Wheat microsatellite WMC 25/06/01

wmc(14a4) Plate reference : 14a4
WMC locus number : WMC Length of sequence : 340
Last updated : Wed, Oct 15, 1997 at 1:44 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 340  Consensus sequence is :

..

AAAAAATAAA AACGAGATGA ACAGGCTAAT GAACGAACTT CCGCTGTCTG 
CGACTAACCG ATGAACAGCG TTTGTTAAAA CGAACATACA AAAGAACGTC 
CGCTAACTAA CTAAACCGAA AAAACCGAAC CGATCTAATT AAAAAAACTA 
GAGCTAGGGT TTCTAAAAAA CCAAATGGTT TTTGAAAAAA ACCAAAACGG 
CGGCGGCGGC GGTTTCTACC TCCGGCGAGG CTCTGGCGGG GCGGCGGGGC 
TCCGGGTGGT GGCGTGGGTC GACGGATGGG CGGTACTTTA GGATATTCTT 
GACCGTTGTC CAGTGTTCCT TGCCGGGATT ACTTTGGTAC

Go back to index

wmc(14b4) Plate reference : 14b4
WMC locus number : WMC Length of sequence : 60
Last updated : Wed, Oct 15, 1997 at 1:44 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : No insert .
PCR Comments :



Length of sequence : 60  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC TCTAGAGTCG ACCTGCAGGC 
ATGCAAGCTT

Go back to index

wmc(14c4) Plate reference : 14c4
WMC locus number : WMC Length of sequence : 479
Last updated : Mar 10 Nov 1998 at 10:41
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 375-479 (complement) (ie. 3' side of microsatellite) match part of 8H9. Bases 418 to 449 (complement) show homology to 6g4 bases 184 to 215 (32 in longest run, maxScore = 77). Bases 356 to 414 show homology to 12d11 bases 1 to 59 (59 in longest run, maxScore = 95). (GT)11 276 to 297 plus several small microsatellites. Too difficult to design primers. Bases 346 to 378 show homology to 10g9 bases 70 to 102 (complement) (33 in longest run, maxScore = 68). Bases 381 to 443 show homology to 21e2 bases 1 to 63 (complement) (63 in longest run, maxScore = 79), duplicate, 14c4 has priority. Bases 369 to 416 show homology to 21h4 bases 200 to 247 (48 in longest run, maxScore = 89). Bases 348 to 389 show homology to 24a4 bases 118 to 159 (42 in longest run, maxScore = 63).
PCR Comments :



Length of sequence : 479  Consensus sequence is :

..

AGACATACCA GGTTGATGAG CGATCCACGA CTTTAGTTAA ACCACCAGAA 
GCACACCATC AGAACAACGA TAATGAATCA AATTAGCCAT CATGAAAACA 
TCCATGCAAA TATCAGGTAA ATGAATGCTT TGGGACTACA GGCATCAGCA 
TCGCAAAATG TAAAGAATGT AAATATACCT GTGCTATATG CCAACTCAAC 
CCATCAGTGT CCTTAGCAAC ATAATAAAGA GCAAATGTGA TATGTCCAGA 
TCAACATGTA TGTGTGCTCG GGGGAGTGTG TGTGTGTGTG TGTGTGTTGT 
GCTGCGTGTG TATATGTGTG TGTGTGTGTT TGTCGGATAT TGAGAGAAAA 
CAAACCTAAG GAGAGAAATG GGTATTCACG AAGAGATTGT GCGGGCCTTG 
AGGTGGGCAG GGGCCGGGTG AGGGTGTCAA AATGTGCGCG TTGATGCATG 
TGTTGCATGC GACCGTGCGA GGGACGGAC

Go back to index

wmc(14d4) Plate reference : 14d4
WMC locus number : WMC Length of sequence : 6
Last updated : Wed, Oct 15, 1997 at 1:44 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : No sequence provided 13.10.97.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(14e4) Plate reference : 14e4
WMC locus number : WMC Length of sequence : 613
Last updated : Mon, Mar 16, 1998 at 1:21 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 135-251 match part of 3e12. Bases 177 to 201 show homology to 3e12 bases 56 to 80 (25 in longest run, maxScore = 56). (CA)9 11 to 28, (CA)9 240 to 257, (CA)10 464 to 483, (CT)8 115 to 130, (CT)10 322 to 341. Part of sequence (eg 500-521) is reiterated (151 etc) twice. Minisatellite like (CCTAGTTGCGAGTTGGTGGTCA) repeated 4 times. Too difficult to design primers
PCR Comments :



Length of sequence : 613  Consensus sequence is :

..

CGACCCTCGA CACACACACA CACACACACG CATGCACGCG CGCGCGCGCA 
CACACACACA CATCCCCTAG TTGCGAGTTG GTGGTCAGCT TGCAACTGGG 
GGCCACGAAT AAAACTCTCT CTCTCTCTCT TTATCTAGTT GCAAGTCGGT 
TATCGACATG CAATTGGGGA CCCTCAACAC ACACACACAT CCCCTAGTTG 
CGAGTTGATG GTGGACTTGC AACTAGGGCC ACGAATAAAC ACACACACAC 
ACACACACCA CACCTAGTTG CGAGTTGGTG GTCAACTTGC AACTGGGGGG 
CATGAATAAA ACACACACAC ACTCTCTCTC TCTCTCTCTC TGTGTGTGTG 
TGTCTGGTTG TAATTCGGTT ATCGACATGC AGTTGGGGTC CCTTGACACA 
CACACACACA TCCCCCTAGT TGCGGGTTGA TGGTCAACTT GCAACTAGGG 
GCCATGCGAT AAACACACAC ACACACACAC ACACTAGTTG CGAGTCTGTT 
ATCAACATGC AATTGGGGAC CCTCGACACA CACACACACA GTCACACACA 
CCAGGTTAAT GATGAAATCC CTTAAGATAA AACAAACAAC AGAGGAACAA 
ACCAGCTGCG TAC

Go back to index

wmc317(14f4) Plate reference : 14f4
WMC locus number : WMC317 Length of sequence : 221
Last updated : Mar 17 Nov 1998 at 10:59
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)23 62 to 107.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Forward primer details :
TgcTAgcAATgcTccgggTAAc at 6 length 22 bases, Tm 67
Reverse primer details :
TcAcgAAAccTTTTccTccTcc at 123 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 139 bases.



Length of sequence : 221  Consensus sequence is :

..

