Wheat microsatellite WMC 25/06/01

wmc(12h3) Plate reference : 12h3
WMC locus number : WMC Length of sequence : 394
Last updated : Tue, Dec 30, 1997 at 10:12 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 193 to 261 (complement) show homology to 5a6 bases 72 to 140 (69 in longest run, maxScore = 153). No substantial microsatellite.
PCR Comments :



Length of sequence : 394  Consensus sequence is :

..

GTACTACCAT GTCCATATAA TTGAGTTACG ATGAGATACC GTGTAAACAG 
CCGCTCCGCG GATATCCGTC AAAAAGAAAA AACAGCCGCT CTGCATATAG 
AGATAGCGGC GGCGGCCGAG GCAGCGGCTA CCGGAGAAGA GGTCAATCGT 
CGGTCGGTGG GCGGTTGCGG CGTCATAGGA GGTCAATCCT TAGTCTGCGG 
CGGGAGATAG GAGGAGCTCA ACCATCGAGG TCGGCGGCGG CGTGATTCGA 
GCACATCCAT CACGGTCGAT GGCGGGATAG TGGAGGTCGA ACTTCAGGTG 
GCAGCCGCAC TAAGCTTTGC TCCTCGACCC TGCTATCTCG GCGTGCCGCC 
GCATCGCGGT TGTCTGTCTG CTGGGTGGAG GTCGTCGTGC GGCT

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wmc336(12a4) Plate reference : 12a4
WMC locus number : WMC336 Length of sequence : 461
Last updated : Mar 17 Nov 1998 at 10:55
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GCC)7 165 to 185.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
gTcTTAccccgcgATcTgc at 102 length 19 bases, Tm 65
Reverse primer details :
gcggccTgAgcTTcTTgAg at 196 length 19 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 113 bases.



Length of sequence : 461  Consensus sequence is :

..

CGGGAGGAGT TCATGGAGAT TCTATCCATA TTGGAACGGG TTGTCGGGTT 
CCACTGTCAG AGACAACGGA ACATCGAACG AAAGAAGATA AAGTTGATGG 
AGTCTTACCC CGCGATCTGC TTCCCGGCCT CCGCCGCCCC TGCCACTATC 
CTCTCGGCTC AGCTGCCGCC GCCGCCGCCG CCGCCGGTTG GGAACCTCAA 
GAAGCTCAGG CCGCGGGCTG GTCGCCTGGG TGGCAGAGAG TGGGGGCTAG 
GGAAAGCAGA GGAATGGCAG GCGGGAGGGC GGTGGAGGGG TGGAGCTGCT 
GGCATGGCCG GAGCGGAGCT TTGTAGATTT GGGGATATCT TGCTTGGTTG 
GTGCTTGGAG AGCAATGGCT TGACCTGAAC CCGGTCGTGG GCGTGGGTCG 
TCGTGGTGGC GACCTGCGGC GCCCTTTCCT GCCGAATTCC ATTCAACCTT 
GCTACACGTA C

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wmc(12b4) Plate reference : 12b4
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Dec 30, 1997 at 10:12 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : No sequence 19.12.97.
PCR Comments :
No sequence available for this clone
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wmc(12c4) Plate reference : 12c4
WMC locus number : WMC Length of sequence : 161
Last updated : Tue, Dec 30, 1997 at 10:12 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 161  Consensus sequence is :

..

GTACGAGGAA GACGAGGTGG CGTCCACCGT TGACAAGATA CTTCCCGTCG 
ATGATCCAAC GAGAGTCGGG CTTCATGTGG GGGAGATTAC GGAGATGCTG 
CGCCGGGTAG TTCACCGGCG AACGGCGGCG GCGGCGGTCC GGCCCTGGAA 
GGGGCCCTTG C

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wmc337(12d4) Plate reference : 12d4
WMC locus number : WMC337 Length of sequence : 241
Last updated : Mar 17 Nov 1998 at 10:55
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 80 to 134 show homology to 1B4 bases 156 to 210 (55 in longest run, maxScore = 51), zone 79-241 Reverse primer has 4 mismatches to 1B4 sequence. (GT)33 65 to 130.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
cgTcTAggATTTTAcAAAccAc at 43 length 22 bases, Tm 57
Reverse primer details :
gAcTTAAcccATTTAgcTTcAT at 133 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 112 bases.



Length of sequence : 241  Consensus sequence is :

..

GTGTCCACAG TCAAGGACAG TTGGGCGGTG CTGCTTAAAC TACGTCTAGG 
ATTTTACAAA CCACGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT TGATGAAGCT AAATGGGTTA 
AGTCAGATGA CTTGACACGA TGATCAAGTT GGATTGGTGT CCAACTCTGT 
TTATGTTGAT ATATGTAACT TGTTCATGCG GATATATGTA C

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wmc(12e4) Plate reference : 12e4
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Dec 30, 1997 at 10:12 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : No sequence 19.12.97.
PCR Comments :
No sequence available for this clone
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wmc(12f4) Plate reference : 12f4
WMC locus number : WMC Length of sequence : 195
Last updated : Wed, Mar 18, 1998 at 3:34 pm
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)8 13 to 28. No room for primers.
PCR Comments :



Length of sequence : 195  Consensus sequence is :

..

GTACCACTAC ACCACACACA CACACACACG CTCGGGCACG CACACACACG 
CACTAACTAC CACTACGACT CACATGGCGC CAGGACTCAG CTGGTCCACT 
CACCACGGCC GCATGCACGC CATGCACTAA CAACCCACAA GCATCGGATC 
AGCTTAAGCC ATGTCCTGCA AGTCAAGATT AAGGCTAACA TTGTC

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wmc338(12g4) Plate reference : 12g4
WMC locus number : WMC338 Length of sequence : 301
Last updated : Mar 17 Nov 1998 at 10:55
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)22 185 to 228.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
AgATccgTATgATATgcTTgcg at 148 length 22 bases, Tm 63
Reverse primer details :
cTTcATcAgccTccATgAcAcA at 262 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 136 bases.



Length of sequence : 301  Consensus sequence is :

..

TGTAGGATCG ATTTGATTGA TATGTTTAGT GTAGAATCGA TCTGATTGAT 
ATGTTTAGTG TAGAATCTGA TGATATATGT GAATTGAAGT GTATGCTTTT 
TTGATAATTG TGCATGACAT GCTCATTTAT CACGTGGATT TGTTTTTAGA 
TCCGTATGAT ATGCTTGCGA GTGTGTGTGT GTGAGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTTT TCAGAAATTG CCTAAGAGGC 
TAGCTCCTAG TTGTGTCATG GAGGCTGATG AAGCAGGCTA TGCTGGAGTA 
C

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wmc(12h4) Plate reference : 12h4
WMC locus number : WMC Length of sequence : 462
Last updated : Mar 25 Aož 1998 at 11:25
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' and and to Rsa1 at 3' end. Bases 1 to 462 show homology to 12f7 bases 1 to 462 (complement) (462 in longest run, maxScore = 391), true duplicate, 12h4 has precedence. No substantial microsatellite.
PCR Comments :



Length of sequence : 462  Consensus sequence is :

..

