Wheat microsatellite WMC 25/06/01

wmc(25f2) Plate reference : 25f2
WMC locus number : WMC Length of sequence : 56
Last updated : Mar 29 DŽc 1998 at 9:31
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end 3' end not located.(GT)24 9 to 56. No room for primers.
PCR Comments :



Length of sequence : 56  Consensus sequence is :

..

CTGTTCTTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGT

Go back to index

wmc(25g2) Plate reference : 25g2
WMC locus number : WMC Length of sequence : 306
Last updated : Mar 29 DŽc 1998 at 9:32
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)18 1 to 36. No room for primers.
PCR Comments :



Length of sequence : 306  Consensus sequence is :

..

CACACACACA CACACACACA CACACACACA CACACATTAA TGGCCGCACA 
GGAGATATTT CAATACTTGA ACGCACAAGT ATAAAAAGCC CTTATATTAT 
AAATATGGTT TTACAAATCA TACATGTCAC TTGAACATAA CGTTCTTCGA 
GCACTGCGAC TCTATTAAAC GAGCGCCCTG CAAGACTTCC TCAAAATAGT 
ACTCGGCGGG TAATCGGCTT CTGTCCGAAT CCCGGGATGC AACGTCGGTG 
GCATCCATCT CTGCCCAGTA TGTTTTGACA TGGACGAGAG CCATCCGTGC 
ACCCTC

Go back to index

wmc(25h2) Plate reference : 25h2
WMC locus number : WMC Length of sequence : 386
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Forward sequence only. Trimmed to insert at both ends. Bases 1 to 45 (complement) show homology to 3g11 bases 114 to 158 (45 in longest run, maxScore = 45). No substantial microsatellite.
PCR Comments :



Length of sequence : 386  Consensus sequence is :

..

CCCGCGGGTG CACAAATTGT AACATACGTG TCTGCTTAAG TGGGCATCAC 
GTAACTGGTG TGTATGTGTG AGAGAGATTC TAAGAGGCAG AGTGCGTGTG 
TGCGACTCTA CTCTTCAGAC ATGTACTCAA CCTTTTGCAT CGGGATATAA 
GTATAATGGT ATTTACTTTA GCTAGTGATT TACGTGGCCA TGTTAACGTG 
GTTGTGTAAA AAAATGCCAC TTCCTGCTTG TGATTTGCGT TATATAATTA 
CAAGTAAGAT GCTCGTGCAT TGCACGGAAC ATCAATATGC ATTTTTTTAC 
AAATCACCTG TTGTGATTGA CCCATGCAGG AGTAATCCCA TATGTTAAAA 
CTAATGATAT CTCGAGAATT TTATCGGAAA ACTAGT

Go back to index

wmc(25a3) Plate reference : 25a3
WMC locus number : WMC Length of sequence : 76
Last updated : Mar 29 DŽc 1998 at 9:38
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end (NB 5' linker corrupted). Bases 1 to 69 (complement) show homology to 12g8.1 bases 80 to 148 (69 in longest run, maxScore = 57). No substantial microsatellite. Duplicate, 12g8.1 has precedence.
PCR Comments :



Length of sequence : 76  Consensus sequence is :

..

TCCTGTAGTG AAATTGTAAC AATGCATGAA AATAAAGTTC AGATGTGTAG 
CCCAACGATA GATTGTATGG AATATT

Go back to index

wmc(25b3) Plate reference : 25b3
WMC locus number : WMC Length of sequence : 405
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 30 (complement) show homology to 3a9 bases 422 to 451 (30 in longest run, maxScore = 51). No substantial microsatellite.
PCR Comments :



Length of sequence : 405  Consensus sequence is :

..

GCCGCCTCCG AAGTGGGTGC GGAACTTGAA GACGAAGATG CAGTTGGGGT 
CCTTCACCTC GTACACCTTG GCGAGCCTCT CCTTCAGCTC CGCCTGAGCA 
CAAGCACACG AACGCTTCAG AATAGCCGAC GAAATGCAAC GGAGCTAAGG 
AACGAGAGGA AGGGGGCGAG ATCTCCAACC TTGGAGACGT TGGCGCGGCC 
CGGGTGGATG ACCTCGAGCA CGAACTGCTT ACGGGCGAGC AGGCGGTTGG 
TCATGAACTT GCGGGTGCGG AGGGTGACCG CCGTCGTGGC CTTGGCGTCT 
GCCATGGCTG ATTCGACCTC GCTTCGCGGT GGGCTGCCTG CGGAGGTGGC 
GGCAGCGGCG GCGGCGGCGC GAGAGGGAGG AAAGCGGAGG TAGGGAAGAG 
GGGAG

Go back to index

wmc(25c3) Plate reference : 25c3
WMC locus number : WMC Length of sequence : 172
Last updated : Mar 29 DŽc 1998 at 9:44
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. (GT)35 16 to 85. No room for primers.
PCR Comments :



Length of sequence : 172  Consensus sequence is :

..

ATTATTTCAC AACTTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTTCCGT AGATGATAGC 
CCGCGCGTTG CTGCGGAAAT TTCGTAGCGT TGTAGAAGTA TCGAAGGAAG 
ATTCATCGAG TGGCAGATAG TG

Go back to index

wmc(25d3) Plate reference : 25d3
WMC locus number : WMC Length of sequence : 62
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (5' linker corrupted). No substantial microsatellite.
PCR Comments :



Length of sequence : 62  Consensus sequence is :

..

ATGGCGAGGA GTATAGCGGC GAGCTCAAGG GCAGTGGGAT GATGCTACGG 
CGGTCAGCAT GC

Go back to index

wmc474(25e3) Plate reference : 25e3
WMC locus number : WMC474 Length of sequence : 154
Last updated : Mar 29 DŽc 1998 at 13:55
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)20 53 to 92.
PCR Comments : tested 61C anneal, no product. 51C anneal, product.
Forward primer details :
ATgcTATTAAAcTAgcATgTgTcg at 3 length 24 bases, Tm 60
Reverse primer details :
AgTggAAAcATcATTccTggTA at 103 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 120 bases.



Length of sequence : 154  Consensus sequence is :

..

