Wheat microsatellite WMC 25/06/01
wmc438(24f5)
Plate reference : 24f5
WMC locus number : WMC438 Length of sequence : 318
Last updated : Ven 13 Nov 1998 at 14:19
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)17 106 to 139, (CA)9 142 to 159.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
gAccgTTgggcTgTATAgcATT at 8 length 22 bases, Tm 64
Reverse primer details :
cTcTgAcAgTggTggAgcTTgA at 242 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 256 bases.
Length of sequence : 318 Consensus sequence is :
..
CCTCTCTGAC CGTTGGGCTG TATAGCATTT ATCGACTCTT CAGAGACCTG
CAGCGACGGG AGCTTCACGA GGACCAACCA CCTAGGCCCT CCCACCTTGG
CTAAACACAC ACACACACAC ACACACACAC ACACACACAC GCACACACAC
ACACACACAA GTAAGGGTAT GTTTAGCCAT TAATGACGCA GTTTTGCCCG
AACTTTGCCC ATTCTACACT TCTATAAGAA AAAAAAACTG ATCAAGCTCC
ACCACTGTCA GAGAGTGAGG TTCACGGCAC GTGCAGCTCG ATTATAGGAG
TACTAACTGG ACTGGAGC
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wmc(24g5)
Plate reference : 24g5
WMC locus number : WMC Length of sequence : 205
Last updated : Jeu 29 Oct 1998 at 16:51
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends ( NB 3' linker corrupted). No substantial microsatellite.
PCR Comments :
Length of sequence : 205 Consensus sequence is :
..
CGCACTGTCG TTGGAGCCCA CGTCTACATC CGCATTATGT CAAAATTGCT
TAACGGATGT GCGCAAATCG CCGCCTCAGC GGTCCGGTCT ACATCAACTC
ATCGGGACTG TGCCTACAGT CGATTTATTC GACCGTGCCC GCGCCGACCT
AACCGTCCGC ACCCACGATC AACTTGTCTG TCCGCACAGC TTACAAATAC
CGCGG
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wmc(24h5)
Plate reference : 24h5
WMC locus number : WMC Length of sequence : 215
Last updated : Lun 2 Nov 1998 at 10:06
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 21 to 148 (complement) show homology to 10f10 bases 90 to 217 (128 in longest run, maxScore = 153). No substantial microsatellite.
PCR Comments :
Length of sequence : 215 Consensus sequence is :
..
GATTTCGTTC AGTATGACTT CGACTTTAAA TTATACGACG ATCGATCTAA
AATAAACGGA AATGATAAAC GTTCGGATTT TAAGCATGGC AATCCGAATG
TTTTTCATGG CAAATTTAGA TTACGCGGCG GCGGCGGGGG CGTCTTGCAG
TGGGAAGCGG CTCCTCTGTC GTGCTCTGTG TGTCGTCGGG CCACTGACCG
ACGGTGACGG TGGCG
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wmc439(24a6)
Plate reference : 24a6
WMC locus number : WMC439 Length of sequence : 128
Last updated : Ven 13 Nov 1998 at 17:38
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 56 to 83.
PCR Comments : 51C anneal, product.
Forward primer details :
AATgTAgATcTcTcTTTcTgTg at 19 length 22 bases, Tm 52
Reverse primer details :
AgTgTcTAcTATAcccTgTgcT at 84 length 22 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 87 bases.
Length of sequence : 128 Consensus sequence is :
..
CTCATCTATT GTTCAGTAAA TGTAGATCTC TCTTTCTGTG TGTGTGGGGG
TGTGGGTGTG TGTGTGTGTG TGTGTGTGTG TGTAGCACAG GGTATAGTAG
ACACTGTATA TTTTTGCGTG ACAATATT
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wmc(24b6)
Plate reference : 24b6
WMC locus number : WMC Length of sequence : 175
Last updated : Ven 13 Nov 1998 at 14:22
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 175 show homology to 24a5 bases 1 to 175 (175 in longest run, maxScore = 66). (TTG)10 25 to 54, (TTG)6 58 to 75, (TTG)7 79 to 99, (TTG)6 145 to 162. Duplicate, 24a5 has precedence.
PCR Comments :
Length of sequence : 175 Consensus sequence is :
..
CTGCTGCTGT TGGTGTTGGT GGTGTTGTTG TTGTTGTTGT TGTTGTTGTT
GTTGTTCTTG TTGTTGTTGT TGTTGTTCTT GTTGTTGTTG TTGTTGTTGC
TGTTGCTATT GCTGTTGTTG TTGCTGTTGC TGTTGCTGTT GCTGTTGTTG
TTGTTGTTGT TGCTGCTGCT GCTGC
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wmc440(24c6)
Plate reference : 24c6
WMC locus number : WMC440 Length of sequence : 116
Last updated : Ven 13 Nov 1998 at 17:43
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' end corrupted). (GT)12 74 to 97.
PCR Comments : 61C anneal, product.
Forward primer details :
ggAAAccAAAAAgTcccgA at 13 length 19 bases, Tm 62
Reverse primer details :
ggTgggAggggAcTAgcAT at 98 length 19 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 104 bases.
Length of sequence : 116 Consensus sequence is :
..
GGCACGTGCC TCGGAAACCA AAAAGTCCCG ACACTAAGTG AGGTGTACTT
GTTCACGGAC GCGCACGCAT AATGTGTGTG TGTGTGTGTG TGTGTGTATG
CTAGTCCCCT CCCACC
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wmc441(24d6)
Plate reference : 24d6
WMC locus number : WMC441 Length of sequence : 164
Last updated : Lun 16 Nov 1998 at 9:20
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 144 to 164 show homology to 17h7 bases 131 to 151 (21 in longest run, maxScore = 38). (CA)10 53 to 72, (CG)9 73 to 90, (CT)9 35 to 52.
PCR Comments : 51C anneal, polymorphic on acrylamide (complex).
Forward primer details :
TccAgTAgAgcAccTTTcATT at 5 length 21 bases, Tm 58
Reverse primer details :
ATcAcgAAgATAAAcAAAcgg at 142 length 21 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 158 bases.
Length of sequence : 164 Consensus sequence is :
..
GGGTTCCAGT AGAGCACCTT TCATTTTTAG ATTTCTCTCT CTCTCTCTCT
CTCACACACA CACACACACA CACGCGCGCG CGCGCGCGCG TGCGCGTACA
CATTGTTTCT TGTTCCATTT TTTCCGGCAC TTGCATGCAC ACCGTTTGTT
TATCTTCGTG ATGC
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wmc(24e6)
Plate reference : 24e6
WMC locus number : WMC Length of sequence : 358
Last updated : Mar 22 D残 1998 at 9:42
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Bases 230 to 306 show homology to 5e3 bases 259 to 335 (77 in longest run, maxScore = 177). (GT)9 173 to 190. Duplicate, 5e3 has precedence. Bases 230 to 291 show homology to 25d7.1 bases 277 to 338 (complement) (62 in longest run, maxScore = 105).
PCR Comments :
Length of sequence : 358 Consensus sequence is :
..
