Wheat microsatellite WMC 25/06/01

wmc(25c8) Plate reference : 25c8
WMC locus number : WMC Length of sequence : 153
Last updated : Mer 30 DŽc 1998 at 15:40
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 17 to 111 show homology to 10e8 bases 9 to 103 (95 in longest run, maxScore = 88). (CA)12 122 to 145. Duplicate, 10e8 has precedence.
PCR Comments :



Length of sequence : 153  Consensus sequence is :

..

GACCCAACGT ACTACGTGGA GAGAGGTAAG GTGACTAAGG CTGCATTATT 
TCTGCACCAC TGTGGATAGT CTTACCACCA AAGCCACATG ACACGATTAT 
GATTGAAATC CTAATGACTC CCACACACAC ACACACACAC ACACAAAAAG 
ACG

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wmc(25d8) Plate reference : 25d8
WMC locus number : WMC Length of sequence : 287
Last updated : Mer 30 DŽc 1998 at 15:41
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Forward sequence only. Trimmed to insert at 5' end, 3' end not located. Bases 49 to 94 show homology to 9d4 bases 243 to 288 (46 in longest run, maxScore = 105). (GT)11 40 to 61. Duplicate, 9d4 has precedence.
PCR Comments :



Length of sequence : 287  Consensus sequence is :

..

AGTGTGTGTG TGTGGGGGGG TGCGTGTGTG TGTGTGTTTG TGTGTGTGTG 
TGTGTGTGTG TGAGAGAGAG AGATGGAGTC TTAGTGTGTG TGTGTGGAGG 
GGGGTGCGTG CGTGTGTGTG TGTGTTGCGG GGTCCTCTGT GGCGGATGTA 
ATTTAAGTGT GCATGGCTTC ATCATAGAGA GGTGATGTGT ATGGTAGAGG 
CCACCAACGA TGGGGTGCGA GAGAGAAAAT GCCCGTACAG AGGGAAGAGA 
ACGTGTGCGC GCGTGAGAGG AGTCGACATC CAGAGAA

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wmc488(25e8) Plate reference : 25e8
WMC locus number : WMC488 Length of sequence : 265
Last updated : Mer 30 DŽc 1998 at 17:28
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 70 to 97, (GT)9 100 to 117.
PCR Comments : 61C anneal, polymorphic on acrylamide (120-140).
Forward primer details :
AAAgcAcAAccAgTTATgccAc at 39 length 22 bases, Tm 63
Reverse primer details :
gAAccATAgTcAcATATcAcgAgg at 151 length 24 bases, Tm 62
Expected size of amplified fragment (Chinese Spring=) 136 bases.



Length of sequence : 265  Consensus sequence is :

..

CACATGATTG TTGAGGTCTA TATATATGCT GCAACTTGAA AGCACAACCA 
GTTATGCCAC ATTTGACAAG TGTGTGTGTG TGTGTGTGTG TGTGTGTATG 
TGTGTGTGTG TGTGTGTATA GGTAGTGGTA TCTTTGTAAC TATACGTGAT 
CCTCGTGATA TGTGACTATG GTTCCCTAAT CTCTACACAG ATGGTTCCCT 
AATCTCTACA CAACTTCATG AATGGATAAA TTGCTCTAAG ATCTGCTTGG 
AGCTAGTCTA ATATT

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wmc489(25f8) Plate reference : 25f8
WMC locus number : WMC489 Length of sequence : 305
Last updated : Mer 21 Juil 1999 at 14:51
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 13 to 97 show homology to 7e6 bases 60 to 144 (85 in longest run, maxScore = 75). (CA)13 98 to 123. Bases 98 to 189 show homology to 26f11 bases 81 to 172 (92 in longest run, maxScore = 146). Duplicate with 26f11, 25f8 has priority.
PCR Comments : 51C anneal, polymorphic on acrylamide (various bands 176-270 bases, complex)
Forward primer details :
cgAAggATTTgTgATgTgAgTA at 37 length 22 bases, Tm 59
Reverse primer details :
ggAcAAcATcATAgAgAAggAA at 247 length 22 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 232 bases.



Length of sequence : 305  Consensus sequence is :

..

CGCAGGTTTG AACGGATGGA ATTCACACAC TTGGATCGAA GGATTTGTGA 
TGTGAGTATA TTTGTTCAGA AATACACATT TAAATTTGGT TAGTTTCCAC 
ACACACACAC ACACACACAC ACATGCATAG AACTTTAGCA TGTTCTTTTC 
TTCACGTAAT TGTGTTAATA ACAGGTAAAT CATAACTTTC TGTACTGATG 
GATGCATAAC ATAATACGAG TACTTGTTAC TTGAGTTTTG TTGACATTCC 
TTCTCTATGA TGTTGTCCTG TCTTAAGTTG AGTTAGGTCA GTGGGTTAGA 
ATATT

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wmc490(25g8) Plate reference : 25g8
WMC locus number : WMC490 Length of sequence : 270
Last updated : Lun 30 oct 2000 at 16:59
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 154 to 197 show homology to 1C2 bases 384 to 427 (44 in longest run, maxScore = 58). Forward primer is outsude this region of homology, but reverse primer matches 1c2. (GT)16 81 to 112, (CT)15 51 to 80. Bases 63 to 80 show homology to TW6a04.2 bases 84 to 101 (complement) (18 in longest run, maxScore = 30).
PCR Comments : 61C anneal, smear (120-200 bases)
Forward primer details :
AcTcTTTcgAccTcATggcg at 14 length 20 bases, Tm 64
Reverse primer details :
gAATAggTcgAATTgggcTcA at 162 length 21 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 169 bases.



Length of sequence : 270  Consensus sequence is :

..

CCAAGCCTCC CCCACTCTTT CGACCTCATG GCGGACCATG CTCGCTCAAT 
CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTCTCGATTG ACCTCGCTCT TTCTCGGACA AAAACGAGAT 
ACAACCATGT TTGAGCCCAA TTCGACCTAT TCACATTGTA TACTCTCACG 
CACATTGTTT ATCTAGGTCA CTCTCTCCAA CCCATCTCAC TCTTTCAATC 
GCACGACGAC CCACACTCGC

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wmc(25h8) Plate reference : 25h8
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : No sequence 2.12.98.
PCR Comments :
No sequence available for this clone
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wmc491(25a9) Plate reference : 25a9
WMC locus number : WMC491 Length of sequence : 277
Last updated : Mar 30 mai 2000 at 9:54
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 257 to 269 (complement) show homology to 12d2 bases 27 to 39 (13 in longest run, maxScore = 33). Bases 112 to 131 show homology to 25c11 bases 73 to 92 (20 in longest run, maxScore = 46). (GT)28 151 to 206, (GT)5 251 to 260. Bases 110 to 133 show homology to TW3f06.1 bases 167 to 190 (complement) (24 in longest run, maxScore = 22).
PCR Comments : 61C anneal, polymorphic on acrylamide (116-126 bases, 198-202 bases).
Forward primer details :
ggTAAAAcTTcgTgTcccTTgc at 56 length 22 bases, Tm 64
Reverse primer details :
TAgTTgcgAgTcggTAgTcTgc at 226 length 22 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 192 bases.



