Wheat microsatellite WMC 25/06/01

wmc(6d1) Plate reference : 6d1
WMC locus number : WMC Length of sequence : 212
Last updated : Lun 2 Nov 1998 at 9:57
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR52. No substantial microsatellite. Trimmed to insert. Bases 51 to 101 show homology to 10f5.2 bases 95 to 145 (complement) (51 in longest run, maxScore = 109), duplicate, 6d1 has priority.
PCR Comments : 


Length of sequence : 212  Consensus sequence is :

..

CGGGACGACG AGGACTTACT GGTGCTGACG GGCAGCGGAG ATGCGTCGCG 
GTTCTCTGGT CAAGACAGGT CGATGCAGCG GCCTTGGTTG GTGCAGACGG 
GTTGCGCGGC GGCGGCGGCG GAGCTCGGGC TCTGGGGTGT CAAGAGGAGG 
AAGACGATGG AAGGGGAAGA GTGACGAAGA CCTGAGGGCC GGGCCTATTT 
ATAAGGGATG GC
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wmc42(6e1) Plate reference : 6e1
WMC locus number : WMC42 Length of sequence : 284
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR53. Trimmed to insert . (GT)8 202 to 217. Polymorphism is in very large fragments (~400) , ~4 loci.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
gcccTTggTccTggggTgAgcc at 52 length 22 bases, Tm 76
Reverse primer details :
gccTcATccAgAgAgccTgcgg at 259 length 22 bases, Tm 73
Expected size of amplified fragment (Chinese Spring=) 229 bases.


Length of sequence : 284  Consensus sequence is :

..

GTACGAGCTC TTTAGCTCAT GGCCGCGCCC TTGCCGGCCA CCCGCGTCCG 
CGCCCTTGGT CCTGGGGTGA GCCCATGGCC CCCCTGCCCG CTGTTACTGC 
TACCTGCTGA TGCTCCCTGC TACCGCTCTC TGCTGCTGGC TGCTCATGCG 
CTGACCTTGG CTGTGGCCGT TGCCATGGCC GAGCGCCCCT GTTCTCTATG 
CGTGTGTGTG TGTGTGTTGT GTGCATCCAG CGTGTGTGTG TAAGTGTGTG 
CGTGGTGGCC GCAGGCTCTC TGGATGAGGC CGGC
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wmc(6f1) Plate reference : 6f1
WMC locus number : WMC Length of sequence : 68
Last updated : 15 Nov 1996 at 19:09
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR54. Trimmed to fragment. (GT)16 8 to 39. No room for primers.
PCR Comments : 


Length of sequence : 68  Consensus sequence is :

..

GGTTTTGGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG GAGGGGGCGC 
AGGGTGTTTG AGGAAATG
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wmc(6g1) Plate reference : 6g1
WMC locus number : WMC Length of sequence : 502
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR55.(GA)8 150 to 165, (GA)9 205 to 222, (GT)8 321 to 336, . Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 9H3, 6f11 (1). Bases 227 to 248 show homology to 2e4 bases 92 to 113 (complement) (22 in longest run, maxScore = 28).
PCR Comments : 


Length of sequence : 502  Consensus sequence is :

..

TGGAATTTGT CCATGTTATG CAAGTAAATA AAGGTGATTG TTTATCATAC 
GAGTAAGGTT GGATGAAGGG TGGTAGGAAC AAATGCTCTG CCTAGAGCAT 
GTGTGTGTGA GATGGAGTCT TAGTGTGTGT GTGGGGTGTG TGTGTGTGTG 
AGAGAGAGAG AGAGAGGAGA GAGAGAGATG GAGTCTTAGT TTGTGTGTGT 
GAGTGAGAGA GAGAGAGAGA GATGGAGTCT TAGTGTGTGT GGGGGGGGTG 
TGCGTGCGTG CGTGCGTGTG TGTGTGAGAG AGAGATAGAG ATGGAGTCTT 
AGTGTGTGTG GGGGGGGTGC GTGTGTGTGT GTGTGTCGCG GGGTCCTCTG 
TGGCGGATCT GAATATGCTA TCCACTCATC TTTCACATGC CTCTAGCATG 
ATGAATATGG AACCTTACCC TCCAGATCGT CTGACCGATA AATTCCTAGA 
GCCGGGAAAA TATTCCCGAG TATTTTCCCG CTCCACCTAT AACACTTAGT 
AC
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wmc(6h1) Plate reference : 6h1
WMC locus number : WMC Length of sequence : 66
Last updated : Wed, Aug 6, 1997 at 10:00 am
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR56. Mostly vector sequence - trimmed at 5' (corrupted linker) 6.8.97.
PCR Comments : 


Length of sequence : 66  Consensus sequence is :

..

GATAGGATTG CGAACTCTTT GTTTATTGCA CGACAAACTA CATAGATTAG 
TATTTGTTAG TCCACG
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wmc(6a2) Plate reference : 6a2
WMC locus number : WMC Length of sequence : 341
Last updated : Mer 14 juin 2000 at 10:35
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR57. No substantial microsatellite. Bases 71 to 106 show homology to TW1b11.1 bases 81 to 116 (complement) (36 in longest run, maxScore = 71).
PCR Comments : 


Length of sequence : 341  Consensus sequence is :

..

AGGACAAGAC CAATCCTAGC ATAGCACAAG ATCGTGTAGT TCGTTTTGCT 
AGAGCTTTTC CAATGTCAAG TATCTCTTCC TTAGACCATG AGATCGTGTA 
ACTCCCAGAT ACCGTAGGAG TGCTTTGGGT GTACGACTTC GTTGGTTTCA 
ACATAGCCCT TCCCGTAGCG ATCCACTGCT TCCATCCTGA TTGTGCCGCC 
TGCGTGAAAT TTTTGTATGC AAGTTCAGTG TGCTATGTGT TGGGGGAACG 
TAGCAGAAAT TTAAAATTTT NTACGTATCA CCAAGATCAA TNTATGGAGT 
CATCTAGCAA CGAGAGAGAG GGGAGTGCAT CTTACATACC C
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wmc43(6b2) Plate reference : 6b2
WMC locus number : WMC43 Length of sequence : 570
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR58. Trimmed to insert at 3' end. (GT)14 429 to 456.
PCR Comments : 61C anneal, product; 51C anneal, product;
Forward primer details :
TAgcTcAAccAccAcccTAcTg at 194 length 22 bases, Tm 63
Reverse primer details :
AcTTcAAcATccAAAcTgAccg at 493 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 321 bases.


Length of sequence : 570  Consensus sequence is :

..

AACCAACGTC TCTTCCTAGT GGGCAAAGAT GTGAGGATCA TGTGAGAGAG 
TAGTCTGTCG TATTGTGCCA TTTGGTCATG GCCTACCCTT CCTATATATG 
GCAATGCCAC CGCAGAGGCC GATCCATCCA TCTAGTAGCA GCAGTGGAAG 
AGCAGATCAG GGAGCCTTCC TTCAAACTCG CCACCTAGGC ACCTAGCTCA 
ACCACCACCC TACTGCTCAG CCATGAACAA GGTGAAGAGC CATGGCGGCA 
AGCACAAGGG GTCAGGAGGA GGAGGGGGAG GAGGGATCAG CAGGATGCTG 
AGGCAACACA AGGACAGGCT CTACATCATC CGGCGATGCG TCGTCATGCT 
CCTCTGCTAC CATGACTGAC CCTACATTGG TTCTCGTTTG TAGTTAGGCT 
AGTTAGGCTT AGACCGATTC TCTGTGATGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTTCAT AGGGTAGTGT ATGTTTAGAT AGATTTCGCT GACGGTCAGT 
TTGGATGTTG AAGTTTTGTG TGGTGTCATG GTTCCCCTAA GTCCTGCAAC 
GGCAAGGATG TGCATCTGAG
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wmc(6c2) Plate reference : 6c2
WMC locus number : WMC Length of sequence :
Last updated : 14 Nov 1996 at 12:09
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR59 - no sequence available from Lark
PCR Comments :
No sequence available for this clone
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wmc(6d2) Plate reference : 6d2
WMC locus number : WMC Length of sequence : 0
Last updated : 15 Nov 1996 at 19:09
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR60. No overlap between forward and reverse sequences. No insert.
PCR Comments : 


Length of sequence : 0  Consensus sequence is :

..