GTATGCTATT AAACTAGCAT GTGTCGTGTA TGTATACGGT GCGTGTACTC 
ATGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGAGGCACA 
CTTACCAGGA ATGATGTTTC CACTTTTGTC GGTCTCTTTT CCATGCACCT 
TAGG

Go back to index

wmc475(25f3) Plate reference : 25f3
WMC locus number : WMC475 Length of sequence : 385
Last updated : Mar 29 DŽc 1998 at 14:01
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)21 292 to 333.
PCR Comments : 61C anneal, product (1 sample failed in PCR).
Forward primer details :
AAcAcATTTTcTgTcTTTcgcc at 206 length 22 bases, Tm 62
Reverse primer details :
TgTAgTTATgcccAAccTTTcc at 338 length 22 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 154 bases.



Length of sequence : 385  Consensus sequence is :

..

ACCATAATCA AATCCTTTTT TGCCCTTCTA AATTAGTTGG CTCGATTTCG 
TTGATAATTG AGTATGATTG GCAGTATATA CTCCAGTGTC TTCGTTGTTC 
TGTTTTATAA TCCAATTGCC AGGATGTTTG CTATATTTTT AGTATGTACG 
TAGATCGTAG GGACATTACT GTAGTACAAA TGTCTTCTTT ACAGGTTATG 
CAGTCAACAC ATTTTCTGTC TTTCGCCCTG TTCGTTTGCT GAAGCTCGTG 
CTCATGTTGT AATTGTGGCA TGTAATCAGT CCCTGATCCA TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGACCGGA AAGGTTGGGC 
ATAACTACAT TGTTAGGGAG AAGATGAAGA ATATT

Go back to index

wmc(25g3) Plate reference : 25g3
WMC locus number : WMC Length of sequence : 331
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : No substantial microsatellite.
PCR Comments :



Length of sequence : 331  Consensus sequence is :

..

GGCAGCTTCC CGAGCGCAGC CCTTTAAAAA GTGAAGCGGT ATTTCTCATT 
GCCCTGTTTG TCGCTTGTAT TTACGTAGGG TTTCGTGCTG CACGTAGGGT 
TTCCGCCGCC ACCGTCCTGC CTTCCCCCGC CACCGTCCAG GCTCCCTCCG 
CCAATCACCA CCAGCCGCCG CCACCGGCGC CGCAGTCGTC GCTCGCATGG 
AGTCTCCTGG GTAGCCGACG CAGCTACAGA TCTGCTCGGA CGCCCCGCTC 
CTGGAGGTCT GTCCATTGTT CTTGTACTCG AGGGACGCCC CACTCCAGCT 
CTCCACTACA ACTACGACAT AACTCAATAT T

Go back to index

wmc476(25h3) Plate reference : 25h3
WMC locus number : WMC476 Length of sequence : 308
Last updated : Mar 29 DŽc 1998 at 14:30
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Forward sequence only. Trimmed to insert at both ends. Bases 202 to 235 show homology to 9e5 bases 183 to 216 (34 in longest run, maxScore = 83). Homology to one side of microsatellite. Reverse primer three mismatches to 9e5. (GT)7 118 to 131, (GT)25 134 to 183.
PCR Comments : tested 61C anneal, no product. tested 51C anneal, no product.
Forward primer details :
TAccAAccAcAccTgcgAgT at 90 length 20 bases, Tm 63
Reverse primer details :
cTAgATgAAccTTcgTgcgg at 270 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 200 bases.



Length of sequence : 308  Consensus sequence is :

..

CAACCCCCTC CCCCATTTGC TTCCGATCAA ATGTAGACCA TGTGCAGACC 
CACAGATGTG TCTTTTGGCG TTAGATTTGT GAGACTACTT ACCAACCACA 
CCTGCGAGTG GCCCGATGTG TGTGTGTGTG TCTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGAAAACG ATGTGTTGTG 
GTATTTAAAT AACCAAATCA CAAATTTGAT GTGGCACATG TGCATCGCAA 
ATTAACCGGG CATCCCCGAC CGCACGAAGG TTCATCTAGT ATGAAGTAGT 
AATGTGCC

Go back to index

wmc(25a4) Plate reference : 25a4
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : No sequence 17.11.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc477(25b4) Plate reference : 25b4
WMC locus number : WMC477 Length of sequence : 189
Last updated : Mar 29 DŽc 1998 at 14:44
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)16 118 to 149.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
cgTcgAAAAccgTAcAcTcTcc at 10 length 22 bases, Tm 65
Reverse primer details :
gcgAAAcAgAATAgcccTgATg at 155 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 167 bases.



Length of sequence : 189  Consensus sequence is :

..

TAGTGCGTGC GTCGAAAACC GTACACTCTC CCCACACAGA GCGAGATCGG 
TTCATGATGT GTCGTTGTAC TAGCCACTTC GACTCGATGG GAGGGATTCA 
TGCGTACACA TGCATGCGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTA 
TACACATCAG GGCTATTCTG TTTCGCGAAA CAGAATATT

Go back to index

wmc(25c4) Plate reference : 25c4
WMC locus number : WMC Length of sequence : 157
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 157  Consensus sequence is :

..

CAGAACCTGA GCGAAACGCA CTCACACCCC TCTCCGCCTC CGCCTCCGCC 
GCGCTAGGGT TTTGTCTCCA CCTAACCACA CTCACAGCCG CCTCCGCCTC 
CGCCGCATTT CGGCCGCGCT CTCGCCAAAA TCGCCTCCCC GCATCACCTG 
TTCCTTC

Go back to index

wmc(25d4) Plate reference : 25d4
WMC locus number : WMC Length of sequence : 199
Last updated : Mar 29 DŽc 1998 at 14:48
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 108 to 138 show homology to 6d6 bases 74 to 104 (31 in longest run, maxScore = 24). Bases 151 to 171 show homology to 20d10 bases 128 to 148 (21 in longest run, maxScore = 26). (CA)10 9 to 28, (CA)6 58 to 69, (CA)6 73 to 84. No room for primers.
PCR Comments :



Length of sequence : 199  Consensus sequence is :

..

