Wheat microsatellite WMC 25/06/01

wmc(22g8) Plate reference : 22g8
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22h8) Plate reference : 22h8
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc400(22a9) Plate reference : 22a9
WMC locus number : WMC400 Length of sequence : 146
Last updated : Lun 2 Nov 1998 at 16:12
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to insert at 3' end, 5' end not located. Bases 121 to 146 (complement) show homology to 5e3 bases 168 to 193 (26 in longest run, maxScore = 25). (CA)21 56 to 97, (CT)9 26 to 43,
PCR Comments : 51C anneal, complex product.
Forward primer details :
TgTTcgATgTTgTgcTATgTcTgT at 2 length 24 bases, Tm 64
Reverse primer details :
cTTggATggggATgAATTgTg at 124 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 143 bases.



Length of sequence : 146  Consensus sequence is :

..

GTGTTCGATG TTGTGCTATG TCTGTCTCTC TCTCTCTCTC TCTCCCTCTC 
TCTCTCACAC ACACACACAC ACACACACAC ACACACACAC ACACACATAC 
ACACACACAC ACTCCCCCAG GCACACAATT CATCCCCATC CAAGGT

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wmc(22b9) Plate reference : 22b9
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22c9) Plate reference : 22c9
WMC locus number : WMC Length of sequence : 160
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 160  Consensus sequence is :

..

GTCCATACCC AAAATGATGT TTATGTCCGA GGACTTGAGA GCTATTAAGG 
ATGCAAGGAA GACAAGTCTA TCGACAAGAA TTTCGTTCCC ATGGCTTACT 
CTAGAAGTCG TCGTCGTCGC CGCCTCCGCC GCCGCCGTCC CTACTGCATC 
CCTCCCCCGG

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wmc401(22d9) Plate reference : 22d9
WMC locus number : WMC401 Length of sequence : 176
Last updated : Lun 5 juil 1999 at 17:22
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 10 to 52 show homology to 19c3 bases 7 to 49 (43 in longest run, maxScore = 54). (GT)10 119 to 138. Bases 22 to 52 show homology to 26g7 bases 172 to 202 (complement) (31 in longest run, maxScore = 58).
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
tcgtcacctcttctcttcttgt at 6 length 22 bases, Tm 60
Reverse primer details :
CCTCTAGGGCATATTTCCTTCA at 154 length 22 bases, Tm
Expected size of amplified fragment (Chinese Spring=) 170 bases.



Length of sequence : 176  Consensus sequence is :

..

AATATTCGTC ACCTCTTCTC TTCTTGTACG TACTCCATTT GTATCTCACT 
TCATGTGGCT TCACACTCAC ACGATATTCC TATTCTATGA ACCTGTGTGT 
GTGTGTGTGC GCGCGTGCGT GTGTGTGTGT GTGTGTGTWT AACATCATGT 
ATATGAAGGA AATATGCCCT AGAGGC

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wmc(22e9) Plate reference : 22e9
WMC locus number : WMC Length of sequence : 244
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 244  Consensus sequence is :

..

GCTCCTCTTG CTAGTTGCTG CTGCCCTGCT ATAGCTGCTG CTGCTACTGA 
TGGGCACGAC CACGGCCATG GCCGTGGGTG TGTGTTGTGT GTGCGTGTAG 
TGTGTGCGTG TGCGTGTGCG TGTGTAATTA GCTTAGGGAC TAACTACTAG 
TTAGTTTAAC TACTAGCCTA TGACAAGTGG ACCCCTAGAC TAATTAATCT 
AATTAACTAG AATGTTTTAG TTGCTGCCTA TGACATGTGG GCCA

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wmc(22f9) Plate reference : 22f9
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc402(22g9) Plate reference : 22g9
WMC locus number : WMC402 Length of sequence : 209
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 109 to 142 show homology to 11f8 bases 401 to 434 (34 in longest run, maxScore = 52). (CA)15 66 to 95. Bases 120 to 138 show homology to 2h2 bases 155 to 173 (complement) (19 in longest run, maxScore = 38).
PCR Comments : 61C anneal, product.
Forward primer details :
gcccAAAATgTcAcggATgT at 16 length 20 bases, Tm 65
Reverse primer details :
TAgcTTggTTTggAgcgAgg at 135 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 139 bases.



Length of sequence : 209  Consensus sequence is :

..

AGAAAAAATC GTTCGGCCCA AAATGTCACG GATGTGTCTT TCTTTTGTAG 
TGCTTGTACC CCCTCCACAC ACACACACAC ACACACACAC ACACACCCCA 
AATCCCCTGC TCACACACAT ACACACCACC CCTCCCTCGC TCCAAACCAA 
GCTAGGTCGC CATCGCCGCC GCCGCGCCGC CTCCATCTTC AACCTCTGCC 
CATCTGCCC

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wmc403(22h9) Plate reference : 22h9
WMC locus number : WMC403 Length of sequence : 198
Last updated : Mar 3 Nov 1998 at 11:01
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end, 3' end not located. Bases 71 to 131 show homology to 11h2 bases 225 to 285 (61 in longest run, maxScore = 92) - to one side of microsatellite. (GT)12 139 to 162. Reverse primer has no apparent homology with 11h2
PCR Comments : tested 51C anneal, no product.
Forward primer details :
ggcAAAgAAgcAcAAgcgAg at 70 length 20 bases, Tm 65
Reverse primer details :
gcgTAAgcAAgAgcAcAgcA at 165 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 115 bases.



