Wheat microsatellite WMC 25/06/01

wmc(3h9) Plate reference : 3h9
WMC locus number : WMC Length of sequence : 257
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. No substantial microsatellite. Bases 202 to 234 show homology to 12c12.1 bases 19 to 51 (complement) (33 in longest run, maxScore = 78). Bases 112 to 141 show homology to 2d4 bases 121 to 150 (30 in longest run, maxScore = 66).
PCR Comments :



Length of sequence : 257  Consensus sequence is :

..

ACATTAAGAG AGAAAAACGC AAGTCCTCTC AAACCATATC TTTCATTCAG 
TCATACTGCA AATTTATATT CATATGATCG AGATACAGTA TTAAACCCAA 
GAAAAAAACT ATTTCCGGCG GCGGCGGAGG CGGCGTGCCG CATCATCGTT 
GTCGTTGTCG TCCTCCGGGA CGCCGTTGTT GAAGTCTTCA ATGCAGCATA 
GAATGTTCTT CACTTGTAAA TCAAACATCA TGTCATCGCG CGATTGACGG 
GCGGGGC

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wmc277(3a10) Plate reference : 3a10
WMC locus number : WMC277 Length of sequence : 304
Last updated : Tue, Sep 30, 1997 at 2:37 pm
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. (CA)18 248 to 283.
PCR Comments : 51C anneal, product;
Forward primer details :
cATAgTgTgAccAAcTAcgAAc at 187 length 22 bases, Tm 57
Reverse primer details :
gTgTgcTAAccTcTATAgTgTg at 280 length 22 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 115 bases.



Length of sequence : 304  Consensus sequence is :

..

AGTAATCATT TATGACCACA AGGAGCTTGA CCTTTACCCT TGACTCTCGC 
ATAACACCAC AGAATCCAAC AAGTCGGTAT TCACGATGCC AAAGTGATCC 
TCTCACGATC ACATAAATAT CAACCCACTT CTTTCATCAA CGAACTGGGG 
TTGTTATTAA GCAAATTATT ATTATTACTG TTGACCCATA GTGTGACCAA 
CTACGAACTG GGCCCATATC CACGGGCGCG GCTATCGATA GATTAAACAC 
ACACACACAC ACACACACAC ACACACACAC ACACTATAGA GGTTAGCACA 
CTGT

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wmc(3b10) Plate reference : 3b10
WMC locus number : WMC Length of sequence : 385
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. No substantial microsatellite.
PCR Comments :



Length of sequence : 385  Consensus sequence is :

..

GCTCATTTAC CCTCCGCATC TGTTCAATCT TGGAGCCACC GTTCCCCTTC 
TTCCTCAAGT GAAAAACCGA AGCTCCCGCC TCCGTCCGCC GCCGCCGCGC 
CTCCATTGCC CTCAATCTCT CATTGCTCCT GATTCCTCAC CTCTTTTCCA 
GCTGCCATGG CACCCAAAAC CAGTAAGGGC AAGGGAGTGG CCAAGGACGC 
CGGGGAGAAG GAGGTGCCGG AGAGCGAGTT GGCGGCGCTT TGGACACAGC 
TCGCCCTTTT CCCCTCGACG GTCGATGCGG TCCACCTCAG GGACTACTTC 
AAGCCTCTGT GGGGGAGGAA GACAACGGGG CATCCGGCCA CGCACATCCT 
CCCCGCCGAT TTCACTGAGG CCAGCCCAAA TCGGT

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wmc(3c10) Plate reference : 3c10
WMC locus number : WMC Length of sequence : 485
Last updated : Wed, Aug 27, 1997 at 10:18 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Duplicate of 6D8. (CA)17 349 to 382.
PCR Comments :



Length of sequence : 485  Consensus sequence is :

..

ACCGTCGGTC TTGTCGGTCT CGGTAACGTG GTTGTACTCG CAAACAGCCC 
ATGACATAAA TGGTAATGCA TGGCAGTAAC AGGCATCTTT AGTGGAATGC 
GATGAGGTGG TATGATCATG TATATGCATC AGGTTAAAGA ATTACTTTTG 
AAGAACAGGT TACAGATATC ATAATCTGTG CTAAGGGTTG AACACACCGG 
GTTCACCCTA TATGCCAAGT CATCGGACTT GTCATAATCT TAAGGTATTA 
ATGAGGGCTA AACCATCCGG GTTTACCCTA TATGTCCAGT CACCGAATTT 
GATCATATTA TTGTGATTAT CATGATTATA ACGACACCTT TTTAACACCA 
CACACACACA CACACACACA CACACACACA CACTTGGTTT ATGCGAAAAC 
TCAGGGCGTA GTTTAAGCAG CACCACCCAA CTGTCCTTGA CCGTGGACAT 
GACTATTCGA ATAGTTTACA CTCTGCAGAG GTAGT

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wmc(3d10) Plate reference : 3d10
WMC locus number : WMC Length of sequence : 236
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. No substantial microsatellite.
PCR Comments :



Length of sequence : 236  Consensus sequence is :

..

GGACTTCTTG CTGTTCCAGC TCCGTTCGAA GATCTCGAGA ACCGGCGAGG 
TGCTACTCCT CCGGCGGGCC TCTCGCTCCG ATGCCTCTCT CCTCCTACCG 
CTGTGAGCCG TTGGGGCGAA CGGAAGAGAC CTGAAGCTTT ATAGAAGCGG 
GCGGCTGTGG TTGAGGCGGC GGCGGCGGCA CAAAGTAGCC GTTGGGGGTT 
CTTTGGTTCT TGGAAAGGAA AGTTTCCAGC TGCGGT

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wmc(3e10) Plate reference : 3e10
WMC locus number : WMC Length of sequence : 0
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No sequence 24.6.97.
PCR Comments :
No sequence available for this clone
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wmc278(3f10) Plate reference : 3f10
WMC locus number : WMC278 Length of sequence : 300
Last updated : Tue, Sep 30, 1997 at 2:52 pm
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. (GT)14 200 to 227. Bases 200-300 (ie. 3' side of microsatellite) match part of 11b12
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AAAcgATAgTAAAATTAccTcggAT at 117 length 25 bases, Tm 59
Reverse primer details :
TcAAAAAATAgcAAcTTgAAgAcAT at 257 length 25 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 165 bases.



Length of sequence : 300  Consensus sequence is :

..