CACCCTGCTT CCAAATGACC CCGCATTCTC CAGCATGGTG TGGTCGCCGT
CTTCGATCTG CTTGATCCCC TCTTTGAAGA TTGAGGTGTT GTGCATGTTG
GGGTGCAAAG AAGCACAAGC GAGAGTGGTC GCTGTATAAC CTTGGATGGG
GATGAATTGT GTGCTCGGGG GAGTGTGTGT GTGTGTGTGT TGTGCTGCGT
GTGTCTCTGT GTGTGTATGT GTGTGTGTTA GATATTGAGA GAAAACAAAC
CTAAGGAGAG AAATGGGTAT TCACGAAGAG ATTGTGCGGG CCTTGAGGTG
GGCAGGGGCC GGGTAAGGGT GTCAAAATGT GCGCGTTGAT GCATGTGTTG
CATGCGAC
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wmc(24f6)
Plate reference : 24f6
WMC locus number : WMC Length of sequence : 344
Last updated : Lun 2 Nov 1998 at 10:06
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 260 to 302 show homology to 14a3 bases 252 to 294 (43 in longest run, maxScore = 99). No substantial microsatellite.
PCR Comments :
Length of sequence : 344 Consensus sequence is :
..
CCCAAGTCTC ACAACACAGT ACAAAGGTTC TGCTGAGGGC ACAGCTCAAC
AAGCCCAAAA ATAAAAACCA GAAAAAGCCG GACGAGAAAG CGTCTAGTCT
GGTTCCGGCG GGGGCGGCGG CGATAGACGG ACAACCATCG AGCGGAGGCC
GGCGATGAAG GCGGAGATGG CGTCGCGGTC CTGAGGGCGG CTAAGCGGCT
GCCAAAGCTG CAAGAAACCC GAAAGTTTGA AGAGAGCGTC AGTAGCCCGT
CGAAGAGGAG TGCGCTGGAT CACAAGCTTA TTGCGGATCG TCCAGAGGGT
CCAAGCGAGG ACCCCAATCT CAAGCCACCT AATGTGGCGA GACG
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wmc442(24g6)
Plate reference : 24g6
WMC locus number : WMC442 Length of sequence : 286
Last updated : Lun 16 Nov 1998 at 9:32
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 256 to 271 (complement) show homology to 23A1 bases 32 to 47 (16 in longest run, maxScore = 19), homology on one side of microsatellite only. (CA)7 194 to 207, (CA)7 210 to 223, (CA)5 226 to 235, (AT)5 241 to 250.
PCR Comments : 51C anneal, polymorphic on acrylamide.
Forward primer details :
AcTAcTTTTTgcTTgAcAAcgA at 142 length 22 bases, Tm 58
Reverse primer details :
AgTAAcAAAATAgTccAgcccT at 251 length 21 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 131 bases.
Length of sequence : 286 Consensus sequence is :
..
GTTCACGGTT ATTGAGGCAT TTGTGGCACA TCAAAGTTGT GCATTTTATG
TATTATATTC AACATATCTT TTGGCCCACT TGCAAAGCGA TGGTGTTCAG
CGATATCGAT GCTTTCCATT TGTGGGCCCG CTTGGTCCAT CACTACTTTT
TGCTTGACAA CGAGAGTGGT TAATTTGACT TAGGAGGGGT ATACACACAC
ACACACAAAC ACACACACAC ACAAACACAC ACACAGAGAG ATATATATAT
AGGGCTGGAC TATTTTGTTA CTAGTAACAG AATATT
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wmc(24h6)
Plate reference : 24h6
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:51
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : No sequence 29.10.98.
PCR Comments :
No sequence available for this clone
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wmc443(24a7)
Plate reference : 24a7
WMC locus number : WMC443 Length of sequence : 229
Last updated : Lun 16 Nov 1998 at 9:38
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (CT)44 62 to 149.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
ccTccTcTgTTTTcccTcTgTT at 14 length 22 bases, Tm 63
Reverse primer details :
cAcAcTcTgTgcTTcTgTTTgc at 198 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 206 bases.
Length of sequence : 229 Consensus sequence is :
..
GCCGCGGCCT CAGCCTCCTC TGTTTTCCCT CTGTTTCTCT CTGTGTGTGG
GCCACATTTT TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC
TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC TCTCTCTCTC
GCTGCTTTGT GTTGGTGAGA GGGAGGGAGG GGTGCTTGGC GCCATACGCA
AACAGAAGCA CAGAGTGTGC CCGAATATT
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wmc(24b7)
Plate reference : 24b7
WMC locus number : WMC Length of sequence : 568
Last updated : Jeu 29 Oct 1998 at 16:51
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :
Length of sequence : 568 Consensus sequence is :
..
CAGAGTCCCT TACCTTTTTG CACCCAAGAA ATGGCGCGGC GGCGGGCGGC
GGAAGCAGCA GTATCTGATG GCTGCATGCC ACTGGGCGGC CCTCCCTTCA
TGCCTTGCAT CCAGCCGAGG CTCCGCCGGG TGCGTGCGAG AGGCGGCGGA
ATGGTGTGGC CATGGACAAG ATCGCCGGCC ACAAGGACGA GCAGAAGCAG
AGCCATGAGC GCTGTTTTGG GTGCCATTCT TCTTGCCGTC TATGCTCCTC
TTTTGCTAGC TGCTGGATCT GCCCCAAACA CCAGTAGTCT GCACCTGCAC
TGCACATGGC CGGGGAGTGC AGATATATAG CTCAAGTGTG GATCGGTGCA
TGATTACTGC TACTACTCTG CAGACTCGTA GTCGTAGGTT AGGCCTTTGA
TGAGACACGA TGAATGCGTA GCAAATGAAT GGAGGAATCC GTATCTTGTA
GCGCCCCAAA GGAGGTTGAC AGGTCAGCCC CAACATCACA GACACGCACA
CACGCTAACA AATCACATGG AATTTGCAAC AGCTTGTACA CTCTTGTTGG
ATCTAGTGAT CAGCTGGA
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wmc444(24c7)
Plate reference : 24c7
WMC locus number : WMC444 Length of sequence : 349
Last updated : Lun 16 Nov 1998 at 10:05
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 66 to 80 show homology to 22a11 bases 153 to 167 (15 in longest run, maxScore = 46) Homology on one side of microsatellite. (GT)10 225 to 244, (GT)9 247 to 264, (CT)9 207 to 224. Reverse primer is outside region of homology.
PCR Comments : 61C anneal, product.
Forward primer details :
gTTTcTTgTAAAccAcgccTTc at 77 length 22 bases, Tm 62
Reverse primer details :
cAAcAAcTgATcATgAggAccA at 291 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 236 bases.
Length of sequence : 349 Consensus sequence is :
..
GTGATTTTCC GAATATCTTC TTGGAAGTAT CACCGGGTTG TCCCCTGACT
GTTATGTTGA GCTCATGATC AAGTTGGTTT CTTGTAAACC ACGCCTTCTC
TAAGAATCTA TGTTGGATAC CCCTAAGCTA GTTGGTCAAT CTAAACAACA
ACTTGGAGAG TTGGAAGATA AAAGCTTTGC CTTAACTTAG TTCATTTCAA
GGGGATCTCT CTCTCTCTCT CTCTGTGTGT GTGTGTGTGT GTGTTTGTGT
GTGTGTGTGT GTGTTGAAGA AAGATGATAT CTTCATTGAT TGGTCCTCAT
GATCAGTTGT TGGATCTATT GTCTTGTCAA AACTTTGGTT TCAGTATGG
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wmc(24d7)
Plate reference : 24d7
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 29 Oct 1998 at 16:51
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : No sequence 29.10.98.
