Wheat microsatellite WMC 25/06/01

wmc(10a4) Plate reference : 10a4
WMC locus number : WMC Length of sequence : 463
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 78 to 169 (complement) show homology to 2h11 bases 43 to 134 (92 in longest run, maxScore = 125). (CT)13 1 to 26. Duplicate with 2h11, 2h11 has precedence. Bases 308 to 346 show homology to 26d6 bases 83 to 121 (39 in longest run, maxScore = 95).
PCR Comments :



Length of sequence : 463  Consensus sequence is :

..

CTCTCTCTCT CTCTCTCTCT CTCTCTTTCA CACACACACA CACCACACAC 
ACACTAAGAC TCCATCTCAC ACACACCTGC TCTAGGCGGA GCATTTGTTC 
CTACCACCCT TCATCCAACC TTACCCGTAT GATAAACAAT CACCTTTATT 
TACTTGCATA ACATGGACAG ATTCCACTTT ACTTTAGTAG TCAGCAAACT 
TATCGTCTGT ATACCCTTAT AAAAAAATGA GTTGGCGATG ATGGTGTGCC 
TACCATCTTG TAATACAGAC CGTATGATCT ATATCTGACG GATAGGAAGC 
AAACTAAGAT AATTTTACAA AAGATACCCG CACCTCTCTC CACATCATTA 
TCTCCCGCAA CCTCATTGCT CAGCAATTAA AAAGGAATAA GTCTCTGGAA 
CAATCTAGCA TGCATGGTGG CCGCTGCCGC CTACCGGCGC CGACCATCCC 
CATGCCTnCG CCC

Go back to index

wmc(10f4) Plate reference : 10f4
WMC locus number : WMC Length of sequence : 233
Last updated : Lun 2 Nov 1998 at 9:56
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 132 to 148 show homology to 14f5.1 bases 251 to 267 (17 in longest run, maxScore = 27). No substantial microsatellite.
PCR Comments :



Length of sequence : 233  Consensus sequence is :

..

GTACGCCGGC GTAGAGATGT CGTTCGACGG CGTGTTGTGG AGGAATCGAG 
CGGCGGCGTG GTGAGCAGGC GGCGGCGGCG TGGTGTCGTT CCTCCCAAAT 
CCGCGAGGCC CCCCTCGAGG GTTCTGTACG AACTCTTGAA TAGATCGAAC 
AGAAAGAATA GCTTTGAGGT GGTTTCGTGA CCTACAAACA ATTCCTATCT 
TTTGTTCTCT GCTAATAGGA ACTCGGGAGT GAT

Go back to index

wmc410(10g4) Plate reference : 10g4
WMC locus number : WMC410 Length of sequence : 137
Last updated : Mar 22 DŽc 1998 at 16:02
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)26 69 to 120. Bases 62 to 137 show homology to 2d1 bases 1 to 76 (complement) (76 in longest run, maxScore = 58). True duplicate with 2d1, 10g4 has priority.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
ggAcTTgAAAggAAgcTTgTgA at 21 length 22 bases, Tm 63
Reverse primer details :
cATggATggcATgcAgTgT at 118 length 19 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 116 bases.



Length of sequence : 137  Consensus sequence is :

..

GTACGCACTG TCCATTTCCG GGACTTGAAA GGAAGCTTGT GATGATGCTT 
TTTTGAGTTG CTAGCTTGCA CACACACACA CACACACACA CACACACACA 
CACACACACA CACACACACA CTGCATGCCA TCCATGC

Go back to index

wmc(10b5) Plate reference : 10b5
WMC locus number : WMC Length of sequence : 279
Last updated : Lun 9 Nov 1998 at 11:00
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 82 to 142 (complement) show homology to 2e10 bases 162 to 222 (61 in longest run, maxScore = 127). (CA)9 217 to 234, (CA)9 237 to 254, (CA)8 257 to 272, (CT)8 201 to 216. Duplicate with 2e10, 2e10 has precedence.
PCR Comments :



Length of sequence : 279  Consensus sequence is :

..