GTACATGCTA GCAATGCTCC GGGTAACGCG CGACACTGAC GGTGTGGTCC 
ACGGCGAATT TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGGA CTGCAGCGGT GTGGAGGAGG AAAAGGTTTC GTGATGGTAG 
GAGGACTCTC GAGAGAAAAA CCAAAGCGGT CGGTGGACAT GCATCTTTTG 
TTTGGTTCGG TTGGCTGGCT C

Go back to index

wmc(14g4) Plate reference : 14g4
WMC locus number : WMC Length of sequence : 168
Last updated : Wed, Oct 15, 1997 at 1:44 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at 3' end. First 20 bases are probably corrupted linker. No substantial microsatellite.
PCR Comments :



Length of sequence : 168  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTTC GCCACCACGA CCGCCACCGA AAATACCTTC 
AATGCCGTCC TCCGCCTCGA CCGCTGCTGC CATCCCGCCG CAACTCTATC 
CGCCGTCGTA TCCGCCACCA GAGTCGCTGC CCACGGTATA CTATCTCCCC 
TCTCGTCCGC GGTTGTTG

Go back to index

wmc(14h4) Plate reference : 14h4
WMC locus number : WMC Length of sequence : 186
Last updated : Wed, Oct 15, 1997 at 1:44 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 186  Consensus sequence is :

..

GCCGCCTAGA AGAGGCCGTC GCCATTGGGG AGCAGAGGCG GCCATCACCA 
AAGTCGTGCG GTTTGGCCTG AACCTGAAGG CAGTGGCGCC AGCGAGCTGG 
GCAGAGGAGA GCTTCCTACA CGGCAATGAC TCTTTTGCCT TGGGATGTCT 
CCGCTCAGCA ACCCCCGGTT AACTGACCTC GGCACG

Go back to index

wmc(14a5) Plate reference : 14a5
WMC locus number : WMC Length of sequence : 6
Last updated : Wed, Oct 15, 1997 at 1:44 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : No sequence provided 13.10.97
PCR Comments :
No sequence available for this clone
Go back to index

wmc(14b5) Plate reference : 14b5
WMC locus number : WMC Length of sequence : 60
Last updated : Wed, Oct 15, 1997 at 1:44 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : No insert.
PCR Comments :



Length of sequence : 60  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC TCTAGAGTCG ACCTGCAGGC 
ATGCAAGCTT

Go back to index

wmc(14c5) Plate reference : 14c5
WMC locus number : WMC Length of sequence : 235
Last updated : Mon, Mar 16, 1998 at 1:24 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 1-132 (ie. 5' side of microsatellite) match part of 8A5. Bases 1 to 86 show homology to 8a5 bases 1 to 86 (86 in longest run, maxScore = 62). (CA)27 84 to 137, (CT)11 62 to 83. Too difficult to design primers.
PCR Comments :



Length of sequence : 235  Consensus sequence is :

..

GTACTGTAAA ATGGACCATC TTTGCTTTGC TTCCTTGCTC GATGCCGGCC 
ACTTCTTCCG TCTCTCTCTC TCTCTCTCTC TCTCACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACAATA ATTTGCTTCT 
TGTGCAGTGC CCGGCGAAGC ACGTGGCGTG CGGCCCCTGC GCCGAGAGCC 
AGGCCAACAA GTGCCCGGCG TGCGACGGCG CGTAC

Go back to index

wmc(14d5) Plate reference : 14d5
WMC locus number : WMC Length of sequence : 339
Last updated : Mon, Mar 16, 1998 at 1:25 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends.(GT)26 286 to 337. No room for primers.
PCR Comments :



Length of sequence : 339  Consensus sequence is :

..

GTACTATTGT TCTATGGTTT CGAGGCTTCA AAAAAAGAAC CCCTGAAAGG 
AAAACAGCAT GCCTGTACAT TTATCGGCTG CGATTTCCGT TGTGTGCGTG 
GTGAACTGAC GTGCAATGCG GAGTTCAAGC GGGTCTTATC CAAGCACGGA 
GATGAAGAAG CCATAGGATG AATGATGGGC AAAGACCAAA GAGTGCTGCT 
GTCACGCGGC TGGACCCGTG AAAGCAACCG GCATGTGTGC ACCCTGGCTC 
CTCGATAGCC CTTGGGGCGA TGCAAGGGCC AAATTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTAC

Go back to index

wmc318(14e5) Plate reference : 14e5
WMC locus number : WMC318 Length of sequence : 283
Last updated : Mar 17 Nov 1998 at 10:59
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at 5' ends and to Rsa1 at 3' end. Bases 57-149 match part of 1g5. Bases 84 to 97 show homology to 1G5 bases 89 to 102 (14 in longest run, maxScore = 27). Forward primer does not overlap this homology. (GT)11 115 to 136. Bases 80 to 93 show homology to 10d12 bases 359 to 372 (complement) (14 in longest run, maxScore = 28).
PCR Comments : 61C anneal, product.
Forward primer details :
cgTAAAATTAcggTgcATTgAT at 19 length 22 bases, Tm 60
Reverse primer details :
gTggAcTTTTgTggTTTTTgAg at 199 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 202 bases.



Length of sequence : 283  Consensus sequence is :

..

AGAAAAAAAA TTACGAAACG TAAAATTACG GTGCATTGAT GTTAAAATAG 
AGGGGGTGAA GAATAACTAT TCCTCACCGA GGGTGACGAA TAGTCAATCC 
CTATATATAT ATATGTGTGT GTGTGTGTGT GTGTGTGGAA GGGTGTGTGG 
TTGTGATGTT TTCAATGCCT AATGCACAAC CTTTGATGTG GCATTTGCCT 
CAAAAACCAC AAAAGTCCAC AAAGAGTGAG CTTCATGTGT GACACCTAGT 
ATAGGTTGGT CCCATCCCCA CTTTGACACG TAC

Go back to index

wmc(14f5.1) Plate reference : 14f5.1
WMC locus number : WMC Length of sequence : 299
Last updated : Lun 2 Nov 1998 at 9:56
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Trimmed to insert at both ends. Multiple inserts - this is first insert in this clone (ends Rsa1). Bases 1-143 match part of 5a2. Bases 81 to 125 show homology to 5a2 bases 411 to 455 (45 in longest run, maxScore = 83). No substantial microsatellite. Bases 251 to 267 show homology to 10f4 bases 132 to 148 (17 in longest run, maxScore = 27).
PCR Comments :



Length of sequence : 299  Consensus sequence is :

..

