Wheat microsatellite WMC 25/06/01

wmc(2d4) Plate reference : 2d4
WMC locus number : WMC Length of sequence : 281
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 121 to 150 show homology to 3h9 bases 112 to 141 (30 in longest run, maxScore = 66). No substantial microsatellite.
PCR Comments :



Length of sequence : 281  Consensus sequence is :

..

GTACATTAAG AGAGAAAAAC GCAAGTTCGT TCAAACCTTA TCTTTTATTC 
AGTCACACTG TGAATTTATA TTCATATGAT CGAGAATTCT AGATACATTA 
TTAAACCCCA AAAAAAATAT TTTCCGGCGG CGGCGGAGGC GGCGTGCCGC 
GCCGTCGTTG TCGTTGTCAT CCTCCGGGAC ACCATTGTTG AAGTCTTCAA 
GGCAGCACAA AATGTTCTTC ACGTGTAAAT CAAACGTCAT GTCGTCGCAC 
GGGTGGACGA CAAGAGGTAG CGGTCGACGG C

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wmc(2e4) Plate reference : 2e4
WMC locus number : WMC Length of sequence : 132
Last updated : Mer 23 DŽc 1998 at 10:34
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 92 to 113 (complement) show homology to 6g1 bases 227 to 248 (22 in longest run, maxScore = 28). (CA)8 58 to 73, (CT)8 18 to 33. No room for primers.
PCR Comments :



Length of sequence : 132  Consensus sequence is :

..

CACACACTAA GATCCATCTC TCTCTCTCTC TCTCACATAC ACACACACAA 
ACACAAACAC ACACACACAC ACACGCACGC ATGCACGCAC TCCCCCCCCA 
CACACACTAA GACGCCATCT CTCTCTCACA CA

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wmc456(2f4) Plate reference : 2f4
WMC locus number : WMC456 Length of sequence : 292
Last updated : Mer 23 DŽc 1998 at 10:45
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 103 to 278 show homology to 5g9 bases 240 to 415 (176 in longest run, maxScore = 124). (CA)16 103 to 134. The homology concerns just one side of the microsatellite.
PCR Comments : tested 61C anneal, no product; 51C anneal, product -polymorphic around 320-360, possibly also 150-160.
Forward primer details :
gggTTcAgTAAcgTAgccAgTg at 14 length 22 bases, Tm 63
Reverse primer details :
ggAcATTTggATAAccgcTcTT at 165 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 173 bases.



Length of sequence : 292  Consensus sequence is :

..

NGATCGACCA CTCGGGTTCA GTAACGTAGC CAGTGCAATC ACTCGGGTTC 
AGTAAGCGAT AGCCTCGCTC GGGTTCAGTT AGAGCCCAAC GCCTCGCACA 
CGCACACACA CACACACACA CACACACACA CACACTAGGT TTTGTAAACA 
TTTGGATGTA GCACAAGAGC GGTTATCCAA ATGTCCTTGA CCGTGGACAC 
GGATATTTTT GATTAGTTTT ACACTCTACA GAGGTTGCAC ACTTTACCCA 
CAAGATCCGG GAAGCTTTCA TGTCATCCAC GACCCGCGGT AC

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wmc(2g4) Plate reference : 2g4
WMC locus number : WMC Length of sequence : 99
Last updated : Mer 23 DŽc 1998 at 10:52
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)7 61 to 74. Too difficult to make primers, and too small.
PCR Comments :



Length of sequence : 99  Consensus sequence is :

..

TGTGACCGAC ATGAACTGGG CCCTTATCCG CGGGCGCACT ATCGATAGAT 
TTAACGCGCG CACACACACA CACACTCTAC AGAGGTTAGC ACACTGTAC

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wmc(2h4) Plate reference : 2h4
WMC locus number : WMC Length of sequence : 165
Last updated : Mer 23 DŽc 1998 at 10:53
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 163 show homology to 3f11 bases 1 to 163 (163 in longest run, maxScore = 126). No substantial microsatellite. Duplicate, 3f11 has precedence.
PCR Comments :



Length of sequence : 165  Consensus sequence is :

..

GGCGGCGGCG GCGGAGACGG GGACGGGCAG TTGTATCCGC AACCGCGAAC 
GGTGTAGACC ATCAGTGCCA TGGCAAGGAG CAGGAGGGCG AGCTTGGACG 
ATGGCGGCGG CGCCATTGCT AGAGCTTTGA TTGAGGAAGC TAGTCGCATG 
TGTATGTGAT TGTAC

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wmc(2a5) Plate reference : 2a5
WMC locus number : WMC Length of sequence : 611
Last updated : Mer 23 DŽc 1998 at 10:55
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 360 to 374 show homology to 6h11 bases 297 to 311 (15 in longest run, maxScore = 27). (GCC)5 1 to 15. No room for primers.
PCR Comments :



Length of sequence : 611  Consensus sequence is :

..

GCCGCCGCCG CCGCCTTAAG CCGCCACCGC CGCCCTAAGC CGCCGCCCGG 
AGCCCCGCCT CTCGAGGTAG CCGCGCCGTC TCTTCTTTTC CTCGGTTCGG 
TTTTTCTTCT AGAAAACTGT TCCGGTCCTT CTCTTTTTTT TCTTCCGGTT 
TTTCTTCCGG CTTTGTTTCG GTTTTCTTCC GAAACCCAAG ATCGGTTTTC 
ATTCTTCTTT CGGACCGTTC GTCTCAACGA ACATGATCAC CGATTTTTCC 
CGGTTAATGA CGCCCGTTCA TTTAACCGTT CGTCTCTTTC TTTTCGTCAG 
ATTTATTCCG CGATCGCGGT CTCGGATCCG ATCTTCGTTC TAGTCTTTCT 
TCCCGCTCGT TTATCGGAAT CAGGTGATTC AAGTGCCTGG AGTTTCGTTT 
CGAAACCACC GTTCCGACTA ATCAACTTGA ACAAGTTTTT GCCACAATAA 
GATTTGACCT AGTTTCAACC TGCTAAAACC GACTTGTTTT CTTCCGCCGT 
TTGTTTTTCC GTCGTCTGTT CGGTTCTTTA TTTCTTTGCA ACCGGAGTTC 
TTAAGTTGAA CTTTCTGGTT TGTTCTATTG TTCGAGTTTT ACCTGTGCAT 
TAGATGAGTA C

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wmc(2b5) Plate reference : 2b5
WMC locus number : WMC Length of sequence : 499
Last updated : Mer 23 DŽc 1998 at 10:57
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 113 to 121 (complement) show homology to 3e4 bases 904 to 912 (9 in longest run, maxScore = 20). No substantial microsatellite.
PCR Comments :



Length of sequence : 499  Consensus sequence is :

..