TACGAGTTCA TTGGTTTCAA AATAGCCTCA CCCATAGGAT CCACTGCTTC 
CACCCTGATT GTGCCGCCTG CGTGAAATTT TTGTATGCAA GTTCAGTGTG 
CTATGATGTT CTATATGATT GTGTCAACTA TATAAGTTTT TATTGAGCAT 
CACCAATGCA TATGGCTGAA AGATGTATTT ACGAGCATCG ACTGATGGGT 
CTCTCTCTCT CTCTCTCACA CACACACACA CACAAACACA CACACACACA 
CACACGCACA CACACACACA CAGACACAC

Go back to index

wmc(10e5) Plate reference : 10e5
WMC locus number : WMC Length of sequence : 324
Last updated : Jeu 29 Oct 1998 at 16:49
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 324  Consensus sequence is :

..

GTACACAGGG GAGTCCACAC AAGTCCAACT TGGAATTAGA TTGATATGTA 
GTGCGCTTCG TTAACAAGGA CGCCCCGGCC GCAAACAAAT CGCTCCAAAA 
TCGATACTCT CGCCTCGCCG CGCCGCTCCC GTCTCGCGAC GCCGCTGCTG 
CCACCAAAGC CGCGGCCTAT GCATGGCCTA CTGGAGGGTA TAACTCGTTT 
ATCCCGCTCC TACTGTTTTC TGTTTTAGCC ATGCGAGCGT TATACGTAGA 
TCTTTCTGCA ATTAGCGTAG TATGTGCTAG TTTGACTGAA TATCAGTATG 
CGATTATATG TGTCAATGCC ATGC

Go back to index

wmc(10f5.1) Plate reference : 10f5.1
WMC locus number : WMC Length of sequence : 456
Last updated : Lun 9 Nov 1998 at 11:10
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Two inserts in this clone, this is the first insert. Trimmed to Rsa1 at 3' end, 5' end not located. Bases 306 to 346 show homology to 8d1 bases 49 to 89 (41 in longest run, maxScore = 23). (CA)6 306 to 317, (CA)6 334 to 345, (CA)6 349 to 360, (CA)14 371 to 398, (CA)7 433 to 446. No room for primer.
PCR Comments :



Length of sequence : 456  Consensus sequence is :

..

ctgtggactA TCCGATGAGT TCCAATCCTT CCAATCGTTC CAAGTCAAAT 
GCTGAAGATG ATCTGTTTTG GGGCCCATTG ACCACTCCAA ACAAGATAAG 
AAACAGTATG TTCATATGTT CTTTGCAATG GTTTTTATTG TCACTTGATT 
ATTTTGTCAG CGCATATTAT TATCAACAGA AGGGGAGCCT TGGCGCAGTG 
GTAAAGCTGC TGCCTTGTGA CCATGAGGTC ATGGGTTCAA GTCCTGGAAA 
CAGCCTCTTA CAGAAATGTA GGGAAAGGCT GCGTACACGC CCGACCCGCT 
CCATGCACAC ACACACACGC ATACACACAC ATACACACAC ACACACTACA 
CACACACACA TACACACATA CACACACACA CACACACACA CACACACACT 
ACACCAAACA CACAGACACA CACAAACACA CGCACACACA CACACACTAC 
ACGTAC

Go back to index

wmc(10f5.2) Plate reference : 10f5.2
WMC locus number : WMC Length of sequence : 403
Last updated : Lun 9 Nov 1998 at 13:39
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Two inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 95 to 145 (complement) show homology to 6d1 bases 51 to 101 (51 in longest run, maxScore = 109). Duplicate, 6d1 has precedence. No substantial microsatellite.
PCR Comments :



Length of sequence : 403  Consensus sequence is :

..

