Wheat microsatellite WMC 25/06/01

wmc(17h9) Plate reference : 17h9
WMC locus number : WMC Length of sequence : 100
Last updated : Mon, Dec 8, 1997 at 6:10 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments : 


Length of sequence : 100  Consensus sequence is :

..

CAGAATAAGC CAAAACCAAC CGACACACAC ACACCCAAGG ATGGATACAA 
GGACGCTGAA AAAGCAACAC CACCCCAACA AACTCGAAGC AAACATCAGA
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wmc(17a10) Plate reference : 17a10
WMC locus number : WMC Length of sequence : 167
Last updated : Wed, Mar 18, 1998 at 11:20 am
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 90 to 126 (complement) show homology to 4f9 bases 264 to 300 (37 in longest run, maxScore = 45), zone is 67-167. (GT)29 35 to 92. Sequence is surrounded by small imperfect repeats. Too difficult to design primers.
PCR Comments : 


Length of sequence : 167  Consensus sequence is :

..

TCGAGGTGCC ACCCTACTCC TTCGGTGTCG GGTAGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTTTGAGAGA 
GAAGCCAGTC GATAGATTAG TATCAAAATC GAGGTGTCAC CCTTCTCCCT 
CGGTGTCGGG GAGTGTG
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wmc(17b10) Plate reference : 17b10
WMC locus number : WMC Length of sequence : 320
Last updated : Wed, Mar 18, 1998 at 11:20 am
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)18 270 to 305. No room for primers.
PCR Comments : 


Length of sequence : 320  Consensus sequence is :

..

AATATTTAGG AATGGAGGGA GTACATCTTA GGCAGCATGT CATCCACAAA 
AGGCATAGTC TGGGCCAAAC CAACAGCTCA CTTAGGAGCC CGTCTTCTTC 
ACAGGTAACA ACAAAAAAGA TGAGATAGTT TGCAGAATTA TGCTCACGTT 
ACACATAGAA GCTGCTTTAT TTGGCACTCC AGTGATCCAG TTCACACACA 
TGAAAAACCA CAGTACAAAT AAATGCTCGG TCGAGTCTGA ACTCTGAACA 
TAATTTAGCT AGTGACACTC ACACACACAC ACACACACAC ACACACACAC 
ACACAACACA CACACACACA
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wmc(17c10) Plate reference : 17c10
WMC locus number : WMC Length of sequence : 401
Last updated : Mar 10 Nov 1998 at 14:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 214 to 259 show homology to 11h3 bases 353 to 398 (46 in longest run, maxScore = 41), zone is 189-287. (GT)12 32 to 55, (GT)8 189 to 204, (GT)8 272 to 287. Sequence is surrounded by small minisatellite like repeats. Too difficult to make primers. Bases 45 to 109 show homology to 21c3 bases 80 to 144 (complement) (65 in longest run, maxScore = 46), duplicate with 21c3, 17c10 has priority.
PCR Comments : 


Length of sequence : 401  Consensus sequence is :

..

GGGGGTGCTC TCTCTCTCTC TGTGTGTGTA TGTGTGTGTG TGTGTGTGTG 
TGTGTTTTAT TCGTGTCCCC TAGTTGCAAG TTGACCATCA ACTCACAACT 
AGGGGTCTTT GTGTGTGTGT GTGTATCTTT TGTTGTGGCC CCCGGTTGCA 
ACCCGCTCAT CAAGTCGCAA CTAAGGATGC GCGCGCGCGT GTGTGTGTGT 
GTGTTTGTAG AGGGTGTGTG TTTTGTTGTG GCCCCCAGTT GCACCCCGCT 
CATCGACTCG CAACTAAGGA TGTGTGTGTG TGTGTGTCAC TAGTTGCATG 
TTGATAACCG ACTCACAACT AGAGGTGTGT GTGTGTGTGC GTCTTTTTGT 
CAAGGGTCCT TAGTTGCATG TTGACCATCA AACTCGCAAC TAGCAGGTGT 
G
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wmc(17d10) Plate reference : 17d10
WMC locus number : WMC Length of sequence : 287
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 95 to 112 (complement) show homology to 3f3 bases 425 to 442 (18 in longest run, maxScore = 57). No substantial microsatellite. Bases 235 to 267 show homology to 25g1 bases 146 to 178 (33 in longest run, maxScore = 58).
PCR Comments : 


Length of sequence : 287  Consensus sequence is :

..

CATGCATCAA CACACACATT TTGACACCCT CACCCGGCCC CGGCCCACCT 
CAAGGCCCGC ACATCGTTCT CTATCTTGCA CGCACAATGT CTTCGTGAAA 
ACCCTATTTA GCAGGTAGCT TTCCATCACA TGCGCACACA TTCTCTCCGG 
TTTCATTTCT CTCAATATCT GACACACACA CACACTCCCC CTCCCTCGAG 
CACACAATTC ATCCCCACCC AAGGTTATAT GGCAACCACT CTCGCTTGTG 
CTTCTCTGCA CCCCAGCATG CACACCACCT CAATATT
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wmc(17e10) Plate reference : 17e10
WMC locus number : WMC Length of sequence : 159
Last updated : Wed, Mar 18, 1998 at 11:37 am
Owner : Khairallah, Mexico Date at Agrogene : 19.12.97.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 97 to 136 (complement) show homology to 13b6 bases 87 to 126 (40 in longest run, maxScore = 76). Duplicate of 17f10. (CA)8 37 to 52, (CT)8 21 to 36. Member of 1g1 family.
PCR Comments : 


Length of sequence : 159  Consensus sequence is :

..

CACACAAACT AAGACTCCAT CTCTCTCTCT CTCTCTCACA CACACACACA 
CACCCCACAC ACACTAAGAC TCCATCTCAC ACGCACATGC TCTAGACGGA 
GCATTTGTTC CTACCACCCT TCATCCAACC TTACCCATAT GATAAACAAT 
CACCTTTAT
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wmc(17f10) Plate reference : 17f10
WMC locus number : WMC Length of sequence : 159
Last updated : Wed, Mar 18, 1998 at 11:37 am
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Duplicate of 17e10, member of 1g1 family. (CA)8 37 to 52, (CT)8 21 to 36.
PCR Comments : 


Length of sequence : 159  Consensus sequence is :

..