Length of sequence : 277  Consensus sequence is :

..

ATTATTAATC AAGTAGGACG TAGGGTTTTA CCTCCATCGA GAGGGCCCGA 
ACCTGGGTAA AACTTCGTGT CCCTTGCCTC ATGTTACCAT CCGGCCTAGA 
CGCACAGTTC GGGACCCCCT ACCCGAGATC CGCTGGTTTT AACACCGACA 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTCAAG GGTCTCCAGT TGCAAGCAGA CTACCGACTC GCAACTAGGG 
GTGTGTGTGT TTGTCGAGGT TCCCCGG

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wmc(25b9) Plate reference : 25b9
WMC locus number : WMC Length of sequence : 260
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 86 to 100 show homology to 1B2 bases 221 to 235 (15 in longest run, maxScore = 19). No substantial microsatellite.
PCR Comments :



Length of sequence : 260  Consensus sequence is :

..

GACCCAATCC AGAAACTAAG TTTTAGTCCA AACCAATCGG CTCAGATCCG 
CCTCCGTAGG GAGACGCGGT TGGGGAAGGG AAGAAGAGGG GAGATCTCAC 
ATACTTGCCG GAGTTGGCGG CGACCATGGA CCGGCAGTGA AGAGAGGGGT 
CGTCGGGAGA TGGAGGCGGC GGCGGCGGCG CTGGTCGAGA AGGGGAGAAA 
GAAAGATGTG GAGTGGTCGA GACGGAGAGA AAGAAAAATG TGGTACATGT 
GGTGGCTCGG

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wmc(25c9) Plate reference : 25c9
WMC locus number : WMC Length of sequence : 393
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 322 to 350 (complement) show homology to 24c3 bases 6 to 34 (29 in longest run, maxScore = 25). No substantial microsatellite.
PCR Comments :



Length of sequence : 393  Consensus sequence is :

..

CCGTATCGAT CCCTGCCTAA AACACCTTTC GCAACCCTAG CCGCCGCCAC 
CTCTAGCCGC CGCCACCACC ACCCTCCCTC ATTGTTGCTC CTCCCACTTG 
CAGCCGCTCC ACCCCAATAG CCGTCACGCT TCCCTTCTTC TCCCGCCGCG 
CCGCCGCTCC TCCCTCCCAT CTATAACCCT AGTCGCCGCC TCTTTCCTCC 
CACCCACCGC AAGCCTCCAC CTTTCCCGAT AATCATGAAG ACATGTTGAG 
ATTGAAGCCA AACCAAATAT GAAGACATTG CAAGGTAACG CCATGAGTGA 
AGTACTTCAA AATAGAACAT TTGGTGGTGG CGTTACCCAC CGTATAGGAA 
GTATTAGACC CAGACACGGC GCACAATTAT CGTGGTGCTC CGA

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wmc(25d9) Plate reference : 25d9
WMC locus number : WMC Length of sequence : 201
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end (NB 5' linker corrupted). Bases 76 to 104 (complement) show homology to 21f9 bases 98 to 126 (29 in longest run, maxScore = 70). (CA)8 32 to 47. Duplicate, 21f9 has precedence. Bases 142 to 185 show homology to 22c3 bases 14 to 57 (complement) (44 in longest run, maxScore = 45).
PCR Comments :



Length of sequence : 201  Consensus sequence is :

..

CACACACATA CACATACATA CACACACACT ACACACACAC ACACACATAC 
ACACACACAC TACACACACA GTACTACACA CACACATTTT TTTACTTATT 
TTCTATTTTC TATTGTTTAA GTTAATGTTA GATGAATTTC GTAGATAAGA 
ATGTTAGAAT GTTAATGTTA GATGAATTAG ATAGAAAAGT TGTTAAATAT 
T

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wmc492(25e9) Plate reference : 25e9
WMC locus number : WMC492 Length of sequence : 349
Last updated : Jeu 31 DŽc 1998 at 11:03
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 5 to 29 (complement) show homology to 13A1 bases 164 to 188 (25 in longest run, maxScore = 31). (CA)8 260 to 275, (CA)8 278 to 293, (CA)9 296 to 313. Primers share a motif, small inverted repeats near the microsatellite.
PCR Comments : 51C anneal, polymorphic on acrylamide (126-154 bases).
Forward primer details :
AggATcAgAATAgTgcTAccc at 192 length 21 bases, Tm 57
Reverse primer details :
ATcccgTgATcAgAATAgTgT at 317 length 21 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 146 bases.



Length of sequence : 349  Consensus sequence is :

..

CCTAGTTTTG TCACAATTAT CGTCATAGAG GTGTCATAAG TATGACATAA 
TTTTTTCGTT CAGCCCAAAA TGTCACGATG TGTCTTTTTT TGGTAGTGTG 
GGACTTTTTT CTGCAGCGGG TTGGGTAGTT CGGGAACAAC CCGGTCGCTC 
TATATAGGGC GTGACGTGAT TCAAATAGTT ATTCTGATCC CAGGATCAGA 
ATAGTGCTAC CCTATATATA GGGCTGACTT TGGATCCAAA TAGTTATTCT 
AATTCCATAC ACACACACAC ACACATACAC ACACACACAC ACATACACAC 
ACACACACAC ACATAAACAC TATTCTGATC ACGGGATCAG AATAATATT

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wmc(25f9) Plate reference : 25f9
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : No sequence 2.12.98.
PCR Comments :
No sequence available for this clone
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wmc493(25g9) Plate reference : 25g9
WMC locus number : WMC493 Length of sequence : 262
Last updated : Jeu 31 DŽc 1998 at 11:19
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 4 to 97 (complement) show homology to 3c4 bases 65 to 158 (94 in longest run, maxScore = 77). (TC)5 104 to 113, (CA)19 139 to 176, (CT)11 117 to 138.
PCR Comments : 61C anneal, smear (120-200 bases)
Forward primer details :
AcATAcAATgggATTccAgcAg at 57 length 22 bases, Tm 63
Reverse primer details :
AAcAAAcggTgTTcATgcAAgT at 221 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 186 bases.