GTACCCGCTG AACCAGCGCC AGACAGAANT ATCTCCCTCC TCACCCCCTT 
TCTCTCCCTC GTCTCTTCCC ATCCCGCTCC TCTTCTCCAT GCCGCCGCCA 
CCGCCGCTCC TTCTCCTCCT CTCGGCCGCC TGCCTCCCCC GCCTCCAACA 
CCTCCTCCTC TCTTTGCCTC CTCACCCAAC CAGCACAGCC AGACCACGCG 
ATGTGGATGA GGAGGCAACA GATCGGACGA GGAGGCAACA GATCGGGAGG 
CGGGCAAGAG GAACCAGGTG GTTCTCCTTC CATGGCCGGG CCTCGACCAG 
CCGCATCTTC CTCCTCCCAG TCCGGCTGAG AGGAGTGGCG GATCCGCCTC 
CTCAAGTGAG CAGGGGCGCA CGGGGATTCG ATCCAGGGAT CTCCTTGCGA 
GAGGCCGCCA CCGTACCGAC TCCAACCTGC AGCCTATTCC AACTCAGGTT 
TGTCTTTGCT CTACTTGGTA GGCCACTGGC AGGAACATGT TAGTCCACG

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wmc(2c5) Plate reference : 2c5
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : No sequence 2.11.98.
PCR Comments :
No sequence available for this clone
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wmc457(2d5.1) Plate reference : 2d5.1
WMC locus number : WMC457 Length of sequence : 427
Last updated : Mer 23 DŽc 1998 at 11:02
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multple inserts in this clone, this is the first insert. Trimmed to insert at both ends. (CA)13 342 to 367.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
cTTccATgAATcAAAgcAgcAc at 243 length 22 bases, Tm 64
Reverse primer details :
cATccATggcAgAAAcAATAgc at 380 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 159 bases.



Length of sequence : 427  Consensus sequence is :

..

CATACTTACA GAGGTTCTTG AGAGAGTAAA TAGTAGCATC AAGTCGACGT 
AATGCAGGCA TATGACTATG GACATCTTTC GGTGGCCTTT TTTCCCAACC 
ATCCACCATA CTTACAAATA TTAATTTGCC CTGCAAAGAA TAAGTTTCAA 
ATTCAATCTA TTAAAAAACA TAGAAAATAG AATGCAAATA ATAATAGTGA 
TAGAATGTTA GGGTTTGAAC TTTACTACTT CAAGGACTCC TGCTTCCATG 
AATCAAAGCA GCACATTTTT TTTAAAGAGA TTAACTTACA ACCAAGAAAA 
AATCTGGTCC ACCGGCTGGA GTAGGAATCT GAACACATAA ACACACACAC 
ACACACACAC ACACACAGAG AGAGCTTATG CTATTGTTTC TGCCATGGAT 
GGCTAGCTCC TGTTCTCCCG TGCTTCT

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wmc(2d5.2) Plate reference : 2d5.2
WMC locus number : WMC Length of sequence : 426
Last updated : Mer 23 DŽc 1998 at 11:05
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : No overlap between forward and reverse sequences. Multple inserts in this clone, this is the second insert. Trimmed to Ras1 at 5' end and to insert at 3' end. (CA)16 358 to 389 and several small repeats (CA, GCAC). No room for primers.
PCR Comments :



Length of sequence : 426  Consensus sequence is :

..

GTACTTATTC CGCTGATAAG TAGTTCGTTG CTTAGGAATT TCCTCNCCCT 
GAAATTCCTC AGTGACGAAT TTGTAAAAAT CATCTATTCA CCCCCTCTAG 
TCGAAATAAC GCACTTTCAA GCACACACAC ACACATTTTA GCACACACGN 
CNTGCCAACA CACACACACA CCAACNCACA CACTCACNCA CACNCNCACA 
CNCACACNCT CNACCACACA CAAGCACGCN CGCACACGNC AAACACACAG 
CCANCACTAN TCCNCNCCCC CCGAGGGGAT CCTCTANAAT NCAACCTGCC 
AGGCATGCAA GCTTTGGGGT TAATCTTNGG TAAA
(break)
AAAGAGGGTT AAAAAACACA CACACACACA CACACACACA CACACACACT 
CAACCACACA CAAGCACGCA CGCACACGCA AACACACAGC AG

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wmc(2e5) Plate reference : 2e5
WMC locus number : WMC Length of sequence : 136
Last updated : Mer 23 DŽc 1998 at 11:06
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 4 to 124 show homology to 2e9 bases 3 to 123 (121 in longest run, maxScore = 82). No substantial microsatellite. Duplicate, 2e9 has precedence.
PCR Comments :



Length of sequence : 136  Consensus sequence is :

..

GTACATGGTG CTTTGTGTTG TGTAACGTGG AGGCACATAT ACATAATTAG 
AGAGTGAGTG TGCGANATAC ACATGCAGAC ATGGGGAGAG ATGAGGATCC 
AGATTAATGG GTGTTTGTGC GTGTCTGTGT GTGTTT

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wmc(2f5) Plate reference : 2f5
WMC locus number : WMC Length of sequence : 455
Last updated : Mer 23 DŽc 1998 at 11:08
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 455 show homology to 2g5 bases 1 to 455 (455 in longest run, maxScore = 385). No substantial microsatellite. True duplicate with 2g5, 2f5 has priority.
PCR Comments :



Length of sequence : 455  Consensus sequence is :

..