CACACAAACT AAGACTCCAT CTCTCTCTCT CTCTCTCACA CACACACACA 
CACCCCACAC ACACTAAGAC TCCATCTCAC ACGCACATGC TCTAGACGGA 
GCATTTGTTC CTACCACCCT TCATCCAACC TTACCCATAT GATAAACAAT 
CACCTTTAT
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wmc329(17g10) Plate reference : 17g10
WMC locus number : WMC329 Length of sequence : 255
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 78 to 107 show homology to 11b10 bases 167 to 196 (30 in longest run, maxScore = 55), zone is 78-255. Reverse primer has two mismatches to 11b10, forward primer is not within the zone of homology. (GT)21 68 to 109.
PCR Comments : 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide (beware of primer dimer).
Forward primer details :
AcAAAggTgcATTcgTAgA at 16 length 19 bases, Tm 56
Reverse primer details :
AAcAcgcATcAgTTTcAgT at 115 length 19 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 118 bases.


Length of sequence : 255  Consensus sequence is :

..

CTCCTTTCGT AACTTACAAA GGTGCATTCG TAGAAAATTA GAAACAAATA 
AATCATGCAT TGAATTGGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTT GAAAACTGAA ACTGATGCGT GTTTAATCGC ATATAGAACG 
CTATAATAAA TCTATTTTGA AATTAGACTC AAACACCTCT CAAGTTGGTT 
TTTAAAGTAG AACAGTGCAT TTGAAGACGA AACAAACATA GAGGGAGAAA 
TCTAT
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wmc(17h10) Plate reference : 17h10
WMC locus number : WMC Length of sequence : 372
Last updated : Mon, Mar 16, 1998 at 2:17 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 298 to 372 (complement) show homology to 6a11 bases 4 to 78 (75 in longest run, maxScore = 104), zone is 207-372. (CA)8 149 to 164, (CA)8 202 to 217, (CT)11 54 to 75. Many small microsatellites. Too difficult to design primers.
PCR Comments : 


Length of sequence : 372  Consensus sequence is :

..

CACGCACACA CACATGCTCG CACCCCTCCC ACACACACAC ACTAAGACTT 
CATCTCTCTC TCTCTCTCTC TCTCTCACAC GCACCCACGC ATGAACGCAC 
CCCCCCCCCA CACACACACA CACTAAGACT CCATCTCTCT CTCTCTCGCA 
CACACACACA CACAAACTAA GACTCCATCT CTCTCCCCTC TCTCTCTCTC 
TCACACACAC ACACACACTA AGAATTTATA TCACACACAC ATGCTCTAGT 
CGGAGCATTT GTTCCTACCA CCATTCATCC AACCTTACCG TATGATAAAC 
AATCACCTTT ATTTACTTGC ATAACATGGA CAAATTCCAC TTTACTTTAG 
TAGTCAGCAA ACTTATCATC TG
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wmc330(17a11) Plate reference : 17a11
WMC locus number : WMC330 Length of sequence : 156
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). Bases 1 to 34 show homology to 4a10 bases 98 to 131 (34 in longest run, maxScore = 32), zone is 1-72. Forward primer has 1 mismatch to 4a10, reverse primer is outside this region. (GA)15 46 to 75, (GA)13 88 to 113.
PCR Comments : 61C anneal, smear; 51C anneal, smear.
Forward primer details :
gcgTgcgAcAgAAcTAAAgA at 20 length 20 bases, Tm 61
Reverse primer details :
cAcAAAccTTgTTggTcccT at 135 length 20 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 135 bases.


Length of sequence : 156  Consensus sequence is :

..

CAAGGTTTGT GTGTTGGATG CGTGCGACAG AACTAAAGAT TGTCAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAGACAGAG AGAGACAGAG AGAGAGAGAG 
AGAGAGAGAG AGATAGAGAT AGAGATGGAG GGAGAGGGAC CAACAAGGTT 
TGTGTG
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wmc(17b11) Plate reference : 17b11
WMC locus number : WMC Length of sequence : 175
Last updated : Lun 2 Nov 1998 at 10:04
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). Bases 54 to 74 show homology to 3e12 bases 225 to 245 (21 in longest run, maxScore = 34), zone is 45-110. (CA)12 97 to 120. Sequence is full of small repeats (minisatellite like). Too difficult to design primers. Bases 59 to 93 show homology to 24d3 bases 161 to 195 (complement) (35 in longest run, maxScore = 54).
PCR Comments : 


Length of sequence : 175  Consensus sequence is :

..

CACACACCTA TTGCGAGTCG ATTATCGACA TGCAACTAGG GACACTAGTT 
GCAAGTCGAT GCTTGACATG CAACTAGGGA CCCCTTGACA AAATGACACA 
CACACACACA CACACACACA TCTCTAGTTG CAAGTTGGTT ATCGACATGT 
AATTGGGGAC CCTCGACACA CACAC
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wmc(17c11) Plate reference : 17c11
WMC locus number : WMC Length of sequence : 6
Last updated : Mon, Dec 8, 1997 at 6:11 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No sequence 7.11.97.
PCR Comments :
No sequence available for this clone
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wmc(17d11) Plate reference : 17d11
WMC locus number : WMC Length of sequence : 183
Last updated : Wed, Mar 18, 1998 at 12:09 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' and to insert at 3' end. Bases 14 to 51 (complement) show homology to 5a4 bases 189 to 226 (38 in longest run, maxScore = 44), zone is 87-183. (GT)10 147 to 166, (CT)21 103 to 144. No room for primers.
PCR Comments : 


Length of sequence : 183  Consensus sequence is :

..

AATATTTAGG AATAGAAGGA GTATTTTTGA ATTCGTGTCA TACATGCATG 
GGTTGGAAAA TAGATGCACT ATACTTATTG GATTGTTGTT GTGTTCGTGC 
GTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTATGTGT 
GTGTGTGTGT GTGTGTGGTC ATATGCTTGA TAC
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wmc(17e11) Plate reference : 17e11
WMC locus number : WMC Length of sequence : 302
Last updated : Mon, Mar 16, 1998 at 2:22 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to Ssp1 at both ends. Bases 176 to 193 show homology to 5A1 bases 319 to 336 (18 in longest run, maxScore = 62). Bases 249 to 265 show homology to 12f6.2 bases 341 to 357 (17 in longest run, maxScore = 79). (GT)14 250 to 277. No room for primers.
PCR Comments : 


Length of sequence : 302  Consensus sequence is :

..