Length of sequence : 262  Consensus sequence is :

..

ATAATGTCTC TCACACATAT GCCTAAGGTT AACTCGAGTT CCGGAACCAT 
ATGAATACAT ACAATGGGAT TCCAGCAGAG CACCTTTTGT TTTGAGATTT 
TGTTCTCTCT CTCTTTCTCT CTCTCTCTCT CTCTCTCTCA CACACACACA 
CACACACACA CACACACACA CACACATGCA CGCGCGCGCA CACATTGTTT 
CTCGCTTCAT TTTTTCTGGC ACTTGCATGA ACACCGTTTG TTTATCTTCG 
TGATGCTTTC AG

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wmc(25h9) Plate reference : 25h9
WMC locus number : WMC Length of sequence : 319
Last updated : Jeu 31 DŽc 1998 at 11:23
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to insert at both ends. Bases 188 to 212 (complement) show homology to 10h7.2 bases 19 to 43 (25 in longest run, maxScore = 33). (CA)4 2 to 9, (CA)4 12 to 19, (CA)6 40 to 51, (CA)7 78 to 91, (CA)5 95 to 104, (CA)8 117 to 132. No room for primers.
PCR Comments :



Length of sequence : 319  Consensus sequence is :

..

ACACACACAT ACACACACAC TACACACACA TACACACACC ACACACACAC 
ATACACGCAC ACACACATTC ACACATACAC ACACACACAC ACTACACACA 
CACAGGCATA CACATACACA CACACACACA CATTTTTTTA GTAAATTTTT 
ATTTTTAGTA AATGCTTAAT TGAACTAATT GAATTACTAT AACTAGTTTA 
TTTTTAGTAA ATTTTTAGTT GAACTAGTTG AACTAATAGA ACTAGTTTAT 
TTTTAGTAAA TGCTTTGTTG AACTAATTGA ATTAATAGAA CTAGTTTATT 
TTTAGCAAAT TAGTCCACG

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wmc494(25a10) Plate reference : 25a10
WMC locus number : WMC494 Length of sequence : 241
Last updated : Jeu 31 DŽc 1998 at 11:28
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)31 138 to 199.
PCR Comments : 51C anneal, polymorphic on acrylamide (160-220 bases).
Forward primer details :
ggATcgAgTcTcAAgTcTAcAA at 13 length 22 bases, Tm 58
Reverse primer details :
AgAAggAAcAAgcAAcATcATA at 209 length 22 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 218 bases.



Length of sequence : 241  Consensus sequence is :

..

GCCTTGGAGT ATGGATCGAG TCTCAAGTCT ACAACAACCT AGCCGCACCA 
TGTACTTGTT TGCCTATTTA AACACGCACG CATCCCTGCG GTATGCATAC 
GCTTCTAATC TTAGTTCTTT CACCTTGTTA CTTTCATGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTT 
GATTCTTCTA TGATGTTGCT TGTTCCTTCT TGTGTGTGTT G

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wmc495(25b10) Plate reference : 25b10
WMC locus number : WMC495 Length of sequence : 278
Last updated : Jeu 31 DŽc 1998 at 11:42
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 165 to 199 (complement) show homology to 25e4.1 bases 194 to 228 (35 in longest run, maxScore = 111). Forward primer is outside this region of homology, reverse primer has a mismatch to to 25e4.1. (GA)13 72 to 97, (GT)9 54 to 71.
PCR Comments : 51C anneal, smear.
Forward primer details :
AcgTTgTgTATAcATgcgAgAA at 14 length 22 bases, Tm 60
Reverse primer details :
AgcgAgAAcAAAccTAAggAgA at 250 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 258 bases.



Length of sequence : 278  Consensus sequence is :

..

GACAGATGCC CATACGTTGT GTATACATGC GAGAACGGTT TTCTAGTTTA 
CTGGTGTGTG TGTGTGTGTG TGAGAGAGAG AGAGAGAGAG AGAGAGAGGA 
GCATCCATAA CACAACAACC ATCTCTCTCG ATTCGTCCGT CCCTTGCACG 
ATCGCATGCA ACACATGCAT CAACGCGCAC ATTTTGACAC CCTCACTCGA 
CGCCTGCCCA CCTCAAGGCC CGCGCAATCT CTTCATGAAT ACCCATTTCT 
CTCCTTAGGT TTGTTCTCGC TCAATATT

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wmc(25c10) Plate reference : 25c10
WMC locus number : WMC Length of sequence : 87
Last updated : Jeu 31 DŽc 1998 at 11:51
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). Bases 34 to 63 show homology to 5g3 bases 204 to 233 (30 in longest run, maxScore = 60). No substantial microsatellite. Duplicate, 5g3 has precedence.
PCR Comments :



Length of sequence : 87  Consensus sequence is :

..

CACCGATTCT TTGGAGCGGC GCTGACCGTT TCGGGCGGGA AGCGCGCGCG 
GGCGATGGAG GGGATTTGGG TGGGCCAGGC TGGTCAG

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wmc(25d10) Plate reference : 25d10
WMC locus number : WMC Length of sequence : 132
Last updated : Jeu 31 DŽc 1998 at 11:54
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 65 to 106 show homology to 3e12 bases 186 to 227 (42 in longest run, maxScore = 69). Bases 1 to 132 show homology to 25d12 bases 1 to 132 (132 in longest run, maxScore = 87). (CA)15 1 to 30. Duplicate, 3e12 has precedence.
PCR Comments :



Length of sequence : 132  Consensus sequence is :

..

CACACACACA CACACACACA CACACACACA TACACCCCTA GTTGCGAGTC 
GATGGGCAAC TTGTAACTGG GGGTCCTCAA CAAACACGCA CCCCCTAGTT 
GTGAGTTGAT GCTCGACATG CAACAGGGAA CC

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wmc496(25e10) Plate reference : 25e10
WMC locus number : WMC496 Length of sequence : 247
Last updated : Lun 9 oct 2000 at 14:43
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 65 to 107 (complement) show homology to 11g1 bases 73 to 115 (43 in longest run, maxScore = 35). (GA)14 66 to 93. Bases 137 to 154 show homology to TW1f11 bases 131 to 148 (18 in longest run, maxScore = 39). Bases 65 to 93 show homology to TW5d05 bases 29 to 57 (29 in longest run, maxScore = 57).
PCR Comments : 51C anneal, smear.
Forward primer details :
gAcTAccgccAAAAgAcTTAcA at 5 length 22 bases, Tm 60
Reverse primer details :
ccAgATcAAATcTAgAcAccgT at 192 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 209 bases.