Length of sequence : 198  Consensus sequence is :

..

GCGGTGTGGT CTCCGTCTTT GATCTTCTAG TTGCCCTCTT TGAAGATTGA 
GGTGTTGTGC ATGTTGGGGG GCAAAGAAGC ACAAGCGAGA GTGGTCGCCG 
TATAACCTTG GATGGGGATG AATTGTGTGC TTGGGGGAGT GTGTGTGTGT 
GTGTGTGTGT GTTGTGCTGT GCTCTTGCTT ACGCGCCTCG AGGGATCC

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wmc(22a10) Plate reference : 22a10
WMC locus number : WMC Length of sequence : 214
Last updated : Lun 2 Nov 1998 at 9:55
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to innsert at 5' end, 3' end not located. Bases 167 to 214 show homology to 11g5 bases 688 to 735 (48 in longest run, maxScore = 41). Bases 177 to 214 show homology to 10c1 bases 114 to 151 (38 in longest run, maxScore = 35). No substantial microsatellite.
PCR Comments :



Length of sequence : 214  Consensus sequence is :

..

CCGACAGTTG TACACAATAT CCGGGTCAAC TTGTTATTCT CCGCCCCGCT 
GGGCTGCAAA CTTTCCTAGG AGGTATGCAT TAGGCTTAAC AATAAAATGG 
ACTTTAAGAA ATAATAAATG GGATGTAATT ATAATAACTG GGCAGTAACT 
ATAAAAAATG CACCAAACGC GCCTCGAGGG ATCCTCTAGA GTCGACCTGC 
AGGCATGCAA GCTT

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wmc(22b10) Plate reference : 22b10
WMC locus number : WMC Length of sequence : 556
Last updated : Lun 30 oct 2000 at 16:59
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 9 to 55 (complement) show homology to 7b4 bases 276 to 322 (47 in longest run, maxScore = 62). (GA)11 57 to 78, (GA)9 153 to 170, (GA)10 256 to 275, (GA)23 302 to 347, (GA)8 356 to 371, (GA)46 374 to 465. Too difficult to make primer. Bases 170 to 211 show homology to 21e7 bases 59 to 100 (42 in longest run, maxScore = 53). Bases 153 to 244 show homology to TW5b02 bases 108 to 199 (92 in longest run, maxScore = 63). Bases 159 to 230 show homology to TW7h07 bases 80 to 151 (72 in longest run, maxScore = 61).
PCR Comments :



Length of sequence : 556  Consensus sequence is :

..

AGGGACCAAC AAGAATTTTG TGTTGGATGC GTGCGACAGA ATTGAAGATT 
GTCGGTGAGA GAGAGAGAGA GAGAGAGAGG GAGAGAGAGA GAGGGAGAGA 
GAGGGAGAGA GAGAGAGATG GAGATAAAGA GAGAGGGAGA GAGAGAGAGA 
GTGAGAGAGA GAGAGAGAGA TGGAGAGAAA GAGATGGAGA GAAAGGGAGA 
GGGACCAACA AGAATTTTGT GTTGGATGCG TGCGACAGAA TTGAGGATTG 
TCAGTGAGAG AGAGAGAGAG AGAGAGGGAG AGGGAGAGAG AGGGGGAGAG 
GGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGA
(break)
AGAGAGAGAG AGAGAGAGGG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG 
AGAGAGAGAG AGGAGGGGGA GGGGGAGGGA GAGGGAGAGA GAGAGTCACG 
TTTTAAGCAT CAAAGACTGA TATAAACCAT GTTTCGATAT AAACTTGCAT 
TGAAATATT

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wmc404(22c10) Plate reference : 22c10
WMC locus number : WMC404 Length of sequence : 286
Last updated : Mar 3 Nov 1998 at 13:13
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CT)4 34 to 41, (CA)25 56 to 105.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
AcgAgTTggTTgTgTcgccT at 8 length 20 bases, Tm 65
Reverse primer details :
AgcATATgcATTTcgTTTggg at 160 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 173 bases.



Length of sequence : 286  Consensus sequence is :

..

TTTCATCACG AGTTGGTTGT GTCGCCTCCC CATCTCTCTC TTTCTCTCGC 
TCTAACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC 
ACACATGTGC ACGCGCACAC AACAAAAAAT GTCATCTCCA CTGTACATCA 
TGTACTATAC CCAAACGAAA TGCATATGCT AATTAATGAC TGATCGCTCT 
ATGCACTTAA GGATTCTCAC GAGAAACGCT TAACAACGAA TGTGATGTTT 
ACGCATGTCA AAGAGGCATA CTGTCATGCC CGCCTG

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wmc(22d10) Plate reference : 22d10
WMC locus number : WMC Length of sequence : 266
Last updated : Lun 2 Nov 1998 at 9:54
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 23 to 56 show homology to 11h6 bases 171 to 204 (34 in longest run, maxScore = 43). No substantial microsatellite.
PCR Comments :



Length of sequence : 266  Consensus sequence is :

..