GGCCCGACCC TTAGGTCTTT GTCATCCTTC CCACTCTATC GTCTTCCTCC 
TCTCGACACC TCAGAGCCCG AGCTCCGCCG CCGCCGCCGC GCATCCCGTC 
TGCACCAACC AAGGCCGCTG CATCGACCTG TCTTGACCAG AGAACTGCGG 
CGCACCTCCG CTGCTCATCA GCACCAGTAA GTCCTCGCCG TCCCGTATAC 
TAGATCCGCA TTAGGGTTCG CGAGTTCTTC TGTGTTCTTC GTGGTTCCTC 
GCGATTCCTT CGCAGTTCTT CCACGACGGC CTCCTTTGAT CCAAGAATAG 
TGCATAACTT CGGCGGTAGT CGTTTCACGC CAATTTTCAA TACTAGCAGA 
TCCCCTTTGC TTGCAAAAAG ACCAAAATTT AGAGCTCATG AACTTCTCTG 
TAC

Go back to index

wmc411(10g5) Plate reference : 10g5
WMC locus number : WMC411 Length of sequence : 341
Last updated : Lun 30 oct 2000 at 16:59
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 10 to 36 show homology to 10g6 bases 13 to 39 (27 in longest run, maxScore = 48). (GT)10 69 to 88, (GT)15 91 to 120. Bases 144 to 169 show homology to TW7b08 bases 16 to 41 (complement) (26 in longest run, maxScore = 32).
PCR Comments : 61C anneal, polymorphic on acrylamide (though weak).
Forward primer details :
gAAcccgTcAcTggAAAcATT at 41 length 21 bases, Tm 63
Reverse primer details :
ccTcgTATTAccATccAgccT at 264 length 21 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 244 bases.



Length of sequence : 341  Consensus sequence is :

..

CTTGCGGGCA TGCTTTGATC GGAAAGCAAC GCATTTTGGC GAACCCGTCA 
CTGGAAACAT TTCTTCATGT GTGTGTGTGT GTGTGTGTGC GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT TGTGTGTGTT TGCGTGTCTC CCGCACGCAC 
ATTATCTTTA GGCCTCTCTC GCACACATTG CCCCCACCCC AGACACACCT 
CTCTCTTAGT AGTTGGCAGA TATGATTCCA TCATATCATT CGGTAGGATA 
TCCCTGACTG TATAGGCTGG ATGGTAATAC GAGGCAAAGT TTTACCCTAG 
TTCGGACCCT TGCAGTTGAG GAAAAAGCCT ACTCCGTGTA C

Go back to index

wmc(10h5) Plate reference : 10h5
WMC locus number : WMC Length of sequence : 339
Last updated : Lun 9 Nov 1998 at 13:50
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Vector sequence. Bases 13 to 338 show homology to 4f1 bases 24 to 349 (326 in longest run, maxScore = 264). Duplicate with 4f1, 4f1 has precedence. No substantial microsatellite.
PCR Comments :



Length of sequence : 339  Consensus sequence is :

..

GCGnGCCTCG nGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGG 
CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA 
ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 
CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 
GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 
TGACTCGCTG CGCTCGGTCG TTCGGCTGCn GCGAGCGGn

Go back to index

wmc412(10a6.1) Plate reference : 10a6.1
WMC locus number : WMC412 Length of sequence : 646
Last updated : Lun 9 Nov 1998 at 14:01
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Two inserts in this clone, this is the first insert. Trimmed to Rsa1 at 3' end, 5' end not located. Bases 459 to 475 show homology to 10f8 bases 112 to 128 (17 in longest run, maxScore = 26). (TTC)5 461 to 475, (GC)7 488 to 554, (GT)27 502 to 555.
PCR Comments :
Forward primer details :
gATccccTccAAAAgTAgcATcT at 424 length 23 bases, Tm 64
Reverse primer details :
cTTcAAcTgccTgcAcAcAAc at 563 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 160 bases.



