Wheat microsatellite WMC 25/06/01

wmc(10g8) Plate reference : 10g8
WMC locus number : WMC Length of sequence : 119
Last updated : Jeu 27 Aož 1998 at 12:04
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end , 3' end not located. Bases 4 to 40 show homology to 1C2 bases 856 to 892 (37 in longest run, maxScore = 24). No substantial microsatellite.
PCR Comments :



Length of sequence : 119  Consensus sequence is :

..

GTACATAGAC AGATGCCGAT ACGTTGTGyA TACATGAGAG AACGGTCTTC 
TA.TTTACTT GTGTGTGTG
(break)
TGAGAGAGAG AG.....GAG CAy....CCG TAACrsA.AC AmmmCAACCA

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wmc383(10h8) Plate reference : 10h8
WMC locus number : WMC383 Length of sequence : 401
Last updated : Mar 30 mai 2000 at 9:54
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)15 315 to 344.
PCR Comments : 61C anneal, product.
Forward primer details :
AgccAAAcAcTcgcATcAcg at 169 length 20 bases, Tm 66
Reverse primer details :
gAAccccTTgAcAgTTcgcc at 380 length 20 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 231 bases.



Length of sequence : 401  Consensus sequence is :

..

GTACCACAGA ATGAAGGAAA CAGACCTCCT ACAATCGAAA CGGGGGTCCT 
GTGGATCGGG AGGGGTGTCG CGTACAAATC CAGAGAGCCT AACCTACAAG 
CACACCAAGA CCCAGCCTCT ACACTGCCGA TGGGAAAGTT GTTGGCGCCA 
CAACGGAGAG GGAGGGGAAG CCaAACACTC GCATCACGGA GTCGCCGCCC 
aAATCACTTG AgGTGCaAAA AAAGACATAA AGCaAACgGG AGAAAATCTG 
CAAAAagGGT TGTGGAGCCC TAGCTTGCGC tTTTGCGCAa gGAGCCTAGa 
ATCCCTCTCT CTCTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTTTGACA 
CCCCCGATAC GACCGTAGTT TTATTCTAgG GCGAACTGTC AAGGGGTTCC 
C

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wmc(10a9.1) Plate reference : 10a9.1
WMC locus number : WMC Length of sequence : 250
Last updated : Jeu 27 Aož 1998 at 15:52
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Apparently multiple inserts in this clone, joined by a corrupted linker. This is the first insert. Trimmed to Rsa1 at 3' end, 5' end not located. Bases 219 to 250 show homology to 6e12 bases 389 to 420 (32 in longest run, maxScore = 91). (CA)10 84 to 103. Duplicate, 6e12 has precedence.
PCR Comments :



Length of sequence : 250  Consensus sequence is :

..

ACTACCGAGC ATCAGCAATG CATATGCTGA AGATCTATTA CGAGCATCGA 
CCGATGGATC TCTCTTTCTA TCTCTCTCTC TCTCACACAC ACACACACAC 
ACACGCACAC ACACACGCGC GCGCACACAC rCATCAAGTG GGACCCGTTG 
AATTATTTAT TTGGTCGTGA CAGTTTTTAA TTAGGTTCCA CTGTAATCTA 
ACGTTTTTCT TAAGTTACTA ATTGAGCTCA GTGACTTCAA AAAGTTGTAC

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wmc(10a9.2) Plate reference : 10a9.2
WMC locus number : WMC Length of sequence : 171
Last updated : Jeu 27 Aož 1998 at 12:05
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Apparently multiple inserts in this clone, joined by a corrupted linker. This is the second insert. Trimed to insert at 5' end, 3' end not located. Bases 85 to 147 show homology to 5e12 bases 586 to 648 (63 in longest run, maxScore = 53). No substantial microsatellite.
PCR Comments :



Length of sequence : 171  Consensus sequence is :

..

GGCCTCGAGG CGATGATkmC TCTAGAGkmT CCCTCAGGCG CGTGCGTGTT 
GTTGCGTGTG TGTGCGCGTG CGTGTGTTGG TGCGTGTGTA TGTGTTGCTG 
CGTGTGTGCT CCTGCCCTCG CATTGATGCT GCGTGTGTGC GCTATTGCCC 
CCTACACACA TACCTAGTCA C

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wmc(10b9) Plate reference : 10b9
WMC locus number : WMC Length of sequence : 163
Last updated : Jeu 27 Aož 1998 at 12:05
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert. Bases 55 to 74 (complement) show homology to 4d10 bases 111 to 130 (20 in longest run, maxScore = 36). (TG)5 67 to 76, (GT)7 82 to 95, (GT)7 104 to 117. Duplicate, 4d10 has precedence.
PCR Comments :



Length of sequence : 163  Consensus sequence is :

..

GTACATACTC CATTGATCTC ACTTCCTGTG GCTTCCCACT CCCACGATAT 
TCCAATTCTA TGAACCTGTG TGTGTGCGTG CGTGTGTGTG TGTGTTTAAC 
AGCGTGTGTG TGTGTGTTAG AGAGAGCGAC ACATTGACCT TACAGAGAGA 
TGGTGTGTAG TAC

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wmc(10c9) Plate reference : 10c9
WMC locus number : WMC Length of sequence : 437
Last updated : Lun 2 Nov 1998 at 9:57
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Bases 231 to 266 show homology to 13c5 bases 334 to 369 (36 in longest run, maxScore = 148). No substantial microsatellite. Bases 220 to 267 show homology to 10b6 bases 77 to 124 (48 in longest run, maxScore = 121).
PCR Comments :



Length of sequence : 437  Consensus sequence is :

..

