Wheat microsatellite WMC 25/06/01

wmc(26h2) Plate reference : 26h2
WMC locus number : WMC Length of sequence : 247
Last updated : Ven 12 FŽv 1999 at 15:27
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 247 show homology to 26f2 bases 1 to 247 (247 in longest run, maxScore = 186). (GT)12 107 to 130. Duplicate, 26f2 has precedence.
PCR Comments :



Length of sequence : 247  Consensus sequence is :

..

CGTGGACTAA TTCGTCACCT CTTCGCTTCT TGTACATACT CCATTTGTAT 
CTCACTTCCT CTGGCTTCGC ACTCGCATGA TTTTCCTATT CTATGAACCT 
GTGTGCGTGT GTGTGTGTGT GTGTGTGTGT TTAACAGCAT GTGTGTGTTA 
GAGAGAGCGA CAGATCGACC TACTCCTATA GAGTTATGGT GTGTAGTGTA 
TGATTTTAGC CGCGAAAGTC AGTGCATCCT CTCGTTTCCG GGCGTCC

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wmc(26a3) Plate reference : 26a3
WMC locus number : WMC Length of sequence : 226
Last updated : Ven 12 FŽv 1999 at 15:28
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 62 to 92 (complement) show homology to 21a12 bases 119 to 149 (31 in longest run, maxScore = 25). (GT)5 6 to 15, (GA)10 16 to 35, (GA)9 169 to 186, (GT)13 129 to 154, (GT)7 209 to 222. No room for primers.
PCR Comments :



Length of sequence : 226  Consensus sequence is :

..

GTTGGGTGTG TGTGTGAGAG AGAGAGAGAG AGAGATGGAG TCTTAATTTG 
TGTGTGTGAG AGAGAGAGAT GGAGTCTTAG TGTGTGGGGG GGATGTGCAT 
GCGTGTGTGC GTGCATGTGT GTGTGTTTGT GTGTGTGTGT GTGTGTGTGT 
GTGTATGTGT GTGTGTGTGA GAGAGAGAGA GAGAGACAGA GAGAGAGATG 
GAGTCTTAGT GTGTGTGTGT GTGGGG

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wmc(26b3) Plate reference : 26b3
WMC locus number : WMC Length of sequence : 191
Last updated : Ven 12 FŽv 1999 at 15:31
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 139 to 170 show homology to 7h1 bases 142 to 173 (32 in longest run, maxScore = 48). (GT)8 67 to 82, (GT)19 149 to 186. No room for primers.
PCR Comments :



Length of sequence : 191  Consensus sequence is :

..

GTTGGGGGGA GGGAGAGAAA GACGTATTGA GAGTGTGTGT GGTAGGCACA 
AAGGCACAAC TAGCGAGTGT GTGTGTGTGT GTTTGAAAGA GGAGTAGATT 
GACTATTATC AAGATCGATG TGCCACCCTA CTCCCTCAGT GCCGGGTAGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTTTGT G

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wmc(26c3) Plate reference : 26c3
WMC locus number : WMC Length of sequence : 137
Last updated : Ven 12 FŽv 1999 at 15:33
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 45 to 137 (complement) show homology to 1e7 bases 48 to 140 (93 in longest run, maxScore = 43). (GA)10 75 to 94. Duplicate, 1e7 has precedence.
PCR Comments :



Length of sequence : 137  Consensus sequence is :

..

CAAGTAAATA AAGGTGCATG TGTGTGTGAG ATGGAGTCTT AGTGTGTGTG 
TGGGGTGTGT GTGTGTTTGT GTGTGAGAGA GAGAGAGAGA GAGATGGAGT 
CTTAGTGTGT GTGTGGGGGG GGGCTGTGCG TGCGTGC

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wmc537(26d3) Plate reference : 26d3
WMC locus number : WMC537 Length of sequence : 228
Last updated : Ven 12 FŽv 1999 at 15:48
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 66 to 121 (complement) show homology to 3e3 bases 837 to 892 (56 in longest run, maxScore = 47). Primers have apparently no homology to 3e3. Bases 8 to 228 show homology to 26e3 bases 7 to 227 (221 in longest run, maxScore = 133). (CA)26 157 to 208, (CT)10 125 to 144.
PCR Comments : 51C anneal, polymorphic on acrylamide (98-106 bases and 176-184 bases).
Forward primer details :
TcTTcTgTAcATTgAAcAAcgA at 56 length 22 bases, Tm 58
Reverse primer details :
ATgcAgAAccgTgATAggAT at 208 length 20 bases, Tm 59
Expected size of amplified fragment (Chinese Spring=) 170 bases.



Length of sequence : 228  Consensus sequence is :

..

AATATTCATA TGGATGGAAC CTAGGATCGT GGTGTGATGC ATTCTACTTC 
TAGGATCTTC TGTACATTGA ACAACGATTT TTTCTAACAT TTCCCTTCAC 
AAGCTTGCTT CTTCTTCATT GCATCTCTCT CTCTCTCTCT CTCTCCCTCT 
CTCTCTCACA CACACACACA CACACACACA CACACACACA CACACACACA 
CACACACATC CTATCACGGT TCTGCATC

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wmc(26e3) Plate reference : 26e3
WMC locus number : WMC Length of sequence : 227
Last updated : Ven 12 FŽv 1999 at 16:04
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Bases 7 to 227 show homology to 26d3 bases 8 to 228 (221 in longest run, maxScore = 133). (CA)26 156 to 207, (CT)10 124 to 143. Duplicate, 26d3 has precedence.
PCR Comments :



Length of sequence : 227  Consensus sequence is :

..

AATATTATAT GGATGGAACC TAGGATCGTG GTGTGATGCA TTCTACTTCT 
AGGATCTTCT GTACATTGAA CAACGATTTT TTCTAACATT TCCCTTCACA 
AGCTTGCTTC TTCTTCATTG CATCTCTCTC TCTCTCTCTC TCTCCCTCTC 
TCTCTCACAC ACACACACAC ACACACACAC ACACACACAC ACACACACAC 
ACACACATCC TATCACGGTT CTGCATC

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wmc(26f3) Plate reference : 26f3
WMC locus number : WMC Length of sequence : 505
Last updated : Ven 12 FŽv 1999 at 16:06
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to Ssp1 at both ends of insert. Bases 419 to 446 show homology to 5h7 bases 229 to 256 (28 in longest run, maxScore = 137). (CA)4 8 to 15, (CA)21 18 to 59. No room for primers.
PCR Comments :



Length of sequence : 505  Consensus sequence is :

..