Length of sequence : 247  Consensus sequence is :

..

CGTGGACTAC CGCCAAAAGA CTTACATCAT ATGGATCTCC AAGAGACCAT 
TGTATTGATA ATAAAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGGAAGCC 
ATCTAGCCAC TAACTACGGA CCCGTAGGTC TACAAAGAAC TACTCACGCA 
TCATAAGAGA GGCACCAATG GAAGTGGTGA ACCCCTCCAT GACGGTGTCT 
AGATTTGATC TGGTGGTTCT GGACTTTGCG GCGGCTGGAT CAATATT

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wmc(25f10) Plate reference : 25f10
WMC locus number : WMC Length of sequence : 243
Last updated : Mer 21 Juil 1999 at 11:46
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 70 to 117 (complement) show homology to 6A1 bases 152 to 199 (48 in longest run, maxScore = 107). (GT)12 5 to 28, (GT)14 31 to 58. No room for primers. Bases 196 to 219 show homology to 26b10.1 bases 25 to 48 (complement) (24 in longest run, maxScore = 65). Duplicate with 26b10.1, 25f10 has priority.
PCR Comments :



Length of sequence : 243  Consensus sequence is :

..

ATATGTGTGT GTGTGTGTGT GTGTGTGTGC GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGA TTCGAAAGAT AGCAGTCGTT GGCTTCAGCC CCCACGCATA 
CAATGCGGGG GTGTTCGCGA AAAATTATGC ATGATCACTC TTTATCCAAC 
ATCTTGGTCC GGCAAGGAGG TGATCGCACG TCGAACTAGG CAACTGGACT 
ATATAGCTGT AACACTTTCT CTTAGCCGCG GGATTCTAAT ATT

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wmc(25g10) Plate reference : 25g10
WMC locus number : WMC Length of sequence : 304
Last updated : Jeu 31 DŽc 1998 at 12:02
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)17 227 to 260, (GA)19 261 to 298. No room for primers.
PCR Comments :



Length of sequence : 304  Consensus sequence is :

..

GAAGATTTGC ACACTCAAGG ACAAGTGTCT TTATTTGGTT CCATTGTCCA 
TGTTGTTGAA CTACCAGTAC TAACTTTGAG ACTTATATGA CCCTGTGTTT 
GGAACGCGAT AATTTTTGTG GGGGATATCA GTGAGTCCGT GTGGGTTGCT 
GGGATTCGGG AGGAGGTCCT TTTTATACGC ATATACGGCC GTGGGCGAGT 
CGTGCACGAT ACTTGGTAGG GGTTTTCACA CACACACACA CACACACACA 
CACACACACA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGC 
AGCC

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wmc(25h10) Plate reference : 25h10
WMC locus number : WMC Length of sequence : 241
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 241  Consensus sequence is :

..

CCACCGCTCA CCCTCGAGCA ACAACGCCGC ACGATACATC AATCCGCAGC 
CCGCACTGCT CAGGACAACC GCACGCAGAC CATGAACGCC GCCCCACATG 
CTGTCCAACC GTCCCGCCTG GGACACGCCG ACCGAGCCGA CAACCTTGCC 
GCCCACGCAG CTCAAGGTCC TCACCCAAGC CATGCTGACA AACAGCCCTA 
CCTCTTGGAG CCACCGCCGC ACCGCTGCCC GAGGCCGCCG C

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wmc497(25a11) Plate reference : 25a11
WMC locus number : WMC497 Length of sequence : 356
Last updated : Jeu 31 DŽc 1998 at 12:08
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)22 262 to 305.
PCR Comments : 61C anneal, polymorphic on acrylamide (210-220 bases).
Forward primer details :
cccgTggTTTTcTTTccTTcT at 109 length 21 bases, Tm 63
Reverse primer details :
AAcgAcAgggATgAAAAgcAA at 307 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 219 bases.



Length of sequence : 356  Consensus sequence is :

..

ATGAGAATTA TTGCATATTT TAGTTCTCTA GATTCATGCA AAAACTTTTG 
TCGCAGGCGC GTGCAGTGGT TTTTGTCTAG GCTATCATTC ACGCTTTGTC 
CCCCGCAGCC CGTGGTTTTC TTTCCTTCTG TATATACTGG GCCGTGCCCT 
GCCCTTCCTG TGTTTGTTGT GCGCCGTTTT CTTCCCAGCG CTGTAGCATG 
GGCCGAATGA TCGAAGAGTC AAGCCTTCCA TATAAACTTG CATGGTTGAC 
ATGCACATCA TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTTTTGC TTTTCATCCC TGTCGTTTGC CTTCTGTTAT TTTTGTGAAA 
CCTTTC

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wmc(25b11) Plate reference : 25b11
WMC locus number : WMC Length of sequence : 91
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). No substantial microsatellite.
PCR Comments :



Length of sequence : 91  Consensus sequence is :

..

GGAATGACTT ACCAGCGCTT TTGGACAGAT GACCTAACCT TCGGCACAAC 
AGATCTGGCA AGAGCTTGGA ACAGTAGAGA GCTCGATGCC T

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wmc(25c11) Plate reference : 25c11
WMC locus number : WMC Length of sequence : 604
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 73 to 92 show homology to 25a9 bases 112 to 131 (20 in longest run, maxScore = 46). No substantial microsatellite. Bases 41 to 53 show homology to 22g6 bases 51 to 63 (13 in longest run, maxScore = 22).
PCR Comments :



Length of sequence : 604  Consensus sequence is :

..