CATAGCATTG GACAGmGAGG caGCAGGGTC GGrrTAGCGG ATTCGACTTA 
CTACTAGAAA GGTAGGGTTT GTGCGATTTG ATGGCGCGTT ACTGCTGGAA 
AGACGGGGTC TTGTGATATG ACTACTGACT ATCTCTACTC TTATAAAAAA 
CTTAGTTGGT GATGATGGTG TGCCTGCCAT TCTGCAATAT AGGCTGTCCG 
ATTTATATCT GACGGATAGG AAGGAAACAA TGGCAAtTTT GCAAAAAGAT 
ACCCACACCC CTCTCCACAT TTGCAAATAA GGCCTTCCCT TGTTCATCCT 
TTTCTCCCAC AACATAAACT ACTCATACAA ATGCATACTG ATGTTCCGTG 
CAACGCATGG GCATCTTGCA TGTAGACATG ATTACTGGCG CGACGACCGG 
GTTGATGCCC CCCATCGTTC CCACTCTAAT GTGGTAC

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wmc384(10d9.1) Plate reference : 10d9.1
WMC locus number : WMC384 Length of sequence : 341
Last updated : Jeu 27 Aož 1998 at 18:01
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Apparently multple inserts in this clone, this is the first insert. 5' end not located, trimmed to insert at 3' end. Bases 21 to 36 show homology to 10e8 bases 16 to 31 (16 in longest run, maxScore = 32). (CA)12 116 to 139, (CA)15 151 to 180.
PCR Comments : 61C anneal, complex product.
Forward primer details :
TggATAgTTTAccAccAAAgccA at 60 length 23 bases, Tm 65
Reverse primer details :
AcATTccTgcATTcATgcTcAc at 197 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 159 bases.



Length of sequence : 341  Consensus sequence is :

..

TGGACTAACT ACGTGGAGAT AGGTAAGGTG ACTAAGACTG CATTATTTCT 
GCATTACTAT GGATAGTTTA CCACCAAAGC CACATGACAC GATTATnTTG 
AAATCCTAAT GACTCCACAC ACACACACAC ACACACACAC CTAATGACTC 
CACACACACA CACACACACA CACACACACA AAACAGCGGC ATGCTGGTGA 
GCATGAATGC AGGAATGTAT AAAGGATGCA AGGATTATAT ATCCCTAAAC 
TTCTAGAACA TAACTAAGAT CGGGAGTTCC GGCCATCGCA AGCCTCTATA 
GCCACCAAAA ACCAATCAGG ACCCTATTCC GGCACCTAnC C

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wmc385(10d9.2) Plate reference : 10d9.2
WMC locus number : WMC385 Length of sequence : 219
Last updated : Jeu 27 Aož 1998 at 18:10
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Apparently multiple inserts in this clone, this is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)26 127 to 178.
PCR Comments : 51C anneal, complex product.
Forward primer details :
gAAATggcAATggAcTcAc at 73 length 19 bases, Tm 59
Reverse primer details :
ccATcATATcccTAgcAcAA at 200 length 20 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 146 bases.



Length of sequence : 219  Consensus sequence is :

..

GTACATGGTT GATTTGAGGA GGCCGTGATG GTGATGGTGT CCCGGTGCGT 
GCATACGGAG TTGGGTTTCA GGGAAATGGC AATGGACTCA CAAATTAAGT 
CTTGTTCAGT TGCGAGTCGT GGCAAAGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGC CTGTTATAAA CTGTTATTAT 
TGTGCTAGGG ATATGATGG

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wmc(10e9) Plate reference : 10e9
WMC locus number : WMC Length of sequence : 211
Last updated : Jeu 27 Aož 1998 at 12:05
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 177 to 202 show homology to 7e1 bases 134 to 159 (26 in longest run, maxScore = 63). No substantial microsatellite. Duplicate, 7e1 has precedence.
PCR Comments :



Length of sequence : 211  Consensus sequence is :

..

GTACCGTCGT CAGGGTCGTT GGGAAGGCAC TCCTCCACTC GTCGACGTCG 
ATCTCCTCGT CCTCAAGGCC CACCTCGACG CCCTCCGCGC CGCCGCCACC 
GTCGCCGCCA CCTTCGACCT CGTCATCAAA GGAGAGCACA ATAACCTCGC 
CGCCCTCCAC ACCGCCGTCC TCACCACCTT CGACCTCGTC ATCGGAGGAG 
AGCGCGATAA C

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wmc386(10f9) Plate reference : 10f9
WMC locus number : WMC386 Length of sequence : 223
Last updated : Jeu 27 Aož 1998 at 18:20
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. (GT)22 102 to 145.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Forward primer details :
ATcAcTgAAAcgAAATgAgcgg at 16 length 22 bases, Tm 65
Reverse primer details :
TggTTggcggTTTTTcTcTAcA at 159 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 165 bases.



Length of sequence : 223  Consensus sequence is :

..

TGACATTAAC CATATATCAC TGAAACGAAA TGAGCGGTGG CTGGGACACT 
AGCATCATTT ATCTGCATTA CAATGGGTGA AAACTAGGGA TATATATATA 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGACTT 
CTTATCAATG TAGAGaAAAA CCGCCAACCA AGAGATTATG GCACGATAAA 
AAAAAGGGCA GnCCGGTGCA TGT

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wmc387(10g9) Plate reference : 10g9
WMC locus number : WMC387 Length of sequence : 408
Last updated : Ven 28 Aož 1998 at 9:27
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 70 to 102 (complement) show homology to 14c4 bases 346 to 378 (33 in longest run, maxScore = 68). (CA)12 116 to 139.
PCR Comments : 51C anneal, very complex product.
Forward primer details :
cATTTTgAcAcccAcAcTcg at 14 length 20 bases, Tm 62
Reverse primer details :
cTggATccccTcTTcgcTAT at 189 length 20 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 195 bases.



Length of sequence : 408  Consensus sequence is :

..

GCATCAACGC GCACATTTTG ACACCCACAC TCGGCCCCTA CCCACCTCAA 
GGCCCGCGCA ATCTCTTGCT GAATACCCAT TTCTCTCCTT AGGTTTGTTT 
TCGCTCAATA TCTGACACAC ACACACACAC ACACACACAC TGATGCTCCT 
TCTATCTCCT GTCGTGCAGG TTGCCGCgga tTTTTGCCAT AGCgaAGAGG 
GGATCCAGcC GGCaCGCTGT GTCTCCCGAT AAACATGCCT AATTTTATAT 
GCAGAGGTAG CACTGATCTA CTTGCCCCCT TCAAAAGGCC ATATGTGTTG 
CTTTGTTTTA CTTCTCTTTC ATATCCAGTT ATCTAGAAAT TCCCTGCTAA 
CCTCCTGGCA CTTAAATACG ATTAGGTATA ATGTGAAGnA TTTGCTTGAC 
AGAGGTAC

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wmc388(10h9) Plate reference : 10h9
WMC locus number : WMC388 Length of sequence : 354
Last updated : Ven 28 Aož 1998 at 9:37
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : 5' end not located, trimmed to insert at 3' end. (GT)5 140 to 149, (GT)4 152 to 159, (GT)7 162 to 175.
PCR Comments : 61C anneal, polymorphic on acrylamide, though complex.
Forward primer details :
TgTgcggAATgATTcAATcTgT at 47 length 22 bases, Tm 64
Reverse primer details :
ggccATTAgAcTgcAATggTTT at 186 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 161 bases.