CACACGTTTT CGTCTTGAAT CTCTGGTAGA AACTTTGGGG CACTCTTTGA 
AGTTCTTTGT GTTGGTTGAA TAGATGAATA TGAGATTGTG TGATGCATAT 
CGTATAATCA TACCCACGGA TACTTGAGGT GACATTGGAG TATCTAGGTG 
ACATTAGGGT TTTGATCGAT TTGTGTCTTA AGGTGTTATT CTAGTATGAA 
CTCTATGATA GATCGAACGG AAAGAATATC TTCGTGTTAT TTTACTACGG 
ACTCTTGAAT AGATCGATCA GAAAGGATAA CTTCGAGGTG GTTTCGTAC

Go back to index

wmc(14f5.2) Plate reference : 14f5.2
WMC locus number : WMC Length of sequence : 342
Last updated : Mon, Dec 29, 1997 at 6:08 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Trimmed to insert at both ends. Multiple inserts - this is second insert in this clone. After sequence break matches part of 8c1.2 Bases 86 to 106 show homology to 8c1.2 bases 131 to 151 (21 in longest run, maxScore = 61). No substantial microsatellite.
PCR Comments :



Length of sequence : 342  Consensus sequence is :

..

GTACTTCGTT TGATGCTCCA ATGGTTAACC GAGGGACCGA AAAGGCCTCG 
TGTGTGTTGC A
(break)
CG TATCGATCAC GATCGCGTGG CTGAAAACCG 
CACCTCATTT GGATTTTCCT AGCTCCCTCT ACCTATAAAT ATGTGGCCTT 
CCCGAAGATT CCGCGCAGAT CAAACCCTAA CTTTTCCCCT CGCGCCGCCG 
GACACTTCTT CCACCGACGG ACATGCCCAG TCGCCACCTC AATTCATCCA 
ATCAGAAGCT GCCACGTCAC CGCCTTCGCC GCCTCAGCCA ACCGCGAATC 
GCCACCTGTC CACCGCCGCC GTCCCCGCTC CGCCGGGCTC GTGGAGCCC

Go back to index

wmc319(14g5.1) Plate reference : 14g5.1
WMC locus number : WMC319 Length of sequence : 361
Last updated : Mar 17 Nov 1998 at 10:59
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Multiple inserts in this clone - this is first insert. Bases 195 to 220 show homology to 19b4 bases 280 to 305 (26 in longest run, maxScore = 68). Forward primer has 4 mismatches to 19B4, reverse primer is outside this region (CA)15 191 to 220.
PCR Comments : 61C anneal, product.
Forward primer details :
cgAgcgcTAcAggTAggTATcc at 53 length 22 bases, Tm 64
Reverse primer details :
gAggTATcATcggcTTgAggTg at 246 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 215 bases.



Length of sequence : 361  Consensus sequence is :

..

GTACCTCTAT AGTTAATTGG GCCTCCGTCT TCCCCCACCA CACACCGATT 
GTCGAGCGCT ACAGGTAGGT ATCCATGACA TAGAGACAAA CTACACATGA 
CCCCTGTCAC GTTCCAATAG GCCAGCCACT CTATATATTT GACATGGGCA 
CACACAAATT CGATGGCACT CTTTATCGAT CGCGCGTGCG CACACACACA 
CACACACACA CACACACACA TCATCCCTCT CTTCGATTCT ATGGACACCT 
CAAGCCGATG ATACCTCCAC TCTCTTCTCA TGGATCGCCC CCCTCTATAT 
ATGTTATATC CAGGACTCTA TTTCACACAC ATTGCATATG TTACATTTTT 
TGATTGACCC C

Go back to index

wmc(14g5.2) Plate reference : 14g5.2
WMC locus number : WMC Length of sequence : 322
Last updated : Lun 9 Nov 1998 at 14:46
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Multiple inserts in this clone - this is second insert. Bases 14 to 143 show homology to 17f7 bases 319 to 448 (130 in longest run, maxScore = 89). (GT)18 56 to 91. Too difficult to design primers. Bases 55 to 154 show homology to 23b1 bases 1 to 100 (complement) (100 in longest run, maxScore = 43), duplicate with 23b1, 14g5.2 has priority.
PCR Comments :



Length of sequence : 322  Consensus sequence is :

..

GGCTTTACTC TGCGTGTGTG TGTGTGGCTT GATGGTGAAT ATGTGTGGCT 
TGACTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGGCTTGATT 
GTGTGTGTGG CTTGACTGTG TGTGGCTTGA TGGTGAATTT GTGTGTGGCT 
TGACTGTGTG TGTGTGACTC TGTGAATATT GCATTTGCTT GATGGTGTGG 
CTGGTATTCT TTGTTGAATG ATTGGTTGTA TTACTTGTGT TTTATATTGT 
TGTGGCACGA TTGATTGTTG TTTGTTGAAT GCGAACTCGT ATCTTGACGA 
ATAATCATGT ATGCTAACTT GG

Go back to index

wmc(14h5) Plate reference : 14h5
WMC locus number : WMC Length of sequence : 76
Last updated : Wed, Oct 15, 1997 at 1:46 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 76  Consensus sequence is :

..

GATGGCGGAG AAGGCCGGCA CCGTGGCGGC CGGACGGCCC AAGCCGCCGT 
CGGTCAGATA CGGGCAGAAC GAGTAC

Go back to index

wmc320(14a6) Plate reference : 14a6
WMC locus number : WMC320 Length of sequence : 396
Last updated : Mar 17 Nov 1998 at 10:59
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)12 24 to 47.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
cATgcTTTcAAgAccTAcgAcA at 4 length 22 bases, Tm 62
Reverse primer details :
gcAAAcTTgcTcTgcTTgAcTc at 78 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 96 bases.



Length of sequence : 396  Consensus sequence is :

..

GTACATGCTT TCAAGACCTA CGACACACAC ACACACACAC ACACACACGA 
AAAGGCCTGC GCCTTGAGGC CAAAACAGAG TCAAGCAGAG CAAGTTTGCA 
TCAACAAGAG AGAAACATCA GCAACCAGGC CACGGCGAAT CAGCAAGGAC 
GAATTCCAAT CTAGGCCAAT AATGCTAGAC GGGGCGGCGG CGGCGAGGAA 
GCGGATCGAG GCTCTCACCG TGGCCCAACA GCTTGCTGCA GATTGGTAGC 
ACCTGCGTGA ACGGGACAAG CTTCTGGTGC TCCTGCAACA GCTCCGTCAA 
GTAGTGGCTG CGGCGGCACC GACGCCATCC AATCCGGCGG GCACGGAAAA 
ACAGAGGCAA CCAATTCAGC GAGGCCGTCC ACCCAGCAAA ACGCAC

Go back to index

wmc(14b6) Plate reference : 14b6
WMC locus number : WMC Length of sequence : 173
Last updated : Mon, Mar 16, 1998 at 1:34 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Duplicate 4b10. Bases 135 to 155 show homology to 4b10 bases 135 to 155 (21 in longest run, maxScore = 67). (CA)14 75 to 102.
PCR Comments :



Length of sequence : 173  Consensus sequence is :

..

