGTGT ATGTGTGTGT GGAGGGTGGG AGCGGGGGTG 
TTTGAGGAAA TGACGGGGGT AC

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wmc(11g7) Plate reference : 11g7
WMC locus number : WMC Length of sequence : 373
Last updated : Mon, Apr 7, 1997 at 10:49 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.7. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 373  Consensus sequence is :

..

GTATCAGTGT CTGATTGTTA AAACAAAGAG TGGCTCTTGG CCTCTTGCAT 
GCATGCTAGA CTAACGGCAT CGAGCCAGTT TTTTGTAAAA AAAATAAACC 
GGCATCGAGC CCTTACTTTT TTTTTTGAAA AAACTGCATC GAGCTGAATC 
TGCTGAATGC TCCTTGCCCA ACCGCATCAG CAAGCTGAGT GCTCACCGAG 
AAAACCCAGG CAGTCAAATT CCCAGTCGAA TAGGAGCAGA GCAGCACAGA 
TCTGCCGCCT CGCCCATGCC AACCCCATGC CACTCTCGTC ACCACTCCGC 
CTCATCCTCT CCTCCCGCCG CCGGCCCCTC GCCACGCACC ACCGCTGCCT 
GTCCTCCTCC TCCGCCGCCG CCG

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wmc216(11h7) Plate reference : 11h7
WMC locus number : WMC216 Length of sequence : 241
Last updated : Thu, Jun 11, 1998 at 11:30 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.8. Trimed to insert at 5' end and to Rsa1 at 3' end. (GT)22 42 to 85.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AcgTATccAgAcAcTgTggTAA at 13 length 22 bases, Tm 59
Reverse primer details :
TAATggTggATccATgATAgcc at 114 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 123 bases.



Length of sequence : 241  Consensus sequence is :

..

CCACATACAC GCACGTATCC AGACACTGTG GTAAGCTTTT TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTAGGGA CAGAAGATCG 
CCGGAGATGG AGCGGCTATC ATGGATCCAC CATTATTGAA GGCGTAACAT 
CAAAGGATTG CATTCATATT TAAAGTCGTG GCAGTGCAAG AGAGAGCAAG 
CCAGCCAAGG CGATTGGATC TTGCACCTGC CAACAAGGTA C

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wmc(11a8) Plate reference : 11a8
WMC locus number : WMC Length of sequence : 417
Last updated : Lun 9 oct 2000 at 14:43
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.9. Trimmed to insert at 5' end (NB linker sequence chewed back). Unable to locate 3' end.(GT)9 6 to 23, (GT)12 126 to 149. Complex internal repeats of minisatellite - like structure, too difficult to design primers. Bases 348 to 397 show homology to 22c7 bases 149 to 198 (50 in longest run, maxScore = 42). Bases 350 to 376 show homology to TW2f02.2 bases 443 to 469 (27 in longest run, maxScore = 22).
PCR Comments :



Length of sequence : 417  Consensus sequence is :

..

TAAGGGTGTG TGTGTGTGTG TGTGGATATT GCCCGCAGGC ATGGTGACCC 
AGGCTTTGGT GGCAAAAGGC CATGTAGCCT CTGGAGGCAC GGGAGGGTGG 
TCTCCAGAGT CGCGCGTAAG GATATGTGTG TGTGTGTGTG TGTGTGTGTG 
GATATTGCCC GGAGGCATGG TGACCCAGGC TTTGGTGGCA CAAGGCCATG 
TCGCCTCTGG AGGCACGGGA GGGTGGTCTC CAGAGTCACG CATAAGGGTA 
TGTGTGTGTG TGGATATTGC CCGGAGGCAT GGTGACCCAG GGTTTGGTGG 
CACAAGGCCA TGTCGCCTCT AGAGGCACTG GAGGATGGTT TCCAGAGTCA 
CGCGCCTCGA GGGATCCTCT AGAGTCGACC TGCAGGCATG CAAGCTTGGC 
GTAATCATGG TCATAGC

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wmc(11b8) Plate reference : 11b8
WMC locus number : WMC Length of sequence : 245
Last updated : Fri, Jun 13, 1997 at 5:35 pm
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.10. Trimed to insert at 5' end and to Rsa1 at 3' end. (GT)12 207 to 230. No room for primers.
PCR Comments :



Length of sequence : 245  Consensus sequence is :

..