CCTCGAGGCG CGCCTCGAGG GATCCTCTAG AGTCGACCTG CAGGCATGCA 
AGCTT
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wmc44(6e2) Plate reference : 6e2
WMC locus number : WMC44 Length of sequence : 531
Last updated : Thu, Jun 11, 1998 at 11:27 am
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
PIC Value : 0.73
Comments : AGR61. Trimmed to Rsa1 at 3' end. (GT)35 341 to 410. 5 polymorphic loci.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
ggTcTTcTgggcTTTgATccTg at 237 length 22 bases, Tm 66
Reverse primer details :
TgTTgcTAgggAcccgTAgTgg at 457 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 242 bases.


Length of sequence : 531  Consensus sequence is :

..

AACAACCTTT TGCTCCCCCC TCTCGTGAAA GGGACGTTAG GGCGAACAAC 
TCATTCCTTC TGGGCTCTGT CGGACAACCC GTAAACTCAC CTCCCCTCTG 
CATGTTACTG CGATGGTCGC TACGGGGAGG GGAATCCAAA TGCATCTGTT 
CCGACGGCTA GTAGTATGTG TTATGCTTTT TTTTTAGTTT TTGCAGGGGC 
AGCGCTCGAG TGGATGCAAC GTTTCATCTT TGAGTCGGTC TTCTGGGCTT 
TGATCCTGCT CGAATCAGTT CATCGAAACG GACTCAGCGG AGCTCTAGGA 
TAAACTCCCG CCGTATGCCG TGAGGTTACG GTTTCTCGTC GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GACCTGGTGT CAGGCATTGA ATCGATTTTT AAGGGTTCAA 
CATCGGCCAC TACGGGTCCC TAGCAACACA TCCATGAAGA CTTCCCGATG 
GTCATCAACA AGATCAAGCC GACTCTGGTAC
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wmc(6f2) Plate reference : 6f2
WMC locus number : WMC Length of sequence : 190
Last updated : 15 Nov 1996 at 19:15
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR62. Trimmed to Rsa1. Complex microsatellite 189-361 . No room for primers.
PCR Comments : 


Length of sequence : 190  Consensus sequence is :

..

AGCACACACA TATGTAGCAG CACACACACA CACGCACACA CGGACACAGC 
AGCACACACA CGCAGCACGC ACGCACGCAC GCACGCACAC AAACACGCAG 
CAGCACACAC ACGGATGCAG CAGCATACAC ACACAGCACG CACACACACA 
CAAACACGCA GCAGCGCACA CACACGGACG CAAGCAGTAC
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wmc(6g2) Plate reference : 6g2
WMC locus number : WMC Length of sequence : 976
Last updated : Wed, Aug 20, 1997 at 1:49 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR63. Trimmed to insert at 3' end, insert starts around base 18. No overlap between forward and reverse sequences. Complex microsatellite at end of sequence. No room for primers. First 90 bases match 6c8. Bases 23-104 (ie. 5' side of microsatellite) match part of 3d6.
PCR Comments : 


Length of sequence : 976  Consensus sequence is :

..

GAATTCCTCG AGGCGCGTCT GGCCGGCGGA TCTTTTTTTC ATACACACAC 
GCATACACAC ACATACACAC ACACTACACA CACACATACA CGCACACACG 
CATACTAGNC TGGCCCCCTG AATCTCCAGA CAACCAATAT CACTTAAATA 
AGTGATAGTC TTAATACTAG TTTTTAGACT AGTCATTGGA GAACAGATGA 
TTGATGTCTT AGGGCCGGAG AAACGCAGAC GGCGTACCAC ACAGGAAAAG 
ATCGCAATTG TTCAGCAGAG CTTTGAACCG GGGATGACGG TCTCCCTCGT 
TGCCCGGCAA CATGGTGTAG CAGCCAGCCA GTTATTTCTC TGGCGTAAGC 
AATACCAGGA AGGAAGTCTT ACTGCTGTCG CCGCCGGAGA ACAGGTTGTT 
CCTGCCTCTG AACTTGCTGC CGCCATGAAG CAGATTAAAG AACTCCAGCG 
CCTGCTCGGC AAGAAAACGA TGGAAAATGA ACTCCTCAAA G
(break)
CAACAGTGAA ACAGTACAGG ACGTCATGCT GGGAGCGGTG GAACGCCGCT 
TCGGCAACGA TCTTCCGTCG TCTCCAGTGG AGTGGCTGAC GGATAATGGT 
TCATGCTACC GGGCTAATGA AACACGCCAG TTCGCCCGGA TGTTGGGACT 
TGAACCGAAG AACACGGCGG TGCGGAGTCC GGAGAGTAAC GGAATAGCAG 
AGAGCTTCGT GAAAACGATA AAGCGTGACT ACATCAGTAT CATGCCCAAA 
CCAGACGGGT TAACGGCAGC AAAGAACCTT GCAGAGGCGT TCGAGCATTA 
TAACGAATGG CATCCGCATA GTGCGCTGGG TTATCGCTCG CCACGGGAAT 
ATCTGCGGCA GCGGGCTTGT AATGGGTTAA GTGATAACAG ATGTCTGGAA 
ATATAGGGGC AAATCCACAT ACACACATAC ACACACACAC ACTACACAAA 
CACATACATG CACACACGCA TACACACATA CACAC
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wmc45(6h2) Plate reference : 6h2
WMC locus number : WMC45 Length of sequence : 342
Last updated : Mar 30 mai 2000 at 9:54
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR64. Trimmed to Rsa1 at 5'. (CT)17 144 to 177. (Several loci). 3' PRIMER IS WITHIN VECTOR SEQUENCE (LINKER DISRUPTED) - why does this PCR work?
PCR Comments : 61C anneal, product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
TggAgAggTggAgTggAgTggg at 119 length 22 bases, Tm 70
Reverse primer details :
gAcggccAgTgAATTccTTgAg at 250 length 22 bases, Tm 67
Expected size of amplified fragment (Chinese Spring=) 153 bases.


Length of sequence : 342  Consensus sequence is :

..

GTACCTGCCT GCCTGCGGCG GCGCCCCACG GCGGACGGGC GGCGGAGGGA 
GAGTTAGGGT TAGGCGAAGC AGGAGGGCTC CGATTCGAAG GAGCCACTCG 
GCGCGGCGAC CTGCGGAGTG GAGAGGTGGA GTGGAGTGGG GCGCTCTCTC 
TCTCTCTCTC TCTCTCTCTC TCTCTCTGCC TCCCTCCCCG TCTCTCCTCG 
GCGAGGTGGC GACCTGCGTG CTCTGGTCGG GGTGGATTAT TCCACGCGCC 
TCAAGGAATT CACTGGCCGT CTTTTTACAA CTTCTTGACT GGGAAAACCC 
TGGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TT
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wmc(6a3) Plate reference : 6a3
WMC locus number : WMC Length of sequence : 293
Last updated : Lun 9 oct 2000 at 14:42
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR65. Trimmed to insert at 5' end and at 3' end.(GA)16 44 to 75, (GA)9 132 to 149, (GA)8 231 to 246. No room for primers. Core is similar to 7B4. Bases 107-265 match 3b6. Bases 72 to 98 show homology to TW2g01 bases 111 to 137 (complement) (27 in longest run, maxScore = 20).
PCR Comments : 


Length of sequence : 293  Consensus sequence is :

..