PCR Comments :
No sequence available for this clone
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wmc446(24e7)
Plate reference : 24e7
WMC locus number : WMC446 Length of sequence : 374
Last updated : Lun 16 Nov 1998 at 10:08
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)9 33 to 50, (CA)3 52 to 57. Too difficult to make primer.
PCR Comments : 51C anneal, stuttery product.
Forward primer details :
ccAgcTAgTAcTcTATATcTAcATc at 9 length 25 bases, Tm 52
Reverse primer details :
TATTTgAAcAAgAgTTATgTgg at 103 length 21 bases, Tm 54
Expected size of amplified fragment (Chinese Spring=) 115 bases.
Length of sequence : 374 Consensus sequence is :
..
CATGCCCACC AGCTAGTACT CTATATCTAC ATCACACACA CACACACACA
CCACACACTT TCATTGTTAT CTTCTTATTT TTTGATCTAA TTCTGCACAC
GCCACATAAC TCTTGTTCAA ATACATTTTT GATACAGTTT CCCTGGTTTT
TTATCCAGTT TGTTATCTTT GAACTTCAGC TATTTTTGTA TCCAACTATT
ATCTTTGAAC TTCAGTTGTT TTTGTATCCG ACCTGCCACA CATTCTATTT
TATAGCAACT CATCCAAATT CATTTTTTAT ATCAAAGCAA ATGCCCTGTT
GAACATATAG TGACATTCTT GCAGAGTGCA TCCAAGTTCA GACGAGAAGA
AAAAACATTC AGAAGCTCAA TATT
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wmc(24f7)
Plate reference : 24f7
WMC locus number : WMC Length of sequence : 171
Last updated : Lun 16 Nov 1998 at 10:09
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)4 9 to 16, (CA)11 19 to 40. No room for primer.
PCR Comments :
Length of sequence : 171 Consensus sequence is :
..
CACACGCGCA CACACACTCA CACACACACA CACACACACA ACGACTCGGA
GCATAATCAG TGATGGCTCT ATTTTGTACA CATTTTTTAG AGATCATTTG
TTGCAGATTA TCTCCATATA TCATGTCCCA ATGAGACAAA TACATCTCTC
TTATGCATGA TTTCCAATAT T
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wmc445(24g7)
Plate reference : 24g7
WMC locus number : WMC445 Length of sequence : 285
Last updated : Lun 16 Nov 1998 at 10:13
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GGC)6 182 to 199.
PCR Comments : tested 61C anneal, no product. tested 51C anneal, no product;
Forward primer details :
AgAATAggTTcTTgggccAgTc at 38 length 22 bases, Tm 62
Reverse primer details :
gAgATgATcTccTccATcAgcA at 245 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 229 bases.
Length of sequence : 285 Consensus sequence is :
..
GGCAAGCGGA GAACTGGGCC ACAACTAGTT GTGGAGAAGA ATAGGTTCTT
GGGCCAGTCC CTCCGAGGAG AGACCGGGCC GGACTGAGCT TGCTTGTTGC
CTTTATACAA CCATCCCAGC CCGGCCGCAT CGTTGTAGAA ACGCTGCTAG
GTTATTCGCC CCAAAGAAAA AGTAAACCCC AGGCGGCGGC GGCGGCGGCA
AGTGGCGCCG ATGGGCAGCG GGTCGATCTC GTCGGACATG GAGATGCTGA
TGGAGGAGAT CATCTCCCTG CGGAGAGAGC GGAAA
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wmc(24h7)
Plate reference : 24h7
WMC locus number : WMC Length of sequence : 477
Last updated : Lun 16 Nov 1998 at 10:14
Owner : ITCF Date at Agrogene : 29.10.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 407 to 446 show homology to 2h10 bases 221 to 260 (40 in longest run, maxScore = 47). (GT)14 386 to 413. Duplicate, 2h10 has precedence.
PCR Comments :
Length of sequence : 477 Consensus sequence is :
..
GTCCCCAGTT GCACGTTGCC CATCGACTCG CAACTAGGGT ATGTGTGTGT
GTATTTATTC GTGGCCCCCA GTTGCAAGTT GACCATCAAC TCGCAACAAG
GGAGAGGTGT GTGTGTGCGT GTGTCGAGGG TCCCCGGTTG CAAGTTACCC
ATCGACTCGC TACTATGGTG AGTGTGTGTG TGTGCATGCG TGTATGTGTG
TGTGTGTTTT GTCGTGGGTC CCTAGCTGTA TGTCACGCAA CTAGGGTGTG
TGTGTGTTTA TTTGTGGCCC CCAGTTGCAA GTTGATCATC AACTCGCAAT
AAGGGAGAGG TGTGTGTGGT GTCTCGAGGG TCCCCAGTTG CAAGTTACCC
ATCGACTCGC TACTATGGTG TGTGTGTGCG TGTGCGTGTG TGTGTGTGTG
TGTGTGTGTG TGTTTTGTCG TGGGTCCCTA GCTGCATGTC AATAACTGAC
TCGCAACTAG TAGTGTGTGC ATGTGTG
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wmc447(2A1)
Plate reference : 2A1
WMC locus number : WMC447 Length of sequence : 344
Last updated : Ven 12 F思 1999 at 16:35
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 137 to 206 (complement) show homology to 24d1 bases 44 to 113 (70 in longest run, maxScore = 148). (GT)6 251 to 262, (GT)9 287 to 304. Bases 137 to 189 show homology to 26a5 bases 131 to 183 (complement) (53 in longest run, maxScore = 96). Near duplicate with 26a5, 2a1 has priority.
PCR Comments : 51C anneal, product.
Forward primer details :
AgAgATggATTggTAATTTTgT at 8 length 22 bases, Tm 56
Reverse primer details :
TTcTcTccTTAggTTTgTTTTc at 319 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 333 bases.
Length of sequence : 344 Consensus sequence is :
..
GCGGGGGAGA GATGGATTGG TAATTTTGTG CAAGAGAGAG AGGGTGGTGG
GAGGAGTCAG TCAGCCGTCG TCACATCATC CTGCTTCCAA ATGGCCCCGT
ATTCTCTAGC GCAGTGTGGT CGCCGTCTTC GATCTCCTCG ATGCCCTCTT
TGAAGATTGA GGTGTTGTGC ATGTTGGGGT GCAAAGAAGC ACAAGCGAGA
GTGGTCACCG TATAACCTTG GATGGGGATG AATTGTGTGC TCGGGGGAGG
GTGTGTGTGT GTGCTATGCT GTGTGTGTCT CTGTTTGTGT GTGTGTGTGT
GTGTGCCAGA TATTGAGAGA AAACAAACCT AAGGAGAGAA ATGG
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wmc448(2b1)
Plate reference : 2b1
WMC locus number : WMC448 Length of sequence : 247
Last updated : Mar 22 D残 1998 at 15:44
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 153 to 165 (complement) show homology to 19b4 bases 216 to 228 (13 in longest run, maxScore = 25). Bases 76 to 107 show homology to 25e11 bases 1 to 32 (32 in longest run, maxScore = 36). (GT)12 125 to 148.
PCR Comments : 61C anneal, complex product.
Forward primer details :
ATcATAcATAgATggTcgggcA at 6 length 22 bases, Tm 63
Reverse primer details :
ggccAgTcgcTcTATATcTTTg at 155 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 171 bases.
Length of sequence : 247 Consensus sequence is :
..