ACATAAAATG AAATTTTTTG GGTTTTATGA CATATAATTT TGTTTTCTAA 
TATCAAATTT TACGTAGTGA ATCAATATGA ATGTAACTAT TGATATTTTG 
AATGTAATTT TTTATGAAAC GATAGTAAAA TTACCTCGGA TGAATAATAA 
CGTATTCTGC ACCCTGGGTG ATGAATAGCA TTACTACATA TACATATATG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTTTT GTCCTCTACA ACATTCACTT 
ATAGCTATGT CTTCAAGTTG CTATTTTTTG AACTAAGTGA ATGTGATCGT

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wmc(3g10) Plate reference : 3g10
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No sequence 24.6.97.
PCR Comments :
No sequence available for this clone
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wmc(3h10) Plate reference : 3h10
WMC locus number : WMC Length of sequence : 287
Last updated : Tue, Aug 26, 1997 at 4:57 pm
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Bases 69-234 (complement) (ie 5' and 3' side of GC rich runs) match mostly part of 6B4. No substantial microsatellite.
PCR Comments :



Length of sequence : 287  Consensus sequence is :

..

ACGCCATGTC GGAGAAATCG GAGAGGCCAG CAGAGAAGGG CTCGACAACT 
ATTGCGGCGC AATCAGCATG GGGCCGGTGT CACCTGCTGT TGTCGGAGTA 
GACGAAGTGG TCGAGGTAGA TGACGGCGAC GCTGGCGACG GGCTGGTGCT 
AGATAAAGAC GAAGGGGGGT GGACAGGCGG CGGCAGCTAC GTGGGTAGCG 
GCGGCAGCGG CCAAAGTAGA GCGGCGGCGG CGGCTAGGTT AGGGGCGCGG 
CGGCGGTGCT CGAAGTAGGC GCAGAACCCT GACTGCC

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wmc279(3a11) Plate reference : 3a11
WMC locus number : WMC279 Length of sequence : 461
Last updated : Wed, Oct 1, 1997 at 2:33 pm
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Multple inserts (2) joined 125-142 by linkers. First insert is a duplicate of 7G6. (GT)15 1 to 30, (GCG)6(GTG)(GAG) at 206-230. Primers are for second insert.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cTcTgAcTcTgAcATcATgggc at 147 length 22 bases, Tm 64
Reverse primer details :
gcAgAgcTgAccgTgcATTAcT at 347 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 222 bases.



Length of sequence : 461  Consensus sequence is :

..

GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT TTACAAAGAT GCGAACCCTT 
GTCCCAGAGG AGGGGGTGGC TTATATAGAG TGCGCCAGGA CCCCTGCTCC 
CCTCCGTTAC ACAGGGTTCA ATGTTAGTCC ACGCGTGGAC TAACATCTCT 
GACTCTGACA TCATGGGCAA CACGAGCGTG GGCATGAGCG TCGGTGTGTG 
CTCGAGCGGC GGCGGCGGCG GCGGTGGAGG GTAGGCCCAG TCCCCCACCG 
CCGACAGCGG GAGGGCTTCC TCCAAGCCGT CCCGGCGCGT ATCCTCCTCA 
TGCTGGCTCT CCTCCAGGGC GCGCCGGTCT TGATCTGCTT GCCAAAAGTA 
ATGCACGGTC AGCTCTGCAC AAACAACATG GTAAACTGAG GATAAATGCA 
TTGTTTTGTC GCGCCTAAAA TGCATTGCTG AATGCTAAAT GCATTGTTTT 
GCCACCTACA G

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wmc(3b11) Plate reference : 3b11
WMC locus number : WMC Length of sequence : 234
Last updated : Lun 5 juil 1999 at 17:22
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Bases 12-89 and 145-183 match part of 2H12. Interrupted microsatellites (GT)6+(GT)6+(GT)6. No room for primers. Bases 95 to 122 show homology to 19d3 bases 53 to 80 (28 in longest run, maxScore = 41). Bases 50 to 74 show homology to 10g1 bases 1 to 25 (25 in longest run, maxScore = 52), true duplicate, 3b11 has priority. Bases 111 to 129 show homology to 10c6 bases 143 to 161 (19 in longest run, maxScore = 56).
PCR Comments :



Length of sequence : 234  Consensus sequence is :

..

GAAACTCAGG GCGGCCTGGG TATGGAGATC GGTGGGTAAG CTGCACGGGC 
AAACCGGCAG ATGTTGACAA TGGAAGCCTT GCGGCGGCGG TGGGAGCGGC 
GGCGGGGACT TTGCTAGGGT TTTGAGCGAA GGAGGGTGTG TGTGTGTGTG 
CGCACGCCCG AGGAAGTTTT GGTGTGTGTG TGTGAGTGTG TGTGTGTGGA 
GGGGGCGCGG GGTGTTTGAG GAAATGACGT GGGT

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wmc(3c11) Plate reference : 3c11
WMC locus number : WMC Length of sequence : 350
Last updated : Tue, Aug 26, 1997 at 5:31 pm
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Bases 158-303 (ie. 3' side of small microsatellite) match part of 4B3. No substantial microsatellite.
PCR Comments :



Length of sequence : 350  Consensus sequence is :

..

AACAGGCCGG CCATTACTGG GCATAGCCAG CCAGCGACAC GCATGGNGCA 
CACACACACC ACACTGCACG CACACACACC ACGCTGCACA AGACACACAC 
ACAACACACG GGCGGCACAC GGTCATGGCC AGCTGCACGC ACACACACCA 
CGCAGAGCAC ACACACACAC ATAGCTCGGC CATGGCTGGC CGTAGCAGCG 
GCGGCATCGA CGGACAGTAG CGGCAGCAAC AGGAGCGACG CAGCAAGCAG 
CGGCGGCAGC GACAAACAGC AGCGACGGCA CAAGGGGCAT CCAGCAGCAA 
TACAACGACA GTAGCAACAG CAAGCAACAT AGCGGCAGTA GCTTGCGAGC

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wmc(3d11) Plate reference : 3d11
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No sequence 24.6.97.
PCR Comments :
No sequence available for this clone
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wmc(3e11) Plate reference : 3e11
WMC locus number : WMC Length of sequence : 230
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. (CA)9 210 to 227. No room for primers. Bases 124 to 135 show homology to 22f6 bases 204 to 215 (complement) (12 in longest run, maxScore = 19).
PCR Comments :



Length of sequence : 230  Consensus sequence is :

..

CACATGCACC AACACACACG CACGCGCACA CACATGCAAC AACACGCACG 
CGCACGTGCA CACACACACA CGCAGACACA CGCAGCAACA CACATGCATG 
CCGCACGCAC ACACGCAGCA ACACACACGC ACGCAACACA CACACATACA 
CAAACACGCA GCAGCAGCAG CACACACGCA GGTAGCACAT AGGCACGCAA 
CAGCACATAC ACACACACAC ACACACACGT

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wmc(3f11) Plate reference : 3f11
WMC locus number : WMC Length of sequence : 163
Last updated : Mer 23 DŽc 1998 at 10:53
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. No substantial microsatellite. Bases 1 to 163 show homology to 2h4 bases 1 to 163 (163 in longest run, maxScore = 126). True duplicate with 2h4, 3f11 has priority.
PCR Comments :



Length of sequence : 163  Consensus sequence is :

..