AATATTACAC ACACATACAC ACACACACAC ACACACACAC ACACACACAC 
ACACACACAC TGCACACACA CTCCCCCAAC CTCGGGCACA CAACTCATCC 
CCATCTGAGG TTATATGGCG ACTACTCTCA CTTGTGCTTC ATTGCACCTC 
AGCATGCACA CCACCTCAAT TTTCAAAGAG GGCATTGAGG AGATCAAAGA 
CGGTGACCAC ACCACACAAC ATGTCTCCAT ATTTCTCACA GACCTAACTT 
CTTCCTTTCT CTCTCTCACA TTAGGTTTGT CTCCTACTCT CTCGCTCACA 
TACGTACAAT CTTTCACTCC CTATCTGCTC CATCTTCGCA AGCTCTCTCT 
ACATGTTTCA TTCACACATT GGGATGTGTT CAAAATGTTG CTTTTATAAT 
AGATCATGTA GAGTTATGGT TGGTTTTGTT GCATCCTACA AAGTAAGAAC 
TATGAAATGC ATTTAGATTA TCATTTGACT GGGATTATGT GAGTTTGAGA 
ATATT

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wmc(26g3) Plate reference : 26g3
WMC locus number : WMC Length of sequence : 187
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 187  Consensus sequence is :

..

AATATTAGTG ACCCGTGCCG CCCCCGCCGC CGCCCCCACC GGCCGGCGGC 
CATCACGGCG CAGAACGCCC CGGAAGGAAG TGACTCAGCC CCTCCCCATG 
CTCACCGTGC AGTTTAAGGG ATGGTCATAT TTTGCGAAGG GTTTATGAAT 
TCAGGAGAAA ACACTCAATG AGCTGGATGG AACCTCA

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wmc(26h3) Plate reference : 26h3
WMC locus number : WMC Length of sequence : 157
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 66 to 82 (complement) show homology to 5b11 bases 327 to 343 (17 in longest run, maxScore = 20). No substantial microsatellite.
PCR Comments :



Length of sequence : 157  Consensus sequence is :

..

GTGGTGGCGG CCAAAACCCT AGCAGCGGCT ACCCTGGCGC GGGTAGTGGT 
GGCTACAACC ACGGCGGCGG CGGCTACAAC AATCAAGGCA ACAGCTGCGG 
TCAAGGCGGC AGCCACCATG GCGGCGGTGG TGGAAACTAC AACAACAACC 
ATGGCGG

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wmc(26a4) Plate reference : 26a4
WMC locus number : WMC Length of sequence : 357
Last updated : Ven 12 FŽv 1999 at 16:11
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 140 to 210 (complement) show homology to 22c5 bases 33 to 103 (71 in longest run, maxScore = 123). (CA)6 8 to 19. No room for primers.
PCR Comments :



Length of sequence : 357  Consensus sequence is :

..

GATTATACAC ACACACACAC GCATGCACAC GAACCCCATC CACGCATCAC 
TCACACAAAC ACATGCGCCC ACGCATGCAC GCAACACTCA CACGTACACA 
CACAGCCATG CACGCACACA ACACTCACAT GCACACACGC ACGCATGCAT 
GCACACACCA GTACACCACA CGACCAACAC ACACTCTCAC GCTCAAGTGC 
TCAACCTACA TGTGCATGTG CACACATCAG GCCCTGACAG ACACATACAC 
AACCAGGTGA TTCCCGCACA CGGGTTGGAG CCTTGTCGCG CTTGCGTAAA 
TTCGTGTTTA TCTTACCTTT TCCCTATGCG AACTGACAGG TGGGATCCAC 
AAGGAAC

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wmc(26b4) Plate reference : 26b4
WMC locus number : WMC Length of sequence : 300
Last updated : Ven 12 FŽv 1999 at 16:11
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)13 1 to 26. No room for primers.
PCR Comments :



Length of sequence : 300  Consensus sequence is :

..

CACACACACA CACACACACA CACACACTAT GATCAACAAC AGCAGAAAAA 
CAACAAGAAC AAAGCAAAAT CAAGAAAACA ACAGGCAGAC CATAAGCAAC 
TAGGCATAAG TTTAAAACAG CTGGGCATCC CATGCCCTGC AGGACCCCAG 
GGTGCTGCTC CCTGGCCAAA ATATGGACAT GTTCACAGCA AAAGAGACGA 
CCGGGCTTAG TTCAAACCTA CACTACTTCA GTTCAGACTA CGAGCATTAG 
AGATCAGACT TGAGAGACGA ACTAGAGGGC CTGAGTGATG CCGACGCCTG

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wmc(26c4) Plate reference : 26c4
WMC locus number : WMC Length of sequence : 260
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to Ssp1 at 5' end and to insert at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 260  Consensus sequence is :

..

AATATTCATG GATGGGTCCT TGGCCGAAAT AACAGAAAAA GAAAAGAACT 
AAATAACGAA CGTTCATTTA TAAGATCTAA ACAGTGGATG TTCGCTATTT 
AAATAAACCT AAAAAAACAG ATCTAGTCTA GGGTCTTAAA AAAACCGAAG 
TTAAAAGAAA AACCGGATCG GACCGGCGGT TTTATACCGG TTCGGGAGGT 
TTTGACGACG GTGGTGATTT CCGGCGAGAC AGGATGGCGG CGGCGGCGGC 
TCGGGTCCGG

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wmc(26d4) Plate reference : 26d4
WMC locus number : WMC Length of sequence : 286
Last updated : Ven 12 FŽv 1999 at 16:21
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 286 show homology to 26e4 bases 1 to 286 (286 in longest run, maxScore = 228). No substantial microsatellite. Duplicate with 26e4, 26d4 has priority.
PCR Comments :



Length of sequence : 286  Consensus sequence is :

..

TCCATGCGGT TGGCGTGCGG TGATGCCCCA GGAAGCGCTG GCGCCGGAGC 
AGTGGATGGT GGTGCGGTCA GATCCATTCG ATCTGGGCCT GTCGGGTTGG 
TTTTGCCGCG CGGCAGTGGA AAGACCACGG CTTTCTTCTC GCGGACGGCG 
CGGGGATTCA CGGTGTGGAT GGAGGCACAA CGGAGGTCAC GGCGAGGGCG 
TTCGCCGTAT CCTGGCCAGG TGCACCTCTG GTAGGTGGCG GCAGGTGGAG 
CGCCAGCAAC GGCGGTGGCG GCGGCGTTGG ATCTGC

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wmc(26e4) Plate reference : 26e4
WMC locus number : WMC Length of sequence : 286
Last updated : Ven 12 FŽv 1999 at 16:21
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 286 show homology to 26d4 bases 1 to 286 (286 in longest run, maxScore = 228). No substantial microsatellite. Duplicate, 26d4 has precedence.
PCR Comments :



Length of sequence : 286  Consensus sequence is :

..

