Wheat microsatellite WMC 25/06/01

wmc242(5a4) Plate reference : 5a4
WMC locus number : WMC242 Length of sequence : 329
Last updated : Lun 2 Nov 1998 at 10:05
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)10 110 to 129. Bases 189 to 226 show homology to 17d11 bases 14 to 51 (complement) (38 in longest run, maxScore = 44). Bases 178 to 226 show homology to 10d10 bases 233 to 281 (complement) (49 in longest run, maxScore = 118). Bases 30 to 99 show homology to 24f4 bases 66 to 135 (complement) (70 in longest run, maxScore = 91).
PCR Comments : 51C anneal, product;
Forward primer details :
ggTATcccTgcTAAgTTTTTcAATg at 19 length 25 bases, Tm 63
Reverse primer details :
cTAATATTTAggAAcAgAAggAgTA at 217 length 25 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 223 bases.



Length of sequence : 329  Consensus sequence is :

..

GTGGATTTCA CATTTCATGG TATCCCTGCT AAGTTTTTCA ATGCAAATTC 
AAATTTAAAA GATGAACAAT ATGGAGGTGA GAAAACTTTG TATGTATCAA 
GCATATGACC ACACACACAC ACACACACAG ACACCCACAC ACGAACACAA 
CAACAATCCA ATAAGTATAG TGCATATATT TTTCCAAACC ATGCATGTAT 
GACACGAATT CAAAAATACT CCTTCTGTTC CTAAATATTA GTCTTTTAGA 
GATTTCAACA AGCGACTACA TACGGAATAA AATGAGTGAA TCTACACTCT 
AAAATATGTC TATATACAGC CGTATGTAC

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wmc(5b4) Plate reference : 5b4
WMC locus number : WMC Length of sequence : 110
Last updated : Thu, Aug 21, 1997 at 2:21 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end. small insert - 3' linker appears corrupted. Matches 1A3, 5d4, 1a3, 5e1, 4h1o(1). No substantial microsatellite.
PCR Comments :



Length of sequence : 110  Consensus sequence is :

..

GTACACCGAC CGATCTCCCG CTATGCATAT AGCTAGCTAG GTCCTTATAA 
CTTATTTTTC CCCACGCGCC TCGAGGGATC CTCTAGAGTC GACCTGCAGG 
CATGCAAGCT

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wmc(5c4) Plate reference : 5c4
WMC locus number : WMC Length of sequence : 0
Last updated : Thu, Aug 21, 1997 at 2:25 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : No insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 0  Consensus sequence is :

..

GAGGCGCGCC TCGAGGGATC CTCTAGAGTC GACCTGCAGG CATGCAAGCT 
TGG

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wmc(5d4) Plate reference : 5d4
WMC locus number : WMC Length of sequence : 106
Last updated : Thu, Aug 21, 1997 at 2:28 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end. Matches 5e1, 5b4, 1a3, 4H10(1). No substantial microsatellite.
PCR Comments :



Length of sequence : 106  Consensus sequence is :

..

CGAGGCGCGT GGGAAAAACG ACTTATAAGG ACCTAGCTAG CTATATGTAT 
AGCGGGAGAT CGGTCGGTGTAC

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wmc243(5e4) Plate reference : 5e4
WMC locus number : WMC243 Length of sequence : 229
Last updated : Thu, Aug 28, 1997 at 9:03 am
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)15 39 to 68. Small common sequence with 11D6 avoided.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cgTcATTTccTcAAAcAcAccT at 8 length 22 bases, Tm 63
Reverse primer details :
AccggcAgATgTTgAcAATAgT at 163 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 177 bases.



Length of sequence : 229  Consensus sequence is :

..

GTACCCGCGT CATTTCCTCA AACACACCTC CCTACCTCCA CACACACACA 
CACACACACA CACACACAAA CACCAAAACC TCCTCGGGCG CGTGCACACA 
CGCACACACA CACCCTCCCT TGCTCAAAAC CCTAGAAAGG TCCCCACCAC 
CGCCGCGAGA CCACTATTGT CAACATCTGC CGGTTTTCCG TGCAGCTTAC 
CCACCGACCT CCATACCAAG ACGCCTGCC

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wmc(5f4) Plate reference : 5f4
WMC locus number : WMC Length of sequence : 230
Last updated : Mar 30 mai 2000 at 9:54
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite. Very GC rich. Bases 138 to 151 show homology to TW4g10.2 bases 71 to 84 (complement) (14 in longest run, maxScore = 26).
PCR Comments :



Length of sequence : 230  Consensus sequence is :

..

CGCCGGGCGC GGCGGTGGAG GAGGAGGACG ACGACGACAT GGCGGCGGCG 
GCGATGAAGC GGAGGGGCGA GGAGCAGGAG CTGGGGCGGT GGCGGAGGCG 
GAGGAGACCT CCACGACGAG GTAAAAATCT CATTGATTGC AAATGGGTGT 
TTCGCATTAA AAGAAAGTCT GACGGATCTA TAGATCGTTA CAAAGCAAGA 
CTTGTTGCAA AGGGCTTCAA ACAACGGTAC

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wmc(5g4) Plate reference : 5g4
WMC locus number : WMC Length of sequence : 641
Last updated : Thu, Aug 21, 1997 at 4:27 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at both ends, multiple inserts (3) in this clone joined at 367-382, and 478-487 by linkers and linker fragments repsectively. Last insert matches 9g5. (CA)13 386 to 411, (TTG)12 17 to 52, (TTG)9 56 to 82, (TTG)8 175 to 198, (TTG)8 202 to 225. No room for primers in any insert.
PCR Comments :



Length of sequence : 641  Consensus sequence is :

..

GTTGTTGTTG TTGCTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT 
TGTCGTTGTT GTTGTTGTTG TTGTTGTTGT TGCTGCTGTT GTTGTGCTGC 
TGCTGCTGTT GCTGCTGCTT TTGCTGTTGC TGCTGTTGCT GTTGTTGCTA 
CTGCTGTTGT TGCTACTGCT GCTGTTGTTG TTGTTGTTGT TGTTGTTGTC 
GTTGTTGTTG TTGTTGTTGT TGTTGCTGTT GTTGCTGTTG CTGTTGTTGC 
TGTTGTTGAT GTTGTTGCTG CTGTTGTTGT TGCTGTTGTT GCTGTTGTTA 
CTTTTGTTGT TGTTGTTGCT GTTGTTGTTG TTGCTATTGC TGCTGTTGCT 
GTTGCTGCTG TTGCTGTAGT CCACGCGTGG ACTAACACAC ACACACACAC 
ACACACACAC ACCGTGTGAG CTTGTGGGGC CTGTGGGCCA CCCCTTCTGT 
TGATTCTTGC GCCAAAAATC CCTAGTCCCG CGTGGACTAC ATCCTCATGC 
CTCCAAATCC CCACCACCAG GAGCTGATGG CGGTCCATGG CGCGATGGCC 
GCTGTGCGTG TTGACTCGGA GGAACACCTC GCCTCCGCCG CCGCCGCCAA 
CATGGTGCAC GCTCTGGCCC AGATGAAGTC CCCCAGCGAC T

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wmc244(5h4) Plate reference : 5h4
WMC locus number : WMC244 Length of sequence : 489
Last updated : Lun 9 oct 2000 at 14:43
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1- 159 (complement) match part of 1h6, 3d1. (CT)16 326 to 357. Forward primer avoids common sequence with 1H6. Duplicate 8G1. Reverse primer has 3 G deletions relative to 4A1. Region 150-250 contains partially duplicated sequence. Bases 326-474 match part of 4A1 (complement). Bases 326 to 374 show homology to TW2d12 bases 150 to 198 (complement) (49 in longest run, maxScore = 98).
PCR Comments : 51C anneal, product;
Forward primer details :
TcTccAcTTTTATcgTgTgTTg at 266 length 22 bases, Tm 60
Reverse primer details :
ggTTTgAcTTTTcAgAAcATcc at 390 length 22 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 146 bases.