AATATTTTGA ATAAGAGATC CTGATGTTTG AAACCCATGC ATGCATGAAT 
ATAACGGAGA TATGCGCGGT GTGGTTTGGA AACGAACATG TTGTAATGTA 
TACATATTGA ACAAGGTCAG ACTCATTTGA ATTTGAGATA CCGATGGCAG 
ACATCATTTG GCCACGTCGC ATCCGTGCAT GGACACACTA TTCTAATTGG 
AGGTGATGGG CGGCTTTCGC ATCACATATC TATATCTATA CCCCTATATG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGCT GTGTGTGTGT GTGTATAATA 
TT
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wmc331(17f11) Plate reference : 17f11
WMC locus number : WMC331 Length of sequence : 363
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to insert at 3' end. (CA)13 56 to 81, (CT)10 36 to 55. Software struggled to get these primers.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
ccTgTTgcATAcTTgAccTTTTT at 2 length 23 bases, Tm 61
Reverse primer details :
ggAgTTcAATcTTTcATcAccAT at 107 length 23 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 128 bases.


Length of sequence : 363  Consensus sequence is :

..

CCCTGTTGCA TACTTGACCT TTTTTGTACG CTAAACTCTC TCTCTCTCTC 
TCTCTCACAC ACACACACAC ACACACACAC AATAGTTTCA TTTCACACAT 
TCATCTATGG TGATGAAAGA TTGAACTCCA TATGATCTCT TTAGGTTCAT 
ATCTATTATA TTCTAGGCTT TTTCTAGGGG TTTCCCAAAA AGGAGTTATG 
AAGGTATAGT AAAAAAATCC AAAACATGCT AAAAGCATCC TTGTTGCTTG 
CTCAAGCATA ATCAACTTGG TTTGATCATG CAATTGTTAT TTGCTAGGGC 
TTACACTTTG TAGGACCGAA GCAACTTATT ACACACTTGA CATTATTTAT 
CGGCTCTAAT ATT
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wmc(17g11.1) Plate reference : 17g11.1
WMC locus number : WMC Length of sequence : 334
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Multiple inserts in this clone, this is first insert. Trimmed to insert at both ends. Bases 62 to 143 (complement) show homology to 13c5 bases 229 to 310 (82 in longest run, maxScore = 125). No substantial microsatellite (several small CA repeats).
PCR Comments : 


Length of sequence : 334  Consensus sequence is :

..

GCGGAGAGGG GTGTGGGTAT CTTTTTGCAA AATTGCCACA GTTTCCTTCC 
TATCCGTCGG ATATAAATCG GATGGCCTAT ATTGCAGGAT GGCAGGAACA 
CCATCATCAC CAACTCTGTT TTTTATAAGA GTAGGGATAT GTATGTGTCA 
CTGCCCCCCC GACACACACA CCCACACCCA CACAACCACA CACACACACA 
CTTCCCAACA CCGAAGGAGT AGGGTGACAC TTCGATCTTG ATACAAATCT 
ATCGACTGGC TTCTCTCTCA AACACACACA CACACGCACA CACACTACCC 
GACACCGAAG GAGTAGGGCG GCACCTCGAT CTTG
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wmc(17g11.2) Plate reference : 17g11.2
WMC locus number : WMC Length of sequence : 188
Last updated : Mon, Dec 8, 1997 at 6:11 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Multiple inserts in this clone - this is second insert. Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments : 


Length of sequence : 188  Consensus sequence is :

..

CCATGCCGCA CCGCTCGCGC ACCCGAAGCA GAGCCCGAGT TGAGCTCGCG 
CCCTAGATCA CCCCTTGCGC GCCCGACCCG CCGCACGCGA GGAAAACCCA 
TAGGGGAACC CTACCGCAGC CTCTCCCCAC TTTTCGTCGT TCGCACGCAG 
CCGACGGGCG AAACAGATCT CGCCGAAGGA GCTCGAGA
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wmc(17h11) Plate reference : 17h11
WMC locus number : WMC Length of sequence : 60
Last updated : Mon, Dec 8, 1997 at 6:11 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No insert - only vector sequence.
PCR Comments : 


Length of sequence : 60  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC TCTAGAGTCG ACCTGCAGGC 
ATGCAAGCTT
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wmc(17a12) Plate reference : 17a12
WMC locus number : WMC Length of sequence : 428
Last updated : Mon, Dec 8, 1997 at 6:12 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimed to Ssp1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments : 


Length of sequence : 428  Consensus sequence is :

..

AATATTTCGT GGCGCTGCCA TTACAACGGC GGGTTCAAGG AGTTCCCTGA 
CCGGACACTC GAGACAGTGA GCTTGCTGTC AACAACCACA AGGGCGGCGG 
CGGCGGCAGA CGGACAACAA CATCCGGTGC TGCGCATTGA CGCAATCAAG 
GTCAGTTCAT CACTGGCAGG AGTACACTCT ACTTCTCAAG AATCCCAAGT 
TCCCAACCCA AAGTTTTCAG GCTAATCTTA TTTATTACTC TCGCTGACAG 
GTGGAACGTG TGCAATGAAG CATACATATT GTTTCTGCAA ATCTGCCTAA 
GGACCAAGAA AAGTAAAAAG TGCACCTTTG GTTTATTAAG TGTCTATTCA 
TCCTGCCGCC CCCTCCTCTA GACCTATCTT CTCGATCCTA CAAACTATCT 
TTAGGGCCTT TTTGGACTAA TATGTGAC
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wmc332(17b12) Plate reference : 17b12
WMC locus number : WMC332 Length of sequence : 301
Last updated : Mar 17 Nov 1998 at 11:00
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimed to insert at both ends. (CT)12 136 to 159.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
cATTTAcAAAgcgcATgAAgcc at 51 length 22 bases, Tm 65
Reverse primer details :
gAAAAcTTTgggAAcAAgAgcA at 198 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 169 bases.


Length of sequence : 301  Consensus sequence is :

..

