Wheat microsatellite WMC 25/06/01

wmc(19f3) Plate reference : 19f3
WMC locus number : WMC Length of sequence : 37
Last updated : Tue, Mar 3, 1998 at 12:11 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. NB 5' linker corrupted. No substantial microsatellite.
PCR Comments :



Length of sequence : 37  Consensus sequence is :

..

GGAGTAGCCA CGAGAGGAGC ATGCGTTACC CTGGCGC

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wmc(19g3) Plate reference : 19g3
WMC locus number : WMC Length of sequence : 394
Last updated : Thu, Mar 19, 1998 at 11:41 am
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 117 to 164 show homology to 3e12 bases 158 to 205 (48 in longest run, maxScore = 68), zone is 77-214. (CA)26 65 to 116, (CA)9 228 to 245, (CA)8 375 to 390. Sequence is full of multplie internal repeats, too difficult to design primers.
PCR Comments :



Length of sequence : 394  Consensus sequence is :

..

CACACACCTC TCCCCTAGTT ACGAGTCGAT GGTCAACTTG CAACTAGGGG 
ACACGACGAA TAAACACACA CACACACACA CACACACACA CACACACACA 
CACACACACA CACACACCTA GTTGCGAGTC GATGGGCAAC TTGCAACTGG 
GGGTCCTCAA CAAAACACAC CCCCCTAGTT GTGAGTCGGT GCTTGACATG 
CAACTGGGGA CCCCCTTGAC AGAAAGACAC ACACACACAC ACACACCTCT 
AGTTGCAAGT CGGTTATCCG ACATGCAATT GGGGACCCTC AACACACACA 
CACACACGCA CGCACATCTC CCCCCTAGTT GCGAGTCGAT GGTCAACTTG 
CAACTAGGGG CCACGAATAA AATACACACA CACACACACA TCCC

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wmc(19h3) Plate reference : 19h3
WMC locus number : WMC Length of sequence : 142
Last updated : Mon, Mar 16, 1998 at 3:44 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 14 to 142 (complement) show homology to 13g5 bases 117 to 245 (129 in longest run, maxScore = 57). (CA)30 13 to 72. No room for primers.
PCR Comments :



Length of sequence : 142  Consensus sequence is :

..

CACACACACA TACACACACA CACACACACA CACACACACA CACACACACA 
CACACACACA CACACACACA CAAAGAGTTT AATAGTTCAC TCTCACGTGC 
TCCGCTACCT GCCTCTCCAC CTGTTCCCTC TGCACCTCTA AT

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wmc(19a4) Plate reference : 19a4
WMC locus number : WMC Length of sequence : 205
Last updated : Mon, Mar 16, 1998 at 3:45 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 37 to 94 show homology to 13f4 bases 93 to 150 (58 in longest run, maxScore = 100). (CA)10 3 to 22. No room for primers.
PCR Comments :



Length of sequence : 205  Consensus sequence is :

..

CCCACACACA CACACACACA CATTGAGACT CCACCTCACA CACACATGCT 
CTAGGCGGAG CATTTGTTCC TACCACCCTT CATCCACCCT TACCTGTACG 
ATAAACAATC ACCTTAATTT ACTTGTATAA CATGGACAAA TTCCAATTTA 
CTTTAGTAGT TACCAAACTT ATCATATGTA CACTTATAAA AAACCGAGTT 
GGCGA

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wmc355(19b4) Plate reference : 19b4
WMC locus number : WMC355 Length of sequence : 381
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 280 to 305 show homology to 14g5.1 bases 195 to 220 (26 in longest run, maxScore = 68), zone is 118-335. Forward primer has 5 mismatches to 14g5.1, reverse primer is outside this zone of homology. (CA)14 280 to 307. Bases 216 to 228 show homology to 2b1 bases 153 to 165 (complement) (13 in longest run, maxScore = 25).
PCR Comments : 51C anneal, product.
Forward primer details :
gATAgTcgAAcgTTgTAggTAggT at 132 length 24 bases, Tm 59
Reverse primer details :
gAAgAgAgTggAggTATcATTggT at 343 length 24 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 235 bases.



Length of sequence : 381  Consensus sequence is :

..

CACATATGCA TGGATGTCGA TCGATCTCCC TCAATACATA AAATAGGTAT 
CTCACCTCCT CCACATATAT ACCAGCCATT CTACCTCTAT AGTTAGGTCT 
CTGCCCCCCC ACCCCTACCC CCACACACAC CGATAGTCGA ACGTTGTAGG 
TAGGTATCCA TCCCACAGAG ACAAACTACA CTTGTCCCCT CTCACGTTCC 
AGTAGGCCAG CCACCCTATA TCTTTGACGT GGGCACAAAC AAATTCGCTG 
GCGCTCTTTA TCGATCGCGT GCGCGCGCGC ACACACACAC ACACACACAC 
ACACACACTT CATCCCTCTC TTCGATTCTA TGGATACCTC ACACCAATGA 
TACCTCCACT CTCTTCTCAT GGATCGCCCC C

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wmc356(19c4) Plate reference : 19c4
WMC locus number : WMC356 Length of sequence : 415
Last updated : Mar 17 Nov 1998 at 11:01
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)23 146 to 191, (GT)8 206 to 221.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (there appear to be nulls of this locus in many wheats).
Forward primer details :
gccgTTgcccAATgTAgAAg at 67 length 20 bases, Tm 65
Reverse primer details :
ccAgAgAAAcTcgccgTgTc at 289 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 242 bases.



Length of sequence : 415  Consensus sequence is :

..

CGACATAGAG CGGCTATTGC AGATAGCCTA CTTCCTGCTG GATGTTCCCA 
AGGATCTCTT TCAGGGGCCG TTGCCCAATG TAGAAGTATA TCGTGTTGGC 
TGGCACCGAA CCTTTTTCTC GCTCGCCTCG TGCGTGTGTG TGTATGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGCGTGTGCG 
TGTGCGTGTG TGTGTGTGTG TGGACACCTC TCCCTCGCCC TAGCACAAAC 
CGAGAGGAAG GAGGAGGAAG AACAGCGAGT GCACGATGGA CACGGCGAGT 
TTCTCTGGCG CACGAACGCC CGCCGCAACG ATGGACTATT TCCTCAAGTA 
ACAACTCGAG CCACACCGCC AACATACAGG GGAGGGAGGG GTCTTTTATG 
CGGGCGCGCA CTGAC

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wmc(19d4) Plate reference : 19d4
WMC locus number : WMC Length of sequence : 169
Last updated : Thu, Mar 19, 1998 at 1:44 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 93 to 106 (complement) show homology to 5c12 bases 284 to 297 (14 in longest run, maxScore = 19). (TTG)10 2 to 31, (TTG)11 68 to 100, (TTG)8 146 to 169. No room for primers.
PCR Comments :



Length of sequence : 169  Consensus sequence is :

..

GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GCTGCTGTCG TGGTTGCTGT 
TGTTGTTGTT GTTGCTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG 
CTGTTGTTGT TCCTGTTGTT TCTGTTGCTG TTGTTGTTGT TGCTGTTGTT 
GTTGTTGTTG TTGTTGTTG

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wmc(19e4) Plate reference : 19e4
WMC locus number : WMC Length of sequence : 179
Last updated : Mon, Mar 16, 1998 at 3:49 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 74 to 106 show homology to 5b8 bases 306 to 338 (33 in longest run, maxScore = 53), zone is 54-179. (GT)13 44 to 69. Too difficult to design primers (internal repeat structure).
PCR Comments :



Length of sequence : 179  Consensus sequence is :

..

ATTGATGTGC CACCCTACTC CTTCGGTGTC GGGTAGTGTG TGCGTGTGTG 
TGTGTGTGTG TGTGTGTGTT TGGGAGAGAA GCCAGTCGAT AGATTAGTAT 
CAAGATTAAG GTGTCACCTT ACTCCTTCGG TGTTGGGAAG TGTGTGTGTG 
TGGGTGTGTG GGGGGGGCAG TGACACTCA

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wmc357(19f4) Plate reference : 19f4
WMC locus number : WMC357 Length of sequence : 365
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GA)10 302 to 321, (GT)10 266 to 285.
PCR Comments : 61C anneal, product.
Forward primer details :
TAgTgggTgAccggTcAAgA at 159 length 20 bases, Tm 63
Reverse primer details :
TggAcggATTTggTcATTTc at 343 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 204 bases.



Length of sequence : 365  Consensus sequence is :

..

CTCCCCGGCT CGCAACATAG CGGGTGGCCA ATCTATAGGA GGGGAGGTTG 
CAGCACCATG GCTAGAGCTT GGGCCATGGA AGATTTGGGA AGAAGACGGT 
TTTTGCAGCG GCAGGTGGCT ACGCATTTTG CTCATGGTCG TCTCCCTCGA 
CTCGCAAATA GTGGGTGACC GGTCAAGAGG AGCGGAAGTT TGTAGCGCCA 
TGGTTGGAGC TTGGGCCGTG GAGGATTTGG GAAGTAGATT GGAGGGGCAT 
GCGTGCCTAT GTGTCGTGTG TGTGTGTGTG TGTGTGAGCG AGAGAGAGAT 
AGAGAGAGAG AGAGAGAGAG AGCGAGGGGG TCAGACGAGG GAGAAATGAC 
CAAATCCGTC CAAAT

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wmc358(19g4) Plate reference : 19g4
WMC locus number : WMC358 Length of sequence : 256
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 61 to 113 (complement) show homology to 11d10 bases 103 to 155 (53 in longest run, maxScore = 79), zone is 44-214. Primers are outside of this region. (GT)9 175 to 192, plus (GA)3G(GA)7.
PCR Comments : 51C anneal, product.
Forward primer details :
TgcTAgTAgggAgAAggAgTTT at 7 length 22 bases, Tm 58
Reverse primer details :
ccTTTTgTTTTgAgATTTcgcT at 214 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 229 bases.



Length of sequence : 256  Consensus sequence is :

..

GCATCTTGCT AGTAGGGAGA AGGAGTTTGT GTGACACATA TCTAGCTATA 
TTAAGGGATA GGTAGATAGC ATTTGTGCGA GGCATACTTA AAGCATCACG 
AAGATAAACA AAATTGTGTG CATGCAAGTG CCGGAAAAAA CGAAGCGAGA 
AACAATGTGT GTGTGCGCGC GTGCGTGTGT GTGTGTGTGT GTGAGAGAGG 
GAGAGAGAGA GAGAGCGAAA TCTCAAAACA AAAGGTGCTT TTTTGGAATC 
CCATTG

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wmc359(19h4) Plate reference : 19h4
WMC locus number : WMC359 Length of sequence : 134
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). Bases 78 to 100 (complement) show homology to 1C2 bases 783 to 805 (23 in longest run, maxScore = 20), zone is 77-134. Reverse primer has 2 mismatches to 1c2, reverse primer is outside of this homology. (CA)25 38 to 87.
PCR Comments : 61C anneal, product.
Forward primer details :
TTgcAcATTcAcAccTcTccAT at 10 length 22 bases, Tm
Reverse primer details :
TgTTgTggcAgAAgggcA at 100 length 18 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 108 bases.



Length of sequence : 134  Consensus sequence is :

..

CATGATCATT TGCACATTCA CACCTCTCCA TGACTATCAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACCG TCTCCACATT 
GCCCTTCTGC CACAACACAC ATGTAGACAC ATAC

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wmc(19a5) Plate reference : 19a5
WMC locus number : WMC Length of sequence : 126
Last updated : Lun 2 Nov 1998 at 10:03
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 76 to 123 show homology to 11b5 bases 140 to 187 (48 in longest run, maxScore = 21). (TTG)8 16 to 39, (TTG)9 43 to 69, (TTG)10 73 to 102. No room for primers. Bases 101 to 126 show homology to 21h6 bases 47 to 72 (26 in longest run, maxScore = 23).
PCR Comments :



Length of sequence : 126  Consensus sequence is :

..

GTGGTGTTGT TGCTGTTGTT GTTGTTGTTG TTGTTGTTGG TGTTGTTGTT 
GTTGTTGTTG TTGTTGTTGC TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT 
TGGTGGTGGT GGTGGTGGTG GTGGTG

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wmc(19b5) Plate reference : 19b5
WMC locus number : WMC Length of sequence : 103
Last updated : Lun 30 oct 2000 at 16:59
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. NB 5' linker corrupted. (GT)14 74 to 101. No room for primers. Bases 28 to 42 show homology to TW7h10.2 bases 42 to 56 (15 in longest run, maxScore = 30).
PCR Comments :



Length of sequence : 103  Consensus sequence is :

..

TTGCCTCCCG ATGAACATGA TGCATTCCGT TGCCTCCCCA TGAACACGAC 
GCATTCCGAC CCAGCCGGTC TCTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGG

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wmc(19c5) Plate reference : 19c5
WMC locus number : WMC Length of sequence : 452
Last updated : Thu, Mar 19, 1998 at 3:10 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (NB 3' linker corrupted) Bases 361 to 378 (complement) show homology to 3c4 bases 74 to 91 (18 in longest run, maxScore = 27), zone is 341-381. (CA)12 416 to 439, (CT)10 223 to 242, (CT)10 396 to 415. Too difficult to design primers.
PCR Comments :



Length of sequence : 452  Consensus sequence is :

..