ATGCGAAGGA AGGGAAAGGG AGAGGGAGAG GGAGAGGGAG AGGGAGAGAG 
AGAGAGAGAG AGAGAGAGAG AGAGAGGGAG AGGGAGAGAG AGAGAGAGGG 
AAGGAGAGAG AGGGAGAGAG AGAGGGGGGG GGAGAGAGAG AGAGAGAGAG 
GGAGAGAGAT GGAGAGAAAG GGAGTGGGAC CAACAAGAAT TTTGTGTTGG 
ATGCATGCGA CAGAATTGAA GATTGTCGGT GAGAGAGAGA GAGAGAGGGA 
GAGAGAGAGG GAGAGAGAGA GGGAGAGAGA GGTANTCCAC GCG
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wmc(6b3) Plate reference : 6b3
WMC locus number : WMC Length of sequence : 242
Last updated : 14 Nov 1996 at 19:25
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR66. Trimmed to insert at 5' end. (GT)17 39 to 72. Too difficult to get design primers with matching Tms.
PCR Comments : 


Length of sequence : 242  Consensus sequence is :

..

GTTCATATGT TAGGGTATTG TAAGAGATCT TACACATTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTATAGAGAG AGAGTAACAC TATTCTAATC 
CGGGGATCAA AATAGTTATT TGGATCACAC AGCTCTATAT AGGGCCTGAC 
GATGCGTTCC TGAACTCCAA AAACACAAAA GAACAAAACC TAGATCGCCC 
ATCAGACCCG TCCGACCGGT TTCATCCCCA CACCATGAAC TT
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wmc(6c3) Plate reference : 6c3
WMC locus number : WMC Length of sequence : 182
Last updated : 14 Nov 1996 at 19:27
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR67. Trimmed to insert. (CA)22 82 to 125. Too difficult to design primers.
PCR Comments : 


Length of sequence : 182  Consensus sequence is :

..

GTACATACAT AAAATGGATA CATACACAAG CAATTTGGTT CAGTTTCAGT 
CATCCGAAAC TCGACCAAAA CGTGATGCTT ACACACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACCATA GTTTTCTTCA AAAGTATGGC 
CATTTTAACT CCACAAGTAT GGCTGGTAAT AC
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wmc46(6d3) Plate reference : 6d3
WMC locus number : WMC46 Length of sequence : 516
Last updated : Tue, Aug 26, 1997 at 5:49 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR68. Multiple inserts (2) joined 227-240 by linkers. Complex microsatellite 97-198 in first insert. (>2 loci). Internal portion of second insert is duplicated in 3H12. Bases 243 to 311 show homology to 17f9 bases 15 to 83 (complement) (69 in longest run, maxScore = 58).
PCR Comments : tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
AATccTgTTTTccATgcTcgTg at 67 length 22 bases, Tm 65
Reverse primer details :
gAcgAATcgAgAgAgATggTTg at 185 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 140 bases.


Length of sequence : 516  Consensus sequence is :

..

GGGAGTCTCT AAAGAGCAGT CACCGGTCTT TGGTGTGATA ACATTGGTGC 
GACGTATCTT TCATCTAATC CTGTTTTCCA TGCTCGTGAA AGAACAGTCT 
CCTAGTTTAC TTGTGTGTGT GTGTGTCTGT GTGTGTGAGA GAGAGAGGGA 
GAGAGAGACA GAGAGAGGAG CATCCGTAAC ACAACAACCA TCTCTCTCGA 
TTCGTCCGTC CCTCGCACGG TTGCTAGTCC ACGCGTGGAC TANAGTGCTG 
GATGGGTGAT GGAGTTGCTT CTCGAAGATG GTGGGAGAGG CCTACTANAC 
AAACTGAGGG TGGAACTGCA ATATTATCAA TAAGAGTGGG GATGTGTTTC 
TGTGTGTGCC TGTGCGTGAT AGATCTGTTG GGACGCATCC ATGGATGATG 
AAGGGGAACC ATGTGTTTGG TAAGCAAACC TAACTAGATT GGTAGATCAA 
TTGTTGTTTG TCGGAGGAAG GGAGAGACGT AACTAATGAG GTAGATCGAT 
CGACGCGCCT CGAGGG
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wmc(6e3) Plate reference : 6e3
WMC locus number : WMC Length of sequence : 295
Last updated : 15 Nov 1996 at 19:23
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR69. Trimmed to insert. (CA)10 19 to 38. Not enough room for primers. Relative 6a5
PCR Comments : 


Length of sequence : 295  Consensus sequence is :

..

GGAGGCACAC ACACACACCA CACACACACA CACACACATT GTTTTTACTT 
ATTCTCTATT TTCTGTTGTT TAGACACAAA TATGATTTTT CAAACCGTTA 
TGGTTAGGCG TTGCCGCTCC TCCACCAACA CCACGCCGTC GTGCTACTGC 
TGGACAGAGT CTTCCCCAAC CTCTCCCTCT CTCCTTGCTA GATCAAGGCA 
TGGGCGACGT CACTGGGCTG CACGTGTGTT GAACGCGGAG GCACCGTTGT 
TCGGCGCTTA GATCAGAATC GACCGCGATC TGAATCGCCA CGAGC
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wmc47(6f3) Plate reference : 6f3
WMC locus number : WMC47 Length of sequence : 395
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
PIC Value : 0.39
Comments : AGR70. Trimmed to insert at 3' end. (CA)8 86 to 101. (3 loci).
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Forward primer details :
gAAAcAgggTTAAccATgccAA at 30 length 22 bases, Tm 65
Reverse primer details :
ATggTgcTgccAAcAAcATAcA at 149 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 141 bases.


Length of sequence : 395  Consensus sequence is :

..

AAAAATCGTG TGATCATTAT TGGCCTATCG AAACAGGGTT AACCATGCCA 
AAACATTATC ACCTCAGCGA GTAAGGAGGA CCCCCCACAC ACACACACAC 
AGACACACAC ACAACCACCA CCACAAATCA TAATGCACCT CGACAACATG 
TATGTTGTTG GCAGCACCAT TGACTATCCA GATTTGATTT CTTTGCGGTC 
ATTTCCCATT ACGGTTGGTT TGTATACAAT TTCTAAATTT GAGGAACAAA 
AAAATCATCC AATTAAATTT TTCAAATTTG TAGTTTTTCC ATAACACGTA 
TTTGAGTTAC CTCGCCTTTT TATAGAGAAT GACAACACTT CATTGATTAA 
TAAACACTGT TACAAAGTTC ATCCTGGATA CATTCTGAAA TCACA
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wmc(6g3) Plate reference : 6g3
WMC locus number : WMC Length of sequence : 366
Last updated : 14 Nov 1996 at 12:09
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR71. Trimmed to insert at 3' end. No substantial microsatellite.
PCR Comments : 


Length of sequence : 366  Consensus sequence is :

..

AAAGTCGGCG GGGATGATGT GCATGGCAGG ATGCCTCGTC GTCTCCCTCC 
CCCACAGGGG CTTGAAGTAG TCCCTGAGGT GGACCACGTT GACCATCGAA 
GGGAAGTAGG CGTGCTGCAT CCGAAGCATC GCCTCGCTNT CCGGCGCCTC 
CTTCCCCCCG GCATCCTTGG CCACTCCCTT TCCCTTGCTA GTTTTTGGTG 
CCATGGCGGC TGANAAGGAG GTAAGGGATC AANANCTATG GGGTTGCGGC 
GGCGGCGANC GGAGGCAAGG ACTTCGATTT CTCGCTTGAA GGGGGAGAGG 
GGAACGACGG TTCCGAGATT GGAAAAGACG CAGAGGGTAA ATGAGCAGCG 
CGTCTGCGAT TTTCCC
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wmc(6h3) Plate reference : 6h3
WMC locus number : WMC Length of sequence : 265
Last updated : Lun 4 Jan 1999 at 10:32
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR72. Trimmed to insert. (CA)14 61 to 88. Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 9H3. No room for primers. Bases 159 to 265 show homology to 20A7 bases 133 to 239 (complement) (107 in longest run, maxScore = 129).
PCR Comments : 


Length of sequence : 265  Consensus sequence is :

..