CACGAGGATC GGAAGTGATT AACCGCAGTG TGATATGATA TGGCTGGGGA 
AAGCGGGGCG GAAAACCTGT TGACGGCGGC AACGGTGTGC GGGCGTGTCA 
AGAGATAGCG ACGTGACACG TGACCAAAGA AGCTCGACAG GGATGGCAAC 
ATGGCGGCAG GCGGCGGCGC GTGGCCGTCA AAGAATCTCG ACGAGGAGGT 
GGCGAATTAC CTCCTGTTTG TTGGGTCGAT TGACCGAACA CTTGGTGCGC 
AGGTGGCCGC CAGATCTCAA GAAGGCGATG GATGTCGCAT CAGGAGGCTG 
CAATGGCAAG TGGCGCTGGG GTTGGGTCTG GTCCGGGAGT AGCGCCGCCC 
GAGGTCGGGG TGGGAGGTGA GGTCGCCTCA CCGTCGGGGC CTCCGCTTCC 
CCACGTCCTC TTTCCCGAGG AGGAGCACAT CGTTGGCAGC AACCACGCTG 
AAAGCACGGT AC

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wmc(12a5) Plate reference : 12a5
WMC locus number : WMC Length of sequence : 258
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end, 3' end not located. Bases 192 to 258 show homology to 11c4 bases 288 to 354 (67 in longest run, maxScore = 58). No substantial microsatellite (small CA repeats). Bases 216 to 235 show homology to 20b12 bases 221 to 240 (20 in longest run, maxScore = 21).
PCR Comments :



Length of sequence : 258  Consensus sequence is :

..

CTCACACACA CACATACACA CACACACACA TACACACACG CATACACACA 
TACACACACA CACTACACGT ACAGTAGCAG CCACACGCAT GTGAACGGGT 
GGCGTCCAAA TGGAATGCCC AATGGTCTTC ACAACCTCAA ACGCAGCAGT 
GTGATCAACG ATGGCCTGAA TGAGTGGAGC ATATGGGAAC TGACGCGCCT 
CGAGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT GGCGTAATCA 
TGGTCATA

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wmc339(12b5) Plate reference : 12b5
WMC locus number : WMC339 Length of sequence : 416
Last updated : Mar 17 Nov 1998 at 10:56
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)8 118 to 133.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
ccgcTcgccTTcTTccAg at 29 length 18 bases, Tm 66
Reverse primer details :
TccggAAcATgccgATAc at 215 length 18 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 204 bases.



Length of sequence : 416  Consensus sequence is :

..

GTACAAGCAG AGCAGGCACC GCCTGCTGCC GCTCGCCTTC TTCCAGCTCC 
GCCGCCTGCC GGTTCCCGCT GCCGCCGCTC ACCTCCTGCC GCCGCCGCCT 
GCCTGTTCCT GTCGGATGAG AGAGAGAGAG AGACGAGAGA GGGAGATAAG 
CGGGCCGTCG AGGGAGACGC GAGGGGTGAG CACGAGCCGT TCCGCGTCGT 
TCCTCCGATT TGGAGGTATC GGCATGTTCC GGATATAGAC CGAATATTCG 
CTCGGTGGGA ATGATGTGGT TCCGGTTCCA GTTACATACC AAACGTAGGA 
ACAGGGCCCA GAAACGGGTC CGACCCATGA CATTCCAGCC CGTGACTCGA 
ACCAAACACA TCCTAAATGT TGCGAGTTGA GACGTGTGTG AAGCTGATAT 
CTTTTGCACC TTTGCC

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wmc(12c5) Plate reference : 12c5
WMC locus number : WMC Length of sequence : 269
Last updated : Tue, Dec 30, 1997 at 10:13 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 269  Consensus sequence is :

..

GCGGAGCAGG TGCTCTATAG CCTTCTTCGA GATGACAATG CCGAAGAGGT 
CGAGCTTCTT GAGTTGAGGA AGAAGAAGGG CGGGCGCGGC ATTAATCTGG 
GGAAGATAGT AGGAGCTGAA GCTGGCGCGG CGCAGCGTTG GCGTGAGGCG 
GAGTGCGGAC GGCGGCAGAG AGCGACATCG TCCAGCTTCA AAGCTGAGCT 
CCTCGAGCTG ATCTAGGGCG GCGGATAAGA ACCACTCGTC GAACTTGGGT 
TGGACCTTGC AGTTGGTAC

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wmc(12d5) Plate reference : 12d5
WMC locus number : WMC Length of sequence : 252
Last updated : Mon, Mar 16, 1998 at 3:07 pm
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (CA)9 26 to 43. No room for primers.
PCR Comments :



Length of sequence : 252  Consensus sequence is :

..

GTACACACAC ACGCATACAC GCATACACAC ACACACACAC ACACTACACA 
CACAGAGACA CACACACACA CGTGCATACA CACACACTAC ACACACtCGC 
ATACACACAC AGACACACAC AAACACACAC ACACACTACA CGTACCACCT 
AGGCTCCTTC TTTTGAGGTG AAAATTTCTG AAGACGGTCT TCTTAGTAAC 
TGATCATCCT CAGTCAAAAC TTACGCCCAT TAGCCATGTG CATTTCCCGT 
AC

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wmc340(12e5) Plate reference : 12e5
WMC locus number : WMC340 Length of sequence : 263
Last updated : Lun 30 oct 2000 at 16:59
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 161 to 184 (complement) show homology to 6c1.1 bases 79 to 102 (24 in longest run, maxScore = 53), zone is 160-252. Primers do not overlap this zone. (GT)14 57 to 84, (CT)16 21 to 52. Bases 213 to 263 show homology to TW6a02.2 bases 84 to 134 (complement) (51 in longest run, maxScore = 83).
PCR Comments : 51C anneal, product.
Forward primer details :
gAggAgTTcAcTcAcccTTcT at 2 length 21 bases, Tm 59
Reverse primer details :
gTTgggATcggATcgAgTA at 90 length 19 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 107 bases.



Length of sequence : 263  Consensus sequence is :

..

GGAGGAGTTC ACTCACCCTT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTGTCTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGATGCT ACTCGATCCG 
ATCCCAACAT GTTTCTGGAT TGGCCGATTG CTTCTAGATT CTCCCCCCTC 
TCTACCCTAG GGGTGGCTAG TCCTATTTAT AGGCAAAGGC CCTGGGCCTC 
TTCCCAAATA TAGAGCGGGA AGGGCGCCAA CAATTGGCCA TTTTGAAGGG 
GAACATCTGG TAC

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wmc341(12f5) Plate reference : 12f5
WMC locus number : WMC341 Length of sequence : 254
Last updated : Mar 17 Nov 1998 at 10:57
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)24 55 to 102.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
AcATgggcTAcATgAgAgAAgA at 21 length 22 bases, Tm 61
Reverse primer details :
AgAgTggcTcccTTTTcAccTA at 119 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 120 bases.