GCCAGCCACC ACTGGCCTCG CCGCCCCGAA ACAACCCCCC ACCGCCGATC 
CGCCGCCGCC CTGACACAAC TCCCCACCAT CGGTCCGCCG CCGCCCCACA 
TATGAAGATA AGGAAGAAAG AGATGTTTAT TTGCTGTCCA AGAAGTTCTC 
TCACATCACT TTTGGCCCAA GAGAAGATAG AGTCCAAGTC TCTCACGTTC 
TGGATTCAGA TTCGGACTGC ACAGACATAC CTGACTCAAA ACGCACACAA 
GTTTTTCATA CGGACTCCGA ATTGGGTGAT TTTTTTTTGT TGGAAACTAG 
CTTTCGTGCA CTTTCCAACC CAATTGGAAT CACCTTCAAA TTCGTCCGGA 
GCGTTGAGTT GTGGACGAAA CAATCTGATG CTGCAGCAGA ATCCGAGTCA 
AACTACAAGT CCAAAGGTGT TACATCACCT CCACTTGGGC CCATTGGCCT 
TGTAC

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wmc(2g5) Plate reference : 2g5
WMC locus number : WMC Length of sequence : 455
Last updated : Mer 23 DŽc 1998 at 11:08
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end ( NB 5' linker corrupted). Bases 1 to 455 show homology to 2f5 bases 1 to 455 (455 in longest run, maxScore = 385). No substantial microsatellite. Duplicate, 2f5 has precedence.
PCR Comments :



Length of sequence : 455  Consensus sequence is :

..

GCCAGCCACC ACTGGCCTCG CCGCCCCGAA ACAACCCCCC ACCGCCGATC 
CGCCGCCGCC CTGACACAAC TCCCCACCAT CGGTCCGCCG CCGCCCCACA 
TATGAAGATA AGGAAGAAAG AGATGTTTAT TTGCTGTCCA AGAAGTTCTC 
TCACATCACT TTTGGCCCAA GAGAAGATAG AGTCCAAGTC TCTCACGTTC 
TGGATTCAGA TTCGGACTGC ACAGACATAC CTGACTCAAA ACGCACACAA 
GTTTTTCATA CGGACTCCGA ATTGGGTGAT TTTTTTTTGT TGGAAACTAG 
CTTTCGTGCA CTTTCCAACC CAATTGGAAT CACCTTCAAA TTCGTCCGGA 
GCGTTGAGTT GTGGACGAAA CAATCTGATG CTGCAGCAGA ATCCGAGTCA 
AACTACAAGT CCAAAGGTGT TACATCACCT CCACTTGGGC CCATTGGCCT 
TGTAC

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wmc(2h5) Plate reference : 2h5
WMC locus number : WMC Length of sequence : 461
Last updated : Lun 30 oct 2000 at 16:59
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 88 to 159 (complement) show homology to 5d6 bases 356 to 427 (72 in longest run, maxScore = 174). (GA)14 251 to 278. Duplicate, 5d6 has precedence. Bases 69 to 158 show homology to TW7a02.2 bases 72 to 161 (90 in longest run, maxScore = 192).
PCR Comments :



Length of sequence : 461  Consensus sequence is :

..

GTACTTCACA ACATGCTCAA ACTCACATAA TCCCAGTCAA ATGAGAATCT 
AAATGCATTT CATAGTTATT ACTTTGTAGG ATGCAACAAA AACAACCAAA 
ACTCTACATC AGCCATTATA GAAGCAACAT TTTGACATAT CCCATTGTGT 
GAATGAAACG TGCAGAGAGA GCTTTTGAAG ATGGAGCAGA TAGGGCAGGA 
AAGATTGTAT GTATGTGGAC GAGAGAGTAG GAGACAAACC TAACGAGACA 
GAGAGAGAGA GAGAGAGAGA GAGAGAGAAA GAGAAAGAGA GAGAGGAAGA 
AGTTAGGTCT GTGAGAAAGA TGGAGACATG TAATATACGT GATTCTATAT 
ACGTGGAAGG AGAAGAGACA ACATGTTTGA TGAGACAGCT AGAAAAACAT 
GGACGAGAGG GGAAGGACCT CATGGGTTGA GGGATTGACA ATTTTGTGCG 
GGGGAGAGAG G

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wmc(2a6) Plate reference : 2a6
WMC locus number : WMC Length of sequence : 281
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 281  Consensus sequence is :

..

TGGCGGCGGC CGACACGTNT TGAGGTTACG CGCATCCTCG TCTCCCTCAT 
CTCCTCCCGC GATCATGCTG CATCCAGATC GGGCGAAAAG ATAAACGAGG 
GGTTAAACGG GAGACATGCG GCGGCTAGGG CTCGCGGAAG GGATTGGTCG 
GCGGCGGCGA ANGGATGGAT CGGCGGCGGC GGCAGCGAAG GCATGGATCT 
GGTGCGAANG GATGGAGGGC GGCGGTGAAT GATCTGCTGT GCGGGTCGTG 
CGTGCTATAC TACTGTGGTT CTCGCTCGTA C

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wmc458(2b6) Plate reference : 2b6
WMC locus number : WMC458 Length of sequence : 265
Last updated : Lun 30 oct 2000 at 16:59
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 160 to 182 (complement) show homology to 1C2 bases 332 to 354 (23 in longest run, maxScore = 40). (CA)18 183 to 218, (GA)4 219 to 226. Bases 165 to 182 show homology to TW7g04 bases 42 to 59 (18 in longest run, maxScore = 34).
PCR Comments : 61C anneal, complex product.
Forward primer details :
gAATTgggcTAAAAcgTggA at 111 length 20 bases, Tm 62
Reverse primer details :
AcTcTTTcgAccTcATggcg at 243 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 152 bases.



Length of sequence : 265  Consensus sequence is :

..

CGTACANAGA TCGAGCGAGT GTGGGTCTTC GTGCGATCGA AAGAGTGAGA 
TGGGTTGGAG AGAGTGATCT AGATAGACAA TGTGCATGAG AGAGTATACA 
ATATGAATAC GAATTGGGCT AAAACGTGGA GATATATCGT TTGTGTCCGA 
GAAAGAGCGA GGTCAATCGA GACATACAAA GACACACACA CACACACACA 
CACACACACA CACACACAGA GAGAGATTGA GCGAGCGTGG CCCGCCATGA 
GGTCGAAAGA GTGGG

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wmc(2c6) Plate reference : 2c6
WMC locus number : WMC Length of sequence : 169
Last updated : Mer 23 DŽc 1998 at 11:21
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)21 121 to 162. No room for primers.
PCR Comments :



Length of sequence : 169  Consensus sequence is :

..