Length of sequence : 646  Consensus sequence is :

..

cgtggactAA CGCCGTTCCG TGCTATTACT nTGGATAGAC CGGGTGAGCT 
GGTGTGCCTG AGTTAGTAGC AGCGCGACAn CCGTGCCCTG CGCTACCACT 
TGGTCGCACC GCACCGcAAT GTGTTCTCTC CCCTAAGACC GATCGGGCTC 
TCCATCCCAC ACCCCACCCA ATATCTCTCA CGCCACTGCT CCTCCCAATC 
CCATCGTCGA TGCCTCCTCC GCCCCCAAAC TACAGCGATA GGGACGTCGG 
CGCCCTCACC CGTGCCCCCC CCCAACTCGT CTTCTCACCG GTTGTTTGGA 
GGAGCCGCTT TTGCGCTACT GTGGTCGGGC TGCGGCAACC ACCAGAGGTC 
CACCGCGGGC AGGAGCCCCC TCCTTCTnCA CCTGAAACCC TAGCCCGAAT 
TCGGCAGAGG TGCAGCATCA CGGGATCCCC TCCAAAAGTA GCATCTTCTT 
CTTCTGGCTC TTCTTCTTCT TCTTCCCTGG ATCTGATGCG CGCGCGCGCG 
CGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTTCGAG TGGTTGTGTG CAGGCAGTTG AAGGAAGGGG GGCATGGAGA 
AGCTGCAAGG TGCCGCTCCG ATCCTGGCGC CATGGATGGA AGGTAC

Go back to index

wmc(10a6.2) Plate reference : 10a6.2
WMC locus number : WMC Length of sequence : 375
Last updated : Lun 9 Nov 1998 at 14:03
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Two inserts in this clone, this is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)11 5 to 26. No room for primer.
PCR Comments :



Length of sequence : 375  Consensus sequence is :

..

GTacGTGTGT GTGTGTGTGT GTGTGTGCAC TGTCACTCTG TTGTCAGTGG 
GTGTGGTGGT GAGACCTGGA CTGATCCGAA AAGTTTGGAC CCAGCTGGGT 
CTACTCATCC ACTCCCTGCT GGGCACTGCA CTGAATGCCA TTTTTTGCAT 
AGGGTTTGGC TGGCCAGTGG AGGATGAAAG GCGACCAAAT GATTTTTCAG 
TGACCGCGTC TATGTGATGG TGTTATGGGA TCAAGGCATC TCCAATGCGG 
ATCATCAAAC TTTCGCTCGC AAATGTCCAC GGACATCGGT GGGCGCGGAG 
GCCCTCCAAC CGTGAGTAGC ATATGCCGGT CCTTGTCCTT GCCCTCAACC 
TAGGCACACG GGTGACCGGT GCGAG

Go back to index

wmc(10b6) Plate reference : 10b6
WMC locus number : WMC Length of sequence : 429
Last updated : Lun 2 Nov 1998 at 9:57
Owner : Gale, John Innes Date at Agrogene : 15.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Bases 77 to 124 show homology to 10c9 bases 220 to 267 (48 in longest run, maxScore = 121). No substantial microsatellite.
PCR Comments :



Length of sequence : 429  Consensus sequence is :

..

tggactACAC CGTTGTTGAT GGTGGTGTGC CTGCCATCCT GCAATATAGG 
CCGTCCGATT TATATCTGAC AGATATGAAG GAAACAATGG CAATTTTGCA 
AAAAGATACC CACACCCCTC TCCAAATTCG CAAATAAGGC CTTCCCTTGT 
TCATCCTTTT CTCCCACAAG ATAAACTACT CATACAAATG CATCTTGATG 
CGTGCAATGC GTGGGCATCT TGCTAGTTCT AAAAGTCTTT CCATCATTTC 
CCACACTACT AGTTGCAGAT GAAGAACCTA CCCAAGCATA GCGCGATACA 
GGAGCAATGC ACAATTACAA GCTGATATTC TGTTGGACAA TGGAGGCTAT 
AACCAATTAC TTTTACGGGA ACAATAGCAA ACAGGAATTT GAAGGGTCGG 
TATGAACAAA ATTGGAACTG CACATGTAC