Length of sequence : 254  Consensus sequence is :

..

GTACAAACAA ACCGAAGTTT ACATGGGCTA CATGAGAGAA GAAGGAAAAG 
AAAACACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CACCAAACAA CACATAAGTA GGTGAAAAGG GAGCCACTCT GAGAACCTTA 
GAGACGATGG ATGAGAAAGC TCATCACGAC CCAAGCACGG CAATCATAGG 
GTAGAGCTCA CAGGACATGA TAACGGGAGG GACAACCTAG CTCGAAGCAT 
CGCC

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wmc342(12g5) Plate reference : 12g5
WMC locus number : WMC342 Length of sequence : 254
Last updated : Mar 17 Nov 1998 at 10:57
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)13 78 to 103.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
AgATgAgATggcAAgcTcAAgA at 19 length 22 bases, Tm 63
Reverse primer details :
gAAAgcTTcgcATcccAATAgT at 212 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 215 bases.



Length of sequence : 254  Consensus sequence is :

..

GTACATACTC TACATTGGAG ATGAGATGGC AAGCTCAAGA TTTGTTTTAG 
GTGGCTTGAA AAATCTAAGT GCTTGATGTG TGTGTGTGTG TGTGTGTGTG 
TGTAACATGT ACGGGCTCTC TACTACTAGA AGTTGTGTTC GGCTCATCCG 
ACAACTTCCG TCGCGAAAAG TTAGTCTTCC ACATCGCCCC ATTTAAAAGT 
AGCTATCAAG CACTATTGGG ATGCGAAGCT TTCGCCTGCT TTAACGCAAT 
ACCG

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wmc(12h5.1) Plate reference : 12h5.1
WMC locus number : WMC Length of sequence : 218
Last updated : Tue, Dec 30, 1997 at 10:14 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts (2) in this clone. This is the first insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 218  Consensus sequence is :

..

GTACATGTCA CTAAGGGGAT TGGGCAGCGA CTCTTCCTCT CGTCTCGCCT 
CCGAGCTAGG TTTAGCGCCG CCGCCGCCGC CTCCATACCT GCTGCTCTGT 
TGTTGCGCTC CGGAGGAAGG GCCCCTGTTT AGACCCGCTC CTTCCCTAAG 
TTAGGCCGTC TGCACGTCGT CATCGTCCTC GTCGGTTGTC GTCAGAAACA 
CCGGAATACG TGAGAGTG

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wmc343(12h5.2) Plate reference : 12h5.2
WMC locus number : WMC343 Length of sequence : 624
Last updated : Mar 17 Nov 1998 at 10:57
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts (2) in this clone. This is the second insert. Trimmed to insert at both ends. Bases 493 to 562 show homology to 13g1.2 bases 406 to 475 (70 in longest run, maxScore = 105), zone is 381-567. Reverse primer has 6 mismatches to 13G1.2, forward primer is outside of this zone. (GT)8 326 to 341, (GT)31 346 to 407.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
cTTTcccAccccAcTAgATg at 262 length 20 bases, Tm 61
Reverse primer details :
ccAccgTTAgTggccTATgc at 476 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 234 bases.



Length of sequence : 624  Consensus sequence is :

..

ATCCGTGCCC TCACCTCTTC CGTCAAGATG GACACCACCG TGCCAAGGCA 
TAGGTCCGAG TCATCGCATC GGTCTTTGTC CCCTCCTCCA TCCACAACAC 
ATGACGTGAC GGGCGGCCCG GTGTGCCGTG TCTGTGTCTC GTGCTTCGAC 
GCTCCGGTGA AGCACATCAC ATCCATGTTG ACACGTCCAC ATATTTTTGG 
GCCGACAATT TCACATGTAG AAAGTGTTAT GCATTCGACA ATTTATTTAT 
GTTTTGCAAT TCTTTCCCAC CCCACTAGAT GGCATCTTGT TCATAACACA 
AAACAAGACT TTGTGCACAT CCGGGGTGTG TGTGTGTGTG TGCGCGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTCGC AGGGTCCTCG GTGGCGGATG TAATTTAAGT GTGCATGGCT 
TCATCATAGA GAGGTGATGT GTATGGCATA GGCCACTAAC GGTGGGGTGC 
GAGAGAGAAA ATGCCCGTAG AGAGGGAAGA GAAGGTGTGT GCGCGTGAGA 
GGAGTCGACA TCAAGAGAAA GTGTTTGTTC AAGAGACACT AAGGAAGACT 
ACTATATATC GATGGTGCAT GTAG

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wmc344(12a6) Plate reference : 12a6
WMC locus number : WMC344 Length of sequence : 326
Last updated : Mar 17 Nov 1998 at 10:57
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)16 189 to 220.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide (multi-locus polymorphic)
Forward primer details :
ATTTcAgTcTAATTAgcgTTgg at 80 length 22 bases, Tm 57
Reverse primer details :
AAcAAAgAAcATAATTAAcccc at 304 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 246 bases.



Length of sequence : 326  Consensus sequence is :

..

GTACGGTCAG TAGTTGACTA GTCAACAGGG TCAAAAATGG ACCCATATGG 
CATACACTCA TGTGTTAAAC GGTTGTTTTA TTTCAGTCTA ATTAGCGTTG 
GCTGCCCACA GTCATATGCA TGTTAGTCTT AGTAATTAAA CTAACCTAAA 
CAACGGGGCC ACATGGCAGT GTGCCACTAC TAACCTAACA CACACACACA 
CACACACACA CACACACACA CTAATTACAC CGGGCCGGCC ACACACAGTC 
GTGGCCAAGC CGGCCACACA CACACCACAC ACAGCATGCA ACACACTATA 
CTAGGGGTTA ATTATGTTCT TTGTTA

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wmc(12b6) Plate reference : 12b6
WMC locus number : WMC Length of sequence : 338
Last updated : Tue, Dec 30, 1997 at 10:14 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 120 to 161 (complement) show homology to 12a2 bases 206 to 247 (42 in longest run, maxScore = 145). No substantial microsatellite.
PCR Comments :



Length of sequence : 338  Consensus sequence is :

..