CCCGCGTTGA GGCGGCCAAG CAATACAGGA TGTGTAAATA TTGATGTTTG 
AGCTTAGTCT ATACATCAGA TAGCACATTT GAACTGACAG AACTCAGATG 
TGTCCACGAC AAATGGGTAT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTNTGGTAC

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wmc459(2d6) Plate reference : 2d6
WMC locus number : WMC459 Length of sequence : 328
Last updated : Mer 23 DŽc 1998 at 11:25
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. (CA)14 186 to 213.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
cAgTgATcTTcTgggTgTTTTg at 9 length 22 bases, Tm 61
Reverse primer details :
ATAAcgTgcAgcAcTcATcTcT at 233 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 246 bases.



Length of sequence : 328  Consensus sequence is :

..

GTACCAGTCA GTGATCTTCT GGGTGTTTTG TTTATAAGTA AACGCTTTCA 
AATGAGACAA GAAAACGCAA GCGTCAAATT AGCCAGCCGT TGATGAGACC 
GCTCCACCAG GACGCGACAC ACATACGTAG CGACTGATGA TCGGACTGAG 
GTGGGCATAG GCACCACCCA AGTCTTTCTT GTAAACACAC ACACACACAC 
ACACACACAC ACACGTAGTA GGAAGCAACA GAAGAGATGA GTGCTGCACG 
TTATCGCCGA TCCATGCATG CACGGTGGTG GCGCGAGGAG CAAGCTTTTC 
AATCAAGCAC AGACAGAACA AAAGCGTG

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wmc(2e6) Plate reference : 2e6
WMC locus number : WMC Length of sequence : 572
Last updated : Mer 23 DŽc 1998 at 11:27
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end ( NB 3' linker corrupted). Bases 446 to 529 (complement) show homology to 5b9 bases 85 to 168 (84 in longest run, maxScore = 112). (CA)8 514 to 529. Duplicate, 5b9 has precedence.
PCR Comments :



Length of sequence : 572  Consensus sequence is :

..

GTAGTATGGT GGGCTCTCAT GGCAAAACTG GGTTTGAGGA TATTTGGAAG 
CACAAGTAGT ATCTCTACTT AGTGCAAGGA ATTTGGCTAG CATGTGGGGT 
AAAGACAAGC TCAACATGTT TGGAAGGTCA ATGACAATAT ACTTTAACTG 
AGATGTGAGA AAACATAAAC CATTACGTTG TCTTCCTTGT CCAACATCAC 
TCTTTTAGCA TGTCATACTT AATGAGCGCT TCACAACCAT AAAAGATGTC 
CAAGATAATA TATTTGTATG TGAACCTCTC TTTCCTTATT ACTTCCTATT 
AATTGCCCCC CCACCCCGAC ACACACACCC ACACCCACAC AACCACACAC 
ACACTTCCCA ACACCGAAGG AGTAGGGTGA CACCTCNATC TTGATACTAA 
TCTATCGACT GGCTTCTCTC TCAAACACAC ACACACGCAC ACACACTACC 
CGACACCGAA GGAGTAGGGC GGCACCTCGA TCTTGATAGT AATCTATCTA 
CTCCTCTTTC AAACACACAC ACACACACAT ACACACACAC TCGCTAGTTG 
TGCCTATGTG CCTACTGCGT AC

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wmc(2f6) Plate reference : 2f6
WMC locus number : WMC Length of sequence : 213
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 44 to 52 (complement) show homology to 6h6 bases 582 to 590 (9 in longest run, maxScore = 19). No substantial microsatellite.
PCR Comments :



Length of sequence : 213  Consensus sequence is :

..

GTACGATAAC ACAGAAATAA ACAGGAGCAG GATAAAATGA GTTATACCCT 
CCAGCAGGCC AGGCAAAAGA CGCGGGGGCG GCGGTGGCGG CAACGGCGTC 
TTCAGCGGTC TTCTTGTCGG CTTCGAGTTT CTCAGCGGCG GCTCTCTCGG 
CGTCGGTGGA CATGGTGACG ACGAAGACGA TGCGGACATG GACGAACGGC 
GGCGAGCAAT CGC

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wmc(2g6.1) Plate reference : 2g6.1
WMC locus number : WMC Length of sequence : 247
Last updated : Mer 23 DŽc 1998 at 11:28
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the first insert. Trimmed to Ras1 at 5' end and to insert at 3' end. Bases 75 to 187 (complement) show homology to 7f2 bases 50 to 162 (113 in longest run, maxScore = 98). (CA)26 17 to 68. Duplicate, 7f2 has precedence.
PCR Comments :



Length of sequence : 247  Consensus sequence is :

..

GTACTCTATC GCAAAACACA CACACACACA CACACACACA CACACACACA 
CACACACACA CACACACATC ATCGTCATCA TCATGTATTA AAAACAGAAC 
TACTAATTGG ACTTCAACTG GAATCCGCNN GTGCNTTCAT GGAATANGTT 
GAGCTATGGA CTCCTTGCAT ATATGGACTC GTTTTGCTTC ACCGCAAAAA 
TCAAATNGAT TCCTTTTGCT TAAACGCTGG CCTTGCCTAC GTAGACA

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wmc(2g6.2) Plate reference : 2g6.2
WMC locus number : WMC Length of sequence : 89
Last updated : Mer 23 DŽc 1998 at 11:30
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)5 78 to 88. No room for primers and too small.
PCR Comments :



Length of sequence : 89  Consensus sequence is :

..

TGTGGTGCCT ATCGCTGGCG AGAGTTGGGC GGGCGTATGG ACTCTGGACT 
CCTTTTGCNT ATGTGCCTCC AAAGGATGTG TGTGTGTAC

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wmc(2h6) Plate reference : 2h6
WMC locus number : WMC Length of sequence : 122
Last updated : Mar 22 DŽc 1998 at 10:01
Owner : IGS BioTech Date at Agrogene : 2.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)13 84 to 109. No room for primers.
PCR Comments :



Length of sequence : 122  Consensus sequence is :

..