TCCATGCGGT TGGCGTGCGG TGATGCCCCA GGAAGCGCTG GCGCCGGAGC 
AGTGGATGGT GGTGCGGTCA GATCCATTCG ATCTGGGCCT GTCGGGTTGG 
TTTTGCCGCG CGGCAGTGGA AAGACCACGG CTTTCTTCTC GCGGACGGCG 
CGGGGATTCA CGGTGTGGAT GGAGGCACAA CGGAGGTCAC GGCGAGGGCG 
TTCGCCGTAT CCTGGCCAGG TGCACCTCTG GTAGGTGGCG GCAGGTGGAG 
CGCCAGCAAC GGCGGTGGCG GCGGCGTTGG ATCTGC

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wmc(26f4) Plate reference : 26f4
WMC locus number : WMC Length of sequence : 330
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 330  Consensus sequence is :

..

CTCTGCAAAA TTCTCGCACA TGGCCGGTGA ATGTTTAGCG GTTTGGCGGC 
GGCGGTCTCC AGGGGAGGCG GCGGCGGCGA GAACGTGTTC CACGGGATGC 
TGACGGTGGT GAGCAACCCG AGGAAAGACG GCAGCCCTGC CGGAGTATGG 
ACATATTGTT TAGACGTGTC AGGTGTACAT ACATTCAAAC AGATTGTTCT 
GAACCCAATG ATCTTTCTGA ATGTTACAAA GTATTTACAT GTAGTTGTCA 
CTCCCTTCAG CTTCAGCTGA GGGTTGAGTT GTCTCAAACA AAAAAAAAAG 
ATGAGGGTTG AGCCCTATAT CACAAATATT

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wmc(26g4) Plate reference : 26g4
WMC locus number : WMC Length of sequence : 295
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 295  Consensus sequence is :

..

CGAACAGAGA GCGGGGACTC CTTGGAGTAG GCGATCCTCC TCTCCTCCAC 
CCTGCGTACG TTAACTAGCA AGACCGCGTC ACGAACCACG CATCAAAGAC 
GCGCGCACAT GCTGCTACTC TGCACACATC GGCGCGTACA GAACTGGGTA 
GTACAGTACC TGGGAGGAGT ATCGGGGTCA GGGGTGGAGC TCATCTCGAC 
GGCGGACGGC GGACGGCGGC GGCGGAAGGA GCGCAGCTTG TGTGAGCAAC 
ACTGCTAAGA TCCACAGCTT TATTACGGCG ATCCCGAACC GGCCT

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wmc538(26h4) Plate reference : 26h4
WMC locus number : WMC538 Length of sequence : 423
Last updated : Ven 12 FŽv 1999 at 16:33
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 323 to 374 (complement) show homology to 17f9 bases 50 to 101 (52 in longest run, maxScore = 79), primers have no apparent match to 17f9. (GT)17 15 to 48.
PCR Comments : 51C anneal, product (80 bases)
Forward primer details :
AgccATTgAAATTgTgT at 2 length 17 bases, Tm 51
Reverse primer details :
TgATcTATgcccTcTgA at 87 length 17 bases, Tm 52
Expected size of amplified fragment (Chinese Spring=) 102 bases.



Length of sequence : 423  Consensus sequence is :

..

GAGCCATTGA AATTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGC 
AAGTCAAAGA TATAGGGCGA CCGACCTACC GGAACATCAG AGGGCATAGA 
TCAAGTTTGT GTGTGGCAGG GAGACATGAC TAGAGCGCTC GATGTATTGG 
TGTGTATGTG TGGGAGGGGG GAGGGTAGGC AGAGGCCTAA CTACAGAGGT 
AGAACGACTC GTATATGTGT TGAGAAGGAG AGACGCAGCT ATGTCTTGAG 
GGAGATCGGT CGACATCCAT ACATAACTGA AGGACGGGAA ATAGGATGGG 
ACAGAGAGAG AGGATAGAGA GAGAGGGAGG GAGAGGGGTA GAGTGCTGGA 
TGGGTGATGG AGTTGCTTCT CGAATATGGT GGGAGAGGCC TACTAGACAA 
ACTGAGGGTG GAACTGCAAT ATT

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wmc(26a5) Plate reference : 26a5
WMC locus number : WMC Length of sequence : 195
Last updated : Ven 12 FŽv 1999 at 16:35
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 131 to 183 (complement) show homology to 2A1 bases 137 to 189 (53 in longest run, maxScore = 96). (CA)16 1 to 32. Near duplicate with 2a1, 2a1 has precedence.
PCR Comments :



Length of sequence : 195  Consensus sequence is :

..

CACACACACA CACACACACA CACACACACA CAAACGCGCG CGCGCCCGTA 
GCATAACACA CACACACACA CTCCCCCGAG CACACAATTC GTCCCCATCC 
AAGGTTATAC GGTGACCACT CTCGCTTGTT CTTCTTTGCA CCCCAACATG 
CACAACACCT CAATCTTCAA AGAGGGCATC GAGCAGATCG AAGAC

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wmc(26b5) Plate reference : 26b5
WMC locus number : WMC Length of sequence : 244
Last updated : Lun 8 FŽv 1999 at 11:03
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 164 to 220 show homology to 2f3 bases 210 to 266 (57 in longest run, maxScore = 31). (GA)8 107 to 122, (GA)6 173 to 184, (GT)8 218 to 233. No room for primers.
PCR Comments :



Length of sequence : 244  Consensus sequence is :

..

GAGCCCGGAC TCGGCATCAC CTCTGGGTGA TCAGCCCTGC TCCAGCTGAT 
AGGCTTCTCG GACCAGTGCA TGAACTCTAG CGTCTCTGAT GCTACTGCAT 
TCACGTGAGA GAGAGAGAGA GAGTGAGAGA GAGAGATGGA GTCTTAGTTT 
TTGTGTGTGT GTATGTGTGT GTGAGAGAGA GAGATGGAGT CTTAGTGTGT 
GTGTGGGGGG GGGGTGCGTG TGTGTGTGTG TGTTTGTGTG TGTG

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wmc(26c5) Plate reference : 26c5
WMC locus number : WMC Length of sequence : 6
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : No sequence 20.1.99
PCR Comments :
No sequence available for this clone
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wmc(26d5) Plate reference : 26d5
WMC locus number : WMC Length of sequence : 6
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : No sequence 20.1.99
PCR Comments :
No sequence available for this clone
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wmc(26e5) Plate reference : 26e5
WMC locus number : WMC Length of sequence : 211
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. No substantial microsatellite.
PCR Comments :



Length of sequence : 211  Consensus sequence is :

..