Length of sequence : 489  Consensus sequence is :

..

CCTTGTCACT CGATCTCCCA CCCATGTAGT CCCATAACGA TATCCAGGGG 
ATCTCTCTAT GACAAACATA CACTCTCTCC TCCCCATGTC TGCATTTTCT 
CCGTACGTCT CTCTCGACCT CTCTCCCTTG TTTGTCAGAC CCCTCTTGGC 
CACGTGCTAG GGGTCTTCCT CTCTCGGTTC CAAACAACCA CACACTATCT 
CCTCACCTTG CCTCCATTGT CGAAGATGCA TGTGTGATAT GTTTGCATAA 
GACACGCACG ATTTTTCTCC ACTTTTATCG TGTGTTGTCC ATGTCTCTTT 
CACACACGCA CACACACTTT TTTCACTCTC TCTCTCTCTC TCTCTCTCTC 
TCTCTCTCGT CAGGGACTCT GTCACGTCCA TAAGCATATG GATGTTCTGA 
AAAGTCAAAC CTCAAAAAAG TTTGACTAGG TATATGTGGA GAAAAACATT 
TACATCTGGA ATGATCATTA GATTCATCAC AACATGTAC

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wmc(5a5) Plate reference : 5a5
WMC locus number : WMC Length of sequence : 49
Last updated : Mon, Apr 21, 1997 at 9:55 am
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : No insert. No substantial microsatellite.
PCR Comments :



Length of sequence : 49  Consensus sequence is :

..

GCGCCTCGAG GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGC

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wmc245(5b5) Plate reference : 5b5
WMC locus number : WMC245 Length of sequence : 676
Last updated : Mar 22 DŽc 1998 at 17:51
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
PIC Value : 0.48
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiple inserts in this clone (2) separated at 314-331 by linkers. First insert is duplicate of 7a11. (CA)10 261 to 280, (CA)10 291 to 310, (CA)13 496 to 521, (CT)8 480 to 495. Bases 136 to 167 show homology to 2g3 bases 76 to 107 (32 in longest run, maxScore = 79). Bases 3 to 19 show homology to 25d1 bases 14 to 30 (17 in longest run, maxScore = 35). True duplicate with 2g3, 5b5 has priority.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
gcTcAgATcATccAccAAcTTc at 436 length 22 bases, Tm 63
Reverse primer details :
AgATgcTcTgggAgAgTccTTA at 564 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 150 bases.



Length of sequence : 676  Consensus sequence is :

..

GTACCCCTAG TTGCGAGTCG ATGGTCAACT TGCAACTGGG GGCCACGAAT 
AAAACACACA CACGCACGCA CGCACACATC CCTTAGTTGC GAGTCGATGG 
ACAACTTGCA ACTAGGGGTC CTCAACACAC GCACCCCCTA GTTACAAGTC 
GGTGGTTGAG ATGCAACCGG GGGCCCCTTG ACAAAAAAAC ACACACACGC 
ACGCACTTCT AGTTGCGAGT CGATTATTGA CATGCAACTA GGGACCCACG 
ACAAAACAAA CACACACACA CACACACACA CGCACACAAA CACACACACA 
CACACACACA CGTTAGTCCA CGCGTGGACT AACCACCCAA GGATATACAT 
ATGTAGATAG ATCAAGACCC GGCCTTACAT ATTCCCCAAA CCTCCTCTGA 
AAACTCCACT TGCATGCTAG CAGAGGCTGT CAGGAGCTCA GATCATCCAC 
CAACTTCCTT TGGCCCCTCT CCCCTCCATC TCTCTCTCTC TCTCTCACAC 
ACACACACAC ACACACACAC ACTCCTTTCC TGCTCCAGAT TTGTGGCATT 
GACCACTCAT CAGTAAGGAC TCTCCCAGAG CATCTGCCCT CCCTTGGGCA 
TTTTGCCATT GGCAGCTTCG GCTTGGGCTT TTCTGCGAGC GAGGTGTGGG 
TGGTGGCGCT GCCATCCCCT TTGTGC

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wmc(5c5) Plate reference : 5c5
WMC locus number : WMC Length of sequence : 381
Last updated : Lun 30 oct 2000 at 16:59
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, cannot locate 5' end. No substantial microsatellite. Bases 354 to 364 show homology to TW6c04.1 bases 312 to 322 (complement) (11 in longest run, maxScore = 23).
PCR Comments :



Length of sequence : 381  Consensus sequence is :

..

CGAGGCGCGT GGTTTGGGGC TACGGGTTGC TGTGGTTTGC ATGCATCTGA 
TGGAGCAAGC GAGGGGAGCT GGGATGAGCT GTGGCGTCGG CGGTGGCGAG 
CGAGGTGGTG GCGAGCGAGG CGGCGGCCGG AGTTTGGGCA TCAGCAAAAA 
CGATGCTGCA GCGCACGGCA GGAGCAACGG GTGAGCGTTC TAGGCTCCTG 
ACGGCGTTCT GGGCATGTTG CAGTGCTCGG TTGTCGTCAT AGCTATCTCT 
GGCCGCGGCG GCGATGAGGC CTAACGGTGG CGGCTTCGGA TTTCATGGGG 
GCGGCGGCTA CAGGATGAGC AGTAAGCTGG TGCAGCTGCA AGCAGAGCGT 
CGTGGCGGGA TCTACGTGTG AAGCGGAGTA C

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wmc246(5d5.1) Plate reference : 5d5.1
WMC locus number : WMC246 Length of sequence : 466
Last updated : Mar 10 Nov 1998 at 15:07
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Multiple inserts in this clone (3) joined at 467-484 and 760-777 by linkers. Sequence given here is first insert only (thers have separate records). Bases 148-249 match part of 4E2 (complement) - near (NA)n stretch. (CA)15 38 to 67. Primers shown are for first insert, avoids small homology to 4E2. Bases 46 to 67 show homology to 23A1 bases 88 to 109 (complement) (22 in longest run, maxScore = 21). Bases 37 to 67 show homology to 21f4 bases 176 to 206 (31 in longest run, maxScore = 50), duplicate, 5d5.1 has priority.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TAccAgAgTgTATggTTgcAAAgT at 2 length 24 bases, Tm 62
Reverse primer details :
AAcAcATgATATAgcAAccAAggA at 274 length 24 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 296 bases.