GTGTGCACAA TAAAGCCAAT TCAGATTCAT ACAAAATGAA AAGGCTTTTA 
TGCAATTCAG AGCGTAAAGT GAAAGGCTAT TGCAGTGTTC TGTATCTGCC 
ATCAGAATCA TCAATGCAGT TGTGTCGGTC GTGAGAACTA ACATTTCCCA 
TTTTCCTCGG CAGAGAAAAA CCTTGGACAT ATTTTAGTAA CCCATGCACA 
AACACTGTGT GTGTGTGTGT GTGTGTGTGT GCGTGCATGG GGTAC

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wmc(11c8) Plate reference : 11c8
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Apr 1, 1997 at 11:59 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.11. No sequence obtained by Lark.
PCR Comments :
No sequence available for this clone
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wmc(11d8) Plate reference : 11d8
WMC locus number : WMC Length of sequence : 39
Last updated : Mon, Apr 7, 1997 at 10:54 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.12. Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 39  Consensus sequence is :

..

GTACCGGTGT TCATAGAGGT TGTGGGAGAG GGACCAACA

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wmc217(11e8) Plate reference : 11e8
WMC locus number : WMC217 Length of sequence : 486
Last updated : Fri, Jun 13, 1997 at 21:26
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.13. No overlap between forward and reverse sequences. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)13 151 to 176.
PCR Comments : tested 51C anneal, no product;
Forward primer details :
ATcTcAcTgAAcATAgcTAAAg at 130 length 22 bases, Tm 52
Reverse primer details :
cAAAAgTAgcTcTcAgcTAAAg at 193 length 22 bases, Tm 55
Expected size of amplified fragment (Chinese Spring=) >85 bases.



Length of sequence : 486  Consensus sequence is :

..

GTACACATGA TGGCAAAAAT ATGAAAAACA TAATACAAAA ATGACACGGC 
AATTTTACCA AAATTTTCTC TATTTGTCAT GGCAAAATGC TGATAATATG 
TGTGTTGCAT GGCAAATTTA GGAAAAAAAA TCTCACTGAA CATAGCTAAA 
GTGTGTGTGT GTGTGTGTGT GTGTGTG
(break)
TGATTT CTGCACTCTT 
TAGCTGAGAG CTACTTTTGA TGAATAAAAT TTGCCAGTCG AGATATTACC 
TTGTTATCCA AAGCATTTTT GTAGTTTATA GTTTTATTGT TATCCTAAAT 
GGATAACCAT TTGATATCAA AATCATCAAT ACTAGTTTCT GACAAAAATC 
TTGGGTCGGT TGGTGCATTA CCGCCCGAAC TATTGAATTT CCTTTTCTTT 
TTTGAACGTT GGTAGGGGAC CTTTGAAATT CCATTGATTA GGGGAAACGG 
TTGAATTTCG TTTAATTTCC AGCAGCAACT GATTGCTACA GAC

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wmc218(11f8) Plate reference : 11f8
WMC locus number : WMC218 Length of sequence : 744
Last updated : Lun 2 Nov 1998 at 10:05
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
PIC Value : 0.3
Comments : AGR205.14. Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiples inserts (3) in this clone joined at 304-321, 502-519 by linkers. Matches 11g8. (TG)7CGTGC(GT)7 microsatellite in first insert. Primers are designed to first insert. Bases 401 to 434 show homology to 22g9 bases 109 to 142 (34 in longest run, maxScore = 52). Bases 28 to 55 show homology to 24b5 bases 153 to 180 (28 in longest run, maxScore = 38).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TcTccTgTcggcTgAAAgTgTT at 66 length 22 bases, Tm 65
Reverse primer details :
ccATggAggTTcAccTAgcAAA at 148 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 104 bases.



Length of sequence : 744  Consensus sequence is :

..