GGCGGCGGCG GCGGAGACGG GGACGGGCAG TTGTATCCGC AACCGCGAAC 
GGTGTAGACC ATCAGTGCCA TGGCAAGGAG CAGGAGGGCG AGCTTGGACG 
ATGGCGGCGG CGCCATTGCT AGAGCTTTGA TTGAGGAAGC TAGTCGCATG 
TGTATGTGAT TGT

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wmc280(3g11) Plate reference : 3g11
WMC locus number : WMC280 Length of sequence : 159
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Bases 60-159 (ie. 3' side of microsatellite) match part of 8c7.(CT)18 49 to 84. Software struggled to get these primers. Beware - reverse primer is in the duplicated element also in 8C7 - so may get smear. Bases 86 to 145 show homology to 10c8 bases 438 to 497 (complement) (60 in longest run, maxScore = 47), 3g11 has precedence. Bases 114 to 158 show homology to 25h2 bases 1 to 45 (complement) (45 in longest run, maxScore = 45).
PCR Comments : 51C anneal, product;
Forward primer details :
cATgTccAAAgAgTAgAgTc at 2 length 20 bases, Tm 51
Reverse primer details :
TTgTAAcATAcgTgTcTgcT at 123 length 20 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 141 bases.



Length of sequence : 159  Consensus sequence is :

..

ACATGTCCAA AGAGTAGAGT CACACACACG CACTCTGTCT CTCAGAATCT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTTACACA CACACACACA 
CCAGTTACGT GACGCCCACT TAAGCAGACA CGTATGTTAC AATTTGTGCA 
CCCGCGGGT

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wmc(3h11) Plate reference : 3h11
WMC locus number : WMC Length of sequence : 268
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. No substantial microsatellite.
PCR Comments :



Length of sequence : 268  Consensus sequence is :

..

GGAGTTGCAA CTAGGGCAAC TCCACAAAAC TACCATGCCA GTTGCGAGTC 
GAGGATGCAC TTGCAACTGG GAAAACAAAC GGTCGAGTTG TGAGTCGAAG 
GGTGAACTTG CAACAGAGAC AACAACAAAA CTATGGGCCC AGTTGCGAGT 
CGAGGCTGAA CTTGTAACTG GGATGAAGTA AACAATATTC CTAGTTGCGA 
GTCGAGGGCG GACTTGCAAC TGGGATAAAA ATTAGTAGAA GTGAAGTTGA 
GTGCAAAAAC TTCCCTGT

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wmc(3a12) Plate reference : 3a12
WMC locus number : WMC Length of sequence : 309
Last updated : Wed, Aug 27, 1997 at 10:24 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Multiple inserts (2) joined at 220-238 by linkers. Bases 115-219 (complement) match part of 4B9. (GT)11 130 to 151. No room for primers. Bases 53 to 75 show homology to 12h10.1 bases 148 to 170 (complement) (23 in longest run, maxScore = 59).
PCR Comments :



Length of sequence : 309  Consensus sequence is :

..

CTCCTCAGTC CAGGTTTGAT GGAATATTCG TNACCTCTNT CTTCTTGTGC 
ATACTCCATT TGTNTCTCAC TTCCTGAGGN TTCGCACTCG CATGATATTT 
CTATTCTGTA AACATGTGTG TGCGTGTGCG TGTGTGTGTG TGTGTGTGTG 
TTTAACAGCA TGTGTGTGTT AGAGAGAGCG ACACATCGAC CTACTCCTAT 
AGAGAGATGG TGTGTAGTGT TAGTCCACGC GTGGACTAAC NGATGATAAG 
TTTGCNAACT ACTAAAGTAA AGNGGGATNT GTCCATGTTC TACNAGTAAA 
TTAAGGTGA

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wmc281(3b12) Plate reference : 3b12
WMC locus number : WMC281 Length of sequence : 280
Last updated : Wed, Oct 1, 1997 at 3:12 pm
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. (CA)20 48 to 87, (CA)8 90 to 105,
PCR Comments : 51C anneal, product;
Forward primer details :
AcccAgccTcTgcATATTTA at 2 length 20 bases, Tm 59
Reverse primer details :
TTTcATcTTccAcAggAcAA at 244 length 20 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 262 bases.



Length of sequence : 280  Consensus sequence is :

..

CACCCAGCCT CTGCATATTT AGGATGCACA CAACCAACAC AACAGAACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACAAAC ACACACACAC 
ACACACCACG ACCACTCCTA GCTTGTTACT CACTTGGTAG CTTGATCTGG 
TGAAAAAATC ACTCACCACG TCAATTATCA AATCAAGGTC AGGTAGGAAG 
AATGACAAGG TCTGCTTGGC GTATCGGACG ATAATTGTGT CGATTGTCCT 
GTGGAAGATG AAATGTATGT CTACTGTTGT

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wmc282(3c12) Plate reference : 3c12
WMC locus number : WMC282 Length of sequence : 405
Last updated : Lun 9 Nov 1998 at 17:25
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Bases 77-365 (complement) (ie. 3' side of microsatellite) match part of 9H6. (GT)12 85 to 108, (GT)8 111 to 126. Reverse primer is within the region matched to 9H6, but includes several mismatches. Bases 178 to 203 show homology to 21f1 bases 185 to 210 (26 in longest run, maxScore = 83), duplicate, 3c12 has priority.
PCR Comments : 51C anneal, product;
Forward primer details :
gTAgccAccTATATAAgTggATgT at 30 length 24 bases, Tm 57
Reverse primer details :
AAATAggTcggTAATcAgAAATgT at 208 length 22 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 202 bases.



Length of sequence : 405  Consensus sequence is :

..

CTTTGGTGTG TGCAAATGCC ATGGTTCTGG TAGCCACCTA TATAAGTGGA 
TGTTTAATTT GGTTATGCAA GCATGTCCTT ATAAGTGTGT GTGTGTGTGT 
GTGTGTGTAT GTGTGTGTGT GTGTGTTGTT GTTCTGTATG CAATCCCATA 
ACACAACACA CACATCTTAT TAGCAATAAT CGTCTGTGTT ATTATCGGTA 
TTCACACACA TTTCTGATTA CCGACCTATT TGCTGCGTAT CACACACATC 
TTGTTCATTT GAACCATTTC TGTTCTCATG TCTCAACGCA AACAGTTCAT 
CCGACTGAAC CGCATGCCGT ATATCGCACA CACCTTTGAT CTAGCTGACC 
ATTTTCATTG TGTTGCCTAA TCACAAACAG TTCATTCGGG TGAACCGTAT 
GTTGT

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wmc(3d12) Plate reference : 3d12
WMC locus number : WMC Length of sequence : 664
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Multiple inserts joined at 212-230 by linkers. No substantial microsatellite. Bases 224-344 (complement ) match part of 3e5.
PCR Comments :



Length of sequence : 664  Consensus sequence is :

..