CACACACTAA GACTCCATCA CACACTAAGA CTCCATCTCT NTNTNTCACA 
CACACACACC CACACACACA CACACACACA CACACACAAA CACACACCCA 
CACACAGACA GACTCCATCT CACACACACA TGCTCTAGGC GGAGCATTTG 
TTCCTACAAC CCTTCATCCA ACCTTACCCG TATGATAAAC AATCACCTTA 
ATTTACTTGT ATAACATGGA CAAATTCCAC TTTACTTTAG TAGTTAGCAA 
ACTTATCATC TGTAC
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wmc(6a4) Plate reference : 6a4
WMC locus number : WMC Length of sequence : 285
Last updated : Tue, Aug 5, 1997 at 5:23 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR73. Trimmed to insert at 3' end. (CA)9 30 to 47. No room for primers. Shares homology to 1g1 family.
PCR Comments : 


Length of sequence : 285  Consensus sequence is :

..

TCATTTCCTC AAACACCCGC GCCCCCCTCC ACACACACAC ACACACAACA 
CACACACACC CAAAACCTCC TCGAGCGTGC GCGCACACAC ACACCCCCTC 
CCTCACTCGA AACCCTAGCA AGGTCCCCGC CGCCGCTGCC GCCGCCGTTG 
CTGCCGCAAG GCCTCCATTG TCAACATCTG CCGGTTTGCC CATGCATCTT 
ACCCACCGAT CTCCATACCT AGGCCGCCCT GAGTTTCTTA GATGACGCCT 
GCCAAACGCC CCCTTTCCCA CAGATCCCCA TCCCC
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wmc(6b4) Plate reference : 6b4
WMC locus number : WMC Length of sequence : 243
Last updated : Jeu 27 Aož 1998 at 12:03
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR74. Trimmed to insert. No substantial microsatellite. Bases 77-243 match (mostly) part of 3H10. Bases 211 to 241 show homology to 10A7 bases 490 to 520 (31 in longest run, maxScore = 68).
PCR Comments : 


Length of sequence : 243  Consensus sequence is :

..

GTACACTGTC AGGGTTCTTC GCCTACTTCG AGCACCGCCG CCGCACTCCT 
AACCTAGCCG ATGCCGCCGC CGTCAGGCCG CTGCCGCCGC TNTTCTTTGG 
CCACTGCCGC CGCTATCTTN GTAGCCGCTG CCACCCGTCC ACCCCCTTCG 
TNTTTGTCCA AGCACCAGCC CGTCGCCAGC GTCGCCGTCA TNTACCCCGA 
CCACTTCGTT TACTCCGACC ACCATTGGTG ACATCGGCCC CGC
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wmc48(6c4) Plate reference : 6c4
WMC locus number : WMC48 Length of sequence : 227
Last updated : Thu, Jun 11, 1998 at 11:28 am
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR75. Trimmed to insert. (GA)9 177 to 194. Not sure if polymorphic. Contains sequence element 104-172 that matches complement of 6a12. Jorge Dubcovsky maps this to all group 4 homoeologues.
PCR Comments : 61C anneal, product;
Forward primer details :
gAgggTTcTgAAATgTTTTgcc at 101 length 22 bases, Tm 64
Reverse primer details :
AcgTgcTAgggAggTATcTTgc at 202 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 123 bases.


Length of sequence : 227  Consensus sequence is :

..

GTACATGCCC TAAGTTGTGC CATAAGATGC TTCACTAAAA CATAATCTCT 
TCACTATCCA TGGAACAAGA ATAACCAAAT GACAATGTAA ATCGGGGATA 
GAGGGTTCTG AAATGTTTTG CCTCCCAAGA CGAAATAACT CAATTTTTGT 
GCGTGTGTGT GCGTGTGTGT GTATGTGAGA GAGAGAGAGA GAGAGGGATA 
GGCAAGATAC CTCCCTAGCA CGTGGAC
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wmc49(6d4) Plate reference : 6d4
WMC locus number : WMC49 Length of sequence : 333
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR76. Trimmed to insert at 5' end. (CT)19 141 to 178.
PCR Comments : tested 61C anneal, no product; 51C anneal, product;
Forward primer details :
cTcATgAgTATATcAccgcAcA at 82 length 22 bases, Tm 60
Reverse primer details :
gAcgcgAAAcgAATATTcAAgT at 266 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 206 bases.


Length of sequence : 333  Consensus sequence is :

..

GTACACTTTG AGATAGACTT ATATATCCAC CAGGCAAATG CTACTAGGTA 
GATCATCTGA TAGCTATGTA TGTAGTCATG ACTCATGAGT ATATCACCGC 
ACACCTAACT ACCTGCAATC TTGTTCATGG TGTATAGCGT CTCTCTCTCT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTTC ACATGAAGGA TGCATGCATG 
CACAAACAAG TGTGACTCCT TTGGAAATAA GTATTCTTAA CGGTCACGGT 
GGGGACTAAA TTCCCACTTG AATATTCGTT TCGCGTCTGA AGCCCTGCAA 
CCTCCTACAA GATAGGTTGA AGTGAAACAA AGC
Go back to index
wmc(6e4) Plate reference : 6e4
WMC locus number : WMC Length of sequence : 375
Last updated : 14 Nov 1996 at 15:05
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR77. Trimmed to insert at 3'. (GT)9 337 to 354. No room for primers. Bases 28 to 62 show homology to 17g7 bases 27 to 61 (complement) (35 in longest run, maxScore = 45).
PCR Comments : 


Length of sequence : 375  Consensus sequence is :

..

GGCTTGTGCT GCCCATCGAC TCGCAAGTAA GGATGTGTGT GTTTGTCAAG 
GGTCCCCAGT TGAATGTCAA CCATCGACTC GCAANTAGGG GGTGCGCGTG 
TGTGTGTCTT GTCGTGGCCA CCAGTTGCAA GTCGCCCATC GACTCGCAAC 
TAAGGATGTG TGTGTGTTTG TGTCGAGGGT GCCCAGTTGT ATGTCGATAA 
CCGACTCGCA ATTAGGGGTG TGTGTGTCCT TTTGTCAGGG GCCCTAGTTG 
CATGTAAACC ATCGACTCGC AACTAGGGGG TGTGTGTTTG TTGAGGACCC 
CTGGTTGCAA GTTGACCATC GACTCACAAC TANGGGGTGT GTGTGTGTGT 
GTGTTTTGCT GTGGCCCCCA GTTGC
Go back to index
wmc(6f4) Plate reference : 6f4
WMC locus number : WMC Length of sequence : 337
Last updated : Lun 30 oct 2000 at 16:59
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR78. Trimmed to insert at 3'. (GA)26 159 to 210. Too difficult to design primers. bases 2-133 match part of 3a3 Bases 14 to 31 show homology to TW4c12.1 bases 64 to 81 (complement) (18 in longest run, maxScore = 40). Bases 86 to 142 show homology to TW6e01.3 bases 48 to 104 (57 in longest run, maxScore = 83).
PCR Comments :
Reverse primer details :
at length bases, Tm 


Length of sequence : 337  Consensus sequence is :

..