GAGAGGGCCC GAACCTGGGT AAACATCGTG TCCCCTGTCT CCTGTTACCA 
TCGACCTTAG ACGCACAGTT CAGGACCCCC TACCCGAGAT CCACCGGTTT 
TGACACCGAC ATTGGTGCTT TCATTGAGAG TTCCGTTGTG TCGTCGTCAA 
GAGGTTGGAT GGCCCCGTCG ATCATCTACA ACAATGTTGT CCAGGGAGAA 
ACCTTCCTCC CCGGACAGAT CTTCATATTC GGCGGCTTCG CACTGCGGGC 
CAACTCGCTT GGCCATCTAG AGCAGATCAA TAGCTACGCC CCTGGCCGTC 
AGGTCAGATT TGGAAGCTTG AACTACGTCG CGGATATCCG CGGAGACTTG 
ATCTTCGATG GATTCGAGAC CACGGCAGCC ACTCCCTGTT GCCACGATGA 
ACATGACTTA AATCTGTCAT CGGACCATGC CCAGGGGATA GCGCCTGTAG 
CTGCTCTGGC CCTAGATCTG GAGCAGATTG CGCCATCCGA GGACAGGAGG 
CTCAACCTTG CCACGGAAGC CGCAGACTCA GCAGCGTCGG AGCCGCACAC 
CGACCCAACC TCGAGTGGGA TCTGTGTCAC CGGAACCTCG GACCCGTTTC 
CGGC

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wmc(25d11) Plate reference : 25d11
WMC locus number : WMC Length of sequence : 6
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : No sequence 2.12.98.
PCR Comments :
No sequence available for this clone
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wmc498(25e11) Plate reference : 25e11
WMC locus number : WMC498 Length of sequence : 184
Last updated : Lun 4 Jan 1999 at 9:50
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 32 show homology to 2b1 bases 76 to 107 (32 in longest run, maxScore = 36). (GT)14 50 to 77, (GT)6 80 to 91, (GT)6 94 to 105, (GC)5 106 to 115.
PCR Comments : 61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (134 bases - gives null alleles).
Forward primer details :
cgATgAAgAgAgccATcAAAA at 27 length 21 bases, Tm 62
Reverse primer details :
TgAcATTccggTAggTcAgTT at 142 length 21 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 136 bases.



Length of sequence : 184  Consensus sequence is :

..

GAGGATCAAG TGGGTGGGTG CGAGATCGAT GAAGAGAGCC ATCAAAATTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGAG TGTGTGTGTG TGCGTGTGTG 
TGTGTGCGCG CGCGCGTGCA CGCAAGTCAA AGATATAGGG CAACTGACCT 
ACCGGAATGT CAGAGGGCAC ATGTCAAGTT TGTG

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wmc(25f11) Plate reference : 25f11
WMC locus number : WMC Length of sequence : 173
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 173  Consensus sequence is :

..

GGGGAGGAAA CGCATGATTC GGGGTTGCAG CTCTGGGCGA CGACGGCAGT 
GGTCGACAGT GGCTTGCGGT CCAGTGGAGG GTGGTGGATG GTTAGTTGGG 
GGCGGTGGCG GCGGTGGTGG ATGCTTGGGA GCGGCGGCGG CGGTGGATGC 
TCAGGGGTGG CGGCGGCTTG CGG

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wmc499(25g11) Plate reference : 25g11
WMC locus number : WMC499 Length of sequence : 302
Last updated : Lun 4 Jan 1999 at 10:00
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 5' linker corrupted). Bases 253 to 270 (complement) show homology to 2f12 bases 141 to 158 (18 in longest run, maxScore = 19). (GT)29 176 to 233.
PCR Comments : 61C anneal, polymorphic on acrylamide ((150-190 bases)
Forward primer details :
ggAAcTcTgTggTccAccTgA at 98 length 21 bases, Tm 64
Reverse primer details :
cggTgTgAccTAgcAcgAAcT at 272 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 195 bases.



Length of sequence : 302  Consensus sequence is :

..

GATGCCTTTG GTAGGCATGA GCCACCCATG ACTTAGCCCT GAGAGGGAGT 
GAGTTGGAGT GGCCGGGAGA GTGTCATGGC AAGGGAAGGG TTTCATCGGA 
ACTCTGTGGT CCACCTGAAT GGGGGTGCGA GGCCCTTGAG TTCTGTAGTG 
TGGGTAAAGT GCGCTAACCT CTGCAGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTTTAATCT ATCGATAGCC 
GCGTCCTCGG TCATGGGCAT AAGTTCGTGC TAGGTCACAC CGTTCGATCA 
AC

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wmc(25h11) Plate reference : 25h11
WMC locus number : WMC Length of sequence : 115
Last updated : Lun 4 Jan 1999 at 10:00
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (TTG)22 8 to 73, (TTG)13 77 to 115. No room for primers.
PCR Comments :



Length of sequence : 115  Consensus sequence is :

..

GTTGCTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT 
TGTTGTTGTT GTTGTTGTTG TTGTAGTTGT TGTTGTTGTT GTTGTTGTTG 
TTGTTGTTGT TGTTG

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wmc(25a12) Plate reference : 25a12
WMC locus number : WMC Length of sequence : 252
Last updated : Lun 4 Jan 1999 at 10:02
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 252 show homology to 22e10 bases 1 to 252 (252 in longest run, maxScore = 147). (CT)9 63 to 80, (CT)13 152 to 177. Duplicate, 22e10 has precedence.
PCR Comments :



Length of sequence : 252  Consensus sequence is :

..

CACACAAACC TTGTTGGTCC CTCTCCCTTT CTCTCCATCT CTCTCTCTCT 
CTGTCTCTCT GTCTCTCTCT CTCTCTCTCT CCCACAATCT TCAATTCTAT 
CGCACGCATC TAACACACAA ACCTTGTTGG TCCCTCTTCC CTTCTCTCCA 
TCTCTCTCTC TCTCTCTCTC TCTCTCTCAC CCACAATCTT CAATTCTGTC 
GCACGCATCT AACACACAAA CCTTGTTGAT CCCTCTCCTT TCTCTCCATC 
TC

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wmc500(25b12) Plate reference : 25b12
WMC locus number : WMC500 Length of sequence : 247
Last updated : Lun 4 Jan 1999 at 10:07
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)13 38 to 63, (CA)10 66 to 85.
PCR Comments : 51C anneal, polymorphic on acrylamide (polymorphism is superimposed on smear, 150-190 bases, complex).
Forward primer details :
ATAgcATgTTggAAcAgAgcAc at 15 length 22 bases, Tm 61
Reverse primer details :
cTTAgATgcAAcTcTATgcggT at 178 length 22 bases, Tm 60
Expected size of amplified fragment (Chinese Spring=) 185 bases.



Length of sequence : 247  Consensus sequence is :

..