TCCATATCAT ACATAGATGG TCGGGCAATC CACGAGAAGA GAGGGAGGGG
TCGTAGCTAT CGATAGAGTC GAAGAGAGGA TCAAGTGGGT GGGTGCGAGA
TCGATGAGGA GAGCCATCGA AATTGTGTGT GTGTGTGTGT GTGTGTGTGC
AAGTCAAAGA TATAGAGCGA CTGGCCTACC GGAACGTTAG AGGGAACATG
TCATGTTTGT GTGTGACAGG GAGACATGAC TAGAGCGCTC GATGTAC
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wmc449(2c1.1)
Plate reference : 2c1.1
WMC locus number : WMC449 Length of sequence : 176
Last updated : Mar 22 D残 1998 at 15:48
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)32 50 to 113.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
gAgTggcAcccgTATATATggA at 22 length 22 bases, Tm 63
Reverse primer details :
ggTgTcAAcAgcTcAccAgTcT at 123 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 123 bases.
Length of sequence : 176 Consensus sequence is :
..
GGGTCTTCCA ACTCGAGGGG AGAGTGGCAC CCGTATATAT GGACATGAAG
AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG
AGAGAGAGAG AGAATCACTG CTAGACTGGT GAGCTGTTGA CACCCCGTGA
TCTTTCGATT TAGTGTAACG TAGTAC
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wmc(2c1.2)
Plate reference : 2c1.2
WMC locus number : WMC Length of sequence : 403
Last updated : Mer 6 Jan 1999 at 11:17
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 1 to 78 (complement) show homology to 14e1 bases 144 to 221 (78 in longest run, maxScore = 149). Bases 134 to 218 show homology to 25c12 bases 264 to 348 (complement) (85 in longest run, maxScore = 137). Bases 97 to 157 show homology to 24g8 bases 93 to 153 (complement) (61 in longest run, maxScore = 140). (CA)7 280 to 303, (TC)7 334 to 347, (CA)9 367 to 384. Bases 1 to 221 are duplicate with 14e1 but the second part 222 to 403 seems to be OK. Too difficult to make primer. Duplicate with 24g8, 2c1.2 has priority.
PCR Comments :
Length of sequence : 403 Consensus sequence is :
..
GTACCCCCCG CCTCTCACTC TTCCCCTATA TCCCCGAAAC CAATAAGACC
ATCCCTTTGT TTAATTCCCG CCTACACGCA CCATCGATAT ATAGTAGTCT
TCCTCGGTGT CTCTCGAACA AACACGTTCT CTTGATGTCG ACTCCTCTCA
CGCGCACACA CCTTCTCTTC CCTCTCTACG GGCATTTTCT CTCTCGCACC
CCATCGTTGG TGGCCTCTGC CATACACATC ACCTCTCTAT GATGAAGCCA
TGCACACTTA AATTACATCC GCCATAGAGG ACCCCGCGAC ACACACACAC
ACACGCACCC CCCACACACA CTAAGACTCC ATATCTCTCT CTCTCTCACA
CACACACGCA CACAAACACA CACACACACA CACATGCACG CACGCACACC
CCC
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wmc(2d1)
Plate reference : 2d1
WMC locus number : WMC Length of sequence : 137
Last updated : Mar 22 D残 1998 at 16:01
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 76 (complement) show homology to 10g4 bases 62 to 137 (76 in longest run, maxScore = 58). (GT)26 17 to 68. True duplicate, 10g4 has precedence.
PCR Comments :
Length of sequence : 137 Consensus sequence is :
..
GCATGGATGG CATGCAGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT
GTGTGTGTGT GTGTGTGTGC AAGCTAACAA CTCAAAAAAG CATCATCACA
AGCCTCCTTT CANGTCCCAG AAATAGACAG TGCGTAC
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wmc(2e1)
Plate reference : 2e1
WMC locus number : WMC Length of sequence : 364
Last updated : Lun 21 D残 1998 at 16:24
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :
Length of sequence : 364 Consensus sequence is :
..
AGACCTGGAT CAGGACCACG AAGAAACCCA TAAACATCAC GGTGGCGCAC
ATTAGAAGAC ACATGAAGAC AGACCTGCTG TGCTGTTGAG GCTCCGCCTC
CAAGGCTTCG AGATCAGCGG CAACGCCGCC GCCGCCGCTG CCGCCGCCGG
GGTTTAGGTC GTCATTGTCA AAGTATACTC TTAGCCTGTC TACCCAGGCC
TCAAATCTAT GCCGAGAGGG CTCCGCCGCG ACGAGGTTTT CCTCCAGGTC
CGGGTTCTCC AAGTCGACCA TCGAGGAGGA TCGAGGGGCG CGCGATCTTT
CCTCCTCGGC AAGGATTCCG CGGTTTCTTT CGTGACGAGC AGTTCGTATA
CCAGGCCAAA TCAG
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wmc(2f1.1)
Plate reference : 2f1.1
WMC locus number : WMC Length of sequence : 381
Last updated : Mar 22 D残 1998 at 16:06
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the first insert. Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 1 to 381 show homology to 9f3.1 bases 1 to 381 (381 in longest run, maxScore = 276). (GA)8 121 to 136. Duplicate, 9f3.1 has precedence.
PCR Comments :
Length of sequence : 381 Consensus sequence is :
..
GTACAATGAG TGCACCCAAG ATATTTAGCT TGGACTAGAG AATCATACAT
AGTGTGCGGG AGAGACCTAC AGATGGATTA TTTATGCATG CGAACTAGAA
AGACCCCCCA CACACACAAA GAGAGAGAGA GAGAGATGGA GAGAGAGGGA
GAGGGACCAA CAAGGTTTGT GTGTTGGATG CGTGCGACAT AATTGAAGAT
TGTCGACGAG AGAGAGAGCA AGAGTCCGGG AGAGGGGGAG GTCGCGTTTT
ATGCATCAAA GACTGATATA AACCATGTTT CAATATAAAC TTGCATTCAA
ATATTTAAAT TGGAGAACAT GTTGTCTGCA ATCCACACAT GAATGGAGAT
ATGTGTATAA CAAACAAGTT CATTAATCTG A
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wmc(2f1.2)
Plate reference : 2f1.2
WMC locus number : WMC Length of sequence : 410
Last updated : Mar 22 D残 1998 at 16:07
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 2 to 410 show homology to 9f3.1 bases 401 to 809 (409 in longest run, maxScore = 271). (CA)13 306 to 331. Duplicate, 9f3.1 has precedence.
PCR Comments :
Length of sequence : 410 Consensus sequence is :
..
GTGACCCTCT ACACACACTC ACACACACAC AAACACATAT CCTTAGCTGC
GAGTCGATGA GCGGGTTGCA ACTTGGGGCC ACAACAAAAC ACACACACAC
GCACCCCCCT AGTTGTGAGT TGGTTATCGA CATGCAGTTG CGGATCCTTG
AGACAAACAC ACAAACACAC AAAGACACAC ACACACATAC CCTAGTTGCG
AGTTGATGGT AAACTTGCAA TTGTGGGCCA CGAATAAAAA CACACACACA
CACATCCCTT AGTTGCGAGT GGATGGTCAA CTAGCAACTG GGGGCCACGA
ATAAACACAC ACACACACAC ACACACACAC ACCTAGTTGC GAGTCGATGG
GCGACTTGCA ACTGGGTGCC CTCAACAAAA AATGTTAATA CGTGCTGCTT
GGTTATGTAC
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wmc450(2g1)
Plate reference : 2g1
WMC locus number : WMC450 Length of sequence : 267
Last updated : Mar 22 D残 1998 at 16:19
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GCC)5 100 to 114.