Length of sequence : 466  Consensus sequence is :

..

GTACCAGAGT GTATGGTTGC AAAGTGTCAT TAATGAACAC ACACACACAC 
ACACACACAC ACACACATAT ATATATATAT ACATGTATAA CAAGAATGCA 
ATAATAGCTA TAATGTCATG CATTGTTTAT CGAAAAATTG CGTTAAAGCA 
GAGTGATACA GATAGTGCGA TAAGCAAAAG AGTAGGACTA TGTCCCTTCC 
AAGGGCGAGC TGAGGAATGA TATAAAATAT GTAATCAACC TGGGAATTCC 
GTTGTATAAC GTAGCTGTCT GCCTCCTTGG TTGCTATATC ATGTGTTCGG 
CAATAGAGCT GCCGGACCGT GTTTCCAGAG ATTAAAGTCC TGAAAGAAAA 
AAGAAAAATA ACCAAACGGG AAGCCCCTAG TGTGGTTTAG GCCGCATTTT 
GGGGCGTGCC GCAGTTGTGC CCCCCCCCCC GCCTGTGCCC ATGGTATTTT 
TAATGCGTAA TTATGT

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wmc247(5d5.2) Plate reference : 5d5.2
WMC locus number : WMC247 Length of sequence : 275
Last updated : Thu, Aug 28, 1997 at 11:01 am
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Derived from full sequence - this is the second insert only, without linkers. Primers shown are for this insert insert. (GT)13 107-132.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cATgTTgATAAccgAcTcgcAg at 80 length 22 bases, Tm 65
Reverse primer details :
TgAAAcTAggggccAcgAATAA at 137 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 79 bases.



Length of sequence : 275  Consensus sequence is :

..

ACGCAGCTGG TTTGTTCCCT GTGTTGTTGC TTGAGAAAGT TATCATTAAC 
CTGGTGTATA TGTCGAGGTC CCCAATTGCA TGTTGATAAC CGACTCGCAG 
CTAAGGGTGT GTGTGTGTGT GTGTGTGTGT GTGCGTTATT CGTGGCCCCT 
AGTTTCAAGT TGCAAATCAA CACGCAACTA CGGGGACGTG TGTGTGTGTG 
TGTGTGTGTC GTCGGTCCCC AATTGCATGT CGATAACCGA CTGCAACTAG 
TGTGCGTGTG TGTGTGTGTG TGTGT

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wmc248(5d5.3) Plate reference : 5d5.3
WMC locus number : WMC248 Length of sequence : 460
Last updated : Thu, Aug 28, 1997 at 10:57 am
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Third insert only shown here (without linkers). Bases 1-319 ( complement) match part of 7a3 (3). (CA)19 228 to 265. Primers shown are for third insert, the forward primer is within the homologous region to 7A3(3) but has 2 internal bases different. To exploit this must use anneal at close to Tm (try 61 first).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
gTATgcATgcTcAgTgTgcTAT at 82 length 22 bases, Tm 60
Reverse primer details :
TTAAAAgcTgTcAcgAgcgAAg at 304 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 243 bases.



Length of sequence : 460  Consensus sequence is :

..

ACGAGTTCGT TGGTTTCAAC ATAGCCCTAC CCGTAGCGAT CCACTGCTTC 
CACCCTGATT GTGCCGCTGC GTGAAATTTT TGTATGCATG CTCAGTGTGC 
TATGATGTTC TATATTGATT GTGTCAACTA TATAAGTTTT TACTGAGCAT 
CATCAATGCA TATGGCTGAA AGATGTATTT ACGAGGATCG ACCGATGG!G 
TCTCTCTCTC TCTCACACAC ACACAAACAC ACACACACAC ACACACACAC 
ACACACACAC ACACAGACGC ACACACGCAC AAGTGGGACC CATTGAATTA 
TTTCTTCGCT CGTGACAGCT TTTAATTAGG TTCCACTTTA CTCTAACTTT 
TTTCTTAAAT TATTAATTGA GCTCAGTGCA TTCAAGAAGT TGTAGAGAAG 
CCTTGTTTGG GACTTTCCGG CGTCGTTGAA TGTGGGCTGA GTAGCCATGT 
TAGGTTGTAC

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wmc(5e5) Plate reference : 5e5
WMC locus number : WMC Length of sequence : 0
Last updated : Thu, Aug 21, 1997 at 3:13 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : No insert No substantial microsatellite.
PCR Comments :



Length of sequence : 0  Consensus sequence is :

..

TNCCTCGAGG CGCGCCTCGA GGGATCCTCT AGAGTCGACC TGCAGGCATG 
CAAGCTTG

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wmc(5f5) Plate reference : 5f5
WMC locus number : WMC Length of sequence : 441
Last updated : Tue, Apr 22, 1997 at 20:57
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 5' and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 441  Consensus sequence is :

..

CTCATGCGTC ACATCATGGG AAATGCTAAC GAGGCGGCCC GCGCCTTCCC 
CATAAAAAAG GAGAACCGTA GGCGCGACGC CCATTTACCC TCTGTGCCTT 
CTTCAGTCTC GGAACCGCCG TTCTTCCGCT CTTCCTTCTC AAGCCCTCGC 
TTCCGCTCGC CGCCGCCGCC GCGCCCTTGT TGTCGCTCAT CCCTCGTCTC 
CCTCAGCCGC CATGGCACCC AAGACCAGCA AAGGCAAGGG AACGGCCAAG 
GATGCCGGGG GAAGGATGCG CCGGAGAGCG AGTTGGCGGC GCGGCGGACG 
CAGCTCGCCA ACTTCCCTTC GACGGTCGAC GCGGTCCACC TCCGGGACTA 
CTTCAAGCCT CTGTGGGAGT GCAAGATGAA GGGGCACCCC GCCATGCGCA 
TCATCCCCGC CAGTTTCGCC GAGGCCGGCC CGAATCGGTA C

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wmc(5g5) Plate reference : 5g5
WMC locus number : WMC Length of sequence : 348
Last updated : Thu, Aug 21, 1997 at 3:14 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed at Rsa1 at 5' and to insert at 3' end. Member of 1g1 family. No substantial microsatellite.
PCR Comments :



Length of sequence : 348  Consensus sequence is :

..

GTACAGATGA TAAGTTTGCT GACTACTAAA GTAAAGTGGA ATTTGTCCAA 
GTTATGCAAG TAAATAAAGG TGATTGTTTA TCATATGGGT AAGGTTGGAT 
GAAGGGTGGT AGGAACAAAT GCTCCGCCTA GAGCATGTGT GTGTGAGATG 
GAGTCTTAGT GTGTGTGTGG TGTGTGTGTG TGTGAGAGAG AGAGAGATGG 
AGTATTAGTG TGTGTGTGGG GGGGGGTGTG CGTGCGTGCG TGTGTGTAAG 
TGTGCATGTC TTCATCATAG AGAGGTGATG TGTATGGCAG AGGCCACCAA 
CGATGGGGTG CGAGAGAGAA AATGCCCGTA GAGAGGGAAG AGAAGGTG

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wmc(5h5) Plate reference : 5h5
WMC locus number : WMC Length of sequence : 405
Last updated : Tue, Apr 22, 1997 at 21:00
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. No substantial microsatellite.
PCR Comments :



Length of sequence : 405  Consensus sequence is :

..