CAACCCCACC ATCGACTGGA TGGGCACACC CATCTACAAC AGAGACCAAA 
GGAAAAGCAA GAGCATGATT ACTAAGTTGG AAAGAAAGGA AGAACAAAAC 
ACAAATCAAG AAAATAAAGT AGGAGATCAT CACCACATAT AGGACGCATC 
TGACGACGAA TTCCCTGCCA CCGCACCTCC TTTCCTCTCC CTCCTCTCCT 
CCTCCTCTCT CTCTCTTATC TTCTCTCTCT CTCTCTCTCT CTCCTGCAGA 
TCAAATCGAG AGAGAAAGGA GAGGAGAGAG GAGGCGGGCG GCGGCAGGAG 
AAGAGGCGGC GGCGGGGGTC ACGGACTAGG TCTTTTTCTC TTGAATACAT 
ACAATGGGAA TCCAGCAGAG CACCTTTTTT TTGGGATTTC GCGTTCTCTC 
TCTCTCTCTC TCTCTCACAC ACACACACAC ACACACACAC CCGCGCACGC 
GC

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wmc(19d5) Plate reference : 19d5
WMC locus number : WMC Length of sequence : 136
Last updated : Mon, Mar 16, 1998 at 3:56 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. NB 3' linker corrupted. Bases 1 to 91 show homology to 7f1 bases 83 to 173 (91 in longest run, maxScore = 75). (GT)22 81 to 124. No room for primers.
PCR Comments :



Length of sequence : 136  Consensus sequence is :

..

ATTTGCAAAG GATATCCACT AGTGTAGCTT ACAAAGGTGC GTTCAAAATT 
ACAGAAGACT AAATCATGTA TTGAATTGAA GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTTTAAAA CTTGAA

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wmc360(19e5) Plate reference : 19e5
WMC locus number : WMC360 Length of sequence : 153
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)22 54 to 97. Software struggled to get these primers.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.
Forward primer details :
TATgTgTgTgAAAAATgg at 2 length 18 bases, Tm 50
Reverse primer details :
AATTTcTTgTAgAAccgA at 136 length 18 bases, Tm 50
Expected size of amplified fragment (Chinese Spring=) 152 bases.



Length of sequence : 153  Consensus sequence is :

..

GTATGTGTGT GAAAAATGGT CGTTGTGAGA CATTCAATGA TATATATATA 
TATGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTTTG 
TGTGTGTGTG TGTATGTACA TATATACATA TATGATCGGT TCTACAAGAA 
ATT

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wmc(19f5) Plate reference : 19f5
WMC locus number : WMC Length of sequence : 160
Last updated : Tue, Mar 3, 1998 at 12:12 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 83 to 114 (complement) show homology to 7h1 bases 100 to 131 (32 in longest run, maxScore = 46). No substantial microsatellite.
PCR Comments :



Length of sequence : 160  Consensus sequence is :

..

CCTCGATCTT GATACTAATC TATCGACTGG ATTCTCTCTC AAACGTACAC 
ACACACACAC ACTACCCGGC ACTGAAGGAC TTGGGTGGCA CATCGATCTT 
GATAATAATC TATCGACTCC TCTTTCAAAC ACGCACACAC ACACACTCGC 
TAGTTGTGCC

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wmc(19g5) Plate reference : 19g5
WMC locus number : WMC Length of sequence : 353
Last updated : Tue, Mar 3, 1998 at 12:12 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 38 to 61 show homology to 7e5 bases 53 to 76 (24 in longest run, maxScore = 21). No substantial microsatellite.
PCR Comments :



Length of sequence : 353  Consensus sequence is :

..

ACCGGAGCTG GGGTGGCCTG TAGCAGCGGC GGCGGCGAGT TGGGCGGCGG 
CCGGAGTTCG GCCGGCAATG GTTTGGGCGA TGCCAAGCAA GGGAGGAGCC 
GTGGTTAGTC GTGTTCGACT CACGGAAGTG CGGTGAGTGC AACGGTGAGC 
TCGAATGCCA AGTTGCGCGG CCGTAGCTAC GATGGCGACA TGGCCGGCGG 
CGGGGAGCTT CAGGCTTGAT CGGAAGATGG GGCTAGGGAA CAGGAACGGA 
GGGGGAGAGT GAGGCGGAAA CAACAGGAGC TCACAAGGAG GTCGAAGAAC 
GGCTCAGCGG GCTTGGGGAC GTCCTCGATG GCGCGAATCG ACCGGCGGTG 
ACC

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wmc(19h5) Plate reference : 19h5
WMC locus number : WMC Length of sequence : 168
Last updated : Mon, Mar 16, 1998 at 3:59 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 58 to 102 (complement) show homology to 7b1 bases 53 to 97 (45 in longest run, maxScore = 47). (GT)8 109 to 124, plus small (GT) runs. No room for primers.
PCR Comments :



Length of sequence : 168  Consensus sequence is :

..

ATTTAACATC TTTTCCATAT AATTCATCTA ACATTAACAT TCTTATCTAC 
GTAATTTATC TAACATTAAT CTAAACAACA GAAAACAGAA AATAAGTAAA 
AACAATATGT GTGTGTGTGT GTGTCTTTGT GTGTGTGTAT GTGTGTGTGT 
ATGTGTGTGT GCACGAGC

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wmc(19a6) Plate reference : 19a6
WMC locus number : WMC Length of sequence : 186
Last updated : Tue, Mar 3, 1998 at 12:12 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 133 to 164 show homology to 1E3 bases 1 to 32 (32 in longest run, maxScore = 27). Bases 133 to 185 show homology to 12e12 bases 1 to 53 (53 in longest run, maxScore = 44). No substantial microsatellite.
PCR Comments :



Length of sequence : 186  Consensus sequence is :

..

CACCTCCGGT GTAGTAGGAG TCGGCGGCGG CGGTGGCGTC TGGCTCCTGG 
GCTCCAACAT CTGGTTGCGG CAACCAGGTA GAAGGGAAAG GGGGAAAAGA 
GGGAGAAAAG CAACCGTGAG TACTCATCCA AAGTACTCGC AAGCAAGGAG 
CTACACTACA TATGCATGGG TATATGTGTA AAGGGC

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wmc(19b6) Plate reference : 19b6
WMC locus number : WMC Length of sequence : 226
Last updated : Tue, Mar 3, 1998 at 12:12 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 226  Consensus sequence is :

..