GCCATGTCCA CACACACACA CACACACACT ACACACACAC ACTACACACA 
CACACTACAC ACACACACAC TACACACACA CACATTATTA GTTTGTTTGT 
TATAAATTTT TTCTGTTTTT TAGTTATAGA AATGTTAGGA AATTATTCTG 
TTTTTTAGTT ATATAAATAT TGGTTTGATT TTTATCTCTG TCCAATATT

Go back to index

wmc478(25e4.1) Plate reference : 25e4.1
WMC locus number : WMC478 Length of sequence : 282
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at both ends. Bases 101 to 128 show homology to 21h4 bases 153 to 180 (28 in longest run, maxScore = 110). Bases 173 to 222 show homology to 25a6 bases 207 to 256 (50 in longest run, maxScore = 88). Bases 194 to 228 show homology to 25b10 bases 165 to 199 (complement) (35 in longest run, maxScore = 111). (GT)6 23 to 34, (GT)11 43 to 64, (GT)5 71 to 80, (GT)6 87 to 98, (GT)6 101 to 112. Duplicate with 25a6, 25e4.1 has priority. These primers may cause problems due to the number of other copies of the sequeence 5' to the microsatellite i the genome. Bases 245 to 282 show homology to 22a5 bases 137 to 174 (complement) (38 in longest run, maxScore = 114).
PCR Comments : tested 61C anneal, no product. 51C anneal, smear.
Forward primer details :
TgAATTgTgTgcTcgggg at 3 length 18 bases, Tm 64
Reverse primer details :
cgcgcAATcTcTTcgTgA at 155 length 18 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 170 bases.



Length of sequence : 282  Consensus sequence is :

..

GATGAATTGT GTGCTCGGGG GAGTGTGTGT GTGTTGTGCT GCGTGTGTGT 
GTGTGTGTGT GTGTGCATGC GTGTGTGTGT CTCTCTGTGT GTGTGTGTGA 
GTGTGTGTGT GTCAGATATT GAGCGAAAAC AAACCTAAGG AGAGAAATGG 
GTATTCACGA AGAGATTGCG CGGGCCTTGA GGTGGGCAGG GGCCGAGTGA 
GGGTGTCAAA ATGTGCGCGT TGATGCATAT GTTGCATGCG ATCGTGCGAG 
GGACGGACGA ATCGAGAGAG ATGGTTGTTG TG

Go back to index

wmc479(25e4.2) Plate reference : 25e4.2
WMC locus number : WMC479 Length of sequence : 453
Last updated : Mar 29 DŽc 1998 at 15:50
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)16 259 to 290.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
gAccTAAgcccAgTgTcATcAg at 162 length 22 bases, Tm 63
Reverse primer details :
AgAcTcTTggcTTTggATAcgg at 336 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 196 bases.



Length of sequence : 453  Consensus sequence is :

..

GAGGGGCGTT TGGTTGGGCT TTTACTTGGT TTTTTTCACT TTGGCTTATA 
AGCCAAAAAA AAACACCTAT TTAGATGTTT TTTTTTGGCT TATAAAGTGC 
CTTATCTGTT TTTTGGCTTA TAAGCCAAAG TGAAAAAAGC CCAATGTAGT 
TAAGAATTAT AGACCTAAGC CCAGTGTCAT CAGCTCCGCT CATGGACTCT 
CTCTTGGCTG CTCCAAGAAG AAGATGGTAA CGAACAAGCC GGACATAAAA 
GGGTACTTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GAAGACGGAA 
GAATCTGCAA GACTTGTTTC TTCCAGGCAC ACAACCCGTA TCCAAAGCCA 
AGAGTCTAGA GTAGGTCGGG CCTACCCCTA AGCCTCTCCC TTTTCTCTTC 
ATATTATTTT GTGAAGTGCA ACTAAAATTT AAAGTAGCTG CAACGAAAAT 
ATT

Go back to index

wmc(25e4.3) Plate reference : 25e4.3
WMC locus number : WMC Length of sequence : 253
Last updated : Mar 30 mai 2000 at 9:54
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the third insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)35 87 to 156. Too difficult to make primers.
PCR Comments :



Length of sequence : 253  Consensus sequence is :

..

GCGCAGACCC TGCCAAAAAA CGAACAAACA CATAGGCCGG CCCAACTAGC 
TCCATTCCGG TATGGGGAAG GTTTCCCAGG CCATTTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTCCCA TTTTCATTCT TTCCTTCTTT TTCATATTTT TGTTTTCTTT 
TTTTCTTTTT TCATGCTCAT GTTCATTTTT CTTTCCTTTT TAGTTTGAAT 
ATT

Go back to index

wmc(25f4) Plate reference : 25f4
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : No sequence 17.11.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(25g4) Plate reference : 25g4
WMC locus number : WMC Length of sequence : 28
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). No substantial microsatellite.
PCR Comments :



Length of sequence : 28  Consensus sequence is :

..

TCGGTCTTCG CACACATTTC TGATTACA

Go back to index

wmc(25h4) Plate reference : 25h4
WMC locus number : WMC Length of sequence : 204
Last updated : Jeu 11 FŽv 1999 at 15:06
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. (GT)26 153 to 204. No room for primers. Bases 3 to 111 show homology to 22f5 bases 73 to 181 (complement) (109 in longest run, maxScore = 117). Duplicate with 22f5, 25h4 has priority.
PCR Comments :



Length of sequence : 204  Consensus sequence is :

..

ATTATTGTGC TACTTACATT GCGCATCAAC AACAAAGCAC ACGACCAGAA 
TTAGCGAAAG TGGCACTAGC AGGTGCACAC GTTCTGTGTA CTTCGCCATG 
CACACAGACT TCGAATTACT TTGAACACTC AGTGATTTTG GAGGCACAAT 
ATGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGT

Go back to index

wmc(25a5) Plate reference : 25a5
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : No sequence 17.11.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc(25b5) Plate reference : 25b5
WMC locus number : WMC Length of sequence : 277
Last updated : Mar 22 DŽc 1998 at 9:41
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). Bases 152 to 235 show homology to 2h11 bases 85 to 168 (84 in longest run, maxScore = 59). No substantial microsatellite.
PCR Comments :



Length of sequence : 277  Consensus sequence is :

..