TCCCTGTACA TACAATGGTA GTGGTTGCGC ATGTAAGGTT GGAATAACGG 
TCGCAAATTA AACAAATCCG TTTTGAAAAA CAACACACAC ACACACACAC 
ACACACACAC TTGCCTCGGT AC

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wmc(21A7) Plate reference : 21A7
WMC locus number : WMC Length of sequence : 359
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 359  Consensus sequence is :

..

CCCAGGGGCA GGCGTAGGAG ACGTAGAGGT GGTACCTGCC CGCCACCGCG 
GGGAAGCGGG CGGAGGCGTC CCTCGACACG AAGCTCCGGA AGGTGGACGG 
CGTCCGGTCG AACGCGCCGG TGTCGGTCAC CTCGTCCAGC GCCGAGCGCG 
CCATCTGACA AAAAGAACAG AGCACCAACC ACTGAGCACA ACTGACTCCG 
TCGACCGAGC ACCTAGCCGA GCCGAGCGCG GGCGTTATTT ACCTTGACGG 
AAGAGGAGGG GGAGGGGGAG GAGGAGTAAC CGGCGGCGGC GGAGAGCGTC 
CTGGCGGCGG CGAGGCTGCG GGAGAGGAGG TGGGCGGATG AGGAGGAGGG 
GACAAGACC

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wmc(21b7) Plate reference : 21b7
WMC locus number : WMC Length of sequence : 241
Last updated : Mer 23 DŽc 1998 at 13:36
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 111 to 174 show homology to 21c5 bases 119 to 182 (64 in longest run, maxScore = 121). (CA)24 194 to 241. No room for primers.
PCR Comments :



Length of sequence : 241  Consensus sequence is :

..

GTAGCCCCAC CGTGAAACTC CTATGGCTAA GTGAAAGTGT TAACGCTATA 
TAGTCCGGTT GCCTAGTTCG ACATGCGATC ACTTCCTTAA TGGACCAAGA 
CGTTGGTTCA AGTGTGATTA CACATATTTT TGCGAACACC CCTGCATTAT 
ATGCATGGGG GCTGAAGCCA ACGACTGCTA ACTTTCAGAA TTGCACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC A

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wmc(21c7) Plate reference : 21c7
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : No sequence 10.11.98.
PCR Comments :
No sequence available for this clone
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wmc(21d7) Plate reference : 21d7
WMC locus number : WMC Length of sequence : 345
Last updated : Mer 23 DŽc 1998 at 13:38
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 181 to 217 (complement) show homology to 10g1 bases 113 to 149 (37 in longest run, maxScore = 56). Bases 151 to 170 show homology to 25b1 bases 257 to 276 (20 in longest run, maxScore = 40). (CA)11 179 to 200. Duplicate, 10g1 has precedence.
PCR Comments :



Length of sequence : 345  Consensus sequence is :

..

AATATTTGCC CGCCTCGAGG GGTTTTATTT TTCATAACAC ACGGTTGTTA 
TCTCCGGACC GTGTGCGATG CACTTTCATC CAAATTTTCA ATAGCCCGGC 
ATTTCACTCC CGCATTTGAC TCCCGCGTAG TAAAAATTTC AGTACCCGCG 
TCATTTCCTC AAACACCCCC TCCACACGCA CACACACACA CACACACACA 
CCAAAACCTC CTCGGGCATG CGCGCGCGCA CACACACAGA CACACACACA 
CCCCTCCCTT GCTCGAAACC CTAGCAAAGT CCCCGCCGCC GCCGCCGCAA 
GGCCTTCATT GTCAACATCT GCCGGTTTGC CCGTGCAGCT TACCC

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wmc460(21e7) Plate reference : 21e7
WMC locus number : WMC460 Length of sequence : 241
Last updated : Mer 23 DŽc 1998 at 13:48
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 59 to 100 show homology to 22b10 bases 170 to 211 (42 in longest run, maxScore = 53). (GA)4 2 to 9, (GA)6 28 to 39, (GA)7 42 to 55, (GA)26 143 to 194.
PCR Comments : 61C anneal, product (400 bases).
Forward primer details :
gAgAAAgggAgAgggAccAAc at 78 length 21 bases, Tm 63
Reverse primer details :
gccTcccTcTccccTTgTAAc at 198 length 21 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 141 bases.



Length of sequence : 241  Consensus sequence is :

..

GGAGAGAGAT GCAGAGAAAG AGAGAGGGAG AGAGAGAGAG TGAGAGAGAG 
AGAGATATAT GGAGAGAAAG AGATGGAGAG AAAGGGAGAG GGACCAACAA 
TAATTTTGTG TTGGATGCGT GGGACAGAAT TGAAGATTGT CGGAGAGAGA 
GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGGTGTT 
ACAAGGGGAG AGGGAGGCGC CAGGGACTTG GGTACGGCTG C

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wmc(21f7) Plate reference : 21f7
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : No sequence 10.11.98.
PCR Comments :
No sequence available for this clone
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wmc461(21g7) Plate reference : 21g7
WMC locus number : WMC461 Length of sequence : 269
Last updated : Mer 23 DŽc 1998 at 16:13
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 5' linker corrupted.) . Bases 84 to 103 (complement) show homology to 5b8 bases 299 to 318 (20 in longest run, maxScore = 73). (CA)5 20 to 29, (CA)9 102 to 119, (CA)7 197 to 210.
PCR Comments : 61C anneal, product (180-200).
Forward primer details :
cccgAcAccAAAggAgTAgg at 33 length 20 bases, Tm 64
Reverse primer details :
cAcAgAAgcAcAAcAAgcgA at 212 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 199 bases.



Length of sequence : 269  Consensus sequence is :

..