GTACAACCAA GGTGGATTAT TCTTGGACCG TTTTACGATA CATCTTGGTG 
AGATGTGAGA TTCCCTCTCC TGTCGGCTGA AAGTGTTTGT GTGTGTGTGT 
GCGTGCGTGT GTGTGTGTGT GATGGGGATG GGACTGCTAC TTCACACTTT 
GCTAGGTGAA CCTCCATGGG GGCGGGAATG CGTTCATAGC TTAGGATTCC 
CCTCCTGCCG ACACGAGGGG GGCGCAAGCA ATACCGTGCA CTTTGCGAGA 
TAGGTGCCAC ATCGAAGTTG CATTTTGTAT TACAACATCA AACCACCCAT 
TTCTAGTCCA CGCGTGGACT AACCCGTGAC ATTTCCTCAA ACACCACCTT 
GGACCCCTCC ACACACACAC CCCAAATCCT TCGCTCACCC CAAATCCCCC 
GCTCACACAC ATACACACCA CCCCTCCCTC GCTCGAAACC TAGATAGGTC 
ACCGCCGCCG CCGCCACGCC GCCTCCATCG TCAACCTCCG CTGGTCTGCC 
TTAGTCCACG CGTGGACTAA CAGGGAAGTC TTCGTCTTGG AAGGGCAGTG 
GTCCAAGCGA GCAGCAGCAC GAGGAGAGGT GGAGGCGAAG CAGGGAGGCG 
GAGCTTCTGC AAAAAGGCGG CGGCGCGGCT GAGAAGATGG ACGGCGGCGG 
GGAGCGAGAC ACTTGAGGGT GCGGGGGCAC GACGGAGCTC GGCGGGTTTG 
AGGACGACGG GTCCGGGAGG CGAAGGAGGA GCGGCCATGG AGAC

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wmc(11g8) Plate reference : 11g8
WMC locus number : WMC Length of sequence : 744
Last updated : Mon, Apr 7, 1997 at 10:59 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.15. Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiple inserts (3) in this clone joined at 304-321, 502-519 by linkers. Matches 11f8.
PCR Comments :



Length of sequence : 744  Consensus sequence is :

..

GTACAACCAA GGTGGATTAT TCTTGGACCG TTTTACGATA CATCTTGGTG 
AGATGTGAGA TTCCCTCTCC TGTCGGCTGA AAGTGTTTGT GTGTGTGTGT 
GCGTGCGTGT GTGTGTGTGT GATGGGGATG GGACTGCTAC TTCACACTTT 
GCTAGGTGAA CCTCCATGGG GGCGGGAATG CGTTCATAGC TTAGGATTCC 
CCTCCTGCCG ACACGAGGGG GGCGCAAGCA ATACCGTGCA CTTTGCGAGA 
TAGGTGCCAC ATCGAAGTTG CATTTTGTAT TACAACATCA AACCACCCAT 
TTCTAGTCCA CGCGTGGACT AACCCGTGAC ATTTCCTCAA ACACCACCTT 
GGACCCCTCC ACACACACAC CCCAAATCCT TCGCTCACCC CAAATCCCCC 
GCTCACACAC ATACACACCA CCCCTCCCTC GCTCGAAACC TAGATAGGTC 
ACCGCCGCCG CCGCCACGCC GCCTCCATCG TCAACCTCCG CTGGTCTGCC 
TTAGTCCACG CGTGGACTAA CAGGGAAGTC TTCGTCTTGG AAGGGCAGTG 
GTCCAAGCGA GCAGCAGCAC GAGGAGAGGT GGAGGCGAAG CAGGGAGGCG 
GAGCTTCTGC AAAAAGGCGG CGGCGCGGCT GAGAAGATGG ACGGCGGCGG 
GGAGCGAGAC ACTTGAGGGT GCGGGGGCAC GACGGAGCTC GGCGGGTTTG 
AGGACGACGG GTCCGGGAGG CGAAGGAGGA GCGGCCATGG AGAC

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wmc(11h8) Plate reference : 11h8
WMC locus number : WMC Length of sequence : 256
Last updated : Mon, Apr 7, 1997 at 11:00 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.16. Trimmed to insert at 5' end and to Rsa1 at 3' end. Complement of last 50 bases match 9b3. No substantial microsatellite.
PCR Comments :



Length of sequence : 256  Consensus sequence is :

..