GCCTAAAGTC ATAGCAGGCT AGAATTGGCC CAACTGCAGA ATGGGCCGAG 
AAAAGGCCGA TGATGTTCCG GGCCGTTAAC GGGCTGGAAC TGACGATGGG 
CTGCTAACAG GCCGAAAGAA GCTCGGGCCG CAATTGGGCC CAAAAACGCA 
GCGGGTAAGG TTGGATGAAG GGTGGTAGGA ACAAATGCTC CGCCTAGAGC 
ATGTGTGTGT GAGATGGAGT CTTAGTGTGT GTGTGTGGGG CGTGTGTGTG 
TGTGTGAGAG AGAGAGGAGA GAGAGAT

Go back to index

wmc(25c5.1) Plate reference : 25c5.1
WMC locus number : WMC Length of sequence : 156
Last updated : Mar 29 DŽc 1998 at 16:09
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at both ends. Bases 68 to 104 (complement) show homology to 10e2 bases 53 to 89 (37 in longest run, maxScore = 85). (CA)4 1 to 8, (CA)9 13 to 30. No room for primers.
PCR Comments :



Length of sequence : 156  Consensus sequence is :

..

CACACACATA CGCACACACA CACACACACA AGTAAACTAG AAGACCGTTC 
TTTCATGTAT ACAAAACGTA TCGGCATCTG TCTATGTCTC ACTCATGCAT 
GATCATTTGC ACATTCACAC ACCTCTATGT CTGTATCACA TGCACACCGT 
CTCCAC

Go back to index

wmc(25c5.2) Plate reference : 25c5.2
WMC locus number : WMC Length of sequence : 276
Last updated : Mar 29 DŽc 1998 at 16:09
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 87 to 276 show homology to 10h2 bases 43 to 232 (190 in longest run, maxScore = 102). (CA)30 86 to 145. Duplicate, 10h2 has precedence.
PCR Comments :



Length of sequence : 276  Consensus sequence is :

..

CTCGTGGCTA AATCATACAC TACACACCAT CTCTCTGTAG TAGTACGTCG 
ATGTGTCGCT CTCTCTAACA CACACGCTAT TAAAACACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACATACAC 
ACACGCACGC ACGCACGCAC GCACACACGC ACACATAGGT TCATAGAATA 
AGAATATCAT GCGAGTGGGA AGCCAGGAAG TGAGATAAAT GGAGCATATA 
CAACAAGAGA AGTGGTGATG AATATT

Go back to index

wmc(25d5.1) Plate reference : 25d5.1
WMC locus number : WMC Length of sequence : 499
Last updated : Mar 30 mai 2000 at 9:54
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the first insert. Trimmed to insert at both ends. Bases 330 to 357 show homology to 20d3 bases 135 to 162 (28 in longest run, maxScore = 35). No substantial microsatellite. Bases 206 to 213 show homology to TW3g01.2 bases 162 to 169 (8 in longest run, maxScore = 21).
PCR Comments :



Length of sequence : 499  Consensus sequence is :

..

TGTTATGTGC AAAAACAACC TGACCTCTCG CATCCGCTCG ACACGTGGAA 
GCTGAAGTTG TTGATCCACA GAAGCCGAAG GGTACATACA TTGAATGGAA 
GGCCGAATAC ACTACTTGGA GGTCATTGGT GGAGGAACCA GCCTTGCAAT 
GCCGAAGAAA ATCTATGCAC CAAACACCTC ACCATTGAAG ACTGGTTCGG 
GGGCTACTGA GGGAGTCCTG GACTAAGGGG TCCTCGGGCG TCCAGCTTGT 
TAACTATGGG CCGGACTGAT GGGCTATGAA GATACGAAGA CTGAAAACCG 
AAGACTGCAC CCGTGTCCGG GTGGGACTCT CCTTGGCGTG GAAGGCAAGC 
TTGGCGACCA AATATGTAGA TTCCTTTCTT TATAACCGAC CTTGTGTAAC 
CCTAGATCAT CCCGGTGTCT ATATAAAACG GAGGACTTAG TCCGGAGGGG 
GAAAAATCCT CATTACCATA GCCAAACAAG CTAGACCTCT AGGGTTTAG

Go back to index

wmc(25d5.2) Plate reference : 25d5.2
WMC locus number : WMC Length of sequence : 347
Last updated : Mar 29 DŽc 1998 at 16:12
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Multple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 136 to 190 show homology to 2h11 bases 69 to 123 (55 in longest run, maxScore = 142). (GA)8 267 to 282. Duplicate, 2h11 has precedence.
PCR Comments :



Length of sequence : 347  Consensus sequence is :

..

GATCATATGG TCTGTATTAC AAGATGGCAG GCACACCATC ATCGCCAACT 
CGTTTTTTAT AAGGGTATAC AGATGATAAG TTTGCTGACT ACTAAAGTAA 
AGTGGAATCT GTCCATGTTA TGCAAGTAAA TAAAAGTGAT TGTTTATCAT 
ACGGGTAAGG TTGGATGAAG GGTGGTAGGA ACAAATGCTC TGCCTAGAGC 
ATGTGTGTGT GAGATGGAGT CTTAGTGTGT GTGTGGTGTG TGTGTGTGAG 
AGAGAGAAAG AGACAGGAGA GAGAGAGAGA GATGGAGTCT TAGTTTGTGT 
GTGTGTGAGA GAGAGAGATG GAGTCTAAGT GTGTGTGTGT GGGGGGG

Go back to index

wmc(25e5) Plate reference : 25e5
WMC locus number : WMC Length of sequence : 329
Last updated : Mar 29 DŽc 1998 at 16:14
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)17 236 to 269, (CA)6 295 to 306. No room for primers.
PCR Comments :



Length of sequence : 329  Consensus sequence is :

..