GCAAAGTTGC TTCTATAGCA TGTTGGAACA GAGCACTCAC ACACACACAC 
ACACACACAC ACAGACACAC ACACACACAC ACACATGTTC AAGTAGAGGA 
ATTGGCACCG TCATACCCAC CCTGAGTGCG GATACTCCTC CTAAGTCCTA 
ACACATGGTT CAAGTAGAGG AATTGGCACC GCATAGAGTT GCATCTAAGT 
CCTAGTAGGT GTCCTCCACA CCGAGTTGCT GAATTCAGCC CGGGGAG

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wmc(25c12) Plate reference : 25c12
WMC locus number : WMC Length of sequence : 407
Last updated : Lun 4 Jan 1999 at 10:11
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 264 to 348 (complement) show homology to 2c1.2 bases 134 to 218 (85 in longest run, maxScore = 137). (GA)8 2 to 17, (GT)12 46 to 69, (GT)9 158 to 175, (GT)8 178 to 193. No room for primers.
PCR Comments :



Length of sequence : 407  Consensus sequence is :

..

TGAGAGAGAG AGAGAGATGG AGTCTTAGTG TGTGGGGGTG GGTCGGTGTG 
TGTGTGTGTG TGTGTGTGTG CGTGCGTGTG TGTGTGTGTA TGTGTGTGTG 
TGAGAGAGAG AAAGAGAGAG AGAGAGATGG AGTCTTAGTG TGTGTGGGGG 
GGTGCATGTG TGTGTGTGTG TGTGTTTGTG TGTGTGTGTG TGTCGCGGGG 
TCCTCGATGG CAGATGTAAT TTAAGTGTGC ATGGCTTCAT CAGGGAGAGG 
GGATGTGTAT GGTAGAGGCC ACCAACGATG GGGTGCGAGA GAGAAAATGC 
CCGTAGAGAG GGAAGAGAAG GTGTGTGCGC GTGAGAGGAG TCGACATCGA 
GAGAACGTGT TTGTTCAAGA GACACCGAGG AAGACTACCA TATATCGATG 
GTGCATG

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wmc(25d12) Plate reference : 25d12
WMC locus number : WMC Length of sequence : 132
Last updated : Lun 4 Jan 1999 at 10:13
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 132 show homology to 25d10 bases 1 to 132 (132 in longest run, maxScore = 87). (CA)15 1 to 30. Duplicate, 25d10 has precedence.
PCR Comments :



Length of sequence : 132  Consensus sequence is :

..

CACACACACA CACACACACA CACACACACA TACACCCCTA GTTGCGAGTC 
GATGGGCAAC TTGTAACTGG GGGTCCTCAA CAAACACGCA CCCCCTAGTT 
GTGAGTTGAT GCTCGACATG CAACAGGGAA CC

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wmc(25e12) Plate reference : 25e12
WMC locus number : WMC Length of sequence : 382
Last updated : Lun 4 Jan 1999 at 10:14
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to insert at both ends. Bases 336 to 355 (complement) show homology to 5c12 bases 134 to 153 (20 in longest run, maxScore = 29). (TTG)26 116 to 193, (TTG)10 353 to 382. No room for primers.
PCR Comments :



Length of sequence : 382  Consensus sequence is :

..

TCCGAAGAGC ACACGAGGTG CCCACGAGGC AGGGGGCGCG CCTAGAGGGG 
TAGGGCGCGC CCTCCACCCT CGTGGAAGCC TCGTGTCCTT TTTGGACTGC 
TTCTTATTTT CCTATTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT 
GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGCTGTTGT 
TGTTGTTGCT GTTGTTGTTG TTGCTGTTGT TGCTGTTGTT GTTGTTGCTA 
TTGTTGTTGT TGCTGTTGTT GTTGTTGTTG CTGTTGTTGC TGTTGTTGGT 
GGTGGTGGTG GTGCTGCTGT TGTTGTTGGT GGTGTTGTTG CTGCTGCTGC 
TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TG

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wmc(25f12) Plate reference : 25f12
WMC locus number : WMC Length of sequence : 220
Last updated : Lun 4 Jan 1999 at 10:17
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 96 to 203 (complement) show homology to 3e9.1 bases 1027 to 1134 (108 in longest run, maxScore = 44). (GT)27 158 to 211. No room for primers.
PCR Comments :



Length of sequence : 220  Consensus sequence is :

..

GATGTTTCCG AGGAGGTCCA CTCACATAAC TCACGGTGAA GCCTCTCGTT 
CGTCCAATGA AGTTGCACAT ATGCAACAAG TTCATTTTCA GGGGACGAAC 
TCCCTCGACC GCGCTGAAGA GAAGAACTAT TCGACTCCTT AGCATGAAAC 
CAACCAAGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TTGTGTGTTG

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wmc(25g12) Plate reference : 25g12
WMC locus number : WMC Length of sequence : 173
Last updated : Lun 21 DŽc 1998 at 16:26
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 173  Consensus sequence is :

..

TGGGATCCAG ATCGGGCACC CTAGCCGCCG ACGTGATCTC TTCATTGCTG 
CCGCCAGCAC GACCACCCTG GCCGCCACCG GCCTGCGCCA CCGCCCTTGA 
ATCGGTCGGA GTGGGTTGCT GCCACCCAGA TCCGATCGCC GGCCGAGGAA 
GATTGCCGCC GCCACCGCTG CGG

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wmc(25h12) Plate reference : 25h12
WMC locus number : WMC Length of sequence : 224
Last updated : Lun 4 Jan 1999 at 10:17
Owner : Serasem Date at Agrogene : 2.12.98 Public Domain : Yes
Comments : Reverse sequence only. Trimmed to insert at both ends. (CA)8 181 to 196, (CA)13 199 to 224. No room for primers.
PCR Comments :



Length of sequence : 224  Consensus sequence is :

..

CCTTTACTGT CAGATCCGTT CTCACGACAG GAAGGAAGGG GGAGGCGCCC 
TAAGGCAGCC AGCCCCCCTT TCCTCCCCCC CCATGTTCAA ATATGGAGGG 
GGGCTAGGCA TGCCTCTAGG GTAGCCGCGG CCACTTGGGG TGCCCTAGGG 
TTGCCTCCTC TCCCCCTCCA CACACACAAA CACACACACA CACACAGACA 
CACACACACA CACACACACA CACA

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wmc(20A7) Plate reference : 20A7
WMC locus number : WMC Length of sequence : 476
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : 5 ' end not located, trimmed to insert at 3' end (NB 3' linker corrupted) . Bases 133 to 239 (complement) show homology to 6h3 bases 159 to 265 (107 in longest run, maxScore = 129). (GT)8 291 to 306, (GT)10 309 to 328, (GT)13 391 to 416. Too difficult to make primers. Bases 189 to 238 show homology to 22h6 bases 120 to 169 (50 in longest run, maxScore = 121).
PCR Comments :



Length of sequence : 476  Consensus sequence is :

..