GTACTCCGTG GGCGCCAAAA TACCCTCTCC ACCCCACCCT CCTGTGTCCG 
CCGCGCCGTC CGCACCCCAT CCACCGCCGT CCTCCTCCAC CATGCCGGAG 
CTGATACCCT GACACCGCTG TCCATTACAA GAGATCGACC TCTCTCATCC 
ACGGCTTCGG ACCGGGATCC TTGCATCCTG TCCTCTCGCT TCATCCCTGT 
CGTCGTCCAC CTTGCCGGCG CCACTCCTAC GACCGTCACG TCCACCGCGC 
CCCGCCGCCA CCGTCTACCC TCCTAGATCT TCTTCCACGC CGTCGACAGC 
CATCCTACCG CAGCACCGTC AAATTCTTAG CAGATGCG

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wmc345(12c6) Plate reference : 12c6
WMC locus number : WMC345 Length of sequence : 385
Last updated : Mar 17 Nov 1998 at 10:58
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 207 to 232 (complement) show homology to 6d8 bases 196 to 221 (26 in longest run, maxScore = 69), zone is 198-322. Primers are not within area of homology. 12b7 is a duplicate, 12c6 has precedence. (GT)21 106 to 147.
PCR Comments : tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide (several loci).
Forward primer details :
AcTAcgTccTgATATTTccgcA at 76 length 22 bases, Tm 62
Reverse primer details :
AgcAAcAccTTTcTcATcAccA at 157 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 103 bases.



Length of sequence : 385  Consensus sequence is :

..

GTACTACCTC TGCAGAGTGT AAACTATTCG AATAGCCGTG TCCACGGTCA 
AGGACAGTTG GGTAATCCTG CTTAAACTAC GTCCTGATAT TTCCGCATAA 
ACCAAGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGCG 
TGTGTGTGGT GATGAGAAAG GTGTTGCTAT GATAGCAATG ATATGACCAA 
GTTTGGTGAC TTGGCATATA GGGTGAACCC GGATGGTTCA GCCCTCGGCA 
GATATATTGA TATATGTAAC CTGTTCTTCA AGAGTAATTC TTTAACTTGA 
TGCATATTCA TGATCATTCC ACCCAGGTGA GTTAAACTAG AGATGCATGA 
TATTGTTATC CTTCACTTTC GTTGATTATA TCATG

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wmc(12d6) Plate reference : 12d6
WMC locus number : WMC Length of sequence : 303
Last updated : Tue, Dec 30, 1997 at 10:15 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 303  Consensus sequence is :

..

GTACCAGCCC TGGCCGGCGA TGTTGGGGAC GACCTGCATC TTCCAGAGCA 
GATAGTTGGT GTGATCTAAA CGCACCGTGG TGGTGTTGAG GTTCTGGCCG 
GTAGGGGCGG AGACGATGGC GCCGTCGGGG ATGGATGGTT TGGAGGACTG 
GGACGAGGTG CTCGAAGTGG AGAGGGCGAG CTGCAATGCG GCGGCGGTGT 
CACCCATCGC GTCGAGGTGC GGCGGCGGCG GCGGCAGCGG GGAAAAGAGA 
CGGCGGCGGC GGAAGCGCGT GAGCGAAACC CTAGGTCGGA TCGACCCGCT 
CTG

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wmc(12e6.1) Plate reference : 12e6.1
WMC locus number : WMC Length of sequence : 328
Last updated : Mon, Mar 16, 1998 at 3:22 pm
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts (2) in this clone. This is the first insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Duplicate of 6h12 bases 1-328. (GT)8 31 to 46, (GT)12 49 to 72.
PCR Comments :



Length of sequence : 328  Consensus sequence is :

..

GTACTGCCGG GTTTGGACCA ACCTACAGTA GTGTGTGTGT GTGTGTATGT 
GTGTGTGTGT GTGTGTGTGT GTTGATTTTA GGAAATTAAA CAAATATACA 
AAGAAGGATC ACACCCATTT CTATTTATAA ATGACATATT GGAGAAATTG 
GCTAAAATTC TTCTTTTTGT TACCTCGATG AGTTGCCATC TCCTTAGTTG 
ACACATCATA TATGCAATTG CCCTCTACTC ACTTCCTTAG TATATAAATC 
ATATATTTGA ATTATGTCAT GCCGTGATGT TTACATAGAT AGCATGTATC 
TGAATTATGG CATGCCAACC CATTTTCT

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wmc(12e6.2) Plate reference : 12e6.2
WMC locus number : WMC Length of sequence : 294
Last updated : Mon, Mar 16, 1998 at 3:23 pm
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts (2) in this clone. This is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Duplicate of 6h12 bases 347 to 638.
PCR Comments :



Length of sequence : 294  Consensus sequence is :

..

GTACAAACTG TTAGCTTACA CCTGATTGTA GTTAAAGAGT TGTTTGTGCA 
ATGTGGATGC ATGCACTTGT GTGATTGTGG GCTACAACTT GCTGCAAAGT 
TGACGGCAAT TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTT GCAGTAAAAA TGAGGCTGCT GAGAAGCTTG TAGAAAATTA 
GAGTTGTAGC ATTCGTTGCT CAGCTCAGTT GGAAACTTGC AGTATCTGTT 
ACAGTTGAAA AGAAGAAAAG AGAAACGGCA TCCGTCGCCT GAAA

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wmc(12f6.1) Plate reference : 12f6.1
WMC locus number : WMC Length of sequence : 264
Last updated : Tue, Dec 30, 1997 at 10:16 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts (2) in this clone. This is the first insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 264  Consensus sequence is :

..

TCGCCTCTGA GTTCGCCATC GCCTCCATCC GGCTTGGCTG GGTCTTGCCC 
TCCCCGACCG GTCCTCCTCT CCACGACTGG ATGGCATCCC CATCGGATGC 
TCATTCCGCC ACTAGCGGCG GCGGCGCGGC GGCTGCTAAA CAAGACCTTG 
GCGATATCTT CAATCAACTG GATCTCAATG ACGATAACTT TGATGATGTC 
GAGATTGATG AGGAAGATCC AGAGATCAAT GAAAGCGTCA GGTGGCTTGC 
CCTAGCGAGG GTAC

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wmc(12f6.2) Plate reference : 12f6.2
WMC locus number : WMC Length of sequence : 380
Last updated : Mon, Mar 16, 1998 at 3:25 pm
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts (2) in this clone. This is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 341 to 357 show homology to 17e11 bases 249 to 265 (17 in longest run, maxScore = 79), zone is 93-279. (GT)14 330 to 357. No room for primers.
PCR Comments :



Length of sequence : 380  Consensus sequence is :

..

GTGGCTGGTC AGGTTTGGTT GTGACTCTCA ATGCCTTTTG GCTTCATTAA 
TTTAAAGTCG GGCCTCGGCC TTTTCTCTAA AAAGTTGAAT TCAAATATTT 
GAATAAGAGA TCATGATGTT TGAAACCCAA GCATGCATGA ATATAATTGA 
GATATGTGTG GTGTGGTTTT GAAACGAGCA TGCTATACCG TATACACATT 
GAACAAGGGC AAACTGATTT GAATTTGGGA TATCGATGGC AGGCATCGTT 
TGGCCACATT GCATCCATGC ATGAACACAA CTATTTAAAT TAGAGATGAT 
GAGTGGCTTT CCCAACTCAT ATCTAAATGG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTTGT GCATGTTAAA CACATTGTAC

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wmc346(12g6) Plate reference : 12g6
WMC locus number : WMC346 Length of sequence : 676
Last updated : Mar 17 Nov 1998 at 10:58
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. (GA)8 299 to 314, (GT)13 7 to 32,
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Forward primer details :
cTgAAgTTccAgccAAcAcA at 214 length 20 bases, Tm 62
Reverse primer details :
ATTcccTcATcccgTTgc at 400 length 17 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 203 bases.