GGAGGTGGCG GCGGCGTTCT TCATGGACAG GAACTGCGCG TGTGCGGCGA 
GCGCGGGGCG CGGGGCGGCG AAGCGAGAGG CGAGGTTGGA TAGGTAGGAG 
GAGGCGACAA CGAGGGGGTG GGAGGGGCAG AACGTGCGGC GGTGGCGGCG 
ATCTGAATCG AGGGAGAGGA TGAGAGGGGA TCGATGGGAG TTAGGTTTTG 
GGGTTGTTGG G

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wmc(26f5) Plate reference : 26f5
WMC locus number : WMC Length of sequence : 53
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 5' linker corrupted). No substantial microsatellite.
PCR Comments :



Length of sequence : 53  Consensus sequence is :

..

CGGGCGGGAA GCGACGATGG GCGGCAGGGG CCGATGACGG CGACGAGACG 
GGC

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wmc(26g5) Plate reference : 26g5
WMC locus number : WMC Length of sequence : 90
Last updated : Ven 12 FŽv 1999 at 16:39
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted). Bases 1 to 50 (complement) show homology to 2d2 bases 40 to 89 (50 in longest run, maxScore = 42). No substantial microsatellite. Duplicate, 2d2 has precedence.
PCR Comments :



Length of sequence : 90  Consensus sequence is :

..

AGAGAGTGAG TGTGTGAGAT ACACATGCAG ACATGAGGAG AGATGAGGAT 
ATAGGTAAAT GGCTGTTTGT GCATGTTTGT GTGTATTTGT

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wmc(26h5) Plate reference : 26h5
WMC locus number : WMC Length of sequence : 391
Last updated : Ven 12 FŽv 1999 at 16:40
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)27 337 to 390. No room for primers.
PCR Comments :



Length of sequence : 391  Consensus sequence is :

..

CGCCGGTCAT CGATCGCATC GCCAACAACA CCTGCCATAG TCGCCGGCAC 
GATTGGCACA TCTCGGTTGG AGCTTCTACC TTTACCAGCT GGTAGATACT 
TCAAGATAAT TACATTTTTC TCAGAGGATT TGGCTTGATG GGTGGGTTAG 
AAATTGCCTG ATACGCCATA GTACATGCCA TTTTAGAAGC GCAAAACAAA 
TTATGGGTCG CCGATGTAAA CGGCAAATTT AAAGTTTGTA TCGCTACATT 
GTATGGCATG TTTAGACATG ACAAAGCCAT GGATGGTGAC GCTATAGAAT 
AACACGTTTT ACCTAGGACA TTGGCGCGCG CGCGCCCACA CACACACACA 
CACACACACA CACACACACA CACACACACA CACACACACA C

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wmc539(26a6) Plate reference : 26a6
WMC locus number : WMC539 Length of sequence : 225
Last updated : Ven 12 FŽv 1999 at 16:45
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)64 75 to 202.
PCR Comments : 51C anneal, product (200 bases).
Forward primer details :
gcAAgTAggAccTTAcAgTTcT at 27 length 22 bases, Tm 56
Reverse primer details :
gTTATAAccTTTgTcccTTcAc at 204 length 22 bases, Tm 56
Expected size of amplified fragment (Chinese Spring=) 199 bases.



Length of sequence : 225  Consensus sequence is :

..

CCTGTGGTAT TTTTAGCAAA GAAAATGCAA GTAGGACCTT ACAGTTCTTT 
TTCTTTCTTC TTCATCATTT ACAAGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTTGTGAAGG GACAAAGGTT ATAAC

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wmc(26b6) Plate reference : 26b6
WMC locus number : WMC Length of sequence : 79
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at 5' end, 3' end not located. Bases 31 to 79 show homology to 4h4 bases 174 to 222 (49 in longest run, maxScore = 42). No substantial microsatellite.
PCR Comments :



Length of sequence : 79  Consensus sequence is :

..

TAAAAATGAC TAGAGAGGCC CATATAAGAG CACGCGCCTC GAGGGATCCT 
CTAGAGTCGA CCTGCAGGCA TGCAAGCTT

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wmc(26c6) Plate reference : 26c6
WMC locus number : WMC Length of sequence : 221
Last updated : Ven 12 FŽv 1999 at 16:45
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)24 9 to 56. No room for primers.
PCR Comments :



Length of sequence : 221  Consensus sequence is :

..

CGAAGTTACA CACACACACA CACACACACA CACACACACA CACACACACA 
CACACACTGA AGATTAATTT GCAACATGCA AATTTGGTAA GGTCCACCTA 
GACAAATTAA TGCAAGACAA GAGACGGGCC TGCGGCAACC ATAGCCTGTT 
GTAGCGACCT TCCCCCTCAC CTGCCACTCT CCCTCGTTAT TGCCGATGGT 
GGCCTCTTGG CAATGCATGA C

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wmc(26d6) Plate reference : 26d6
WMC locus number : WMC Length of sequence : 211
Last updated : Lun 8 FŽv 1999 at 10:07
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 83 to 121 show homology to 10a4 bases 308 to 346 (39 in longest run, maxScore = 95). No substantial microsatellite.
PCR Comments :



Length of sequence : 211  Consensus sequence is :

..

GCTGAGTTGG CGATGATGGT GTGCCTGCCA TCTTGTAATA TAGACCGTCT 
GATCTATATC TGACAGATAG AAAGCAAACT ATGATAATTT TACAAAAGAT 
ACCCGCACCT CTCTCCACAT CCTTATCTCC CACAACCTCA TTGCTGAGCA 
ATTAAAAAAG AAAGTCTCTG GAACAATCTA GCATGGTGGC CGCCGCCGCC 
TGCCGACGCC G

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wmc(26e6) Plate reference : 26e6
WMC locus number : WMC Length of sequence : 6
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : No sequence 20.1.99.
PCR Comments :
No sequence available for this clone
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wmc(26f6.1) Plate reference : 26f6.1
WMC locus number : WMC Length of sequence : 250
Last updated : Mer 3 FŽv 1999 at 14:59
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. No substantial microsatellite.
PCR Comments :



Length of sequence : 250  Consensus sequence is :

..