CAGAGAGAGG GAGGGGGAGA AGAGAGGTGG AGGGAGAACC TACCAGGATT 
TGAAAGCTCG ACGACGGCGC TGTGGTCACC GGAGGCAGCA CACCATCGAA 
CAGAAAGCTT CGCGTGTAGA GGGGACGGGG GAAGAGAACT CGGGGAGAAG 
CTATTCACCG GCGGGGGGTG CATCACCGGC GGCCGAGAAG GTGGGGCTCA 
TGGCGGCGGC GACGGCGGCG GGCGTC

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wmc(19c6) Plate reference : 19c6
WMC locus number : WMC Length of sequence : 360
Last updated : Tue, Mar 3, 1998 at 12:13 pm
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 360  Consensus sequence is :

..

ATTTAATGGT TGTCTTGAGT ATTGCTTGTG ATTGTGCTGT TTGCTTCCTC 
AACATGTCAA CTTCATCTCT AAGTGCAGAA GCAGAATCTC TTTCTACCAC 
TAGTTTAGCC TCTAGAGCAT CAGCAGTTGG CAATTCATGA TGCACGATTG 
GGCCGGTGCC ACTGCTGTGG GATGATACAA CGTCCATGGG GACATCGCTC 
TTTGGTCTTG GGCAAACATG TTTTGCCATT AAAGTTCCGA TTGGATTCCG 
AAAAGTTGAA GTTAGCAAAA CATCTCAACT GGGAGTTATA ACAGCAAACC 
AGTTAAACAA ACAAGGAATT AAAGAGTTTC AATTGGATGC TGAAAAGTAG 
TTAGTCCACG

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wmc(19d6) Plate reference : 19d6
WMC locus number : WMC Length of sequence : 760
Last updated : Mar 30 mai 2000 at 9:54
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Trimmed to insert at both ends. Note linkers corrupted at both ends. Bases 514 to 760 show homology to 17d8.2 bases 264 to 510 (247 in longest run, maxScore = 204). No substantial microsatellite. Bases 478 to 760 show homology to 22a3.2 bases 535 to 817 (283 in longest run, maxScore = 239). Bases 6 to 42 show homology to TW4f10.2 bases 241 to 277 (37 in longest run, maxScore = 39).
PCR Comments :



Length of sequence : 760  Consensus sequence is :

..

GAGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTG GCTGTAATCA 
TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 
CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 
GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCAACG CGCGGGGAGA 
GGCGGTTTGC GTATTGGGCG CTCTTCGCTT CTCGCTCACT GACTCGCTGC 
GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 
ATACGGTTAT CACAGAATCA GGGGATAAC
(break)
GTAAAACCTC TACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA 
AGCGGATGCC GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGC 
GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 
AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT 
ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG 
CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG 
TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC 
GTTGTAAAAC GACGGCCAGT GAATTCCTCG A

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wmc361(19e6) Plate reference : 19e6
WMC locus number : WMC361 Length of sequence : 482
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)10 327 to 346.
PCR Comments : 61C anneal, product; 61C anneal, polymorphic on acrylamide.
Forward primer details :
AATgAAgATgcAAATcgAcggc at 214 length 22 bases, Tm 67
Reverse primer details :
ATTcTcgcAcTgAAAAcAgggg at 408 length 22 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 216 bases.



Length of sequence : 482  Consensus sequence is :

..

CAAACTCATA CGCAAAAAGA TAGGTCTAAT AATTTCAGCA AAGAGTTCAA 
AATCCTCATC ATCCTTTTTC TTGGATGGTT TAGGAGGAAA AGGCATGGGC 
ACCGGGGCGG TGAAGGAGTG CGCGCTGGGC GTGCTCCGGA GCCATCCCCG 
GGTGGTGAGG ATCGAGGACG AGAGCCCCCT GAACTACGGC TGCACCGTCG 
CCTACATCGA GTGAATGAAG ATGCAAATCG ACGGCGGCGC CGCTCGAGTC 
CGTACCTACA GCAGCGACAG CTATACATTT TACAGATCGT AGACAACAGA 
GTTCTCCTGC GTGTTGTAGA GCAGAACACA CACACACACA CACACAGCAG 
AGTTGGAGCT ACATACATAG AGAAGAAAAA AAGGGAAAAT TACGCAGAGT 
TTTTGGGCCC CTGTTTTCAG TGCGAGAATT GAACTGTTAC ATTAGAAGAT 
ATCCACTGGG CGGAATTGAG CCGGCCTGGC GA

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wmc362(19f6) Plate reference : 19f6
WMC locus number : WMC362 Length of sequence : 225
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)44 90 to 177. Software struggled to get these primers.
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.
Forward primer details :
TcTATAATAATATgTATggTgTTcA at 21 length 25 bases, Tm 52
Reverse primer details :
TAgcTTcATgTTAcAAAcTAATcTc at 179 length 25 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 183 bases.



Length of sequence : 225  Consensus sequence is :

..

ATTGCTGGCT AAGTAATTAA TCTATAATAA TATGTATGGT GTTCATGAGA 
TGCATGAACT ATAGTATTTG TTATATGTAC ATTGTACATG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTTGA GATTAGTTTG TAACATGAAG 
CTACTTTAGT TCAGTTCCAT GTAAT

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wmc(19g6) Plate reference : 19g6
WMC locus number : WMC Length of sequence : 426
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 63 to 92 (complement) show homology to 8c12 bases 129 to 158 (30 in longest run, maxScore = 66), zone is 4-158. (CA)8 77 to 92, (CA)8 95 to 110, (CA)9 295 to 312, (CT)9 277 to 294. Sequence contains perfect internal duplication, too difficult to design primers. Bases 218 to 238 show homology to 20f9 bases 48 to 68 (complement) (21 in longest run, maxScore = 35).
PCR Comments :



Length of sequence : 426  Consensus sequence is :

..