GGTGGATATC ATTACATCAA CATTACATAA TAGATGAGGA TACATACAAA 
AGGCATACAA TGCCACACGA ATACAACATC ATGATACATA AGATCTACAT 
CCGACTACGG AGGAAACACA AATAGAAACT CAAATGACAT CCACCCTGCT 
AGCCTAGGCT GCCGACCTGG AACCTATCCC CTGATCGAAG AAGCAGAAGA 
AAAACTCAAG ACAAGCAAGC ATCGCTCTCG CGTCATGATC ATCGCATAAC 
CTGTAC

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wmc(11a9) Plate reference : 11a9
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Apr 1, 1997 at 12:00 pm
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.17. No sequence obtained from Lark.
PCR Comments :
No sequence available for this clone
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wmc219(11b9) Plate reference : 11b9
WMC locus number : WMC219 Length of sequence : 488
Last updated : Fri, Jun 13, 1997 at 22:06
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.18. Overlap of forward and reverse is hypothetical - both end within a substantial micorsatellite. The fragment sizes for the PCR from this sequence will not match the expected size. Trimmed to Rsa1 at both ends. (CA)57 316 to 429.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TgcTAgTTTgTcATccgggcgA at 264 length 22 bases, Tm 71
Reverse primer details :
cAATcccgTTcTAcAAgTTccA at 446 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) ~204 bases.



Length of sequence : 488  Consensus sequence is :

..

GTACGTGTAA ACAAAGAGCT CTTCGAGAGT GGTGAGCGAA CTTCTCAATC 
AGGCACAGAA CACGTCTGGC ACCCATTTTT TTGCTGGCAA CGTTGTATGT 
ATGGACGTTT ATTCAAAAAT TCTAATTAAG AGTAGGTTGC AGCAGAGAGA 
CCAAGCTTGA CATGCAAACC CAGCATATAT ATATAAGCTC CAAGCTTGCT 
ATAGTATTGT CTTTGCTATA GCATACTGCT CAAGATTTGG CAATCCTACC 
AGCAAGAAAA TGCTGCTAGT TTGTCATCCG GGCGAATGGA CGATGGTATG 
CCAAGACGAA ATTTCCACAC ACACACACAC ACACACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAA ATCGAGAGCT TGTAATGGAA 
CTTGTAGAAC GGGATTGTAA CATTTAAACT TTGAGTAC

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wmc(11c9) Plate reference : 11c9
WMC locus number : WMC Length of sequence : 287
Last updated : Mon, Apr 7, 1997 at 11:02 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.19. Trimmed to Rsa1 at 5' end and to insert at 3' end. Matches 9c2. No substantial microsatellite.
PCR Comments :



Length of sequence : 287  Consensus sequence is :

..

GTACATAGGT TCTGCTGAGG GCATAGCTCA ACAAGCCCAA ACAAAAAATA 
AAAAAGGCAG CAAATGCCGG GCGAAGGAGA CGTCTAGTCT GGCTCCGGCG 
GAGGCGGCGG GGGAGGCGGC GACAGGCGGA CGACCATCGA TCGGAGGTCG 
GCGATGAAGG CTGAGATGGC GTCACGGTCC AGGGGACGGC TAAGTGGCCG 
CCAAAGCTGC AAAAAACTCG AGAGTTTAAA GAGCGCGTCA GTAGATCGCC 
GAAGAGGAGT GCGCTGAATC ACGAGCTTAT TGCGAAT

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wmc220(11d9) Plate reference : 11d9
WMC locus number : WMC220 Length of sequence : 199
Last updated : Thu, Aug 21, 1997 at 10:05 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.20. Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)10 52 to 71. Bases 98-198 (ie 3' side of microsatellite) match part of 2h12.
PCR Comments : 61C anneal, product; 51C anneal, product;
Forward primer details :
TAcccgcgTcATTTccAcAA at 2 length 20 bases, Tm 66
Reverse primer details :
TggTTTcgAgcgAgggAgAg at 108 length 20 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 126 bases.



Length of sequence : 199  Consensus sequence is :

..

GTACCCGCGT CATTTCCACA AACACCCCCC CTCCACACAC ACGCACACAA 
ACACACACAC ACACACACAC ACGAAAACCT CCTCGGGCAC ACGCGCCCAC 
ACACACACTC TCCCTCGCTC GAAACCATAG CAAGGTCCCC GCCACCGCCG 
CCGCCGCGAG ACCTCCATTG TCAACATCTG CCGGTTTGCC CGTGCAGCG

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wmc(11e9) Plate reference : 11e9
WMC locus number : WMC Length of sequence : 293
Last updated : Mon, Apr 7, 1997 at 11:05 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.21. Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 293  Consensus sequence is :

..