AATATTAAAT GAATGCATTA TGAAAATATA TCTAATGGTG AATATATTGA 
TATTGATTTG GTATTGTTTA GCTTCACATT TTTTATATGT TTGGTCAAAC 
CTAGAAAGTG TTAACTTTTT GACTCAGTTT ACACACCATG AATTTGGGAG 
GGAGGGAGTG ATGAACTTTA AATTCAGATG TCCAAGTGCT TTAAGAAAAC 
CAGAGAAATT ATCATATGCA CACACACACA CCCAACACAC ACACACACAC 
ACACACACAC ACACACACAC TGGAGCTTGA CAAAATACGG GCCCCACACA 
CACACACCCA ACACACACGC ACACGCGCG

Go back to index

wmc480(25f5) Plate reference : 25f5
WMC locus number : WMC480 Length of sequence : 252
Last updated : Mer 21 Juil 1999 at 16:16
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 111 to 148 (complement) show homology to 9b1 bases 283 to 320 (38 in longest run, maxScore = 73). (GT)5 139 to 148, (GT)6 151 to 162, (GT)12 229 to 252, Bases 114 to 148 show homology to 26e12.1 bases 41 to 75 (complement) (35 in longest run, maxScore = 76). Duplicate with 26e12.1, 25f5 has priority.
PCR Comments : 61C anneal, product.
Forward primer details :
cTgTcATTgTTggggTggg at 60 length 19 bases, Tm 64
Reverse primer details :
gAcAccAAAggAgTAgggcg at 204 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 164 bases.



Length of sequence : 252  Consensus sequence is :

..

AATATTCGAG AGAGAAACCA ATTGACAGAT TACGATCAGA TTGAGTTGTC 
ACCCTTCTGC TGTCATTGTT GGGGTGGGGG GAGGGAGAGA AAGACGTATC 
GAGAGTGTGA GCGGTAGGCA CAGAGGCACA ACTAGCGAGT GTGTGTGTAT 
GTGTGTGTGT GTTTGAAAGA GGAGTAGATA GATTACTACC AAGATCGAGG 
TGCCGCCCTA CTCCTTTGGT GTCGGGTAGT GTGTGTGTGT GTGTGTGTGT 
GT

Go back to index

wmc(25g5) Plate reference : 25g5
WMC locus number : WMC Length of sequence : 96
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 5' linker corrupted). No substantial microsatellite.
PCR Comments :



Length of sequence : 96  Consensus sequence is :

..

ACTACGCTTT GGAAATACCA TGGGCATAAG GGGAGGGGCA CAACTACGGC 
ACGCCCAGAA TGCGGCTTAA CCGCACTAGG GGCTCCCTAT TCTGTC

Go back to index

wmc(25h5) Plate reference : 25h5
WMC locus number : WMC Length of sequence : 153
Last updated : Mar 29 DŽc 1998 at 16:55
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 36 to 58 (complement) show homology to 2d11 bases 291 to 313 (23 in longest run, maxScore = 50). (CA)9 78 to 95, (CG)8 110 to 125, (ACCC)3 137 to 148. No room for primers.
PCR Comments :



Length of sequence : 153  Consensus sequence is :

..

GCCTCTTCCA TACACATCAC ATCTCTATGA TGAACCCATG CACACTTAAA 
TTACATCCAC CACAGAGGAC CCCGTGACAC ACACACACAC ACACAAACAC 
AAACACACAC GCGCGCGCGC GCGCGCACAT CCACTCACCC ACCCACCCAC 
ACA

Go back to index

wmc(25a6) Plate reference : 25a6
WMC locus number : WMC Length of sequence : 256
Last updated : Mar 29 DŽc 1998 at 16:56
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 207 to 256 show homology to 25e4.1 bases 173 to 222 (50 in longest run, maxScore = 88). (GT)8 85 to 100. Duplicate, 25e4.1 has precedence.
PCR Comments :



Length of sequence : 256  Consensus sequence is :

..

GCGAGAGTGG TCGCCGTATA ACCTTGGATG GGGATGAATT GTGTGCTCAG 
GGGAGTGTGT GTGTGTGTTG TGCTGCGTGC GTGCGTGTGT GTGTGTGTGT 
GCGTGCGTGC GTGTTTCTCT CTCTCTCTCC TTGTGTGTGT GTGTGTTAGA 
TATTGGGCGA AAACAAACCT AAGGAGAGAA ATGGGTATTC ACGAAGAGAT 
TGCGCAGGCC TTGAGGTGGG CAGGGGCCGA GTGAGGGTGT CAAAATGTGC 
GCGTTG

Go back to index

wmc481(25b6) Plate reference : 25b6
WMC locus number : WMC481 Length of sequence : 356
Last updated : Mer 30 DŽc 1998 at 10:43
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 1 to 54 (complement) show homology to 9f3.1 bases 252 to 305 (54 in longest run, maxScore = 66). (CT)13 90 to 115, (CT)16 197 to 228.
PCR Comments : 61C anneal, smear.
Forward primer details :
AcAcAAAAAccTTgTTggTccc at 158 length 22 bases, Tm 64
Reverse primer details :
gAcgAcATAgTggTgcATgAgA at 306 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 170 bases.



Length of sequence : 356  Consensus sequence is :

..

AATATTTGAA TGCAAGTTTA TATTGAAACA TGGTTTATAT CAGTCTTTGA 
TGCACAAAAC GCGACCTCCC CCTCTCCCGG ACTCTTGTTC TCTCTCTCTC 
TCTCTCTCTC TCTCTCACTC TCTCGCCGAC AATCTTCAAT TCTGTCACAT 
GCATCCGACA CAAAAACCTT GTTGGTCCCT CTCCCTTTCT CTCCATCTCT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTTT GTGTCTGTGT GGGGGGTCTT 
TCTAGTTCGC ATTCATATAT ACACCATCTA TTCCTTGGCT CTTTGCCTGT 
AACCCTCTCA TGCACCACTA TGTCGTCTCG GAGCTCTTCC CCCCCCCTAG 
TCCACG

Go back to index

wmc(25c6) Plate reference : 25c6
WMC locus number : WMC Length of sequence : 467
Last updated : Mer 30 DŽc 1998 at 10:47
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at both ends of insert. Bases 224 to 318 (complement) show homology to 11a4 bases 67 to 161 (95 in longest run, maxScore = 95). Bases 409 to 430 show homology to 20h12 bases 313 to 334 (22 in longest run, maxScore = 48). (CA)8 74 to 89, (CA)11 140 to 161, (CA)9 219 to 236. Duplicate, 11a4 has precedence.
PCR Comments :



Length of sequence : 467  Consensus sequence is :

..