CTAGCTACCC GACACCGAAA GAGTAGGGTG GCACCTTGAT CTTGATAGTA 
ATCTATCTAC TAGCTTCTCT CTCAAACACA CACACACACA CATACACACA 
CACACACACA CTCGCTAGTT GTGCCTCTGT GCCTACCTTG CGCACACTCT 
CGACACGTCT TGATCTTAAT CTATCAATTG GTTTCTCTCT CAGACACACA 
CACACTACCC GACACCGAAG GAGCAGGGTG GCACCTCGAT CTTGATAGTA 
ATCTATCTAT TGGCTTCTCT CTCAAACTCT CTCTCTCTCT CTCTCACACA 
CACACACACA CACCCACACT CGTTAGTTGT GCCTCTGTGC CTACCGTGCG 
CACACTCTCG ATACGTCTTT CTCTCCCTCC CCCTCCCCAC TCCCCAACAA 
TGATAGCATA AGGGTGACAA CTCAAT

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wmc363(19h6) Plate reference : 19h6
WMC locus number : WMC363 Length of sequence : 169
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Pioneer Date at Agrogene : 30.1.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)16 40 to 71.
PCR Comments :
Forward primer details :
TcTgTAAcgcATAATAgAATAgccc at 3 length 25 bases, Tm 61
Reverse primer details :
ATgATTgcgTTATcTTcATATTTgg at 86 length 25 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 108 bases.



Length of sequence : 169  Consensus sequence is :

..

ATTCTGTAAC GCATAATAGA ATAGCCCAGA TGTGTGTGCG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TAACTTGAAG ACAATCCAAA TATGAAGATA 
ACGCAATCAT GACGCCAAAT GAAAATCTTC AAGAAAGTGT GGTGGTGGCG 
TTACCCACCG TATAGAGAT

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wmc(12A7) Plate reference : 12A7
WMC locus number : WMC Length of sequence : 215
Last updated : Mar 25 Aož 1998 at 11:00
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 39 to 111 show homology to 9d1 bases 47 to 119 (73 in longest run, maxScore = 97). (GT)8 165 to 180, (GT)14 187 to 214. No room for primer.
PCR Comments :



Length of sequence : 215  Consensus sequence is :

..

GTACTACTTT AAGTGAATCC TTATTATAAC ATGCACACAA TTTTGGGCAC 
TTGTATTCGA CTTAAGTCAG TGTGCCACCG TATCTCGTAT ACTTACTACT 
CCCTCCGTCT ACGTGTAATA AGTCACGTTA GAAGGTGCAA CAGGACCAAG 
GTGCGTACGT GTGCGTGTGT GTGTGTGTGT ATGTATGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTG

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wmc(12b7) Plate reference : 12b7
WMC locus number : WMC Length of sequence : 385
Last updated : Mar 25 Aož 1998 at 11:05
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end and to insert at 5' end. Bases 1 to 385 (complement) show homology to 12c6 bases 1 to 385 (385 in longest run, maxScore = 291). (CA)21 238 to 279. True duplicate, 12c6 has precedence.
PCR Comments :



Length of sequence : 385  Consensus sequence is :

..

CATGATATAA TCAACGAAAG TGAAGGATAA CAATATCATG CATCTCTAGT 
TTAACTCACC TGGGTGGAAT GATCATGAAT ATGCATCAAG TTAAAGAATT 
ACTCTTGAAG AACAGGTTAC ATATATCAAT ATATCTGCCG AGGGCTGAAC 
CATCCGGGTT CACCCTATAT GCCAAGTCAC CAAACTTGGT CATATCATTG 
CTATCATAGC AACACCTTTC TCATCACCAC ACACACGCAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC TTGGTTTATG CGGAAATATC 
AGGACGTAGT TTAAGCAGGA TTACCCAACT GTCCTTGACC GTGGACACGG 
CTATTCGAAT AGTTTACACT CTGCAGAGGT AGTAC

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wmc(12c7) Plate reference : 12c7
WMC locus number : WMC Length of sequence : 270
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 152 to 266 (complement) show homology to 9h1 bases 1 to 115 (115 in longest run, maxScore = 88), true duplicate, 9h1 has precedence. (CA)22 42 to 85, (CA)8 150 to 165. Bases 55 to 85 show homology to 2b4 bases 232 to 262 (31 in longest run, maxScore = 24).
PCR Comments :



Length of sequence : 270  Consensus sequence is :

..

GTACCCGCGT CATTTCCTCA AACACACCCC ACACTCCCCT CCACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACCAAA ACCTCCTCAG 
GTGCGCGCGT GCACACACAC ACACACCAAA ACCACCTCAG GTGTGCGCGC 
ACACACACAC ACACACCCTC CCTCGCTCAA AATCCTAGCA AGGTCCCCGC 
CACCGCCGCC GCAAGGCCTC CATCGTCAAC ATCTGCCGTT TTGCCCGTGC 
AGCTTACCCA TCGATCTCCA

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wmc364(12d7) Plate reference : 12d7
WMC locus number : WMC364 Length of sequence : 598
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 32 to 41 (complement) show homology to 13e1 bases 81 to 90 (10 in longest run, maxScore = 29). (CA)18 93 to 128.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
ATcAcAATgcTggcccTAAAAc at 41 length 22 bases, Tm 64
Reverse primer details :
cAgTgccAAAATgTcgAAAgTc at 223 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 204 bases.



Length of sequence : 598  Consensus sequence is :

..

GTACAAGTGG ATGAGCAAGG CTCTATCTCC AAAATGAGCT ATCACAATGC 
TGGCCCTAAA ACGTTGGGGG AGAAGGAGTA TACACACACA CGCACACACA 
CACACACACA CACACACACA CACACACACG CACACACGCA CACTTTGCAC 
ACACATGCGT CGGACGTCCG ACCGTTACTG GTCGTCCGGT GGCTCGAGGG 
TCCGAACGTC CGGTAATGCT TGGACTTTCG ACATTTTGGC ACTGATTAGG 
TGGTGCCAAA TATCCAGAGG CAGTCGGACA TCCGGTGGGT CGGTCGTCCG 
GTGGACTTCA TATTTCTGTT GTTTTTGTTT TGTGATGGAT GTGTCGGATT 
TCCGGAGATG GCCGGTTGTC CGATGGCTGG AGCATGTCCA ATTTCTGGTC 
CTTGTCGATC GTCCGGACAA TGTTGCATAA ATGGCTTGGC CTTCTGGCTG 
GTGGGACTTC CAGGCATCAG ACGTCCAAGG AGTTTCAGTC CTCCAGTGGT 
TGTAGGTTCC TGGCAGCTCT TCCTCTTGGT CCTCCCACTC CTTGTTCTCG 
CCATCTTGTC CATTAAGTGT TGTTGCTCCG TGGCTTCCCT CCCAGTAC

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wmc(12e7) Plate reference : 12e7
WMC locus number : WMC Length of sequence : 352
Last updated : Tue, Mar 3, 1998 at 12:14 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (NB linker 3' corrupted). Bases 119 to 284 show homology to 9d5 bases 118 to 283 (166 in longest run, maxScore = 203). No substantial microsatellite.
PCR Comments :



Length of sequence : 352  Consensus sequence is :

..