TGGGTGCGTC CCCGAGACGT CGCGTTGTTT CCACACACGT CGTCATAAAT 
TACCCTCACA TTTCCCTCCT TACAGCTCTC TCCCCTCTTC CCCACTCGCA 
TCGGCGGCGG CGACGGCGGC GACCACCTGA GCTCTACTGC GAATCGATCC 
AAGACGGCGG CGGCAACCAC CGGAGCGCAT CGGCCATGGC AGTAAGACCT 
CGTCCTCTCT GTTAGTCCAC GCGTGGACTA ACCGGTCCGT GCACACCGAG 
GGCTTCGCCA TGCTCGACAT ATCGGAGCTA CGCGCCGTGG TGGTCGACAA 
CCAGACGTCG ACAGCGTGGG TGGAGTCCTG CGCGACGCTC GGCGAGCTCT 
ACTATGCCAT ATCGAAGGCG AGCAGCCTTC TGGGATTCCC AGCCGGCGTG 
TGCCCGACCG TCGGCGTCGG AGGCCATTTT AGCGGCGGTG GCTTCGGCAA 
GATGATGCGC AAGCACGGGG CTAGCCATTG ATAACATTAT CGATGCAGAG 
TTGGTGGACG TCAATGGCAC GCTCCTGAAC AAGACCACCA TGGGGGGACG 
ACGTCTTCTG GGCCATCAGA GGCGGCGGCG GCGGGAGCTT CGGCGTCGTG 
CTGAAGTGGC AGGTGAGGCT CGTGACCGTT CCCGGCGACG GTATCGGTGT 
TCAAGGTCTC GGCG

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wmc(3e12) Plate reference : 3e12
WMC locus number : WMC Length of sequence : 408
Last updated : Jeu 31 DŽc 1998 at 11:54
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Small interspersed microsatellites. Bases 156- 249 (complement) ie 3' of small microsatellite match part of 3e12. Bases 17-107 match part of 14e4. Bases 56 to 80 show homology to 14e4 bases 177 to 201 (25 in longest run, maxScore = 56). Bases 225 to 245 show homology to 17b11 bases 54 to 74 (21 in longest run, maxScore = 34). Bases 158 to 205 show homology to 19g3 bases 117 to 164 (48 in longest run, maxScore = 68). Bases 247 to 266 show homology to 2f2 bases 475 to 494 (complement) (20 in longest run, maxScore = 66). Bases 186 to 227 show homology to 25d10 bases 65 to 106 (42 in longest run, maxScore = 69). True duplicate with 2f2, 3e12 has priority. Near duplicate with 25d10, 3e12 has priority.
PCR Comments :



Length of sequence : 408  Consensus sequence is :

..

CACACACACA CCCCTACTTG CAAGTCGGTT ATCAACATGC AATTGGGGAC 
CCTCGACACA CACACACATC CCCTAGTTGC CAGTTGATGG TCAACTTGCA 
ACTAGGGGCG ACGAATAAAC ACACACACAC GCACCCACAC ATACACACAC 
ACACACCCCT AGTTGCGAGT CGATGGGCAA CTTGCAACTG GGGGTCCTCA 
ACAAACACGC ACCCCCTAGT TGTGAGTCGA TGCTTGACAT GCAACAGGGG 
ACCCCTTGAC AAAAAGGTAC ACACACACAC CTCTACTTGC AAGTCGGTTA 
TCGACATGCA ATTGGGGATC CTCGACACAC ACACATCCCC TAGTTGCAAG 
GTGATGGTCA ACATGCAACT GGGGGCCACA AATAAAACAC ACATACATAC 
ACGCACCC

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wmc(3f12) Plate reference : 3f12
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No sequence 24.6.97.
PCR Comments :
No sequence available for this clone
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wmc(3g12) Plate reference : 3g12
WMC locus number : WMC Length of sequence : 209
Last updated : Tue, Jul 29, 1997 at 9:41 am
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. No substantial microsatellite.
PCR Comments :



Length of sequence : 209  Consensus sequence is :

..

TGCTGGGCGT TTGCAACCGC CTCAACACTT CCAGATTTGT GAGAGGCCGC 
CTACGCATGA TTTGACGGTA TGCTAGCCCC CGGAGGCCAC CGGCCACAGT 
CGTAGACGTG CGAAGAGCAA TCCGCGTAGA GGGAAGTGTT CAGATACTTC 
TCCATCACAA AAAATTATGA AAACTTACCA TCAGTCCTAT AGCACATGTG 
CCCACGTTG

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wmc(3h12) Plate reference : 3h12
WMC locus number : WMC Length of sequence : 100
Last updated : Tue, Aug 26, 1997 at 5:48 pm
Owner : Keller, Zurich Date at Agrogene : 24.6.97 Public Domain : Yes
Comments : No trimming done to sequence. Matches central portion of second insert in 6D3. No substantial microsatellite.
PCR Comments :



Length of sequence : 100  Consensus sequence is :

..

TCGATCGATC TATCTCATTA GTTATGTCTC TCCCTTCCTC TGACAAACAA 
CAATTGATCC ACCGATCTAG TTAGGTTTGC TTACCAAACA CATGGTTCCC

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wmc(13A1) Plate reference : 13A1
WMC locus number : WMC Length of sequence : 292
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite. Bases 164 to 188 show homology to 25e9 bases 5 to 29 (complement) (25 in longest run, maxScore = 31).
PCR Comments :



Length of sequence : 292  Consensus sequence is :

..

GTACGAAACC ACCTCAAAGT TATTATTTCT GATCAATCCA TTGGGCTATT 
CCTATAAGTA TCACAGACAA CCCTAGAGTT CGTAGTAAAA TACACTACAA 
AAAAAAGACA CATCCGTGAC ATTTTGGGCC GAACAAATTT TTGTGTGTCA 
TACTTATGAC ACTTCTATGA CGATAATTGT GACAAAACCC GGTAGCATCA 
TAGATGTGGT TGGGCCCTAC TTCTATGACA AAAAATCATG ACAGAAAATG 
GGCTTCTCAT CCTGGGCAGG CCGGAGACGC AGCTGCATGA CA

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wmc(13b1) Plate reference : 13b1
WMC locus number : WMC Length of sequence : 209
Last updated : Wed, Aug 27, 1997 at 10:30 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Duplicate of 6D5. (GA)15 84 to 113, (GT)16 52 to 83.
PCR Comments :



Length of sequence : 209  Consensus sequence is :

..