AATATTGGAC AGTGGGTATT GAAAGTCATG ACCGCGGGCA CAGCGGACAA 
GGTGGCTTTG TATTGGTATG CTGAGCCGTC TCTTTTAGTA ACTACACATG 
AGCTAGTGTG GTGATTATAC ACAAACGCAT GCATGCACAC GAAATGCATG 
GATGCAACAC TTGCACGTAC ACACACAGCC ACGCAACACT CACACGCACA 
CATGCAACAC TCACACGCAC ACACACGCAT GCATGCACAC ACCACACGAC 
CATCACACAC GCTCAC

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wmc(22e10) Plate reference : 22e10
WMC locus number : WMC Length of sequence : 252
Last updated : Mer 14 juin 2000 at 10:35
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 28 (complement) show homology to 3d2 bases 188 to 215 (28 in longest run, maxScore = 49). (CT)9 63 to 80, (CT)13 152 to 177. Too difficult to make primer. Bases 1 to 252 show homology to 25a12 bases 1 to 252 (252 in longest run, maxScore = 147). Duplicate with 25a12, 22e10 has priority. Bases 84 to 110 show homology to TW1f12 bases 26 to 52 (complement) (27 in longest run, maxScore = 33).
PCR Comments :



Length of sequence : 252  Consensus sequence is :

..

CACACAAACC TTGTTGGTCC CTCTCCCTTT CTCTCCATCT CTCTCTCTCT 
CTGTCTCTCT GTCTCTCTCT CTCTCTCTCT CCCACAATCT TCAATTCTAT 
CGCACGCATC TAACACACAA ACCTTGTTGG TCCCTCTTCC CTTCTCTCCA 
TCTCTCTCTC TCTCTCTCTC TCTCTCTCAC CCACAATCTT CAATTCTGTC 
GCACGCATCT AACACACAAA CCTTGTTGAT CCCTCTCCTT TCTCTCCATC 
TC

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wmc(22f10) Plate reference : 22f10
WMC locus number : WMC Length of sequence : 501
Last updated : Mar 3 Nov 1998 at 13:22
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 396 to 463 show homology to 19d1 bases 148 to 215 (68 in longest run, maxScore = 119). (CT)14 275 to 302. True duplicate with 19d1, 19d1 has precedence.
PCR Comments :



Length of sequence : 501  Consensus sequence is :

..

AATATTTTGT GTGGTGAAGA TGGAGTATAG CCAGACCATA TGATTTCTTT 
TTCACCGCCA CACACACAGT CTCCACTCGT CTTTCACTTT GTCTTTCTCT 
CTATGGGATT CTCTCACACA CCCTATATGT CTCTACATAT GACTCACACC 
ACTAACATTA CTCTATCTCT CTCTCTCCCG TAGACACAAC ATTTGTTGCG 
GTCTCCTTTT CGTCTCCATC CCAGACTGAC ATTATTCATT TGTTTACCGA 
TCAGACAACC CCGACTACTG TCTCCTCTCT CTCTCTCTCT CTCTCTCTCT 
CTCAGACACA CACACACAAC TCCTGCTTCA ATTCCCCTTC CCGACTACCG 
TCTCTCTCTC ACACACACAA AACTCTCGCT TTCGCACCCC GACTTGTATT 
TCTCTCACAA ACATTTATCG ACTAGCTAGC CTCTAGATAG GTTTATACAC 
CCGCACACTC GTGTTTCCAC CATCGCATAC ATGACTCTCT CCCGCTCCTT 
G

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wmc(22g10) Plate reference : 22g10
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22h10) Plate reference : 22h10
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22a11) Plate reference : 22a11
WMC locus number : WMC Length of sequence : 325
Last updated : Lun 20 sep 1999 at 16:57
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Forward sequence only. Trimmed to insert at both ends. Bases 8 to 325 show homology to 22a12 bases 9 to 326 (318 in longest run, maxScore = 256), 22a11 has priority. Bases 153 to 167 show homology to 24c7 bases 66 to 80 (15 in longest run, maxScore = 46). (GT)8 301 to 316. No room for primer. Bases 228 to 245 show homology to 10a5.1 bases 57 to 74 (18 in longest run, maxScore = 29).
PCR Comments :



Length of sequence : 325  Consensus sequence is :

..

GGTTATACAA ATCCTTGCTC TATTGATCAT ATCGCCTTGC CCTGAAAAGC 
AAGATTGTTC TCGAGCTTCG TAACATACCG GTGGTTCGTG ATTTTCTGAA 
TATCTTCTCA GAAGTATTAC CAGGTTGTCA CCTGACCGCT ATGTTGAGTT 
CGTGATCAAG TTGGTTTATT CCTGTAAACC ACCCCTTCTC CAAGAATCCG 
TGTTGGATAC CCTGAGCTAG TTGGATAAGC TAAACAACAA CTTGGAGAGC 
TGGAAGATAA AAGCTTGTCC GACTTAGTTC ATGTTAAGGG ATATCTTTTT 
GTGTGTGTGT GTGTGTGCGT GTGTG

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wmc(22b11) Plate reference : 22b11
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22c11) Plate reference : 22c11
WMC locus number : WMC Length of sequence : 410
Last updated : Lun 2 Nov 1998 at 9:54
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 190 to 199 show homology to 5c12 bases 134 to 143 (10 in longest run, maxScore = 21). Numerous small repeats (CA), no room for primer.
PCR Comments :



Length of sequence : 410  Consensus sequence is :

..