GCGACGAATA GAATGGATCT AACATTGTAG GATGTCTTGT GGTATAGTTC 
CATTTACAAA GCGCATGAAG CCCATTTAAC GAGCTAGGCA GTATTATCTC 
TTATGATATT TTCTCTAAAA GCTGGCATCT TATGACTCTC TCTCTCTCTC 
TCTCTCTCTG TGTGTGTGTG TGTGAGTGTG TGTGTGTGGA AATCTTATGC 
TCTTGTTCCC AAAGTTTTCT GTTGTGTTTT GTTCGCTGCG CTGCTGCATC 
GTGATTCCCT GGTTTTCTAT TTCTCGTTTT TTGTTTAGGG TTTTTTTGTT 
G
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wmc(17c12) Plate reference : 17c12
WMC locus number : WMC Length of sequence : 181
Last updated : Mon, Mar 16, 1998 at 2:35 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 115 to 181 show homology to 6d10 bases 109 to 175 (67 in longest run, maxScore = 57). (CT)18 66 to 101. Too difficult to design primers.
PCR Comments : 


Length of sequence : 181  Consensus sequence is :

..

CACGCATCTC ATGTATATTA CATGTATCCA TCTTTCTCAC AGACCTAACT 
TCTTCCTCTC TCTTTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC 
TATCTCTCTC TGTCTCATTA GGTTTGTCTC CTACTCTCTC GTCCACATAC 
ATACAATCTT TCCCGCCCTA TCTGCTCCAT C
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wmc(17d12) Plate reference : 17d12
WMC locus number : WMC Length of sequence : 268
Last updated : Mon, Dec 8, 1997 at 6:12 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments : 


Length of sequence : 268  Consensus sequence is :

..

GCACTTGCCT CCCATCTGAG CTTGCTCGCG GTGGTCGGCG GCGGCGGCTA 
GGGTTTCGGC TCCGAGGGGA AACGAACCCT GGAGAGGGGA AGTATGGAGA 
GAGGAGGAGG ACGGGAAGGG ACAGAGGGTT TGGTATAGGC TTGACTGGGC 
CGAGCGTGAA TCCCGGTTAT CGTCATTGTC TGCACAAATG AGAAGCCCAA 
TTCCCCAAAG GGCGCAGTTG CTTTTTATGA AAGCCCAATT CTTTCGGCCG 
ATTCTCCCGG AATCTTCG
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wmc(17e12) Plate reference : 17e12
WMC locus number : WMC Length of sequence : 502
Last updated : Lun 5 juil 1999 at 17:22
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Forward and reverse sequence join is approximate (hence estimates of fragment sizes). Trimmed to insert at both ends. Rsa1 site at 7. Bases 95 to 203 (complement) show homology to 2d9 bases 16 to 124 (109 in longest run, maxScore = 83), zone is 94- 217. (GT)9 70 to 87, (GT)11 205 to 226. Too difficult to design primers. Bases 172 to 187 show homology to 10f2 bases 198 to 213 (complement) (16 in longest run, maxScore = 31). Bases 205 to 230 show homology to 24b2 bases 26 to 51 (26 in longest run, maxScore = 49). Bases 296 to 314 show homology to 20g9 bases 240 to 258 (complement) (19 in longest run, maxScore = 38). Bases 355 to 375 show homology to 10d5 bases 143 to 163 (complement) (21 in longest run, maxScore = 55).
PCR Comments : 


Length of sequence : 502  Consensus sequence is :

..

TCGAGGGTAC CCAGTTGCAT GTCAACCATC GACTCGCAAC TAGGTGTGTA 
TATGTGTGTG TGTGTCTCTG TGTGTGTGTG TGTGTGTGGA GGGTCCCCAG 
TTGCATGTCG ATCATCGACT CACAACTAGG GAAGTGTGTA TTTTGTCAAG 
GGTCCCCAAT TGCAACCTGA CCATCGACTC GCAACTAGGT GTGTGTGTGT 
GTGCGTGTGT GTGTGTGTGT GTGTGTTTGT CGTGGGTCCT CAGTTGCATG 
TCAACCATCG ACTCGCAACT GTGTGTGTGT TTCGAGGGTC CCCCAGTTGC 
ATGTCAACCA TCGACTCGCA ACTAGGGGTG TGTGTGTTTG TCGAGGGTCC 
CCAATTGCAT GTCAACCATC AACTCGCAAC TACGGGTGTG TGTGTGTTTG 
TCAAGGGTCC CCCATTTGCA TGTCAACCAT CGACTCGCAA CTAGGGGGTG 
TATGTGCGTT TGTCGAGAGT CCCCAGTTGC ATCTCAACCA TCGACTCGCA 
AC
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wmc(17f12) Plate reference : 17f12
WMC locus number : WMC Length of sequence : 177
Last updated : Mon, Mar 16, 1998 at 2:39 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 12 to 43 (complement) show homology to 5c12 bases 146 to 177 (32 in longest run, maxScore = 40). (TTG)14 65 to 106, (TTG)10 148 to 177. No room for primers.
PCR Comments : 


Length of sequence : 177  Consensus sequence is :

..

GTTGGTGTTG CTGTTGCTGT TGCTGTTGCT GTTGCTGTTG CTGTTGCTGC 
TGTTGCTGTT GCTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG 
TTGTTGTAGT TGTTGCTGTT GCTGTTGTTG TTGTTTTGTT GTTGTTATTG 
TTGTTGTTGT TGTTGTTGTT GTTGTTG
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wmc(17g12) Plate reference : 17g12
WMC locus number : WMC Length of sequence : 60
Last updated : Mon, Dec 8, 1997 at 6:12 pm
Owner : Khairallah, Mexico Date at Agrogene : 7.11.97 Public Domain : Yes
Comments : No insert - vector sequence only.
PCR Comments : 


Length of sequence : 60  Consensus sequence is :

..

GAATTCCTCG AGGCGCGCCT CGAGGGATCC TCTAGAGTCG ACCTGCAGGC 
ATGCAAGCTT
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wmc(17h12) Plate reference : 17h12
WMC locus number : WMC Length of sequence : 186
Last updated : Fri, Dec 19, 1997 at 9:38 am
Owner : Khairallah, Mexico Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Forward sequence only. Trimmed to insert at both ends. Bases 110 to 186 show homology to 4b7 bases 178 to 254 (77 in longest run, maxScore = 102). No substantial microsatellite.
PCR Comments : 


Length of sequence : 186  Consensus sequence is :

..