CGCCGAACGG ACGGCACCTC CGCGTTCAAC AGACGTACGG TTGGGAAGAC 
GTCTCCTCCT TCTTGATCCA GCAGGGGGAA GGAGGGGTTG ATGGAGATCC 
AGCAGCACGA CGGCGTGGTG GTGGAAGCAG CGGTGATCTC GGTTCGCCAA 
GCTCCAACGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 
GAGAGAGAGA GGTTATAAGG GGAGAGGGAA GGGCCAGGGA CTTGGTGCGG 
CTGCCCTCCC TCCCCCACTA TATATAGGGC CCCTAGGGGG GGCGATGGCC 
CTAGAGATTG GATCTCAAGG GGGGTGGCCA AGGGGGG
Go back to index
wmc(6g4) Plate reference : 6g4
WMC locus number : WMC Length of sequence : 308
Last updated : Jeu 27 Aož 1998 at 12:04
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR79. Trimmed to insert.(GA)10 79 to 98, (GT)12 55 to 78. Too difficult to design primers. Matches 8h9, first 50 bases are found towards end of 1C2. Bases 184 to 215 show homology to 14c4 bases 418 to 449 (complement) (32 in longest run, maxScore = 77). Bases 26 to 261 show homology to 10d8 bases 245 to 480 (236 in longest run, maxScore = 152), 6g4 has precedence.
PCR Comments : 


Length of sequence : 308  Consensus sequence is :

..

GTACAGATGC CGATACGTTG TGTATACATG AGAGAATGGT CTTCTAGTTT 
ACTTGTGTGT GTGTGTGTGT GTGTGTGTGA GAGAGAGAGA GAGAGAGAGG 
GAGGGAGGGA GAGAGAGGAG CATCCATAAC ACAACAACCA TCTCTCTCGA 
TTCGGCCATC CCTCGCACGA TCGCATGCAA CATATGCATC AACGCGCACA 
TTTTGACACC CTCACTCGGC CCCTACCCAC CTCAAGGCCC GCGCAATCTC 
TTCGTGAATA CCCATTTCTC TCCCTTAGGT TTGTTTTTGC TCAATATCTG 
ACACACAC
Go back to index
wmc(6h4) Plate reference : 6h4
WMC locus number : WMC Length of sequence : 330
Last updated : 14 Nov 1996 at 19:45
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR80. Trimmed to insert. (CA)8 39 to 54, (CT)8 135 to 150. Member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 9H3 . No primers designed to this.
PCR Comments : 


Length of sequence : 330  Consensus sequence is :

..

CCCCCCCACA CACACACTAA GAGTCCATCT CTCTCTCTCA CACACACACA 
CACAAACTAA GACTACATCT GTCTCTCTCT CCTCTCTCTC TCTCTCACAC 
ACACACACAA ACTAAGACTC CATCTGTCTC TCTCCTCTCT CTCTCTCTCT 
CACACACACA CACCACACAC ACACTAAGAC TCCATCACAC ACACATGCTC 
TAGGCGGAGC ATTTGTTCCT ACCACCCTTC ATCCAACCTT ACCCGTATGA 
TAAACAATCA CCTTTATTTA CTTGCATAAC ATGGAAAAAT TCCACTTTAC 
TTTAGTAGTC AGCAGACTTA TCATCTGTAC
Go back to index
wmc(6a5) Plate reference : 6a5
WMC locus number : WMC Length of sequence : 312
Last updated : 14 Nov 1996 at 15:10
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR81. Trimmed to fragment at 3'. (CA)10 36 to 55. Relative 6e3. No room for primers.
PCR Comments : 


Length of sequence : 312  Consensus sequence is :

..

ACGGCCAGTG AATTCCTCGA GGCACACACA CACACCACAC ACACACACAC 
ACACATTGTT TTTACTTATT CTCTATTTTC TGTTGTTTAG ACACAAATAT 
GATTTTTCAA ACCGTTATGG TTAGGCGTTG CCGCTCCTCC ACCAACACCA 
CGCCGTCGTG CTACTGCTGG ACAGAGTCTT CCCCAACCTC TCCCTCTCTC 
CTTGCTAGAT CAAGGCATGG GCGACGTCAC TGGGCTGCAC GTGTGTTGAA 
CGCGGAGGCA CCGTTGTTCG GCGCTTAGAT CAGAATCGAC CGCGATCTGA 
ATCGCCACGA GC
Go back to index
wmc(6b5) Plate reference : 6b5
WMC locus number : WMC Length of sequence : 0
Last updated : 14 Nov 1996 at 16:14
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR82. No insert.
PCR Comments : 


Length of sequence : 0  Consensus sequence is :

..

GAGGGATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTT
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wmc(6c5) Plate reference : 6c5
WMC locus number : WMC Length of sequence : 160
Last updated : 19 Nov 1996 at 12:21
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR83. Trimmed to insert. (CT)9 386 to 403, (CT)9 112 to 129. No room for primers
PCR Comments : 


Length of sequence : 160  Consensus sequence is :

..

CGCACACACA CAAACACATA CACACACACG CACCCACCCC CCACACACAC 
TAAGACTCCA TCACACACAC ACACTAAGAC TCCATCACAC ACACACACAC 
TAAGACTCCA TCTCTCTCTC TCTCTCTCTC ACACACACAC ACGCCATGCA 
CCCCTCCCCC
Go back to index
wmc(6d5) Plate reference : 6d5
WMC locus number : WMC Length of sequence : 389
Last updated : Wed, Aug 27, 1997 at 8:27 am
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR84. Trimmed to insert st 5' end. (GA)11 19 to 40. No room for primers. Matches 2e10, 13B1 , bases 1-252 match part of 4C11. Bases 258 to 301 show homology to 12h7 bases 93 to 136 (44 in longest run, maxScore = 114).
PCR Comments : 


Length of sequence : 389  Consensus sequence is :

..

GCGCGTGTGT GTGTGTGTGA GAGAGAGAGA GAGAGAGAGA TAGAGAGAGA 
GACCCATCGG CCGATGCTCG TAAATACATC TTCAGCCATA TGCATTGCTG 
ATGCTCAGTA AAAACTTATA TAGTTGACAC AATCATATAG AACATCATAG 
CACACTGAAC TTGCATACAA AAATTTCACG CAAGCGGCAC AATCAGGATG 
GAAGCAGTGG ATCGCTACGG GAAGGGCTAT GTTGAATCCA ACGAAGTCAT 
ACATAATGGT TCATCGTCTT TATCAATGAC GGGGAAATAT CTTGAATGTT 
GTGCAAAGAA AACAGACAGA CAACACAACA AGTTCTCCTA GCACATTAAC 
GCTTGACGTT CAGTCCACGT AGTCCACCGC GCCTCGAGG
Go back to index
wmc50(6e5) Plate reference : 6e5
WMC locus number : WMC50 Length of sequence : 539
Last updated : Tue, Aug 19, 1997 at 5:23 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR85. Trimmed to insert at 3' end. Multiple inserts (2) joined 219-236 by linkers. Microsatelilite in first insert too close to end to make primers. First insert matches 7B1. Second insert (GT)10 and (GT)16 repeats 402 - 456. Primers are to second insert.
PCR Comments : 61C anneal, product;
Forward primer details :
cTgccgTcAggccAggcTcAcA at 349 length 22 bases, Tm 76
Reverse primer details :
cAAccAgcTAgcTgccgccgAA at 472 length 22 bases, Tm 74


Length of sequence : 539  Consensus sequence is :

..