GATGTGGAGA GAGGAGCGGG TATCTTTTGT AAAATTATCA CAGTTTGCTT 
TCTATCCGTC AGATATAGAT CAGACAGTCT ATATTACAAG ATGGCAGGCA 
CACCATCATC GCCAACTTGG TTTTTTATAA GGGTACAGAT GATAAGTTTG 
CTAACTACTA AAGTAAAGTG GAATTTGTCC ATGTTATACA AGTAAATTAA 
GGTGATTGTT TATCATACGG GTAAGGTTGG ATGAAGGGTG GTAGGAAGAA 
ATGCTTCGCC TANAGCATGT GTGTGTGAGA TGGAGTCTTA GTGTGTGTGT 
GTGTGTCTGT GTGTGTGTGT GTGTGTGTTT GTGTGTGTGT GAGATGGAGT 
CTTAGTGTGT GTGTGTGTGC GTGTGTGTGA TGGAGTCTTA GTGTGTGTGT 
GTGTGTGTGT GTGTGTTTGT GTGTGGGTGG ATGGGTGCGT GTGTGCGTGC 
GTGTGTGTGT GTTTGTGTGT GTAGCC

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wmc501(20b7) Plate reference : 20b7
WMC locus number : WMC501 Length of sequence : 328
Last updated : Lun 4 Jan 1999 at 14:27
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. Bases 241 to 295 show homology to 4f3 bases 112 to 166 (55 in longest run, maxScore = 61), forward primer is not within this region of homology, reverse primer matches 4f3. (GT)30 244 to 303.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
cTcggcTTcAcTATcgTcgTcT at 105 length 22 bases, Tm 65
Reverse primer details :
TAcTTgTcAcATcggcccTcTT at 306 length 22 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 223 bases.



Length of sequence : 328  Consensus sequence is :

..

CGTGGACCAT ACCGGAGGAA CAAATTCTTG TGGCCCCTTT GACACTGGCC 
ACGAAAACCT TGAAGAGGCC ATTGGGCATC GGCCGGTTGC GGAACGAGCG 
TTCCCTCGGC TTCACTATCG TCGTCTTTAC CGCGTCCACC TTCTGGCTGA 
TGATGGTGTA CGGTATGGTG CACGGGGTTT CGTTGGCCTG CAAAGACATG 
TCTACGATGT CAGACATCCG GAAGGAAACG GAAACGGAAG ATTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTTGAAGAG GGCCGATGTG ACAAGTAG

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wmc(20c7) Plate reference : 20c7
WMC locus number : WMC Length of sequence : 428
Last updated : Lun 4 Jan 1999 at 14:28
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 84 to 132 (complement) show homology to 5c12 bases 173 to 221 (49 in longest run, maxScore = 43). (TTG)42 94 to 219, (TTG)15 247 to 291, (TTG)15 295 to 339. No room for primers.
PCR Comments :



Length of sequence : 428  Consensus sequence is :

..

TTGTTGTTCT TGTTGTTGTT GCTGTTGCTG TTGCTGTTGC TGTTAGTGTT 
GGTGTTGTTG CTGTTGCTGT TGCTGTTACT GTTGCTGTTG CTGTTGTTGT 
TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG 
TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT 
GTTGTTGTTG TTGTTGTTGC TGCTGCTGCT GCTGTTGTTG TTGCTGTTGT 
TGTTGTTGTT GTTGTTGTTG TTGTTGTTGT TGTTGTTGTT GCTGTTGTTG 
TTGTTGTTGT TGTTGTTGTT GTTGTTGTTG TTGTTGTTGG TGGTGTTGTT 
GTTGTTGGTG TTGTTGTTGT TGTTGTAGTA GACCACGCGC CTCGAGGGAT 
CATCAAGAGT CGACCTGCAA GCAAGCAA

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wmc(20d7) Plate reference : 20d7
WMC locus number : WMC Length of sequence : 167
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end, 3' end not located. Bases 62 to 97 (complement) show homology to 11a10 bases 185 to 220 (36 in longest run, maxScore = 57). No substantial microsatellite.
PCR Comments :



Length of sequence : 167  Consensus sequence is :

..

AATATTAGGG TTAGTTCATC TTATCCGAAC CAATATGAGC CTTTGCATAA 
TCTTTTCTGT ATTTGCTTTG TTGAATTTGC GGTTGCATCG TCGTGTCAAG 
TTGCTGGTCC TAGCGTCTAG TACTTTAGAG TTTCGACTTC TGTTCACAAG 
TTGTCACGCC GCCGCCG

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wmc502(20e7) Plate reference : 20e7
WMC locus number : WMC502 Length of sequence : 434
Last updated : Lun 9 oct 2000 at 14:43
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 116 to 153 (complement) show homology to 25c7 bases 218 to 255 (38 in longest run, maxScore = 93). Bases 263 to 357 show homology to 24a8 bases 23 to 117 (complement) (95 in longest run, maxScore = 48). (GT)14 262 to 289, (CT)14 232 to 259. Bases 222 to 259 show homology to TW2g04.2 bases 81 to 118 (complement) (38 in longest run, maxScore = 28).
PCR Comments : 51C anneal, product.
Forward primer details :
cgTTgTgggTgTTATAcccTAc at 83 length 22 bases, Tm 60
Reverse primer details :
AAgAAgggAgAATcTAgAAgcc at 327 length 22 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 266 bases.



Length of sequence : 434  Consensus sequence is :

..

CCTGCACCCT CCGCCTCGCT AAGATCTAAG CTATGGAAAG AACAGCACAA 
GAGACACAGG GTTTATACTG GGTTCGGGCC ACCGTTGTGG GTGTTATACC 
CTACTCCAAG TGTGGGGTGT GGTGGATTGC CTCTTGGGCT GATGATGATG 
AGCCATACAA GGAAGAATAG CCTCGCGGGG GTCTGTTCTT GGCTGGGGGG 
ATGAACTGCT AGGAGGAGTT CAGTCGCCCT TCTCTCTCTC TCTCTCTCTC 
TCTCTCTCTA TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG ATGCTACTCG 
ATCCGATCCT AGCTAGTTTC TCTCTTGGCT TCTAGATTCT CCCTTCTTGA 
TTGCCCCCTT CTTGATTGCC CCTCTACCCT GGGGGTGGCT AGTCCTATTT 
ATAGGCAAAG TCCCTGGGCC TCTTCCCAAA TATT