Length of sequence : 676  Consensus sequence is :

..

GTACATGTGT GTGTGTGTGT GTGTGTGTGT GTTTGTGCAA CTGCACACAA 
AAAAAAAGGA GCCGGCTATG TTGGTAGCCA GAGCAAAAGA GAGAAAGGCA 
TTCATCGCCT GAAGAGAAAA CTGAACCTGT GTCTCGTCTG TAGTTTTTCT 
CTGGCTTTGC ATTTCGTGGA ACACCTCCTG AGCGTAGTGA GATCCACCAC 
AACAGAGCGT GAACTGAAGT TCCAGCCAAC ACAAACCTGT CAAAACCTGC 
ATACAGTGGC ATTGAGAGAG ACAACAGGGG AAGCAAGAAG GATTGCTAGA 
GAGAGAGAGA GAGAACGATC TGGAGAGAGA GAGAGATAGG ATTGCTAGAA 
AGACATGTCC CCCGCCATTG CACCGAATCG CCGGATGCGC GGAAGAGGGC 
AACGGGATGA GGGAATGGGG GCGGCGGCGG AAGTGCTGCA TTCACGCCTG 
CAACGACATT AAACATGGAG GAGCAGGGCC ATGCATGGGT AGTGGCGGGC 
GGCGCGGTTG CCTCTCGTGT TGGGGAGGCA GGATTCAGAG TGGCGGTTCT 
GTATCCCGTG ATTTGAGGAA ATGACACCTG AATGGGTGCA TGGGAAATTT 
CGTCTAGAAT TTTTTACTAG ATCCAAAATG CCACAGGATC TTCAGCTAGG 
AACTACTTAG TGGTTGCATC AGGTAC

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wmc(12h6.1) Plate reference : 12h6.1
WMC locus number : WMC Length of sequence : 317
Last updated : Mon, Mar 16, 1998 at 3:28 pm
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts in this clone. This is the first insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (Near) duplicate 11f6 . (GT)17 245 to 278.
PCR Comments :



Length of sequence : 317  Consensus sequence is :

..

GGTGAGGTTC CGGGCAAAGT CCCGCAGAGT GCAGAGGTCG TCGGCGTGGC 
ACGACGTCGC GCTCGTCGCC GGCAGGAGGA ACACCAAGCA GAGGAGCACT 
AGCCAGCATT TCGCCATGGA TTAAGCTGTG GTATGCTATG TATCCACAAA 
CAGAACAACT CTTTGTAAGA AACCTTCTTT GGGATGGAAG TAATTCACAA 
GTGCTCTTGG CAAAAACTGC TTGGGTGGTG CATGTTGTCT TATAGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGC GTGTGTTTGT AAGCATGCGT 
TACAAGTGGT TCAGTAC

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wmc(12h6.2) Plate reference : 12h6.2
WMC locus number : WMC Length of sequence : 327
Last updated : Tue, Dec 30, 1997 at 10:17 am
Owner : Weibull, Svaloff Weibull Date at Agrogene : 19.12.97 Public Domain : Yes
Comments : Multiple inserts in this clone. This is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 50 to 327 show homology to 11f6 bases 362 to 639 (278 in longest run, maxScore = 239). No substantial microsatellite.
PCR Comments :



Length of sequence : 327  Consensus sequence is :

..

GTACAGCGCA CGGAAACACT AGCGAGTCGA GCGAGCAGTC GTGGTTGGAA 
ACCCCAGAAG CAAAGCAAAG CAAAGCAAAC CCCTCAAACC CCACGCCGGA 
GAAACCAGAC AAACGTCGCA TGATTTTCCC CTCCCTCGCC GCCGCCGCCG 
CCTCATATGT GTAGAGCGCC TCCTCCGGAT CGCTACCTTA ATTCGCCGGA 
AATGATGGTG ACTAGTAAGT GTCAATTCTG ATGTGCTTGA TCTTCACACC 
GAAGTTGGTT GAAGCCCTCG AGGAAAATCG TTTGAAGACT TCATGCACTT 
CATGTTTGTA AGTGACAATG TGCACCC

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wmc(19a1) Plate reference : 19a1
WMC locus number : WMC Length of sequence : 136
Last updated : Thu, Mar 19, 1998 at 9:29 am
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 68 to 108 (complement) show homology to 9d1 bases 212 to 252 (41 in longest run, maxScore = 37). (CA)11 115 to 136. No room for primers.
PCR Comments :



Length of sequence : 136  Consensus sequence is :

..

CGGAAACGAG AGGATGCACC CACTCTCGCA GCTAAAATCG TAATACACTA 
CACAACATCT CTTTGTGGGA GTAGGTCGAT CTGTCGCTCT CTCTAACACA 
CACATGCTAT TAAACACACA CACACACACA CACACA

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wmc(19b1) Plate reference : 19b1
WMC locus number : WMC Length of sequence : 183
Last updated : Tue, Mar 3, 1998 at 12:08 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite (small GT and GC repeats).
PCR Comments :



Length of sequence : 183  Consensus sequence is :

..

GCGTGCGTGT GTGTTCATTG ATGGGGCGAA TTTGAAGTGC ATTGTGCGTG 
CGTGTGTGGT GTGCGTGCGT GTGTGTTCAT TGGTGGGGCG AATTTGAAGT 
GCATTGTGCG TGCGTGTGTG GTGTGCGTGC GTGTGTGTTC ATTGATGGGG 
CGAATTTGAA GTGCATTGTG CGTGCGTGTG TGG

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wmc347(19c1) Plate reference : 19c1
WMC locus number : WMC347 Length of sequence : 987
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Trimmed to insert at both ends. Bases 59 to 80 (complement) show homology to 7b1 bases 51 to 72 (22 in longest run, maxScore = 19), zone is 58-123. Primers are not in this area. (GT)9 806 to 823, plus small GT runs. Bases 26 to 56 show homology to 10h7.2 bases 88 to 118 (31 in longest run, maxScore = 39). Bases 37 to 80 show homology to 21b3 bases 166 to 209 (complement) (44 in longest run, maxScore = 35). Bases 12 to 43 show homology to 24f9 bases 2 to 33 (32 in longest run, maxScore = 28).
PCR Comments : tested 61C anneal, no product; tested 51C anneal, no product.
Forward primer details :
gATTTTTAccAAAATcATTAggggA at 719 length 25 bases, Tm 62
Reverse primer details :
cATggTggTAgTTTTATTTTgAAgA at 886 length 25 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 192 bases.