CACTAAGCAG ACTTCATCTC TCTCCCCCCC CACACACACA CACACTGAGA 
CTCCATCTCA CACACACATG CTCTAGGCGT AGCATTTATT CTTACCACCC 
TTCATCCACC CTTACCCGTA TGATAAACAA TCACCTTAAT TTACTTGTAT 
AACATGGACA AATTCCACTT TACTTTAGTA GTTAGC
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wmc(12A1) Plate reference : 12A1
WMC locus number : WMC Length of sequence : 545
Last updated : Tue, Dec 30, 1997 at 10:09 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. No substantial microsatellite.
PCR Comments : 


Length of sequence : 545  Consensus sequence is :

..

GTACTAACGT TGCCAAGATT TTGCGCTAGC CCTAACCTTT TGCACGTGGC 
TTTGTTTGCT TGCGCCAGGA GACTCTCGTC TCATCAGTAG TAATATCAGT 
GTGGGATTTG TCAGAGGAAA AACAGGGTGA AAGTTGCTCC AGCCCATGCC 
ACTAGCATGC ATGCCTTGGA TGCTTGCACC ATGGACGATC ACCGTATTGA 
TTACCGGCCA AGAACCACTA GGCTGGAGAT TGAGCGACCC GTGCTGCTAG 
CATTGGGCTC ACCCTGATAG ATCAATCCCA CCCAATTCTC ACCCTGTGTG 
TGTAGCTAGG TGATCGGAAG CGCCGGCGGC GGCGATCTGC TTTGGGGCGG 
CGTTGGTGGC GGCGTTCGAG TCCGTGGTGG CGCAGGCGGT GGTGATTCAT 
TCGGCTGTAG ACCAGTGGGG CGAAGTGGTT ACCGGAGAGC ATTTCTTTGG 
TGGTAAAATT CCACGTTCAT CGCTGGTTTG TGCAGCTTAG GCGGCGTGGA 
TCGTGGCGGC GGCTGGATCT GAGAGGCAGG AGTGGTTGCT GGTAC
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wmc(12b1) Plate reference : 12b1
WMC locus number : WMC Length of sequence : 520
Last updated : Tue, Dec 30, 1997 at 10:09 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments : 


Length of sequence : 520  Consensus sequence is :

..

GTACAAAAAA GATATTAACT ATATCAGCTA AAACACTTGA GTAAACAAAC 
AATAATCTTC AGTAAACAAA GCACTAAAAT CAAACTGCCC TAAAATCTCC 
TAAAATCTAA CAACCCTAAA ATTTCCTAAA ATCTAACAGC CCATTAACAA 
CCCTACTGTA ACATGATAGG TTTATATGCA ATCTAACAAC CCTAAAATTT 
CCAGTGAATC CAATGAAGAG AGGGGTGGAG GGGGAAGAAA TCTTACCGCA 
CGCCGCCGAA CCCTGAAGCC CACTGATGGC CTTCTCCCAC GCCTGAAGCC 
CTGATCTTGC GTGCTAGGCC CGCCGCCGCA CGGCGCTCTA TGTCCCACTC 
TGGCAGCGAG GGGGGCGAAG GGGATCGCTC CGGCGAAGGA GCCCGCTGCG 
TCGGCAAGCT ACTACTGCCG AACCAGTTGT CCACCTCCGA GAACGCATCA 
CTATCGCCTC CAGTCCCTCC GCCGCCTGCT GACTCGCCGC CGCTGCCGCT 
AGGTTTAGCC GCCGCCGCCA
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wmc(12c1.1) Plate reference : 12c1.1
WMC locus number : WMC Length of sequence : 312
Last updated : Tue, Dec 30, 1997 at 10:09 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts in this clone. This is the first insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments : 


Length of sequence : 312  Consensus sequence is :

..

GTACACCACC GAGAGATCTG TCTCCCTCGC GTGGGTCTCC ATTCTATGCT 
CGCAGAACTC CATTGGAGCG CCACTGGACA CCCCTCTCTA TGTGTCGTCG 
TGGGTGTGCT TGTTGTGTTG TGGGGCGTGA TCGAAAGGTT GAAGAGACTA 
AGGTTATAAT GGCCACGGGA ATTAAAGGGG AAGCCAGACG GCCAGGAATA 
TCGGCAGGCC CTGATGGCAG TTCTAATTTG CAGCGCATAA CTCCATCGCG 
TTGCACGTAA CTGCATCGCG TGGCAACGCA TGGGGCGCAA CCGCATGCAT 
ACCGGGCCCC CG
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wmc(12c1.2) Plate reference : 12c1.2
WMC locus number : WMC Length of sequence : 278
Last updated : Tue, Dec 30, 1997 at 10:10 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts in this clone. This is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 200 to 235 (complement) show homology to 7a2 bases 171 to 206 (36 in longest run, maxScore = 152). No substantial microsatellite.
PCR Comments : 


Length of sequence : 278  Consensus sequence is :

..

GTACACGTGG AAGGAGAAGA GACAACAAGT TTGATGAGAG AGCTGGAAAA 
ACATGGACGA GAGGGGAAGG ATCTCGTGGG TTGAGGGATT GATAAATTTG 
TGCGGGGGAG AGAGGGATTG GTAATTTTGT GCGAGAGAGA GAGGGTGGGG 
GAGGAGTCAG TCAGCCGTCG TCACATCATC CTGCTTCCAA ATGGCCCCAC 
ATTCTCTAGC GCAGTGTGGT CGCCGTCTTC GATCTCCTCG ATGCCCTCTT 
TGAAAATTGA GGTGTTGTGC ATGTTGGG
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wmc(12d1) Plate reference : 12d1
WMC locus number : WMC Length of sequence : 461
Last updated : Tue, Dec 30, 1997 at 10:10 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 17 to 29 show homology to 5b11 bases 72 to 84 (13 in longest run, maxScore = 48). No substantial microsatellite.
PCR Comments : 


Length of sequence : 461  Consensus sequence is :

..