CGTCGTGGAC TAACCCACCA GGAGGGGCCG ATATGTGGGC CTATCTTTGA 
AACCCTAATT ACCTAAACCT AAAGCGCTCA ATTTCATCTC GAGTCCAAAC 
TTTGCCTCAG CAGCCAACCA TCCAACCCTT CTTTCTCTCT TGCCCCATTC 
TCCATGAGAC CACGACTCCA TGCCTCCACC AGATCAAGCC GCCGCCGCCG 
CCTCGATAGC CGTCGCAAGC ATGTGCTCAC TACCAAAGAT GCTACTCCGA 
CAACCCCATC TCGTTGATCC GCTCTTCCCA AAGCTCCCTT GTTCCCCATC 
ATCGCTTGCG CCGCCCGATA GTGTAAGTTG TTGATGGCGA CTCGCCATCC 
ATGTTTCGTG ATGCCCCTCG ACCCTTGGGC TTGCGTCACC GCTGTGCAGC 
CTCGC

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wmc(5a6) Plate reference : 5a6
WMC locus number : WMC Length of sequence : 323
Last updated : Thu, Aug 21, 1997 at 3:17 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at b5' end and to rsa1 at 3' end. Bases 1-191 (complement) match part of 5b7. No substantial microsatellite. Bases 2 to 323 show homology to 12h1 bases 1 to 322 (322 in longest run, maxScore = 263). Bases 72 to 140 show homology to 12h3 bases 193 to 261 (complement) (69 in longest run, maxScore = 153).
PCR Comments :



Length of sequence : 323  Consensus sequence is :

..

CAGGGTCGAG GAGCAAAGCT TAGTGCGGCT GCCGCCTGAA GTTAGACCTC 
CACTATCCCG CCATCGACCG CGATGGATGT GCTCGAATCA CGCCGCCGCC 
GACCTCGATG GTTGAGCTCC TCCTATCTCC CGCCGCAGAC CGAGGACTGA 
CCTCCTATGA CGCCGCCACC GCCCACCGAC CGAGGATTGA CCGCTTCTCC 
GGTAGCCGCT GCCTAGGCCG CCGCCATCTC TATATGCAGA GCGGCTGTTT 
TTTTACGGAT ATCCGTAGAG CAGCTGTTTA CACGGTATCT CACCGTAACT 
CGGTTATATG GACAGGGGAG TAC

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wmc(5b6) Plate reference : 5b6
WMC locus number : WMC Length of sequence : 220
Last updated : Thu, Aug 28, 1997 at 11:09 am
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)10 1 to 20, (GA)19 27 to 64, (GA)17 67 to 100. No room for primers.
PCR Comments :



Length of sequence : 220  Consensus sequence is :

..

GAGAGAGAGA GAGAGAGAGA GGGAGGGAGA GAGAGAGAGA GAGAGAGAGA 
GAGAGAGAGA GAGAAAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
GTCCGATTCG CCGTTAAGAC CGAGCACGAT ACGCATGGAC GTGTGGACGA 
ACGTGTTGTT TTCCATACTA GGTAGAAATC ATATGAATTT GTTGAACAAT 
ACTGCAGTTT AATGCATAGT

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wmc(5c6) Plate reference : 5c6
WMC locus number : WMC Length of sequence : 295
Last updated : Thu, Aug 21, 1997 at 3:39 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 5' and and to Rsa1 at 3' end. Matches 5d11. No substantial microsatellite.
PCR Comments :



Length of sequence : 295  Consensus sequence is :

..

GATGAGTAAC TTCGACCAGA GAGTCGCGCT CTTCCAAGGA AACAACTCCG 
GCGGTGGGTT CAAGTCGTCT GCTAACACCG CTTCACGTGG CCGCGGCGGC 
GGTGGCGGCG GCTCCTCACG CTACACCAAC CCTAAGGGAC GCCGCAACAA 
CTCCAGCAAG TCCCATCCAG ATGCTGTCCG CTGCCAGATT TGTGGCAAGC 
TTGGACACTC GGTGAGGGAC TGCTGGTATC GCTTCCAGGA GGACAGCGAC 
TCGTCCGGTG ATGAGAAGGT CGCTGGAGGA GCGGATGGCT CGTAC

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wmc249(5d6) Plate reference : 5d6
WMC locus number : WMC249 Length of sequence : 514
Last updated : Lun 4 Jan 1999 at 15:33
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 269-510 (ie. 5' side of microsatellite) match part of 7f8. Bases 270-512 (ie. 5' side of microsatellite) match part of 4H6 (complement) .(GT)19 232 to 269, (CT)20 192 to 231. Reverse primer is in region with 2 insertions, 1 base change relative to 7F8, does match 4H6. Bases 356 to 427 show homology to 2h5 bases 88 to 159 (complement) (72 in longest run, maxScore = 174). Bases 19 to 125 show homology to 20d8 bases 124 to 230 (complement) (107 in longest run, maxScore = 129), duplicate with 20d8, 5d6 has priority. True duplicate with 2h5, 5d6 has priority. Duplicate with TW7a02.2, 5d6 has priority.
PCR Comments : 61C anneal, product;
Forward primer details :
AAAATTgTcAATcccTcAAccc at 34 length 22 bases, Tm 63
Reverse primer details :
gAgcTTTTgAAgATggAgcAgA at 325 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 313 bases.



Length of sequence : 514  Consensus sequence is :

..

GTACAAAATT ACCAATCTCT CTCTCCCCCG CACAAAATTG TCAATCCCTC 
AACCCATGAG ATCCTTCCCC TCTCGTCCAT GTTTTTCCAG CTCTCTCATC 
AAACATGTTG TCTCTTCTCC TTCCATGTAT ACAGAATCAC GTATATTACA 
TGTCTCCATC TTTCTCACAG ACCTAACTTC TTCCTCTCTT TCTCTCTCTC 
TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTC GTTAGATTTG TCTCCTACTC TCTCGTCCAC 
ATACATACAA TTTTTCCCGC CCTATCTGCT CCATCTTCAA AAGCTCTCTC 
TGCATGTTTC ATTCACACAA TGGGATATGT CAAAATGTTG CTTCTATAAT 
GGCTGATGTA GAGTTTTGGT TGTTTTTATT GCATCCTACA AAGTAATAAC 
TATGAAATGC ATTTAGACTC TCATTTGACC GGGATTACGT GAGTTTGAGC 
ATGTTGTGAA GTAC

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wmc(5e6) Plate reference : 5e6
WMC locus number : WMC Length of sequence : 458
Last updated : Mar 20 Juil 1999 at 14:11
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 1-294 match part of 7e1, bases 18-134 match part (nearly all) of 13G3(2) (Complement). No substantial microsatellite. Bases 48 to 79 show homology to 24h4 bases 293 to 324 (complement) (32 in longest run, maxScore = 184). Bases 124 to 153 show homology to 20h8 bases 112 to 141 (complement) (30 in longest run, maxScore = 101). Bases 183 to 207 show homology to 10d3 bases 256 to 280 (complement) (25 in longest run, maxScore = 179). Near duplicate with 10d3, 5e6 has priority.
PCR Comments :



Length of sequence : 458  Consensus sequence is :

..