CGGCTGCCAG ATCCAACCGC TCCACCGCCT CTGCCATCGG TTTCGCCGCT 
CTCTGCCGTC TCCCGCCTTC AAATTGGCCG CTTCTTCCCT GCCGAATCGC 
CGCTGTGCCG CCGCATGATT TGAGCCCCCC CACCACACCG CCTTGCTGCC 
AGATCCAACC GCTCCACCGC CTCTGCCACC AGCACCGCCG CTCTCCGCCG 
CTGCCCTCCT TCAAATTGGC CGAATTGGCC GCTTCCCCCT ACCGAATATT

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wmc540(26f6.2) Plate reference : 26f6.2
WMC locus number : WMC540 Length of sequence : 339
Last updated : Ven 12 FŽv 1999 at 16:57
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 186 to 204 show homology to 8f3 bases 123 to 141 (19 in longest run, maxScore = 43). Reverse primer has 6 mismatches to 8f3, forward primer no apparent homology. (GT)9 186 to 203.
PCR Comments :
Forward primer details :
cggggTccTAAcTAcggTgA at 75 length 20 bases, Tm 64
Reverse primer details :
ccTgTAATggAggAcggcTg at 277 length 20 bases, Tm 65
Expected size of amplified fragment (Chinese Spring=) 222 bases.



Length of sequence : 339  Consensus sequence is :

..

AATATTGAGA GGACGGGTCC CACCTGTCGT GGTACTAAGT CTGGCAGTAA 
GAATAGGGGG TACGTATGAG GAGGCGGGGT CCTAACTACG GTGAGGTTGT 
ACACACGAGT TTACGAGTTC AGGCCCTTCT CGCAGGAGGT AAAAGCCCTA 
TGTCTCGGTG CCAGAGGCGG TCGACTGGAA TATGGGTGTG TGTGTGTGTG 
TGTGGGTGTG TGTTACAAGG GGTGCGAACC CTTGTCCCAG AGGAGGGGGT 
AGCTTATATA GGGTGCGCCA GGACCCCAGC CGTCCTCCAT TACAGGGATT 
CATTGTACAT TAAGATAGGG CGTTACTGGT AACGCCAGC

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wmc(26g6) Plate reference : 26g6
WMC locus number : WMC Length of sequence : 351
Last updated : Ven 12 FŽv 1999 at 17:08
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 127 to 146 show homology to 11f12 bases 412 to 431 (20 in longest run, maxScore = 44). (GT)12 255 to 278, (GT)19 281 to 318. Too difficult to make good primers, minisatellite like repeats near this sequence.
PCR Comments :



Length of sequence : 351  Consensus sequence is :

..

CAAGTGTACC GTCGACTCGC AACTAGGTGG GGAGTGTGTT TGTAGGGGGC 
GGTTGCAGGT CAACTATCGA CTCGCAACTG GAGGGGGGCG GTATATGTCT 
TTTGTAGGGG GGCAGTTGCA TGTCAACCAT CGACCCGCAA CTAGGGGGTT 
GTGTGTTATT GTAGGGGCCT TAGTTGCAAG TCTACCATTG ACTCGCAACT 
AGGGGGGAGT GTGTTTGTCG GGGCTCCCGC TATCAACTCG CAAACTAGAG 
AGATGTGTGT GTGTGTGTGT GTGTGTGTAT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTTT GTATGGGCCC AGTTACAAGT CAACCATCAA 
C

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wmc(26h6) Plate reference : 26h6
WMC locus number : WMC Length of sequence : 234
Last updated : Ven 12 FŽv 1999 at 17:10
Owner : Madre, Secobra Date at Agrogene : 20.1.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 65 to 113 show homology to 6f9 bases 184 to 232 (49 in longest run, maxScore = 41).(GT)7 7 to 20, (GA)8 23 to 38, (GA)8 137 to 152, (GT)9 163 to 180, (GT)9 195 to 212. No room for primers.
PCR Comments :



Length of sequence : 234  Consensus sequence is :

..

GTCTTAGTGT GTGTGTGTGT CTGAGAGAGA GAGAGAGAAA GAGAGATGGA 
GTCTTAGTTT GTGGGTGTGT GAGAGAGAGG GAGAGAGATG GAGTCTTAGT 
GTGTGTGGGG GGGCGTGTGT GCTTGCGTGT GTGTGTGAGA GAGAGAGAGA 
GATGGAGTCT TAGTGTGTGT GTGTGTGTGT TGGGGGGTGC GTGCGTGTGT 
GTGTGTGTGT GTCGCGGGGT CCTCTATGGC GGAT

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wmc(10b4) Plate reference : 10b4
WMC locus number : WMC Length of sequence : 495
Last updated : Lun 5 juil 1999 at 17:22
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 476 to 495 (complement) show homology to 2e8 bases 37 to 56 (20 in longest run, maxScore = 27). No substantial microsatellite.
PCR Comments :



Length of sequence : 495  Consensus sequence is :

..

GnGCnGACCn TTnCGGATnG ATCTAGnnCT CTCCCTCCAA CTACGACACA 
AATCAATCAT CCAATCTCCT CTCGTTAGGC TTGAAGGCGG ATGGAAAGGG 
AGCGGcGGAA AAGGCGGCGA CGGACTGGCG AGTGGCGGGA AGGCGGCGAC 
GGACAAGGGA GCGGCGGCTA TGCGGACAGC ACAAAAAGGA GAAGAAGTAA 
GGCAGCGGTA GGAGGGCTGG AAGTCATCTG CTAGCTTCCA CTGCTCCTCG 
GGCCGCCACT CCGACCTGCC CGCCGTCGGT GCGCTGCTCC TCCGGCCGGC 
CATCTTCTCC CTCGCCGTGC AGGACAGCAA GACTGACCAC CGAGGTAAAC 
CTCCTTCCTT CCCTTCTCCT CTCATCTAGT CAACTAATTT GTGGGCACCT 
GGAGACATGG CCGTGGGCTG TAGGGGGATT TGGTAGATGA CGAAGGGGAT 
GGATTTCAGA GCTgttagtc cacgggcctc gagggatcct ctaga

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wmc(10c2) Plate reference : 10c2
WMC locus number : WMC Length of sequence : 185
Last updated : Lun 5 juil 1999 at 17:22
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to insert at 3' end, 5' end not located. Bases 56 to 95 show homology to 10b12 bases 82 to 121 (40 in longest run, maxScore = 44). No substantial microsatellite.
PCR Comments :



Length of sequence : 185  Consensus sequence is :

..

GAnAAnAAGC TGTCnACCAA ATCTTTGnnn TGnTAGGAGA AGCnCTCGGT 
CGAnACACGA TCCGGGGCCT tnaGGGAAGG CATGAAnGAn AnCCCnCnGn 
CTACTnGnAA GAAAnCGnCG ACGGnnAnGG TACCGGCGGC TATGCGGCAG 
nACATnAAGn AGAAGCCTGG ATCGGAGACg AGGnC

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wmc541(10c4) Plate reference : 10c4
WMC locus number : WMC541 Length of sequence : 475
Last updated : Lun 20 sep 1999 at 17:25
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Bases 100 to 140 (complement) show homology to 22c5 bases 356 to 396 (41 in longest run, maxScore = 87), classified as duplicate of 22c5, 2c5 has priority . (AC)7 264 to 277.
PCR Comments : 61C anneal, product.
Forward primer details :
TTccTcgTTcATccTTTTcTcT at 37 length 22 bases, Tm 61
Reverse primer details :
gTgTTTATgTgAgTgTTgcgTg at 298 length 22 bases, Tm 61
Expected size of amplified fragment (Chinese Spring=) 283 bases.