Length of sequence : 354  Consensus sequence is :

..

GCCTGTGCGG TGAGTAATCG ACAGCAGCTT ATTTTATCAA AAAGTTTGTG 
CGGAATGATT CAATCTGTTT TTGTCATATA TGTAGTGTGT GTCACATTGG 
ACTGACCATT TTACATCTGC TGCTGGTCTC TGTGCGCATG TGTGTGTGTG 
CGTGTGTGTA TGTGTGTGTG TGTGTTCTAC ATGGTAAACC ATTGCAGTCT 
AATGGCCGGT CGAAGATGTG GGTGCAATGC CATGATGCGG CTGCTGAGAT 
CGGATGATTG TGGCTGGTAT GTGTGCGAGC ACAGGTCAGA GCACAACCAT 
GATCTATCAG CTAACCGTGG GnATAAATGC ATTGGAAGTC AGCTAGCTCG 
GCAC

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wmc389(10a10) Plate reference : 10a10
WMC locus number : WMC389 Length of sequence : 209
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 177 to 205 show homology to 2h10 bases 449 to 477 (29 in longest run, maxScore = 36). (GT)27 28 to 81, (GT)9 136 to 153. Bases 127 to 142 show homology to 22d1 bases 108 to 123 (complement) (16 in longest run, maxScore = 25).
PCR Comments : 61C anneal, smear.
Forward primer details :
gTgcggggTTAgAcTcAcAAA at 3 length 21 bases, Tm 64
Reverse primer details :
cAAcTgggggTTcTcAAcAAA at 155 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 173 bases.



Length of sequence : 209  Consensus sequence is :

..

AGGTGCGGGG TTAGACTCAC AAACATTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TTATTTTTAT CAAAGTGTCC 
GCAGTTGAAT GTCAACCATC GACTCACAAC TAGGGGTGTG TGTGTGTGTG 
TGTATTTGTT GAGAACCCCC AGTTGTAAGT TGACCATCGA CTCGCAACTA 
GGGGGGTAC

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wmc390(10b10) Plate reference : 10b10
WMC locus number : WMC390 Length of sequence : 517
Last updated : Ven 28 Aož 1998 at 10:13
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end , 3' end not located. Bases 1 to 40 (complement) show homology to 8a12 bases 123 to 162 (40 in longest run, maxScore = 48). (GT)16 84 to 115.
PCR Comments : 61C anneal, product.
Forward primer details :
TgcAAATgccATggTTgTAgTA at 7 length 22 bases, Tm 63
Reverse primer details :
cAgAcgggTTgcTgcTAATAAA at 169 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 184 bases.



Length of sequence : 517  Consensus sequence is :

..

GGTGTGTGCA AATGCCATGG TTGTAGTAGC CACCTATGTA .AGTGGATGT 
TTAATTTGGk TTATGCCAGC ATGTCCT.TA TAAGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTCTATA TATATGTTGT cTCTATATGC AAcTCCaCAT 
GGCACAAaCA CACACGTCTT tATTAGCAGC AACcCGTCTG TGTTATTAcT 
CGGTCTcTCA CACACATTcT CTGATTACAG ACtCTAACAA GCTAGAgAGA 
CAATGCAAGA TGCAACcTAC ATCACAGTCC CAAGTTAAAT GTGTCTTATG 
GGTCAGTGAT CGTTGCAGGT ATAAGTTGTA AcGTACGCAG ATGCAATTCA 
ACATAATCAG AAGCAATACA AACTAGACAT CATACGGAAA ACGCAACCAC 
ATATAACAGT TCCAAGTTAG ATCAAGCACC TGTGATGGTG GAGTAGGGGA 
GATGTGCTCA TCATGTTAGT CCaACGCGCC TCGAGGGATC CTCTAGAGTC 
GACCTGCAbG CATGCAA

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wmc(10c10) Plate reference : 10c10
WMC locus number : WMC Length of sequence : 171
Last updated : Ven 28 Aož 1998 at 10:18
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GA)17 10 to 43. No room for primer.
PCR Comments :



Length of sequence : 171  Consensus sequence is :

..

GAGCTGCTGG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGCTAGTC 
AGAAAGGCtA AGGGCTGAGC TAACGGTAGC GACCTCCAGA CCTCTTTCTC 
TTCTCTcTGt TTTACCCGTG TATCTCTTGT GATGGACCAC CATATATGCG 
GCTTCATTGT ATTGTGGTTT G

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wmc(10d10) Plate reference : 10d10
WMC locus number : WMC Length of sequence : 373
Last updated : Mer 14 juin 2000 at 10:34
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 233 to 281 (complement) show homology to 5a4 bases 178 to 226 (49 in longest run, maxScore = 118). (GT)10 353 to 372, (CT)14 321 to 348. No room for primer. Bases 258 to 320 show homology to 25b8 bases 84 to 146 (complement) (63 in longest run, maxScore = 63). Bases 103 to 124 show homology to TW1a02 bases 461 to 482 (22 in longest run, maxScore = 23).
PCR Comments :



Length of sequence : 373  Consensus sequence is :

..

GTACTAGACT TAATATTCAT GTTTCAGTTT CATGTTCTTT TTAAATATTG 
AACTGAGGAC CATGGATGTC TTACATTTCA CTCCCTTTGT TCCTAAATAT 
TAGTCTTTTT AGAGGTTTCA AATGGACTGG CACATACGGA TGTATATAGA 
CATATTTTAG AGTCTAGATT CACTCATTTT GCTCCATATG TAGTCACTTG 
TTGAACTCTC TAAAAGACTA ATATTTAGGA ATAGrAgGAG TATTTTTGAA 
TTCGTGTCAT ACATGCATGG TTTGGAAAAA TAGATGCACT ATACTTATTG 
GATTGTTGTT GTGTTCGTGT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCC 
CTGTGTGTGT GTGTGTGTGT GTG

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wmc(10e10) Plate reference : 10e10
WMC locus number : WMC Length of sequence : 798
Last updated : Jeu 27 Aož 1998 at 12:05
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert. Bases 471 to 488 show homology to 9a6 bases 493 to 510 (18 in longest run, maxScore = 20). No substantial microsatellite.
PCR Comments :



Length of sequence : 798  Consensus sequence is :

..