Go back to index

wmc(10e6) Plate reference : 10e6
WMC locus number : WMC Length of sequence : 318
Last updated : Lun 9 Nov 1998 at 14:13
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Bases 60 to 291 show homology to 8g12 bases 50 to 281 (232 in longest run, maxScore = 171). (CA)20 251 to 290. Duplicate with 8g12, 8g12 has precedence.
PCR Comments :



Length of sequence : 318  Consensus sequence is :

..

ggcgcgtggc ctAAGATCGA TTGAGCCTAA TAAATCATGT TACCTTTCCA 
ATACAGTCAG GGACCATGCA TCTTATGCCT TCAACAGCTA CTGGCAAAAA 
TACAAGAAAC ATGGTGGTTC TTGCTACTTC AATGCAGCTG CAATGGTCAC 
CGACCTAAAC CCAAGTATGT TCACAACCTA AACCATCATT AGGAGCATAC 
ACTTTTGCAG GTTGAAATTC CACTAGAAAG ATCAAACATG CAGAGCACAA 
CACACACACA CACACACACA CACACACACA CACACACACA CCACAAGCCC 
GCACACACGC ACGTGTAC

Go back to index

wmc(10f6) Plate reference : 10f6
WMC locus number : WMC Length of sequence : 245
Last updated : Lun 9 Nov 1998 at 14:19
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. Bases 148 to 197 show homology to 7d7 bases 126 to 175 (50 in longest run, maxScore = 30). (CAA)6 89 to 106, (CAA)16 149 to 196. Duplicate with 7d7, 7d7 has precedence.
PCR Comments :



Length of sequence : 245  Consensus sequence is :

..

cgtggactAC CACCnCGACA ACGTCAACAA ACAACGACAA CGACAACGAC 
AACGACAATG ACAACGTCAA CAACAACAAC GACAACGACA ACAACAACAA 
CAACAACGAC AACGACAACC ACAACGACAA CGACAACGAC AACGACGACA 
ACAACAACAA CAACAACAAC AACAACAACA ACAACAACAA CAACAACGAC 
GACGACAACG ACAATGACGA CAACAGTAGC AGCAGCAGCA GCAAC

Go back to index

wmc413(10g6) Plate reference : 10g6
WMC locus number : WMC413 Length of sequence : 215
Last updated : Lun 9 Nov 1998 at 14:35
Owner : Gale, John Innes Date at Agrogene : 22.9.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends. Bases 13 to 39 show homology to 10g5 bases 10 to 36 (27 in longest run, maxScore = 48). (GT)9 91 to 108. NB note bias in base frequency.
PCR Comments : tested 51C anneal, no product.
Forward primer details :
cAcTggAAAcATcTcTTcAAcT at 52 length 22 bases, Tm 58
Reverse primer details :
AcAggAAAggATgATgTTcTcT at 192 length 22 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 162 bases.



Length of sequence : 215  Consensus sequence is :

..

GTACTTGCGG GTATGCTTTG ATCGGAAAGC AACGCATTTC GGCGAACCCG 
TCACTGGAAA CATCTCTTCA ACTGTGTGCG TGTGTGTGTT GTGTGTGTGT 
GTGTGTGTGA CTATCTACGT TGTGCATAGC AGCTATATAT ACTGCTGCCT 
GCTGCCGCTT AACGTATACA TGCAACTCAC GTGCGAGAAA AAGAGAACAT 
CATCCTTTCC TGTAC

Go back to index

wmc414(23A1) Plate reference : 23A1
WMC locus number : WMC414 Length of sequence : 172
Last updated : Lun 9 Nov 1998 at 14:44
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 88 to 109 (complement) show homology to 5d5.1 bases 46 to 67 (22 in longest run, maxScore = 21). Homology to one side of microsatellite. Bases 32 to 47 show homology to 24g6 bases 256 to 271 (complement) (16 in longest run, maxScore = 19). (AT)6 53 to 62, (GT)23 63 to 108, (GT)4 111 to 118.
PCR Comments : tested 61C anneal, no product; tested 51C anneal, no product;
Forward primer details :
cgggTAAcAAAATAgTccATcc at 29 length 22 bases, Tm 61
Reverse primer details :
TggTgTATTAgccgTAccAgAg at 146 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 139 bases.