Length of sequence : 475  Consensus sequence is :

..

TTGGAACACA CCTCTCCGCA TTTGTAAATA AGGCCTTTCC TCGTTCATCC 
TTTTCTCTCA CAAGATAAAC CAGTCATACA AATGCATCTT CATGTTACGG 
TGCAACGCAC GGGCATCTTG CTAGTAAGAA TGAAAACAAA ACGACAAAAA 
ATTATTGGAC GGnGGTTCAA AGTCATGACC GCGGGCCCAA TAAACAAGGn 
GGCCTTGTAT nAnTATGCTG AGCCGTCTGT TTTAACACAC ATGAGCTGGT 
GTGGTGATTA TATACACACA CACACACGCn TGCnCATGAA CTGCATGCAC 
GCAACACTCA CATAAACACA TGCAGCCATT GCACACATTG CAACAnTCAC 
ACATACACAC GCAGCCACAC ACGCACCGTT ACACACACAT GCACACATAC 
ACACACGCAT GCATGGGACA CACCCACCAC GACCAACACA CACTCTTACA 
CTCAAnGAGC TAAACCTACA CGTAC

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wmc542(10c5) Plate reference : 10c5
WMC locus number : WMC542 Length of sequence : 301
Last updated : Mar 20 Juil 1999 at 11:40
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 157 to 179 show homology to 6b1 bases 191 to 213 (23 in longest run, maxScore = 98). Forward primer has 3 mismatches to 6b1, no obvious match for reverse primer. (CA)8 197 to 212, (CA)10 264 to 283, (CT)9 246 to 263.
PCR Comments : 51C anneal, polymorphic on acrylamide (very complex) .
Forward primer details :
cAATAAAATTAcTcTcTccc at 77 length 20 bases, Tm 50
Reverse primer details :
TgAAAgcTAgAgTTTggT at 283 length 18 bases, Tm 50
Expected size of amplified fragment (Chinese Spring=) 224 bases.



Length of sequence : 301  Consensus sequence is :

..

CACTTGTCTT TCACTCTGTC TTTCTCTCTA TAGGGTTCTC TCACACACCC 
TATATCTCTC TAGATATGAC TCATACCAAT AAAATTACTC TCTCCCGCAG 
ACACAACACT TGTTGCGGTC TCTTTTCCGT CTCCATCACA GTTATAAAAC 
TGACATTATT CATTTGTTTA CCGATCAGAT AAACTCCCCC CTCTCTCACA 
CACACACACA CAACTCCTGA TACCACTCCC CTACTCGACT ACCATCTCTC 
TCTCTCTCTC TCTCACACAC ACACACACAC ACACCAAACT CTAGCTTTCA 
C

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wmc(10c6) Plate reference : 10c6
WMC locus number : WMC Length of sequence : 261
Last updated : Mar 20 Juil 1999 at 13:37
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end 5' ernd not located. Bases 143 to 161 show homology to 3b11 bases 111 to 129 (19 in longest run, maxScore = 56). (GT)10 201 to 220. Near duplicate with 3b11, 3b11 has precedence.
PCR Comments :



Length of sequence : 261  Consensus sequence is :

..

actaAAnnnG GnCTTTGGCA GGCGTTATCT AAGAAACTCA AGGCGGCGTG 
GGTATGGAnA TCGGTGGCTA ATCTAGACAA ACTAACCGGC ATATGTTGAC 
nATGGATGCC TTGCGGCCCC GGCGGCGGCG GCGGCGGGGA CCTTGCTAGG 
GTTTTGAGCG AGGGAGGGGG TGTGTGTGCG CGCGCATGCC CGAGGTTTTG 
GTGTGTGTGT GTGTGTGTGT GGAGAGGGGG CGCGGGGTGT TTGAGGAAAT 
GACACGGGTA C

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wmc(10d2.1) Plate reference : 10d2.1
WMC locus number : WMC Length of sequence : 370
Last updated : Mar 20 Juil 1999 at 13:59
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at 3' end, 5' end not located. Bases 251 to 302 (complement) show homology to 5b8 bases 89 to 140 (52 in longest run, maxScore = 82). (CA)6 88 to 99, (CA)7 169 to 182, (CA)6 195 to 206. Near duplicate with 5b8, 5b8 has precedence.
PCR Comments :



Length of sequence : 370  Consensus sequence is :

..

TGGAACACAC TCACACTTTC CAACACTGAA GGAGTAGGGT GACACCTCGA 
TCTTGATACT AATCTATCGA CTAGCTTCTC TCTCAAACAC ACACACaCAC 
TACCCCGGCA CTGAAGGAGT AGGGTGGCAC ATCGATCTTG ATAATAATCT 
ATCTACTCCT ATTTCAAACA CACACACACA CACGCACACA CACACACTTG 
CTAGTTGTGC CTCTGTGCCT ACCGCGCACA CTCTTGATGC GTCTTTCTCT 
TCCCCCCCAA CAATGACAGC AGAAGGGTGA CAACTCGATC TGATCATAAT 
CTATAAATTG GTTTCTCTCT CAAATAGTGT GTCTCGGTGC CTCGCTCGGT 
AGCCCCCTCG ATATGTAGAC

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wmc543(10d2.2) Plate reference : 10d2.2
WMC locus number : WMC543 Length of sequence : 244
Last updated : Mar 20 Juil 1999 at 14:08
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to Rsa1 at 5' end 3' end not located. (GA)9 91 to 108.
PCR Comments : 51C anneal, complex product.
Forward primer details :
AccAcAAATcATATAATcgcAc at 32 length 22 bases, Tm 58
Reverse primer details :
TAccTTTcccTcTTATgTccTT at 223 length 22 bases, Tm 58
Expected size of amplified fragment (Chinese Spring=) 213 bases.



Length of sequence : 244  Consensus sequence is :

..