GTACTAACAA ATTCCTGGCT CCAAGGCTGC TGCGCTTGCG CATAGGTCGC 
CGATGAAGCT TCTCCCTGTA GGTCCGGATC CTAACAGAAA CCATCGGATC 
CTAACGAAAC CAACCCACCA CCGGGGCTCA CGCCGTCGTC GTGACGCTTC 
CGCCTATTAA AGGTGACCGC CGCCGCCGCC GCGCACAGCC CACTCCACTA 
CGACAGGAAA GAAACGCAAG GGAAATGTCA ACCAGCACGT TTGCAACCTC 
GGCCCGCGCG CTCCAGCACA TCATTTCAAC AGCCGGTGCC TCCCGCAGAG 
CGCCCACGAG CCCTCGcCGT CCGTCGTCTC TACGCTTCGC CGGCCGAGGA 
GGCGCCGCCG CTTGCAGGTC CCCGAGCCTC CTTAATTAAG GTCATCCCGC 
TGCAAGCCGG ACGTCTCAGC GGCGGTCGTC TACAgcGGGT GAAGCTGGTa 
GACCCCGGGa rggcCGGGaa tTa.gtccaC G.cgtGgact taacaytaca 
cacc.atctc tctgtagcgg Tagktcgatg TgktGctstc tytgasacag 
acatgktgct raaCacgcac a.....acac asacacrcag GaTCATAGAA 
TAGGAATATC GTGTGAGTTA CTTAGGrAGc GTTTGGATCC TTAGCAAGAA 
CAGTTTATAG CCTAGCTGGG CAAGGTTAGG TGCTTGGAAC TGTTAAAATA 
TGTTAGCTTT TGTGGGCCAT CTAGCTTGGG TGGGCTAGAA AAACCTTGCT 
AGCTCAGGAG AGCCAGATCG CTCGCTTCCG ATGGCAAAAT TTGCGTAC

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wmc(10f10) Plate reference : 10f10
WMC locus number : WMC Length of sequence : 330
Last updated : Lun 2 Nov 1998 at 10:06
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 140 to 215 show homology to 3g9 bases 137 to 212 (76 in longest run, maxScore = 137). Bases 93 to 138 show homology to 10d11 bases 424 to 469 (46 in longest run, maxScore = 60). No substantial microsatellite. Duplicate with 3g9, 3g9 has precedence. Bases 90 to 217 show homology to 24h5 bases 21 to 148 (complement) (128 in longest run, maxScore = 153).
PCR Comments :



Length of sequence : 330  Consensus sequence is :

..

GTACGCCAAC AACGGCGCAA GGCACCGCCG TCGCCGTCGG TCAGTGGCCC 
GACGACACAC AGAGCATGGC AAAGGAGACG CTTCCCACCG CAAGACGCCC 
CCGCCGCCGC CGCGTAATCT AAATTTGCCA TGAAAAACAT TCGGATTGCC 
ATGCTTAAAA TCCGAACGTT TATCATTTCC GTTTATTTTA GATCGATCGT 
CGTATAATTT AAAGTCGGAG TCAGACTGAA CGAAATCTAC GGTTTGATTG 
ATTGAATGTT CTGCTAGAGC CGTATCAAAT TAAATATAAA ACATCATCAA 
ACCACATGTA TTCCTTGTCA TCCAATGACC

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wmc(10g10) Plate reference : 10g10
WMC locus number : WMC Length of sequence : 265
Last updated : Jeu 27 Aož 1998 at 12:06
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 146 to 156 show homology to 5g11 bases 140 to 150 (11 in longest run, maxScore = 21). No substantial microsatellite.
PCR Comments :



Length of sequence : 265  Consensus sequence is :

..

CACGAGGACT AATTAGTGAA GCACAAGAAT GAATGGGAGG AGAACATCAC 
ATAGCTACTG GTCATGACCC ATGGTCCAAG GGAGACTACT CACAACTCAT 
CCGGGAGGCG TCCATGGTGG GGGAGAAGCT CCCGGTGGTG ATCCTCCCCT 
CCAGCAGGGT GCCGAGAAGA GTTCTCCTGG TGCTCCCGAT CTTGGAAGCG 
GCGGCGGCGG CGGAACAGAG ATGAGATCGC GAAAAAGGGT TGCGCCGTCG 
TCGAGGGTTT CGGAC

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wmc391(10h10) Plate reference : 10h10
WMC locus number : WMC391 Length of sequence : 513
Last updated : Jeu 12 Nov 1998 at 14:24
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end , 3' end not located. Bases 127 to 191 (complement) show homology to 3A1 bases 668 to 732 (65 in longest run, maxScore = 124). (CT)16 24 to 55. Bases 127 to 179 show homology to 24c2 bases 124 to 176 (53 in longest run, maxScore = 102), duplicate, 10h10 has priority.
PCR Comments : 51C anneal, smear.
Forward primer details :
gTAccAgTAgcTATgTgTTTgAT at 1 length 23 bases, Tm 55
Reverse primer details :
TTAcAAgAgAgTAgAggggAAg at 124 length 23 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 145 bases.



Length of sequence : 513  Consensus sequence is :

..

GTACCAGTAG CTATGTGTTT GATCTCTCTC TCTCTCTCTC TCTCTCTCTC 
TCTCTTGTTC TTGATATTGC ACAATCTTGA TGTATTGCGA GCTTTGCTAT 
TGTAGTTGGA TCTTATGATG TTTCTTCCCC TCTACTCTCT TGTAATGGAT 
TGAGTTTTCC CTTTGAAGTT ATCTTATCGG ATTGAGTCTT TAAGGATTTG 
AGAACACTTG ATGTATGTCT TACGTGGGAT ACCCGTGGTG ACAATGGGGT 
ATTCTATTGA TACATGCCCC GGAGTCTCCT GAGAGCCGCC CCGGGGCCTC 
ACGGGGGCTC CCTCCCACGT CCTAGTGGCA GCACTTAGCT CCACGCTGGT 
GAGAAGACTA GATTAGGTGC CGGTCGCGGT CATTCATTTG CTTTCTGGAG 
TTGCTTGTAG TTTTCTAGTA ATCTTTCAAT GGATTTGGTA TTCTCGAACA 
CTGGTTCCTC GAAGTTATCT CCTTCTCTTA AGTArkCCAC GCGCCTTmGA 
GGAwTyCAmT GGG

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wmc(10a11) Plate reference : 10a11
WMC locus number : WMC Length of sequence : 293
Last updated : Ven 28 Aož 1998 at 11:27
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Bases 73 to 266 show homology to 8g12 bases 86 to 279 (194 in longest run, maxScore = 168). (CA)19 228 to 265. Duplicate with 8g12, 8g12 has precedence.
PCR Comments :



Length of sequence : 293  Consensus sequence is :

..