Length of sequence : 172  Consensus sequence is :

..

GGCAAGGGTA ACAGAATAAA TTCTGTTACG GGTAACAAAA TAGTCCATCC 
CTATATATAT ATGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGC GTGTGTGTTC ATTAATGACG CCTTACAACC ATACACTCTG 
GTACGGCTAA TACACCAGGG GC

Go back to index

wmc(23b1) Plate reference : 23b1
WMC locus number : WMC Length of sequence : 181
Last updated : Lun 9 Nov 1998 at 14:46
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 100 (complement) show homology to 14g5.2 bases 55 to 154 (100 in longest run, maxScore = 43), duplicate, 14g5.2 has precedence. (CA)22 63 to 106, (CA)9 135 to 152.
PCR Comments :



Length of sequence : 181  Consensus sequence is :

..

GTCAAGCCAC ACACAAATTC ACCATCAAGC CACACACAGT CAAGCCACAC 
ACACAATCAA GCCACACACA CACACACACA CACACACACA CACACACACA 
CACACAGTCA AGCCACACAT ATTCACCATC AAGCCACACA CACACACACA 
CAGAGTAAAG CCACACATAT TCACCATCAA G

Go back to index

wmc(23c1) Plate reference : 23c1
WMC locus number : WMC Length of sequence : 339
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 339  Consensus sequence is :

..

CATACACTGA CCTCATCCTC ATGGTGCTCC CCTCAGCCTG CTATTTCAAC 
GGACAGGGAG GTCCAGGAAG AAGAAACAAG TGCAAGAACA GAAAAAGGGA 
AAAGAAAACT GAGGTGTATT GATGCCTCGA CGAACCTACG GCCTCGGCAG 
GAGTGAGAGC AGAGACCCGG GCAGCGGCGG CGGCGGCAGC AGAGGACGGT 
CGATGCAGCA CAATACCTCA ACCAGAAGGC GGACACTCCA GCACGGTAGA 
TGAGGAGGCA TGGACACATG ATCCGATAGA GATACTCACG TCGATGAAGA 
CTATGAGACG GCACCGGCAG CGCATGGGGC AGCGGCTGC

Go back to index

wmc(23d1) Plate reference : 23d1
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc415(23e1) Plate reference : 23e1
WMC locus number : WMC415 Length of sequence : 353
Last updated : Lun 9 Nov 1998 at 14:53
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at both ends of insert. (CA)23 271 to 316.
PCR Comments : 61C anneal, polymorphic on acrylamide;
Forward primer details :
AATTcgATAccTcTcAcTcAcg at 184 length 22 bases, Tm 60
Reverse primer details :
TcAAcTgcTAcAAccTAgAccc at 323 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 161 bases.



Length of sequence : 353  Consensus sequence is :

..

AATATTACAG TCAGTTAAAC TCAAAGCATA TCGAGAATCG TTGATGCTAC 
GATCCCGCAG CACTTCCCCG CGTCTCGCGG GGGTGTGGAA CTCGAACCAG 
GAAAATATCT CAGAGAGAAA GGCTTGGAGA AGGGGAAAGA GCAGAGAGGA 
AAGCTTCAGA TGGGGAGTAG TGTTTACAAA GTCAATTCGA TACCTCTCAC 
TCACGCACAC GCTGTCTATA TGTACTACCA TTACAAGCCA CATGGGCCAA 
GCCGGTCCGC TAAGCACACT CACACACACA CACACACACA CACACACACA 
CACACACACA CACACATGGG CCGGGTCTAG GTTGTAGCAG TTGAACAAAT 
ATT

Go back to index

wmc(23f1) Plate reference : 23f1
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23g1) Plate reference : 23g1
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23h1) Plate reference : 23h1
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc432(23a2) Plate reference : 23a2
WMC locus number : WMC432 Length of sequence : 280
Last updated : Mar 17 Nov 1998 at 14:26
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 238 to 265.
PCR Comments : 51C anneal, polymorphic on acrylamide (very complex).
Forward primer details :
ATgAcAccAgATcTAgcAc at 92 length 19 bases, Tm 53
Reverse primer details :
AATATTggcATgATTAcAcA at 261 length 20 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 189 bases.