GTACACTATA CACCATCTCT CTGTAGGAGT AGGTCGATGT GTCGCTCTCT 
CTAACACACA CATGTTGTTA AAAACACACA CACACACACA CACACACACA 
CAGGTTAATA GAATAGGAAA ATCGTGTGAG TGCGAAGCCA CAGGAAGTGA 
GATACAAATG GAGTTAGTCC ACG

Go back to index

wmc(14c6) Plate reference : 14c6
WMC locus number : WMC Length of sequence : 351
Last updated : Mon, Dec 29, 1997 at 6:08 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 141-310 (complement) match (weakly) part of 1F5. Bases 220 to 232 (complement) show homology to 1F5 bases 304 to 316 (13 in longest run, maxScore = 32). No substantial microsatellite.
PCR Comments :



Length of sequence : 351  Consensus sequence is :

..

CTGTGAAAAC AAAAGAAACA GAAATCTCCT GGTTCCTATG CGGCACTGTA 
GCTGCGGCTG TAGCAGTCTG CCACATGGCA GTTTGTGATT GGCCAAAGGC 
TCCTGTGTGC GTGCTCTCAC CAGAAGCAGA GGAAGCGGCA ACGGTGGCTC 
GGGCATCGGC GGTGGCGCTC CTCCGGCGAC TCTTCGGTGA GGGGGGAGTG 
ACAGGAGGAT GCGGCGGTGA GTGGCGGAGG TGGTGGTGGT GTTTAACGAC 
AATTGGGCTC ACTCTGAAGG AGACGGCGAC CATGTGAACA TGGCATGCTC 
CAGTGGCGGC GGATGTCAGT GGCGGCGGCG TTCTGGTGCT CTACAGGGTA 
C

Go back to index

wmc(14d6) Plate reference : 14d6
WMC locus number : WMC Length of sequence : 144
Last updated : Wed, Oct 15, 1997 at 1:46 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 144  Consensus sequence is :

..

GTACGACGCG AGAGGGGAAG GGAGGAAGGG AGAAGCTCAG CGAGCGGCTA 
CGAAAAGCCT GTGCGAGCTT AGGAGCAACA GAGGTCGACA GAGACGGTGG 
CGGGCGGCGG CGGCGGGGAA GAAGTGGTCG GGTCGGTGCT GTGG

Go back to index

wmc321(14e6) Plate reference : 14e6
WMC locus number : WMC321 Length of sequence : 375
Last updated : Lun 30 oct 2000 at 16:59
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 57-191 complement match part of 13H3. Bases 148 to 180 (complement) show homology to 13h3 bases 13 to 45 (33 in longest run, maxScore = 62). (GA)9 286 to 303, (GA)8 314 to 329. Bases 228 to 258 show homology to TW4f01.1 bases 102 to 132 (31 in longest run, maxScore = 40). Bases 238 to 261 show homology to TW2h01.1 bases 10 to 33 (24 in longest run, maxScore = 23). Bases 104 to 137 show homology to TW2a02 bases 69 to 102 (34 in longest run, maxScore = 78). Bases 169 to 206 show homology to TW6h05 bases 300 to 337 (38 in longest run, maxScore = 95).
PCR Comments : 61C anneal, product.
Forward primer details :
gggAgcAgAcTATTTggAAgAg at 230 length 22 bases, Tm 61
Reverse primer details :
cgccATTTATggAcAcAAA at 357 length 19 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 146 bases.



Length of sequence : 375  Consensus sequence is :

..

TACATAATTA GAGAGTGAGT GTGTGAGATA CACATGCGAA CATGGGGAGA 
GATGAGGATC TAGATTAATG GGTGTTTGTG CGTGTCTGTG TGTGTTTGTG 
TGTGCGTTTG TGTGTGAGAG GGAGTGTGTG TATGTGGAGT AGAGAGTCCA 
CTAATGATGT AAACCTAGTA TAAGGGAAGG GGGAATGGTG TGACATGTGT 
AGTAGCAGGA TAGCACGGGG GCGGGCGGGG GGAGCAGACT ATTTGGAAGA 
GACATCGAGT GAGTGTGGGA GAGGGGTGTG AGAGCGAGAG AGAGAGAGAG 
AGAGCTAGCG ACGGAGAGAG AGAGAGAGAG TAAGAGAGGG GGCGAGAGAG 
GGGGCGTTTG TGTCCATAAA TGGCG

Go back to index

wmc(14f6) Plate reference : 14f6
WMC locus number : WMC Length of sequence : 282
Last updated : Mon, Mar 16, 1998 at 1:37 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Duplicate of 9G1. Bases 1 to 251 show homology to 9g1 bases 60 to 310 (251 in longest run, maxScore = 183). (GT)8 266 to 281. No room for primers.
PCR Comments :



Length of sequence : 282  Consensus sequence is :

..

GGCCCAACAA CACCGTTGGT GATAAAGCTG TCTCTTTAGT TCGCCATCGG 
CCTGCTTCCC CCGTCATGTT TCCACCGTCG TCGCACGAGG CCCCGCGACT 
CCATGCGCCT CCCCGAGCTT GCTTCCCAAG ACCGGGTTTT GCCGACGCGG 
CCTCTGCAGT TTTCTGAAAA AAAAATTGTG TGGTTTGTGT GAGTGTTTGT 
GTTGTTGGTG TGTGTGGGTG TGATTGTTTG TGTGATGGTG TGTGTGTGTG 
TCAGTAAGTG TGATGGTGTG TGTGTGTGTG TG

Go back to index

wmc(14g6) Plate reference : 14g6
WMC locus number : WMC Length of sequence : 284
Last updated : Wed, Oct 15, 1997 at 1:46 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 284  Consensus sequence is :

..

GTACCGCGCG AAGACGTCGG CGCTGAGCAC GCCTGCAGAA CGCGACCAAA 
ATCGAAACCA TCAGAGAAGA TAAAACCGGG GGCGAACAGC GAGAGCTCGA 
GGAAACAAAA TTGGGGGAGA TAGCACAGTA GAAGGGATGC TCACAGCCGA 
TGATGTTCCC CAGGTGCGGG ACGTTGTTGA CATAGGGCAA AGCGCTGGTG 
ATGAGTATGT TGCGGCGACC GGGGATCGGA AGCTTCGGCG GCGGCGGCGG 
TGACGCCATG GGGAGGGACG AGAGGGTTTA GCCG

Go back to index

wmc(14h6) Plate reference : 14h6
WMC locus number : WMC Length of sequence : 167
Last updated : Mon, Mar 16, 1998 at 1:38 pm
Owner : Hjortsholm, Sejet Date at Agrogene : 13.10.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)15 117 to 146. Too difficult to design primers.
PCR Comments :



Length of sequence : 167  Consensus sequence is :

..