GCCTAATAAA TCATGTTACC TTTCCAATAC AGTCAGGGAC CATGCATCTT 
ATGCCTTCAA CAGCTACTGG CtAAAAATAC AAGAAACATG GTGGTTCTTG 
CTACTTCAAT GCAGCTGCAA TGGTCACCGA CCTAAACCCA AGTATGTTCA 
CAACCTAAAC CATCATTAGG AGCATACACT TTTGCAGGTT GAAATTCCAC 
TAGAAAGATC AAACATGCAG AGCACAACAC ACACACACAC ACACACACAC 
ACACACACAC ACACACCACA AGCCCGCACA CACGCACGTG TAC

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wmc392(10b11) Plate reference : 10b11
WMC locus number : WMC392 Length of sequence : 217
Last updated : Ven 28 Aož 1998 at 11:33
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. (CAA)28 109 to 192.
PCR Comments : 51C anneal, smear.
Forward primer details :
cATAgcAccAAggAAgAgT at 47 length 19 bases, Tm 55
Reverse primer details :
gTTgAgTATccAAAcAggg at 199 length 19 bases, Tm 55
Expected size of amplified fragment (Chinese Spring=) 171 bases.



Length of sequence : 217  Consensus sequence is :

..

GAGGCGCGTG AACTAACTTa aTGTCTACAT ATGCAATCTT AGGCTACATA 
GCACCAAGGA AGAGTGTTAC CTTAATGTTC TGAkkATGTC TAGATATACG 
TAGAAGAGCA ACAACAACAA CAACAACAAC AACAACAaCA ACAACAACAA 
CAACAACAAC AACAACAACA ACAACAACAA CAACAACAAC AACAgCAACC 
CTGTTTGGAT ACTCAAC

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wmc(10c11) Plate reference : 10c11
WMC locus number : WMC Length of sequence : 400
Last updated : Thu, Aug 27, 1998 at 11:12 am
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end, 3' end not located. No substantial microsatellite.
PCR Comments :



Length of sequence : 400  Consensus sequence is :

..

GTACTGACTA TCCTCGCATG CGGAGTAACA GCGGCAGAAT TCACCGATTT 
ATAAAGTGGC aGGCGGGGGs GTCCAAtCTC CTTGTCAGAT GAGGCGGCTC 
CTGCGGCGGA AACATGGCAC GCCGGCGGTG GGGGCGTCCA ATCTTCTTGT 
CAGATGAGGC GGCTCCTGCG GCGGAGACAT GGAGCGCCGG cggscsGTGC 
GTTGGGGAGG CGTGGGCGGA GGTCAGAAGA GGAgGGCTGG CAAGGGGGCG 
GCCGGGAGGA GGAAGGATGG TGGGGAGGGA TACCtAtGGG ACCCATCGCG 
CTTCGATTGG CAGACCGTGG CAGAACATTT ACCCTGAGCC CGtTTCGATG 
GGAGAGGCAG ACCACAAAGt TAAtTATTGg aACTCtTCAG ATTTTGTGtt

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wmc(10d11) Plate reference : 10d11
WMC locus number : WMC Length of sequence : 559
Last updated : Jeu 27 Aož 1998 at 12:06
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : 5' end not located, trimmed to insert at 3' end. Bases 424 to 469 show homology to 10f10 bases 93 to 138 (46 in longest run, maxScore = 60). No substantial microsatellite.
PCR Comments :



Length of sequence : 559  Consensus sequence is :

..

TAsACChCCd sTCAAATrrT CGAsACwCwC TCACGAAGGk ACwACACGTC 
TCACGAmGwv CGCsCkTmkA CGACwCTCAr AsGATCCCTC GAGAGmrCGT 
rsACTmwssG AGakmCswCT sTTGGGAggy TyyTCTTGAG skyCTTCGGC 
rGAsmCmCAG tTTGACAcwT GGgcCCACGt TGaAAGcCGG TtgaacCCGG 
tTTTAGcCwT GaAAACACTT TTtcCCTaGT ccGGCtTTGc CACGGCCaAA 
CAGCTTTAGc CAGATTGGTT GGGGTTGGTC TGGGGACACT CACGCATATA 
ATgACCAGAT TCCCCACACT TGAAACAAGT CACGGAACTG ATACGCCAAC 
AACACAGAGC ATAGAGCACA GTCAGTGGCC CGACGACAAA CAGAGCACGA 
CAGAGGAGCC GCTTCCCACC GCGAGACGCC CCCGCCGCCG CCGCGTAATC 
TAAATTTGCC ATGAAAAACT TTyGGATTGA CryGcTrGwC yAGCryGTCA 
ATCCrAAmGT TTTTCATGGC AAATTTAGAT TACGCGGnGG CGGCGGGGGC 
nTCTCGCGG

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wmc(10e11) Plate reference : 10e11
WMC locus number : WMC Length of sequence : 391
Last updated : Jeu 27 Aož 1998 at 15:53
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 298 to 391 show homology to 5b8 bases 293 to 386 (94 in longest run, maxScore = 145). (GT)12 197 to 220. Duplicate, 5b8 has precedence.
PCR Comments :



Length of sequence : 391  Consensus sequence is :

..

GTACTACAGC TGTGCGTGCG CGAGAAGTCT ACATATCGAG GGTCTACCGA 
GCGAGGCACC GAGACACAAT GTTTGAGAGA GAAACCAATT GACAGATTAT 
GATCAGATCG AGTTGTCACC CTTCTGCTGT CATTGTTGGG GGGAGGgAGA 
GAAaGACGTA TCGAGAGTGT GTGCGgTAGG CACAGAGGCA CAACTAGTGT 
GTGTGTGTGT GTGTGTGTGT GATTGAAAGA GGAGTAGATA GATTATTATC 
AAGATCGATG TGCCACCCTA CTACTTCGGT GCCGGGTAGT GTGTGCATGT 
GTATGTTTGA GAGAGAAGCC AGTCGATAGA TTAGTATCAA GATCGAGGTG 
TCACCCTACT CCTTCGGTGT TGGGAAGTGT GGGTGTGTGG G

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wmc(10f11) Plate reference : 10f11
WMC locus number : WMC Length of sequence : 229
Last updated : Jeu 27 Aož 1998 at 15:53
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end, 3' end not located. Bases 130 to 149 show homology to 5c12 bases 173 to 192 (20 in longest run, maxScore = 40). (CAA)8 124 to 147. Duplicate with 5c12, 5c12 has precedence.
PCR Comments :



Length of sequence : 229  Consensus sequence is :

..