GTACCAATTT GTGTCCACTC CGTATGAGGT GTCTGCAGCT GCTGCAACTT 
TCTTGCGTTG GGAATTGTTC TCGGCATAGC GATAGTCACA ATCCTTGGCA 
ATATGATTAT TCCTTTTACA AATTTGACAT TTATTTTCAT ACTCATCGTA 
ACCTTGGAAC GGGCGTCCCC TGTTGTTGTT GTAGAAGGGG CGCCGGTTGG 
TGTTGTTGGA GTTGTAGTTG TTGCTGTTGC GTTGGTGGTG GCCGCCGCCA 
CCACCACTGC CGCCGCCGGT GTTGCCACTG TTGTTGTAGT GGTTGTTGCC 
GGTGTTGTAG TTGTTCCCGC CGCCGCTGCT ACCGCCACCA CCATAGCTGC 
CGCCGCCGCG AGAGCCCCCG CCACCACCAC CCTTGCGGTA GCCTTGGGGA 
GACCCGCTGC GGCCTCGAGC AGCAAGATTT GCAGATGACT TGAAGGCTCC 
TGCCCCCGTT C
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wmc333(12e1) Plate reference : 12e1
WMC locus number : WMC333 Length of sequence : 323
Last updated : Mar 17 Nov 1998 at 10:54
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (GT)15 187 to 216.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
TcAAgcATAggTggcTTcgg at 73 length 20 bases, Tm 65
Reverse primer details :
AcAgcAgccTTcAAgcgTTc at 227 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 174 bases.


Length of sequence : 323  Consensus sequence is :

..

GTACTTGTGC TTCAAAGACG AGTTTTGGTC ATGGGCCCAA GGATAATTCT 
TCGTGGTGCC ATACCAATTG ACTCAAGCAT AGGTGGCTTC GGCCACCGCC 
CTCTCCTCTA GAGAGGTTTT GGTTAGTGTT TGTTCCTTGT TGCCTTTTAC 
CTTCTTGTTG AGCTGTGTTT GTGTTTCAGT TGCCTCGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTTTGC GTGTCCGAAC GCTTGAAGGC TGCTGTGCAA 
AGGCCGTGTT TCTCTTTTTT TTGAAACATG CATTTTCTTG GGTTTACGGC 
ACTACCGTGG CGAGCCACGG TAC
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wmc(12f1) Plate reference : 12f1
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Dec 30, 1997 at 10:10 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : No sequence 19.12.97
PCR Comments :
No sequence available for this clone
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wmc(12g1) Plate reference : 12g1
WMC locus number : WMC Length of sequence : 337
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite. Bases 159 to 168 show homology to 20h7 bases 129 to 138 (10 in longest run, maxScore = 19).
PCR Comments : 


Length of sequence : 337  Consensus sequence is :

..

GTACAACTGC TGGCCCCTTC GACGTCCATC TCCAGAAGCC AGCGCCTGAC 
CTCCCAGCGG TAGTGACGAA GATCTCCCTG GGGTCATGCC CCTCTCGGAA 
CTCGACGGCG GCCGTGGCGA ACCTCTGCCG CGGTGTCCCG ACGGCGACCT 
GGAGGTCCGC AATGAAGGTG GCGGCGGCTC CTCCAGGACC TCGGCGGCAG 
CAAAGGGATC TGCGATGGCG GCTAGGGCGT GGTTTCCGTC GCCGTAGCGA 
ATAGATCGGC GACGGCGAAG GGATCGGCGG CGGCGATGGC GGCTGGGGCG 
TGGGTTCTCC TAATCTCGTC CTCGTAGCGA GACTGCC
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wmc(12h1) Plate reference : 12h1
WMC locus number : WMC Length of sequence : 322
Last updated : Tue, Dec 30, 1997 at 10:10 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 322 show homology to 5a6 bases 2 to 323 (322 in longest run, maxScore = 263). No substantial microsatellite.
PCR Comments : 


Length of sequence : 322  Consensus sequence is :

..

AGGGTCGAGG AGCAAAGCTT AGTGCGGCTG CCGCCTGAAG TTAGACCTCC 
ACTATCCCGC CATCGACCGC GATGGATGTG CTCGAATCAC GCCGCCGCCG 
ACCTCGATGG TTGAGCTCCT CCTATCTCCC GCCGCAGACC GAGGACTGAC 
CTCCTATGAC GCCGCCACCG CCCACCGACC GAGGATTGAC CGCTTCTCCG 
GTAGCCGCTG CCTAGGCCGC CGCCATCTCT ATATGCAGAG CGGCTGTTTT 
TTTACGGATA TCCGTAGAGC AGCTGTTTAC ACGGTATCTC ACCGTAACTC 
GGTTATATGG ACAGGGGAGT AC
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wmc(12a2) Plate reference : 12a2
WMC locus number : WMC Length of sequence : 355
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 207 to 239 show homology to 7h11 bases 285 to 317 (33 in longest run, maxScore = 28). Bases 206 to 247 show homology to 12b6 bases 120 to 161 (complement) (42 in longest run, maxScore = 145). No substantial microsatellite. Bases 1 to 41 show homology to 26e1 bases 34 to 74 (41 in longest run, maxScore = 74).
PCR Comments : 


Length of sequence : 355  Consensus sequence is :

..

GTACCGCCTC TGGTGCGGCG CCGTGTATGC ATCTGCTGAG AGTTTGACGG 
TGCTGTGGTA GTGATGGTTG TCGGCGGCGT GGAAGCAGAT CTAGGAGGGT 
AGACGGTGGC GGCGGGGCGC GGTGGACAAG ATGGTCGCAG GAGCGGCGCC 
GGCAAGGTGG ACGACGGCGG GGATGAAGTG AGAGGACGGG ATGCAAGGAT 
CCCGTTCCGA AGCCGTGGAT GAGAGAGGTC GATCTCTTGT AATGGACGGC 
GGCGTCGGGG TATGAGCTCC GGCATGGTGG AGGAGGACGG CGGTGGATGG 
GGTGGCGGAC GAAGGAGGCT AGGGTGGAGA GGGTGTTTTG GTGCCCACGG 
AGTAC
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wmc(12b2) Plate reference : 12b2
WMC locus number : WMC Length of sequence : 544
Last updated : Tue, Dec 30, 1997 at 10:10 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 409 to 427 show homology to 4d3 bases 123 to 141 (19 in longest run, maxScore = 43). No substantial microsatellite.
PCR Comments : 


Length of sequence : 544  Consensus sequence is :

..