GTACCGTCAT CGGGTTCATG GGGAAGGCAC TCCTCCACTC GTCGACGTCG 
ATCTCCTCGT CCTCGGGGCC CACCTCGACG CCCTCCGTGC CGCCGTCGCC 
GCCGCCTTCG ACCTCGTCAT TAGAGGAGAG CGCGATAACC TCGCCGCCCT 
CCGTGTCGCC GTCCTCGCCG CCTTCTACCT CATCATCGGA GGAGAGCGCG 
ATAACCTTGC CTCCCTCTGC GCCCGCAAAG ATCGCCCGCT CCGCCTCCGT 
GAGCTATGAG TGCTGCCGAT GGAGCTCTTG CATATAGGCC TCGTCGGCGG 
CCTCGGCCTC GAGACGCTCC CACGCCTTGC GGTCCTCCCG CGCCATAGCC 
GAGCTCACCA CCCCTGGCTC CGGCGGAATG AGGTGGACCG GACGTCTGCC 
GAAGGGGAAA TTGAGCCTAG CCGCCGCGTC ATGGTAGCGG ACCTGTCAGC 
GGTCGTAC

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wmc(5f6) Plate reference : 5f6
WMC locus number : WMC Length of sequence : 680
Last updated : Thu, Aug 21, 1997 at 4:46 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (GT)11 651 to 672. No room for primers.
PCR Comments :



Length of sequence : 680  Consensus sequence is :

..

GTACATGCTA CTTGCCTCTA TGGACGGTGT GCGCTAATGT GTGCATCACG 
TATGATATAA TGATCAAATT GATCTATCAA CACAAGTTCG ATGACCACCA 
ATGCATTTTA CTCGGCAATA AATGACCAGG ACTGAAGGGG AATCCAAATT 
TCCTTCGTCT TCTGTCCTTG AGCTCTTGAC AAACCAATGC ACTATACGGT 
TGTCCGGCCA CTCCACCCCA GAGCTCTCAC CAAGCGTCGT TCATGCCACC 
GCGCTGCACA CTAACCGGTA AGGAAGCGAT GGCATCCCGC CGCGCCGAGT 
TCCTTGCCGC CCACGACCGC ACACAAAATG GTAGGGAAGC GATGGCATCC 
CGCCGTGCCG AGTTCATCAC CGCTCGTGAC CTCACACCTA CGTGGGCGGA 
ATTTGAAGCT GCCAAGGCCA AATGGGCGGT AGAGCAAGAG GCGGCCAAAG 
CCGCACAGAG GGAGCGGAGA AGTCAGAAAG CACACAAGAA AAGAGAGGCA 
CGCCGCCGCA AGAAAGGAGA GGACGCACGC GCTGCTGCGG AGAGGTCGGC 
GGAGATCCAA GCACGGTGGG GTGTCGCCGA CAAAGCCGGG AAGGAGAACG 
ACGGCGTCTG GCCAGTATGT GAGAAGTAGT AGTAGCAGTA GTAGTAGTTA 
GTGTGTGTGT GTGTGTGTGT GTCTGAGTAC

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wmc(5g6) Plate reference : 5g6
WMC locus number : WMC Length of sequence : 238
Last updated : Thu, Aug 21, 1997 at 4:47 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' and to insert at 3' end. Member of 1g1 family. (GA)8 186 to 201.
PCR Comments :



Length of sequence : 238  Consensus sequence is :

..

GTACAAATGA TAAGTTTGTT GACTACTAAA GTAAAGTGGA ATTTGTCCAT 
GTTATACAAG TAAATTAAGG TGATTGTTTA TCATACGGGT AAGGTTGGAT 
GAAGGGTGGT AGGAACAAAT GCTCCGCCTA GAGCATGTGT GTGTGAGATG 
GAGTATTAGT GTGTGTGTGG TGTGTGTGTG TGTACGAGAG AGAGAGAGAG 
ATGGAGTCTT AGTGTGTGTG TGTGTTTGTG TGTGTGTG

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wmc(5h6) Plate reference : 5h6
WMC locus number : WMC Length of sequence : 231
Last updated : Thu, Aug 21, 1997 at 4:49 pm
Owner : Sharp, Camden Date at Agrogene : 21.4.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. Matches 7d10, 3d2. (CT)16 46 to 77, (CT)19 127 to 164.
PCR Comments :



Length of sequence : 231  Consensus sequence is :

..

GAGGCGCGTC CAACACACAA ACCTTGTTGG TCCCTCTCCC TCCATCTCTC 
TCTCTCTCTC TCTCTCTCTC TCTCTCTGAC AATCTTCAGT TCTATCGCAC 
GCACCAAAAA CACAAACCTT GTTGGTCTCT CTCTCTCTCT CTCTCTCTCT 
CTCTCTCTCT CTCTGACAGT CTTTAG

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wmc250(4A1) Plate reference : 4A1
WMC locus number : WMC250 Length of sequence : 217
Last updated : Thu, Aug 28, 1997 at 2:30 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. (GA)18 121 to 156. Bases 1-148 (complement) (ie 5' side of microsatellite) match part of 4H4 . Forward primer contains single base insertion difference with respect to 5H4.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
ATgcTTATggAccgTgAcAgAg at 91 length 22 bases, Tm 63
Reverse primer details :
AgTgcAAAggcgccgATTTATg at 182 length 22 bases, Tm 69
Expected size of amplified fragment (Chinese Spring=) 113 bases.



Length of sequence : 217  Consensus sequence is :

..

AATCTAATGA TCATTCCAGA TGTAAATTTT TTCTCCACAT ATACCTAGTC 
CAAACCTTTT CGAGGTTTGC ACCTTTTCAC GAACATCCAT ATGCTTATGG 
ACCGTGACAG AGTCCCTGAC GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
GAGAGAAAAG CGGCGGGGAA GGACAGACAC CCATAAATCG GCGCCTTTGC 
ACTCCCTCCT CGCTGCA

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wmc251(4b1) Plate reference : 4b1
WMC locus number : WMC251 Length of sequence : 171
Last updated : Thu, Aug 28, 1997 at 2:49 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. (GT)17 39 to 72. Struggled to get these primers, use at close to Tm (may get mispriming at low temperature).
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
gAAgAgTgAgTgTgcAAAAg at 18 length 20 bases, Tm 55
Reverse primer details :
cgAgAgTgATggcgTAgATT at 140 length 20 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 142 bases.



Length of sequence : 171  Consensus sequence is :

..