PCR Comments : 61C anneal, product.
Forward primer details :
gcAggAcAggAggTgAAgAAg at 79 length 21 bases, Tm 63
Reverse primer details :
AggcgTTgcTgATgAcAcTAc at 208 length 21 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 150 bases.
Length of sequence : 267 Consensus sequence is :
..
CCGTCGGAGT GCGTCATGCG GACTCACCTG TCCGGCAGGA AGCACACCAA
CAAACAGAAG GCCGCCGCTG ACGCCGGTGC AGGACAGGAG GTGAAGAAGG
CCGCCGCCGC CGCCACGATC GGCAGCCCAT CCAAGGTAGC CGCCTCCATC
GCGGTCAACA GCAGCGATGA CTCCATGAAG AAACCAGCAA CGGGAGAGAT
GGTGGTGGTA GTGTCATCAG CAACGCCTCA AGTGGACGTC GCCGCCCCGG
TCTGCGCCCG TGTCTCC
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wmc451(2h1.1)
Plate reference : 2h1.1
WMC locus number : WMC451 Length of sequence : 189
Last updated : Mar 22 D残 1998 at 16:33
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 145 to 165 (complement) show homology to 11d6 bases 62 to 82 (21 in longest run, maxScore = 36). (GC)5 110 to 119, (AC)6 120 to 131.
PCR Comments : tested 61C anneal, no product; 51C anneal, polymorphic on acrylamide.
Forward primer details :
ccccgAgAAATcAgAAcAgAc at 4 length 21 bases, Tm 63
Reverse primer details :
cTTgcTAgggTTTTgAgcgAg at 137 length 21 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 154 bases.
Length of sequence : 189 Consensus sequence is :
..
GTACCCCGAG AAATCAGAAC AGACCACGAA AACCTAATTC CACCTCTGGA
ACCTTCTGTA TCCGCGGGAT CCCATCTTGT AGCCTTCGCC GGCGCTCCGC
CGGGGGCGTG CGCGCGCGCA CACACACACA CCCTCCCTCG CTCAAAACCC
TAGCAAGGTC CCCGCCGCCG CTGCCGCCGC CGCCGCAAG
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wmc(2h1.2)
Plate reference : 2h1.2
WMC locus number : WMC Length of sequence : 191
Last updated : Mar 22 D残 1998 at 9:50
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 27 to 117 show homology to 1B1 bases 244 to 334 (91 in longest run, maxScore = 67). (CA)15 121 to 150, (CT)15 91 to 120. Duplicate, 1b1 has precedence.
PCR Comments :
Length of sequence : 191 Consensus sequence is :
..
CATGAGTTTC TAACTGGGTG TTTGCCCGTT GACAATGCAA TAGTGATTCG
CTTTACTTAA CCAACTTTGT GTTGAAATAT ATAGCATAAT CTCTCTCTCT
CTCTCTCTCT CTCTCTCTCT CACACACACA CACACACACA CACACACACA
GAGCGCTTAA GCATTGTCTG AATGATCGGT GCTCACAGTA C
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wmc(2a2)
Plate reference : 2a2
WMC locus number : WMC Length of sequence : 320
Last updated : Mar 22 D残 1998 at 16:39
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 100 to 143 (complement) show homology to 6b12 bases 182 to 225 (44 in longest run, maxScore = 57). (GT)4 112 to 119, (GT)6 121 to 132, (GA)6 133 to 144, (GT)7 240 to 254. Duplicate, 6b12 has precedence.
PCR Comments :
Length of sequence : 320 Consensus sequence is :
..
GGCTTTCCCT TGTTCATCCT TTTCTCCCCA CAAGATAAAC TACTGATATA
AATGCATCTT GATGTTCCAT GCAATGCATG AGCATCTTGC TAGTTGTTTC
AAAAAGCCCA TGTGTGTGTG GTGTGTGTGT GTGAGAGAGA GAGAGGGAGA
GAGGAGGAGG ATGGAGCGCG TGTGTGACAA AGACACAATG AGTGTGTGTG
CAAGAGGTAT CACCATAAAA TGAGGCGATA GTGTGTGTGT GTGTGCACGA
TCGAGAGGGC GTGTTTCAAG AAGGGACGGA AAATCTACAT AGATACAAGG
AGCGCGAGAG AGACATGTAC
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wmc452(2b2)
Plate reference : 2b2
WMC locus number : WMC452 Length of sequence : 461
Last updated : Mar 22 D残 1998 at 16:49
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. (GCC)6 296 to 313.
PCR Comments : 61C anneal, product.
Forward primer details :
cgccTccTTcTggTTTATcAc at 230 length 21 bases, Tm 63
Reverse primer details :
ccTTcTTccccTccAAATcTc at 393 length 21 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 184 bases.
Length of sequence : 461 Consensus sequence is :
..
GTACCTGAGG GAGGAGATCG GAAGCCGGTC AGAACACTTC CCTGCGAAAT
CCGTCGGGAG CGGGGAACGA GCGGAAAAGG TAGGTCGGGG GGTGCGGGTC
TGATACTCAC TGGGCGAAGA CGGCGAGGAG GTCGCCCTCG GTGAGGTCGA
AGGGGACGCC GCCGACGAAG ACGTAGGCGG AGCCCCTGAA CTTGGCGTGC
CAGGAGGCGT CCTCGCTGAG GCCCAGCGCC GCCTCCTTCT GGTTTATCAC
CTGCGTCCGC TTCACCTGCG TCAGGGGATT CATCTTCCCC GACTCGCCGC
CGCCGCCGCC GCCGAGGTGG GTCGGGTCCC TCCCCTACCT AGAGGGAACA
AGCGGTGCTC GGGCGGAATT TCTCGAAGGG GCGGCGGATT GGGAGATTTG
GAGGGGAAGA AGGTGGGGGA TTTTTTTGGG GGGTTAGGGT TCTTGGCTAG
TCCAACAGCC T
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wmc453(2c2)
Plate reference : 2c2
WMC locus number : WMC453 Length of sequence : 480
Last updated : Mar 22 D残 1998 at 17:04
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)35 119 to 188.
PCR Comments : 61C anneal, polymorphic on acrylamide (though complex).
Forward primer details :
AcTTgTgTccATAAccgAccTT at 73 length 22 bases, Tm 61
Reverse primer details :
ATcTTTTgAggTTAcAAcccgA at 238 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 187 bases.
Length of sequence : 480 Consensus sequence is :
..