Length of sequence : 987  Consensus sequence is :

..

ATTTTTAACA TTCTAAAAAT AAACAAGATA GTTCTAATTC ATCTAAACAT 
TAGAAATTAA ACAGAAAATA AGTAAAAAAA TGTGTGTGTG TGTGTAGTGT 
GTGTGTATAT GTGTGTACAT GTGTAGTGCT GAGAGATCCC CTCATAATTT 
CCCCAAAGCG TAACCATGTG TGAATAAATT TTGAGCTAGT AGGGTTGCAG 
CCACGAGTAA GTCTTCCCTT GTTATTGTGT AGCCAGAATG CCGCAAAACT 
TCCATGCCTA AGCGAACTGT TGAGAGTACG TTTCGATTTC TGACTGTGTT 
AGCCTGGAAG TGCTTGTCCC AACCTTGTTT CTGAGCATGA ACGCCCGCAA 
GCCAACATGT TAGTTGAAGC ATCAGGGCGA TTAGCAGCAT GATATCAAAA 
CGCTCTGAGC TGCTCGTTCG GCTATGGCGT AGGCCTAGTC CGTAGGCAGG 
ACTTTTCAAG TCTCGGAAGG TTTCTTCAAT CTGCATTCGC TTCGAATAGA 
TATTAACAAG TTGTTG
(break)
GGTAAGAGTG AGAGTTTTAC AGTCAAGTAA TGCGTGGCAA GCCAACGTTA 
AGCTGTTGAG TCGTTTTAAG TGTAATTCGG GGCAGAATTG GTAAAGAGAG 
TCGTGTAAAA TATCGAGTTC GCACATCTTG TTGTCTGATT ATTGATTTTT 
CGCGAAACCA TTTGATCATA TGACAAGATG TGTATCCACC TTAACTTAAT 
GATTTTTACC AAAATCATTA GGGGATTCAT CAGTGTGTAG TGTGTGTGTG 
TGTTTGTGTG TGTCTGTGTG TATGTGTGTG TAGTGTGTGT GTGTGTGTGT 
AAAGCAAATT ACCATGCTAT TTAAGACTCT CAAAATAATC TAAGTGAAGC 
ATGGGAGTTC ATCTATTTCT TCAAAATAAA ACTACCACCA TGCTCTAAAA 
GATATAAGTG AAGCACTAGA GCAAATGACA AACTACTCCG AAAGATATAA 
GTGAAGATCA ATGAGTAGTA C

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wmc348(19d1) Plate reference : 19d1
WMC locus number : WMC348 Length of sequence : 422
Last updated : Mar 17 Nov 1998 at 11:01
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 273 to 309 show homology to 6b12 bases 55 to 91 (37 in longest run, maxScore = 90), zone is 231-417. (CT)12 29 to 52, Bases 148 to 215 show homology to 22f10 bases 396 to 463 (68 in longest run, maxScore = 119).
PCR Comments : 61C anneal, product.
Forward primer details :
AccccgAcTAccgTcTcc at 12 length 18 bases, Tm 62
Reverse primer details :
AATAcgAgTcggggTgcg at 135 length 18 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 141 bases.



Length of sequence : 422  Consensus sequence is :

..

CCGATCAGAC AACCCCGACT ACCGTCTCCT CTCTCTCTCT CTCTCTCTCT 
CTCACAGACA CACACACACA ACTCCTGCTT CCACTCCCCT TCCCGACTAC 
CGTCTCTCTC TCTCACACAC AAAACTCTCT CTTTCGCACC CCGACTCGTA 
TTTCTCTCAC AAACATTTAT CGACTAGCTA GCCTCTAGAT AGGTTTATAC 
ACCCGCACAC TCGTGCTTCC ACTATCGCAT ACATGTCTCT CTCCCGCTCC 
TTGTATCTAT GTAGATTTTT CTTCCCTTCT TGAGACACGC CCTCTCGATC 
ATGCACACAC ACACTATCGC CTCATTTTAA GTTGATACCT ATCGCACACA 
CACTCCTTGT GTCATTGTCA CACATGCACT CCGTCCTCCT CCACTCTCCC 
TCTCTCTCAC ACACACATGG GC

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wmc(19e1) Plate reference : 19e1
WMC locus number : WMC Length of sequence : 196
Last updated : Thu, Mar 19, 1998 at 10:02 am
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 42 (complement) show homology to 6d6 bases 48 to 89 (42 in longest run, maxScore = 26). (GT)24 50 to 97. No room for primers
PCR Comments :



Length of sequence : 196  Consensus sequence is :

..

CTAAAAAACA GAAAAATTTA TAACAAACAA ACTAATGTGT GTGTGTGTAG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTAGT 
GTGTGTGTGT GAGTGGACAT GACGGCCGGG GTGAGCTCAC CGACGAGGCG 
ACGATGGGCC GGCGACGACG GGGCGACGGG ACAGGGCAGC GACGAC

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wmc(19f1) Plate reference : 19f1
WMC locus number : WMC Length of sequence : 136
Last updated : Tue, Mar 3, 1998 at 12:09 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. NB 5' linker corrupted. Bases 19 to 88 show homology to 8h9 bases 43 to 112 (70 in longest run, maxScore = 52). No substantial microsatellite.
PCR Comments :



Length of sequence : 136  Consensus sequence is :

..

GAGAGAGAGA GACAGAGGGA GAGAGGAGCA TCCGTAACAC AACAACCATC 
TCTCTCGATT CGTCCGTCCC TCGCACGATC GCATGCAAAC ACAAGCATCA 
ACGCGCACAT TTTGACACCC TCACCCGGCC CCTGCC

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wmc(19g1) Plate reference : 19g1
WMC locus number : WMC Length of sequence : 191
Last updated : Thu, Mar 19, 1998 at 10:03 am
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 33 (complement) show homology to 2d11 bases 302 to 334 (33 in longest run, maxScore = 45). No substantial microsatellite (small CA and CT runs).
PCR Comments :



Length of sequence : 191  Consensus sequence is :

..

CATCACCTCT CTATGATGAA GCCATGCACA CTTCAATTAC ATTCGCCACA 
GAGGACCCCG CGACACACAC ACACGCACGC ACGCACCCCC CACACACACT 
AAGACTCCAT CTCTCTCTCT CACACACACA AACACAAACA CACACACACG 
CACGCACGCA CAGCCCCCCC CCACACACAC TAAGGCTCCA T

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wmc(19h1) Plate reference : 19h1
WMC locus number : WMC Length of sequence : 225
Last updated : Tue, Mar 3, 1998 at 12:09 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 225  Consensus sequence is :

..