GTAATTCAAC GGGTCCCACT TGTGCGTGTG CGCGCGTGTG TGTGTGCGTG 
CGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGACCCATCG GTCGATGCTC TAAATACATC TTTCAGCCAT 
ATGCATTGCT AATGATCAGT AAAAACTTAT ATAGCTGACA CAATCATATA 
GAACATCAT

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wmc(13c1) Plate reference : 13c1
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Jul 28, 1997 at 12:58 pm
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : No sequence 14.7.97.
PCR Comments :
No sequence available for this clone
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wmc283(13d1) Plate reference : 13d1
WMC locus number : WMC283 Length of sequence : 207
Last updated : Tue, Dec 9, 1997 at 11:15 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 20.10.9797 Public Domain : Yes
Comments : No sequence 14.7.97. Resequenced. Trimmed to insert at both ends. (CA)19 64 to 101, (CA)8 104 to 119.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cgTTggcTgggTTATATcATcT at 2 length 22 bases, Tm 62
Reverse primer details :
gAcccgcgTgTAAgTgATAggA at 131 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 151 bases.



Length of sequence : 207  Consensus sequence is :

..

ACGTTGGCTG GGTTATATCA TCTACACATT GTGACCGGGG CAACACACAC 
ACACAAACAC AAACACACAC ACACACACAC ACACACACAC ACACACACAC 
AAACACACAC ACACACACAT GTAAACACCT TCCTATCACT TACACGCGGG 
TCCCACTCTC CTTCCTTCTA GTTCGCGAGA ACATCATCAA TTGTAGGCAC 
AAGTGTG

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wmc284(13e1) Plate reference : 13e1
WMC locus number : WMC284 Length of sequence : 622
Last updated : Lun 30 oct 2000 at 16:59
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. (GT)8 552 to 567. Bases 81 to 90 show homology to 12d7 bases 32 to 41 (complement) (10 in longest run, maxScore = 29). Bases 98 to 114 show homology to TW7h08.1 bases 39 to 55 (complement) (17 in longest run, maxScore = 27).
PCR Comments : 51C anneal, smear.
Forward primer details :
gAAgggTggTgggAAcAAAT at 478 length 20 bases, Tm 63
Reverse primer details :
acAcAcTAAgAcTccATcTATc at 580 length 22 bases, Tm 52
Expected size of amplified fragment (Chinese Spring=) 124 bases.



Length of sequence : 622  Consensus sequence is :

..

ccttaggcgg tggactaccc cttcgcccct tagactatat atactccccc 
tccacctatg ttttagggtg agcattgtgt tagctcattt gtgagacaga 
gcttcgctca tccatatcgg atctactcct tgagagagac cgcggcccct 
cttcggagaa aatccacctt ggattcaaga cccctttacg ggaagatcct 
cttgtGGATT CAGGACCTCC TCATGGAGAA GAACCGACTA CCCTTTGTAT 
CATCCCTTGT TGGATTTGGA TCGTGTATCT CCTTGTGTTT CGAGGATCTA 
GCACATGTGT GATTGTTCTT GTTGGTTTGA GTGATTCTCT CGTGTTTCCC 
CTCGTGTTTC TCCTCGTGAT TCCCCTCGTG TCCTTCATGT TCTTCGCGGG 
ATCCGCTCCT TTCGTGAAAG ATCGGGCCAC TAGGGTTCTA CCCTACATCA 
TTGTTTATCA TACGGGTAAG GTTGGATGAA GGGTGGTGGG AACAAATGCT 
CCGCCTAGAG CATGTGTGTG TGAGATGGAG TCTTAGTGTG TGTGTGTGTG 
GGTGTGTGTG TGTGTGTGAG AGAGAGAGAG ATAGATGGAG TCTTAGTGTG 
TGTGTGTGTG CGTGTGTGTG TG

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wmc285(13f1.1) Plate reference : 13f1.1
WMC locus number : WMC285 Length of sequence : 372
Last updated : Mar 17 Nov 1998 at 10:49
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. Multiple inserts (2?) joined 371-380 by single linker - this is first insert . (CA)10 18 to 37, (CA)20 73 to 112.
PCR Comments : 61C anneal, product.
Forward primer details :
TgTggTTgTATTTgcggTATgg at 42 length 22 bases, Tm 64
Reverse primer details :
TTgTggTgcTgAgTTAgcTTgT at 309 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 289 bases.



Length of sequence : 372  Consensus sequence is :

..

TTAGGCCGTG GACTACCCAC ACACACACAC ACACACAAAG ATGTGGTTGT 
ATTTGCGGTA TGGAAAATGT TTCACACACA CACACACACA CACACACACA 
CACACACACA CATAATATCA GGCTTATTGG GCCATTTGCC ATCTAGAGTT 
GTCAGCTGTA ATTGGGCCTA GAAAGGGTGG ACGGTAGGTC ATCTTTGGCA 
AAGGTGCTCA AAATCAAACG AGTTACAGCA ATAGGGATTA ATTGATTAAT 
TACCTGTTGC TGCCTTGCCC TTGACAACGA CTCAGGCAGC GTAAGTGGCC 
AGATTAAAAC AAGCTAACTC AGCACCACAA CGCCGCTCCA GTGCTGCGGC 
ATTTCATCGA ACACTCTTGT AC

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wmc286(13f1.2) Plate reference : 13f1.2
WMC locus number : WMC286 Length of sequence : 413
Last updated : Ven 3 Nov 2000 at 10:39
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Multiple inserts were joined 371-380 by single linker - this is second insert. (CA)9 328 to 345, (GA)14 346 to 373. Weak homology with 1b11. Bases 276 to 304 show homology to TW6d02 bases 1 to 29 (29 in longest run, maxScore = 32). 13f1.2 is a duplicate of TW6d02. 13f1.2 has priority.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
cgggTTcTcTggATcggTTc at 211 length 20 bases, Tm 66
Reverse primer details :
ccccggccTTccTcATTcTT at 373 length 20 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 182 bases.



Length of sequence : 413  Consensus sequence is :

..