GTACTGAAAA AACTCATTCT TTATTTATAT TGGTGTTGTA TGAAAGACAA 
AAAAACAGAA AAAACTAAAA GCTAAAAACA GAAAAATTGT GTGTGTGCGC 
GTCTAGTGTG TGTGTAGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGCAT 
GTAGTGTGTG TGCTCTT

Go back to index

wmc(17A7) Plate reference : 17A7
WMC locus number : WMC Length of sequence : 417
Last updated : Mon, Dec 8, 1997 at 6:07 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 417  Consensus sequence is :

..

GTCTCGTCCT GCCGAGCAAG CGCGTCTGCT GCTTCCTACT GGGTAGCGTC 
GGAAAGCGGA CGGCGGCGAC GATTGATCTC GTCCTGAACG GAGCAGGGGC 
GGCGGCGGCG AAGGATAATC CGGACGGAGA CGGTGAGCTC ACTTGTCTCT 
GCGCCTAGCT GCCGTGGTCA AGCAAATTTG AGTAAGCATA TACTATACTA 
AGCATCCGTA TGCCTTCTGT AGTTCTGTGG CAACAGAGAG CACTCGACAG 
CTTACAAATG CATGGCATTG CCATTTGCCA GTCAATAGCA AGGTTCACAC 
ATGCACACCA TCTGTCTGGT TCAATACCCC AGAGCTGGAA TGCGCAGGCA 
AGCAACTCAT CTTATCATAG TACAGTGTTG AAACCTTTAC TATCCTTGTT 
ATTCACACAA GTTCGAG

Go back to index

wmc322(17b7) Plate reference : 17b7
WMC locus number : WMC322 Length of sequence : 123
Last updated : Mar 17 Nov 1998 at 10:59
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)24 40 to 87. Forward primer reduced size to lower Tm.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
cgccccAcTATgcTTTg at 12 length 17 bases, Tm 61
Reverse primer details :
cccAgTccAgcTAgccTcc at 88 length 19 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 95 bases.



Length of sequence : 123  Consensus sequence is :

..

CAATAGCACG CCGCCCCACT ATGCTTTGGC CGAGGGTCTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGGA GGCTAGCTGG 
ACTGGGATCA TGCTATAAAT GCC

Go back to index

wmc(17c7) Plate reference : 17c7
WMC locus number : WMC Length of sequence : 39
Last updated : Mon, Dec 8, 1997 at 6:08 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 39  Consensus sequence is :

..

TCCGTGGGAG CGTTACCCTG ACCAATCAGA ACACATAAT

Go back to index

wmc(17d7) Plate reference : 17d7
WMC locus number : WMC Length of sequence : 234
Last updated : Mon, Dec 8, 1997 at 6:08 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 234  Consensus sequence is :

..

GGCATGGGCA GACCGCTAGG GATTGCCGGC GGCGGCGGCG GCGAGCTAGC 
GTTCAAGGGG GGAGTGATAC ATCCATTTTG CATCATGCTT TTATATTGAT 
ATTTATTGCA TTATGGGCTG TTATTACACA TTATGTCACA ATACTTATGC 
CTATTCTCTC TTATTTTACA AGGTTTATAT ACAGAGGGAG AATGCCGGCA 
GCTGGAATTC TGGGCTGGAA AGGGAGCAAA TATT

Go back to index

wmc323(17e7) Plate reference : 17e7
WMC locus number : WMC323 Length of sequence : 359
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 273 to 293 show homology to 9h4 bases 81 to 101 (21 in longest run, maxScore = 21) = mostly tetranucleotide repeat. (CA)11 253 to 274, (CA)11 297 to 318, (CT)11 231 to 252.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
AcATgATTgTggAggATgAggg at 180 length 22 bases, Tm 65
Reverse primer details :
TcAAgAggcAgAcATgTgTTcg at 319 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 161 bases.



Length of sequence : 359  Consensus sequence is :

..

CCAACTCCCA AGGTAAAAAA ATGATGTCTC TTTGCACAAA TGCAAGAAGG 
TGCTAGGAAG GATGTGGAGA GGGCATTTGG AGTGATCCAA GCTCAGTGGG 
GTATTGTTTG TGGAGTTGCA ATGATGTGCG ACAGGAGACA TTGTGCCAGT 
TGTTGACATG TGTTGTGTAG TCTTGCACAA CATGATTGTG GAGGATGAGG 
GCTAAGGGGC AACACACTCA CACACACACA CTCTCTCTCT CTCTCTCTCT 
CTCACACACA CACACACACA CACACGCACG CACGCACGCA CGCGCGCACA 
CACACACACA CACACACACG AACACATGTC TGCCTCTTGA AACAAGATGC 
AGAGCATGT

Go back to index

wmc(17f7) Plate reference : 17f7
WMC locus number : WMC Length of sequence : 482
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 319 to 448 show homology to 14g5.2 bases 14 to 143 (130 in longest run, maxScore = 89) (zone is 306-482). (GT)18 361 to 396. Too difficult to design primers. Bases 326 to 347 show homology to 21c9 bases 2 to 23 (complement) (22 in longest run, maxScore = 24).
PCR Comments :



Length of sequence : 482  Consensus sequence is :

..

GATGACGTGC TGCTAGTTAG TCCCCTTAGA CTTTGTTCTT GATAAAAGAG 
CATAACTCCA GAATTTTGTC ATTACACACC TGGGCATATA ATTCTGTTGT 
GAGCATAACC CCAGAATTTA TTTATCTGTG AAAGCTTGTT CATCACATGT 
TTTTTGTCAA TGCTGCTAAC TTTATAGCTT TCTGAGATTA CTTCGGTCTT 
GCATGTATTA GCCTTCTGTT TGTGCCCCTA TCTAAGTCTG TTTCTTGTGT 
GCAAGCGCTC CATTCTGTGT GTGTCACTGT GTGTGGCTTG ATGGTGAATA 
TGTGTGGCTT TACTCTGTGT GTGTGTGTGT GGCTTGATGG TGAATATGTG 
TGGCTTGACT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGGCT 
TGATTGTGTG TGTGGCTTGA CTGTGTGTGG CTTGATGGTG AATTTGTGCG 
TGGCTTGACT GTGTGTGTGT GACTCTGTGA AT

Go back to index

wmc324(17g7) Plate reference : 17g7
WMC locus number : WMC324 Length of sequence : 338
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 27 to 61 (complement) show homology to 6e4 bases 28 to 62 (35 in longest run, maxScore = 45), zone is 1-80. Primers do not overlap the region. (CA)13 106 to 131, (CA)14 134 to 161.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
AAcTAgggggcAcggcAA at 86 length 18 bases, Tm 66
Reverse primer details :
gATgTgATcgcAgTcggc at 231 length 18 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 163 bases.