GACTTTTCAA TAAATCTATA TGCTATGGAC CGAGGAGAGT GCCTAAGTCG 
AGAGAGATCA AGTGCGTGTG AAGGAGAATG AATAAGTGTG CAAAAAGAGT 
ATGTGTGTGA AAGAGAAAGT GACAAAAAAC AATAAATTAG AGAGAGTGTG 
TTTTTTCGTT TCTTATGTGA ACATATCACG CATGCATCTC CAAGATAGAA 
AAGCGAGGCG ACGAGATACA CGAAG

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wmc349(19a2) Plate reference : 19a2
WMC locus number : WMC349 Length of sequence : 187
Last updated : Mar 17 Nov 1998 at 11:01
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)22 123 to 166.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.
Forward primer details :
AcAcAcAcTcgATcgcAc at 86 length 18 bases, Tm 59
Reverse primer details :
gcAgTTgATcATcAAAAcAcA at 162 length 21 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 94 bases.



Length of sequence : 187  Consensus sequence is :

..

GTCGAGTGCT AAATCGGGGA GCCGAGCCGA GAGGGGTTTA ATTCGTGTGC 
GGCAAATTTC GTGATTCTTC CGTACCCACG TCCGTACACA CACTCGATCG 
CACCGGGCCT ATGGCCTCTT TTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTTTTG ATGATCAACT GCTCGTG

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wmc350(19b2) Plate reference : 19b2
WMC locus number : WMC350 Length of sequence : 328
Last updated : Lun 9 oct 2000 at 14:43
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 258 to 307 show homology to 1C2 bases 305 to 354 (50 in longest run, maxScore = 33), zone is 250-327. Reverse primer has a mismatch to 1C2. (CT)15 258 to 287. Bases 258 to 308 show homology to TW5d07 bases 159 to 209 (51 in longest run, maxScore = 22).
PCR Comments : 61C anneal, product.
Forward primer details :
gTcAgTccATcgAAgccTcc at 180 length 20 bases, Tm 64
Reverse primer details :
TccgAgAAAgAgcgAggTcA at 302 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 142 bases.



Length of sequence : 328  Consensus sequence is :

..

CACTCCCTCG AGACTGTCTC ACTCTTTCTT CCGCACGGCG GGCCACACTC 
GCTCAATCTC GCTCTCTTTA TCTGAGTCTC GTTTAACCTC GTTTTCTTTC 
ACACACAAAT GATATATCTC CCTGTTCGGG CCTCGATCAA CACACTCTAT 
ACTCTCTCTC ACGCAGATTG TCTATCTAGG TCAGTCCATC GAAGCCTCCC 
CCACTCTTTC GACCTCATGG CGGGCCACGC TCTCTCTCTC TCCCCCTCCC 
TCTCTCCCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTTTG TATGTCTCGA 
TTGACCTCGC TCTTTCTCGG ACAAAAAC

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wmc351(19c2) Plate reference : 19c2
WMC locus number : WMC351 Length of sequence : 319
Last updated : Mar 17 Nov 1998 at 11:01
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GGC)6 125 to 142.
PCR Comments : 61C anneal, product.
Forward primer details :
TgATcccATgcTcgAccT at 51 length 18 bases, Tm 62
Reverse primer details :
TggAgTcAccgggAAgTT at 225 length 18 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 192 bases.



Length of sequence : 319  Consensus sequence is :

..

GTCGTCGCCG TCGAGCACCC AGCGCAGATC GACGGACAGC GCCCTCCCCC 
TGATCCCATG CTCGACCTCG TTTCGGCAAA AAAGATAAGT AGGTTCCAGA 
GAGGAGCTAG GTCTGTGAGT GGGTGGCGGC GGCGGCGGCG GCACCAGCGC 
GGTTGCGTTG TGGGTTCCGG TGGCTTATAC AGCGAGCGGG ATAGGTTGAC 
GGCGCAGGGG CAAAAATGGC GACGAACTTC CCGGTGACTC CAGTACGCAG 
GTGATGTTTG TTCTCCTAGC AGTAAAAAAA TTGATTTTTT GGTTGGATCC 
GGGCGACCGC GGCCTGAAT

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wmc(19d2) Plate reference : 19d2
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Mar 3, 1998 at 12:10 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : No sequence 30.1.98
PCR Comments :
No sequence available for this clone
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wmc(19e2) Plate reference : 19e2
WMC locus number : WMC Length of sequence : 447
Last updated : Tue, Mar 3, 1998 at 12:10 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 447  Consensus sequence is :

..

GAAATAACCA GCGAAAAAAA ACCCGCACGT ACGAGCGACG TCACATGCCC 
TCGAGCGAGA ACGACTCGCA CGCTAGACTC CCTGCCCTCG TTCGTCGTCG 
CCTTCATCCC TTCGCCGCCG CCGCTCATCC CTTCGCCGCC GCCCTCGGTC 
TCACATCAAA GGCGCCCCAC TAGCACAACG AACCTGCCGC CCTTCTGGAG 
CCGAAAGCCG ATCCATATAT CCCCATCTGG ACGCGCACGT CCGCCTTCTT 
GGATCCATCG CGTGGATCCG CCGCCGCCGT CGCCGCGGGA CCCCTCGGAT 
GCCTCCACCT TCGTGGATCC ATTGCTGTCG TCGCGGATCC ACCTCTTCCT 
CCTTCGTTGC TGCCCCTCCG CCCCACCTGC CGGCATCGAC CTCATCCTGC 
ACGCCTACCT GGTCGGGGCC CCTGACTCGT CGTCCGCCAC CTGGTCG

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wmc(19f2) Plate reference : 19f2
WMC locus number : WMC Length of sequence : 263
Last updated : Tue, Mar 3, 1998 at 12:10 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 263  Consensus sequence is :

..

GGGTTTGAGG ACGGGGGAGA GAGCGGCCGT GGGAGAAGGA ACAGGTCGGG 
GAAGATTGAC GGGTTGCACC GCCGCCGCCG CCGCCACCAC CAAGACTCTG 
ACCACCGTTG CTGGTCGGAG TTCAACACTG CCCCAAGGGA GCCATCGACG 
GCGACATGGG GGAGAAGAAG AAAGGGATTT CGATCTCTTT CTGATCCAGA 
ACGCAACGGG GAATGGCAGC GTGTAACAGT CCAACCTGAC GAGTAGGCCC 
CACCGCGGTC AAA

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wmc(19g2) Plate reference : 19g2
WMC locus number : WMC Length of sequence : 200
Last updated : Mon, Mar 16, 1998 at 3:39 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 70 to 97 (complement) show homology to 17f9 bases 23 to 50 (28 in longest run, maxScore = 45). (CA)13 1 to 26. No room for primers.
PCR Comments :



Length of sequence : 200  Consensus sequence is :

..