GACTAACCAG AAAAAGGAGT CTGCTAATAT CAAAAGGAGG CACCAAACAG 
CTATTGCATG AACATACATC TCTTTTGGAT CAGAAAGAGG AGGCAGTAGA 
AGAACTATGA TGAACTACGA AGAACACGCA GGAGCTTGGA AACCCTAATG 
AAGATCTGAG GCATGAAAGA AAGAAAGCTT ACGGTTGCAG GTCTACTGCG 
GAGGTCGCCG CGGGTTCTCT GGATCGGTTC AGGTCGATGC AGCGGCCAGA 
GTCGACGACG GAGATAAGGC GCGGCGGCGG CGAAGCTCGA GCAGTAGCAG 
GGTCACGAGA GGAAGAAGAC AGACAGACAC ACACACACAC ACACAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAAGAATGA GGAAGGCCGG GGCGAGCCTA 
TTTATAAGGA AAG

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wmc287(13g1.1) Plate reference : 13g1.1
WMC locus number : WMC287 Length of sequence : 203
Last updated : Mar 17 Nov 1998 at 10:51
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Multiple inserts (2) joined 203-221 by linkers - this is the first insert. Complex microsatellite (CT)4(AT)(CT)7(CA)7(CT)2. Bases 107-185 (ie. 3' side of microsatellite) have some homology to 6A1 (reverse primer has 2 mismatches to 6A1).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
AAATTccTAgcTcgTTgTccgT at 3 length 22 bases, Tm 63
Reverse primer details :
TTAgATgggTcTATcgAgTgcg at 150 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 169 bases.



Length of sequence : 203  Consensus sequence is :

..

TCAAATTCCT AGCTCGTTGT CCGTAACTCT CTCACGCACC ACTCTGCCGT 
CTCGGAGCTC TTCCCTGTCT CTCTCTATCT CTCTCTCTCT CTCACACACA 
CACACACTCT CCATCTCCCT GGGCCACACA TACACATGCA TATATCACTC 
GCACTCGATA GACCCATCTA AAACTCTCTC TCTCTCTCCC TCGTTATCTC 
TCC

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wmc(13g1.2) Plate reference : 13g1.2
WMC locus number : WMC Length of sequence : 483
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Multiple inserts (2) joined 203-221 by linkers - this is the second insert. Member of 1g1 family. (GA)10 196 to 215, (GT)13 295 to 320. Bases 406 to 475 show homology to 12h5.2 bases 493 to 562 (70 in longest run, maxScore = 105). Bases 346 to 388 show homology to 22g7 bases 182 to 224 (43 in longest run, maxScore = 82). Bases 356 to 382 show homology to 23a6 bases 1 to 27 (complement) (27 in longest run, maxScore = 65), true duplicate, 13g1.2 has priority. Bases 334 to 364 show homology to 25a8 bases 17 to 47 (complement) (31 in longest run, maxScore = 40).
PCR Comments :



Length of sequence : 483  Consensus sequence is :

..

GTACAGATGA TAAGTTTGCT AACTACTAAA GTAAATTGGA ATTTGTCCAT 
GTTATACAAG TAAATTAAGT TGATTGTTTA TCATACGGGT AAGGTTCGAT 
GAAGGGTGGT AGGAACAAAT GCTCCCGCCT AGAGCATGTG TGTGTGAGAT 
GGAATCTTAG TGTGTGTGTG TTTGTGTGTG TATGTGTGTG TGTGTGAGAG 
AGAGAGAGAG AGAGATGGAG TCTTAGTGTG TGTGTGTGTG ATGGAGTCTT 
AGTATGTGTG TGTGTGTGTG GGTGGGTGGT GGGTGCGTGC GTGCGTGTGT 
GTGTGTGTGT GTGTGTGTGT CGCGGGGTCC TCGGTGGCGG ATGTAATTTA 
AGTGTGCATG GCTTCATCAT AGAGAGGTGA TGTGTATGCC AGACGCCACC 
AATGATGGGG TGCGAGAGAG AAAATGCCCG TAGAGAGGGA AGAGAAGGTG 
TGTGCGCGTG AGAGGAGTCG ACATCGAGAG TAC

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wmc(13h1) Plate reference : 13h1
WMC locus number : WMC Length of sequence : 386
Last updated : Wed, Aug 27, 1997 at 10:35 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Duplicate of 9A1. (GT)8 327 to 342.
PCR Comments :



Length of sequence : 386  Consensus sequence is :

..

GTACTTAAGC TCGCTTAAGA GTAAACAACA ACCATGAACA ACAATCGTAA 
TTGAAACATG CAAGTTACAC CCATCAGGGT GGTCTTGTAT CGTCATAATA 
ACACATGAAC CTTCATTTTC CACTGCACGA ACTTTACACA AATAGTATGT 
GTGCACTCTT TTCATTGGAC TAGACACCGT TTATCATGAA CATTATGCCC 
ATCGGGGCAC CATCCTTTTT GAGCCGCTCC TTTGGAAGTC CATTGTTGCT 
CTCGAGAATT CGTATATTCC CGTCCTGCCC CTTCCGCCCT CTCTCTCTCT 
TTCTCTCTCT TCGTGTGTTT GTGTTCGTGT GTGTGTGTGT GTATGTGTTT 
TGTCGTATGG ATAGACGGAC CATAATTTAA TCTCTG

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wmc(13a2) Plate reference : 13a2
WMC locus number : WMC Length of sequence : 212
Last updated : Wed, Aug 27, 1997 at 10:36 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (CA)13 12 to 37. No room for primers.
PCR Comments :



Length of sequence : 212  Consensus sequence is :

..

GTACAACCAT TCACACACAC ACACACACAC ACACACATAT ATATATATAT 
AGACGGTCTG TTCTGCTAAT ATTAGCAGAA TAACTATTAT GCACACCACT 
TCGGCACTGC ACGCTCAACA AAAGTGCACT ACATCATTTT GACGACGAGC 
ACTGCAATAC AGACTAGTGA ACTTCGTGTC GAAAAAAATT GCACGCGGTG 
TTTTTTTGGT AC

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wmc(13b2) Plate reference : 13b2
WMC locus number : WMC Length of sequence : 400
Last updated : Tue, Dec 9, 1997 at 12:14 pm
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to insert at 3' end. No substantial microsatellite. Eyeballs say sequence contains a minisatellite-like motif.
PCR Comments :



Length of sequence : 400  Consensus sequence is :

..