CTACCCCCCC CCACACACCC ACACACACAC TTCCCGACAC CAAAGGAGTA 
GGGTGACACC TTGATCTTGA TACTAATCTA TCTACTGGCT TCTCTCTCAA 
ACACACACAC ACACACACAC TACCCGACAT CGAAGGGTAG GGTGGCACCT 
CGATCTTGAT AGTAATCTAT CTACTGGCTT CTCTCTCAAA CACAAGCACA 
CACACACACA CTCGCTTGTT GTGCTTCTGT GCCTACCGCG CGCACACTCT 
TGATACGTCT TTCTCTCCC

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wmc462(21h7) Plate reference : 21h7
WMC locus number : WMC462 Length of sequence : 318
Last updated : Mer 23 DŽc 1998 at 16:19
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)19 260 to 297.
PCR Comments : 51C anneal, product (circa 211 bases).
Forward primer details :
gTgTTTTAgcAgTTAcTccgT at 108 length 21 bases, Tm 56
Reverse primer details :
AATAATggTTTcTAAggTggg at 298 length 21 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 211 bases.



Length of sequence : 318  Consensus sequence is :

..

CCACACTTAA CACTGTACTA GTAGTACGTA GTCGGTATTT TCTTTTAGAG 
AAGAGGCCCT CTTAGGTACG TAGTCAGTAT TCCTTTCTTC CCTTCTGAAG 
TACTGCTGTG TTTTAGCAGT TACTCCGTGT TTTAGCAAAG GCGTGCGTAC 
GTGTTTTAGC AGCATACGGC GATCGAGCAT GGGTTGCTAG CTAGCTAGCT 
TAGCTTAGCT AGTGTGCACA TGTGAGATGT GACGAGAATT GGCAGATCCA 
GAAACCTGTC ACACACACAC ACACACACAC ACACACACAC ACACACACCC 
ACCTTAGAAA CCATTATT

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wmc463(21a8) Plate reference : 21a8
WMC locus number : WMC463 Length of sequence : 275
Last updated : Mer 23 DŽc 1998 at 16:23
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)26 184 to 235.
PCR Comments : 51C anneal, polymorphic on acrylamide (115, 152-156 bases).
Forward primer details :
gATTgTATAgTcggTTAccccT at 121 length 22 bases, Tm 58
Reverse primer details :
ATTAgTgcccTccATAATTgTg at 251 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 152 bases.



Length of sequence : 275  Consensus sequence is :

..

GCACATGTGA TCATGAATCC ATTGTTAATT GTGCATGTAA GAGTATATGG 
TGTAGCACAT GTATCTCAAC CTCCTAGCCA ATCCGCCCAT GGTTTAAAAA 
GTATATATCT GTATGGGATA GATTGTATAG TCGGTTACCC CTTATATAAT 
TATGTGTATT ATAGCTTATC TTAGCTTTAA ACCGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTTTGGA TCCATCATTG 
CACAATTATG GAGGGCACTA ATATT

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wmc(21b8) Plate reference : 21b8
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : No sequence 10.11.98.
PCR Comments :
No sequence available for this clone
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wmc(21c8) Plate reference : 21c8
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : No sequence 10.11.98.
PCR Comments :
No sequence available for this clone
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wmc(21d8) Plate reference : 21d8
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : No sequence 10.11.98.
PCR Comments :
No sequence available for this clone
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wmc(21e8) Plate reference : 21e8
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : No sequence 10.11.98.
PCR Comments :
No sequence available for this clone
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wmc(21f8) Plate reference : 21f8
WMC locus number : WMC Length of sequence : 468
Last updated : Mer 23 DŽc 1998 at 16:29
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends ( NB 5' linker corrupted). Bases 377 to 467 (complement) show homology to 11h12 bases 1 to 91 (91 in longest run, maxScore = 155). (GC)5 52 to 61, (CA)6 63 to 74. Duplicate, 11h12 has precedence.
PCR Comments :



Length of sequence : 468  Consensus sequence is :

..

GCACAATCCT TGATATTATG AAATATGTCT TCGTCCTTTT ATCTCGATCA 
TGCGCGCGCG CACACACACA CACATCCCAC GTATACATGT ATACCTCTCA 
CACATGCACG TTCAAGGTCT CTATGTCTCC ATACGTTTAA TTACCCTCAA 
TCCTAACGCC ACATCGAATC GTTCTCCTCT TTTGTGCACA TAACTTCCGC 
CAGGATGGGT AAAACACACA CTCACACTTA ACAATCTCCT TCTAGCTACC 
CCCCCTGCCT CTCACTCTTC CCCTATATCC CCGAAACCAA TAAGACCATC 
CCTTTGTTTA ATTCCCGCCT ACATGCGCCA TCGATATATA GTAGTCTTCC 
TCGGTGTCTC TCGAACAAAC ACGTTCTCTC GATGTCGACT CCTCTCACGC 
GCACACACCT TCTCTTCCCT CTCTACGGGC ATTTTCTCTC TCGCACCCCA 
TCGTTGGTGG CCTTTGCC

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wmc(21g8) Plate reference : 21g8
WMC locus number : WMC Length of sequence : 340
Last updated : Mer 23 DŽc 1998 at 16:34
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 32 to 74 show homology to 6a11 bases 232 to 274 (43 in longest run, maxScore = 38). (GT)5 1 to 10, (GA)10 11 to 30, (GT)6 54 to 65, (GT)7125 to 138, (GT)5 140 to 150, (GA)7 151 to 164, (GT)4 181 to 188. No room for primers.
PCR Comments :



Length of sequence : 340  Consensus sequence is :

..

GTGTGTGTGT GAGAGAGAGA GAGAGAGAGA GGAGAGAGAT GGAGTCTTAG 
TTTGTGTGTG TGTGTGAGAG AGAGGGAGAG AGATGGAGTC TTATTGTGTG 
GGGGGGGGGG GCGTGCATGC TTGCGTGTGT GTGTGTGTTT GTGTGTGTGT 
GAGAGAGAGA GAGATGGAGT CTTAGTGTGG GTGTGTGTCA CTGAACTATC 
AAGTAGTCAT CAACACGCGA CTGTCAGGCA TTCACAATTT CTGCAGTGCC 
GCCGCGGGTC GTACACCTAG CGGTGCTTCC AGGACGTAAT TCTTCTGTGC 
AGCAATAAGG ACAATCCTCA AGTTACGGAC CCAGTCCGTG

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wmc(21h8) Plate reference : 21h8
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:25
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : No sequence 10.11.98.
PCR Comments :
No sequence available for this clone
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wmc(21a9) Plate reference : 21a9
WMC locus number : WMC Length of sequence : 263
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 5' linker corrupted). Bases 95 to 138 show homology to 12a9 bases 404 to 447 (44 in longest run, maxScore = 113). No substantial microsatellite.
PCR Comments :



Length of sequence : 263  Consensus sequence is :

..