CACAGTAGCA CAACCTTTCG AGGAATCATC CCCTGTCGGG AAGCATGATG 
TGCCGGGAAT ATCACATTTG AAAGATCGTG GATGTCGCCT AGAGGAGGGG 
GGGTGAATAG GCGTTTTAAA ACAATTACGG TAGAGGCTCG AACAAATGCG 
GAATAAACCT AGCGGTTAAT TTGTCAAGCA CAAAACCTAC AACAACTAGG 
CTCACCTATG TGCACCAACA ACTTATGCTA AGCAAGGTAA GCAACAAAGT 
GATAGCAAGA TATATGACAA ATAACAATAT GGCTATCACA AAGTAAAGTG 
CATAAGTAAA GGGCTCGGGT AAGAGATAAC CGAGGCACGA GGAGACGATG 
ATGTATCCCG AAGTTCACAC CCTTGCGGAT GCTAATCTCC GTTTGGAGCG 
GTGTGGAGGC ACAATGCTCC CCAAGAAGCC ACTAGGGCCA CCGTAATCTC 
CTCACGCCCT CGCACAATGC AAGATGCCGT GATTCCACTA AGGGACCCTT 
GAGGGCGGTC ACCGAACCCG TACTACCGCT CCTGTCCAGG GTAC
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wmc(12c2) Plate reference : 12c2
WMC locus number : WMC Length of sequence : 342
Last updated : Mar 30 mai 2000 at 9:54
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 5' end. (GA)37 114 to 187. Sequence contains numerous small internal repeats (minisatellite like). Too difficult to deign primers. Bases 194 to 278 show homology to TW4c01 bases 148 to 232 (85 in longest run, maxScore = 116). Minor sequence differences to TW4c01.
PCR Comments : 


Length of sequence : 342  Consensus sequence is :

..

GGCTCCCTTG CAACTTGCAA GGTCGTGCGT GCATGGGGGG ATGCATGCAT 
GGATTGTCAG ACGAGCTTGA CTTTGACAAT TCCTCGGGTC GTCGCCAACA 
CCAACACGGT GCAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGGG AGGGAGCGAC 
GGACCAAGAC AATGGCGCGG AGCCAGTGCC CACTGCCCAC TGGCCACGGC 
CTTCGGCGTC GCTTCGCGCT ACAATTTCCA CAAACACCTC GAGCTCGTGC 
GCACGCTGGT AAAAAGGCTA CCTCCAATCC TCTTCCTTGT AC
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wmc(12d2) Plate reference : 12d2
WMC locus number : WMC Length of sequence : 427
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 69 to 113 show homology to 13e4 bases 172 to 216 (45 in longest run, maxScore = 52), zone=40-143. (CA)12 154 to 177. Sequence is full of multiple minisatellites - too difficult to design primers. Bases 27 to 39 show homology to 25a9 bases 257 to 269 (complement) (13 in longest run, maxScore = 33).
PCR Comments : 


Length of sequence : 427  Consensus sequence is :

..

TCGATGGTCT GCTTGCAACT GGGGACCCTC GACAAACACT CACACCCCTA 
GTTATGAGTC GATGGTCATC ATGCAACTGG GGACCCTCGA CAAACACACA 
CACCCTCTAG TTGTGAGTCG ATGGTCGGCT CGCAACTGAG GACCCTCGAC 
AAACACACAC ACACACACAC ACACACACCC TTAGTTGCGA GTCGATGGTC 
GACATGCAAC TGGGGACCCA CGACAAAACA AACACACACA CACCCTAGTT 
GCAAGTCGAT GGTCATCATG CAACTGGGGA CCATGACAAA CACACAAAAA 
CCACCCTTAG TTGTGAGTCG ATGGTCGACA TGAAACTGGG GAGCCTCGAC 
AAAACATAGA CTCACCTAGT TGTGAGTCGA TGGTCTGCTT GCAAACGGGG 
GCCCCCAACA AACACACACA CCCTTAG
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wmc(12e2) Plate reference : 12e2
WMC locus number : WMC Length of sequence : 265
Last updated : Tue, Dec 30, 1997 at 10:11 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 38 to 58 (complement) show homology to 8c12 bases 285 to 305 (21 in longest run, maxScore = 78). No substantial microsatellite.
PCR Comments : 


Length of sequence : 265  Consensus sequence is :

..

CACCCACACA ACCACACACA CACACACTTC CCAACACCGA AGGAGTAGGG 
TGACACCTCG ATCTTGATAC TAATCTATCG ACTGGCTTCT CTCTCAAACA 
CACATACACG CGCACACACA CACACACTAC CTGACACCGA AGGAGTAGGG 
AGGCACCTCT ATCTTGATAG TAATCTATCT ACTCCTCTTT AAAACACACA 
CACATACACA CATACTCGCC AGTTGTGCCT CTGTGCCTAT CGCGCACACT 
CTCGATACGT CTTTC
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wmc(12f2) Plate reference : 12f2
WMC locus number : WMC Length of sequence : 357
Last updated : Jeu 27 Aož 1998 at 12:04
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 21 to 37 (complement) show homology to 7b8 bases 287 to 303 (17 in longest run, maxScore = 63). No substantial microsatellite. Bases 100 to 131 show homology to 10h7.1 bases 182 to 213 (complement) (32 in longest run, maxScore = 73).
PCR Comments : 


Length of sequence : 357  Consensus sequence is :

..

GGGACCTGCG CGAGCTGTAC GCCATGTCGG AGAAGTCAGA GGGGCCAGCA 
GAGAAGAACT GGACGCCAAT TGCAGCACCC ATCGGCACGG GGCCAATGTC 
ACCAACGGTG GTCGGAGTAG ACAAAGTGGT CGGCGTAGAT GACGGCAACA 
CTGGGGACTG GTTGGTGCTG GACGAAGAAG AAGGAGGTGG ATGAGCAGCT 
GCGACGGCTA TGGGAGTAGG ATCGGAGGCG GCCAAATAAG AGCGGCAGCG 
GCGGCCTGAC GGCGCGGTGG CGGCTATGTT AAGAGTGCGG CGGCGGTGCT 
CGAAGTAGGC GAGGAACCCT CACAGGATGA CGAAGACCGA CGCAGACGGT 
GGCGTAC
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wmc(12g2) Plate reference : 12g2
WMC locus number : WMC Length of sequence : 282
Last updated : Lun 5 juil 1999 at 17:23
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite. Bases 126 to 136 show homology to 26g10 bases 126 to 136 (11 in longest run, maxScore = 20).
PCR Comments : 


Length of sequence : 282  Consensus sequence is :

..