GTACAGCAAT ACCAACAACA ACAGCAACAG CAACAGCAAC AGCAACAsCA 
ACAGCAACAG CAACAACAAC AGCAACAACA GCAACAACAG CAACAACAAC 
AGCAACAACA ACArCAACAG AAACAACAAC AACAACAACA ACAACAACAG 
CAGCAACAAC AGCAACAACA GTTACAACAr TAACAGsAkC AACAGACACA 
ACAGTAATAA CAGCAACAAC AGCAACTAC

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wmc(10g11) Plate reference : 10g11
WMC locus number : WMC Length of sequence : 276
Last updated : Thu, Aug 27, 1998 at 11:13 am
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end , 3' end not located. No substantial microsatellite.
PCR Comments :



Length of sequence : 276  Consensus sequence is :

..

GTACGCTGTA GCGCGGGGGC GGCGGCCAAG GTCGAGGTGG GCATGACGAG 
GTCAACGATG TCCATGGCCA TGGAGCCATA GAGCCACCGA AGACCGTGGA 
GTCAAGGCGG ACCACGTGGC TTCGCGGTAG GAGGCGTCTC GGCGGTGCGG 
TGGTCCTCGA GGGCGTAGGT GGTGACGGCG ATGGTGAAGA AGGTGCGCCA 
CTTGGGGAAG ACGTTGGTCT TGAAGTCGAG GACGATCGGG ACGAGCGAGC 
GAACGCTCTG GATGGCGGTG TCCTGC

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wmc393(10h11) Plate reference : 10h11
WMC locus number : WMC393 Length of sequence : 317
Last updated : Thu, Sep 10, 1998 at 2:03 pm
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end, 3' end not located. Bases 78 to 113 (complement) show homology to 7h5 bases 213 to 248 (36 in longest run, maxScore = 94). (CA)23 31 to 76, (CT)8 139 to 154. Too difficult to make primer.
PCR Comments : 61C anneal, complex product.
Forward primer details :
cccccTAATcTAggAccccc at 10 length 20 bases, Tm 64
Reverse primer details :
gcTccgccTAgAgcATgTgT at 186 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 196 bases.



Length of sequence : 317  Consensus sequence is :

..

GTCCGGAnnC CCCCTAATCT AGGACCCCCT CACACACACA CACACACACA 
CACACACACA CACACACACA CACACAAACA CACACACACA CTAAGACTCC 
ATCTCTCTCT CTCTCACACA CACACACTAA GACTCCATCT CTCTCTCTCT 
CTCTCACACA CACACACTAA GACTCCATCT CAAACACACA TGCTCTAGGC 
GGAGCATTTG TTCCAACCAC CCTTCACTCA ACCTTACCCA ACTGATAAAC 
AATCACCCTA ATTTACTTGT ATAACATGGA CAAGTTCCAC TTTACTTTAG 
TAGTCAGAAA CTTATCA

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wmc(10a12) Plate reference : 10a12
WMC locus number : WMC Length of sequence : 226
Last updated : Jeu 27 Aož 1998 at 12:06
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. Bases 96 to 149 (complement) show homology to 8h9 bases 43 to 96 (54 in longest run, maxScore = 72). No substantial microsatellite.
PCR Comments :



Length of sequence : 226  Consensus sequence is :

..

CGAGATAGAG AGCATGTGTG GGCCTTGAGG TGGGCAGGGG CnGGGTGAGG 
GTGTCAAAAT GTGCsTGTTG ATGCATGTGT TACATGCGAT CATGTGAGGG 
ACGGACGAAT CGAGAkrGwT G.gtTGT.Tg tGTTACGg.. ..aTGCTc.. 
...CTCTCTC TCTCA
(break)
CACACACACA AGTAAAATAG AAGACCGTTC TCTCATGTAT .caCmACGTA 
.TCgGCA.TC T

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wmc(10b12) Plate reference : 10b12
WMC locus number : WMC Length of sequence : 211
Last updated : Lun 5 juil 1999 at 17:22
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite. Bases 140 to 155 show homology to 2c3 bases 140 to 155 (16 in longest run, maxScore = 33). Bases 82 to 121 show homology to 10c2 bases 56 to 95 (40 in longest run, maxScore = 44).
PCR Comments :



Length of sequence : 211  Consensus sequence is :

..

GTACCAGGCC ATGGCGGCAT CCAAGGGAGA AGAAGCGGTC GACCAAATCT 
TTGTGTTGCA AGGAGAAGCG CTCGGGCGAG ACACGATCCG GGGCCTTGAG 
GGAAGGCATG AAGGAGAGCC CGTCGTCTAC TTGGAAGAAA CCGAGGAGGG 
GAGGGTTGCG GCGGTGGCGG CGGCGGAACC TGCGGCAGAA GCCTGGATCG 
GAGACGAGGC C

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wmc(10c12) Plate reference : 10c12
WMC locus number : WMC Length of sequence : 241
Last updated : Ven 28 Aož 1998 at 11:54
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end, 3' end not located. Bases 120 to 163 show homology to 3A7 bases 44 to 87 (44 in longest run, maxScore = 39). (CA)10 36 to 55, (CA)24 75 to 122. Too difficult to make primer.
PCR Comments :



Length of sequence : 241  Consensus sequence is :

..

GTACATCAGA CACACATTCA TCTAAAAATG TATATCACAC ACACACACAC 
ACACATTCAT CTAAAAATGT ATATCACACA CACACACACA CACACACACA 
CACACACACA CACACACACA CACTACCCGA CACCGAAGGA GTAGGGTGGC 
ACCTCGATCT TGATG.TAAT CTATCTACTC CTCTTTCAAA CACACACACA 
TACACACACA CTCGCCAGTT GTGCCTCTGT GCCTACCGCG C

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wmc394(10d12) Plate reference : 10d12
WMC locus number : WMC394 Length of sequence : 400
Last updated : Ven 28 Aož 1998 at 12:00
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : 5' end not located, trimmed to insert at 3' end. Bases 359 to 372 (complement) show homology to 14e5 bases 80 to 93 (14 in longest run, maxScore = 28). (CA)8 314 to 329, (CA)6 331 to 342, (TA)4 343 to 350.
PCR Comments : 51C anneal, product.
Forward primer details :
TTTcAgTTcAATTAcTAggcAA at 239 length 22 bases, Tm 56
Reverse primer details :
AAgAATAATTATTccTcAcccA at 372 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 155 bases.