GGATATATAG AGACCACTCT CGCTTGTGCT TCTCTGCACC GCAGCATGCA 
CAACACCTCA ACCTTCAAAG AGGGCATCGA GGAGATCGAA GACCGTGACC 
ACACTGCACT AGAGAATGCG GGGCCATTTG GAAGCAGGAT GATGTGATGA 
CGGCTGACTG ACTCCTCTCC CCTCTCTCTC ACGCACAAAA TTACCAATCC 
CTCTCTCTCC CGGAGAAAAT TATCAATCCC TCAACCCACG AGATCCTCCC 
CCTCTCGTCC ATG

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wmc(21b9) Plate reference : 21b9
WMC locus number : WMC Length of sequence : 167
Last updated : Mer 23 DŽc 1998 at 16:38
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 3 to 78 show homology to 5e3 bases 150 to 225 (76 in longest run, maxScore = 42). (GT)12 55 to 78, (GT)7 101 to 114, (GT)7 151 to 164. No room for primers.
PCR Comments :



Length of sequence : 167  Consensus sequence is :

..

GTGAGAGTGG TCGCCGTATA ACCTTGGATG GGGATGAATT GTGTGCTCGG 
GGGAGTGTGT GTGTGTGTGT GTGTGTGTTG TGTTGCGTGT CTCTCTCTCT 
GTGTGTGTGT GTGTTGTGCT GCGTGTCTCT CTCTCTCAGT GTGTGTGTTT 
GTGTGTGTGT GTGTCAG

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wmc(21c9) Plate reference : 21c9
WMC locus number : WMC Length of sequence : 314
Last updated : Mer 23 DŽc 1998 at 16:44
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at both ends of insert. Bases 2 to 23 (complement) show homology to 17f7 bases 326 to 347 (22 in longest run, maxScore = 24). (CA)5 26 to 35, (CA)5 64 to 73, (CA)6 81 to 92, (CA)16 125 to 156. Too difficult to make primers. Microsatellites embedded in minisatellite like repeat structure.
PCR Comments :



Length of sequence : 314  Consensus sequence is :

..

AATATTCACC ATCAAGCCAC ACAGTCACAC ACACAGTCAA GCCACACGCA 
CACAGAGTCA AGCCACACAC ACAATCAAGC CACACACACA CAAAGTCAAG 
CCACACACAT ATTCACCATC AAGTCACACA CACACACACA CACACACACA 
CACACAGAAA GAATGGAGCG CTTGCACACA AGAATAGGGG CACACAGAGA 
AACCTAATGC ACGACCGAAG TAAACTCAAA ATGCTATATA GAAATTCCAC 
AACTTGAATG AGTAAACTGA TTTCAAGTTG GCAAGAGTGG GTGCCTCTCA 
ATAGTTACAA TATT

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wmc(21d9) Plate reference : 21d9
WMC locus number : WMC Length of sequence : 141
Last updated : Lun 28 DŽc 1998 at 9:56
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 87 to 135 (complement) show homology to 4c12 bases 73 to 121 (49 in longest run, maxScore = 45). (GT)5 13 to 22, (GT)7 28 to 41, (GA)8 42 to 58, (GA)8 85 to 100. No room for primers.
PCR Comments :



Length of sequence : 141  Consensus sequence is :

..

GATGGAGTCT TAGTGTGTGT GTGGGGGGTG TGTGTGTGTG TGAGAGAGAG 
GAGAGAGATA TGGAGTCTTA GTTTGTGTGT GTGTGAGAGA GAGAGAGAGA 
TGGAGTCTTA GTGTGTGTGG GGGGGTGCGT GCGTGCGTGT G

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wmc(21e9) Plate reference : 21e9
WMC locus number : WMC Length of sequence : 134
Last updated : Lun 28 DŽc 1998 at 10:07
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends ( NB 5' linlker corrupted). Bases 46 to 72 (complement) show homology to 2f3 bases 308 to 334 (27 in longest run, maxScore = 28). (CA)6 38 to 49, (CT)6 62 to 73, (CA)8 74 to 89, (CA)7 92 to 105. Too difficult to make primers.
PCR Comments :



Length of sequence : 134  Consensus sequence is :

..

AGCAAGGGAA CACAAACACA CACACGCACA CCCCCCCCAC ACACACACAC 
TAAGACTCCA TCTCTCTCTC TCTCACACAC ACACACACAA ACACACACAC 
ACACATGCAC GCATGCACAC CCCCCCCCCC CACA

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wmc(21f9) Plate reference : 21f9
WMC locus number : WMC Length of sequence : 182
Last updated : Jeu 31 DŽc 1998 at 9:49
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 64 to 115 (complement) show homology to 7b1 bases 51 to 102 (52 in longest run, maxScore = 70). Bases 98 to 126 show homology to 25d9 bases 76 to 104 (complement) (29 in longest run, maxScore = 70). (GT)7 117 to 130, (GT)5 133 to 142, (GT)6 145 to 156, (GT)7 163 to 176. No room for primers. Duplicate with 25d9, 21f9 has priority.
PCR Comments :



Length of sequence : 182  Consensus sequence is :

..

AATATTTAAC AACTTTTCCA TCTAATTCAT CTAAAATTAA CATTCTAAGA 
TTCTTATCTA CGTAATTCAT CTAACATTAA TCTAAACAAC AGAAAACAGA 
AAATAAGTAA AAAAATGTGT GTGTGTGTGT ATGTGTGTGT GTATGTGTGT 
GTGTGTATGT ATGTGTGTGT GTGTGTACGA GC

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wmc(21g9) Plate reference : 21g9
WMC locus number : WMC Length of sequence : 402
Last updated : Lun 30 oct 2000 at 16:59
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 178 to 222 (complement) show homology to 6b10 bases 187 to 231 (45 in longest run, maxScore = 127). No substantial microsatellite. Bases 189 to 217 show homology to TW7h10.1 bases 162 to 190 (complement) (29 in longest run, maxScore = 66).
PCR Comments :



Length of sequence : 402  Consensus sequence is :

..