GGCAGTAGTG CATACACGCA ACATCAGTGC GAGGGCAGGA GCACACACCC 
AGCAACACGT ACACATGCAG CAACACACAC ACACACACAC GCGCACACAC 
ACGCACCAAC ACACACACAC ACGCATGCAC ACGCGCGTGC ACACACACAC 
ACACACACAC ACGCAGCAGC ACACATGCAC GCGCACACAC ACGCAGCAAC 
ACACACACGC ATCACATACA CACACACACA CACACACGGC AGCACACACG 
CATGCAGCAC ATAGGCACGC AATAGCACAC ACACACGCGC GCATGCGCAC 
GTACACATGC AGCAGCACGC ATGCACACAC ACATGCAGCA GCAGCACACA 
CATGTGCGTG TGCACCCACA CACGCAACAA CACACACGCG CGCACACACA 
CATACCGTAT GAGGGGCAAA ATCGTCTTTT TAGGTGTCAT TTAGGCTCAA 
AATGGTCGGA AGTGTCG

Go back to index

wmc(25d6) Plate reference : 25d6
WMC locus number : WMC Length of sequence : 166
Last updated : Mer 30 DŽc 1998 at 10:50
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 47 to 108 show homology to 8h7 bases 698 to 759 (62 in longest run, maxScore = 50). Bases 67 to 98 show homology to 20c8 bases 32 to 63 (complement) (32 in longest run, maxScore = 56). (GA)13 128 to 153. Duplicate, 8h7 has precedence.
PCR Comments :



Length of sequence : 166  Consensus sequence is :

..

TTGGATGCGT GCGACAGAAC TAAAGATTGT CAGAGAGAGA GAGAGGGAGA 
GAGAGAGATG GAGGGAGAGG GAGAGGGACC AACAAGGTTT GTGTGTTGGA 
TGCGTGCGGC AGAACTGAAG ATTATCAGAG AGAGAGAGAG AGAGAGAGAG 
AGATGGAGGG AGAGGG

Go back to index

wmc(25e6) Plate reference : 25e6
WMC locus number : WMC Length of sequence : 156
Last updated : Mer 30 DŽc 1998 at 10:56
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 35 to 68 (complement) show homology to 6a11 bases 350 to 383 (34 in longest run, maxScore = 28). (CA)6 31 to 42, (CA)9 73 to 90, (CA)6 93 to 104, (TC)5 139 to 148, (AC)4 149 to 156. No room for primers.
PCR Comments :



Length of sequence : 156  Consensus sequence is :

..

TACACACACG CACACATGCA CGCACCACCC CACACACACA CACTAAGACT 
CCATCTCTCT CTCTCTCTTT CTCACACACA CACACACACA AGCACACACA 
CACACGCACC CCCCCCACAC ACACACGCTT AGACTCCATC TCTCTCTCAC 
ACACAC

Go back to index

wmc482(25f6) Plate reference : 25f6
WMC locus number : WMC482 Length of sequence : 463
Last updated : Mer 30 DŽc 1998 at 11:21
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 292 to 324 (complement) show homology to 2d8 bases 73 to 105 (33 in longest run, maxScore = 77). (CA)4 84 to 92, (CA)5 98 to 107, (CA)5 177 to 186, (GGC)5 282 to 296.
PCR Comments : 61C anneal, product.
Forward primer details :
ccTggccAcTAcTgggcATA at 36 length 20 bases, Tm 65
Reverse primer details :
cTgccAcTgTTTgcTgcTTg at 336 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 320 bases.



Length of sequence : 463  Consensus sequence is :

..

CACACGCACC TAGTAGTACT AAATCTAAGC AAGGGCCTGG CCACTACTGG 
GCATAGCCCA GCCCGCCACA CGCACGGCAC ACTCACACAC CACGCTGCAC 
ACACACACCA CGCTGCTCTC AGCACACACG GGCGCACACG GCCATGTCCA 
GCTGCACGGA CACACACTAT GCAGAACACA CACACACTAC ACTCATAGCT 
CGGCCATGGC CGGCGTAGCA GCGGCGGCAG CGAGCAGCGG CATCGACAGA 
CAGTAGCGGC AGCAACGGGA GCGACGCAGA AGGCGGCGGC GGCGGCAAAC 
AGCAGCGACG GCACAAGGGG CAGCTAGCAG CAGTACAAGC AGCAAACAGT 
GGCAGTAGAG CGGCAGTGTA GGCGTAGGCA GGCATGAACG GCCAGGGGCG 
CGGCGGTAGA AGGAGCAGTA GCAGCTAGCG CGGGCAGAGG AGCAATGGCA 
CAACAGCAAC AGC

Go back to index

wmc(25g6) Plate reference : 25g6
WMC locus number : WMC Length of sequence : 195
Last updated : Mer 30 DŽc 1998 at 11:32
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB3' linker corrupted). (CA)19 117 to 154, (CA)6 157 to 168. Too difficult to make primers.
PCR Comments :



Length of sequence : 195  Consensus sequence is :

..

GTACACTGCA ATACCTTTCC AATTAAGTAT TGCAAATCAT TATACCCGTT 
AGTGTCTATA CCTACCACGT CGGCACACAT CCTAAAGCAA TCCGAAGGTT 
TATATATGTA TATAGACACA CACACACACA CACACACACA CACACACACA 
CACACGCACA CACACACATA CATACATACA TCTGAGCTAT TCTGT

Go back to index

wmc(25h6) Plate reference : 25h6
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Jizeng, Beijing Date at Agrogene : 17.11.98 Public Domain : Yes
Comments : No sequence 17.11.98.
PCR Comments :
No sequence available for this clone
Go back to index

wmc483(25a7) Plate reference : 25a7
WMC locus number : WMC483 Length of sequence : 425
Last updated : Mer 30 DŽc 1998 at 14:14
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)28 290 to 345.
PCR Comments : 51C anneal, product (complex, possible dominant marker).
Forward primer details :
AAAATccTAggTgTgcTcTgg at 137 length 21 bases, Tm 60
Reverse primer details :
gTggAgTAgTgggTcAAAgTg at 380 length 21 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 264 bases.