CACACACACA CACACACACA CACACAGAGA GAGAGAGAGA ACCGGAGGGG 
TGGTGGAGTT GCTTCTCGGA GATGGTGGGA GAGGCCTACT AGACAAATTG 
AGGGTAAAAC TGCAATATCA AGAAGAGAGG GATGTGTTTG TTGAGAAATG 
TAGCATGCAA TTTCAAAAAA AATCCTACGC TCACGCAAGA TCTATCTAGG

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wmc352(19h2) Plate reference : 19h2
WMC locus number : WMC352 Length of sequence : 230
Last updated : Lun 30 oct 2000 at 16:59
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 150 to 204 show homology to 9f3.1 bases 222 to 276 (55 in longest run, maxScore = 60), zone is 135-230. Reverse primer has 2 mismatches to 9f3.1, forward primer is not with the region of homology. (GA)33 80 to 145. Bases 81 to 145 show homology to TW3g11 bases 164 to 228 (65 in longest run, maxScore = 32). Bases 10 to 145 show homology to TW1f09 bases 46 to 181 (136 in longest run, maxScore = 58). Bases 80 to 137 show homology to TW1f10 bases 147 to 204 (58 in longest run, maxScore = 52). Close enough to TW3g11 to classify as duplicate. 19H2 has priority Bases 108 to 138 show homology to TW2h08 bases 54 to 84 (31 in longest run, maxScore = 54). Bases 14 to 78 show homology to TW6b03 bases 48 to 112 (65 in longest run, maxScore = 57).
PCR Comments :
Forward primer details :
gTTTTTgTgTcggATgcgTg at 37 length 20 bases, Tm 64
Reverse primer details :
cTcccggAcTcATgTTcTcT at 141 length 20 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 124 bases.



Length of sequence : 230  Consensus sequence is :

..

GAGAGAGATG GAGAGAAAGG GAGAGGGACC AACAAGGTTT TTGTGTCGGA 
TGCGTGCGAC AGAATTGAAG ATTGTCGGCG AGAGAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAACATG 
AGTCCGGGAG AGGGGGAGGT CGCGTTTTAT GCATCAAAGA CTGATATAAA 
CCATTTTTCG ATATAAACTT GCATTCAAAT

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wmc(19a3) Plate reference : 19a3
WMC locus number : WMC Length of sequence : 117
Last updated : Mon, Mar 16, 1998 at 3:40 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 117 show homology to 19b3 bases 1 to 117 (complement) (117 in longest run, maxScore = 58). (CA)28 3 to 58. No room for primers.
PCR Comments :



Length of sequence : 117  Consensus sequence is :

..

CGCACACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CACACACATT CCAGACTGAT CTTGCTGTAT TTAATTTTTA CAAAAGCTAG 
AAAATTTGCT AGTTAGC

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wmc(19b3) Plate reference : 19b3
WMC locus number : WMC Length of sequence : 117
Last updated : Mon, Mar 16, 1998 at 3:41 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 117 (complement) show homology to 19a3 bases 1 to 117 (117 in longest run, maxScore = 58). (GT)27 61 to 114. No room for primers.
PCR Comments :



Length of sequence : 117  Consensus sequence is :

..

GCTAACTAGC AAATTTTCTA GCTTTTGTAA AAATTAAATA CAGCAAGATC 
AGTCTGGAAT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGCG

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wmc353(19c3) Plate reference : 19c3
WMC locus number : WMC353 Length of sequence : 207
Last updated : Mar 17 Nov 1998 at 11:01
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (note Rsa1 sites at 23-27, and 27-30). Bases 27 to 64 (complement) show homology to 4d10 bases 145 to 182 (38 in longest run, maxScore = 53), zone is 27-134. Forward primer has 2 mismatches to 4d10, reverse primer is outside this region. (GT)18 106 to 141. Bases 7 to 49 show homology to 22d9 bases 10 to 52 (43 in longest run, maxScore = 54).
PCR Comments : 61C anneal, product.
Forward primer details :
gcTTcgcAcTcgcATgATTT at 55 length 20 bases, Tm 65
Reverse primer details :
AgTAggTcgATcTgTcgcTcTc at 164 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 131 bases.



Length of sequence : 207  Consensus sequence is :

..

ATTCACCACC TCTTCTCTTC TTGTACGTAC TCCATTTGTA TCTCACTTCC 
TGTGGCTTCG CACTCGCATG ATTTTCCTAT TCTATGAACA TGTGTGTGCA 
TGTGCGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TTTAACAACA 
TGTGTGTGTT AGAGAGAGCG ACAGATCGAC CTACTCCTAC AGAGAGATGG 
TGTGTAG

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wmc354(19d3) Plate reference : 19d3
WMC locus number : WMC354 Length of sequence : 188
Last updated : Mar 17 Nov 1998 at 11:01
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimed to insert at both ends. Bases 53 to 80 show homology to 3b11 bases 95 to 122 (28 in longest run, maxScore = 41), zone is 3 - 93. Forward primer has 4 mismatches to 3b11, reverse primer is outside of the zone of homology. (GT)12 93 to 116. Bases 128 to 155 show homology to 24g3 bases 34 to 61 (complement) (28 in longest run, maxScore = 46).
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
cAATggAgTccTTgTggTgg at 26 length 20 bases, Tm 63
Reverse primer details :
AgcATTTcAcTcccgcgTAg at 166 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 160 bases.



Length of sequence : 188  Consensus sequence is :

..

GGACGGGCAA ACTGGTAGAT GTTGACAATG GAGTCCTTGT GGTGGCGGCG 
GCAGCGGCGG CGGGGACTTT GCTAGGGTTT CGAGGGAGGG AGGTGTGTGT 
GTGTGTGTGT GTGTGTGGAG GGGGGTTGGG TGTTTGAGGA AATGACGCGG 
GTACTCAAAA TTTTACTACG CGGGAGTGAA ATGCTGGG

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wmc(19e3) Plate reference : 19e3
WMC locus number : WMC Length of sequence : 472
Last updated : Tue, Mar 3, 1998 at 12:10 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 472  Consensus sequence is :

..

TAGAATCCAA CACCGAATAG AGCCGTGCCA CCGGAACTCC TTGAGCAACA 
AGATGCCGCC TCCAAGAAGA GGACGACGCT ATGACGCCGC CGTCGCCGCC 
GCCTGGTCCA AAGGACGGAC CAAGGGTTTC CCCTTAGCAC GGCAAGGCTA 
CAATGAATGA TGACACATAC CACACAAATA TGATCCACCA CTTATTCAAT 
AATTCAAATG CCTTAGCAAA TGTTATGCAT AGACCAAAAA AATGCACAAT 
GACAATCCAA TGCTGACTTT GATGGCATTC AATTTTGCAA CTTATACCTA 
AAGTTTCAAT AATGCCCAAC TATCTATATT GAAGTAAGTT TTTTCTTCTT 
TTTTGTGATG TCCAGATTTT TTCATATATT TGTATTGTTC TTCCAATAAA 
ATGCACGGGC ACGGCAACGC GCGCCCTTAT GTTCTAGTTA AATCAGGAAA 
CTAGGTGGAC ATGCTGGGTT GC

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