GCAGTGTGTG TGAAGGAGAA GAGTGAGTGT GCAAAAGAGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGAAAGAGA AAAGGACAAC AAACCATAAA 
ACAGAGAGAG TGTGTGTGTG TGTCTGTTAC ATTTGCAACA ATCTACGCCA 
TCACTCTCGA GAATCAACGG T

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wmc(4c1) Plate reference : 4c1
WMC locus number : WMC Length of sequence : 561
Last updated : Mon, Aug 25, 1997 at 5:09 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 160-384 (and beyond) match part of 5A2. No substantial microsatellite.
PCR Comments :



Length of sequence : 561  Consensus sequence is :

..

GTACGACTCC TTCAACCCCG TTCTCTTGAA CGCTTCCGCA TAGCAATCTA 
CAAGGGTATG TAGATGCACT CTCCTTCCCC TCGTTGCTGG TTTCTCCATA 
GATAGATCTT GGTGACACAT AGGAAAATTT TGTATTTCTG CTACGTTCCC 
CAACACTTCC CCCCTCTACT CTCTTGTAAT GAATTGAGTT ACCCGTTTGA 
AGCTATCTTA TCGGATTGAG TCTTTAAAGA TTTGAGAACA CTTGATGTAT 
GTCTTGACGT GCGTATCTGT GGTGACAATG GGATACCACG TGATTCACTT 
GATGTATGTT TTGGTGATCA ACTTGTGGGT TCCGCCCATG AACCTATGCA 
TAGGGGTTGG CACACGTTTT CGTCCGTGAT TCTCCGGTAG GAACTTTGGG 
ACACTCTTTG AGGTCCTTTG TGTTGGTTGA ATAGATGAAC CTGAGATTGT 
GTGATGCATA TCGTATAATC ATACCCACAT ACCCACGGAT ACTTGAGGTG 
ACATTGGAGT ATCTAGTNAC ATTAGGGTTT TGGTTGATTT GTGCCTTAGT 
GTTTATTCTA G

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wmc(4d1) Plate reference : 4d1
WMC locus number : WMC Length of sequence : 413
Last updated : Mon, Aug 25, 1997 at 5:16 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end. Near duplicate (bases 150-337 and elsewhere) of 7E1, but with some differences. No substantial microsatellite.
PCR Comments :



Length of sequence : 413  Consensus sequence is :

..

GTACTACCGC TGGCAGGTCC GCTACCATGG CGCGGCGGCA AGGCTCAATT 
TCCCCTTCGA CACAAGTCTG GTCCACCTCG TTCCATCAGA GCCAGGGGTG 
GTGAGCTCAG CTATGGCGCG GGAGGACCGT GAGGCATGGG AGCGCCTCAA 
GGCCCACATG AAATAGCTCC GCCGGCAGCA CCCGGAGCTC GTGGAGGCGG 
AGCGGGCGAT CTTTGTCGGC GCGGAGGGTG GCGAAGTTAT CACGCTCTCC 
TTCGATNACN AGGTANAANG CGGCAAGGAC GGCGGCGCGG AATGCCGCGA 
GGTTATCGCG CTCTCCTCTG ATGACAAGGT TGAAGGCGGG CGGCGACNAT 
TGCGCCGCAA GGCGTTNAGT GGGCCCCGAC GACCAAGAGA TCCACGTCCG 
ATAAATGGAA GAA

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wmc(4e1) Plate reference : 4e1
WMC locus number : WMC Length of sequence : 85
Last updated : Tue, Aug 26, 1997 at 1:24 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)13 5 to 30. No room for primers.
PCR Comments :



Length of sequence : 85  Consensus sequence is :

..

GTNTGTGTGT GTGTGTGTGT GTGTGTGTGT ATGGATTCAT GTGTATGCGA 
GCCCTCAAAC TTAGGTCTCG AATCTCACGA TGTAC

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wmc(4f1) Plate reference : 4f1
WMC locus number : WMC Length of sequence : 570
Last updated : Mer 14 juin 2000 at 10:34
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. No insert - PUC sequence only. No substantial microsatellite. Bases 28 to 392 show homology to 10b8 bases 268 to 632 (complement) (365 in longest run, maxScore = 277). Bases 24 to 349 show homology to 10h5 bases 13 to 338 (326 in longest run, maxScore = 264), duplicate, 4f1 has priority. Bases 32 to 60 show homology to 20c11 bases 1 to 29 (complement) (29 in longest run, maxScore = 28). Bases 23 to 419 show homology to 22a3.1 bases 52 to 448 (397 in longest run, maxScore = 408). Duplicate with 22a3.1, 4f1 has priority. Bases 102 to 170 show homology to TW3g03.2 bases 347 to 415 (69 in longest run, maxScore = 55). Bases 100 to 128 show homology to TW1d06 bases 331 to 359 (29 in longest run, maxScore = 103).
PCR Comments :



Length of sequence : 570  Consensus sequence is :

..

GATTCTNGAG GCGCGCCTCG NAGGGATCCT CTAGAGTCGA CCTGCAGGCA 
TGCAAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA 
GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 
ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 
TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 
ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
ACGCAGGANA GAACATGTGN AGCAAAAGGC CAGCAAAANG CCAGGAACCG 
TAAAAAGCCG CGTTGCTTGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
GCATCACAAA AATCGACGCT CAAGTCAGAG TTGGCGAAAC CCGACAGGAC 
TATTAAGATA CCAGCGTTTC

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wmc252(4g1) Plate reference : 4g1
WMC locus number : WMC252 Length of sequence : 206
Last updated : Thu, Aug 28, 1997 at 2:59 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. (CA)14 128 to 155.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TcAcTATgcTcAcTcTTcggcT at 93 length 22 bases, Tm 63
Reverse primer details :
cTccTcgTgTTccTgATATgcT at 179 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 108 bases.



Length of sequence : 206  Consensus sequence is :

..

CGTGTCTGAA ATTAGCTTTC TCATACGTGG GTTACGCAAC CAACCAAGTG 
AAATCTCAAT TTGAGTTTCT CATTTGAATC TCAGTTACAA TCTCACTATG 
CTCACTCTTC GGCTCACACT GACTATACAC ACACACACAC ACACACACAC 
ACACACTGAG TTAAGACTAC ACTCACTAAG CATATCAGGA ACACGAGGAG 
GACGAC

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wmc253(4h1) Plate reference : 4h1
WMC locus number : WMC253 Length of sequence : 158
Last updated : Wed, Mar 18, 1998 at 2:29 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. (CA)13 63 to 88. Bases 163-232 match part of 11c4. Reverse primer avoids this. Bases 158 onwards were vector sequence (removed 18.3.98).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TcAccgATTgTAAATAgcAccA at 10 length 22 bases, Tm 62
Reverse primer details :
gATAAATcAcTTcgggTgAggg at 126 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 138 bases.



Length of sequence : 158  Consensus sequence is :

..

ATAGCCATTT CACCGATTGT AAATAGCACC ATCCTTTCAC TGTGTGCCTG 
TGTGCCTTCT ACCACACACA CACACACACA CACACACACT AAGCGAGTCC 
GGCGTATTGA GGCCGCTTGA AGGCCCCCTC ACCCGAAGTG ATTTATCAAA 
ATTCTAGT

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wmc(4a2) Plate reference : 4a2
WMC locus number : WMC Length of sequence : 184
Last updated : Mon, Aug 25, 1997 at 5:20 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. Member of 1g1 family. No substantial microsatellite.
PCR Comments :



Length of sequence : 184  Consensus sequence is :

..