GGCCACACAC ACTCACGCAC ACACACACAT CTCACGGCCA CACACACATA 
ACACACACTC ACGCACGCAC ACGTAGCACA CACACGCACA TCTCACGGCC 
ACACACACAG AACACACACT CACGCACACA CACATAGCAC ACACATGCAC 
ATCTCACGGC CACACACACA GCGCCATGGC CAGCTCCAAC AACAGCAGCC 
GGCGGCAGTA GCAGCCATGG GCGCGAGGCA AGCAGCAATG CAGTAGGCCG 
CAGCATGCAG GAGTGCACGC AGTGGCAAGC AGAGCAGCCC CGCTGCAAGT 
AACAGAACAA GGCAGCGACA GCAACTAAGA CAGAGCAGCG GCAGCGGTGG 
CCGGAAGCAG CAGCACGGCA ACTGTAACGC CCCGTGACCG ATGTGCCAGG 
TGTCGTCTAG

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wmc405(22d11) Plate reference : 22d11
WMC locus number : WMC405 Length of sequence : 289
Last updated : Mer 4 Nov 1998 at 9:25
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 118 to 133 show homology to 2a9 bases 852 to 867 (16 in longest run, maxScore = 20). (CA)21 133 to 174, (CT)9 115 to 132.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Forward primer details :
gTgcggAAAgAgAcgAggTT at 41 length 20 bases, Tm 63
Reverse primer details :
TATgTccAcgTTggcAgAgg at 239 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 218 bases.



Length of sequence : 289  Consensus sequence is :

..

GCTCACCAGA TCTGCCAACG CTGAGATCGG TTGTGCTGGG GTGCGGAAAG 
AGACGAGGTT ACGTGTGTGA GGAGAAGACG AGGGACTAGC CAATTGGGAA 
GGACAAAACA CGTGCTCTCT CTCTCTCTCT CTCACACACA CACACACACA 
CACACACACA CACACACACA CACAAAGACA CAATGTTCAC TACACACACA 
ATGTACCTCT CTCGCCATTC GCTTCACCAC AAAATGCGCC TCTGCCAACG 
TGGACATAGT GAGAGGGTGC CCTTGCATTC GTCAATATT

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wmc(22e11) Plate reference : 22e11
WMC locus number : WMC Length of sequence : 148
Last updated : Mer 4 Nov 1998 at 9:26
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 44 to 80 show homology to 5b9 bases 170 to 206 (37 in longest run, maxScore = 74). (GT)11 42 to 63. True duplicate, 5b9 has precedence.
PCR Comments :



Length of sequence : 148  Consensus sequence is :

..

ATCAAGATCG AGGTGCCACC CTACTCCTTC GGTGTCGGGT AGTGTGTGTG 
TGTGTGTGTG TGTTTGAGAG AGAAGCCAGT TGATAGATTA GTATCAAGAT 
CAAGGTGTCG CCCTACTCCT TCAGTGTCGG GAAGTGTGTG TGTGAGTG

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wmc(22f11) Plate reference : 22f11
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22g11) Plate reference : 22g11
WMC locus number : WMC Length of sequence : 534
Last updated : Lun 4 Jan 1999 at 12:03
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 340 to 372 show homology to 12a9 bases 372 to 404 (33 in longest run, maxScore = 131). Bases 112 to 136 show homology to 21f5 bases 117 to 141 (complement) (25 in longest run, maxScore = 33). (CA)9 174 to 191, (CA)12 194 to 217. 22g11 is very similar to 12a9 but it's not a duplicate. In case of primers of 12a9 doesn't worked, design primers with 22g11. Bases 185 to 217 show homology to 20g10 bases 37 to 69 (complement) (33 in longest run, maxScore = 44). Bases 185 to 217 show homology to 20g10 bases 37 to 69 (complement) (33 in longest run, maxScore = 44).
PCR Comments :



Length of sequence : 534  Consensus sequence is :

..

TCATCAACGC GCATATTTTG GCACCCTCAC CCGGCCCCTG CCCATCTCAA 
GGCCCGCACA TCGCTCTCTA TCTCGCACGC ACAATCTCTT CGTGAATACC 
CTATTTAGCA TGTAGCTTTC CGTCACATAC ACACACGTTT CTCTCCTTAG 
GTTTGTATTC TCTCGATATC TGACACACAC ACACACACAC ACGCACACAC 
ACACACACAC ACACACACCC ACACACCCAC ACACCCACAC ACACCCTCCC 
CTGAGCACAC AATTCATCCC CATCCAAGGT TATATGGCGA CCACTCTCGC 
TTGTGCTTCT CTACACCCCA GCATGCACAA CACCTCAATC TTCAAAGAGG 
GCATCGAGGA GATCGAAGAC GGCGACCACA CTGTGCTAGA GAATGCGGGG 
CCATTTGGAA GCTGGATGAT GTGACGACGG CTGACTGACT CCTCCCCCCA 
CCATCTCTCT CTCAGACACA CAAAATTACC AATCCCTCTT TCCCCACACA 
AAATTATCAA TCCCTCAACC CACGAGATCC TTCC

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wmc(22h11) Plate reference : 22h11
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22a12) Plate reference : 22a12
WMC locus number : WMC Length of sequence : 326
Last updated : Mer 4 Nov 1998 at 9:59
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 9 to 326 show homology to 22a11 bases 8 to 325 (318 in longest run, maxScore = 256). (GT)8 302 to 317. Duplicate, 22a11 has precedence.
PCR Comments :



Length of sequence : 326  Consensus sequence is :

..