Length of sequence : 425  Consensus sequence is :

..

CAGGTAACTG CTCTGGCTCC TCAAACAGAG CAGGTGTCTC CTCTACCGCG 
GGTGACGCCT CCTGTGCCCG AGCCTGTGGT CGTCGGCGAT GTCAACAAAG 
AAAACGTTAG TCATCTATCT CCTTTGATTT TTTTGTAAAA TCCTAGGTGT 
GCTCTGGACA GTCCTTTTGT CTATTTTTGG GGAAGGCCAG GGTTTCATTT 
TATCTTTGAT CGATGGACTC CATATCAGTA TTCTGTAGAT GTGTTAGGTA 
ACTAGCTAGG TTACATGTGT TGCGCCCTGT ATAGGCAATC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACACAAAGCT 
TGTGGCAACC ATGCCATGCA AACTCACGCC ACTTTGACCC ACTACTCCAC 
TACCTACACG CACGCCATGC ACAGC

Go back to index

wmc(25b7) Plate reference : 25b7
WMC locus number : WMC Length of sequence : 214
Last updated : Mer 30 DŽc 1998 at 14:15
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Forward sequence only. Trimmed to insert at both ends. (TTG)40 2 to 121, (TTG)20 125 to 184, (TTG)9 188 to 214. No room for primers.
PCR Comments :



Length of sequence : 214  Consensus sequence is :

..

GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT 
TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG 
TTGTTGTTGT TGTTGTTGTT GCTGTTGTTG TTGTTGTTGT TGTTGTTGTT 
GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGCTGTTG TTGTTGTTGT 
TGTTGTTGTT GTTG

Go back to index

wmc484(25c7) Plate reference : 25c7
WMC locus number : WMC484 Length of sequence : 375
Last updated : Lun 30 oct 2000 at 16:59
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 100 to 145 (complement) show homology to 8g4 bases 173 to 218 (46 in longest run, maxScore = 106). Homology to one side of microsatellite, reverse primer has several misamatches to 8g4. Forward primer outside region of homology. Bases 218 to 255 show homology to 20e7 bases 116 to 153 (complement) (38 in longest run, maxScore = 93). (GA)4 79 to 86, (GA)23 95 to 140. Bases 287 to 307 show homology to TW3g04.1 bases 192 to 212 (21 in longest run, maxScore = 32). Bases 94 to 141 show homology to TW2f01 bases 43 to 90 (48 in longest run, maxScore = 120). Bases 94 to 144 show homology to TW5c07 bases 72 to 122 (51 in longest run, maxScore = 78). Bases 220 to 253 show homology to TW7f06.1 bases 162 to 195 (34 in longest run, maxScore = 120).
PCR Comments : 61C anneal, smear (140-240).
Forward primer details :
AAATAggAcTAgccAccccc at 15 length 20 bases, Tm 61
Reverse primer details :
gTgTggTggATTgccTcTTg at 235 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 240 bases.



Length of sequence : 375  Consensus sequence is :

..

TGGCCTTTGC CTATAAATAG GACTAGCCAC CCCCAAGGGA GAGGGATGGG 
AAACAGAATC GGAGAGGGAT CGAAATCAGA GAGAGAGTCA AGAAGAGAGA 
GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA AGGGTGACTG 
AACTCCTCCT AGCAGTTCAT CCCCTCAGCC AAGAACAGAC CTTCGCGAGG 
CTGTTCTTCC TTGTATTGCT CATCATCATC AGCCCAAGAG GCAATCCACC 
ACACCACACA CTGGAGTAGG GTATTACGCC ACAACGGTGG CCCGAACCAG 
TATAAACCCT GTGTCTCTTG TGTTGTTCTT TCCATAGCTT AGATCTTAGC 
GAGGCGGAGG GGTGTAGGTA GGTAG

Go back to index

wmc485(25d7.1) Plate reference : 25d7.1
WMC locus number : WMC485 Length of sequence : 454
Last updated : Mer 30 DŽc 1998 at 14:46
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 277 to 338 (complement) show homology to 24e6 bases 230 to 291 (62 in longest run, maxScore = 105). (CA)13 341 to 366, (CA)7 381 to 394.
PCR Comments : tested 61C anneal, no product; 51C anneal, polymorphic on acrylamide (but complex).
Forward primer details :
cAgcgATggATAATTgTgTTgg at 219 length 22 bases, Tm 64
Reverse primer details :
cTTggATggggATgAATTgTgT at 406 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 209 bases.



Length of sequence : 454  Consensus sequence is :

..

AATATTCGCA AGTATTAGTA GTTGAGTTGT CAATTCAACC ACACCTGAAA 
GACTTAATAT CTGTTGCAAG GAGATCAGTA GCAATGTAGT ATGGCAATTT 
GATAGTAGCG GTAATAGTAG CAATAGTAAC ATTAACAGTT TTGTAGTGAT 
TGTAATAGTA GCAACGGCAA AGGTAAATTT GCAAAGATCA ATATATGAAA 
AGTGCGTAGG CATTGGATCA GCGATGGATA ATTGTGTTGG ATAATACACC 
CTCACCCGGC CCCTGCCTAC CTCAAAGCCC GCACAATCTC TTCGTGAATA 
CCCATTTCTC TCCTTAGGTT TGTTTTCTCT CAATATCTGA CACACACACA 
CACACACACA CACACAGACA TGCAGCACAA CACACACACA CACACTCCCC 
CGAGCACACA ATTCATCCCC ATCCAAGGTT ATACGGCGAC CACTCTCGCT 
TGTG

Go back to index

wmc486(25d7.2) Plate reference : 25d7.2
WMC locus number : WMC486 Length of sequence : 251
Last updated : Mer 30 DŽc 1998 at 15:00
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)28 68 to 123.
PCR Comments : 61C anneal, polymorphic on acrylamide (120-140 bases)
Forward primer details :
ccggTAgTgggATgcATTTT at 38 length 20 bases, Tm 64
Reverse primer details :
ATgcATgcTgAATccggTAA at 224 length 20 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 206 bases.