CATCCCTCCC CAATCCCTAG CTCCTCTCCC CTTCTCCACC CACAGCCGCC 
GCCGCCGCAC GCCCGCAGCC CTCCTCCGCT CCGCTCTGAC CATCCAAATG 
GTGCTCCTCC TCACGCCCAC GCCGTCCTCC TCCTCAAGCC ATCACCGTCG 
TCTCCCCTCC TCCGTGACCG GCCTGCGTGA TCCCAACGCG GGGGGACGAC 
ATCAGCCATG GAGCGGAGCC CTGTGGGACG AGGTGGAGAT TGGAGAATGC 
CGCGGCGATG AGGGGCACCA CGTACGTGGT AC
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wmc(12h2) Plate reference : 12h2
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Dec 30, 1997 at 10:11 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : No sequence 19.12.97.
PCR Comments :
No sequence available for this clone
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wmc(12a3) Plate reference : 12a3
WMC locus number : WMC Length of sequence : 269
Last updated : Tue, Dec 30, 1997 at 10:11 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments : 


Length of sequence : 269  Consensus sequence is :

..

GCCGGGTGGC GGCGGCGGCG GAGATGAGGA GGAACGCCAC AAGAGGTTGT 
TCATGTTCTT GATCTCTTCC TTGTGATCAT TGCCGCCTCT GCATGGGGTG 
TCGTTCATCA ACTCCACCGA TGCAACAACT ACTTGCATAG AAAATATAGA 
AAACAAAAAT TTTGTGTTTT GAGTGGACTT GAATTTGAAA TTGTAGCCGG 
TGTTTAAAAC CATTAAAGCG AACATATTTA ATAAGGCCAT GAGAAGACAT 
CTGTAAAACA CTATAGTAC
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wmc334(12b3) Plate reference : 12b3
WMC locus number : WMC334 Length of sequence : 220
Last updated : Mar 17 Nov 1998 at 10:55
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 5' end. (CA)5G(CA)6 at 162 to 185.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
cTcgAAgcAggATgcATTTA at 89 length 20 bases, Tm 61
Reverse primer details :
TcAAATTTTgggATAgcggg at 186 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 117 bases.


Length of sequence : 220  Consensus sequence is :

..

GTAAGTAGCT TAACTTGGCG GATGCACCAG TGTCAAGCTA GAAAGCACAG 
CACTGACATG GCGTGCATCC ATGTATGGAG TATCAGCCCT CGAAGCAGGA 
TGCATTTATT TAATCAGAGA GGCGCGGGCC CTACCGCGAT GGCCAGCGAA 
ACCCCACAAT CCACACACAC ACGCACACAC ACACACCCGC TATCCCAAAA 
TTTGATGCCG GGGTTCGTAC
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wmc(12c3) Plate reference : 12c3
WMC locus number : WMC Length of sequence : 165
Last updated : Tue, Dec 30, 1997 at 10:11 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 163 show homology to 11a3 bases 1 to 163 (163 in longest run, maxScore = 137). No substantial microsatellite.
PCR Comments : 


Length of sequence : 165  Consensus sequence is :

..

ATATGCATTG CTGATGCTCA GTAAAAACTT ATATAGTTGA CACAATCATA 
TAGAACACCA TAGCACACTG AACTTGCATA CAAAAATTTC ACGCAGGCGG 
AACAATCAGG ATGGAAGCAG TGGATCGCTA CGGGTAGGGC TATGTTGAAA 
CCAACGAACT CGTAC
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wmc(12d3) Plate reference : 12d3
WMC locus number : WMC Length of sequence : 98
Last updated : Tue, Dec 30, 1997 at 10:11 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : No insert.
PCR Comments : 


Length of sequence : 98  Consensus sequence is :

..

GTTGTAAAAC GACGGCCAGT GAATTCCTCG AGGCGCGCCT CGAGGGATCC 
TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT GGCGTAATCA TGGTCATA
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wmc(12e3) Plate reference : 12e3
WMC locus number : WMC Length of sequence : 98
Last updated : Tue, Dec 30, 1997 at 10:12 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : No insert.
PCR Comments : 


Length of sequence : 98  Consensus sequence is :

..

GTTGTAAAAC GACGGCCAGT GAATTCCTCG AGGCGCGCCT CGAGGGATCC 
TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT GGCGTAATCA TGGTCATA
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wmc(12f3) Plate reference : 12f3
WMC locus number : WMC Length of sequence : 221
Last updated : Mon, Mar 16, 1998 at 2:56 pm
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 59 to 119 show homology to 3a9 bases 557 to 617 (61 in longest run, maxScore = 94). (CA)13 182 to 207. No room for primers.
PCR Comments : 


Length of sequence : 221  Consensus sequence is :

..

GCCCCCCCCA CACACACACC CACACTTCCC AACACCGAAG GGAGTAGGGT 
GACACCTTGA TCTTGATACT AATCTATCGA CTGGCTTCTC TCTCAAACAT 
ACACACACAC ACACTACCCA GCACCGAAGG AGTAGGGTGG CACATCAATC 
TTGATAATAG TCTATCTACT CCTCTTTCAA ACACACACAC ACACACACAC 
ACACACACTC GCTAGTTGTA C
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wmc335(12g3) Plate reference : 12g3
WMC locus number : WMC335 Length of sequence : 210
Last updated : Mar 17 Nov 1998 at 10:55
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 11 to 30 show homology to 9f4 bases 305 to 324 (20 in longest run, maxScore = 20), zone is 1-45. (CA)5G(CA)12 88 to 113.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide;
Forward primer details :
TgcggAgTAgTTcTTccccc at 36 length 20 bases, Tm 65
Reverse primer details :
AcATcTTggTgAgATgcccT at 131 length 20 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 115 bases.


Length of sequence : 210  Consensus sequence is :

..

GGCATGCCCG CCCCACGGAG GTTCACCTAG TAAAGTGCGG AGTAGTTCTT 
CCCCCATCAC ACACACACGC ACTCACTCAC ACACACACGC ACACACACAC 
ACACACACAC ACACTTTCAC TCAGCTAGAG AGGGCATCTC ACCAAGATGT 
ATCAAAACGG ACATACACGT AGAACCTGAT AAGGCAGGAG TAAATGTTGA 
TTTCTTGTAC
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