CATACACACA CACACACACA CGGACACACA CAGACAAACA CACACACACA 
CATATGGTTT TTACTTATTT TCTTTTTCCG GTTTGTTTAG ATTAATGTTA 
GATAAATTAC ATAGATAAGA ATGTTAGAAT GTTAATGTTA GATATTAATG 
TCAATTTTAG ATGAATTCGC TAGATATTAA TTAGGTATAT ATATGAGATC 
TGAGCTAGTT GAATTAATTA GTCCACGCGT GGACTACGTG GACTAAGTAT 
TTATGACCAC CTTTCGAAGT GAAAGGAGCC GGACCCCATA CATCAGAATG 
GACTAAATCG AAAGGACGCT GAGACACTGA CACCACCGCA TGCAGCAGCT 
GCCGTCAGGC CAGGCTCACA TGATCGGATC GCCCGTCCGC GAGATCTGAT 
CCGTGTGTGT GTGTGTGTGT GTGCGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGGAG CTGATCAGGA ATTCGGCGGC AGCTAGCTGG TTGGTTGGTT 
GATCGATCGA GGGGAGAGAA GAGAGGATCG AGATGATGC
Go back to index
wmc(6f5) Plate reference : 6f5
WMC locus number : WMC Length of sequence : 968
Last updated : Tue, Aug 26, 1997 at 12:11 pm
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR86.Trimmed to insert. No overlap between forward and reverse sequences. (CA)18 855 to 890, (CA)9 913 to 930, (CT)9 941 to 958. No room for primers. Bases 164-296 match 8f10 complement. Bases 725-928 match part of 9d4. Bases 161-327 match part of complement of 11H12. Bases 1-32 match (most of) 5h2. Bases 4-171 match 4E6 (complement).
PCR Comments : 


Length of sequence : 968  Consensus sequence is :

..

GTACCTCTCA CACATGCACG TTGAAGGTCT CTATGTATCC ATACGTAGTT 
AATTACCCTC AACCCTAACG CCACATCGAA TCGTTCTCTT CTTGTGTGCA 
CATAACTTCC GCCTGGATGG GTAAAACACA CACTCGCACT TAACAATCTC 
CTTCTAGCTA CCCCCCTCTC ACTCTTCCCC TATATCCCCA AAACCAATAA 
GACCATCCCT TCGTTTAATT CCCGCCAACA TGCACCATCG ATATATAGTA 
GTCTTCCTCG GTGTCTCTCG AACAAACATG TTCTCTCGAT GTCGACTACT 
CTCACGCGCA CACACCTTCT CTTCCCTGGT GATGCTGCCA ACTTACTGAT 
TTAGTGTATG ATGGTGTTTT TGAGGTGCTC CAGTGGCTTC TGTTTCTATC 
AGCTGTCCCT CCTGTTCAGC TACTGACGGG GTGGTGCGTA ACGGCAAAA
(break)
CTGGGGCGTC TTATGAGCCT GCTGTCACCC TTTGACGTGG TGATATGGAT 
GACGGATGGC TGGCCGCTGT ATGAATCCCG CCTGAAGGGA AAGCTGCACG 
TAATCAGCAA GCGATATACG CAGCGAATTG AGCGGCATAA CCTGAATCTG 
AGGCAGCACC TGGCACGGCT GGGACGGAAG TCGCTGTCGT TCTCAAAATC 
GGTGGAGCTG CATGACAAAG TCATCGGGCA TTATCTGAAC ATAAAACACT 
ATCAATAAGT TGGAGTCATT ACCCTCTTCC CTCTCTACGG GCATTTTCTC 
TCTCGCACCC CATCGTTGGT GGCCTCTGCC ATACACATCA CCCCTCTATG 
ATGGAGCCAT GCACACTTAA ATTAGATCCG CCACCGAGGA CCCCGCGACA 
CACACACACA CACACACACA CACACACACA CACACTAAGA CTCCATCACA 
CACTCGCACA CACACACACA CACAAAGACT CCATCTCTCT CTCTCTCTCT 
CTCACACACA CACACACCC
Go back to index
wmc51(6g5) Plate reference : 6g5
WMC locus number : WMC51 Length of sequence : 359
Last updated : Thu, Jun 11, 1998 at 11:28 am
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR87. Trimmed to insert. Interrupted (GT)12 microsatellite 259-272. (3 loci?).
PCR Comments : 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
TTATcTTggTgTcTcATgTcAg at 230 length 22 bases, Tm 57
Reverse primer details :
TcgcAAgATcATcAgAAcAgTA at 297 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 89 bases.


Length of sequence : 359  Consensus sequence is :

..

GTACTTCTGG ATTCAGGCAT TCGCAACTGT GAACTCGAGT TTAACAACCG 
GTATATCACT GCTGGATGGT GAACACACAC AATGGTGCTT TTATTGATAG 
AAAGTCAGGA TTCTTTCTCT TAAGAGGCTA CTGTAAGAAA TGGTAACTTG 
GGGCATGACT TGCAAGAAAG GGATTGTATG ACAGAATGAT ATTACTTCAC 
GGTAGAGTTT CTGCATCTGG AGATCAAGTT TATCTTGGTG TCTCATGTCA 
GTTTTATTGT GTGTGTGTGC GTGTGTGTGT GTTTCAGTAA CACATTTACT 
GTTCTGATGA TCTTGCGAAT TTGTGAGCCG AAGAAATGAT GACTTAGATA 
GTTGTGTTC
Go back to index
wmc52(6h5) Plate reference : 6h5
WMC locus number : WMC52 Length of sequence : 608
Last updated : Thu, Jun 11, 1998 at 11:28 am
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR88. Trimmed to Rsa1 fragment. (CA)20 420 to 459. relative 6g6. Penner maps to 1B, Jorge to 4D. We detect 2 loci.
PCR Comments : 61C anneal, product;
Forward primer details :
TccAATcAATcAgggAggAgTA at 322 length 22 bases, Tm 62
Reverse primer details :
gAAcgcATcAAggcATgAAgTA at 492 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 192 bases.


Length of sequence : 608  Consensus sequence is :

..

GTACGATTAT ACAACTTAAC CAATCGCGCG GATAATCACG AGACATTTCA 
GTTGTGCACA AAGTCATAAA CGTCTTGTCA ACCTTATGTG AAGACTGCCT 
CTATACATAC ATAGTATATT AACAACTCCT TTGCAATTAT TTCATTTGCT 
TGAGGAAGTA AGTGAGAAAC CTCGGGTTTC CTGGATTTAA TTAGCTTGGC 
GCTGTCCCCT GTTAACGAGC CAACATCCAT GCCTTGTCAA ACCATACCTA 
AACCAATAAT ATCCATTTCC ACGCAGCAAT GGATAGCAAA CTTATTTAAT 
TCCTCAGATC ATCGCTAAGT ATCCAATCAA TCAGGGAGGA GTAGCAAATA 
TGTATGTATG TTAGTTCACC TGACATTGAT GGGGTGGATA TTGAGATTCT 
TCCAACTCCT ACACACACCC ACACACACAC ACACACACAC ACACACACAC 
ACACACACAT GATTTACATG TCCATGAACT GAAATTCACA ATACTTCATG 
CCTTGATGCG TTCATCTACG TGCTACAAAA AAGCAACAAG AAGAAAAGGC 
ATTCTAGACA CTTGGTCTTG TTGCGGCTTG ACCGGGTTCA AAAGTATCGG 
GTAGGTAC
Go back to index
wmc(6a6) Plate reference : 6a6
WMC locus number : WMC Length of sequence : 368
Last updated : 6 DŽc 1996 at 9:42
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR89. Trimmed to fragment (CA)8 33 to 48. No room for primers. Same as 2c8, 6B6, 7a2, part 11H2.
PCR Comments : 


Length of sequence : 368  Consensus sequence is :

..

TACACACACA CACACAGACA CACACGCACG TGCACACACA CACACACACG 
CAACACAACA CACACACACG CACTCCCTCA AGCACACAAT TCATCCCCAT 
CCAAGGTTAT ACGGGGACCA CTCTCGCTTG TGCTTCTTTG CACCCCAACA 
TGCACAACAC CTCAATCTTC AAAGAGGGCA TCGAGCAGAT CGAAGACGAC 
GACCACACCG CGCTAGAGAA TGCAGGGTCA TTTGGAAGCA AGGTGATGTG 
ACGACGGCTG ACTGACTCCT CCCCCACTCT CTCTCTCTCT TGCACAAAAT 
TACCAATCCC TCTCTCCCCC ACACAAAATT GTCCATCCCT CAACCCATGA 
GATCCTTCCC CTCTCGTC
Go back to index
wmc(6b6) Plate reference : 6b6
WMC locus number : WMC Length of sequence : 362
Last updated : 15 Nov 1996 at 9:44
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR90. Trimmed to fragment at 3' end. (CA)8 27 to 42. No room for primers. Same as 2c8, 6a6, 7a2
PCR Comments : 


Length of sequence : 362  Consensus sequence is :

..