GTACACTCTG CCGTCAGCTC GCGTCGACCT CCTGTGGTAT GCGCCGCGCC 
GTCTCCTTGG TGCGGGAATG CCACCATCCG CACCTGTCTT CGTCATGCTG 
TCAGGGTTCT TCGCCTACTT CGAGCACCAC CGCCGCGCTA CCTAGCCATT 
GCCGCCATTA GGCCGCCGCC GCCGCTCTTC TTCGGGCCGC TGCCACCGCT 
ACCCTCGTAG CCGTCGCCAC CCGTCCACCT CCGTCGTCTT CGCCCAGCAC 
CAGCTCGTCA CCAGCATCTC CGTCATCTTT CCCGACCACT TCG

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wmc(11f9) Plate reference : 11f9
WMC locus number : WMC Length of sequence : 427
Last updated : Lun 28 DŽc 1998 at 14:26
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.22. Trimmed to insert at 5' end and to Rsa1 at 3' end. Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 5H10 9H3, 4b7, 4g9, 4c12, 11f9. (CA)8 134 to 149, (CT)8 243 to 258. Bases 315 to 345 show homology to 21a11 bases 1 to 31 (complement) (31 in longest run, maxScore = 41). Duplicate with 21a11, 11f9 has priority.
PCR Comments :



Length of sequence : 427  Consensus sequence is :

..

CATCACCTCT CTATGATGAA AGCCATGCAC ACTTAAATTA CATCCGCCAC 
AAAGGACCCC GCGACACACA CGCACGCACC CCCCCAACAC ACACACTAAG 
ACTCCATCTC TCTCTCTCAC ACACACACAC AAACACACAC ACACACACAT 
GCAAGCACGC ACGCCCCCCC CCACACACAT AGACTCCATC TCCCTCCCTC 
TCTCTCACAC ACACACAAAC TAACACTCCA TCTCTCTCTC TCCTCTCTCT 
CTCTCTCTCA CACACACACT AAGACTCCAT CTCACACACA CATGCTCTAG 
GCGGAGCATT TGTTCCTACC ACCCTTCATC CAACCTTACC CGTATGATAA 
AAAACCACCT TTATTTACTT GCATAACATG GACAAATTCC ACTTTACTTT 
AGTAGTCAGC AAACTTATCA TCTGTAC

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wmc221(11g9) Plate reference : 11g9
WMC locus number : WMC221 Length of sequence : 280
Last updated : Tue, Jun 17, 1997 at 8:46 am
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
PIC Value : 0.48
Comments : AGR205.23. Trimmed to Rsa1 at both ends. (CA)16 176 to 207, (CT)9 158 to 175.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
AcgATAATgcAgcggggAAT at 40 length 20 bases, Tm 65
Reverse primer details :
gcTgggATcAAgggATcAAT at 229 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 209 bases.



Length of sequence : 280  Consensus sequence is :

..

GTACTGGCAG ATTGGAGGTG CTTGAGGAGG AGGATTCTGA CGATAATGCA 
GCGGGGAATG AGAGCCAACG CACCACCTCC AATGCGGATT CTCTTCTTCG 
TGATCTTCAG GCTCAAGGGA GAAAGCTTGG TAACAACCCG AACCCCTCCT 
GCATCTCCTC TCTCTCTCTC TCTCTCACAC ACACACACAC ACACACACAC 
ACACACACTC TCCTCCGTAT CTTACTTGAT TGATCCCTTG ATCCCAGCTC 
TGGAGAGATT CAGGAGAATG TGCAGAGTAC

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wmc(11h9) Plate reference : 11h9
WMC locus number : WMC Length of sequence :
Last updated : Wed, Apr 9, 1997 at 2:32 pm
Owner : Benoist Date at Agrogene : 24.3.97 Public Domain : Yes
Comments : AGR205.24. Cannot find linker sequences - sequence was only vector - no insert.
PCR Comments :
No sequence available for this clone
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