Length of sequence : 280  Consensus sequence is :

..

CGGAAGCCAT GCACCCCAGC TTGTAGTAGT TGACAACTGT TGTGCGGTGG 
CCAGCCTGCA GCGTCCCCTG GTGAAGTTGC CCCAGCCGCC CATGACACCA 
GATCTAGCAC ACACCCATCC CGAGGCATTA TTGCTAGTCG CGCCTCCGCG 
TGCTTGGAGA GTGACGAGAA TGGGTCGCCA TAGTCGTCGG TTGTGACACG 
GCCGAGGAGG TGGGTCAAGG AGAGGCGCTA GGATCATGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTAATCA TGCCAATATT

Go back to index

wmc(23b2) Plate reference : 23b2
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23c2) Plate reference : 23c2
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23d2) Plate reference : 23d2
WMC locus number : WMC Length of sequence : 167
Last updated : Lun 9 Nov 1998 at 14:59
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (AT)7127 to 140, (GT)6 141 to 152, (GT)6 155 to 167. No room for primer.
PCR Comments :



Length of sequence : 167  Consensus sequence is :

..

GGAAAACTCA CTTTGAAGGC AAGGCTAACA GTGAACTTGC CCAAACATCT 
CAAGTGGAAA ATATATCGGA TGAAGACTGG AGAGGCCTCG TTAAACACTG 
GTCCGATCCG AAGTATCAGT TACATTATAT ATATATATAT GTGTGTGTGT 
GTGCGTGTGT GTGTGTG

Go back to index

wmc(23e2) Plate reference : 23e2
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23f2) Plate reference : 23f2
WMC locus number : WMC Length of sequence : 279
Last updated : Lun 9 Nov 1998 at 15:07
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 134 show homology to 11f12 bases 433 to 566 (134 in longest run, maxScore = 97). (GT)8 265 to 278. Duplicate with 11f12, 11f12 has precedence.
PCR Comments :



Length of sequence : 279  Consensus sequence is :

..

GTGTGTGTGT TTGTTGACGA GCCCAGTTGC AAGTTGACCA TCAACTCGTA 
ACTAGGGGGT GTGTGTGGGT GTGGGTGTCA AGGGTCCCCA ATTGCATGTC 
GATAATCGAC TTGCAACTAG AGATAGTGTG TGTGCGTGTG CGTGCGTGTG 
TGTTTTATTT GTAGCCCCCT GTCTTGCCAG GTGACCACCA ACTCGCAACT 
AGGGGCGTGT GGGTGTGTGT ACCAAGGGTC CCAATTCCAT GTCGATAAAT 
GACTTGTAAC TAGGGTGTGT GTGTGTGTG

Go back to index

wmc(23g2) Plate reference : 23g2
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23h2) Plate reference : 23h2
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc416(23a3) Plate reference : 23a3
WMC locus number : WMC416 Length of sequence : 541
Last updated : Lun 9 Nov 1998 at 15:14
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at both ends of insert. (CA)11 239 to 260, (CA)11 263 to 284, (CA)8 287 to 302.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
AgcccTTTcTAccgTgTTTcTT at 169 length 22 bases, Tm 62
Reverse primer details :
TATggTcgATggAcTgTcccTA at 367 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 220 bases.



Length of sequence : 541  Consensus sequence is :

..