GTAAAGTGGA ATTGTATGTT ATACAAGTAA ATTAAGGTGA TTGTTTATCA 
TACGGGTAAG GGTGGATGAA GTGATAGGAA TCAGATAGCT CTCCCCCTAG 
AGCATGTGTG TGTGAAGGTG GAGTCTCAGT GTGTGTGTGT GTATGTGTGT 
GTGTATGGAG AGAGAGAGAG AGTGAGCTTA GATT

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wmc254(4b2) Plate reference : 4b2
WMC locus number : WMC254 Length of sequence : 200
Last updated : Tue, Aug 26, 1997 at 1:29 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. (CA)28 31 to 86. Forward primer is not ideal.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AgTAATcTggTccTcTcTTcTTcT at 2 length 24 bases, Tm 58
Reverse primer details :
AggTAATcTccgAgTgcAcTTcAT at 171 length 24 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 193 bases.



Length of sequence : 200  Consensus sequence is :

..

CAGTAATCTG GTCCTCTCTT CTTCTCAAAA CACACACACA CACACACACA 
CACACACACA CACACACACA CACACACACA CACACATGAC ACATGTGGTA 
AGCAATCCAA ATAATTAAAA CACACACACA CACCCAGCGA TGCAGTCGAC 
ACGAGACTAC CCCCCAGTCA ATGAAGTGCA CTCGGAGATT ACCTTTTGGT

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wmc(4c2) Plate reference : 4c2
WMC locus number : WMC Length of sequence : 228
Last updated : Fri, Jun 27, 1997 at 19:53
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to insert at 5' and 3' ends No substantial microsatellite.
PCR Comments :



Length of sequence : 228  Consensus sequence is :

..

CTGCCGGGGA AATGCCGGAG GCGGCTGCGG CGGNAAAGGA GGCGGTGGAG 
AGGAGAGACA TGGGAGGCGT AGGGAGAAGA GAGGATCTGG GTGAGGGTGC 
AGCTTCGGAG CCGGAGCATG GCGGCGGCGG CNAGGGGGTG GCCGGTGGCG 
GCGACTAAAA GTCAATGAGA AGAGCTAGCG TAACTGCGTG ACTGCATGAA 
CGGTGATCCA TCGAGTGTCT ATCTTGTC

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wmc(4d2) Plate reference : 4d2
WMC locus number : WMC Length of sequence : 133
Last updated : Wed, Jun 18, 1997 at 5:24 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. No substantial microsatellite.
PCR Comments :



Length of sequence : 133  Consensus sequence is :

..

TGCAGGTCGA CTCTAGATAT CACTCGATGA TCTACACGTA ACATTAGACT 
GAGGCACACT TATATTAGTT ACATAGGCTA CGATACGAAT CTGTGCAGCA 
TGTGACCGAC TAGCTACATG AGACATCTAC GAC

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wmc(4e2) Plate reference : 4e2
WMC locus number : WMC Length of sequence : 270
Last updated : Mon, Aug 25, 1997 at 5:22 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end, sequence begins with EcoR1 at 5' end in vector. Bases 169-270 complement match part of 5d5(1). No substantial microsatellite.
PCR Comments :



Length of sequence : 270  Consensus sequence is :

..

GAATTCTGGA GGCGCGTCCT AAACTGCACC AGAGGCTTCC CGTTTGGTTA 
TTTTTCTTTT TCTTTCAGGA CTTCAATCTC TGGAAACTCT GTCCGGCAGC 
TCTATTGCCG AACACATGAT ACAGCAACCA AGGAGGCTGA CGACTACGGA 
ATTCCCAGGT GGATTACAGA ATTCCCAGGT GGATTACAGA TTTCACATAA 
TTCCTCAGCT CGCCCTTGGA AGGGACATAG TCCTACTCTT TTGCTCATTA 
CACTATCTGT ATCACTCTGC

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wmc(4f2) Plate reference : 4f2
WMC locus number : WMC Length of sequence : 548
Last updated : Mon, Aug 25, 1997 at 5:27 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end, sequence begins with EcoR1 site in vector. Duplicate of 1D7. No substantial microsatellite.
PCR Comments :



Length of sequence : 548  Consensus sequence is :

..

GATTCNTCGA GGCGCGTCCA CCGCATCATC TCCTGGTGAT GCAGGTGTGA 
CATTCCCCAC TCCTTCAGAA CGTGCGCCGT CTTCAGGTTT CGCGTGCTGC 
TCCGCGTCGC CGGCTGGTGG TGCGTTCCAC GCCGGTGCTT GCGGAAATCG 
ACGACGAAGG GTGTCGTAGT CCTCCCAAGT CGCCGCCGTC TCATCGTTGC 
GCC

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wmc255(4g2) Plate reference : 4g2
WMC locus number : WMC255 Length of sequence : 304
Last updated : Thu, Aug 28, 1997 at 3:44 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end. Sequence possibly starts with EcoR1 at 5' end. (GT)4(TT)(GT)7(AT)(GT)5 at 24-59. Very difficult to find reverse primer with high enough Tm, forward primer may overlap linker.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TcgAggcgcgTggATAAc at 6 length 18 bases, Tm 65
Reverse primer details :
cAccTTgcATATATgAcTgAgc at 64 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 76 bases.



Length of sequence : 304  Consensus sequence is :

..

GANNCTCGAG GCGCGTGGAT AACGTGTGTG TTTGTGTGTG TGTGTGTATG 
TGTGTGTGTG CTCAGTCATA TATGCAAGGT GTTGTTGAAC TACAAATTGA 
TGTATTTGCC AATTTCATTT CATATTGCAC CCAAACTGGC AAATAGATAA 
ATCTGGAAAA ATATTAAATA CACGTAAATA ATACATATAG CACCGAAACA 
AGGTAAATAC ATAAATATAG CACCGAAACA GGACAAATAC ATAAAGTTGA 
GAAAATATCA GATACAAGCG AGTGGTGGCT TGTGTTAATT AGCCGCAACT 
CCAC

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wmc256(4h2.1) Plate reference : 4h2.1
WMC locus number : WMC256 Length of sequence : 324
Last updated : Thu, Aug 28, 1997 at 4:32 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Small linker like sequence at 234-241 in original ie. multiple inserts (2) in this sequence, this record shows first insert only (up to but excluding linker). No trimming done to sequence at 5'. (CA)12 65 to 88.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
ccAAATcTTcgAAcAAgAAccc at 37 length 22 bases, Tm 64
Reverse primer details :
AccgATcgATggTgTATAcTgA at 132 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 117 bases.



Length of sequence : 324  Consensus sequence is :

..