CACACACACA GACACACACG CACGTGCACA CACACACACA CACGCAACAC 
AACACACACA CACGCACTCC CTCAAGCACA CAATTCATCC CCATCCAAGG 
TTATACGGGG ACCACTCTCG CTTGTGCTTC TTTGCACCCC AACATGCACA 
ACACCTCAAT CTTCAAAGAG GGCATCGAGC AGATCGAAGA CGACGACCAC 
ACCGCGCTAG AGAATGCAGG GTCATTTGGA AGCAAGGTGA TGTGACGACG 
GCTGACTGAC TCCTCCCCCA CTCTCTCTCT CTCTTGCACA AAATTACCAA 
TCCCTCTCTC CCCCACACAA AATTGTCCAT CCCTCAACCC ATGAGATCCT 
TCCCCTCTCG TC
Go back to index
wmc53(6c6) Plate reference : 6c6
WMC locus number : WMC53 Length of sequence : 274
Last updated : Lun 2 Nov 1998 at 9:58
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR91. Trimmed to Rsa1 at 3' end. (CA)20 67 to 106. Matches 9d1 with a few differences (6c6 carries and extra 5 CA repeats), after base 80 strong match with 1D11 (start of first microsatellite). 5' side of microsatellite matches 7b7 with some mismatches. Bases 50 to 100 show homology to 23b5 bases 144 to 194 (complement) (51 in longest run, maxScore = 133), duplicate, 6c6 has priority.
PCR Comments : 51C anneal, product;
Forward primer details :
cTgTAggAgTAggTcgATcTgT at 15 length 22 bases, Tm 57
Reverse primer details :
AcTcccTccgTcTAggTgTAAT at 155 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 162 bases.


Length of sequence : 274  Consensus sequence is :

..

TACACAACAT CTCTCTGTAG GAGTAGGTCG ATCTGTCGCT CTCTCTAACA 
CACACATGGT GTTAAACACA CACACACACA CACACACACA CACACACACA 
CACACATACA CACATACACA CACCTTGGTC CCGTTGCACC TTCTAACATG 
ACTTATTACA CCTAGACGGA GGGAGTAGTA AGTATACAAG ATACGGTGGC 
ACACTGACTT AAGTCGAATA CAAGTGCCCA AAATTTGTGT GCATGTTATA 
ATAACGATTC ACTTAAAATA GTAC
Go back to index
wmc(6d6) Plate reference : 6d6
WMC locus number : WMC Length of sequence : 246
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR92. Trimmed to fragment. Interspersed CT and AT microsatellite 5-55. No room for primers. Bases 48 to 89 show homology to 19e1 bases 1 to 42 (complement) (42 in longest run, maxScore = 26). Bases 74 to 104 show homology to 25d4 bases 108 to 138 (31 in longest run, maxScore = 24).
PCR Comments : 


Length of sequence : 246  Consensus sequence is :

..

GTACCACACA CATACATACA CACACACACA CTACACACAC ACACACTACA 
CACATTAGTT TGTTTGTTAT AAATTTTTCT GTTTTTTAGT TATAGAAATG 
TTAGAAATTA TTCTGTGGAA TGACGGAAGG TAGGTGGTTT CAAACCAATG 
AAATCATAGA TGGTCGCTGA CTCTTTGCAC TGAGGTGCGG TGTTCTCCTC 
GATACGTGCT AACAAGCGGT TAGACTCATG CTTGCTCCTC TCCATC
Go back to index
wmc(6e6) Plate reference : 6e6
WMC locus number : WMC Length of sequence : 543
Last updated : 15 Nov 1996 at 9:47
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR93(CA)8 70 to 85, no room for primers
PCR Comments : 


Length of sequence : 543  Consensus sequence is :

..

AACGGTCAAA TCGGGGGCGT CACCCCCCCT TCGCACACAC ACCCCAAAAC 
CTCCTCGGGC GCGCGCGTGC ACACACACAC ACACACTCTC ACACACACAC 
ACACCAACAC CACCACCACC ACAACAACAA CAACAACCCC TCCCTCGCTC 
GAAACCCTAG CAAGGTCGCT ACCGCCACCG CCGCAAGGCA TCCATTTTCA 
ATATCTACTG GTTTATCCAT GCAGCTTACC CACAGATCTC CATACCCAGG 
CCGCCCTGAG TTTCTTAGAT GACGACTGCC AAATGCCCCC TTTCCACAGA 
TCCCCATCCC CATCCCCCAC TCTAGAGCAT CGCAGCCTAA GCCCGGCTAC 
GCTACCCGTG GAGCACGAGG AGCGACTGGC CCAAAAGAGG TGTATTGCCG 
CCTCTTTGCT TGACGTCGCC GAGGTGGCCA CCGAGGAAGA TGATGACCAT 
TACTGGCCGC AGGCACTCAA TCGGCGGGCT AGAGTATGTG GGTATGTCGC 
GGACGCCAGC TACGACATCA AGGTCTCCTC CACGCGCCTC GAG
Go back to index
wmc(6f6) Plate reference : 6f6
WMC locus number : WMC Length of sequence : 150
Last updated : 15 Nov 1996 at 9:49
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR94. Trimmed to insert. No substantial microsatellite.
PCR Comments : 


Length of sequence : 150  Consensus sequence is :

..

GTACTGCTCC GTGGCGGGAG GCATGGCGAA AACGAACGCG GCGGCGGAGG 
AACGAGTGGT GGAAGAGCGC GGCGGCGAGG CGCTATTGCA AGAAAGGGCA 
GGAGGAAGAA GATGGTGGAA GGAGGAGGAG CGTGCGAGAG CCTGCCGCTC
Go back to index
wmc(6g6) Plate reference : 6g6
WMC locus number : WMC Length of sequence : 591
Last updated : 4 DŽc 1996 at 17:53
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR95. Trimmed to Rsa1 at 3' end. (CA)21 401 to 442. Relative 6H5 (primers shown on 6H5).
PCR Comments :
Expected size of amplified fragment (Chinese Spring=) 262 bases.


Length of sequence : 591  Consensus sequence is :

..

CCAATCGCGC GGATAATCAC GAGACATTTC AGTTGTGCAC AAAGTCATAA 
ACGTCTTGTC AACCTTATGT GAAGACTGCC TCTATACATA CATAGTATAT 
TAACAACTCC TTTGCAATTA TTTCATTTGC TTGAGGAAGT AAGTGAGAAA 
CCTCGGGTTC CCTGGATTTA ATTAGCTTGG CGCTGTCCCC TGTTAACGAG 
CCAACATCCA TGCCTTGTCA AACCATACCT AAACCAATAA TATCCATTTC 
CACGCAGCAA TGGATAGCAA ACTTATTTAA TTCCTCAGAT CATCGCTAAG 
TATCCAATCA ATCAGGGAGG AGTAGCAAAT ATGTATGTAT GTTAGTTCAC 
CTGACATTGA TGGGGTGGAT ATTGAGATTC TTCCAACTCC TACACACACC 
CACACACACA CACACACACA CACACACACA CACACACACA CATGATTTAC 
ATGTCCATGA ACTGAAATTC ACAATACTTC ATGCCTTGAT GCGTTCATTT 
ACGTGCTACC AAAAAGCAAC AAGAAGGAAA GGCATTCTAG ACACTTGGTC 
TTGTTGCGGC TTGACCGGGT TCAAAAGTAT CGGGTAGGTAC
Go back to index
wmc(6h6) Plate reference : 6h6
WMC locus number : WMC Length of sequence : 624
Last updated : Lun 5 juil 1999 at 17:23
Owner : Dubcovsky, Buenos Aires Date at Agrogene : 9.9.96 Public Domain : Yes
Comments : AGR96. Trimmed to Rsa1 at 3' end. No substantial microsatellite. Bases 582 to 590 show homology to 2f6 bases 44 to 52 (complement) (9 in longest run, maxScore = 19). Bases 517 to 538 show homology to 26e11 bases 135 to 156 (complement) (22 in longest run, maxScore = 19).
PCR Comments : 


Length of sequence : 624  Consensus sequence is :

..