Length of sequence : 338  Consensus sequence is :

..

CTAGTTGCAA GTTGATGGTT GACATGCAAC TGGGGACCCT TGACAAACAC 
ACACATCCTT AGTTGCGAGT CGATGGGCGG CTTGCAACTA GGGGGCACGG 
CAAAACACAC ACACACACAC ACACACACAC ACGCACACAC ACACACACAC 
ACACACACAC ACCCTGGTTG CGAGTCGATG GTCAACTTGC AACTCGGGAT 
TAATCCCGAA GGTAGATGTA GAGGTAGCGT GCCGACTGCG ATCACATCGA 
CTTTGGAACC GTTTCCCACG CGCATCGTCA CCTCGTCCTT TGCCAGTGCC 
CGCTTATTCT GTAGTCCCTG TTTCGAGTTG CAAATATT

Go back to index

wmc325(17h7) Plate reference : 17h7
WMC locus number : WMC325 Length of sequence : 488
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 417 to 457 (complement) show homology to 13c5 bases 249 to 289 (41 in longest run, maxScore = 82) zone is 227-488, primers apparently ouside this homology. (CA)15 60 to 89, (CT)13 34 to 59. Bases 131 to 151 show homology to 24d6 bases 144 to 164 (21 in longest run, maxScore = 38).
PCR Comments : 61C anneal, product.
Forward primer details :
gcAgAgcAccTTTTgTTTTgAg at 4 length 22 bases, Tm 63
Reverse primer details :
gccggAAAAAATgAAgcgAgAA at 94 length 22 bases, Tm 68
Expected size of amplified fragment (Chinese Spring=) 112 bases.



Length of sequence : 488  Consensus sequence is :

..

CCAGCAGAGC ACCTTTTGTT TTGAGATTTC GTTCTCTCTC TCTCTCTCTC 
TCTCTCTCTC ACACACACAC ACACACACAC ACACACACAT TGTTTCTCGC 
TTCATTTTTT CCGGCACTTG AATGCACACT GTTTGTTTAT CTTCGTGATG 
CTTTAAGTAT GCCTCACACA AATGATATCT ACCTATCCCT TAATATAGCT 
AGATATGTGT CACACAAAGT CCTTCTCCCT ACTATCAAGA TGCGCATGCG 
TTGCACGGAA CATCAAGATG CATTTGTATG AGTAGTTTAT CTTGTGGGAG 
AAAAGGACGA ACAAGGGAAT GCCGTATTTG CAAATGCGGA GAGGGGTTGG 
GTATCTTTTT GCAAAATTGC CATAGTTTCC TTCCTAACCG TCGAATATAA 
ATCGGATGGC CTATAATGCA GGATGGCAGG AACACCATCA TCACCAACTC 
TGTTTTTATA AGAGTAGAGA TATGTAAGTG TCACTACC

Go back to index

wmc(17a8) Plate reference : 17a8
WMC locus number : WMC Length of sequence : 456
Last updated : Lun 30 oct 2000 at 16:59
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Trimmed to insert at both ends. Bases 353 to 438 (complement) show homology to 6c10 bases 277 to 362 (86 in longest run, maxScore = 60). (GA)8 31 to 46, (GA)15 189 to 218, (GA)31 251 to 312. No room for primers. Bases 400 to 440 show homology to TW1c07 bases 73 to 113 (41 in longest run, maxScore = 56). Bases 376 to 431 show homology to TW2b10 bases 84 to 139 (complement) (56 in longest run, maxScore = 51). Bases 125 to 169 show homology to TW2c10 bases 57 to 101 (45 in longest run, maxScore = 46). Bases 340 to 449 show homology to TW6e12.1 bases 1 to 110 (110 in longest run, maxScore = 64). 17 a 8 is a duplicate of TW6e12.1. 17a8 has priority.
PCR Comments :



Length of sequence : 456  Consensus sequence is :

..

GAGGGAGAGA GAGAGGGAGA GAGAGAGAGG GAGAGAGAGA GAGAGAGGGA 
GAGAGAGAGG GAGAGAGAGG GAGAGGGAGA GAGAGGGAGA GAGAGAGAGA 
TGGAGAGAGA GAGATGGAGA GAAAGGGAGA GGGACCAACA AGAATTTTGT 
GTTGGATGCG AAGGGAAAGC GAGAGGGAGA GGGAGAGGGA GAGAGAGAGA 
GAGAGAGAGA GAGAGAGATG GAGA
(break)
ACAGAATTG AAGATTGTCG 
GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
GAGAGAGAGA GAGGGAGAGA GAGAGAGGGA GAGAGAGGGA GAGGGAGAGA 
GAGAGGGAGA GAGAGAGAGA TGGAGAGAAA GGGAGAGGGA CCAACAAGAA 
TTTTGTGTTG GATGCGTGCG ACAGAATTGA AGATTGTCGG TGAGAGAGAG 
AGAGAG

Go back to index

wmc(17b8) Plate reference : 17b8
WMC locus number : WMC Length of sequence : 494
Last updated : Mon, Dec 8, 1997 at 6:09 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 494  Consensus sequence is :

..

GGGATCTTGT TCCTCCGCTC CGTATTTAGG ATTTGGTTTT GTAGGTCGTG 
GTGTGTCATT GGAATGGTGG AATCGGCGTC CGAACGAACA AATCTGCTTC 
AACTTTACTC CCACACCGGT GGTGCCCTTC ATGGCGGCGT CTCAAACTTG 
ATGGCATCGT GTGTTTGCCA ATCCTTCAGA TCGGCGGCAT TAGGATTCAT 
GGATGGTGTT GGCGAGGCGG CGGCGGCGAC TCCATCAGGT GTGTCTCTCA 
TTCTGCTATG TCCCCATTGC GTGGCGTTGT CTCCGATGTC ATGATGGAGC 
AGGGAGGTTT TGTCGTTCAA GAGTTCGCGC AGACGGCTGT CTACACAAGT 
GACAGTTGGA AGATGGTGCA TCTTGTGTTG GGTTTGCGGT TGAAGGCTAA 
CAGTTCTGGT TTCCTTCTCC GACGTCGAGC ACTCGGTGCG TTCGGTTTAT 
TCGGTTTATA CGGTTTTTCA GTTTATACGG TATTAATACT TCGG

Go back to index

wmc(17c8) Plate reference : 17c8
WMC locus number : WMC Length of sequence : 151
Last updated : Wed, Mar 18, 1998 at 10:18 am
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 101 to 120 show homology to 4f3 bases 251 to 270 (20 in longest run, maxScore = 43). (GT)13 1 to 26. No room for primers.
PCR Comments :



Length of sequence : 151  Consensus sequence is :

..