GATAACCGCG CTGATCTCCT TAACCATTTT CTCTCGAGAG AGGGACGTGA 
GGAGGAGGGG GCGAAAAGAG AAGTCACTTC CGACCCCTAA GCTGACATGG 
CGGCGGCGGC GGCCGGAAAT TTCGAAAAGC GCGTACGCCC GGCGTCTCCG 
ATCTTATATT GGAGCGGTCG TGGCGGTGGT AGGAGGCCCC GATGCCACGG 
GTGGTTAGGA AGAAGACGGT TCAATCACGG GATTTTGGGA GAGGTGGCGA 
GCGTGAGGTA TGGGATCCTT TAACATATAT CATCCAGATA AAAAAAAGAA 
CATTATGTTG TGCTCAAAAA ATACATTTCA TAAGGGTGAA CTAGGTTTTT 
GG

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wmc(12f7) Plate reference : 12f7
WMC locus number : WMC Length of sequence : 465
Last updated : Mar 25 Aož 1998 at 11:30
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 1 to 462 (complement) show homology to 12h4 bases 1 to 462 (462 in longest run, maxScore = 391), duplicate, 12h4 has precedence. No substantial microsatellite.
PCR Comments :



Length of sequence : 465  Consensus sequence is :

..

GTACCGTGCT TTCAGCGTGG TTGCTGCCAA CGATGTGCTC CTCCTCGGGA 
AAGAGGACGT GGGGAAGCGG AGGCCCCGAC GGTGAGGCGA CCTCACCTCC 
CACCCCGACC TCGGGCGGCG CTACTCCCGG ACCAGACCCA ACCCCAGCGC 
CACTTGCCAT TGCAGCCTCC TGATGCGACA TCCATCGCCT TCTTGAGATC 
TGGCGGCCAC CTGCGCACCA AGTGTTCGGT CAATCGACCC AACAAACAGG 
AGGTAATTCG CCACCTCCTC GTCGAGATTC TTTGACGGCC ACGCGCCGCC 
GCCTGCCGCC ATGTTGCCAT CCCTGTCGAG CTTCTTTGGT CACGTGTCAC 
GTCGCTATCT CTTGACACGC CCGCACACCG TTGCCGCCGT CAACAGGTTT 
TCCGCCCCGC TTTCCCCAGC CATATCATAT CACACTGCGG TTAATCACTT 
CCGATCCTCG TGTAC

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wmc(12g7) Plate reference : 12g7
WMC locus number : WMC Length of sequence : 266
Last updated : Mar 25 Aož 1998 at 11:30
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends NB 5' linker corrupted. Bases 1 to 266 show homology to 8g10 bases 1 to 266 (266 in longest run, maxScore = 227), duplicate, 8g10 has precedence. No substantial microsatellite.
PCR Comments :



Length of sequence : 266  Consensus sequence is :

..

GTACTTTGTG ATTACAATAA AAGGTGATGA AACTTTCCTA GAAGAATACT 
CACTGTAATA TTCTTCAGTA TGTCTTCAGT CTGTACGTTC ACTATGAAGT 
GTGAATGTAA TCTCCGATTT TTTTTAGATT TTCTCCGTAC GTTCAGGAGC 
AGGAGGTGGT CGAAGGAACG TAGGGTAGTA GCCGATCTTA CCAGGTCGCC 
GCCATCGGGG TGATCGAGGT CGCCGTCGTC GTAGCCGCCG CAGATCGCCG 
CCGCCACCGC AGGTAC

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wmc(12h7) Plate reference : 12h7
WMC locus number : WMC Length of sequence : 266
Last updated : Tue, Mar 3, 1998 at 12:15 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. NB 5' linker corrupted. Bases 93 to 136 show homology to 6d5 bases 258 to 301 (44 in longest run, maxScore = 114). No substantial microsatellite.
PCR Comments :



Length of sequence : 266  Consensus sequence is :

..

GTACAAAATT TCACACAGGC GACACAATCA GGGTGGAAGC AGTGGATCGC 
TACGGGTAGG GCTATGTTGA AACCAACGAA CTCATACATA ACGGTTCATC 
GTCTTTATCA ATGACGGGGA AATATCTTGA ATGTTGCGCA AAGAAAACAG 
ACAGACAACA CAATAAGTTC TACTAGCACA TTAAGTTGAC GTCGCCGTCG 
ACCGGGAGGC GGAACCGGTT TTGCTGCGGG TCCACTTCGG TGGCGGTCAC 
CGTCTTCTCG TCGATG

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wmc365(12a8) Plate reference : 12a8
WMC locus number : WMC365 Length of sequence : 345
Last updated : Mar 17 Nov 1998 at 11:02
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at both ends NB 5' linker corrupted. (CT)29 181 to 238.
PCR Comments : tested 51C anneal, no product.
Forward primer details :
AAAcAccAAggTTcAAcTcTcA at 159 length 22 bases, Tm 60
Reverse primer details :
gggAAAcTcAAcAAgTcTAcgA at 291 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 154 bases.



Length of sequence : 345  Consensus sequence is :

..

AGTGTGCCTT GTGTCTAGCA CTTCACCTAT CGTTTGATAG ATGTCCAGCT 
AAACTCTACC TTTCACTCAT AAAAAAGGTA AACTCCACCA TCCAAGTGAA 
GCATGATTAC CCATCCAACA TGAAACAAAC CAAACAGGAA AAATAATGTC 
CAACCGAGAA ACACCAAGGT TCAACTCTCA CTCTCTCTCT CTCTCTCTCT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTAC ATTCATCCAT 
GAGATAATTT TACTTTATCA TATTTCTGGT ATTTTCCTTT TCGTAGACTT 
GTTGAGTTTC CCTTTTTTCC ATTCTATGAA CATTGTTAAC TACAT

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wmc(12b8) Plate reference : 12b8
WMC locus number : WMC Length of sequence : 275
Last updated : Mon, Mar 16, 1998 at 4:25 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. NB 5' linker corrupted. (CAA)9 2 to 28, (CAA)8 32 to 55, (CAA)15 65 to 109, (CAA)50 125 to 274. No room for primer.
PCR Comments :



Length of sequence : 275  Consensus sequence is :

..