Length of sequence : 400  Consensus sequence is :

..

GACTAACAGA TGCAGCAGAG GCAGCAAGTC AATGGTGTCA CTGAACTTGT 
GAAAAGGATG GATGAGCTGT TGTTGCTGTG TAAAATCATA GTGTTTGTGT 
TCGTGCCGAT GTTAGTAGTG ATGGTTGTAT GTGCAATGAA ATGATATCAC 
ACACTTGGTC AGTAGTGTTT CACGGTCAAG TATGTAATGA AATGATGTAG 
CAAAGATGGT GTAGCACATT GCCATGTTTG TATTTCAATT TCAGTTCAAT 
TACTAGGCAA TGATCTAGCA CACTGGACAA ATGACAAAAT AAATAGAAGA 
TGTTGCAACT TAACACACAC ACACACACAA CACACACACA CATATATATA 
GGGTCGCGCT ATTCGTCACC CTGGGTGAGG AATAATTATT CTTCATCCCC

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wmc(10e12) Plate reference : 10e12
WMC locus number : WMC Length of sequence : 259
Last updated : Lun 5 juil 1999 at 17:23
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end, 3' end not located. No substantial microsatellite. Bases 155 to 164 show homology to 26h12 bases 154 to 163 (10 in longest run, maxScore = 26).
PCR Comments :



Length of sequence : 259  Consensus sequence is :

..

GCCGCCGCCA CCGTAGTCGC GCCACCGTTG CGTGATCCGC TGCCACCAtC 
CGCTGCCCTT CTGCTGGTTG CGGTAGCCGC CGCCGCCCTT CCCTTGACGA 
CCACGGGAGG CGGAGTTTTC AGAAGACTTG AACGCGCCAG AGCCTGTGCC 
GTGAAACATC TCCATCCTCT GGTCATAGTT AGACACAAGA GAGAAGAGTT 
GATCAACAGT AATTGGGACG GGGGAGACAT CCAGCATGGA GACGATAGCC 
TGGTTAGTC

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wmc395(10f12) Plate reference : 10f12
WMC locus number : WMC395 Length of sequence : 399
Last updated : Lun 30 oct 2000 at 16:59
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Ns in sequence may be Ts. Trimmed to Rsa1 at 5' end, 3' end not located. Bases 114 to 159 show homology to 7d11 bases 96 to 141 (46 in longest run, maxScore = 67). (CT)7 87 to 100, (CT)9 112 to 129. Bases 106 to 161 show homology to TW7c07.1 bases 24 to 79 (56 in longest run, maxScore = 112).
PCR Comments : 61C anneal, smear.
Forward primer details :
TccAcAcAcATAgAcgcAAAgA at 21 length 22 bases, Tm 63
Reverse primer details :
AcTgccAggAcTgTTgATgAAT at 176 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 177 bases.



Length of sequence : 399  Consensus sequence is :

..

GTACATGTCT CTCTCTATCC TCCACACACA TAGACGCAAA GAATCTGCTA 
TCATTGmCTC AGNCTCTAGA CATATCANCA CACGCACTCT CTCTCTCTCT 
CCCTcCTCT. .CTCTCTCTC TCTCTCTCTC GCAAACACGC TCAACAAGGG 
TTTCCCCGTG AATAACTTAC CGTCTATTCA TCAACAGTCC TGGCAGTACG 
GTGAACACGA GACATGAAAA AGAATAAATC TACACGTGCT TAGACCACCT 
AGTATGACTA CTAGGACTAG AGCAAGCCAA AAAGCGCGCC GTCGCCACCC 
CCTCCTTATC GGGCAACGGG GCAAACCTTT kGTTTTAACA CAGGTCGAGG 
AAGGTTCATT GGTGGCTAAG GGCCTTCAAC AATGGCCGGA GGGCGbTTk

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wmc396(10g12) Plate reference : 10g12
WMC locus number : WMC396 Length of sequence : 254
Last updated : Ven 28 Aož 1998 at 12:37
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end, 3' end not located. (GT)14 122 to 149.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
TgcAcTgTTTTAccTTcAcggA at 27 length 22 bases, Tm 65
Reverse primer details :
cAAAgcAAgAAccAgAgccAcT at 151 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 146 bases.



Length of sequence : 254  Consensus sequence is :

..

GTACAATCGG GGTTTTTGAA CCATGTTGCA CTGTTTTACC TTCACGGATT 
CATAAGCAAT ATATTCTTTT cGTTCTTGTG TGCAATCCTC CCATCTCTGC 
TGGGTCTCTT GTGTGCTTCT CGTGTGTGTG TGTGTGTGTG TGTGTGTGTA 
AGTGGCTCTG GTTCTTGCTT TGCTCCTCGT GCCAAGtCCA AGAAGAGAtC 
TCATAGATGC AAGCGCCATG TTGAGTCTGA AGAGGAACAA nAGGTTGTGG 
TAGT

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wmc397(10h12) Plate reference : 10h12
WMC locus number : WMC397 Length of sequence : 209
Last updated : Ven 28 Aož 1998 at 12:44
Owner : Jacquemin, Gembloux Date at Agrogene : 18.6.98 Public Domain : Yes
Comments : 5' end not located, trimmed to Rsa1 at 3' end. (CA)9 69 to 86, (GA)14 93 to 120.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
AgTcgTgcAccTccATTTTg at 46 length 20 bases, Tm 63
Reverse primer details :
cATTggAcATcggAgAccTg at 186 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 160 bases.



Length of sequence : 209  Consensus sequence is :

..