GTGTGTGTGT GTGTGTGTGT GTGTGTTGGT GCAGCATGTA CTTACCATGA 
CGGCGTCAAG CTCAGCCAAA GACGAAGGCC CACCTAGCGT GCCAGAGGCT 
GAGGACGGGC TGCTATGAGC GGGAGCGACT GCGAGAGGAG GCCCGATTGC 
G

Go back to index

wmc(17d8.1) Plate reference : 17d8.1
WMC locus number : WMC Length of sequence : 147
Last updated : Mon, Mar 16, 1998 at 1:51 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multiple inserts (2) in this clone, this is the first insert. Trimmed to insert at both ends. Bases 74 to 105 show homology to 3d2 bases 78 to 109 (32 in longest run, maxScore = 50). (GA)9 18 to 35, (GA)9 60 to 77, (GA)8 131 to 146. Near duplicate of 3d2.
PCR Comments :



Length of sequence : 147  Consensus sequence is :

..

AGAACTGAAG ATTGTCAGAG AGAGAGAGAG AGAGACAGAG AGACAGAGAG 
AGAGAGACAG AGAGAGAGAG AGAGAGAGGG ACCAACAAGG TTTGTGTGTT 
GGATGTGTGT GACAGAACTG AAGATTGTCA GAGAGAGAGA GAGAGAG

Go back to index

wmc(17d8.2) Plate reference : 17d8.2
WMC locus number : WMC Length of sequence : 664
Last updated : Lun 9 oct 2000 at 14:43
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multiple inserts (2) in this clone. This is the second insert. Trimmed to insert at both ends. Bases 1 to 105 show homology to 4h4 bases 181 to 285 (105 in longest run, maxScore = 210). Bases 264 to 510 show homology to 19d6 bases 514 to 760 (247 in longest run, maxScore = 204), ie exact match to sequence after the break. (GA)9 542 to 559, (GA)9 584 to 601, (GA)8 655 to 670. No room for primers. Bases 353 to 512 show homology to 10d7 bases 187 to 346 (complement) (160 in longest run, maxScore = 135). Bases 476 to 512 show homology to 22f2 bases 81 to 117 (complement) (37 in longest run, maxScore = 31). Bases 275 to 448 show homology to 10h3 bases 246 to 419 (complement) (174 in longest run, maxScore = 132). Bases 495 to 552 show homology to TW5d12 bases 1 to 58 (58 in longest run, maxScore = 35). Close enough to tw5d12 to classify as duplicate, 17d8.2 has priority.
PCR Comments :



Length of sequence : 664  Consensus sequence is :

..

CTCGAGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCGTAAT 
CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA 
CACAATATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 
AGTGAGCTAA CTCACATTAA TTGCGTTTCG CTCACTGCCC GCTTTCCAGT 
CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCCGCCA ACGCGCGGGG 
AGAGGCGGTT 
(break)
CAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 
GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC 
GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT 
CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT 
TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG 
TGAATTCCTC GAGGCGCGTG CGACAGAACT GAAGATTGTC AGAGAGAGAG 
AGAGAGAGAC AGAGAGACAG AGAGAGAGAG ACAGAGAGAG AGAGAGAGAG 
AGGGACCAAC AAGGTTTGTG TGTTGGATGT GTGTGACAGA ACTGAAGATT 
GTCAGAGAGA GAGAGAGAGA G

Go back to index

wmc(17e8) Plate reference : 17e8
WMC locus number : WMC Length of sequence : 274
Last updated : Mon, Dec 8, 1997 at 6:09 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 274  Consensus sequence is :

..

AGACAGACAG AGAGGAGCCA TCCACCTCGA TTGCAGCTGT GTTCGCTGTG 
GCTCCGGCCA AGCTGCCCGG TCGATGCAAC AAAGCTGCTC CCACCGTTGT 
GCCTTGTAAT CACGCAGCAG CCATACGTAG CACGGGTCGC CGCCGCCGCC 
GCCGAGGAAG ACGCATAGGC ACCCGTCGGG CTCCGTCGTC CTGAGACTGA 
GAGGATCTTT CCCCAGAGGG CATGGCGGCA GTGGCAAGGA ACCAAAGGTC 
TCGTCCCTGA CGTTGAAAGT GAGG

Go back to index

wmc(17f8) Plate reference : 17f8
WMC locus number : WMC Length of sequence : 281
Last updated : Mon, Dec 8, 1997 at 6:09 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 281  Consensus sequence is :

..

CGGCGGCAAG CGGAAGTGGC GAAATGCGGT GGGCTTTGCC AGCAGCCTGG 
CGGTGGCGGC AGGCGGCGAG CTAAGTGTTT ACCACCATGA TAGGCGGTGC 
CATGGCATAG ACGCACAGGA GCTCACTCTT CGCTTTTTTC ATGTTGAGCC 
TGTCTTCTTT GCTAAGTTTG AAAGCCCTCT TGTGCGGGAC ATTAACACCT 
ATACCCTTTG CACGGCCAGG GGGATCTTTT GTTTCCAGAG CAATGGATAG 
AATGTCACAT GGCCCTGAGT TCCTCTAGAA C

Go back to index

wmc(17g8) Plate reference : 17g8
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Dec 8, 1997 at 6:09 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No sequence 7.11.97
PCR Comments :
No sequence available for this clone
Go back to index

wmc(17h8) Plate reference : 17h8
WMC locus number : WMC Length of sequence : 91
Last updated : Mon, Mar 16, 1998 at 2:04 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB linker corrupted at both ends). Bases 56 to 91 (complement) show homology to 8a4 bases 58 to 93 (36 in longest run, maxScore = 26) - near duplicate. (GA)8 19 to 34, (GA)9 37 to 54.
PCR Comments :



Length of sequence : 91  Consensus sequence is :

..

CAAAACTGAA GATTGTCAGA GAGAGAGAGA GAGACAGAGA GAGAGAGAGA 
GAGATAGAGA TGGAGGGAGA GGGACCAACA AGGTTTGTGT G

Go back to index

wmc(17a9) Plate reference : 17a9
WMC locus number : WMC Length of sequence : 246
Last updated : Wed, Mar 18, 1998 at 10:31 am
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 150 to 169 show homology to 5e12 bases 683 to 702 (20 in longest run, maxScore = 25). No substantial microsatellite, interspersed (GT) repeats, don't bother for now.
PCR Comments :



Length of sequence : 246  Consensus sequence is :

..