GGTTATTTCA AATCCTTGCT CTATTGATCA TATCGCCTTG CCCTGAAAAG 
CAAGATTGTT CTCGAGCTTC GTAACATACC GGTGGTTCGT GATTTTCTGA 
ATATCTTCTC AGAAGTATTA CCAGGTTGTC ACCTGACCGC TATGTTGAGT 
TCGTGATCAA GTTGGTTTAT TCCTGTAAAC CACCCCTTCT CCAAGAATCC 
GTGTTGGATA CCCTGAGCTA GTTGGATAAG CTAAACAACA ACTTGGAGAG 
CTGGAAGATA AAAGCTTGTC CGACTTAGTT CATGTTAAGG GATATCTTTT 
TGTGTGTGTG TGTGTGTGCG TGTGTG

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wmc406(22b12) Plate reference : 22b12
WMC locus number : WMC406 Length of sequence : 373
Last updated : Mer 4 Nov 1998 at 10:15
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at both ends of insert. (CA)16 188 to 219.
PCR Comments : 61C anneal, polymorphic on acrylamide (though weak).
Forward primer details :
TATgAgggTcggATcAATAcAA at 116 length 22 bases, Tm 61
Reverse primer details :
cgAgTTTAcTgcAAAcAAATgg at 311 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 217 bases.



Length of sequence : 373  Consensus sequence is :

..

AATATTATTG TGTTTAAGAT ATAGGTAGGA GTTAGAGATA GAATAGGTTT 
AACATGTACG ACTTGCCCTC TACTCCAAGT CTCTCTCCCT CACAATGTAT 
TCTATATATA CCTCATATGA GGGTCGGATC AATACAACAG AAACAACACC 
ATACCCATCC ATCCTATAAT TTTTATACAC ACCACAACAC ACACACACAC 
ACACACACAC ACACACACAT ACACACACGG AGAGCAATAG CATAGCAGCA 
GCAGCAGCAA GCAGGGGACT CATGCTAAGT TAAATGTCCT CTCTTATGTA 
CAAAACTTTT CCATTTGTTT GCAGTAAACT CGACGAAACA ACATGCAAAA 
ATATCAGCCT TGAGAACAAT ATT

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wmc(22c12) Plate reference : 22c12
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22d12) Plate reference : 22d12
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22e12) Plate reference : 22e12
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : No sequence 1.9.98.
PCR Comments :
No sequence available for this clone
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wmc(22f12) Plate reference : 22f12
WMC locus number : WMC Length of sequence : 362
Last updated : Jeu 5 Nov 1998 at 15:50
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to insert at both ends (NB 5' linker corrupted). Bases 183 to 264 show homology to 5b3 bases 154 to 235 (82 in longest run, maxScore = 97). (CA)8 92 to 107, (CA)9 122 to 139, (CT)10 66 to 85. Duplicate, 5b3 has precedence.
PCR Comments :



Length of sequence : 362  Consensus sequence is :

..

ACTACTTAGA CTCCATCTCT CTCTCTCACA CACACACACA AACTAAGACT 
CCATCTCTCT CTCTCCTCTC TCTCTCTCTC TCTCTCACTC TCACACACAC 
ACACACACGC ACATACACAC GCACACACAC ACACACACAC CACACACACT 
AAGACTCCAT CTCACACACA CATGCTCTAG GCGGAGCATT TGTTCCTACC 
ACCCTTCATC CAACCTTACC CGTATGATAA ACAATCACCT TTATTTACTT 
GCATAACATG GACAATAATT TTACAAAAGA TACCCGCACC TCTCTCCACA 
TCATTATCTC CCGCAACCTC ATTGCTCAGC AATTAAAAAA GGAATAAGTC 
TCTGGAACAA TC

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wmc(22g12) Plate reference : 22g12
WMC locus number : WMC Length of sequence : 212
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 212  Consensus sequence is :

..

CCCCATGAGG GCCTCCTCCA CCCATGCCGC CGAGAGGGCC CCCGCCTCCC 
ATGCCGCCAC CAAGAGAGCC TCCTCCACCC ATGCCGCCGA GAGAGCCCCC 
GCCTCCCATG CCACCACCAA GAGAGCCTCC GCCGCCTATG CCACCAAGAG 
AGCCTCCGCC GCCCATGCCC CCAAGAGAGC CCCCCCCGCC CATGCCGCCG 
AGAGAGCCTC CT

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wmc(22h12) Plate reference : 22h12
WMC locus number : WMC Length of sequence : 229
Last updated : Jeu 5 Nov 1998 at 15:57
Owner : Romestant, RAGT Date at Agrogene : 1.9.98 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to insert at both ends. (TTG)45 38 to 172, (TTG)18 176 to 229. No room for primer.
PCR Comments :



Length of sequence : 229  Consensus sequence is :

..

GCTGTTGTTG TTGCTGTTGT TGTTGTTGCT GCTGCCGTTG TTGTTGTTGT 
TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG 
TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT 
GTTGTTGTTG TTGTTGTTGT TGTTTTTGTT GTTGTTGTTG TTGTTGTTGT 
TGTTGTTGTT GTTGTTGTTG TTGTTGTTG

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wmc(10A1.1) Plate reference : 10A1.1
WMC locus number : WMC Length of sequence : 73
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert.Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 73  Consensus sequence is :

..

GAGCGGCCGC GACGACGATC GGTGGCCGCG GTGCGTTGCA TTGGTTGGCC 
TCGCAAGGAT GATGGTGGGA CGG

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wmc(10A1.2) Plate reference : 10A1.2
WMC locus number : WMC Length of sequence : 176
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 35 to 63 (complement) show homology to 5g3 bases 405 to 433 (29 in longest run, maxScore = 42). (GT)8 18 to 33, (GT)11 36 to 57. Duplicate with 5g3, 5g3 has precedence. Bases 90 to 146 show homology to 24C10 bases 92 to 148 (57 in longest run, maxScore = 66).
PCR Comments :



Length of sequence : 176  Consensus sequence is :

..