GTACAAACCT TACAGGTTCA CTCAAGCAAA TACCACAAAT CATATAATCG 
CACAACAATG GTTTGTGGTT CACAGAATTA CAGAAAAATT GAGAGAGAGA 
GAGAGAGAAC AGCAACAGGT TGGAGTTCAG AGAAGAACTG CATCAACGGG 
TTTGAGAAgA GCTTCAGAAG AAGTGCAGCA GCAGCAGCGC CCAGGAGAGA 
TGGGGACAAG CCACAGGGCA GCAAGGACAT AAGAGGGAAA GGTA

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wmc(10d3) Plate reference : 10d3
WMC locus number : WMC Length of sequence : 460
Last updated : Mar 20 Juil 1999 at 14:12
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to Rsa at 3' end, 5' end not located. Bases 256 to 280 (complement) show homology to 5e6 bases 183 to 207 (25 in longest run, maxScore = 179). No substantial microsatellite. Near duplicate with 5e6, 5e6 has precedence.
PCR Comments :



Length of sequence : 460  Consensus sequence is :

..

GGACTCACGA CCGCTGGCAG GTCCGCTACC ACGACGCAGC GGCTAGGCTC 
AATTTCCCCT TCAGCACACG TnCGGTCCAC CTCGTTCCAC CGGAGCCAGG 
GGTGGAGAGC TCGGCTATGG CATGGGAGGA CCGCGAGGCG TGGGAGCATC 
TCAAGGCCGA GGCCGCCGAT GAGGCCTACA TGCAAGAGCT CCGCCGGCAG 
CACCCGGAGC TCGTGGAGGC GGTGCGGGCG ATCTTTGTCG GCGCGAAGGG 
CGGTGAGGTT ATCGCGCTCT CCTCCGATGA CGAGATCGAA GGCGGCGAGG 
ACGGCGGGGC GGAGGGCGGC GAGGTTATCA TGCTCTCCTC TGATGGCAAG 
GTCGAAGGCG GCGACGGCGG CGCAGAGGGC ATCGAGGTGG GCCCTTCGGA 
CGAGGAGATC GATGTCGACG AGTGGAgGAG TGACTTCCCA ACGACCCCGA 
CGACCGGTAC

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wmc(10d4) Plate reference : 10d4
WMC locus number : WMC Length of sequence : 331
Last updated : Lun 5 juil 1999 at 15:54
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. No substantial microsatellite.
PCR Comments :



Length of sequence : 331  Consensus sequence is :

..

CnCCGnCnGC TTTGGTCAGT GGTCCnCTCC GTGTTGTGGC TATCTCCGGC 
GATGGGCGAG TGGGTGgggC GGCTAAGCGG GACGAGGGtt ATTCGnnnGA 
CnTnGCGGGA GGAGCATAGG ACGCTCAGGT TGGTCGGAAC CAAGCCGAAG 
CCgAgGCAgC CATGGAGGAT GACAGCGAAC AACAGCGAGA TGAGGCGACT 
CCAGAgGGCA AATGAnGCGG nGGAGGGATG AATGAGATnC TAGGGGTTCG 
GGGTCCTTAT ATAnGCGCnn ATGTGGGCTC TAGAGCATTG CTnGCGAGCT 
nnAnCATnGC TCTAATGGTG GACATTGGTA C

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wmc544(10d5) Plate reference : 10d5
WMC locus number : WMC544 Length of sequence : 492
Last updated : Mar 20 Juil 1999 at 14:22
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 143 to 163 (complement) show homology to 17e12 bases 355 to 375 (21 in longest run, maxScore = 55). Forward and reverse primers no obvious match to 17e12. (CA)14 104 to 131, (CA)10 238 to 257.
PCR Comments : 61C anneal, polymorphic on acrylamide.
Forward primer details :
ccATTTgAggTTTggTcgcTAc at 65 length 22 bases, Tm 64
Reverse primer details :
TATATgTgATTTgTcgTgcccc at 425 length 22 bases, Tm 63
Expected size of amplified fragment (Chinese Spring=) 382 bases.



Length of sequence : 492  Consensus sequence is :

..

gtggactcaC TGTGGGTGTC AGCATTACTG ATCCAGGGAT GACACTGGTG 
CACAGCAGCA CAGACCATTT GAGGTTTGGT CGCTACAGGG GACCCTnnAC 
AAACACACAC ACACACACAC ACACACACAC ACTTGTAGTT GTGAGTTGAT 
GGTTGACATG CAACTGGGGA CCCTCGACAA ACACGCACAC CCCTAGTTTC 
AAGTCGATGG TTGACAAGCA ATTGGGAACC CTCCAAACAC ACACACACAC 
ACACACACTC ACACTCACAC TCACACATAG TTGTGAGTCG ATGGnTGACA 
TGCAATGGGG ACCCTTGACA ACACACACAC ATCCCTAGTT GCGAGTCGAT 
GGTCGGGTTG CAATTGGGGA CCCTTGACAA ACACACACAC CCTAATTGTG 
AGTCGATGAT CGATATGCAA CTGGGGGGCA CGACAAATCA CATATACTCG 
TAGTTGCGAG TnGATGGTCG ACATGnAACC GGGGACCCnT CT

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wmc(10e3) Plate reference : 10e3
WMC locus number : WMC Length of sequence : 279
Last updated : Mar 20 Juil 1999 at 14:25
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end; 5' end not located. Bases 81 to 164 (complement) show homology to 7e4 bases 114 to 197 (84 in longest run, maxScore = 119). (CA)12 142 to 165. Duplicate with 7e4, 7e4 has precedence.
PCR Comments :



Length of sequence : 279  Consensus sequence is :

..

ggactaCATG GCATTGATGC AAAATGCGTT CAAACATCAA GCACACACCT 
CAAAACAAGG ATGCAACATG TCATGATGAG ACTACATGCA AAACCAAGCA 
AGTTTTAGAT AGGACATGAT CAAAACGGAG CAACGGTTCA ACACACACAC 
ACACACACAC ACACACGAAG CAAGTTTGAA TAACAATCTG CCCAAAACAA 
CAAGTAGGCA TATTGCAAGC ATCATAACAA CATGCTACAA GAACAAGATT 
CACACAAACA TGACATGCAC TTCAAgtAC

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wmc(10f3) Plate reference : 10f3
WMC locus number : WMC Length of sequence : 382
Last updated : Mar 20 Juil 1999 at 14:52
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to Rsa1 at 3' end, 5' end not located. Bases 115 to 133 show homology to 10g3 bases 269 to 287 (19 in longest run, maxScore = 65). (CA)27 238 to 291. Too difficult to make primers.
PCR Comments :



Length of sequence : 382  Consensus sequence is :

..