TGTTGTGGCG TGTGTGTCTG TGTCTGTTGT GTGGGTGTGT GTTGATGTGC 
AGTTGCGTGT GTGCTATTGT CGCTGTGTGT GTTACTGCCC CGCACTAATG 
TTATGTGTTA TTGTCCCACA CACGCACATA CTACATGATG GGTAAAATGG 
TCTTTTCAGG TGTCATTTAG GCTTAAAATG AACAAGAATG TTATAAAGGG 
CATATTTATA CTCGTTGTTA AATAGAGAAG ATTTTTTTTG TGTGTC

Go back to index

wmc(17b9) Plate reference : 17b9
WMC locus number : WMC Length of sequence : 212
Last updated : Mar 30 mai 2000 at 9:54
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 169 to 212 show homology to 4b7 bases 116 to 159 (44 in longest run, maxScore = 31). No substantial microsatellite, small interspersed sequences, don' bother for now. Bases 86 to 103 show homology to TW3a05 bases 313 to 330 (complement) (18 in longest run, maxScore = 24).
PCR Comments :



Length of sequence : 212  Consensus sequence is :

..

AATATTGATC TTACCTTTAG CTCCTTGTGG GTTCGACACT CCATACTTAT 
CACTTCCACC TTTGGAAATT GCTACGATGA TTCCTTGCAC TTGGGGATTA 
TCACACACAC ACACACTAAG ACTCCATCTA TCTCTCTCTC CCACACACAT 
ACACACACAC ACACACTGAG ACTCCATCTC ACACACACAT GCTCTAGGCG 
GAGCATTTGT TC

Go back to index

wmc(17c9) Plate reference : 17c9
WMC locus number : WMC Length of sequence : 128
Last updated : Mon, Mar 16, 1998 at 2:06 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to insert at 3' end. (CA)14 1 to 28. No room for primers.
PCR Comments :



Length of sequence : 128  Consensus sequence is :

..

CACACACACA CACACACACA CACACACAAG CATGATGAAC ACACACACAC 
ACGTCTGGGT GGGTGGGTGG AATTGAGTCC GAACCCCTAC CCAATCTGAG 
ACCCAGGGGC CGAGGTGTTT CCAATATT

Go back to index

wmc326(17d9) Plate reference : 17d9
WMC locus number : WMC326 Length of sequence : 424
Last updated : Mer 14 juin 2000 at 10:34
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end, and to Ssp1 at 3' end. (CT)16 112 to 143. Bases 60 to 99 show homology to TW4f04 bases 223 to 262 (complement) (40 in longest run, maxScore = 52). Bases 33 to 144 show homology to TW1b07.1 bases 76 to 187 (complement) (112 in longest run, maxScore = 70).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
ggAgcATcgcAggAcAgA at 22 length 18 bases, Tm 63
Reverse primer details :
ggAcgAggAcgccTgAAT at 190 length 18 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 186 bases.



Length of sequence : 424  Consensus sequence is :

..

GTTGGTCTTT GCCGATATGA GGGAGCATCG CAGGACAGAC ACGAGAAGCG 
CGTCCTCCGG GATCCACTAG CGCACGCTTG CCCGACGCGA TGCCAGCGCC 
TCCCCCGCTG ACTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCGCGCGC 
GCGCCCGTTG TTGTTCGCAG CGGGCGGCCC ACGCCTTAGA TTCAGGCGTC 
CTCGTCCGCG GAGGTGTCAA CGAGGGCACG AGTACCCAGC GGTGGCAGGG 
GACGCCCTCT GTCATTGGTA CAGTAGGATT GAAGACAAAT TTATGATTTT 
GAAAGGGCAA ACAATACACA CACCACTAAC AATCCGCGTA ATTGTCCTTT 
TTTAGCTCAT ATGATAACCT TTTTCATCAT GTGACTTTGC ACGCACGCAC 
GTACTCCCTC CGTTCCTAAA TATT

Go back to index

wmc327(17e9) Plate reference : 17e9
WMC locus number : WMC327 Length of sequence : 366
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 15 show homology to 1H3 bases 116 to 130 (15 in longest run, maxScore = 24), zone is 1-40. Primers are outside of this region. (GT)25 150 to 199.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Forward primer details :
TgcggTAcAggcAAggcT at 75 length 18 bases, Tm 65
Reverse primer details :
TAgAAcgcccTcgTcggA at 240 length 18 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 183 bases.



Length of sequence : 366  Consensus sequence is :

..

CGTGAGGCTG GATGTCAACC AACCATGCAA AACCCTAAAC CCGTATTTTA 
ACTTAGGCAA CACGACACTC CCTATGCGGT ACAGGCAAGG CTACTCCTCG 
GAGACGGCAC CCTTCTTGCA GAGCTCCTGG TCGTTGGTGA CTTCCCTCTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
AAATGGGGAG TGAGGGGGTC TGAGGTGGGG GCGGATGGAT CCGACGAGGG 
CGTTCTAGCG GAGCTCCGGC AACGTGTCGC CGGCGTCGCT GGGGAGGAGC 
GGGAGTGGAT GGGGGAGGGC CGGTGCCGGA TGATGCGCAG GGTGCTCCGT 
GGCGTCATCA CCTTCA

Go back to index

wmc328(17f9) Plate reference : 17f9
WMC locus number : WMC328 Length of sequence : 191
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 15 to 83 (complement) show homology to 6d3 bases 243 to 311 (69 in longest run, maxScore = 58), zone is 1-83. Bases 23 to 50 show homology to 19g2 bases 70 to 97 (complement) (28 in longest run, maxScore = 45). Forward primer has mismatch to 6d3 and 19g2. (CT)10 103 to 122. Bases 50 to 101 show homology to 26h4 bases 323 to 374 (complement) (52 in longest run, maxScore = 79).
PCR Comments : 61C anneal, product.
Forward primer details :
ATTgcAgTTcTAcccTcAgT at 4 length 20 bases, Tm 56
Reverse primer details :
gggTcgAcATccATAcATAA at 149 length 20 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 165 bases.



Length of sequence : 191  Consensus sequence is :

..

AATATTGCAG TTCTACCCTC AGTTTGTCTA GTAGGCCTCT CCCACCATCT 
TCGAGAAGCA ACTCCATCAC CCATCCAGCA CTCTACCCCT CTCCCTCCCT 
CCCTCTCTCT CTCTCTCTCT CTGTCCCAAC CTATTTCCCG TCCTTCAGTT 
ATGTATGGAT GTCGACCCAT CTCCCTCAAG ACATAGCTGG G

Go back to index

wmc(17g9) Plate reference : 17g9
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Dec 8, 1997 at 6:10 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No sequence 7.11.97
PCR Comments :
No sequence available for this clone
Go back to index