ATGCATGACA TAATAAACTC CCCCCCAGAT AATGTATGCA TAGAATATGT
GACAATCCAA GGATTGGTAT ATACTTGTGT CCATAACCGA CCTTTAATAA
ATTAATAGTG AATTTGAACA CACACACACA CACACACACA CACACACACA
CACACACACA CACACACACA CACACACACA CACACACACC GGTGTGTCCC
CCTTTCTCTT TGCCGATGGT ATTTGTATTT CCAGCTTTCG GGTTGTAACC
TCAAAAGATT CAAACTAAAG TTACATAAGA GCAGTGAGTT GGCTGGTATG
ACAAGGCACG AGCTCGTCTC TTCTCATGCA GAGATAGCGT CTCCTTTGTG
GTTTACACAG GAAACACCAC CTGACGTGTA TCCTTGCCCC GACCAAAGAG
CCTTCACTCG CTGACTATCT CCCAGCGCTC ATTGCCAAGA TTGGAAGAGA
CTAGCTTGTT GTCCCACCCA AAATGGGTAC
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wmc(2d2)
Plate reference : 2d2
WMC locus number : WMC Length of sequence : 137
Last updated : Lun 30 oct 2000 at 16:59
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 3 to 53 show homology to 12h9 bases 1 to 51 (51 in longest run, maxScore = 72). No substantial microsatellite. Duplicate, 12h9 has precedence. Bases 40 to 89 show homology to 26g5 bases 1 to 50 (complement) (50 in longest run, maxScore = 42). Duplicate with 26g5, 2d2 has priority. Bases 1 to 49 show homology to TW6d10.3 bases 5 to 53 (49 in longest run, maxScore = 40).
PCR Comments :
Length of sequence : 137 Consensus sequence is :
..
CAAACACACA CAGACACGCA CAAACACCCA TTTATCTGGA TCCTCATCTC
TCCTCATGTC TGCATGTGTA TCTCACACAC TCACTCTCTA ATTATGTATA
TGTGTCTACA TGTTACACAA CACAAAGCCC CATGTAC
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wmc(2e2)
Plate reference : 2e2
WMC locus number : WMC Length of sequence : 213
Last updated : Mar 22 D残 1998 at 17:08
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert ( NB 3' linker corrupted). Bases 148 to 198 (complement) show homology to 7A1 bases 190 to 240 (51 in longest run, maxScore = 48). (GT)11 49 to 70. Duplicate, 7a1 has precedence.
PCR Comments :
Length of sequence : 213 Consensus sequence is :
..
GTACAACATT AATAGGTATT TTATATCTAC TATATGAATT ACTCTCTCGT
GTGTGTGTGT GTGTGTGTGT ATTTCTTCTC TAGATACCAA GAACCATTTT
TCGTGATTTA CATTTACACA CAAACATCCA ATGTTACCGA CACACATGTC
ACTCGCATGC CTAGCTGCCT GACATGTTTT TGTGACAGAT TGCTTGGCGG
TTGCTAGTAG TAC
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wmc(2f2)
Plate reference : 2f2
WMC locus number : WMC Length of sequence : 533
Last updated : Mar 22 D残 1998 at 17:09
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert. Bases 475 to 494 (complement) show homology to 3e12 bases 247 to 266 (20 in longest run, maxScore = 66). Bases 21 to 37 show homology to 21d10 bases 130 to 146 (complement) (17 in longest run, maxScore = 57). Bases 66 to 95 show homology to 21g11 bases 78 to 107 (complement) (30 in longest run, maxScore = 38). (GT)8 95 to 110, (GT)8 401 to 416. Duplicate with 3e12, 3e12 has precedence.
PCR Comments :
Length of sequence : 533 Consensus sequence is :
..
GTACCTAGTT GCATGTCAAG CATCGACTCG CAACTAGTGT GTGTGCGTTT
GTTCAGGACC CCCAATTGCA AGTTTCCCAT CGACTCGCAA CTAGGTGTGT
GTGTGTGTGT TTACTCGTGG CCCCTAATTG CAAGTTGACC ATCCACTCGC
AACTAGGGGA TGTGTGTGTG TGTCGAGGGT CCCCAATTGC ATGTCGATAA
CCGACTTGCA ACTAGAGGTG TGTGTGTCTT TTTGTCAAGG GGTCCCCAGT
TGCATGTCAA GCATCGACTC GCAACTAGGG GTGCGTGTTT GTTGAGGACC
CCTAGTTGCA AGTTGCCCAT CGACTCGCAA CTAAGGTGTG TGTGTGTGTT
TATTCGTGGC CCCCAGTTGC AATTTGACCA TCAACTCGCA ACTAAGGGAT
GTGTGTGTGT GTGTGTCGAG GGTCCTCAAT TGCATGTTGA TAACCGACTT
GCAAGTAGGG GTGTGTGTGT GTGTCTTTTT GTCAAGGGGT CCCCAGTGGC
ATGTCAAGCA TCGACCCATA ACTAGGGGTG TAC
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wmc454(2g2)
Plate reference : 2g2
WMC locus number : WMC454 Length of sequence : 444
Last updated : Mar 22 D残 1998 at 17:22
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 96 to 105 show homology to 6b11 bases 325 to 334 (10 in longest run, maxScore = 20). (TG)6 61 to 72, (GT)5 80 to 89, (GT)8 126 to 141, (GT)6 162 to 173, (GT)21 246 to 287.
PCR Comments :
Forward primer details :
ATTATTTAgAAcAgATTgcggT at 35 length 22 bases, Tm 56
Reverse primer details :
AAcATggTgcATATTTAgTgAT at 328 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 315 bases.
Length of sequence : 444 Consensus sequence is :
..
GTACACACTG TTTCATGCCG ATTTGAAGCC AATGATTATT TAGAACAGAT
TGCGGTTTCA TGTGTGTGTG TGCGCGCGCG TGTGTGTGTG CTTGTGTGTG
TGTGCGTGCG TGCGTGCATG CGTTCGTGTG TGTGTGTGTG TGCGTGTGCG
TGCATGCGTT CGTGTGTGTG TGTGCGTGTT CGTGCGTGGG TTATTGCGTG
TGTTCTTGCG CGTGTTCGTG CACGTGCACG TGTGCGTGTG CGTGCGTGTG
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTCTC ATTCTACTAT
GCAGCTAAGA TTTTACACCG GACGAGCATC ACTAAATATG CACCATGTTG
CATCAGGCAC TTTTCTTGTT AATTCACATG AGTATTGAGT AGTCCAATTT
TAACTGACCA GCCATCAATT TGAATGTGTT GTAGGAAATT TTCG
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wmc(2h2)
Plate reference : 2h2
WMC locus number : WMC Length of sequence : 246
Last updated : Mar 22 D残 1998 at 9:41
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 155 to 173 (complement) show homology to 22g9 bases 120 to 138 (19 in longest run, maxScore = 38). No substantial microsatellite.
PCR Comments :
Length of sequence : 246 Consensus sequence is :
..
GGGATCTTTG GGAAAGGGGG TGTTTCGCAG GTGTCATCTA AGAAACTCGG
GCGGCAAGTG TATGTATATC GGTGGGTAAA CTGCACGGGC AGACCGGCAG
AGATTGACGA TGGAGGCGGC GTGGCGGCGG CGGCGACCTA GCTAGGTTTG
GAGAGAGGGA GGGGTGGTGT GTAGTGTGTG TAGCGGGGGA TTTGGGGTCT
GTGTGGAGGG GGTCCAAGGT GGTGTTTGAG GAAATGCCGC GGGTAC
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wmc(2a3)
Plate reference : 2a3
WMC locus number : WMC Length of sequence : 603
Last updated : Mar 22 D残 1998 at 17:26
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 183 to 307 show homology to 6f7 bases 158 to 282 (125 in longest run, maxScore = 79). (CA)30 159 to 218. Duplicate, 6f7 has precedence.
PCR Comments :
Length of sequence : 603 Consensus sequence is :
..