Length of sequence : 251  Consensus sequence is :

..

CCACATGATA GTATATGAGA AATGGGTGTA GCTACACCCG GTAGTGGGAT 
GCATTTTCCC TATATATGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTATTCAAA ACTTTCTTGA CATACATAGT 
CACCTACAGA CAAAAACGGC AATTATTTAG CTAGCACCTT GGTGTGAAAT 
TATACGTTGT TTTGCCGCTT GGATTACCGG ATTCAGCATG CATCAAATAT 
T

Go back to index

wmc(25e7) Plate reference : 25e7
WMC locus number : WMC Length of sequence : 194
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)31 132 to 193. No room for primers. Bases 114 to 194 show homology to 24A10 bases 81 to 161 (complement) (81 in longest run, maxScore = 119).
PCR Comments :



Length of sequence : 194  Consensus sequence is :

..

AGTGAAGAAT TCCAAAGGTG ATTGCTTTGG ATGGTATCGC GTTCATCGGT 
GCGTTTCCAA GCAAAAACTG CATTTTTTTC ACCTCCACGT GGGACACTAG 
GACGGCTCGT GTAATGTCGA CTACTACCAA TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTG

Go back to index

wmc487(25f7) Plate reference : 25f7
WMC locus number : WMC487 Length of sequence : 295
Last updated : Mer 30 DŽc 1998 at 15:06
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)20 120 to 159.
PCR Comments : tested 51C anneal, no product.
Forward primer details :
cAAATTTggccAccATTTTAcA at 53 length 22 bases, Tm 63
Reverse primer details :
cggTTcAATccTTggATTTAcA at 191 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 160 bases.



Length of sequence : 295  Consensus sequence is :

..

ACAAGCTCAG AGCACAACTT TTTCTCTAAG CATGCAACCA CAAATGAGTA 
CTCAAATTTG GCCACCATTT TACATTGGGT ACAAAAATGA GAATGATAAA 
TAAAAGTACA AAGCCTAGAC ACACACACAC ACACACACAC ACACACACAC 
ACACACACAC GGGCGCGCGT GTGAAAATAC AGCACATAGT TGTAAATCCA 
AGGATTGAAC CGGAGTGCGC CAGCTACACA AACTCCTTAC CAATGGGCCA 
CTACTCACTT CATTTCGCCG ATGTAAGTGA ATGCACATAA ATATT

Go back to index

wmc(25g7) Plate reference : 25g7
WMC locus number : WMC Length of sequence : 166
Last updated : Mer 30 DŽc 1998 at 15:06
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 86 to 160 show homology to 21a5 bases 106 to 180 (75 in longest run, maxScore = 71). (CT)11 138 to 159. Duplicate, 21a5 has precedence.
PCR Comments :



Length of sequence : 166  Consensus sequence is :

..

CAAACCTTGT TGGTTCCTCT CCCTTTCTCT CCATCTCTCT CTCTCTCTTT 
CTCTCTCTCT CTCCCCCCCA CAATCTTCAA TTCTGTCGCA CGCATCTAAC 
ACACAAACCT TGTTGGTCCC TCTCCCTTTC TCTCCATCTC TCTCTCTCTC 
TCTCTCTCTC CCCCAC

Go back to index

wmc(25h7) Plate reference : 25h7
WMC locus number : WMC Length of sequence : 142
Last updated : Mer 30 DŽc 1998 at 15:09
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 28 to 41 show homology to 20b4 bases 52 to 65 (14 in longest run, maxScore = 20). (CAA)25 7 to 81. No room for primers.
PCR Comments :



Length of sequence : 142  Consensus sequence is :

..

CAACAGCAAC AACAACAACA ACAACAACAA CAACAACAAC AACAACAACA 
ACAACAACAA CAACAACAAC AACAACAACA ACACCAGCGA CACCAGTAGC 
AGCAACAGCA ACAACAGCAA CAGCAACAAC AACACCAGCA AC

Go back to index

wmc(25a8) Plate reference : 25a8
WMC locus number : WMC Length of sequence : 200
Last updated : Mer 30 DŽc 1998 at 15:23
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 17 to 47 (complement) show homology to 13g1.2 bases 334 to 364 (31 in longest run, maxScore = 40). (CA)9 62 to 79, (CA)5 88 to 97, (CT)6 134 to 145, (CA)7 146 to 159, (TC)8 185 to 200. No room for primers.
PCR Comments :



Length of sequence : 200  Consensus sequence is :

..

CATCACCTCT ATGATCAAGC CATGCACACT TAAATTACAT CCGCCACGGG 
GGACCCCGCG ACACACACAC ACACACACAC GCACACGCAC ACACACACGC 
GCGCACCCCC CCCCACACAC ACTAAGACTT CATCTCTCTC TCTCTCACAC 
ACACACACAC GCCCCCACAC ACACTTAGAC TCCATCTCTC TCTCTCTCTC

Go back to index

wmc(25b8) Plate reference : 25b8
WMC locus number : WMC Length of sequence : 184
Last updated : Mer 30 DŽc 1998 at 15:29
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Forward sequence only. Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 84 to 146 (complement) show homology to 10d10 bases 258 to 320 (63 in longest run, maxScore = 63). (CA)9 25 to 42, (CA)14 45 to 72, (CA)5 77 to 86. Too difficult to make primers, due to relationship with 10d10.
PCR Comments :



Length of sequence : 184  Consensus sequence is :

..

CTTTGCATGT ATCAAGCATA TGACCACACA CACACACACA CAGACACACA 
CACACACACA CACACACACA CAGAGACACA CACACACGAA CACAACAACA 
ATCCAATAAG TATAGTGCAT CTATTTTTCC AAACCATGCA TGTATGCAAC 
AAATTCAAAA ATACTCATTC TATTCCTAAA TATT

Go back to index