tggactCACA CACATTCCTA GTTGCGAATT AATGGTCGGA ATGCAACTGG 
GGGCCTCGAC AAACACACAC ACACCCTAGT TGCGAGTTGA TGGTCAGCAT 
GtAACTGGGG ACCCTCGACA AACACATACA CCCCTAGTTG CGAGTATTGG 
TCGGCATGTA ACTGCGGACC TTCGACAAAC ACATACACCC TTAGTTGCGA 
GTCGATGGTC GGCATGCAAC TGGGGACCCT CGACAAACAC ACACACACAC 
ACACACACAC ACACACACAC ACACACACAC ACACACACAC ACTTAGTTGC 
GAGTCGATGA TCGGCATGCA ACTGAGGACC CTTAACAAAC ACACACACCC 
CTAGTTGCGT GTCAAAGACT TGCAACTAgt AC

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wmc(10h1) Plate reference : 10h1
WMC locus number : WMC Length of sequence : 433
Last updated : Mar 20 Juil 1999 at 14:55
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 266 to 335 (complement) show homology to 1c11 bases 71 to 140 (70 in longest run, maxScore = 71). No substantial microsatellite. Duplicate with 1c11, 1c11 has precedence.
PCR Comments :



Length of sequence : 433  Consensus sequence is :

..

gactaactGT GCTGATGTGC ATGACAGGGC ATCGGCATGG CACACGGTTA 
GTGTGAGTGA ACCGTGTCTA TTGCGTCAAC CGACTTGTCT AATGTTCATA 
AATTTGGCGA AAATGACGCG GGAAAGGGTC AGAACACGAA CATACTTGTA 
TGTGGACCAA ATATATGTAT GAAGAGGTGG TTTGATGTTC AAATACCAAA 
TGCAACTTCG ATGTGGCACC TATCTCGCAA AGCGCACGGT ATTGCTTGCC 
CCCCTCATGT CGGCAGGAGG GGAATCCTAA GCTATGAACG CAAGCCCCCC 
CCCCACGGAG GGCCACCAAG CAAAGTGCGA AGTAGCAGTC CCCCCATCAC 
ACACCACACA CACACACACG CACGCGCAGA CACATACACA CACTAGTCCA 
CGTAATCCAC AGCGCCTCGA GGGATCCTCT AGA

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wmc(10h4) Plate reference : 10h4
WMC locus number : WMC Length of sequence : 681
Last updated : Mar 20 Juil 1999 at 14:57
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : No trimming done to sequence at Agrogene, ends not located. Bases 1 to 342 show homology to 10g3 bases 4 to 345 (342 in longest run, maxScore = 238). (CA)10 70 to 89, (CA)13 134 to 159, (CA)12 503 to 526. Duplicate with 10g3, 10g3 has precedence.
PCR Comments :



Length of sequence : 681  Consensus sequence is :

..

ggactaCAAA CACACACATC CCTAATTGCG AGTCGATGGT CGGGTTGCAA 
CTGGGGCCAT GACAAAAGAC ACACACACAC ACACACACAC CCTTAGTTGT 
GAGtCGATGA TCAGCATGCA ACTAGAGACC CTGCACACAC ACACACACAC 
ACACACACAG ACACACACAC ACACCTAGTT GTGACTCGAT GGTCGGCATG 
CAACTGGGGA ACCTCAACAA ACATCCCTAG TTGCGAGTAG ATGGTCGGCA 
TGCAACTGGG GGCCTTCGAC AAACACATAC ACCCTTAGTT GCATGTCGAT 
GCCCGGCATG CAACTGGGGG CTTCGATAAA CACACACACA TCCTAGTTGC 
AAGTCGATGG TTGGCATGCA ACTTGGGACC CTCGAGAAAC ACACACTCCC 
CTAGTTTCGA GTCGATGGTT GACATGCAAC CGGAGACTCT CGGAGAACAC 
ACATACACCC CTAGTTGTGA GTCAATGGTC GACATACAAC TAGGGGCCTC 
AACACACACA CACACACACA CACACACCTA GTTGTGAGTC GATGGGCTAG 
TTGCAACTAG GTGTCCTCAA CAAAACACAC ACCCCCTAnT TGCGAGTCnG 
ATGCTTGACA TGCCAACTGG GGACCCCTTG ACnAAAAAGA CCACACCACT 
nntccacgcg cctttgaggg atccctctag a

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wmc(10d1) Plate reference : 10d1
WMC locus number : WMC Length of sequence : 285
Last updated : Mar 20 Juil 1999 at 15:17
Owner : Gale, John Innes Date at Agrogene : 28.4.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. Bases 1 to 46 (complement) show homology to 11d4 bases 78 to 123 (46 in longest run, maxScore = 41). (GT)6 57 to 68, (GA)6 69 to 80, (GA)8 109 to 124, (GT)7 164 to 177, (GT)6 180 to 191, (GA)6 192 to 203. Too difficult to make primers.
PCR Comments :



Length of sequence : 285  Consensus sequence is :

..

CTCCGCCTAG AGCATGTGTG TGTGAGATGG AGTCTTAGTG TGTGTGGGGT 
GTGTTTGTGT GTGTGTGTGA GAGAGAGAGA TGGAGTCTTA GTTTGtGTGT 
GTATGAGTGA GAGAGAGAGA GAGATGGAGT CTTAGTGTGC GTGGGGGGTG 
TGCGTTCGTG CATGTGTGTG TGTGTGTTTG TGTGTGTGTG TGAGAGAGAG 
AGAGGTAAAG AGAGAGGGAG AGAGATGGAG TCTTAGTGTG TATGTGGGGG 
GGTGCATGCG TGTGTGTGTT TGTCGCGGGG TCCTC

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wmc545(26a7) Plate reference : 26a7
WMC locus number : WMC545 Length of sequence : 292
Last updated : Mar 20 Juil 1999 at 16:53
Owner : Momont Date at Agrogene : 18.5.99 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)11 235 to 256.
PCR Comments :
Forward primer details :
AAATgTTcAgAATTATTcATAgAc at 117 length 24 bases, Tm 53
Reverse primer details :
ATgTATTATgAATgAgcAcTTT at 270 length 22 bases, Tm 53
Expected size of amplified fragment (Chinese Spring=) 175 bases.



Length of sequence : 292  Consensus sequence is :

..

GGCACAAGCT GAAGAAGCGC CAAAAGATTT TCAGGAGCTG TCCAACAAAG 
TAGTCAGAGG TGAGAGGTGA GTCTTGCAGG TTCCCAGATG CAGTCATCCA 
TCCCTTTCTC ACACAAAAAT GTTCAGAATT ATTCATAGAC ATTTCTTAAA 
AAGGGAAACC AGCGAGATAA CAGTTATCTG CTAGAGTGCA CGCATAAAAG 
GTGCCATCTG TATTAAATGC AACATCAAAT TGTACACACA CACACACACA 
CACACATACA CACACAATTA AAGTGCTCAT TCATAATACA TG

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