GTACTAAACC TGCAAACACA AAGTCAACGT TTCCTTTCAT AAGTAAATCC
TCCCTGACGG CCTCGATNAC CTGCATGAGA CAACCGGTCG ACGACAAGGC
ACCATCATAC ACAACTTTGT AGGAGCAATT ATCAGGGCCG GCCCTGGTGA
CACACACGCA CACACACACA CACACACACA CACACACACA CACACACACA
CACACACACA CACACACACC CCTTTAGCAC TGGTCAGGCA CAAACAGCTG
CAAAATATTC AGTGGAACGG AACTACTCCT AATAGGCAAC GGACTAGCAA
AGAAGCTTAA ACAACTAACA AAACACTATG TCTCAAAGAC ATGATGCAAC
AATTGAGTTC ACTACAGCCA ACAAAACCAA TATATAGCAG GGGATAAGAA
CAAATTCTGT GGATACAAAT TATTTCGATG ATGCCGAGGC AAACATTGTG
TGACTTGCTA GTTATACTGC TGGCGTGAGT TCAGAGAGAG GAGCGTCCTC
AAGATACCAG CAAGAAAGCC AATGATGGGC AGCATCGTGA GGATGATTTT
TGTGTTGTCT TGGATGAAGC CGTAGACCTT GATCCAGCTC GTCGTCTCCT
TCT
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wmc(2b3)
Plate reference : 2b3
WMC locus number : WMC Length of sequence : 117
Last updated : Lun 21 D残 1998 at 16:24
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end ( NB 5' linker corrupted ). No substantial microsatellite.
PCR Comments :
Length of sequence : 117 Consensus sequence is :
..
ATAGTCTGAC GGTAGCCAGG CGGCAACGCC CGTGTCAGAG TGCGGTTACT
CCTGCCCTGG CATCATGGTC TGAGGAAGCA TCGAAATAGA TTAAATTTTA
AATAAAAAAG AGGGTAC
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wmc(2c3)
Plate reference : 2c3
WMC locus number : WMC Length of sequence : 409
Last updated : Mar 22 D残 1998 at 17:28
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 140 to 155 show homology to 10b12 bases 140 to 155 (16 in longest run, maxScore = 33). Bases 30 to 231 show homology to 2d3 bases 30 to 231 (202 in longest run, maxScore = 304). No substantial microsatellite. Duplicate with 2d3, 2c3 has priority.
PCR Comments :
Length of sequence : 409 Consensus sequence is :
..
GTACAAGGCC ATGGCGGCAG TCGAGTATCA TGAAGCCCTT GCCGTGCTTG
AACTGCAAGG AGAAACGCCC GGGCGGGACA CGATTGGGGC CCTCCAAAGC
AGGTAGGAAG GAGATGCCGT CAAGCTGTTC GACGAAGCAN CCGAGGAGGG
GAGGGCTGCG GCAGTGGAGG ATGCGGAAGC GGCGGAGGAA GCCGGGGTCG
GAGACAAGGC GGCGCCAGCG TTTGGATACG AAGGAGGCGC GAGGGAAGGA
GGACGGCTGC GGCGGGAGGC GGAAGAAGAT CTCGGGGAGT AGGTTCTCGT
CCTCCAGCGG CGGCGCCGAC GAAGAACGGT GGCGGCGGCT GCGGCTGCTG
CTGCTCATCT CGCCCTACTC ACTCTCAAGG ACAGAGAAGA TGTGGACTGG
ATGCAATCC
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wmc(2d3)
Plate reference : 2d3
WMC locus number : WMC Length of sequence : 409
Last updated : Mar 22 D残 1998 at 17:28
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 30 to 231 show homology to 2c3 bases 30 to 231 (202 in longest run, maxScore = 304). No substantial microsatellite. Duplicate, 2c3 has precedence.
PCR Comments :
Length of sequence : 409 Consensus sequence is :
..
GTACAAGGCC ATGGCGGCAG TCGAGTAGCA TGAAGCCCTT GCCGTGCTTG
AACTGCAAGG AGAAACGCCC GGGCGGGACA CGATTGGGGC CCTCCAAAGC
AGGTAGGAAG GAGATGCCGT CAAGCTGTTC GACGAAGCAG CCGAGGAGGG
GAGGGCTGCG GCAGTGGAGG ATGCGGAAGC GGCGGAGGAA GCCGGGGTCG
GAGACAAGGC GGCGCCAGCG TTTGGATACG AGGGAGGCGC GAGGGAGGGA
GGACGGCTGC GGCGGGAGGC GGAGGAGGAT CTCGGGGAGT AGGTTCTCGT
CGTCCAGCGG CGGCGCCGAC GAGGAGCGGT GGCGGCGGCT GCGGCTGCTG
CTGCTCATCT CGCCCTACTC ACTCTCAAGG ACAGAGAAGA TGTGGACTGG
ATGCAATCC
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wmc(2e3)
Plate reference : 2e3
WMC locus number : WMC Length of sequence : 313
Last updated : Mar 22 D残 1998 at 17:33
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. (CA)6 36 to 47, (CA)5 51 to 60. Too difficult to make primer.
PCR Comments :
Length of sequence : 313 Consensus sequence is :
..
GTACCCACAC ATACACACAT GCATAGACAC ACACGCACAC ACACACACTA
CACACACACA TACACACGCA GTGCTTACTC TTGAGAACAG AATTCTCATC
TTTCAGTTCA CGTATCTCCC ACTCAAGCTC CAACACCTTT TGCATAAATC
TTGCTTATTT TCCTGCAAGG GACAAAAAGG GATAAACTGG ATCTTAGGCC
TCTTTACTTT ATTGGGGAGA CTCGACTTCT TAAATTCAAT GTGCATATCC
CTAGGTTGGG GCAACGGAAC TCCATCCTCC TCCGAGCTCG TCTCCTCCTT
CCCCTCAAGA TCA
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wmc(2f3)
Plate reference : 2f3
WMC locus number : WMC Length of sequence : 471
Last updated : Lun 8 F思 1999 at 10:07
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 268 to 298 show homology to 22g7 bases 15 to 45 (31 in longest run, maxScore = 49). Bases 308 to 334 show homology to 21e9 bases 46 to 72 (complement) (27 in longest run, maxScore = 28). (GA)10 179 to 198, (GT)8 276 to 291, (GT)12 362 to 385, (GT)11 388 to 409. Duplicate, 22g7 has precedence. Bases 210 to 266 show homology to 26b5 bases 164 to 220 (57 in longest run, maxScore = 31).
PCR Comments :
Length of sequence : 471 Consensus sequence is :
..
GTACCCAGCT GACGAGCGGA GAGGCTGACC AGTGGGCCTA GTTGACTGGG
TCAACTGCCC AGTCAGCAGA GGCTGACTGG TGGAGCCAGT AGGTCCAGTC
AACAGTCAAC AGTTTGACTG TTGACCGGTC AACCTGACCA GTGGGCCCCA
TGTGTGTGTG GGGTGTGTGT GTGTGCGTGA GAGAGAGAGA GAGAGAGATG
GAGTCTTACT GTGTGTGTGA GAGAGAGAGA TGGAGTCTTA GTGTGTGTGT
GGGGGGGGGG TGCGTGCGTG CGTGCGTGTG TGTGTGTGTG TTTGTGTGTG
TGTGTGTGAG AGAGAGAGAT GGAGTCTTAG TGTGCGTGTG TGGGGGGGTG
TGTGCGTGCG CGTGTGTGTG TGTGTGTGTG TGTGTGCGTG TGTGTGTGTG
TGTGTGTGTC GCGGGGTCCT CTGTGGAGGA TGTAATTTAA GTGTGCATGG
CTTCATCATA GAGAAGTGAT G
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wmc(2g3)
Plate reference : 2g3
WMC locus number : WMC Length of sequence : 225
Last updated : Mar 22 D残 1998 at 17:50
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 76 to 107 show homology to 5b5 bases 136 to 167 (32 in longest run, maxScore = 79). (AC)8 61 to 76,(AC)5 129 to 138, (AC)6 96 to 105, (CA)4 113 to 120. Duplicate, 5b5 has precedence.