ATGACTACCA GGTGTTGTCG ACTGTGGrwT TGCTGCAACC AACTCGAGTC 
GTGCACCTCC ATTTTGGACA CACACACACA CACACATACA CAGAGAGAGA 
GAGAGAGAGA GAGAGAGAGA GGCAATGACT TGGGCGATCA TGGGGCCGAT 
CGCGAGAGAT CGGAGCGGCA CgCCAGGAGG GAGCTCAGGT CTCCGATGTC 
CAATGGTAC

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wmc(22a7) Plate reference : 22a7
WMC locus number : WMC Length of sequence : 348
Last updated : Mar 30 mai 2000 at 9:54
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 193 to 234 show homology to 8g4 bases 177 to 218 (42 in longest run, maxScore = 58). (CT)43 195 to 280. Duplicate with 8g4, 8g4 has precedence. Bases 187 to 234 show homology to TW3d02.1 bases 83 to 130 (complement) (48 in longest run, maxScore = 33). Bases 140 to 280 show homology to TW4e06 bases 76 to 216 (complement) (141 in longest run, maxScore = 149). 22a7 is a duplicate of TW4e06, 22a7 has priority.
PCR Comments :
Expected size of amplified fragment (Chinese Spring=) bases.



Length of sequence : 348  Consensus sequence is :

..

AGGAAAGAAC AACACAAGAG ACATAGGGTT TATACTGGTT CGGGCCACCG 
TTGTGGTGTA ATACCCTACT CCAGTGTGTG GTGTGTGGAT TGCCTCTTGG 
GCTGATGATG ATGAACAATA CAAGGAAGAA CAGCCTCGTG AGGGTCTGTT 
CTTGGCTGAG GCGATGAACT GCTGGGAGGA GTTCAGCCAC CTTTCTCTCT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT GACCGACCCC CTTCCTGTGT 
GGGTGGCTGG TCCTATTTAT AGAGGCCCTG GTCCTCTTCC CAAATATT

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wmc(22b7) Plate reference : 22b7
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98
PCR Comments :
No sequence available for this clone
Go back to index

wmc398(22c7) Plate reference : 22c7
WMC locus number : WMC398 Length of sequence : 153
Last updated : Mar 17 Nov 1998 at 14:05
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end, corrupted 3' end removed as cleanly as possible. Bases 149 to 198 show homology to 11a8 bases 348 to 397 (50 in longest run, maxScore = 42). (GT)14 32 to 59. Note that the 3' primer is located close to corrupted sequence of vector.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
ggAgATTgAccgAgTggAT at 1 length 19 bases, Tm 60
Reverse primer details :
cgTgAgAgcggTTcTTTg at 136 length 18 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 152 bases.



Length of sequence : 153  Consensus sequence is :

..

GGAGATTGAC CGAGTGGATA TATGCACTCC CGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTA GTGATATCTA TATCTACTCG TGCATTAGTG TACGATGAAA 
AGATATGATA AGAATTGATT AACCTCCCCC TTGACCAAAG AACCGCTCTC 
ACG

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wmc(22d7) Plate reference : 22d7
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(22e7) Plate reference : 22e7
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(22f7) Plate reference : 22f7
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(22g7) Plate reference : 22g7
WMC locus number : WMC Length of sequence : 267
Last updated : Mar 22 DŽc 1998 at 17:34
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 182 to 224 show homology to 13g1.2 bases 346 to 388 (43 in longest run, maxScore = 82). (GT)8 23 to 38, (GT)14 89 to 116, (GT)9 139 to 156. Too difficult to make primer. Bases 15 to 45 show homology to 2f3 bases 268 to 298 (31 in longest run, maxScore = 49). True duplicate with 2f3, 22g7 has priority.
PCR Comments :



Length of sequence : 267  Consensus sequence is :

..

GTGGGTAGGT GGGTGTGCGT GCGTGTGTGT GTGTGTGTTT GTGTGAGAGG 
GAGAGAGAGA GAGATGGAGT CTTAGTGTGT GTAGGTGGGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGCAT GCGTGCGTGT GTGTGTTTGT GTGTGTGTGT 
GTGTGTCGCG GGGTCCTTGG TGGCGGATGT CATTTAAGTG TGCATGGCTT 
CATCATAGAG AGGTGATGTG TATGTCAGAG GCCACCAACG ATGGGGTGCG 
AGAGAGAAAA TGCCCGT

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wmc(22h7) Plate reference : 22h7
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc399(22a8) Plate reference : 22a8
WMC locus number : WMC399 Length of sequence : 316
Last updated : Lun 2 Nov 1998 at 16:54
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)13 260 to 285.
PCR Comments : 51C anneal, product.
Forward primer details :
cTTcAgAgATgTTTgATTAccT at 137 length 22 bases, Tm 55
Reverse primer details :
ggTATTgcTAAcTgAATgATgT at 287 length 22 bases, Tm 55
Expected size of amplified fragment (Chinese Spring=) 172 bases.



Length of sequence : 316  Consensus sequence is :

..

GCTCGGCTTC GAGCTGAGAC GGCTCGCGAG CTTCTGGCCG AGAGACTTGT 
CAGCCTGCAC GATCCCACGA GAAGCAATTC AGTATGATAT CTTACTATAA 
TCTTCAGAGA ACATGGAAGA AATTACACAC AAATTTCTTC AGAGATGTTT 
GATTACCTGA GACCAGTAGG AGAGCCAGAT GGCCTTGATC TCGTGGGTGA 
GGCGGGGGTC GGACAGCGCA TCGATCCATC TGTTGATGAA TCGCTCTTGC 
CTGAACATAC ACACACACAC ACACACACAC ACACATACAT CATTCAGTTA 
GCAATACCAA ATTGAT

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wmc(22b8) Plate reference : 22b8
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(22c8) Plate reference : 22c8
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(22d8) Plate reference : 22d8
WMC locus number : WMC Length of sequence : 155
Last updated : Lun 28 DŽc 1998 at 16:14
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 40 to 116 (complement) show homology to 4f9 bases 224 to 300 (77 in longest run, maxScore = 68). (GT)13 17 to 42. Duplicate, 4f9 has precedence. Bases 1 to 155 show homology to 20c3 bases 1 to 155 (155 in longest run, maxScore = 107). Duplicate with 20c3, 22d8 has priority.
PCR Comments :



Length of sequence : 155  Consensus sequence is :

..

CCTTCGGTGC CGGGTAGTGT GTGTGTGTGT GTGTGTGTGT GTTTGAGAGA 
GAAGCCAGTC GATAGATTAG TATCAAGATC GAGGTGTCAC CCTACTCCTT 
CGGTGTTGGG AAGTGTTGGT GTGGGGGGGG GGCAGTGACA CTCACATATC 
TCTAC

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wmc(22e8) Plate reference : 22e8
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(22f8) Plate reference : 22f8
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
Go back to index