TGTGCGTGTG CGTGTATGTG TGTGTGTGTG TGTTTGTGTG TGTGTGTGTG 
TGTGTGTTTA ACATCATGTG TGTGTTAGAG AGAGTGACGC ATCGACCTAC 
TCCTACAGAG AGATGGTGTG TAGTGTATGA TTTAGCCGCG AGAGTCGGTG 
CACCCTCTCG TTTTCGGGCG CCTGGT

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wmc(10b1) Plate reference : 10b1
WMC locus number : WMC Length of sequence : 198
Last updated : Mar 10 Nov 1998 at 14:52
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 13 to 40 show homology to 8e7 bases 280 to 307 (28 in longest run, maxScore = 35). Bases 118 to 141 show homology to 21b4 bases 36 to 59 (complement) (24 in longest run, maxScore = 75), duplicate, 10b1 has priority. No substantial microsatellite.
PCR Comments :



Length of sequence : 198  Consensus sequence is :

..

gctctggact AAGGCGCGGC GGCGGCGAGC GAAGGCAAGA TCTTGGGAAG 
GAGGAAGAAC GGGACGGCGG TTCCGAGATT GGGGAAAGCA TGAAGGGTGA 
ATGAGCAGCG TGCCCGCGGT TATTCCTTTT TACGGGGAAG GTGCCAGCCG 
CCTATTTTCC CATTAACTGC GATGTgtcgc acgcgcctcg agggatcc

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wmc(10c1) Plate reference : 10c1
WMC locus number : WMC Length of sequence : 151
Last updated : Lun 2 Nov 1998 at 9:55
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 114 to 151 show homology to 22a10 bases 177 to 214 (38 in longest run, maxScore = 35). No substantial microsatellite.
PCR Comments :



Length of sequence : 151  Consensus sequence is :

..

actACATTTC GATCTACTGT CCACATTCAT ATGACGCCTC AGTATATAGT 
GATGTTAGGG GGGAAGGGCC CTTCCAGCCC TTGTCTTCGC CATTGTGCAC 
GCGCCtCGgt ccaagggatc ctctagagtc gacctgcagg catgcaagct 
t

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wmc(10e1) Plate reference : 10e1
WMC locus number : WMC Length of sequence : 270
Last updated : Jeu 5 Nov 1998 at 16:07
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Bases 179 to 227 show homology to 5h9 bases 26 to 74 (49 in longest run, maxScore = 56). (GT)9 38 to 55, (GT)18 58 to 93. Duplicate, 5h9 has precedence.
PCR Comments :



Length of sequence : 270  Consensus sequence is :

..

ggccgtgact ACCTCCCGCC AACTGAAGTG CGCGCGCGTG TGTGTGTGTG 
TGTGTGCGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGATGGGG 
GCATGCGTTC ATAGCTTAGG ATTCCCCTCC TAGCGACACG AGGGGGGAAG 
CACTATTACC TTATCAAGTT CTACTTTTCT CCCACTCACT TCTTTCGAGA 
GAAACTCCTT CTCTAGAAAG GATCCATCTT AGCAACAATA TCTTGCCTTT 
GGATCTGTGA TAGAAGGTGT

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wmc(10f1) Plate reference : 10f1
WMC locus number : WMC Length of sequence : 356
Last updated : Jeu 5 Nov 1998 at 16:11
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. (CA)8 74 to 89, (CT)14 46 to 73. Too difficult to make good primer.
PCR Comments :



Length of sequence : 356  Consensus sequence is :

..

tctgtggact aACAACACAC ACATGCAAAT AAGTCTATAA CAAATCTCTC 
TCTCTCTCTC TCTCTCTCTC TCTCACACAC ACACACACAT GTATAGTCTA 
TAGTTTTAAT ACTTAGATAA CGGGCACTGC GCGGATGTAG CTAGTCCTAT 
CATGGCTTGG CCGCTGGCTC CGGAAAATGG AACTCGGGCT TCGGGGGCAG 
CTCGGCTTTG GGGAACGGCG GCATTTCGTG CTTCGGGACC TCTGGCACAA 
CAGGGTGCGG TAACTCGGGC TCTTTCGGCA CCTCAGGGTG TGGCACATGG 
GGCTCCGTTG GCACCCCTGG CACGGGAGGG TGCGGCAGCT CGTGCTCCTT 
TGGTAC

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wmc(10g1) Plate reference : 10g1
WMC locus number : WMC Length of sequence : 188
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 25 show homology to 3b11 bases 50 to 74 (25 in longest run, maxScore = 52). (GT)10 129 to 148. Duplicate with 3b11, 3b11 has precedence. Bases 113 to 149 show homology to 21d7 bases 181 to 217 (complement) (37 in longest run, maxScore = 56). True duplicate with 21d7, 10g1 has priority.
PCR Comments :



Length of sequence : 188  Consensus sequence is :

..