GGATAACACG CGCGCTCCTC CTTCTCCGCC GCCCGCCTCG CCTCGCCTCA 
CCTCGCCTCG TCATGAGCCG GGCCGAAGCT TATTTTTGCC GCTCAGGAAT 
GGTTAATTAA TTAACGAGTC GCTTACAGAA GCGCCACTGT CCGAGACGTT 
GGACCGTGGG CTGTTCGCAG ACTCGGTCGT GGGCCTGGCC CAGGCCCATC 
TACCTGACGG CCTATATAAG AAGGCGCTGG CCACTGGAGT GAACCCTAAC 
TCAGTTCACT CACTCCCTCT CGCACAACCC TAGCCGTCAT CTACTGTTCC 
TCTCACTGTG CTGCCTCCGG CGATCCCACC CCGACGGCCG CGTGCACGGT 
TGGTCGGGAG AGCAGGTGCC TCCGGAACCC TGTCGTTCGA GATCCTGCCC 
GGGAGAACGG CAATAAGGTT TTTGGGGAGC GTCTCGACGC GACTGCTCCC 
GACCCGTCCC CGTCTCCGTC CGCCCCTGCT TCCTTTACAT CGACCCCATG 
GCTAACAACG TCGCCACCAA GAAGAAGGCC ACGGACGACA CCGAGGCTGC 
CGCCGCCGCC GCCGCGTTGG CCTGGCCAAC CGGAGGGTAT GATCTGTTCA 
TCCCTCGTTT ACTCGTACTT GTAC
Go back to index
wmc(1A7) Plate reference : 1A7
WMC locus number : WMC Length of sequence : 287
Last updated : 15 Nov 1996 at 15:25
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : Trimmed off vector sequence that was causing crossmatch. No substantial microsatellite.
PCR Comments : 


Length of sequence : 287  Consensus sequence is :

..

ATCATATAGG GCCATTCTAA TCGGTACCGG GGATCCTCTa CAGTCGACCT 
GCAGGCATGC AAGCTTGAGT TTCTaTCATG TTCTAGCGCA GTGTGGTCAT 
CGTCTTCGAT CTCCTCGATG CCCTCTTTGA AGATTGAGGT TTGTGCATGC 
TGGGGTGCAG AGAAgCACAA GCGTGAGTGG TCGCCATATA ACCTTGGATG 
GGGATGACCG AGAACGGAGG AAACGGCCTT GTGTTCTACC GGTCACGGTC 
AAAACGGTAT CCTGTTCATC GGGAGGGGTC GGCGTAC
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wmc(1b7) Plate reference : 1b7
WMC locus number : WMC Length of sequence : 225
Last updated : 26 Sep 1996 at 8:59
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments : 


Length of sequence : 225  Consensus sequence is :

..

ACTAGCCCCA GCCCGACCAG GCAGTTCTTC ATCTCTCCGC TCTCTGGACA 
GAGAGCGGCG GCGGCGGCGG GCCCCCATCT AATCCCCCCA CATCCCTCTC 
AGATCTGAAG ATTTGATCCG TGGATTCGAT CCCCAATCCA CCTACTAGGT 
AAACTCTTCC CTTCTCTTTC TTTTGCTCCG TAAATTTGTT GCCATTCTAG 
AGATTTGTCC TGATTTGATG GAACC
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wmc54(1c7) Plate reference : 1c7
WMC locus number : WMC54 Length of sequence : 315
Last updated : Fri, Jun 20, 1997 at 4:28 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (GT)8 176 to 191, (GT)8 194 to 209. Bands tend to be faint.
PCR Comments : tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Forward primer details :
TATTgTgcAATcgcAgcATcTc at 141 length 22 bases, Tm 65
Reverse primer details :
TgcgAcATTggcAAccAcTTcT at 261 length 22 bases, Tm 69
Expected size of amplified fragment (Chinese Spring=) 142 bases.


Length of sequence : 315  Consensus sequence is :

..

ACTAAGGCAC TATAGGAGCT TTGTCAAGCT TCGGGGCTGG AACACCAAGC 
TTGATCATAC ATTGATTTGT TTGATTTTGA GAAAACTCCA CTGGAGCACC 
TACAAGAAGG TGGTCGCATT ATCATCCTGT AAACCAAAGT TATTGTGCAA 
TCGCAGCATC TCAAGGGATG TGATTGTGTG TGTGTGTGTG TTTGTGTGTG 
TGTGTGTGTG GTGTGTGTGT GTAGAAAGGC TACTCGATGA CCTCACAAAT 
AAGGAATACA AGAAGTGGTT GCCAATGTCG CAAAAAACTT ACTATTTCCT 
ATGTAAGAAT CTAGT
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wmc(1d7) Plate reference : 1d7
WMC locus number : WMC Length of sequence : 392
Last updated : Mon, Aug 25, 1997 at 5:27 pm
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : No substantial microsatellite. Vector sequence found at 3' end trimmed off 5.8.97. Duplicate of 4F2.
PCR Comments : 


Length of sequence : 392  Consensus sequence is :

..

ACTAACaGGG CCCAGTTGCC GgcGGACAGC AAGATcCACC CAGTCTTCCA 
CGTATCCCAG CTCAAGCCAT CACCCCaACT ATACTCCGGT CTTCTCCGAG 
CTGCCACGAC CCCCAGACCT CGCCGGCCAC GACGACGAAC GGTGGAGATC 
CTGGAGCGGC GCATGGTCAA GCGCGACAAC AGTGCTCTGG TGCAGGTCAA 
GGTGCGCTGG GCAACGATGA GACGGCGACG ACTTGGGAGG ACTACGACAC 
CCTTCGTCGT CGATTTCCGC AAGCACCGGC TGGAACGCAC CACCAGCCGG 
CGACGCGGAG CAGCACGCGA AACCTGAAGA CGGCgCacgT TCTgAAGGAG 
GGGGAATGTC ACACCTGCAT CACCAGGAGA CGATGCGGTG GA
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wmc55(1e7) Plate reference : 1e7
WMC locus number : WMC55 Length of sequence : 217
Last updated : Ven 12 FŽv 1999 at 15:33
Owner : Waugh, SCRI Date at Agrogene : 12.9.96 Public Domain : Yes
Comments : (CT)9 92 to 109, expected fragment 199 bases. Bases 48 to 140 show homology to 26c3 bases 45 to 137 (complement) (93 in longest run, maxScore = 43). Duplicate with 26c3, 1e7 has priority.
PCR Comments : 61C anneal, smear;
Forward primer details :
AcAcATcAccTcTcTATgATgAAgc at 4 length 25 bases, Tm 62
Reverse primer details :
ggAATTTgTccATgTTATgcAAgTA at 178 length 25 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 199 bases.


Length of sequence : 217  Consensus sequence is :

..

ACTACACATC ACCTCTCTAT GATGAAGCCA TGCACAATTA CACACACGCA 
CGCACGCACA GCCCCCCCCC ACACACACAC TAAGACTCCA TCTCTCTCTC 
TCTCTCTCTC ACACACAAAC ACACACACAC CCCACACACA ACTAAGACTC 
CATCTCACAC ACACATGCAC CTTTATTTAC TTGCATAACA TGGACAAATT 
CCACTTTACT TAGTAGT
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