CTCTGGTATG TATTTCGGAT GGGCCCGAGG CCTAGCCCAT GACATGGCCA 
AGGCCCAGAC GCTCAGCTTC GGCAGCCACG AGTCCATAAC GCACTTCCGT 
TCGCATGCAC GGACGCGAGT CAGGAGCGGA GCAACCGCGA CAGCCGCCTC 
CCCTCCTGTG CACCGCGGCG CCGCTGAGCC GCCGACGACT TTATCGCTCC 
TTGCCGCTTG TCTCCACGAC ATAGCCCAGG GCCGACGCTC TTCCTCAATC 
CCCTGCTCGG CTGCTCCCCG ATGCTCAGGA CTGTGGACTG CCGGTCTGCT 
GCTGCTCTAG CCGGCCGCCG CCGCCGCTCT ACTCCGGTCA TTGCCTGAAT 
CCGGACTCCC CCTCTCCGCG CCACCGCTCT ACTCCGCGCC ACCGCTGTAC

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wmc288(13c2) Plate reference : 13c2
WMC locus number : WMC288 Length of sequence : 383
Last updated : Mar 17 Nov 1998 at 10:51
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 20.10.97.97 Public Domain : Yes
Comments : No sequence 14.7.97. Resequenced. Trimmed to insert at both ends(CA)8 215 to 230, (CA)13 257 to 282, (CA)11 311 to 332, (CT)9 239 to 256. Bases 22-183 (complement) (ie. 5' side of microsatellite) match part of 5f10. Forward primer is within this conserved region. NB apparently internal Rsa1 site at 337-340.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
AAccAAAAcccTAATgTcAccT at 49 length 22 bases, Tm 60
Reverse primer details :
TAgAgcATgTgTggTgTgAgAT at 344 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 317 bases.



Length of sequence : 383  Consensus sequence is :

..

CAAATAGCCC TAGAGTTCAT AGTAAAATAA CACCTTAAGA CACACATCAA 
CCAAAACCCT AATGTCACCT AGATACTGTA ATGTCATCAA AAGTATCCGC 
GGGTATGATT ATACGATATG CATCACACAG TCTCAGATTC ATCTATTCAA 
ACGAACACAA AGAACTTCAA AGGCCCCCCG CCCACACAAA CACACACACA 
CACTAAGACT CCATCACACA CACACACACA GACTCCATCT CTCTCTCTCT 
CTCTCTCACA CACACACACA CACACACACA CACCCACACC CACACCCACA 
CCCACAAATA CACACACACA CACACACACA CACTAAGTAC TCCATCTCAC 
ACCACACATG CTCTAGGCGG AGCATTTTGT TCC

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wmc289(13d2) Plate reference : 13d2
WMC locus number : WMC289 Length of sequence : 360
Last updated : Mar 17 Nov 1998 at 10:51
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)13 221 to 246, (CT)10 178 to 197.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
cATATgcATgcTATgcTggcTA at 146 length 22 bases, Tm 63
Reverse primer details :
AgccTTTcAAATccATccAcTg at 324 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 200 bases.



Length of sequence : 360  Consensus sequence is :

..

GGCCAGGCAG GCCAAACACT CTCACTTGTC ACCCCAATGC AAGAGACAGG 
CAGGATGGGG GATGGATTTT ATTGGGAAGT GATGGTTGGT GCCTCCTCAT 
GCCAAGAAAG CTGCTAGCTT TTGGGACAAA AGAGAGGGCC ATGCCCATAT 
GCATGCTATG CTGGCTACCT AGGGTTTCTC TCTCTCTCTC TCTCTCTTCT 
CTCTCTACCT CTCTCTCACT CACACACACA CACACACACA CACACAAGGA 
CTAGAGAGAG AAGGAGAGCA CTGAAATGTG TGGCCACTCT GCTGGCTCCA 
CGATCTTGAA AAATCTCTTT TTGCAGTGGA TGGATTTGAA AGGCTATGTT 
TGTATTGTAC

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wmc290(13e2) Plate reference : 13e2
WMC locus number : WMC290 Length of sequence : 314
Last updated : Mar 17 Nov 1998 at 10:51
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 10-218 (ie. 5' side of microsatellite) match part of 5c7 - forward primer has 5 mismatches to 5C7. (GT)9 201 to 218, (GT)12 221 to 244.
PCR Comments : tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide (several loci), beware primer dimer.
Forward primer details :
gTgccATcATcAcTgcAAcTgT at 77 length 22 bases, Tm 65
Reverse primer details :
cTggTgcgAAgTcTggcTATcA at 272 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 217 bases.



Length of sequence : 314  Consensus sequence is :

..

GTTGCCTTAT TAATTAACAG AAAACAGGGT GAGAACTATC ACATGAGGCC 
GGTGAAAATA AGGCACACTG GGTTCAGTGC CATCATCACT GCAACTGTGC 
GTGCGATAGA AGTCTACATA TCGAGGGGGC TATGGAGAGA AAGACGTATC 
GAGTGTGTGT GAGCAAGGCA CAATGACACA ACTAGCATGT GTGTGTGCAT 
GTGTGTGTGT GTGTGTGTTT GTGTGTGTGT GTGTGTGTGT GTGTCTGTGT 
GTGTTTGAGA CAGAAACCAA TTGATAGCCA GACTTCGCAC CAGGGCCAGG 
GTTTTCGAGA GTAC

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wmc291(13f2) Plate reference : 13f2
WMC locus number : WMC291 Length of sequence : 441
Last updated : Mar 17 Nov 1998 at 10:50
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to insert at 3' end. (GT)26 174 to 225.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
TAccAcgggAAAggAAAcATcT at 26 length 22 bases, Tm 63
Reverse primer details :
cAcgTTgAAAcAcggTgAcTAT at 237 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 233 bases.



Length of sequence : 441  Consensus sequence is :

..

ATTCTCTACC GGCCAGCATA TTTGCTACCA CGGGAAAGGA AACATCTAGA 
TTCTAGAACA TCATTTACCT AGTTAATGTT TGACTAGGTA TTCATTCGGC 
CACTTCTTTC GTGTTTCAAA CAAGAGCCAT GGGATCAGCC TCTCTGTTTC 
AGCACATGGC CACTCCGTTT CTTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTTTTGG TGGGGGATAG TCACCGTGTT 
TCAACGTGGA CAACGTCGAA TCGTTTCTTA ACAATGGAAA ATAATGCTAT 
CAACTTACTG TTGCGATCAG ATCTGTGGTC CCACACTTCA GACGGATCGA 
CCTCCAACCC TAAACCAAAC AACTAGCCGG CACGGTAATT GGTCGTCTCG 
TCCCATAATA TTCTTCATCC AAGCAACAAC CGGAATAGTA C

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wmc(13g2) Plate reference : 13g2
WMC locus number : WMC Length of sequence : 171
Last updated : Tue, Jul 29, 1997 at 10:06 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 171  Consensus sequence is :

..

GTACTAAAGG TAGGTTCTAT ATAAGAAAAC ACTTCAAAAA AATCGTCAGT 
TCATTCTCCC TCTACTTCTT CTCCTCTGCC CCGTCGCCGC CGCCCCTCAT 
CGCCGTCCTC GTCACCATCG CCGCCGCCCC CGTCGCCGTC CCCATCGTCG 
TCTATCATCG TCATCGCCGC C

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wmc(13h2) Plate reference : 13h2
WMC locus number : WMC Length of sequence : 169
Last updated : Wed, Aug 27, 1997 at 10:40 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Duplicate of 11A4. (GT)12 64 to 87, (GT)9 138 to 155.
PCR Comments :



Length of sequence : 169  Consensus sequence is :

..