CAAACCGGCA GATGTTGACA ATGGAGGCCT TGCGGCGGCA GCGGCGGTGG 
CGGTGGGGAC CTTGCTAGGG TTTCGAGCGA GCGAGGGTGT GTCTGTGTGT 
GTGCACGCGC ACGCCCGAGG AGGTTTTGGT GTGTGTGTGT GTGTGTGTGG 
AGGGGGGCGC GGGGTGTTTG AGGAAATGAC ATGAGTAC

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wmc407(10a2) Plate reference : 10a2
WMC locus number : WMC407 Length of sequence : 180
Last updated : Jeu 5 Nov 1998 at 16:28
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)16 100 to 131.
PCR Comments : 61C anneal, polymorphic on acrylamide (136-138 bases).
Forward primer details :
ggTAATTcTAggcTgAcATATgcTc at 30 length 25 bases, Tm 61
Reverse primer details :
cATATTTccAAATccccAAcTc at 143 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 135 bases.



Length of sequence : 180  Consensus sequence is :

..

TGGCCTTTTC TTTTAGAAAA TGTTTTAATG GTAATTCTAG GCTGACATAT 
GCTCACATAA AAGTATTATA TCTAACAGAC ATCTCTATTT TCTGTTGCAG 
AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG ATAGAAGCAA GTGAGTTGGG 
GATTTGGAAA TATGCACATA GAAGATGTAC

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wmc(10b2) Plate reference : 10b2
WMC locus number : WMC Length of sequence : 218
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 218  Consensus sequence is :

..

GCCCTCAGGA CCTTCAAAAG CGCCCGTACT GCCGCCGCCT GCTTCAAAAC 
CTGCTACAAC AGCCAAGGAC CAAAGGAGCA TACAAACACA TCTACTAGAA 
CCCAACGTTC ATTGAGATCC GTAATGGATA TTGAAGAGAC CAGCCACGCC 
GACCTGGAGT CGCTGTCTCA GGACAAAACC CGCCGGCCCC CGCGCCGCCG 
CCTGGCCGGA CCCCGTAC

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wmc(10e2) Plate reference : 10e2
WMC locus number : WMC Length of sequence : 325
Last updated : Lun 5 juil 1999 at 17:23
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 177 to 251 show homology to 8h9 bases 40 to 114 (75 in longest run, maxScore = 97). (GA)9 144 to 161, (GT)8 128 to 143. Duplicate, 8h9 has precedence. Bases 53 to 89 show homology to 25c5.1 bases 68 to 104 (complement) (37 in longest run, maxScore = 85). Bases 179 to 222 show homology to 26g11.2 bases 164 to 207 (44 in longest run, maxScore = 49).
PCR Comments :



Length of sequence : 325  Consensus sequence is :

..

GTGGAGACGG TGTGCGTGTG ATACAGACAT AGAGAGGTGT GAATGTGCAA 
ACGATCATGC ATGAGTGAGA CATAGACAGA TGCCGATACA TTGTGTATAC 
ATGAGGGAAC AGTCTTCTAG TTTACTTGTG TGTGTGTGTG TGTGAGAGAG 
AGAGAGAGAG ATAGAGAGAG AGAGGGAGAG AGAGAGGAGC ATCCGTAACA 
CAACAACCAT CTCTCTCGAT TCGTCCGTCC CTCGCACGAT CGCATGCAAC 
ATATGCATCA ACGCACACAT TTTGACACCC TCACTCAGCC CGTGCCCACC 
TCAAGGCCCG CGCAATCTCT TCGTG

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wmc(10f2) Plate reference : 10f2
WMC locus number : WMC Length of sequence : 214
Last updated : Lun 2 Nov 1998 at 10:28
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed Rsa1 at 3' end, 5' end not located. Bases 198 to 213 (complement) show homology to 17e12 bases 172 to 187 (16 in longest run, maxScore = 31). (CA)6 39 to 50, (CA)12 116 to 139. Too difficult to make primer, microsatellite is embedded in a minisatellite -like repeat.
PCR Comments :



Length of sequence : 214  Consensus sequence is :

..

gttgactTAC TGGCGTTCTC AACAAATACA CCCCCCCCCA CACACACACA 
CGTACCCCCC TAGTTGCGAG TTGATGGTCA ACTTGTAACT GGGGTTCTCA 
ACAAATACAC ACACCCACAC ACACACACAC ACACACACAC CTAGTTGTGA 
GTCGATGGGC AACTTGCAAT TGGGATCCTC AACAAACACA CATCCCGTAG 
TTGCGAGTCG ATGC

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wmc(10g2) Plate reference : 10g2
WMC locus number : WMC Length of sequence : 255
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed Rsa1 at 3' end, 5' end not located. No substantial microsatellite.
PCR Comments :



Length of sequence : 255  Consensus sequence is :

..

cgtggactAT CCATGGCACG GACAGAGCAG CAGAGGCGGG CGCGGCGGGC 
GGCGAGCGGC GGGTCATGGC AGGCGACGnn AACGCGGGCG CCAGTCAGGA 
ACCTCCACGC TCACCATAGC GTGGTCTGCG TCATCGTCCG GCGTCCAGTT 
CAACTCCGTC CAATTCAATT GCCGACACAA CTTAGTTGCC AGGAGCTCGA 
GGTTGGGGTG TCTACGAGCA GTTCGGCCTC GGAGTTGCCC GACGCTGGAG 
CTCGA

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wmc408(10h2) Plate reference : 10h2
WMC locus number : WMC408 Length of sequence : 275
Last updated : Mar 29 DŽc 1998 at 16:10
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 68 to 101 show homology to 9h4 bases 37 to 70 (34 in longest run, maxScore = 26). (CA)29 44 to 101. Bases 43 to 232 show homology to 25c5.2 bases 87 to 276 (190 in longest run, maxScore = 102). Duplicate with 25c5.2, 10h2 has priority.
PCR Comments : 61C anneal, smear (100-140 bases).
Forward primer details :
TAcgTcgATgTgTcgcTcTc at 2 length 20 bases, Tm 62
Reverse primer details :
ATcTcAcTTccTggcTTccc at 173 length 20 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 191 bases.