ACAACAACAA CAACAACAAC AACAACAACA GCAACAACAA CAACAACAAC 
AACAACACCA ACAGCAACAA CAACAACAAC AACAACAACA ACAACAACAA 
CAACAACAAC ACCAACAACA ACACCAACAA CAACAACAAC AACAACAACA 
ACAACAACAA CAACAACAAC AACAACAACA ACAACAACAA CAACAACAAC 
AACAACAACA ACAACAACAA CAACAACAAC AACAACAACA ACAACAACAA 
CAACAACAAC AACAACAACA ACAAC

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wmc(12c8) Plate reference : 12c8
WMC locus number : WMC Length of sequence : 423
Last updated : Mar 25 Aož 1998 at 11:39
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)25 365 to 414. No room for primer.
PCR Comments :



Length of sequence : 423  Consensus sequence is :

..

ATAGGGAGGC AATCAAAAGT TCCTTGTTTC TTTCATCCAG GAGTCTACCA 
AAATCCAGCA TTTATGTGAA AGGTGTATGC TACATGCGAC CAACAAAAAA 
AAGCTCTTTT TGTGCAGCCG ATGAATGGGC TACTATCCAC TATTTATCTA 
AATAGTTCGT TTCATGTTTC TTATGGTTCA GCCAGTTACC AGTGCACTGT 
TATACTCATT GATGCCGGAT CAAACTAGGA GACTTTTGCT CCAAGAAAAG 
GACTCTTTTG TGGCATCCAA GTTGCTATGT AATAATATAA TTTTTCCCAC 
TATGTCTGAA ACTAACCAAG CCTATCACCA GCATGCTCGT TGGTTTTATA 
TGGTTATTGT GTTTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGCCCCG TAC

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wmc366(12d8.1) Plate reference : 12d8.1
WMC locus number : WMC366 Length of sequence : 217
Last updated : Mar 25 Aož 1998 at 12:04
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Multiple inserts (2) in this clone. This is the first insert. Bases 43 to 60 (complement) show homology to 9d4 bases 379 to 396 (18 in longest run, maxScore = 28). (CA)12 121 to 144, (CA)6 174 to 185.
PCR Comments : 51C anneal, smear.
Forward primer details :
TAccTcTcTAcgATgAAgcc at 61 length 20 bases, Tm 56
Reverse primer details :
TggAgTcTTAgTgTggTgTT at 151 length 20 bases, Tm 55
Expected size of amplified fragment (Chinese Spring=) 110 bases.



Length of sequence : 217  Consensus sequence is :

..

CCTCTTTACG AGCCTCTTCT CTCTCGCACC CCCCTCGTTG AAGGCCTCTG 
CCATACACAT TACCTCTCTA CGATGAAGCC ACACACACTT AGATTACTTT 
CCCCACTGAA CACTCCGCTT CACACACACA CACACACACA CACAAACACA 
AACACCACAC TAAGACTCCA TTTCACACAC ACACACCACT ACAGAAAAAA 
TACACTTCTG TGATGAT

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wmc367(12d8.2) Plate reference : 12d8.2
WMC locus number : WMC367 Length of sequence : 165
Last updated : Mar 25 Aož 1998 at 12:16
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at 5' and and to Rsa1 at 3' end. Multiple inserts (2) in this clone. This is the second insert. Small microsatellite (GCC)5 125 to 139.
PCR Comments : 61C anneal, product.
Forward primer details :
cTgAcgTTgATgggccAcTATT at 8 length 22 bases, Tm 65
Reverse primer details :
gTggTggAAgAggAAggAgAgg at 140 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 154 bases.



Length of sequence : 165  Consensus sequence is :

..

GGCTAGGCTG ACGTTGATGG GCCACTATTC CCGTAGCAAA ATCGGAACTC 
TCTGGTTGAT TTGGGATTTC GGGTCTGTGG TTGGCCATCC CCGCTGCTTC 
CACCCTGTTT GCTCAGCCGC CGCTGCCGCC GCCGCCGCCC CTCTCCTTCC 
TCTTCCACCA CGTAC

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wmc(12e8) Plate reference : 12e8
WMC locus number : WMC Length of sequence : 213
Last updated : Tue, Mar 3, 1998 at 12:25 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 42 to 213 show homology to 12f9 bases 37 to 208 (172 in longest run, maxScore = 154). No substantial microsatellite.
PCR Comments :



Length of sequence : 213  Consensus sequence is :

..

GTACGGACCA CAACGACAAA AATCCGCAGC CGCCGCGGAA CCGCCTTGCC 
ACGCCTATAA AAGGAGGCCC CGAGCTTCCC CGAGCACGCA GGCAACCTCA 
GGCCACCCCA ATGGCACTCC CCTAGCTCGC ATTCGACCCG GGGATGCCTC 
CTTCCTCATC TCCGGCAACT CCTGCCGCCG CCACCGCCCC GACCAATCTG 
GCACCTCCAG TAC

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wmc(12f8) Plate reference : 12f8
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Mar 3, 1998 at 12:27 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : No insert in this clone.
PCR Comments :
No sequence available for this clone
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wmc(12g8.1) Plate reference : 12g8.1
WMC locus number : WMC Length of sequence : 152
Last updated : Mar 29 DŽc 1998 at 9:38
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Multiple inserts in this clone. This is the first insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite. Bases 80 to 148 show homology to 25a3 bases 1 to 69 (complement) (69 in longest run, maxScore = 57). Duplicate with 25a3, 12g8.1 has priority.
PCR Comments :



Length of sequence : 152  Consensus sequence is :

..

GTACCAATAT TTGTATGAAA CTATATTATG TCGTTGACTC ACGATTTGCA 
GTTCAGTTAG ACACAACGAC TCAATATTTC ATACAATCTA TCGTTGGGCT 
ACACATCTGA ACTTTATTTT CATGCATTGT TACAATTTCA CTACAGGAAA 
AC

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wmc(12g8.2) Plate reference : 12g8.2
WMC locus number : WMC Length of sequence : 261
Last updated : Tue, Mar 3, 1998 at 12:33 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Multiple inserts in this clone. This is the second insert. Trimmed toinsert at 5' end and to Rsa1 at 3' end. Bases 121 to 152 (complement) show homology to 8a9 bases 104 to 135 (32 in longest run, maxScore = 76). No substantial microsatellite.
PCR Comments :



Length of sequence : 261  Consensus sequence is :

..