GATCCTCGAG GCGCGTGGCT CTCCATAAAT AACCGGCCAA ATCTTCGAAC 
AAGAACCCAC AACTCACACA CACACACACA CACACACATC ACAGAAGAGC 
ATAGTTCGAT TGAACACTCA CAAAGTTTGA ATCAGTATAC ACCATCGATC 
GGTCTCTCCA TGGCCACGAT AATAAGGAAG CTTGTGGCGG GCATGCTCTT 
GGTTCTCGTG GCCACGTCCG GCTCATCATG GT

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wmc(4h2.2) Plate reference : 4h2.2
WMC locus number : WMC Length of sequence : 324
Last updated : Tue, Aug 26, 1997 at 1:32 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Small linker like sequence at 234-241 in oringinal. No trimming done to sequence at 3', this record shows second insert only (not including linker) it matches 2E9.
PCR Comments :



Length of sequence : 324  Consensus sequence is :

..

TGCACAACAC ACACAGACAC ACACCAACAC CCTTTTATCT GGATCCTCAT 
CTCTCCCCAT GTCCGCATGT GTATCTCACA CA

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wmc(4a3) Plate reference : 4a3
WMC locus number : WMC Length of sequence : 70
Last updated : Fri, Jul 4, 1997 at 4:50 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 70  Consensus sequence is :

..

GTAGTGGAGC CTTCGTGACT CAGCGACTGA GAGGCACGCG TACTGGGTTG 
GACTGGAACG CCTGCTCTTG

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wmc(4b3) Plate reference : 4b3
WMC locus number : WMC Length of sequence : 217
Last updated : Tue, Aug 26, 1997 at 5:29 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end. Bases 72-217 (ie. 3' side of small microsatellite) match part of 3c11. (CA)4c(CA)(CG)(CA)(GA)(AC)5(CC)(AC)3. Bases 165-217 (complement) match part of 2D8. Too difficult to design primers.
PCR Comments :



Length of sequence : 217  Consensus sequence is :

..

NGNANTCTTC GAGGCGCGTG GATATCGCAC ACGGCCATGG CCAGCTGCAC 
GCACACACAC CACGCAGAAC ACACACACCC ACACACAGCT CGGCCATACC 
CGGCCGTAGC AGCGGCGGCG GCGACGGACA ATAGCGGCAG CAACATGAGC 
GACGCAACAA GCAGTGGCGG CAGTGGCAAA CAGCAGTGAC AGCACAAGGG 
GCAGTCAGCA GCAGTAC

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wmc257(4c3) Plate reference : 4c3
WMC locus number : WMC257 Length of sequence : 374
Last updated : Lun 30 oct 2000 at 16:59
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end. (GA)14 85 to 112. Bases 22 to 45 show homology to TW2f12.1 bases 57 to 80 (24 in longest run, maxScore = 30). Bases 56 to 79 show homology to TW6c10.1 bases 3 to 26 (24 in longest run, maxScore = 23).
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
ggcTAcAcATgcATAccTcT at 20 length 20 bases, Tm 57
Reverse primer details :
cgTAgTgggTgAATTTcggA at 329 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 329 bases.



Length of sequence : 374  Consensus sequence is :

..

GAATTCTTCG AGGCACGTGG GCTACACATG CATACCTCTG AAAAATTTCG 
CAGGAGAAAA CAACAGATTT CTCATCGCGA GAATGAGAGA GAGAGAGAGA 
GAGAGAGAGA GAGGGATGGA GAGGGAGAGG GAGAGGGAGG AGAGAGGGAG 
AGAGAGAGAG ACGCCTTACC GAAAAAGGGT TTCCCCCCGC TTTGTATTCC 
AAAGCACCAA CACCGATCAC AGAGCAGACG CTGTGGCGAG CAGCACAACA 
AGCCAAAGAA AAAGAAGAAG AAACAATGCC AACACCGGCA GCTTGACAAA 
GCGCGGATGA CCCACTACCG CTGCGCCCTC CGAAATTCAC CCACTACGAT 
CCTAGGCTCC GATCCTCCGC GTAC

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wmc(4d3) Plate reference : 4d3
WMC locus number : WMC Length of sequence : 173
Last updated : Fri, Jul 4, 1997 at 4:53 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end. No substantial microsatellite. Bases 123 to 141 show homology to 12b2 bases 409 to 427 (19 in longest run, maxScore = 43).
PCR Comments :



Length of sequence : 173  Consensus sequence is :

..

GTACCACCAG GTTGTAACAA AACGATCTCC AGCAAACTAG GAAAATGCAA 
TCATGGCACT ATTTATTACC TTATGAGGAG AAGGCGGCGG CGGCGGCACG 
TCTCTGTTGG AGCGGTGTGG AAGCACAATG CTCCCCAAGA AACCACTAGG 
GCCACCATAA TCTCCTCACG CCN

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wmc(4e3) Plate reference : 4e3
WMC locus number : WMC Length of sequence : 182
Last updated : Tue, Aug 26, 1997 at 1:36 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : No trimming done to sequence. (CT)32 106 to 169. No room for primers.
PCR Comments :



Length of sequence : 182  Consensus sequence is :

..

GATTCCTCGA GGCGCGTGGC ATAACATATG GCTAGTTTGT GGATCTCCTA 
ATCCCTCATT AGGGTTTCCT ACTTCCGTCG CCGATGCCCG TCATTTTAGT 
TGCTTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC 
TCTCTCTCTC TCTCTCTCTC AGATGGCTGC CC

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wmc258(4f3) Plate reference : 4f3
WMC locus number : WMC258 Length of sequence : 460
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
PIC Value : 0.57
Comments : No trimming done to sequence. (GT)26 115 to 166. Bases 251 to 270 show homology to 17c8 bases 101 to 120 (20 in longest run, maxScore = 43). Bases 112 to 166 show homology to 20b7 bases 241 to 295 (55 in longest run, maxScore = 61).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
gcgATgTcAgATATccgAAAgg at 75 length 22 bases, Tm 65
Reverse primer details :
AccAggAcAccAgAAcAgcAAT at 346 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 293 bases.



Length of sequence : 460  Consensus sequence is :

..

ANTCCTCGAG GCGCGTGGAT ACGTGGACTA ACGGTATGGT GCATGGGGTT 
TCGTTGGCCT GCAAAGACAT GTCTGCGATG TCAGATATCC GAAAGGAAAC 
GGAAACGGAA TATTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTAGAA GAGGGCCGAT GTGACAAGTA CTTACCGGGA 
GGGCATCGAC CTCAGCCAAA GACGAAGCCC CGCCGAGCAT GCCAGAGACG 
GAGGACGGGC TGCTATGAGC AGGTGCGATC GCGTGAGCAG GTGCTGGTGC 
GACATTCACC AAGTTGCTCC CGACTGTATA CTACAGTCGG CGGCGATTGC 
TGTTCTGGTG TCCTGGTCCT ATGGGGTCTT AGCACGACGA CTTCCCGACT 
ATATACTTCA ATAATGTTTG TCCGGCCCCG ATGTGGGAAG GGCGACCNAT 
GGCAGTGTTG

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wmc(4g3) Plate reference : 4g3
WMC locus number : WMC Length of sequence : 6
Last updated : Wed, Jun 18, 1997 at 5:26 pm
Owner : Gupta, Meerut Date at Agrogene : 9.5.97 Public Domain : Yes
Comments : no sequence obtained 9.5.97
PCR Comments :
No sequence available for this clone
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