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wmc(20f7) Plate reference : 20f7
WMC locus number : WMC Length of sequence : 6
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : No sequence 8.12.98.
PCR Comments :
No sequence available for this clone
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wmc(20g7) Plate reference : 20g7
WMC locus number : WMC Length of sequence : 6
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : No sequence 8.12.98.
PCR Comments : 61C anneal, product.
No sequence available for this clone
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wmc(20h7) Plate reference : 20h7
WMC locus number : WMC Length of sequence : 6
Last updated : Mar 22 DŽc 1998 at 9:42
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : No sequence 8.12.98.
PCR Comments :
No sequence available for this clone
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wmc(20a8) Plate reference : 20a8
WMC locus number : WMC Length of sequence : 278
Last updated : Lun 4 Jan 1999 at 15:01
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 88 to 105 show homology to 8h1 bases 161 to 178 (18 in longest run, maxScore = 47). (GT)15 34 to 63, (GT)7 227 to 240. Too difficult to make primers. Microsatellite is embedded in minisatellite-like repeat region.
PCR Comments :



Length of sequence : 278  Consensus sequence is :

..

CCAGTTGCAA GCCGACCATC GATTTGCAAC TAGGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTTTGTCGA GGGTCCCTAG TTGCAAGCCG ACCATCAACT 
CGCAATTAGG GGGTGTTTGT TGATGGGCCC GAGTTGCAAG TCGACCATCA 
AACTCGCAAC TAGGGTGCGT GTGTGTTTTG TCGTGACCCC CAGTTGCAAG 
CCGACCATCG ACTCGCAACT AAGGATGTGT GTGTGTGTGT TTGTCGAGGG 
TCCCCAGTTG CAAGACGACC ATCGATTC

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wmc503(20b8) Plate reference : 20b8
WMC locus number : WMC503 Length of sequence : 503
Last updated : Lun 4 Jan 1999 at 15:06
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)11 112 to 133, (GT)18 192 to 227.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
gcAATAgTTcccgcAAgAAAAg at 55 length 22 bases, Tm 64
Reverse primer details :
ATcAAcTAccTccAgATcccgT at 270 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 237 bases.



Length of sequence : 503  Consensus sequence is :

..

CACATACCTC TGAAATCTTC CATATTTTCT TGGTCCTAGT CTCACATTTC 
ACTTGCAATA GTTCCCGCAA GAAAAGGTCA CTTGAAATAA TAGTTGAAAA 
AACAAATTGA AGTGTGTGTG TGTGTGTGTG TGTGGTCGGC GCACCATAGA 
TGTGAGGTAG TTGATTACAC ATCAACATCT TGTCTGAACA TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGGA ATAAGAATGA AATTCTGTGG 
TTGGCACACC ATAGATGTGA CGGGATCTGG AGGTAGTTGA TTCCATATCA 
ACATCTTGTC TGAACAAAGA CTTGGAATTC TAAACTAGGC TAGTCATGTG 
GTTCTTGCAG CACCAACTCA TAGCAAGGTT CCTTGTGGAG CAACTTCCAA 
AAGGACCTCC ACATATCAAT CCTANGTGTG AAGCTTATTT TGTCACTTTG 
TTAGTCAAAG TCGCGGCCTA TATATGCAGC CTAAAAGTTG AGGCATCTCT 
TTC

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wmc504(20c8) Plate reference : 20c8
WMC locus number : WMC504 Length of sequence : 161
Last updated : Lun 30 oct 2000 at 16:59
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : Trimmed to insert at 5' end, 3' end not located. Bases 32 to 63 (complement) show homology to 25d6 bases 67 to 98 (32 in longest run, maxScore = 56), reverse primer has 2 mismatches to 25d6, forward primer has no matches. (CT)7 67 to 80, (CT)7 85 to 98. Bases 36 to 80 show homology to TW6c12 bases 61 to 105 (complement) (45 in longest run, maxScore = 68). TW6c12 is classified as a duplicate of 20c12. 20c12 has priority.
PCR Comments : tested 61C anneal, no product. t 51C anneal, product(100-120130-226 bases).
Forward primer details :
gTTcTgTcgcAcgcATTcAA at 15 length 20 bases, Tm 65
Reverse primer details :
cATgccTgcAggTcgAcTcT at 141 length 20 bases, Tm 66
Expected size of amplified fragment (Chinese Spring=) 146 bases.



Length of sequence : 161  Consensus sequence is :

..

TCTGACAATC TTCAGTTCTG TCGCACGCAT TCAACACACA AACCTTGTTG 
GTCCCTCTCC CTCCATCTCT CTCTCTCTCT TTGTCTCTCT CTCTCTCTGA 
CAATCTTCAG TTCTGTCGCA CGCGCCTCGA GGGATCCTCT AGAGTCGACC 
TGCAGGCATG C

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wmc(20d8) Plate reference : 20d8
WMC locus number : WMC Length of sequence : 469
Last updated : Lun 5 juil 1999 at 17:23
Owner : Byrne, Colorado State Date at Agrogene : 8.12.98 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 124 to 230 (complement) show homology to 5d6 bases 19 to 125 (107 in longest run, maxScore = 129). (CA)4 34 to 41, (GA)744 to 57. Duplicate, 5d6 has precedence. Bases 433 to 459 show homology to 26c12 bases 304 to 330 (27 in longest run, maxScore = 105).
PCR Comments :



Length of sequence : 469  Consensus sequence is :

..

TGGACGAGAT AGTAGGAGAC AAACCTAACG AGACACACAC ATAGAGAGAG 
AGAGAGAAAG AGAGAGGAAG AATTAGGTCT GTGAGAAAGA TGGAGACATG 
TAACATACGT GATTCTGTAT ACGTGGAAGG AGAAGAGACA ACATGTTTGA 
TGAGAGAGCT GGAAAAACAT GGACGAGAGG GGAAGGATCT CATGGGTTGA 
GGGATTGACA ATTTTGTGCG GGGGAGAGAG GGATTGGTAA TTTTGTGCAA 
GAGAGAGAAG GTGGGGAGAG GAGTTAGTCG GTCGTCGTCA CATCACCCTG 
CTTCCAAATG ACCCCGCATT CTCTAACGTG GTGTGGTCGC CGGCTTCGAT 
TTGCTCGATT CCCTCCTTCA AGATTGAAGT GTTGTGCATG TTGGGGTGCA 
AAGAAGCACA AGAGAAAATG GTCGCCATAT AACCTTGGAT GGGGATGAAT 
TGTGTGCTCA AGGGAATGT

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