Length of sequence : 275  Consensus sequence is :

..

GTACGTCGAT GTGTCGCTCT CTCTAACACA CACGCTATTA AAACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC 
ATACACACAC GCACGCACGC ACGCACGCAC ACACGCACAC ATAGGTTCAT 
AGAATAAGAA TATCATGCGA GTGGGAAGCC AGGAAGTGAG ATAAATGGAG 
CATATACAAC AAGAGAAGTG GTGATGAATA TTCCATCAAA CCTGGACTGA 
GGAGTAGATT ATTACTCCCT CCATC

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wmc(10a3) Plate reference : 10a3
WMC locus number : WMC Length of sequence : 320
Last updated : Lun 9 Nov 1998 at 9:50
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. Bases 72 to 124 (complement) show homology to 3d7 bases 516 to 568 (53 in longest run, maxScore = 125). Duplicate, 3d7 has precedence. (GT)4 195 to 202, (GT)6 205 to 216, (GT)5 219 to 228, (GT)6 231 to 242, (GA)6 243 to 254.
PCR Comments :



Length of sequence : 320  Consensus sequence is :

..

gactACAACC TAACTTGGTT ACTCAGCCCA CATTGAGCGA CACCGGAAAG 
GCCCAAACAA GGCTTCCGTA TAACTTTTTG AAGTCACTGA GCTCAATTAA 
TAACTTAAGA AAAAAGTTAG ATTACAGTGG AACCTAATTA AAAGCTGTCA 
CGAGCAAATA AATAATTCAA CGGGTCCCAC TTGTGCATGT GCGCGTGTGT 
GTGCGTGTGT GTGTGTTTGT GTGTGTGTTT GTGTGTGTGT GTGAGAGAGA 
GAGATTGAGA GAGAGACCCA TCGGtCGATG CTCGTAAATA CATCTTTCAG 
CCATATGCAT TGCCGATGCT

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wmc(10b3) Plate reference : 10b3
WMC locus number : WMC Length of sequence : 173
Last updated : Lun 9 Nov 1998 at 9:51
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends ( NB 5' linker corrupted). (CAA)11 32 to 64, (CAA)18 74 to 127, (CAA)14 131 to 172. No room for primer.
PCR Comments :



Length of sequence : 173  Consensus sequence is :

..

ACCACCAACA ACAACCACAC AACAACAACC ACAACAACAA CAACAACAAC 
AACAACAACA ACAACTTTAT CTACAACAAC AACAACAACA ACAACAACAA 
CAACAACAAC AACAACAACA ACAACAACTA CAACAACAAC AACAACAACA 
ACAACAACAA CAACAACAAC AAC

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wmc409(10g3) Plate reference : 10g3
WMC locus number : WMC409 Length of sequence : 660
Last updated : Mar 20 Juil 1999 at 14:57
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 377 to 387 show homology to 13e4 bases 183 to 193 (11 in longest run, maxScore = 49). (CA)10 73 to 92, (CA)13 137 to 162, (CA)6 165 to 176, (CA)12 504 to 527. Several microsatellites in this sequnce, primers are to the last one. NB high bias in bases. Bases 269 to 287 show homology to 10f3 bases 115 to 133 (19 in longest run, maxScore = 65). Bases 4 to 345 show homology to 10h4 bases 1 to 342 (342 in longest run, maxScore = 238). Duplicate with 10h4, 10g3 has priority.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
gAgAcTcTcggAgAAcAcAcAT at 434 length 22 bases, Tm 61
Reverse primer details :
ggTgTgTgTTTTgTTgAggAc at 564 length 21 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 151 bases.



Length of sequence : 660  Consensus sequence is :

..

cgtggactAC AAACACACAC ATCCCTAATT GCGAGTCGAT GGTCGGGTTG 
CAACTGGGGC CATGACAAAA GACACACACA CACACACACA CACCCTTAGT 
TGTGAGTCGA TGATCAGCAT GCAACTAGAG ACCCTGCACA CACACACACA 
CACACACACA CAGACACACA CACACACCTA GTTGTGACTC GATGGTCGGC 
ATGCAACTGG GGAACCTCAA CAAACATCCC TAGTTGCGAG TAGATGGTCG 
GCATGCAACT GGGGGCCTTC GACAAACACA TACACCCTTA GTTGCATGTC 
GATGCCCGGC ATGCAACTGG GGGCTTCGAT AAACACACAC ACATCTAGTT 
GCAAGTCGAT GGTTGGCATG CAACTTGGGA CCCTCGAGAA ACACACACTC 
CCTAGTTTCG AGTCGATGGT TGACATGCAA CCGGAGACTC TcGGAGAACA 
CACATACACC CCTAGTTGTG AGTCAATGGT CGACATACAA CTAGGGGCCT 
CAACACACAC ACACACACAC ACACACACCT AGTTGTGAGT CGATGGGCTA 
GTTGCAACTA GGTGTCCTCA ACAAAACACA CACCCCCTAG TTGCGAGTCG 
ATGCTTGACA TGCAACTGGG GACCCCTTGA CAAAAAGACA CACACACtag 
tccatgcgcc

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