AATATTTGTA ATCTTTTTTT ATGAATTGGT AATCCCCATG ACAAGCGGGA 
TCATCAAATC GGCATATTGT ACTATCTATT GTTGGATGAG TTTTCCTTGA 
TTAACTCTTG GAAACCAAGA TTGCATTTTA CTATCAGTAG TATCACATTG 
CAATCGCAGG GCATACTAAG CCCTTTCTAC CGTGTTTCTT TATTATTCTG 
ACGCCTACAT TTCTATCTAC CGCAATAATC ACACCCAACA CACACACACA 
CACACACACA CGCACACACA CACACACACA CACACGCACA CACACACACA 
CAAATGGTGT GATTGTTCTC TAACTGTATT TCCCATATAT AGAAGCTTCA 
CAGTGGTTGG AAATTTTAGG GACAGTCCAT CGACCATAAT CTGACGGTTG 
TAAAATAGAT ACCAGACTGA AAATACCAGT ACTTATGGGC CGGTACTACC 
TTTTTTCTGA GGTTGTTTGA TTCACCCACC CCGTGTATTC TCGCCGGCAT 
GCGGCCGAGT CAACGGTGCC GAAAACCTTC GCCAAAATAT T

Go back to index

wmc(23b3) Plate reference : 23b3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23c3) Plate reference : 23c3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23d3) Plate reference : 23d3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23e3) Plate reference : 23e3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23f3) Plate reference : 23f3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23g3) Plate reference : 23g3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23h3) Plate reference : 23h3
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23a4) Plate reference : 23a4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23b4) Plate reference : 23b4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23c4) Plate reference : 23c4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23d4) Plate reference : 23d4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23e4) Plate reference : 23e4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23f4) Plate reference : 23f4
WMC locus number : WMC Length of sequence : 191
Last updated : Lun 9 Nov 1998 at 15:16
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 11 to 66 show homology to 5e3 bases 164 to 219 (56 in longest run, maxScore = 86), duplicate, 5e3 has precedence. (GT)9 49 to 66.
PCR Comments :



Length of sequence : 191  Consensus sequence is :

..

GTGGTCGCCA TATAACCTTG GATGGGGATG AATTGTGTGC TCGGGGGAGT 
GTGTGTGTGT GTGTGTTGTG TTGCGTGTGT GTGTGTGTAT GTGTGTGTGT 
GTGTCAGATA TTGAGCGAAA ACAAACCTAA GGAGAGAAAT GGGTATTCAC 
GAAGAGATTG CGCGGGCCCT TAGGTGGGAA GGGGCCGAGT G

Go back to index

wmc(23g4) Plate reference : 23g4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23h4) Plate reference : 23h4
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23a5) Plate reference : 23a5
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(23b5) Plate reference : 23b5
WMC locus number : WMC Length of sequence : 299
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 144 to 194 (complement) show homology to 6c6 bases 50 to 100 (51 in longest run, maxScore = 133), duplicate, 6c6 has precedence. (GT)17 145 to 178. Bases 196 to 233 show homology to 20g7 bases 179 to 216 (38 in longest run, maxScore = 71).
PCR Comments :



Length of sequence : 299  Consensus sequence is :

..

TGCACACAAA TTTTGGGCAC TTGTATTCGA CTTAAGTCGG TGTGCCACCG 
TATCTCATAT ACTTACTACT CCCTCCGTCT AGGTGTAATA AGTCACGTTA 
GAAGGTGCAA CGGGACCAAG GTGCGTGCGT GCGTGTGTGT GTATGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTTT AACACCATGT GTGTATTAGA 
GAGAGCGACA GATCGACCTA CTCCTACAGA GAGATGTTGT GTAGTGTACG 
ATTTTAGCCG CGAGAGTCGG TGCATCCTCT CGTTTCCGGG CGTGTCCGG

Go back to index

wmc(23c5) Plate reference : 23c5
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:50
Owner : Romestant, RAGT Date at Agrogene : 29.9.98 Public Domain : Yes
Comments : No sequence 29.9.98.
PCR Comments :
No sequence available for this clone
Go back to index