CTTACACTTA GCAGTAGCGC TTTATCTGCA AAGCGCTACT GGTAGTCCAA 
CTTAGCTGTA GCGCGTAATA GTGCAGAGCG CTACTGCTAT TCAGATTAGC 
AGTAGGGCGT TTCGGCAAAA GCGCTACAAC TATCCCTACC TTATCCCCAC 
GCACGCAACC GACCCTCTCT CTCACTCACA CTCTCACTCT CGCCCGCGCG 
CCGATAACCA TCATCGTGCG TCGTCGCCGC CGCCGCCTTT GTCCGTCCGC 
CGCCGAGGTA C

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wmc(12h8) Plate reference : 12h8
WMC locus number : WMC Length of sequence : 6
Last updated : Tue, Mar 3, 1998 at 12:33 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : No insert in this clone.
PCR Comments :
No sequence available for this clone
Go back to index

wmc368(12a9) Plate reference : 12a9
WMC locus number : WMC368 Length of sequence : 493
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimed to Rsa1 at both ends. Bases 461 to 481 (complement) show homology to 11h2 bases 90 to 110 (21 in longest run, maxScore = 113). (CA)14 233 to 260. Forward primer has no homology to 11h2, reverse primer has 2 mismatches. Bases 372 to 404 show homology to 22g11 bases 340 to 372 (33 in longest run, maxScore = 131). Bases 404 to 447 show homology to 21a9 bases 95 to 138 (44 in longest run, maxScore = 113).
PCR Comments : 61C anneal, smear.
Forward primer details :
TTTAgcATgTggcTTTccgTc at 163 length 21 bases, Tm 64
Reverse primer details :
AgTggTcgccATATAgccTTg at 307 length 21 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 165 bases.



Length of sequence : 493  Consensus sequence is :

..

GTACACAACA ACCATCTCTC TCGATTCGTC CGTCCCTCGC ACGATCGCAT 
GTAACACATG CATCAACGCA CACATTTTGA CACCCTCACC CGGCCCCTGC 
TCACCTCAAG GCCCGCACAT CGCTCTCTAT CTCGCACGCA CAATCTCTTT 
GTGAAAACCC TATTTAGCAT GTGGCTTTCC GTCGCATACA CACACAGTTC 
TCTCCATAGG TTTGATTTCT CTCAAAACCT GACACACACA CACACACACA 
CACACACACA CCGCACACAC ACTCCCCCTC CCACAGGCAC ACAATTCATC 
CCTATCCAAG GCTATATGGC GACCACTCTG GCTTGTGCTT CTCTGCACCC 
CAGCATGCAC AACAACTCAA CCTTCAAAGA GGGCATCGAG GAGATCGAAG 
ACGGTGACCA CACTGCACTA GAGAATGCGG GGCCATTTGG AAGCAGGCTG 
ATGTGACGAC GGCTGACTGA CTCCTCCCCC CTCTCTCCCG TAC

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wmc(12b9) Plate reference : 12b9
WMC locus number : WMC Length of sequence : 276
Last updated : Mar 25 Aož 1998 at 14:03
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)10 251 to 270. No room for primer.
PCR Comments :



Length of sequence : 276  Consensus sequence is :

..

GTACTAGTCC TTGGGAGCCT AGAGTGAACC TAGGAGATTA GCTGGATAGA 
CTTAGTTGTG TTAGTGGACG GTAGAAGTGG TCATGGCATA GTGGGCAGCG 
TGAGTTGTGC GGCTTGAGTG CTGGTCCCTG ATGTATATAT ACGTGTTGCA 
TATTGAGTAA TGAAAAAGGC TGAGAGGGTT GAAGGAAAAA CCATGTAGCA 
CATGTGTTCT CACCGGGGGA AGAAGCAAAG CCTTTCCTCT CCTTGGTGAA 
GTGTGTGTGT GTGTGTGTGT CGTGTG

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wmc(12c9) Plate reference : 12c9
WMC locus number : WMC Length of sequence : 375
Last updated : Tue, Mar 3, 1998 at 12:47 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 375  Consensus sequence is :

..

GTACAGCGAC CCGAGGATCG AACATGACGA CGATGATGAA GTAGCAGCAT 
ACACCACAAG AAAAAAGCAG GACCACCCTA CCTAAAGGAC GTCCGTTCCA 
TAGAAGAACT CCATTTGCGA AAAAGAAGGG CAAGAAGATT GTGAACAGAT 
AGCTAGCTAA GATCGATTGT ATTTAAATCG TAGACTTCAT TTCTCGATTG 
TATTTCATGG GCACCTTTTG AACTATCATG AATATTTTTT AATTTCATGG 
ACACTCGATC TCGATCCCCC TCCATCTCGA TCGCTATCCC ACCTCGCCAG 
ATCCGGTCCA CCGGCCGCCG CCGCCGACCG CCCGCACCCT CTCGATTCCC 
CTACTCAACC GCCGCCGCCG ACCCC

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wmc369(12d9) Plate reference : 12d9
WMC locus number : WMC369 Length of sequence : 337
Last updated : Tue, Mar 3, 1998 at 12:51 pm
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Interspersed CA microsatellite.
PCR Comments : 61C anneal, product.
Forward primer details :
gcAcAAcAAcAcccAAgcAAcT at 95 length 22 bases, Tm 66
Reverse primer details :
TgcccTccATATcTTcTTgAcg at 311 length 65 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 238 bases.



Length of sequence : 337  Consensus sequence is :

..

CTCGCACCCG ACGATGCCTC GGCTCACCAC CGACACAAGC ATACACACCA 
GCACCCGCAA AAAGAAGACA AACGAGCCCA CAAGGCGCAA GCACGCACAA 
CAACACCCAA GCAACTGGTC ACAAGCTCTC TTCCCGGGTT CGCTGACAGC 
CACCAACGCA GCGGCGGTAG GACGGCCAGG TGGCATCGCG GGGCATCCCA 
GTGAGCTCCA ACGTCCCTGC ACACGACACA CACACATCGA CGACCTCACA 
AACGACACAC ACACACACCC CACCCAACAC AAACACGCAC ACCACACCAT 
GCGAATGCCG CGTCAAGAAG ATATGGAGGG CATGTAC

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wmc(12e9) Plate reference : 12e9
WMC locus number : WMC Length of sequence : 261
Last updated : Mer 26 Aož 1998 at 15:38
Owner : Mohler, Munich Date at Agrogene : 2.3.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 5' end. (GT)14 232 to 259. No room for primer.
PCR Comments :



Length of sequence : 261  Consensus sequence is :

..

GCGCGCACCG AGATGTCGGG CTTCGTCATC GCCCGGAACG CCGAAGCTGA 
CGCCGGCTCC AGCGCTCCCG GCCCCGCCGC CGCGTCGTTG CGCTCCATTA 
CGCGCTCCAG GAGCGTGGAG AGGACGTCCA CCACCCGCGG CATGTCCGTT 
GCCGGTAGAT CCTCCTCCTC AGCACTGCCC ATGCTGGCTT CTCGAATCTC 
GATCGCCGGC TCCCTTGGAT GCTGTGCGCC TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTA C

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