CGTGGACTAA CGTGCGCGTG TGTGTGTTGC TGCGTGTGTG TGCGCGTGCA 
TGTGTGCTGC TGCGTGTGTG TGTGTGTGTG TGTGTGTGCA CGCGCGTGTG 
CATGCGTGTG TGTGTGTGTT GGTGCGTGTG TGTGCGCGTG TGTGTGTGTG 
TGTGTTGCTG CATGTGTAC

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wmc(13a3) Plate reference : 13a3
WMC locus number : WMC Length of sequence : 230
Last updated : Wed, Aug 27, 1997 at 10:41 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (CT)8 161 to 176. No room for primers.
PCR Comments :



Length of sequence : 230  Consensus sequence is :

..

GTACGACGTC ACATGACATC ATCTTCATAA TTACCTAGCT CTGATACCAA 
ATGTTAATGT TGACTCACTC AACTCCTTAA GATGTGTTCT TCACACACTC 
TCAACGACAC CACACAACTC TAGCTCTGAA GCTTAATTGA ATCAGGCACC 
TCCTCCTCAT CTCTCTCTCT CTCTCTCACA CACACACACA CTCACTCACT 
CACACACACA CACACGGCTC GCACAAGTAC

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wmc(13b3) Plate reference : 13b3
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Jul 28, 1997 at 1:03 pm
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : No sequence 14.7.97
PCR Comments :
No sequence available for this clone
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wmc292(13c3) Plate reference : 13c3
WMC locus number : WMC292 Length of sequence : 243
Last updated : Mar 17 Nov 1998 at 10:10
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 96 -242 (complement) (ie. 3' side of microsatellite match part of 6d12. Reverse primer has 2 mismatches to 13c3. (GA)18 86 to 121, (GA)9 124 to 141.
PCR Comments : 61C anneal, product.
Forward primer details :
gAggATAcggcTggATcTgTgA at 51 length 22 bases, Tm 65
Reverse primer details :
TcTcgTccTcTccAgATcTAAA at 160 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 131 bases.



Length of sequence : 243  Consensus sequence is :

..

CACCGAGGAC GATCACGTGA ACGTCGCACC CTCTCGCCTT CCTCGGCGGA 
GAGGATACGG CTGGATCTGT GACCAGTGGT GACCAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGCGAGAGAG AGAGAGAGAG AGTGGCCACC 
ACTGTGGTGT TTAGATCTGG AGAGGACGAG ACAGTGTGAA GAAAGCAACA 
CTAGCAAGCA AGCGCGGAGG CTCCTGCCTA TATAGTGGAG TAC

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wmc293(13d3) Plate reference : 13d3
WMC locus number : WMC293 Length of sequence : 199
Last updated : Mar 17 Nov 1998 at 10:51
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Short (CGG)6 microsatellite 101-119. Weak homology to 11g4.
PCR Comments : 61C anneal, product.
Forward primer details :
cgTcTcTTTcATgTcAcTgccc at 63 length 22 bases, Tm 66
Reverse primer details :
AgTTTcgTcgcggTTcTcTggA at 171 length 22 bases, Tm 69
Expected size of amplified fragment (Chinese Spring=) 130 bases.



Length of sequence : 199  Consensus sequence is :

..

CTTATGAGAC TGGTCTTATA AATAGCCCGA CCCTCGGGTC TTTCTCATTC 
TCCTTCTTCC GTCGTCTCTT TCATGTCACT GCCCCCGTGC TGCTCGAGCT 
CCGCCGCCGC CGCCGCCGCA ACAGAGCTTC GCGTCGTCGT CGTCTTTGGC 
CGTTGCATCA CCCTGAACCG TCCAGAGAAC CGCGACGAAA CTCCTGCAT

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wmc(13e3) Plate reference : 13e3
WMC locus number : WMC Length of sequence : 247
Last updated : Tue, Jul 29, 1997 at 10:10 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end, and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 247  Consensus sequence is :

..

AAGCCAGATT TGGGCACGTA GACGGCCGAG CCCAGCCCAC CTTGATAACT 
ACTCTCGCTT GCCGTCGCCC GACAGGTAGA CAGCGGAGAC GAAATCAAAC 
TGGAGGACTC CGCCGCAATC GTCGCCGCCG CCGTCGCCGT CGCCATCTCC 
TCTGTTTCTA CTCGCCGTCC GTCAGGTATA CATACATATA CACCCTCCCT 
AGCTAGGTCA ACCGAATCCT GCCTTTTCTC CCTGTCCCTC TTTGTAC

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wmc(13f3) Plate reference : 13f3
WMC locus number : WMC Length of sequence : 207
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 6-143 (ie. 5' and 3' side of microsatellites) match part of 6A9. Bases 6-147 (ie 5' and 3' side of microsatellites) match part of 7B4. (CT)16 6 to 37, (CT)19 109 to 146. Too difficult to design primers, the microsatellites are containined within larger tandem repeats. Bases 127 to 171 show homology to 24G10 bases 8 to 52 (complement) (45 in longest run, maxScore = 24).
PCR Comments :



Length of sequence : 207  Consensus sequence is :

..

GTACCCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCCG ACAATCTTCA 
ATTCTGTCGC ACGCATCCAA CACAAAATTC TTGTTGGTCC CTCTCCCTTT 
CTCTCCATCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCGCC 
GACAATCTTC AATTCTATCG CACGCATCCA ACACAAAATA CTTGTTGGTC 
CCTCTCC

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wmc(13g3.1) Plate reference : 13g3.1
WMC locus number : WMC Length of sequence : 172
Last updated : Wed, Aug 27, 1997 at 10:43 am
Owner : Jager-Gussen, Saaten Union Date at Agrogene : 14.7.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Multi ple inserts (2) joined 173-190 by linkers - this is the first insert. (GA)8 156 to 171, (GT)10 136 to 155. No room for primers
PCR Comments :



Length of sequence : 172  Consensus sequence is :

..

GTACTAAGGT CTTGCAGTGG AAATTTTGGT GAGGTCAGAA GGGATAGAGA 
AAATGGAGTT CCTTTGCAGT TTCTAATAAG GTAAAGTATT GTTGGAATAT 
TTGTTCTAAA ACAATTTGGA TGAAAAGAAA ATAATGTGTG TGTGTGTGTG 
TGTGTGAGAG AGAGAGAGAG AG

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