PCR Comments :
Length of sequence : 225 Consensus sequence is :
..
CACGCACACA TCTCCTAGTT GCGAGTCGAT GGGCAACTTG CAACTAGGGG
TCCTCAACAA ACACACACAC ACACACCCTA GTTACAAGTC GGTGGTTGAG
ATGCAACTGG GGGGCCCTTG ACAAAAAGAC ACACACACGC GCGCACTTCT
AATTGCGAGT CGATTATAGA CATGCAACTA GGGACCCATG ACAAAACACA
CACACGCACA CGCACACACA TGTAC
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wmc(2h3)
Plate reference : 2h3
WMC locus number : WMC Length of sequence : 366
Last updated : Lun 21 D残 1998 at 16:24
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :
Length of sequence : 366 Consensus sequence is :
..
GATGCAACTT TTGGCGCAAA GACTGACTCT TTTGTCGCGA CCGACCATTG
CAGGGGTGGA GCGAGCGGAG ACGGTGCTCC GGCCAACGGC GGCGGAAGGA
CGGCGGCGAA CTCCGGCGGG TAAGATCTTC TTCTTCTCTT CTTCTTGTTC
TTTTTCTTCT CGATATGCAA CTTCTCGATG ATTTCACAGA AAGACTGACC
CCTTGTCGTG CGATGGACCG TTGCAGGGGG CAAGCGACCG GAGGCGGGGC
TCCAGCCAAC GGAGGCGGCG GCGGCGCGGT CACCGGCTCT GGCAACGGGT
AAGAACTAAG AGCCCATGCA TCTTTCGTCT TTTCGTGCTT CCTCGTCGCC
GAGCGCGCTA CCGTAC
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wmc(2a4)
Plate reference : 2a4
WMC locus number : WMC Length of sequence : 150
Last updated : Lun 21 D残 1998 at 16:24
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends ( NB 5' linker corrupted). No substantial microsatellite.
PCR Comments :
Length of sequence : 150 Consensus sequence is :
..
GAAAAGGTAG CTGTCTAGCA CATCCGACGC GCGCTACCTT GCCATCTAGG
GTGCCTCCTA GGTATCATGG GTTGTATACA AGGGAATGAA GCGGCAAAAT
CCTCAACGCG ACCTTCATTG ACAAGCACAC TAGATTGACC GAGGAACACC
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wmc455(2b4)
Plate reference : 2b4
WMC locus number : WMC455 Length of sequence : 361
Last updated : Mer 23 D残 1998 at 10:11
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert. Bases 232 to 262 show homology to 12c7 bases 55 to 85 (31 in longest run, maxScore = 24). (CA)6 219 to 230, (CA)15 233 to 262, (CA)5 265 to 274.
PCR Comments : 61C anneal, product.
Forward primer details :
gcgTcATTTccTcAAAcAcATc at 184 length 22 bases, Tm 63
Reverse primer details :
AgAAggAgAAgTgccTcAccAA at 320 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 158 bases.
Length of sequence : 361 Consensus sequence is :
..
GTACTTGCAG ACGGAAGCTT GACCGACTAA CGTGATTTCA TGNGATATCT
TGTGTGTGCG TGTAAGTATC GGCGTCGTCG TGTTCATGGG AAGCCAACCG
GCTGGGTCGG AACGTAATGC GTGGTCGTGT TCATCGGGAG GTCTTGGACG
GAACAGGCGA TGGAAATGAG GCCTGGCGTA CCCGCGTCAT TTCCTCAAAC
ACATCCCCCG TCCCCCTCCA CACACACACA AACACACACA CACACACACA
CACACACACA CAAACACACA CACAAGTCAC AGTAGGAGTG GCACCTAGAA
ACACTTGACG TGCAACAGAT TGGTGAGGCA CTTCTCCTTC TGGAATGCTA
GAAGGAGGTA C
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wmc(2c4.1)
Plate reference : 2c4.1
WMC locus number : WMC Length of sequence : 389
Last updated : Mer 23 D残 1998 at 10:12
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the first insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)11 16 to 37. No room for primer.
PCR Comments :
Length of sequence : 389 Consensus sequence is :
..
CCAAAAACAA AAAATCACAC ACACACACAC ACACACACTA ACATACGGGA
CCAAGGCACA ACAACATGCA GGGCCATGAG GATAACACCG GAAACCCACA
GAGCAAGCAG GTGCTAAACG AACGAGAAGA ACGACATGCT TTTACCCGCC
ATATGAAATG GGGAGACACC CATGCAATGG GCTCACCCAC CAAGGTGGCC
CTTCAAGGAA GCAAGGACCC AGCTCCAATG CGCGATGCCG ACGATGAACC
ACCAAGGGAC GCTCAAGTGC GGGCTTGTGA ATGGCCTTCA GAGGAATTCA
TGGATCGAGC AGGAATCAAG AAAGAATTTA ACGCATATGT GCATAACGCT
GATCTTGTGA GCTTCGAGGC AGATAAGTGC CTTTAGTAC
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wmc(2c4.2)
Plate reference : 2c4.2
WMC locus number : WMC Length of sequence : 407
Last updated : Lun 5 juil 1999 at 17:23
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the second insert. Trimmed to Rsa1 at both ends of insert. Bases 308 to 327 show homology to 8h8 bases 103 to 122 (20 in longest run, maxScore = 60). (GC)6 5 to 16, (GT)6 17 to 28, (GT)5 96 to 106, (GT)7 160 to 173, (GT)14 261 to 288, (GT)5 343 to 352. Too difficult to make primer. The microsatellite is embedded in small minisatellite repeat structure. Bases 272 to 294 show homology to 26c11 bases 96 to 118 (23 in longest run, maxScore = 65).
PCR Comments :
Length of sequence : 407 Consensus sequence is :
..
GTACGCGCGC GCGCGCGTGT GTGTGTGTAT GTGTGTTTTA TTCGTGGCCC
TCAGTTGCAA GTTGACCATC AACTCGCAAC TATGGGGATG TGTGTTGTGT
GTGTGTCGAG GGTCCCTAAT TGCATGTTGA TAACCGACTT GCAACTAGGA
GTGTTGTATG TGTGTGTGTG TGTCTATTTG TCAGGGGTCC CTAGTTGCAT
GTCAAGCATC GACTCGCAAC TAGGGTCCCA AGTTGCATGT CGATAATCGA
CTCGCAATTG GTGTGTGTGT GTGTGTGTGT GTGTGTGTTA TTTTTATCAA
AGTGTCCCCA GTTGAATGTC AACCATCGAT TCACAACTAG GGGTGTGTGT
GTATTTGTTG AGAACCCCCA GTTACAAGTT GACCATCCAC TCGCAACTAG
GAGGTAC
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