AATATTTGGG AAGAGGCCTG GGGTCTTGCT CTATAAATAG GACTAGCCAC 
CATAGCATAG AGGCATCTCG AATCGCCCCC CCCCCCAAAA GGGACAACAA 
CACCATAAGA ACAGACCCTC GCGAGGCTGT TCTTCCCTTT GTATTGTTCA 
TCATCAGCCC CAGAGGCAAT CCACCACACC ACACACTGGA GTAGGGTATT 
ACACCACAAT GGTGGCCTGA ACTAGTATAA ATCTCGTGTC CCTTGAGTTG 
TTCTTCATGT AGTTTAGATC TTAGCGAGGC GGTGAGACAT GAGTAGGCTG 
GAGGTGTGAT CTCCGTGTGC ACCCCAGTGT TCGAACCTCA AGGGTCTGCC 
GGAACACGAA ATCCGACATT TGGCGCGCCA GGTAGGGGTG CGCCAGAGCT 
CT

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wmc(21h9) Plate reference : 21h9
WMC locus number : WMC Length of sequence : 227
Last updated : Lun 28 DŽc 1998 at 10:12
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 132 to 173 (complement) show homology to 21f6 bases 6 to 47 (42 in longest run, maxScore = 64). (GT)16 38 to 69. Duplicate, 21f6 has precedence.
PCR Comments :



Length of sequence : 227  Consensus sequence is :

..

AGCCGTGTGA TTTGCTCTGT TTACAGAAGA ATAACATGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTA TAATGGAAAT AAACATCCAA TTACATAAAT 
GACTATAGAG ATCATTCACA CACACCTCAA CAAACAATTG CATGCATTCT 
AAAGTAGGAG AAACGATCGT CTAGTCATCT ACTTCTTGTC AAGCTCCCGC 
CACTGCTCTG TGGCACTTCC TCATATT

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wmc464(21a10) Plate reference : 21a10
WMC locus number : WMC464 Length of sequence : 245
Last updated : Lun 28 DŽc 1998 at 10:47
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 126 to 156 show homology to 20f5 bases 154 to 184 (31 in longest run, maxScore = 36). (CA)13 126 to 151, (GT)7 223 to 236.
PCR Comments : tested 61C anneal, no product. 51C anneal, product (complex).
Forward primer details :
gggAcgggAATATgcATAgTgA at 50 length 22 bases, Tm 65
Reverse primer details :
AcATAgAggAAcATcggTcgcT at 203 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 175 bases.



Length of sequence : 245  Consensus sequence is :

..

GTTATGCCGG TGGGAGTAGA TAGGATGCTA AAGTAGTGCT ACGCAAAAAG 
GGACGGGAAT ATGCATAGTG ATGGGCATTA TCCATCACAT ACATGCAATC 
TTTTTCTTGA GATCGGTGTC CCATCCACAC ACACACACAC ACACACACAC 
ACCTCCTCCT CTTCATCATA TCGGGCATTC TTTATCTCTC ATGTGCATGC 
GCAGCGACCG ATGTTCCTCT ATGTGTGTGT GTGTGTATAG CTAGG

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wmc(21b10) Plate reference : 21b10
WMC locus number : WMC Length of sequence : 383
Last updated : Lun 30 oct 2000 at 16:59
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Sequence was identified in Dupont file as 21b1 - unsure if this is 21b10 or 21b11.Trimmed to insert at both ends. Bases 96 to 122 (complement) show homology to 21a5 bases 215 to 241 (27 in longest run, maxScore = 62). (GA)7 72 to 85, (GA)8 153 to 168, (GA)5 80 to 89, (GA)8 171 to 186, (GA)17 250 to 283, (GA)9 347 to 364. Too difficult to make primers. Microsatellites embedded in minisatellite repeat like structure. Bases 239 to 265 show homology to TW3A01.1 bases 493 to 519 (27 in longest run, maxScore = 28). Bases 207 to 273 show homology to TW6g05 bases 380 to 446 (67 in longest run, maxScore = 51).
PCR Comments :



Length of sequence : 383  Consensus sequence is :

..

GAGAGAGATG GAGGGAGAGG GACCAACAAG GTTTTTGTGC TGGATGCGTG 
CGACAGAACT GAAGATTGTC AGAGAGAGAG AGAGACATAG AGAGAGAGAG 
GGACCAACAA GGTTTGTGTG TTGGATGCGG GCGACAGAAC TGAAGATTGT 
CAGAGAGAGA GAGAGAGACA GAGAGAGAGA GAGAGATGGA GGGAGAGGGA 
CCAACGAGGT TTGTGTGTTG GATGCGTGCG ACAGAACTGA AGATTGTCAG 
AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGATGGAGGG AGAGGGACCA 
ACGAGGTTTG TGTGTTGGAT GCGTGCGACA GAACTGAAGA TTATCAGAGA 
GAGAGAGAGA GAGAGGGACC AACAAGGTTT GTG

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wmc465(21c10) Plate reference : 21c10
WMC locus number : WMC465 Length of sequence : 219
Last updated : Lun 28 DŽc 1998 at 11:11
Owner : Dupont Date at Agrogene : 10.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 68 to 108 show homology to 1C4 bases 239 to 279 (41 in longest run, maxScore = 80). (GT)13 155 to 180.
PCR Comments : 61C anneal, product ( very stuttery 100-120 bases).
Forward primer details :
gccAccgATgTAAgTggATgT at 103 length 21 bases, Tm 64
Reverse primer details :
AAggTgTgTgcgATgggATT at 196 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 113 bases.



Length of sequence : 219  Consensus sequence is :

..

CTTTATTGTT GGTGTGTGGA AATGTCATGT GTGCAGTAGC CACCTATGTA 
ATTATGCATG TAATAGATTA CTTTGGTGTG TGCAAATGCC ATGGTTGTAG 
TAGCCACCGA TGTAAGTGGA TGTTTAATTT TGTTATGCAA GCATGTCCTT 
ATAAGTGTGT GTGTGTGTGT GTGTGTGTGT TGTTGTCCTG TATGCAATCC 
CATCGCACAC ACCTTGATC

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